CN104478987A - Special extracting solution for oil palm leaf total proteins and extracting method thereof - Google Patents
Special extracting solution for oil palm leaf total proteins and extracting method thereof Download PDFInfo
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 90
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 90
- 235000001950 Elaeis guineensis Nutrition 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 49
- 240000003133 Elaeis guineensis Species 0.000 title description 2
- 241000512897 Elaeis Species 0.000 claims abstract description 83
- 239000006228 supernatant Substances 0.000 claims abstract description 24
- 239000000284 extract Substances 0.000 claims description 55
- 238000000605 extraction Methods 0.000 claims description 22
- 230000009514 concussion Effects 0.000 claims description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 229910021538 borax Inorganic materials 0.000 claims description 9
- 239000004328 sodium tetraborate Substances 0.000 claims description 9
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 9
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 9
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 230000001186 cumulative effect Effects 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 235000019154 vitamin C Nutrition 0.000 claims description 7
- 239000011718 vitamin C Substances 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000008139 complexing agent Substances 0.000 claims description 4
- 230000002829 reductive effect Effects 0.000 claims description 4
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- IMONTRJLAWHYGT-ZCPXKWAGSA-N Norethindrone Acetate Chemical group C1CC2=CC(=O)CC[C@@H]2[C@@H]2[C@@H]1[C@@H]1CC[C@](C#C)(OC(=O)C)[C@@]1(C)CC2 IMONTRJLAWHYGT-ZCPXKWAGSA-N 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 7
- 238000000751 protein extraction Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract 1
- 239000012460 protein solution Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 71
- 241000196324 Embryophyta Species 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Substances CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 2
- 101710138460 Leaf protein Proteins 0.000 description 2
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 description 2
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 description 2
- 239000001253 polyvinylpolypyrrolidone Substances 0.000 description 2
- 244000080767 Areca catechu Species 0.000 description 1
- 235000006226 Areca catechu Nutrition 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- Peptides Or Proteins (AREA)
Abstract
The invention relates to the field of proteomics research and discloses a special extracting solution for oil palm leaf total proteins and an extracting method thereof. The extracting method comprises the following steps: adding the oil palm special extracting solution into oil palm leaves which are ground into powder, performing total protein extraction of the oil palm leaves, wherein the extracted liquid supernatant amount and the concentration of the extracted proteins are obviously increased according to quantitative measurement. Over 3mg of total proteins can be obtained from every 2g of oil palm leaves; and the extracted protein solution is clear in color, not or slightly thick and greatly improved in purity, so that the follow-up study is greatly convenient.
Description
Technical field
The present invention relates to proteomics research field, refer to a kind of special extract and extracting method of oil palm leaves total protein matter especially.
Background technology
In the research of proteomics, in plant, the extraction of protein is the basis of all research, also directly affects the result of subsequent experimental.At present, method conventional in the extraction of plant protein has trichoroacetic acid(TCA)-acetone precipitation (TCA) method, E-TCA method and phenol (Phe) to take out method.Although above method can remove the impurity of the interferencing protein analytical resultss such as polysaccharide, ester class and phenols plant tissue from different aspect, unsatisfactory at the extraction effect of tropical crops protein.Chinese invention patent CN1908007A discloses extracting method and the special extract thereof of a kind of plant total protein, proposes the extracting method of a kind of plant total protein, is applicable to majority of plant, the especially extraction of gross protein in halophytes.Due to three kinds of important chemical composition sodium tetraborate (Borax), polyvinylpolypyrrolidone (PVPP) and phenol (Phenol) required in leaching process, so called after BPP method.Use BPP method to extract tropical crops, as coconut, betel nut, banana, rubber, wood work waits the extraction of plant leaf gross protein, also achieves good effect.
