CN104490948A - Pharmaceutical composition for treating chronic periodontitis and preparation method thereof - Google Patents
Pharmaceutical composition for treating chronic periodontitis and preparation method thereof Download PDFInfo
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- CN104490948A CN104490948A CN201410713896.8A CN201410713896A CN104490948A CN 104490948 A CN104490948 A CN 104490948A CN 201410713896 A CN201410713896 A CN 201410713896A CN 104490948 A CN104490948 A CN 104490948A
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- extract
- penthorum chinense
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7007—Drug-containing films, membranes or sheets
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a pharmaceutical composition for treating chronic periodontitis and a preparation method thereof. The pharmaceutical composition is prepared from an active ingredient and pharmaceutical excipients, wherein the active ingredient is a penthorum chinense pursh extract. The preparation method comprises the following steps: performing reflux extraction of penthorum chinense pursh with 55-70% ethanol solution by volume, and filtering to obtain an alcohol extract; performing reduced-pressure concentration to dryness to obtain a crude extract; adding water for dissolution, extracting with chloroform and separating to obtain a chloroform extract; and performing reduced-pressure concentration to obtain a thick paste, and drying to obtain the penthorum chinense pursh extract. The pharmaceutical composition has a remarkable curative effect on periodontitis, reduces the recurrence rate of a patient and causes few adverse reactions.
Description
Technical field
The invention belongs to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of pharmaceutical composition for the treatment of chronic periodontitis and preparation method thereof.
Background technology
Periodontitis is the destructive disease of chronic inflammatory disease, easily impacts patient's gingiva and Periodontal Supporting Tissue, causes loss of tooth.Chronic periodontitis pathogenic factor is a kind of oral disease that the Periodontal Supporting Tissue generation chronic infection caused due to microorganism in dental plaque causes.Microorganism in dental plaque and metabolite constantly act on the infringement that gingiva, periodontal membrane, cementum and alveolar bone etc. may cause certain irreversibility, affect masticatory function and the oral hygiene of patient.
At present, treatment periodontitis mainly adopts antibacterials, suppresses periodontal tissue and marginal gingiva breed bacteria.Clinical treatment periodontitis many employings topical therapeutic, remove the tartar existed above gingiva, remove the antibacterial mycin existed in periodontal pocket, and strike off the dentale of pathological changes in periodontal pocket, with this reduction of patient red swelling of gingiva, and improve patient's gingival hemorrhage and purulent discharge of pocket phenomenon.Although the bacterial plaque at brush surface can be removed by simple scaling, can not remove thoroughly antibacterial, along with breeding and the metabolism of antibacterial, can bacterial plaque be formed again, cause periodontitis to recur.After patient forms periodontal pocket, take medicine to process, in the past is mostly allow the oral extensive pedigree antibiotic of patient, but patient adopts the antibiotic of general, in oral cavity portion, blood levels is lower, be difficult to the effect producing sterilizing, meanwhile, long-term antibiotic effect can cause bacterial resistance, and then periodontitis is delayed repeatedly, can not fully recover.In addition, general medication can cause damage in various degree to liver, kidney, and side effect is larger.
Penthorum chinense (Penthorum chinense Pursh.), also known as Herba Lysimachiae Clethroids, mountain Monopterus albus (Zuiew), Radix Homonoiae Ripariae [Sichuan], Herba Penthori chinensis [Guizhou], for the herb of Crassulaceae penthorum plant Penthorum chinense Penthorum chinense Pursh, be in the news at present and there is diuretic dehumidifying, effect of stasis-dispelling and pain-killing, takes orally and is used for the treatment of jaundice, edema; Traumatic injury is controlled in external.At present, bibliographical information penthorum chinense pursh extract is not still had to have the biological activity for the treatment of periodontitis.
Summary of the invention
In view of the deficiencies in the prior art, the object of the invention is to by studying Chinese herbal medicine, thus pharmaceutical composition of a kind of effective treatment chronic periodontitis and preparation method thereof is provided.
In order to realize first object of the present invention, inventor, by lot of experiments research also persistent exploration, finally obtains following technical scheme:
Treat a pharmaceutical composition for chronic periodontitis, be prepared from by active component and pharmaceutic adjuvant, wherein said active component is penthorum chinense pursh extract, and described penthorum chinense pursh extract is the chloroform extract of Penthorum chinense ethanol extract.
