CN104483359A - Glucose sensor - Google Patents
Glucose sensor Download PDFInfo
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- CN104483359A CN104483359A CN201410723879.2A CN201410723879A CN104483359A CN 104483359 A CN104483359 A CN 104483359A CN 201410723879 A CN201410723879 A CN 201410723879A CN 104483359 A CN104483359 A CN 104483359A
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Abstract
The invention provides a glucose sensor which comprises a sensor base material, an insulating medium and a lead, wherein the sensor base material comprises a reference electrode, a working electrode and a counter electrode which are insulated with one another by the insulating medium. The glucose sensor disclosed by the invention has the excellent glucose detection accuracy and specificity, and has the very good stability in vitro and in vivo.
Description
Technical field
The present invention relates to field of electrochemical detection, be specifically related to a kind of glucose sensor.
Background technology
Along with the raising of people's living standard, the change of dietary structure and living-pattern preservation, the increase of aging population and fat incidence, the incidence of disease of diabetes is in ascendant trend year by year.The global frequently-occurring disease of diabetic history, whole world diabetic more than 3.5 hundred million, the health of the serious harm mankind.Continuous blood sugar monitoring to the significance of diabetes diagnosis and treatment more and more be familiar with by people.If the monitoring of painless and continual continuous blood sugar can be carried out to patient, more close blood sugar concentration continually varying data are provided, just can reflect the blood sugar " overall picture " of patient, measure those uncared-for blood glucose information, disclose hiding blood sugar condition, find the peak value etc. of the hypoglycemic episodes repeatedly of those no conscious symptons, dawn phenomenon and hyperglycaemia, for drafting glucose-lowering treatment scheme that is more reasonable, individuation, valuable clinical foundation is provided, thus instructs the treatment of diabetes better.
The ultimate principle of current blood glucose sensor is, in the presence of glucose oxidase, glucose oxidase is gluconic acid and hydrogen peroxide by oxygen, and the signal intensity then produced by hydrogen peroxide and electrode generation electrochemical reaction calculates the content of glucose.
Although the selectivity of the enzyme reaction of this kind of sensor is very strong, but still have many endogenic electroactive substances, i.e. chaff interference, detection is had an impact, as salicylic acid, uric acid, paracetamol etc. are easily oxidized under the operating voltage of sensor, thus equally produce electric signal with the hydrogen peroxide that enzymatic produces, therefore can produce error.Wherein a kind of effective strategy be exactly electrode surface cover a layer-selective through film to reduce chaff interference through, many different macromolecules, various hybrid films or monofilm, the film selected based on size Selection or polarity has all been introduced into glucose sensor, in order to get rid of different electroactive substances.Selectivity is very effective through film, but can not eliminate interference completely, and in general, some charged chaff interferences, as salicylic acid and uric acid, are easily eliminated through film by selectivity, but the Small molecular of some neutrality, as paracetamol, be then difficult to eliminate.
Summary of the invention
An object of the present invention is to provide a kind of glucose sensor, and structure of the present invention is simple, easily manufactured, measures accurately.
The invention provides a kind of glucose sensor, comprising: sensor substrate, insulating medium and wire, described sensor substrate comprises contrast electrode, working electrode and to electrode, described contrast electrode, working electrode, to adopting insulating medium to insulate between electrode.
Described working electrode surface is provided with enzyme layer, and described enzyme layer surface is provided with anti-interference layer.
Described insulating medium is selected from Polyvinylchloride, polycarbonate, the one in polystyrene.
Described working electrode is platinum plate electrode, and described is platinum electrode to electrode, and described contrast electrode is silver chloride electrode.
Described anti-interference layer is acetylbutyrylcellulose.
Described enzyme layer is glucose oxidase layer, is provided with bonding coat between described enzyme layer and described anti-interference layer.
Beneficial effect of the present invention is, uses a kind of glucose sensor provided by the invention.This sensor electrode surface is provided with polymeric membrane, and described polymeric membrane comprises anti-interference layer.Anti-interference layer provided by the present invention can hinder the electroactive chaff interferences such as paracetamol, uric acid, ascorbic acid, salicylic acid through, can make simultaneously hydrogen peroxide through, thus make glucose sensor provided by the present invention have excellent glucose detection accuracy and specificity, and in vitro and in vivo all there is good stability.
Accompanying drawing explanation
Fig. 1 is shown as the structural drawing of glucose sensor of the present invention.
Fig. 2 is shown as the vertical view of glucose sensor of the present invention.
Embodiment
Hereafter will describe embodiments of the invention in detail by reference to the accompanying drawings.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.In the accompanying drawing of following embodiment, the identical label that each accompanying drawing occurs represents identical feature or parts, can be applicable in different embodiment.
As shown in Figures 1 and 2, an object of the present invention is to provide a kind of glucose sensor, and structure of the present invention is simple, easily manufactured, measures accurately.
