CN104480079B - A kind of Cyanea capillata superoxide dismutase and its encoding gene and application - Google Patents
A kind of Cyanea capillata superoxide dismutase and its encoding gene and application Download PDFInfo
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Abstract
The invention belongs to biomedicine technical field, the relevant research report to jellyfish superoxide dismutase is had not yet to see.The invention provides a kind of Cyanea capillata superoxide dismutase and preparation method thereof, described Cyanea capillata superoxide dismutase, with such as SEQ ID NO:The protein of amino acid sequence composition shown in 2.Present invention also offers the encoding gene of Cyanea capillata superoxide dismutase, with such as SEQ ID NO:Nucleotide sequence shown in 1.Meanwhile, present invention also offers the application of Cyanea capillata superoxide dismutase and its encoding gene in anti-oxidation medicine, antiradiation drug, shielding medicine for skin, food preservative, antiaging agent etc. is prepared.
Description
Technical field
The invention belongs to biomedicine technical field, and in particular to a kind of Cyanea capillata superoxide dismutase and its volume
Code gene and application.
Background technology
Medusa Cnidaria, is that a class is widely distributed, biological total amount very huge halomereid.It is existing big
Quantity research shows, is rich in various bioactivators in medusoma, including medusocongestin albumen and some activity it is strong, with good
The New function albumen of good DEVELOPMENT PROSPECT.Jellyfish class is swum in 4-6 meters of deep seawater surfaces, and strong light radiation ring is lived in for a long time
Under border.Oxidative damage is that ultraviolet radioactive causes one of important mechanisms of body injury, and there are some researches show planktonic organism is to avoid
Or the infringement that causes of ultraviolet radioactive is reduced, selected by long adaptation, generate that species is various, structure is special in its body
Different, active strong anti-oxidation active substance.Flourishing antioxidant system is protecting cell and organism from light and photosensitive color
Played an important role in plain equivalent damage, radiation injury of the strong light ultraviolet to organism can be protected, remove what is produced in oxidation reaction
Free radical.Studies have found that, Cyanea capillata tentacle extract has very strong antioxidation activity in vitro, to active chalcogen
Scavenging activity is strong more than conventional antioxidant vitamin C.By the methods such as ammonium sulfate precipitation, gel permeation chromatography also respectively from
Isolated in jellyfish Rhopilema esculentum, Stomolophus meleagris a variety of with substantially removing free radical
The albumen of function.These results show that medusoma includes active strong antioxidation activity component, are anti-oxidant, anti-spokes
Penetrate the important sources of active material.Nevertheless, at present to the constituting of antioxidant system, important antioxidant reductase in medusoma
Sequence information, expression, the antioxidant activity level of (albumen) etc. are not yet systematic to be understood.
Superoxide dismutase (SOD) is that to be widely present in prokaryotes important anti-oxidant with a class in eucaryote
Enzyme, can protect these biologies from ultra-oxygen anion free radical (O2 -) caused by oxidative damage, be organism lives in its metabolism
Indispensable important protective enzyme in dynamic.Superoxide dismutase belongs to metal desmoenzyme, can specifically be catalyzed superoxide anion
Disproportionated reaction, generation hydrogen peroxide and water occur for free radical, and are considered as the main effect enzyme for playing this function.Its is anti-
Answer process as follows：
2O2 -+2H+=H2O2+O2
The hydrogen peroxide produced in reaction can be removed further by catalase and peroxidase, be converted into water and oxygen
Molecule.Organism protects ultra-oxygen anion free radical that itself aerobic cell produces from normal metabolic processes by the reaction
Poison, so that cell keeps its normal vitality.In addition, superoxide dismutase is also found to significantly carry
High body is to bacterium and the immunity and the resistance to illeffects such as toxic chemical exposure, thermal stimulus of virus infection.
