CN104490940A - Biological extraction method of large algae active element - Google Patents
Biological extraction method of large algae active element Download PDFInfo
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- CN104490940A CN104490940A CN201510006265.7A CN201510006265A CN104490940A CN 104490940 A CN104490940 A CN 104490940A CN 201510006265 A CN201510006265 A CN 201510006265A CN 104490940 A CN104490940 A CN 104490940A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a biological extraction method of a large algae active element, and aims to solve the problems that the active components are easy to damage, the consumption of the extraction agent is high and the extraction efficiency is low of an extracting method in the prior art. A bionic extraction method of the large algae active element comprises the following steps: (1) preparing bionic gastric juice; (2) preparing bionic intestinal juice; (3) drying and crushing algae and screening the algae by a 50-80mesh screen to obtain uniform algae powder; (4) adding the bionic gastric juice into the algae powder and fermenting for 30-50 hours; (5) filtering the fermented medicine by using an ultra-filtration membrane with a hole diameter of 0.005-0.01 micron; and (6) adding the bionic intestinal juice into ultra-filtration permeate liquid, and adjusting pH to be 6.5-7.5. According to the large algae active element extracted by the method, the effective components are fully extracted, the absorption rate is high, and the structure or the composition of the effective components is unchanged. The large algae active element is important breakthrough in the field of application of natural medicines.
Description
Technical field
The present invention relates to fermentable extractive technique field, particularly a kind of biofermentation extracting method of tangleweed active extract.
Background technology
The complicated component of natural prodcuts, and plurality of active ingredients cooperatively interacts after entering human body, synergism, then plays the effect of disease therapy.Traditional purification & isolation technology, as decocting method, backflow, impregnating, and modern Chinese medicine extraction and separation technology, as superfine communication technique, supercritical extraction technique, adverse current dynamic extraction technology, absorption and purification technology, membrane separation technique etc., all continue to use purely traditional physics or chemical means, have nothing to do with the synergism of the Effective Component of Chinese Medicine being absorbed into human body, the structure of destructible Effective Component of Chinese Medicine or composition, be difficult to the effective ingredient contained in Chinese medicine to extract completely, and extractant consumption large, need repeatedly extract, extraction efficiency is low, the cycle is long.
Bionic extraction method is the new method proposed in recent years, and it is from biopharmaceutical angle, is combined by overall medicine organon with molecular medicine organon, the environment that simulation oral drugs absorb through gastrointestinal transit, adopts the guiding partition method under active guidance.The method can not only give full play to the comprehensive function of Traditional Chinese drug mixture, and monomer component can be utilized again to control the quality of Chinese medicine preparation, and loss of effective components is few.But the bionical liquid used in existing bionic extraction technology is only the pH value imitating gastric juice or intestinal juice, and bionical degree is low, and the extraction efficiency of effective ingredient and the loss of effective ingredient still do not have clear improvement.
Such as, the Chinese invention patent that publication No. is " CN102266370 " discloses the semi-bionic extraction method of anti-gastric cancer active substance in a kind of Flos Chrysanthemi, comprise the following steps: after 1) being pulverized by the Flos Chrysanthemi of drying, cross 40-60 mesh sieve, then add the buffer I that PH is 2.0-2.2, be placed in 36.5-37.5 DEG C of water-bath and extract 1-2 hour; Extraction terminates rear filtration, discards filtrate I; 2) in step 1) gained filtering residue, add the buffer II that PH is 7.8-8.2, be placed in 36.5-37.5 DEG C of water-bath and extract 1-2 hour; After extraction terminates, filter, go filtrate II; The process of filtrate II drying, obtains Flos Chrysanthemi anticancer extract.
Tangleweed active extract is that human or animal is decomposed after segmentation again enters duodenum, intestinal absorption by Gastric juice digestion and arrives liver by portal vein, arrives focus work after liver decomposes by blood targeting.At present still not efficient and to effective ingredient harmless tangleweed active extract extracting method.