Oil palm (Elaeis guineensis) belongs to perennial monocotyledons, rich in oil in the pulp of oil palm, kernel, in various oil crops, has the title of " world oil king ".Utilize BPP method to extract its leaves total protein, in leaching process, supernatant liquor comparatively thickness, very difficult with pipettor Aspirate supernatant, and cause oil palm blade extraction efficiency to reduce, total protein purity is not high, has had a strong impact on the carrying out of subsequent experimental.This phenomenon summer temperature higher be particularly serious, often cause total protein extraction failure.
Summary of the invention
The present invention proposes a kind of special extract and extracting method of oil palm leaves total protein matter, effectively can remove the thick substances in supernatant liquor, improve total protein extraction productive rate and purity.
Technical scheme of the present invention is achieved in that
A kind of special extract of oil palm leaves total protein matter, with the cumulative volume of described oil palm leaves total protein matter special extract note, comprise the component of following mass concentration: complexing agent 35-40g/L, sodium tetraborate 15-20g/L, reductive agent 20-40g/L, sucrose 200-400g/L; The special extract pH value of described oil palm gross protein is 4-7.
Further, described complexing agent is ENTA or EDTA-2Na.
Further, described reductive agent is the mixture of vitamins C and beta-mercaptoethanol.
Further, ascorbic mass concentration is 5-10g/L, and the mass concentration of beta-mercaptoethanol is 15-28g/L.
Further, the special extract pH value of described oil palm leaves total protein matter is 4-5.
Further, described pH value regulates reagent used to be hydrochloric acid.
A kind of extracting method of oil palm leaves total protein matter, comprise the following steps: oil palm blade is pulverized last, add described oil palm leaves total protein matter special extract, room temperature whirlpool concussion 4-6min, remove supernatant liquor after centrifugal, then carry out the extraction of gross protein according to BPP method.
Further, with leaf quality note, the amount that described oil palm leaves total protein matter special extract adds is 4-6 (V/W) times amount.
Further, in described centrifugal process, temperature is 3-5 DEG C, centrifugal force 10000-17000RCF, and centrifugation time is 8-12min.
Beneficial effect of the present invention:
Oil palm special extract of the present invention, join after oil palm blade grinds in powder, effectively can remove the dope in supernatant liquor, greatly reduce the difficulty of experimental implementation, and leaching process is not by the impact of temperature, summer or winter all can carry out experimental implementation, easy and simple to handle, for oil palm leaf protein Quality Research provides the foundation.Extract the protein soln color clarification obtained, not or only have minute quantity thick substances, purity does not improve greatly.Carry out detection by quantitative through Bradford method to the protein soln extracted, the total protein of the oil palm blade adopting BPP method to extract, gained extracting solution of protein concentration is generally 5-7 μ g μ L
-1, and adopting extracting method of the present invention, gained total protein concentration generally can reach 8 μ g μ L
-1above, most concentration is at 10 μ g μ L
-1left and right.Meanwhile, after testing, carry out the extraction of oil palm leaves total protein by adding oil palm special extract of the present invention, gross protein extraction yield improves greatly.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
A special extract for oil palm leaves total protein matter, with the cumulative volume of described oil palm leaves total protein matter special extract note, comprises the component of following mass concentration: EDTA 35g/L, sodium tetraborate 15g/L, vitamins C 5g/L, beta-mercaptoethanol 15g/L, sucrose 200g/L; Add concentrated hydrochloric acid and regulate pH value to 4.