The difference of metal prothetic group according to needed for playing active function, superoxide dismutase can be divided into four types：(1) the super of manganese is needed
Superoxide dismutase Mn-SOD (Sod A)；(2) the superoxide dismutase Fe-SOD (Sod B) of iron is needed；(3) need copper and
The superoxide dismutase Cu/Zn SOD (Sod C) of zinc；(4) the superoxide dismutase Ni-SOD (Sod N) of nickel is needed.It is super
Superoxide dismutase is described as " street cleaner " of biological internal rubbish, is clinically mainly used in treatment oxygen poisoning, senile white
A variety of diseases such as cataract or glaucoma, diabetes, angiocardiopathy；Superoxide dismutase is alternatively arranged as antiradiation agent, for adjuvant radiotherapy,
To reduce the side effect of high dose radiation；Superoxide dismutase is also widely used in daily chemical industry, with the addition of super oxygen
The skin care item of compound mutase have obvious resisting age of skin, go the functions such as foxiness.In view of sending out shape in above-mentioned effect, the present invention
The superoxide dismutase of jellyfish original possesses significant Development volue, in anti-oxidant and antiradiation drug, cosmetics, food
Product, agricultural have huge application potential and wide market prospects with chemical industry.
Seminar where the present invention is directed to the extraction and research of active component in medusoma, promulgated by the State Council in having obtained always
Bright patent ZL201310189933.5, entitled " a kind of Cyanea capillata thioredoxin and its encoding gene are with answering
With ", application publication number：CN103232979A；Chinese patent CN201310188702.2, entitled " one kind hair are applied for
Shape rosy clouds jellyfish peroxiredoxin and its encoding gene and application ", application publication number：CN103255113A；
CN201310258184.7, entitled " a kind of Cyanea capillata astacin sample metalloproteinases CALP1 and its encoding gene
With expression "；CN201310629343.X, entitled " a kind of preparation method of jellyfish cardiovascular toxin crude extract ",
Application publication number：CN103613653A；CN201310628068.X, it is entitled " a kind of jellyfish hematoxin crude extract
Preparation method ", application publication number：CN103626861A etc..
At present, both at home and abroad there is not yet the relevant research to jellyfish superoxide dismutase is reported.
The content of the invention
It is of the invention it is an object of the invention to provide a kind of Cyanea capillata superoxide dismutase and its encoding gene
Another object be provide the Cyanea capillata superoxide dismutase prepare anti-oxidation medicine, antiradiation drug, skin prevent
The application protected in agent, food preservative, antiaging agent etc..
The present invention is carried out by building Cyanea capillata tentacle tissue cDNA library, and to the sequence of the library recombinant clone
Determine and annotation analysis, obtain the encoding gene of Cyanea capillata superoxide dismutase.The Cyanea capillata superoxides
Mutase is the jellyfish Like superoxide dismutase with obvious antioxidation activity found first, is anti-oxidant system in medusoma
The important activity component of system, the albumen has a good application prospect in anti-oxidant, antiradiation drug research.
The main technical schemes of the present invention are, by building Cyanea capillata tentacle tissue cDNA library, it to be surveyed
Sequence and screening, obtain the encoding gene of Cyanea capillata superoxide dismutase, and its antioxidation activity is studied.
There is provided a kind of Cyanea capillata superoxide dismutase, a kind of described hair shape rosy clouds for the first aspect of the present invention
Jellyfish superoxide dismutase, the protein with following (I) or (II)：
(ⅰ)SEQ ID NO:The protein of amino acid sequence composition shown in 2；
(ⅱ)SEQ ID NO:Amino acid sequence shown in 2 is substituted, lacks and/or added one or several amino acid and same
Etc. function as derived from (I) protein.
A kind of described Cyanea capillata superoxide dismutase, such as SEQ ID NO:Amino acid sequence shown in 2, the egg
Signal peptide is free of in vain, is non-secreted protein, is positioned at cytoplasm, molecular weight is 16.0kDa, and isoelectric point is 6.2.
It is following (I) or (II) present invention also offers a kind of encoding gene of Cyanea capillata superoxide dismutase
DNA molecular：
(ⅰ)SEQ ID NO:Nucleotide sequence shown in 1；
(II) and SEQ ID NO:Nucleotide sequence of the nucleotide sequence homology more than 80% shown in 1.
A kind of described Cyanea capillata superoxide dismutase gene, the nucleotide sequence total length 745bp, such as SEQ ID
NO:Shown in 1.
There is provided a kind of preparation method of Cyanea capillata superoxide dismutase, this method for the second aspect of the present invention
Comprise the following steps：
(1) Cyanea capillata tentacle tissue cDNA library is built；
(2) sequence to above-mentioned Cyanea capillata tentacle tissue cDNA library recombinant clone is measured and analyzed, and obtains
The nucleotide sequence of encoding superoxide dismutase；
(3) expression plasmid of Cyanea capillata superoxide dismutase, recombination engineering are built；
(4) expression of Cyanea capillata superoxide dismutase；
(5) purifying of Cyanea capillata superoxide dismutase is recombinated.