Summary of the invention
The object of the present invention is to provide a kind of biological extraction process of tangleweed active extract, to solve the tangleweed active extract extracting method of prior art, the structure of destructible Sargassum effective ingredient or composition, be difficult to the effective ingredient contained in Sargassum to extract completely, and extractant consumption large, need repeatedly extract, extraction efficiency is low, the cycle is long problem.
Technical scheme of the present invention is achieved in that
A biological extraction process for tangleweed active extract, comprises the following steps:
1) bionical gastric juice is prepared:
A. getting concentration is 10
7-10
8the lactobacillus bacterium of cfu/ml and concentration are 10
6-10
7the yeast of cfu/ml by volume 3:1 mixes;
B. in mixed bacteria liquid, add the dilute hydrochloric acid that concentration is 3mol/L, regulate ph value of mixture to 0.9-1.5;
C. add sodium chloride saturated solution, regulate solution density to 1.006-1.009g/ml, to ensure that the osmotic pressure of described bionical gastric juice is equal to human gastric juice;
D. add feed with balanced nutrients, in feed with balanced nutrients, the concentration of Rhizoma Dioscoreae powder and xanthan gum is 0.5-2g/100ml;
E. add pepsin, concentration is 0.5-2g/100ml;
F. add stable regulation agent, wherein sodium citrate concentration is 1-3g/100ml, and concentration of glucose is 2-4g/100ml;
2) bionical intestinal juice is prepared:
A. getting concentration is 5 × 10
6-10
7the bacillus cereus bacterium liquid of cfu/ml;
B. in bacillus cereus bacterium liquid, high concentration K is added
2pO
4solution, makes the K in mixed liquor
2pO
4concentration be 0.8-1.5g/100ml;
C. add the pancreatin aqueous solution that concentration is 5%, the concentration being adjusted to pancreatin is 0.25-0.5%;
D. PH to 8-10 is regulated with the sodium hydroxide solution that concentration is 0.1-0.3mol/L;
3) tangleweed drying and crushing is also crossed 50-80 mesh sieve;
4) after sieving, marine algae powder adds above-mentioned bionical gastric juice, and make bionical gastric juice submergence marine algae powder, then ferment 20-50h;
5) medicine after fermentation adopts aperture to be the ultrafiltration membrance filter of 0.005-0.01um;
6) in ultrafiltrate, add bionical intestinal juice, adjustment PH is 6.5-7.5, obtains tangleweed active extract extract.
In the biological extraction process of tangleweed active extract of the present invention:
A bacterium implants culture medium, 37 DEG C, cultivate 20-40h under anaerobic condition, obtain A bacterium bacterium liquid.
A bacterium culture medium: casein peptone 10.0g, Carnis Bovis seu Bubali cream 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamidogen 2.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, MgSO4.7H2O 0.2g, MnSO4.H2O 0.05g, CaCO3 20.0g, agar 15.0g, distilled water 1.0L, pH6.8.
Saccharomyces cerevisiae implants culture medium, 25-28 DEG C, cultivate 20-40h under anaerobic condition, obtain saccharomyces cerevisiae bacterium liquid.
Saccharomyces cerevisiae bacterium culture medium: 5 ° of B é beerwort 1.0L, agar 15.0g.
Basophilia bacillus subtilis implants culture medium, 28-28 DEG C, cultivate 20-40h under aerobic conditions, obtain Bacillus alcalophilus bacterium liquid.
Bacillus subtilis bacterium culture medium: 0.5 gram, sodium chloride, 2 grams, agar, Carnis Bovis seu Bubali cream 1 gram, peptone 1 gram, 100 milliliters, water, PH 7.2.
Beneficial effect of the present invention is:
The biological extraction process of tangleweed active extract of the present invention, after Sargassum generality being pulverized, carries out the fermentation of bionics biological activity with bionical gastric juice to Sargassum powder, then adopts membrane separation process to carry out the extraction of tangleweed active extract.Then adopt bionical intestinal juice that the PH of extracted tangleweed active extract solution is regulated 6.5-7.5, make active extract solution be alkalescence, neutrality or faintly acid, be applicable to taking, bionical intestinal juice regulates PH simultaneously, can improve the infiltration rate of tangleweed active extract.
