CN104497131A - Method for preparing tannalbin by using egg albumin - Google Patents
Method for preparing tannalbin by using egg albumin Download PDFInfo
- Publication number
- CN104497131A CN104497131A CN201410807822.0A CN201410807822A CN104497131A CN 104497131 A CN104497131 A CN 104497131A CN 201410807822 A CN201410807822 A CN 201410807822A CN 104497131 A CN104497131 A CN 104497131A
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- ovalbumin
- water
- liquid
- albuminate
- tannin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/77—Ovalbumin
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for preparing tannalbin by using egg albumin, and the method is environment-friendly, simple to operate and low in cost. The technical method disclosed by the invention refers to react egg albumin with tannic acid to prepare a biochemical product. The technology has the advantages that on the basis of guaranteeing the product quality, no chemical reagent is added in the entire production process, the operation is simple, the cost is low, large-scale production is suitable and no environmental pollution is generated.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of method that ovalbumin prepares tannin albuminate.
Background technology
Tannin albuminate is astringing to arrest diarrhea medicine, does not decompose after oral in stomach, meets alkaline intestinal juice and then decomposites tannic acid; make intestinal mucosa vasoconstriction, inflammatory exudate reduces, and makes the protein coagulating on inflammation surface; form protective membrane, alleviate the hormesis of intestinal contents and reduce wriggling.Be used for the treatment of acute enteritis, Non-bacterial diarrhea, infantile dyspepsia.
Infantile diarrhea increases with times of defecation the UDC changing into feature with proterties, for world's public health problem, it is infant's common disease in period, the feature of tannin albuminate absorbs slowly exactly, nonirritant, without side effects such as gastrointestinal disorders, and the present invention in process of production, do not add any chemical reagent, be particularly suitable for children and use.
Summary of the invention
The object of this invention is to provide one ovalbumin and prepare tannin albuminate method.
Technical scheme of the present invention is: stirred through centrifugal, adding distil water by the fresh albumen that eggshell adheres to, add tannic acid liquid and staticly settle, drying precipitate pulverizes and sieves and obtains tannin albuminate finished product, comprises the steps:
(1) preparation of protein liquid: the fresh albumen that eggshell is adhered to, throw away ovalbumin by centrifugal, get ovalbumin gradation adding distil water, the ratio of ovalbumin and water is: 1Kg:1.5 ~ 2.5L, and at room temperature constantly namely stirring obtains uniform protein liquid;
(2) preparation of tannic acid liquid: get Turkey-galls fragment, adds the distilled water of 8 ~ 12 times amount, stirs, 50 ~ 60 DEG C of logical steam heating, soaks 6 ~ 8 hours, collects immersion liquid.Turkey-galls slag soaks 1 time again with method, filters, and twice immersion liquid merges, and leaves standstill, and the stillness of night is for synthesis;
(3) synthesize: tannic acid liquid is slowly added (by ovalbumin: Turkey-galls=1:1.5 ~ 3.0) in protein liquid, 55 ~ 65 DEG C of standing heating 50 ~ 80 minutes, make it combine completely, precipitation, abandoning supernatant, precipitates 1 time with equivalent distilled water wash, then moves into drying in hydro-extracting cage;
(4) dry: to get the block after drying; particle is made by 12 mesh sieves with granulator; be laid on drying tray; thickness is no more than 3cm; be placed in 75 ~ 85 DEG C of air dry oven dryings 6 ~ 10 hours; move into 125 ~ 135 DEG C of electric oven dryings 3 ~ 4 hours again, with high speed disintegrator, after crossing 60 mesh sieves, namely obtain tannin albuminate finished product.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment
The fresh albumen that eggshell adheres to by described utilization stirs through centrifugal, adding distil water, adds tannic acid liquid and staticly settles, and drying precipitate pulverizes and sieves and obtains tannin albuminate finished product, comprises the steps:
1. the preparation of protein liquid: the fresh albumen adhered to by eggshell, throws away ovalbumin by centrifugal, get 30Kg ovalbumin gradation adding distil water 60L, add for about 10 minutes, at room temperature constantly namely stirring obtains uniform protein liquid;
2. the preparation of tannic acid liquid: get Turkey-galls fragment 75Kg, add the distilled water of 10 times amount, stirs, 55 DEG C of logical steam heating, soaks 8 hours, collects immersion liquid.Turkey-galls slag soaks 1 time again with method, filters, and twice immersion liquid merges, and leaves standstill, and obtains the stillness of night for synthesis;
3. synthesis: tannic acid liquid is slowly added in protein liquid, 60 DEG C of standing heating 60 minutes, makes it combine completely, precipitation, abandoning supernatant, precipitates 1 time with equivalent distilled water wash, then moves into drying in hydro-extracting cage;
4. dry: to get the block after drying, particle is made by 12 mesh sieves with granulator, be laid on drying tray, thickness is no more than 3cm, be placed in 80 DEG C of air dry ovens dry 8 hours, then move into 130 DEG C of electric ovens dry 3.5 hours, with high speed disintegrator, after crossing 60 mesh sieves, obtain tannin albuminate finished product 2126g;
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (7)
1. prepare a tannin albuminate method with ovalbumin, it is characterized in that: stirred through centrifugal, adding distil water by the fresh albumen that eggshell adheres to, add tannic acid liquid and staticly settle, drying precipitate pulverizes and sieves, and obtains tannin albuminate.