An extracting method for the special extract that oil palm leaves total protein matter is extracted, comprises the following steps: oil palm blade is pulverized last, adds oil palm leaves total protein matter special extract of the present invention; With leaf quality note, the amount that described oil palm leaves total protein matter special extract adds is 4 (V/W) times amount, and room temperature whirlpool concussion 4min, removes supernatant liquor after centrifugal, then carry out the extraction of leaves total protein matter according to BPP method.Concrete operation step is as follows:
(1) get oil palm blade 2g, liquid nitrogen grinding powdered, proceed to 50mL centrifuge tube;
(2) add 8mL oil palm leaves total protein matter special extract, room temperature whirlpool concussion 4min, removes supernatant after centrifugal 8min, repeats this step 1 time under temperature 3 DEG C, centrifugal force 10000RCF condition;
(3) 10mL precooling extracting solution, 1mL 10%SDS is added, room temperature whirlpool concussion 5min;
(4) equal-volume Tris balance phenol (pH7.8) is added, room temperature whirlpool concussion 10min;
(5) 4 DEG C, 17000RCF, centrifugal 15min, upper-layer phenol goes to 10mL centrifuge tube mutually;
(6) equal-volume precooling extracting solution is added, room temperature whirlpool concussion 10min;
(7) 4 DEG C, 17000RCF, centrifugal 15min, upper-layer phenol goes to new pipe mutually;
(8) add the pre-cold methanol of 5 times of volumes containing supersaturated ammonium sulfate, mixing, puts-20 DEG C of more than precipitating proteins 6h or spends the night.
(9) supernatant is removed, resuspended 2 times of protein precipitation precooling methanol solution after centrifugal (4 DEG C, 17000RCF, 10min);
(10) remove supernatant after centrifugal (4 DEG C, 17000RCF, 10min), with precooling acetone soln resuspended for 2 times;
(11) centrifugal (4 DEG C, 17000RCF, 10min), precipitate air-dry after add appropriate lysate, more than room-temperature dissolution 2h.
(12) centrifugal (4 DEG C, 17000RCF, 30min), draws supernatant for subsequent use to 1.5mL centrifuge tube.
Embodiment 2
The special extract that oil palm leaves total protein matter is extracted, with the cumulative volume of described oil palm leaves total protein matter special extract note, comprises the component of following mass concentration: EDTA-2Na 37g/L, sodium tetraborate 18g/L, vitamins C 8g/L, beta-mercaptoethanol 22g/L, sucrose 300g/L; Add acetic acid and regulate pH value to 5.5.
An extracting method for the special extract that oil palm leaves total protein matter is extracted, comprises the following steps: oil palm blade is pulverized last, adds oil palm leaves total protein matter special extract of the present invention; With leaf quality note, the amount that described oil palm leaves total protein matter special extract adds is 5 (V/W) times amount, and room temperature whirlpool concussion 5min, removes supernatant liquor after centrifugal, then carry out the extraction of leaves total protein matter according to BPP method.
Its concrete operation step difference from Example 1 is, the add-on of step (2) oil palm leaves total protein matter special extract is 10mL, room temperature whirlpool concussion 5min; Under temperature 4 DEG C, centrifugal force 13000RCF condition, remove supernatant liquor after centrifugal 10min, all the other operations are identical with embodiment 1.
Embodiment 3
The special extract that oil palm leaves total protein matter is extracted, with the cumulative volume of described oil palm leaves total protein matter special extract note, comprises the component of following mass concentration: EDTA 40g/L, sodium tetraborate 20g/L, vitamins C 10g/L, beta-mercaptoethanol 28g/L, sucrose 400g/L; Add hydrochloric acid and regulate pH value to 7.
An extracting method for oil palm leaves total protein matter, comprises the following steps: oil palm blade is pulverized last, adds oil palm leaves total protein matter special extract of the present invention; With leaf quality note, the amount that described oil palm leaves total protein matter special extract adds is 8 (V/W) times amount, and room temperature whirlpool concussion 6min, removes supernatant liquor after centrifugal, then carry out the extraction of leaves total protein matter according to BPP method.
Its concrete operation step difference from Example 1 is, step (2) adds 16mL oil palm leaves total protein matter special extract, room temperature whirlpool concussion 5min, removes supernatant liquor after centrifugal 12min, repeats this step 1 time under temperature 5 DEG C, centrifugal force 17000RCF condition; All the other operations are identical with embodiment 1.