Preferably, the pharmaceutical composition for the treatment of chronic periodontitis is external preparation as mentioned above.It should be noted that, the dry herb of Penthorum chinense raw material choose Crassulaceae penthorum plant Penthorum chinense Penthorum chinense Pursh of the present invention.
Second object of the present invention is the preparation method providing a kind of pharmaceutical composition according to above-mentioned treatment chronic periodontitis, wherein this technique comprises the steps: to be the alcoholic solution reflux, extract, of 55-70% by Penthorum chinense volumetric concentration, filters, obtains alcohol extract, be evaporated to dry, obtain crude extract, be dissolved in water, with chloroform extraction, separation obtains chloroform extraction liquid, concentrating under reduced pressure becomes thick paste, dry, obtains penthorum chinense pursh extract.
Preferably, the preparation method of the pharmaceutical composition for the treatment of chronic periodontitis as mentioned above, wherein said pharmaceutical composition is external preparation, and preferred external preparation is slow-release medicine-membrane.Medicine, as sustained release technology, is placed in medicine membrane support tissue by slow-release medicine-membrane, makes medicine can according to finite concentration release action.Therefore, penthorum chinense pursh extract is positioned in the tissue of medicine membrane support, penthorum chinense pursh extract according in the extraneous tissue of respective concentration release, can be allowed periodontal tissue be in certain microenvironment, effectively suppress dental plaque with this, evident in efficacy.
In order to study the clinical efficacy of penthorum chinense pursh extract slow-release medicine-membrane treatment periodontitis, the present inventor takes penthorum chinense pursh extract slow-release medicine-membrane to treat, wherein Penthorum chinense water extract is of no curative effect, the curative effect of ethanol extract is not obvious, and the chloroform extract of Penthorum chinense ethanol extract is evident in efficacy, can obviously improve patient clinical symptom, and by after treatment, two groups of patient's gingival index, gingival hemorrhage index, depth of pocket obviously improve.Therefore, the 3rd object of the present invention is to provide a kind of pharmaceutical applications, that is: the application of above-mentioned penthorum chinense pursh extract (chloroform extract of Penthorum chinense ethanol extract) in the medicine of preparation treatment chronic periodontitis.
The present invention is the slow-release medicine-membrane of external using penthorum chinense pursh extract as active fraction preparation, and its treatment periodontitis is evident in efficacy, reduces Patients on Recurrence rate, untoward reaction is few, and derives from plant due to it, belongs to natural product, therefore toxicity is little, and side effect is low, has more wide application prospect.
Detailed description of the invention
Be below concrete preparation example and the clinical practice example of penthorum chinense pursh extract involved in the present invention, technical scheme of the present invention is done and describes further, but protection scope of the present invention be not limited to this embodiment.
The preparation of embodiment 1 penthorum chinense pursh extract
Clean up and dry Penthorum chinense dry product 792g, be placed in the extraction element with round-bottomed flask, with the alcoholic solution reflux, extract, 3h that 8L volumetric concentration is 60%, four layers of filtered through gauze, obtain alcohol extract, repeat 2 times, merge alcohol extract, be evaporated to dry, crude extract, add 500mL purified water stirring and dissolving, with isopyknic chloroform extraction three times, separation obtains chloroform extraction liquid, decompression and solvent recovery is also condensed into thick paste, and drying baker is dried, and is ground into fine powder, cross 100 mesh sieves, obtain penthorum chinense pursh extract.
The preparation of embodiment 2 slow-release medicine-membrane
Preparation method: take penthorum chinense pursh extract fine powder prepared by embodiment 1, be dissolved in appropriate 95% alcoholic solution, for subsequent use as medicinal liquid; Take polyvinyl alcohol and the chitosan of recipe quantity, mixing, add 300ml water soaking, heating for dissolving, add the penthorum chinense pursh extract medicinal liquid of preparation, and add glycerol and tween 80, stir evenly, dry, 30min ultraviolet radiation disinfection, opens film.