Cyanea capillata tentacle tissue cDNA library in the step (1) is built by the following method：
1) Cyanea capillata tentacle total tissue RNA is extracted；
2) mRNA is separated, Cyanea capillata tentacle tissue cDNA is synthesized；
3) above-mentioned cDNA is inserted into pUC19 plasmid vectors, then converted into bacillus coli DH 5 alpha, be coated on LB culture mediums and put down
Plate carries out blue hickie screening, that is, is built into Cyanea capillata tentacle tissue cDNA library.
The expression plasmid of structure Cyanea capillata superoxide dismutase in the step (3), recombination engineering, specifically
Step is：
1) according to Cyanea capillata superoxide dismutase gene sequence and prokaryotic expression carrier pET24a digestion position
Point, a pair of PCR primers with specific cleavage site Nde I and Xho I of design are as follows：
Sense primer：5’-GGGAATTCCATATGGCTACATTGAAAGC-3’
Anti-sense primer：5’-CGCTCGAGCGCATTGGCTATT-3’
PCR reaction conditions are：95 DEG C of insulation 5min；95 DEG C of insulations 30sec, 55.5 DEG C of insulation 30sec, 68 DEG C of insulations
1min, 35 circulations；68 DEG C of insulation 5min；
2) PCR primer and pET24a prokaryotic expression plasmids after digestion is reclaimed；
3) coded sequence is connected on pET24a carriers using two restriction enzyme sites, builds recombinant expression plasmid, then
It is transformed into Escherichia coli Rosetta (DE3) pLysS and obtains recombination engineering.
The expression condition of Cyanea capillata superoxide dismutase in the step (4) is：12℃、0.5mMIPTG、
Induced 10 hours under 150rpm, recombinant protein can be expressed with soluble form under this inductive condition.
Purifying in the step (5) is to be produced using the step purifying of nickel ion affinity chromatograph post one, and elution requirement is difference
Account for the combination buffer of cumulative volume 50% and 50% elution buffer；Wherein, combination buffer is NaH2PO420mM；NaCl
500mM；Imidazoles 30mM；PH 7.4, elution buffer is NaH2PO420mM；NaCl 500mM；Imidazoles 500mM；pH 7.4.The system
Preparation Method is simple, with low cost.
The third aspect of the present invention is preparing antioxygen there is provided Cyanea capillata superoxide dismutase and its encoding gene
Application in chemical drug thing, antiradiation drug, shielding medicine for skin, food preservative, antiaging agent.
The restructuring Cyanea capillata superoxide dismutase that the present invention is obtained has significant antioxidation activity.In detection weight
Histone is in the experiment of ultra-oxygen anion free radical rejection ability, can be observed with restructuring Cyanea capillata copper zinc super oxygen
The inhibiting rate of superoxide anion is significantly increased in the rise of thing dismutase proteins concentration, reaction system, and superoxide dismutase reaches
Almost it is suppressed completely to superoxide anion during 500 μ g/mL levels.This experiment demonstrates restructuring Cyanea capillata copper zinc super oxygen
Scavenging activity of the thing mutase to ultra-oxygen anion free radical.
Therefore, the Cyanea capillata superoxide dismutase being related in the present invention is in exploitation anti-oxidation medicine, Kangshuaining mixture
There to be very big application value in terms of thing, antiradiation drug, shielding medicine for skin, food preservative.
Brief description of the drawings
Fig. 1 compares for the multiple sequence of Cyanea capillata superoxide dismutase and other species superoxide dismutases；
Wherein, black represents completely homologous region.
Fig. 2 is the PCR primer of amplification Cyanea capillata superoxide dismutase ORFs coded sequence in the present invention
Nucleic acid electrophoresis result；Wherein, M：2kb nucleic acid molecular weight Marker；1-12：PCR primer.
Fig. 3 is the SDS-PAGE electrophoresis results of restructuring Cyanea capillata superoxide dismutase induced expression in the present invention；
Wherein, M：Molecular weight of albumen Marker；1：The recombination bacillus coli not induced；2：Ultrasonic degradation liquid after being induced through 1mM IPTG；
3：Ultrasonic supernatant after being induced through 1mM IPTG；4：Ultrasound precipitation after being induced through 1mM IPTG；5：The recombination bacillus coli not induced；
6：Ultrasonic degradation liquid after being induced through 0.5mM IPTG；7：Ultrasonic supernatant after being induced through 0.5mM IPTG；8：Lured through 0.5mM IPTG
Lead rear ultrasound precipitation；Arrow indicates the position of recombinant protein.