2. the method preparing tannin albuminate according to claim 1, it is characterized in that, the method comprises the steps:
(1) preparation of protein liquid: the fresh albumen adhered to by eggshell, centrifugally throws away ovalbumin, adds water several times, constantly stirs and to obtain uniform protein liquid;
(2) preparation of tannic acid liquid: get Turkey-galls fragment, add water stirring, adds heat soaking, collects immersion liquid, immersion liquid filtered, leaves standstill, get supernatant liquor and namely obtain tannic acid liquid;
(3) synthesize: tannic acid liquid is added in protein liquid, leave standstill heating, precipitation, abandoning supernatant, wash, dry precipitation;
(4) dry: to get block after drying, make particle, dry, pulverize, sieve, obtain tannin albuminate finished product.
3. method according to claim 2, is characterized in that: in step (1), and the ratio of ovalbumin and water is: 1Kg:1.5 ~ 2.5L; Ovalbumin after water injection should at room temperature stir.
4. method according to claim 2, is characterized in that: in step (2), and the ratio of Turkey-galls fragment and water is 1Kg:8 ~ 12L and soaks 6 ~ 8 hours at 50 ~ 60 DEG C.
5. method according to claim 2, is characterized in that: in step (3), and ovalbumin and gallic weight ratio are 1:1.5 ~ 3.0.
6. method according to claim 2, is characterized in that: in step (3), and the temperature left standstill is: 55 ~ 65 DEG C; The time left standstill: 50 ~ 80 minutes; Water is 1:1 with the ratio of the precipitation that will wash.
7. method according to claim 2, is characterized in that: in step (4), when product carries out drying, first the drying 6 ~ 10 hours of 75 ~ 85 DEG C, then 125 ~ 135 DEG C of dryings 3 ~ 4 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410807822.0A CN104497131A (en) | 2014-12-23 | 2014-12-23 | Method for preparing tannalbin by using egg albumin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410807822.0A CN104497131A (en) | 2014-12-23 | 2014-12-23 | Method for preparing tannalbin by using egg albumin |
Publications (1)
| Publication Number | Publication Date |
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| CN104497131A true CN104497131A (en) | 2015-04-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410807822.0A Pending CN104497131A (en) | 2014-12-23 | 2014-12-23 | Method for preparing tannalbin by using egg albumin |
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| Country | Link |
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| CN (1) | CN104497131A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107125441A (en) * | 2016-02-29 | 2017-09-05 | 湖南晶天科技实业有限公司 | A kind of feed addictive tannalbin, a kind of composite feed additive and a kind of feed |
| CN108523133A (en) * | 2018-03-28 | 2018-09-14 | 广州富诺营养科技有限公司 | A kind of probiotics composite particles and preparation method thereof |
| CN112753862A (en) * | 2020-12-30 | 2021-05-07 | 五峰赤诚生物科技股份有限公司 | Preparation method of tannalbin for feed and prepared tannalbin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385918A (en) * | 2012-05-08 | 2013-11-13 | 陈�田 | Traditional Chinese medicine composition used for treating chronic enteritis, and application thereof |
-
2014
- 2014-12-23 CN CN201410807822.0A patent/CN104497131A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385918A (en) * | 2012-05-08 | 2013-11-13 | 陈�田 | Traditional Chinese medicine composition used for treating chronic enteritis, and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 余大方: "鞣酸蛋白生产工艺改进", 《中国药学杂志》 * |
| 李良铸、李明晔: "第5章多肽及蛋白质类药物", 《现代生化药物生产关键技术》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107125441A (en) * | 2016-02-29 | 2017-09-05 | 湖南晶天科技实业有限公司 | A kind of feed addictive tannalbin, a kind of composite feed additive and a kind of feed |
| CN108523133A (en) * | 2018-03-28 | 2018-09-14 | 广州富诺营养科技有限公司 | A kind of probiotics composite particles and preparation method thereof |
| CN112753862A (en) * | 2020-12-30 | 2021-05-07 | 五峰赤诚生物科技股份有限公司 | Preparation method of tannalbin for feed and prepared tannalbin |
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Application publication date: 20150408 |
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