Embodiment 3 penthorum chinense pursh extract is to the effectiveness study of chronic periodontitis
Meet chronic periodontitis 52 example of periodontitis diagnostic criteria, man 29 example, female 23 example; Age 25-67 year, year mean age (39.2 ± 5.3).Be divided into test group (26 example) and matched group (26 example) at random, two groups of data such as patient age, sex, without significant difference, have comparability.Two groups of patient's periodontals rinse through sodium chloride solution, take every wet process.Matched group patient takes iodine glycerol (Xiamen US business Pharmaceuticals Ltd, the accurate word H35021l17 of traditional Chinese medicines) to treat, 20g/ time, two days 1 time; Test group patient takes Chinese medicine extract slow-release medicine-membrane (preparing by the technique of embodiment 2) to insert in periodontal pocket, 1 piece/times, two days 1 time.Treat after 2 weeks and observe two groups of therapeutic outcomes, and by following canonical statistics curative effect:
Effective: clinical symptom disappearance, hemorrhage, pyorrhea, disappearance of swelling and ache, depth of pocket reduces degree more than 2mm, and gingival hemorrhage index is 0;
Effective: hemorrhage, pyorrhea, the symptom such as to swell and ache obviously are improved, and depth of pocket reduces degree more than 1mm;
Invalid: hemorrhage, pyorrhea, the clinical symptoms such as to swell and ache are not improved or increase the weight of, and depth of pocket does not reduce.
Can be found out by table 1, the clinical efficacy comparing result display of two groups of patients, test group total effective rate is 96.2%: matched group total effective rate is 69.2%, and treatment group clinical efficacy is better than matched group.
Table 1 liang group patient clinical Comparison of therapeutic [n (%)]
Can be found out by table 2, the gingival index of test group patient, gingival hemorrhage index, depth of pocket improve situation and are all better than matched group, and difference has statistical significance (P < 0.05 or P < 0.01).
Table 2 liang group patient gingival index, gingival hemorrhage index, depth of pocket contrast
▼compare with matched group, P < 0.05;
▼ ▼compare with matched group, P < 0.01.
Claims (4)
1. treat a pharmaceutical composition for chronic periodontitis, be prepared from, it is characterized in that by active component and pharmaceutic adjuvant, described active component is penthorum chinense pursh extract, and described penthorum chinense pursh extract is the chloroform extract of Penthorum chinense ethanol extract.
2. treat the pharmaceutical composition of chronic periodontitis according to claim 1, it is characterized in that, described pharmaceutical composition is external preparation.
3. a preparation method for penthorum chinense pursh extract according to claim 1, is characterized in that, the method comprises the steps: to be the alcoholic solution reflux, extract, of 55-70% by Penthorum chinense volumetric concentration, filter, obtain alcohol extract, be evaporated to dry, obtain crude extract, be dissolved in water, with chloroform extraction, be separated and obtain chloroform extraction liquid, concentrating under reduced pressure becomes thick paste, drying, obtains penthorum chinense pursh extract.
4. the application of penthorum chinense pursh extract according to claim 1 in the medicine of preparation treatment chronic periodontitis.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107362184A (en) * | 2017-08-04 | 2017-11-21 | 成都中医药大学 | The purposes of penthorum chinense pursh and its extract in the oral cavity medicine thing for the treatment of or/and prevention of gingivitis is prepared |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101732369A (en) * | 2008-11-06 | 2010-06-16 | 四川科伦新光医药有限公司 | Novel application of penthorum chinense pursh extract |
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101732369A (en) * | 2008-11-06 | 2010-06-16 | 四川科伦新光医药有限公司 | Novel application of penthorum chinense pursh extract |
Non-Patent Citations (3)
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刘锐: "复方奥硝唑/培氟沙星PLGA缓释微球的制备及对慢性牙周炎的药效学研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
姜明华: "赶黄草有效成分的分离鉴定及其活性与提取工艺研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
曹桦等: "离体快繁扯根菜总黄酮提取及抗菌活性研究", 《西华师范大学学报(自然科学版)》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107362184A (en) * | 2017-08-04 | 2017-11-21 | 成都中医药大学 | The purposes of penthorum chinense pursh and its extract in the oral cavity medicine thing for the treatment of or/and prevention of gingivitis is prepared |
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