The invention provides a kind of glucose sensor, comprise: sensor substrate 1, insulating medium 6 and wire 5, described sensor substrate 1 comprises contrast electrode 2, working electrode 3 and to electrode 4, described contrast electrode 2, working electrode 3, to adopting insulating medium 6 to insulate between electrode 4.
Described working electrode surface is provided with enzyme layer 7, and described enzyme layer 7 surface is provided with anti-interference layer 8.
Described contrast electrode 2, working electrode 3, to adopting insulating medium 6 to insulate between electrode 4, described insulating medium 6 is selected from Polyvinylchloride, polycarbonate, the one in polystyrene.
Described working electrode 3 is platinum plate electrode, and described is platinum electrode to electrode 4, and described contrast electrode 2 is silver chloride electrode.
Described anti-interference layer 8 is acetylbutyrylcellulose.
Described enzyme layer 7 is glucose oxidase layer, is provided with bonding coat between described enzyme layer 7 and described anti-interference layer 8.
Preferably, the material of described bonding coat is selected from polyvinylpyrrolidone (PVP), aminopropyl triethyl siloxane (APTES) or silicone adhesive (such as main body is the dimethylsiloxane polymer containing active function groups); Preferred thickness is 1-5 μm.
The mean molecular weight of described polyvinylpyrrolidone (PVP) is 100000-5000000.
In the present invention, selected bonding coat also has the effect improving adjacent film layers affinity, and that is, when adjacent film layers affinity is enough, such as, anti-interference layer material contains some groups being conducive to enzyme layer absorption, can not use extra bonding coat.And those skilled in the art also according to the character of the anti-interference layer of difference and bonding coat, can regulate the bonding coat obtaining suitable thickness.
Rete of the present invention can pass through dip-coating, brushes, and is coated with zona coating, and the multiple method such as spin coating and spraying modifies electrode or the anti-interference layer surface of glucose sensor.
A kind of glucose sensor electrode surface provided by the invention is provided with polymeric membrane, and described polymeric membrane comprises anti-interference layer.Anti-interference layer provided by the present invention can hinder the electroactive chaff interferences such as paracetamol, uric acid, ascorbic acid, salicylic acid through, can make simultaneously hydrogen peroxide through, thus make glucose sensor provided by the present invention have excellent glucose detection accuracy and specificity, and in vitro and in vivo all there is good stability.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (6)
1. a glucose sensor, it is characterized in that, comprise: sensor substrate, insulating medium and wire, described sensor substrate comprises contrast electrode, working electrode and to electrode, described contrast electrode, working electrode, to adopting insulating medium to insulate between electrode.
2. a kind of glucose sensor as claimed in claim 1, is characterized in that, described working electrode surface is provided with enzyme layer, and described enzyme layer surface is provided with anti-interference layer.
3. a kind of glucose sensor as claimed in claim 1, is characterized in that, described insulating medium is selected from Polyvinylchloride, polycarbonate, the one in polystyrene.
4. a kind of glucose sensor as claimed in claim 2, is characterized in that, described working electrode is platinum plate electrode, and described is platinum electrode to electrode, and described contrast electrode is silver chloride electrode.
5. a kind of glucose sensor as claimed in claim 3, is characterized in that, described anti-interference layer is acetylbutyrylcellulose.
6. a kind of glucose sensor as claimed in claim 3, is characterized in that, described enzyme layer is glucose oxidase layer, is provided with bonding coat between described enzyme layer and described anti-interference layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410723879.2A CN104483359A (en) | 2014-12-03 | 2014-12-03 | Glucose sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410723879.2A CN104483359A (en) | 2014-12-03 | 2014-12-03 | Glucose sensor |
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| CN104483359A true CN104483359A (en) | 2015-04-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410723879.2A Pending CN104483359A (en) | 2014-12-03 | 2014-12-03 | Glucose sensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109863403A (en) * | 2016-10-13 | 2019-06-07 | 威里利生命科学有限责任公司 | Disposable glucose biological sensor including activity sensor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301232A (en) * | 2008-12-24 | 2011-12-28 | 爱德华兹生命科学公司 | Membrane layer for electrochemical biosensor and method of accommodating electromagnetic and radiofrequency fields |
| CN103487486A (en) * | 2013-09-22 | 2014-01-01 | 上海移宇科技有限公司 | Glucose sensor and preparation method thereof |
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2014
- 2014-12-03 CN CN201410723879.2A patent/CN104483359A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301232A (en) * | 2008-12-24 | 2011-12-28 | 爱德华兹生命科学公司 | Membrane layer for electrochemical biosensor and method of accommodating electromagnetic and radiofrequency fields |
| CN103487486A (en) * | 2013-09-22 | 2014-01-01 | 上海移宇科技有限公司 | Glucose sensor and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109863403A (en) * | 2016-10-13 | 2019-06-07 | 威里利生命科学有限责任公司 | Disposable glucose biological sensor including activity sensor |
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Application publication date: 20150401 |
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