Do not use in the biological extraction process of tangleweed active extract of the present invention and produce destructive means to the effective ingredient in Sargassum, structure or the composition of the tangleweed active extract effective ingredient extracted are constant; The extraction ratio of the inventive method to the tangleweed active extract of Sargassum is high, in addition, the tangleweed active extract adopting the biological extraction process of tangleweed active extract of the present invention to extract does not need stomach, intestinal juice digest and decompose, directly can enter focus, improves the infiltration rate of Effective Component of Chinese Medicine.
The present invention is further illustrated below in conjunction with a clinical case:
Week certain, female, 46 years old, people from Anqiu, Shandong, first visit in October, 2013, vague epigastralgia 2 years, stomach did not relax, and within nearly two months, increases the weight of, and the warm pain relieved by pressing of stomachache happiness, occasionally has singultus, without having loose bowels.Lethargy, extremity are afraid of cold, and happiness heat is disliked cool, and bipod is afraid of cold and is increased the weight of in bed.Blood pressure (105/60mmHg) on the low side usually, often feels dizzy, dim eyesight.In vain thin in the middle part of the light red tongue of body of the tongue, while there is indentation, left cun closes stringy and thready pulse, and right GUAN-pulse being deep string is slightly weak.Microscopy finding: gastric mucosa is coarse, congested, body of stomach pleat marshalling, mucosa diffusivity congestion and edema, the erosion of intensive speckle sample has petechia, and stomach corner shape normal mucosa is congested, antrum diffusivity congestion and edema, is dispersed in patch sample rotten to the corn, pylorus mucous hyperemia.Conclusion: non-atrophic gastritis.
Adopt the Drug therapy that the biological extraction process of tangleweed active extract of the present invention is obtained, extract through extracting method of the present invention and obtain micro-yellow tangleweed active extract liquid, each 50ml, takes three times on 1st.After taking, namely pain is taken a favourable turn, and takes 10 days altogether, and later patient's readme without stomachache so far.
Points for attention: when applying this biologics, jointly must not use with other antibiotic.
Accompanying drawing explanation
Fig. 1 adopts the biological extraction process preparation of tangleweed active extract of the present invention containing the process chart of tangleweed active extract medicine.
Detailed description of the invention
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The strain used in the biological extraction process of tangleweed active extract of the present invention is provided by Chinese food industrial fermentation academy.
Embodiment 1
The biological extraction process of tangleweed active extract of the present invention comprises the following steps:
(1) bionical gastric juice is prepared:
Get 600mlA bacterium liquid and the mixing of 200ml saccharomyces cerevisiae bacterium liquid, get the dilute hydrochloric acid that concentration is 3mol/L, regulate the PH to 1.3 of mixed bacteria liquid, add solid sodium chloride, regulate the density of solution to 1.006-1.009g/ml, add 8g Rhizoma Dioscoreae powder, add 8g xanthan gum, Rhizoma Dioscoreae powder and xanthan gum are as nutrition regulating agent, add 8g pepsin, add 16g sodium citrate, add 24g glucose, sodium citrate and glucose, as stable regulation agent, obtain bionical gastric juice.
(2) bionical intestinal juice is prepared
Getting Bacillus alcalophilus concentration is 5 × 10
6-10
7the bacterium liquid 500ml of cfu/ml, adds 6g potassium dihydrogen phosphate solid and dissolves, then join in bacterium liquid.Get 3.0g pancreatin, add 300ml water and make it to dissolve.The mixed bacteria liquid of gained of step being gone on the market mixes with trypsin solution, and is diluted to 1000ml.
(3) get 1kg Sargassum powder, in Chinese medicinal powder, add bionical gastric juice, bionical gastric juice submergence Chinese medicinal powder, fermentation 36h.After having fermented, the medicine after fermentation is adopted the ultrafiltration membrance filter of 0.005um.After ultrafiltration membrance filter gained permeate in add bionical intestinal juice, adopt bionical intestinal juice regulate PH to 7, obtain tangleweed active extract extract.Oral liquid complex preparation is added, the obtained oral liquid containing tangleweed active extract in tangleweed active extract extract.