Fig. 4 is the SDS-PAGE electrophoresis results that isolate and purify of restructuring Cyanea capillata superoxide dismutase in the present invention；
Wherein, 1：Cyanea capillata superoxide dismutase recombination bacillus coli before induction；2：Cyanea capillata super oxygen after induction
Compound mutase recombination bacillus coli ultrasonic degradation supernatant；3：Peak is penetrated during purification of recombinant proteins；4：Washed during purification of recombinant proteins
De- peak；Arrow indicates the position of recombinant protein.
Fig. 5 is detection purifying Cyanea capillata superoxide dismutase to ultra-oxygen anion free radical rejection ability (WST-1
Method) result；It can be observed with the rise of recombinant C uZnSOD protein concentrations, the inhibiting rate of superoxide anion in reaction system
Significantly increase, superoxide anion is almost suppressed completely when recombinant C uZnSOD reaches 500 μ g/mL levels.
Embodiment
With reference to embodiment and accompanying drawing, the present invention will be described in detail.But the following example should not be regarded as to the present invention
The limitation of scope.
Experimental method in following embodiments, is conventional method unless otherwise specified.
The Cyanea capillata (Cyanea capillata) that the present invention is selected is gathered from Zhejiang Province's nutrients, and through collection
Marine products institute of university of U.S. professor identifies (L.Xiao et al, Toxicon, 2009,53:146–152).
Embodiment 1：The structure of Cyanea capillata tentacle tissue cDNA library and analysis
1) extracting of Cyanea capillata tentacle total tissue RNA according to the Trizol kits of Invitrogen companies illustrate into
OK, chloroform removes isolating protein, obtains about 7 μ g total serum IgEs；
2) mRNA separation illustrates to carry out according to the Oligotex mRNA Spin-column Kit of QIANGEN companies,
CDNA synthesis is then carried out with reference to the SMART cDNA Library Construction Kit explanations of Clontech companies；
3) cDNA is inserted into pUC19 plasmid vectors (being purchased from Takara companies), then converted to bacillus coli DH 5 alpha (purchased from north
Jing Bomaide companies) in, it is coated on 15CM culture dishes and carries out blue hickie screening, that is, is built into Cyanea capillata tentacle tissue cDNA
Library.
The library has bacterium colony 1923, wherein locus coeruleus 35, recombination fraction 98.18%, storage capacity 1.92*106, insertion length
These sequences are carried out unigene merger using 100bp, 90% principle, obtained by degree >=400bp ordered sequence 1035
Unigene numbers are 528.Total length analysis is carried out to sequence simultaneously, there are 12 sequences to have associated homologous information in 20 sequences,
As a result it is wherein 12 total lengths, complete sex rate：12/12 × 100%=100%, shows that the cDNA library has preferable matter
Amount.Random sequencing is carried out to the cDNA library, gained sequence carries out BLASTx analyses (http after removing carrier://
blast.ncbi.nlm.nig.gov)。
4) Cyanea capillata superoxide dismutase gene numbers the clone for being 13F9 in above-mentioned cDNA library.Sequence
Total length 745bp, includes 465bp ORFs, protein of the coding containing 154 amino acid residues, molecular weight
16.0kDa, isoelectric point 6.2.Blastx search results show the superoxide dismutase very high homology of the albumen and multiple species
(such as Fig. 1), is the recruit of jellyfish source superoxide dismutase family.Its further bioinformatic analysis is shown,
The albumen does not contain signal peptide, is non-secreted protein, is positioned at cytoplasm.