Embodiment 4
The special extract that oil palm leaves total protein matter is extracted, with the cumulative volume of described oil palm leaves total protein matter special extract note, comprises the component of following mass concentration: EDTA 35g/L, sodium tetraborate 18g/L, vitamins C 5g/L, beta-mercaptoethanol 28g/L, sucrose 300g/L; Add hydrochloric acid and regulate pH value to 5.
An extracting method for oil palm leaves total protein matter, comprises the following steps: oil palm blade is pulverized last, adds oil palm leaves total protein matter special extract of the present invention; With leaf quality note, the amount that described oil palm leaves total protein matter special extract adds is 6 (V/W) times amount, and room temperature whirlpool concussion 6min, removes supernatant liquor after centrifugal, then carry out the extraction of leaves total protein matter according to BPP method.
Its concrete operation step difference from Example 1 is, step (2) adds 12mL oil palm leaves total protein matter special extract, room temperature whirlpool concussion 6min, removes supernatant liquor after centrifugal 12min, repeats this step 1 time under temperature 5 DEG C, centrifugal force 10000RCF condition; All the other operations are identical with embodiment 1.
Embodiment 5
The special extract that oil palm leaves total protein matter is extracted, with the cumulative volume of described oil palm leaves total protein matter special extract note, comprises the component of following mass concentration: EDTA 37g/L, sodium tetraborate 19g/L, vitamins C 10g/L, beta-mercaptoethanol 22g/L, sucrose 200g/L; Add hydrochloric acid and regulate pH value to 4.5.
An extracting method for oil palm leaves total protein matter, comprises the following steps: oil palm blade is pulverized last, adds oil palm leaves total protein matter special extract of the present invention; With leaf quality note, the amount that described oil palm leaves total protein matter special extract adds is 5 (V/W) times amount, and room temperature whirlpool concussion 5min, removes supernatant liquor after centrifugal, then carry out the extraction of leaves total protein matter according to BPP method.
Its concrete operation step difference from Example 1 is, step (2) adds 10mL oil palm leaves total protein matter special extract, and room temperature whirlpool concussion 5min, removes supernatant liquor after centrifugal 10min under temperature 3 DEG C, centrifugal force 17000RCF condition; All the other operations are identical with embodiment 1.
In above embodiment, if thick substances is more in supernatant liquor, step (2) can be repeated 1-2 time.The special extract of oil palm leaves total protein of the present invention and extracting method effect expedition
Get 2g oil palm blade respectively, respectively according to extracting method and the conventional BPP method extraction leaves total protein matter of the present invention's three embodiments, each process repeats 10 operations.Obtaining extracting solution of protein to extraction adopts Bradford method to carry out quantitative assay, and calculate its mean value, result is as shown in table 1.
Table 1 extracting method extraction effect of the present invention compares
As shown in Table 1, the method that three embodiments of the present invention provide, the supernatant liquid measure that proteins extraction obtains, protein concn are all significantly increased.The ultimate capacity of 2g oil palm leaves total protein matter is all at more than 3mg; And adopting BPP method, the protein ultimate capacity of gained is at about 2mg.
Adopt BPP method, the supernatant fluid color extracting gained is faint yellow, and obvious thickness, foreign matter content is high, brings very large impact to follow-up research.And the treatment process described in the present invention's three embodiments, do not extract the supernatant liquor that obtains not or only have the thick substances of minute quantity, color is clarified, and foreign matter content reduces greatly, facilitates the carrying out of subsequent experimental research.In addition, extracting method provided by the present invention, extract operation not by the restriction of envrionment temperature, temperature high (summer), temperature low (winter) all can carry out the extraction of oil palm leaves total protein matter, greatly improve the efficiency that oil palm leaves total protein extracts, the correlative study also for carrying out oil palm leaf protein under Various Seasonal and weather condition creates condition.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. a special extract for oil palm leaves total protein matter, is characterized in that, remembers with the cumulative volume of described oil palm leaves total protein matter special extract, comprise the component of following mass concentration: complexing agent 35-40g/L, sodium tetraborate 15-20g/L, reductive agent 20-40g/L, sucrose 200-400g/L; The special extract pH value of described oil palm gross protein is 4-7.