Embodiment 2
(1) bionical gastric juice is prepared:
Get 900mlA bacterium liquid and the mixing of 300ml saccharomyces cerevisiae bacterium liquid, get the dilute hydrochloric acid that concentration is 3mol/L, regulate the PH to 1.0 of mixed bacteria liquid, add solid sodium chloride, regulate the density of solution to 1.008g/ml, add 18g Rhizoma Dioscoreae powder, add 18g xanthan gum, Rhizoma Dioscoreae powder and xanthan gum are as nutrition regulating agent, add 18g pepsin, add 32g sodium citrate, add 32g glucose, sodium citrate and glucose, as stable regulation agent, obtain bionical gastric juice.
(2) bionical intestinal juice is prepared
Getting Bacillus alcalophilus concentration is 5 × 10
6-10
7the bacterium liquid 500ml of cfu/ml, adds 6g potassium dihydrogen phosphate solid and dissolves, then join in bacterium liquid.Get 5.0g pancreatin, add 300ml water and make it to dissolve.The mixed bacteria liquid of gained of step being gone on the market mixes with trypsin solution, and is diluted to 1000ml.
(3) get 1kg Sargassum powder, in Chinese medicinal powder, add bionical gastric juice, bionical gastric juice submergence Chinese medicinal powder, fermentation 48h.After having fermented, the medicine after fermentation is adopted the ultrafiltration membrance filter of 0.01um.After ultrafiltration membrance filter gained permeate in add bionical intestinal juice, adopt bionical intestinal juice regulate PH to 7, obtain tangleweed active extract extract.Oral liquid complex preparation is added, the obtained oral liquid containing tangleweed active extract in tangleweed active extract extract.Obtained fermentation is contained tangleweed active extract oral liquid concentrate through negative pressure and carry out micronizing after drying, make capsule preparations, must containing the capsule of tangleweed active extract.
Embodiment 3
Get 900mlA bacterium liquid and the mixing of 300ml saccharomyces cerevisiae bacterium liquid, get the dilute hydrochloric acid that concentration is 3mol/L, regulate the PH to 1.5 of mixed bacteria liquid, add solid sodium chloride, regulate the density of solution to 1.006g/ml, add 24g Rhizoma Dioscoreae powder, add 24g xanthan gum, Rhizoma Dioscoreae powder and xanthan gum are as nutrition regulating agent, add 18g pepsin, add 32g sodium citrate, add 48g glucose, sodium citrate and glucose, as stable regulation agent, obtain bionical gastric juice.Getting Bacillus alcalophilus concentration is 5 × 10
6-10
7the bacterium liquid 500ml of cfu/ml, adds 6g potassium dihydrogen phosphate solid and dissolves, then join in bacterium liquid.Get 5.0g pancreatin, add 300ml water and make it to dissolve.The mixed bacteria liquid of gained of step being gone on the market mixes with trypsin solution, and is diluted to 1000ml.Get 1kg Sargassum powder, in Chinese medicinal powder, add bionical gastric juice, bionical gastric juice submergence Chinese medicinal powder, fermentation 36h.After having fermented, the medicine after fermentation is adopted the ultrafiltration membrance filter of 0.005um.After ultrafiltration membrance filter gained permeate in add bionical intestinal juice, adopt bionical intestinal juice regulate PH to 7, obtain tangleweed active extract extract.Oral liquid complex preparation is added, the obtained oral liquid containing tangleweed active extract in tangleweed active extract extract.Obtained fermentation is contained tangleweed active extract oral liquid concentrate through negative pressure and carry out micronizing after drying, make capsule preparations, must containing the capsule of tangleweed active extract.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. a biological extraction process for tangleweed active extract, is characterized in that, comprises the following steps:
1) bionical gastric juice is prepared:
A. getting concentration is 10
7-10
8the lactobacillus bacterium of cfu/ml and concentration are 10
6-10
7the yeast of cfu/ml by volume 3:1 mixes;