Embodiment 2：Structure and the engineering bacteria restructuring of Cyanea capillata superoxide dismutase recombinant expression plasmid
1) (it is purchased from according to Cyanea capillata superoxide dismutase gene sequence and prokaryotic expression carrier pET24a
Novagen companies) restriction enzyme site, design and synthesize pair of primers, wherein sense primer includes restriction enzyme site Nde I
(CATATG), anti-sense primer includes restriction enzyme site Xho I (CTCGAG), and the sequence of two primers is specific as follows：
Sense primer：5’-GGGAATTCCATATGGCTACATTGAAAGC-3’(SEQ ID NO:3)
Anti-sense primer：5’-CGCTCGAGCGCATTGGCTATT-3’(SEQ ID NO:4)
After grads PCR is tested, it is optimum annealing temperature to select 55.5 DEG C, enters performing PCR to target gene and largely expands,
PCR reaction conditions are：95 DEG C of insulation 5min；95 DEG C of insulations 30sec, 55.5 DEG C of insulations 30sec, 68 DEG C of insulation 1min, 35 are followed
Ring；68 DEG C of insulation 5min.5 ' the ends PCR primer comprising Nde I and the restriction enzyme sites of Xho I, nov nucleic acid respectively are obtained after PCR reactions
Swimming shows PCR primer band at correct position (such as Fig. 2), and about 481bp reclaims PCR primer；
2) PCR after being reclaimed according to the description of product with Nde I and Xho I endonuclease the difference digestion of NEB companies is produced
Thing and pET24a prokaryotic expression plasmids；
3) reclaim the T4DNA ligases explanation after digestion products further according to NEB companies and be attached reaction, by coupled reaction
Product is converted to Escherichia coli Rosetta (DE3) pLysS (being purchased from Beijing Bo Maide companies) and is coated on containing kanamycins (100
μ g/ml) and the culture dish of chloramphenicol (34 μ g/ml) on cultivate 15 hours.Picking single bacterium is dropped down onto has kanamycins (100 containing 5ml
μ g/ml) and chloramphenicol (34 μ g/ml) LB culture mediums shake bacterium cultivate 12 hours, through bacterium solution PCR, plasmid enzyme restriction verify etc. method
After identification is recombinated successfully, two-way sequencing is carried out to recombinant plasmid by sequencing primer of T7Terminator, the gene of clone is verified
For purpose gene, illustrate that Cyanea capillata superoxide dismutase recombinant expression plasmid is correctly built.
Embodiment 3：The expression of Cyanea capillata superoxide dismutase
Correct recombination bacillus coli Rosetta (DE3) pLysS bacterium solutions will be sequenced and add (100 μ g/ml) containing kanamycins
In the LB liquid medium of chloramphenicol (34 μ g/ml), 37 DEG C, 250rpm shaking table cultures to OD600Start to add during for 0.6-0.8
Enter derivant IPTG to be induced.
The optimum condition of the expression for determining recombinant protein is：12 DEG C, induce 10 hours under 0.5mM IPTG, 150rpm.Induction
After thalline is collected by centrifugation, can be expressed through the visible recombinant protein under this inductive condition of SDS-PAGE electrophoresis with soluble form, point
Son amount is consistent with predicted value (adding about 17.2kDa after His labels), as shown in Figure 3.
Embodiment 4：Recombinate the purifying of Cyanea capillata superoxide dismutase
Thalline is collected into bacterium solution centrifugation (10000 × g*10min) after induction, then using combination buffer
(NaH2PO420mM；NaCl 500mM；Imidazoles 30mM；PH=7.4 thalline) is fully resuspended, carries out ultrasound and splits bacterium, ultrasonic degradation liquid
Supernatant is taken to be purified after centrifugation (10000 × g*10min).Final HP layers of the HisTrap according to GE Healthcare companies
Analyse post andThe operating instruction for isolating and purifying system carries out nickel ion affinity chromatograph, that is, obtains purer hair
Shape rosy clouds jellyfish superoxide dismutase.
SDS-PAGE electrophoresis (such as Fig. 4) visible purpose band is consistent with predicted position, and purity is more than 90%, and what is used washes
De- condition is：50% combination buffer and 50% elution buffer (NaH2PO420mM；NaCl 500mM；Imidazoles 500mM；
pH 7.4)。
Embodiment 5：Recombinate the detection that Cyanea capillata superoxide dismutase removes superoxide anion ability
Obtained by the nickel ion affinity chromatograph method of embodiment 4 after more single destination protein, use colleague's chemistry
Superoxide dismutase (SOD) detection kit (WST-1 methods) of research institute (Dojindo) exploitation is detected, is said by producer
The bright quasi- flow operations of book label.
The subsidiary high water soluble tetrazolium salts WST-1 of the kit (2- (4- iodophenyls) -3- (4- nitrobenzophenones) -5- (2,
4- disulfonic acid phenyl) -2 hydrogen-tetrazolium salts, disodium salt) can be with superoxide anion (O2 -) a kind of water miscible dyestuff of reaction generation,
But superoxide anion is preferentially occurred by the SOD reactions suppressed, therefore can determine recombinant protein rCcCuZnSOD by colorimetric method
To the rejection ability of ultra-oxygen anion free radical.