B. in mixed bacteria liquid, add the dilute hydrochloric acid that concentration is 3mol/L, regulate ph value of mixture to 0.9-1.5;
C. add sodium chloride saturated solution, regulate solution density to 1.006-1.009g/ml, to ensure that the osmotic pressure of described bionical gastric juice is equal to human gastric juice;
D. add feed with balanced nutrients, in feed with balanced nutrients, the concentration of Rhizoma Dioscoreae powder and xanthan gum is 0.5-2g/100ml;
E. add pepsin, concentration is 0.5-2g/100ml;
F. add stable regulation agent, wherein sodium citrate concentration is 1-3g/100ml, and concentration of glucose is 2-4g/100ml;
2) bionical intestinal juice is prepared:
A. getting concentration is 5 × 10
6-10
7the bacillus cereus bacterium liquid of cfu/ml;
B. in bacillus cereus bacterium liquid, K is added
2pO
4saturated solution makes the K in mixed liquor
2pO
4concentration is 0.5-1.2g/100ml;
C. add the pancreatin aqueous solution that concentration is 5%, the concentration being adjusted to pancreatin is 0.25-0.5%;
D. PH to 8-10 is regulated with the sodium hydroxide solution that concentration is 0.1-0.3mol/L;
3) tangleweed drying and crushing is also crossed 50-80 mesh sieve;
4) after sieving, marine algae powder adds above-mentioned bionical gastric juice, and make bionical gastric juice submergence marine algae powder, then ferment 20-50h;
5) medicine after fermentation adopts aperture to be the ultrafiltration membrance filter of 0.005-0.01um;
6) in ultrafiltrate, add bionical intestinal juice, adjustment PH is 6.5-7.5, obtains tangleweed active extract extract.
2. the biological extraction process of tangleweed active extract according to claim 1, is characterized in that: described lactobacillus is for opening A bacterium, and described yeast is saccharomyces cerevisiae.
3. the biological extraction process of tangleweed active extract according to claim 1, is characterized in that: in described bionical gastric juice, the concentration of Rhizoma Dioscoreae powder and xanthan gum is 0.5-2g/100ml, pepsic concentration is 0.5-2g/100ml, the concentration of sodium citrate is 1-3g/100ml, the concentration of described glucose is 2-4g/100ml.
4. the biological extraction process of tangleweed active extract according to claim 1, is characterized in that: described bacillus cereus is basophilia bacillus subtilis.
5. the biological extraction process of tangleweed active extract according to claim 1, is characterized in that: K in described bionical intestinal juice
2pO
4concentration be 0.5-1.2g/100ml, the concentration of pancreatin is 0.25-0.5g/100ml.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907145A (en) * | 2019-04-24 | 2019-06-21 | 湖南省定生保健品经营有限公司 | The preparation method and application of tea therapy drink |
| CN110215422A (en) * | 2019-05-28 | 2019-09-10 | 青春妆园生物科技(广州)有限公司 | A kind of allicin shines new flesh water of face moisturizing and preparation method thereof |
| CN110354015A (en) * | 2019-07-04 | 2019-10-22 | 沈衡平 | A kind of allicin drives face maintenance essence aqueous and preparation method thereof |
| CN110354016A (en) * | 2019-07-04 | 2019-10-22 | 沈衡平 | A kind of bright fair-skinned light sensation essence aqueous of allicin and preparation method thereof |
-
2015
- 2015-01-07 CN CN201510006265.7A patent/CN104490940A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907145A (en) * | 2019-04-24 | 2019-06-21 | 湖南省定生保健品经营有限公司 | The preparation method and application of tea therapy drink |
| CN110215422A (en) * | 2019-05-28 | 2019-09-10 | 青春妆园生物科技(广州)有限公司 | A kind of allicin shines new flesh water of face moisturizing and preparation method thereof |
| CN110354015A (en) * | 2019-07-04 | 2019-10-22 | 沈衡平 | A kind of allicin drives face maintenance essence aqueous and preparation method thereof |
| CN110354016A (en) * | 2019-07-04 | 2019-10-22 | 沈衡平 | A kind of bright fair-skinned light sensation essence aqueous of allicin and preparation method thereof |
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