Experimental result surpasses in reaction system as shown in figure 5, can be observed with the rise of recombinant C uZnSOD protein concentrations
The inhibiting rate of oxygen anion is significantly increased, and superoxide anion is almost pressed down completely when recombinant C uZnSOD reaches 500 μ g/mL levels
System.
Illustrating the restructuring Cyanea capillata superoxide dismutase of the present invention has significant antioxidation activity, available for opening
Send out anti-oxidation medicine, antiaging agent, antiradiation drug, shielding medicine for skin, food preservative etc..
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, various changes and modifications of the present invention are possible without departing from the spirit and scope of the present invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent is defined.
Claims (7)
1. a kind of Cyanea capillata superoxide dismutase, it is characterised in that described Cyanea capillata superoxide dismutase
Amino acid sequence such as SEQ ID NO:Shown in 2.
2. a kind of encoding gene of Cyanea capillata superoxide dismutase as claimed in claim 1, it is characterised in that the volume
The nucleotide sequence such as SEQ ID NO of code gene:Shown in 1.
3. a kind of preparation method of Cyanea capillata superoxide dismutase as claimed in claim 1, it is characterised in that the party
Method comprises the following steps：
(A) Cyanea capillata tentacle tissue cDNA library is built；
(B) sequence to above-mentioned Cyanea capillata tentacle tissue cDNA library recombinant clone is measured and analyzed, and obtains right
It is required that the nucleotide sequence of the coding Cyanea capillata superoxide dismutase described in 2；Touched using PCR method from Cyanea capillata
Hand tissue CDNA is expanded in library to the sequence；
(C) expression plasmid of Cyanea capillata superoxide dismutase, recombination engineering are built；
(D) expression of Cyanea capillata superoxide dismutase；
(E) purifying of Cyanea capillata superoxide dismutase is recombinated.
4. the preparation method of Cyanea capillata superoxide dismutase according to claim 3, it is characterised in that：The step
Suddenly the expression plasmid of the structure Cyanea capillata superoxide dismutase in (C), recombination engineering concretely comprises the following steps：
A) PCR primer is designed and synthesized as follows：
Sense primer such as SEQ ID NO:Shown in 3,
Anti-sense primer such as SEQ ID NO:Shown in 4,
PCR reaction conditions are：95 DEG C of insulation 5min；95 DEG C of insulations 30sec, 55.5 DEG C of insulation 30sec, 68 DEG C are incubated 1min, 35
Individual circulation；68 DEG C of insulation 5min；
B) PCR primer and pET24a prokaryotic expression plasmids after digestion is reclaimed；
C) coded sequence is connected on pET24a carriers using two restriction enzyme sites, builds recombinant expression plasmid, then convert
Recombination engineering is obtained to Escherichia coli Rosetta.
5. the preparation method of Cyanea capillata superoxide dismutase according to claim 3, it is characterised in that：The step
Suddenly the expression condition of the Cyanea capillata superoxide dismutase in (D) is：12 DEG C, lure in 0.5mM IPTG, 150rpm environment
Lead 10 hours.
6. a kind of Cyanea capillata superoxide dismutase as claimed in claim 1 is preparing anti-oxidation medicine, antiradiation drug
Application in thing, shielding medicine for skin, food preservative or antiaging agent.
7. a kind of encoding gene of Cyanea capillata superoxide dismutase as claimed in claim 2 is preparing antioxidant drug
Application in thing, antiradiation drug, shielding medicine for skin, food preservative or antiaging agent.
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| CN107828749B (en) * | 2017-12-15 | 2020-03-31 | 中国科学院深海科学与工程研究所 | Low-temperature-resistant superoxide dismutase (MnSOD) from deep sea cucumber and preparation method thereof |
| CN107955806B (en) * | 2017-12-15 | 2020-03-31 | 中国科学院深海科学与工程研究所 | Preparation method and application of superoxide dismutase Cu, Zn SOD (superoxide dismutase) from deep-sea cucumber |
| CN112480224A (en) * | 2020-11-19 | 2021-03-12 | 中国人民解放军海军特色医学中心 | Marine-derived antioxidant protein of fusion expression cell-penetrating peptide and preparation method and application thereof |
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