2. the special extract of a kind of oil palm leaves total protein matter extraction according to claim 1, is characterized in that: described complexing agent is ENTA or EDTA-2Na.
3. the special extract of a kind of oil palm leaves total protein matter extraction according to claim 1, is characterized in that: described reductive agent is the mixture of vitamins C and beta-mercaptoethanol.
4. the special extract of a kind of oil palm leaves total protein matter extraction according to claim 3, it is characterized in that: ascorbic mass concentration is 5-10g/L, the mass concentration of beta-mercaptoethanol is 15-28g/L.
5. the special extract of a kind of oil palm leaves total protein matter according to claim 1, is characterized in that: the special extract pH value of described oil palm gross protein is 4-5.
6. the special extract of a kind of oil palm leaves total protein matter according to claim 1, is characterized in that: the reagent regulating pH value used is hydrochloric acid.
7. the extracting method of an oil palm leaves total protein matter, comprise the following steps: oil palm blade is pulverized last, add oil palm leaves total protein matter special extract described in the arbitrary claim of claim 1-6, room temperature whirlpool concussion 4-6min, remove supernatant liquor after centrifugal, then carry out the extraction of leaves total protein matter according to BPP method.
8. the extracting method of a kind of oil palm gross protein according to claim 7, is characterized in that: the volume added of described oil palm leaves total protein matter special extract is oil palm leaf quality 4-6 times (V/W).
9. a kind of oil palm leaves total protein matter extracting method according to claim 7, it is characterized in that: in described centrifugal process, temperature is 3-5 DEG C, centrifugal force 10000-17000RCF, and centrifugation time is 8-12min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859831A (en) * | 2016-04-18 | 2016-08-17 | 中国农业科学院果树研究所 | Apple protein extraction method and related protein extracting solution |
| CN106939032A (en) * | 2017-03-24 | 2017-07-11 | 艾比玛特医药科技（上海）有限公司 | A kind of extract solution and extracting method for rapidly and efficiently extracting plant total protein |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1908007A (en) * | 2006-08-07 | 2007-02-07 | 中国科学院植物研究所 | Extraction method of plant total protein and special extract for the same |
| WO2009064404A2 (en) * | 2007-11-15 | 2009-05-22 | Univation Technologies, Llc | Polymeriazation catalysts, methods of making; methods of using, and polyolefinproducts made therefrom |
| CN102532258A (en) * | 2012-03-15 | 2012-07-04 | 中国热带农业科学院热带生物技术研究所 | Method for extracting protein from component with low protein content |
-
2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1908007A (en) * | 2006-08-07 | 2007-02-07 | 中国科学院植物研究所 | Extraction method of plant total protein and special extract for the same |
| WO2009064404A2 (en) * | 2007-11-15 | 2009-05-22 | Univation Technologies, Llc | Polymeriazation catalysts, methods of making; methods of using, and polyolefinproducts made therefrom |
| CN102532258A (en) * | 2012-03-15 | 2012-07-04 | 中国热带农业科学院热带生物技术研究所 | Method for extracting protein from component with low protein content |
Non-Patent Citations (1)
| Title |
|---|
| 王海燕等: "巴西橡胶树幼叶和成熟叶比较蛋白质组学初步研究", 《中国农学通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859831A (en) * | 2016-04-18 | 2016-08-17 | 中国农业科学院果树研究所 | Apple protein extraction method and related protein extracting solution |
| CN106939032A (en) * | 2017-03-24 | 2017-07-11 | 艾比玛特医药科技（上海）有限公司 | A kind of extract solution and extracting method for rapidly and efficiently extracting plant total protein |
| CN106939032B (en) * | 2017-03-24 | 2021-06-22 | 艾比玛特医药科技（上海）有限公司 | Extracting solution for rapidly and efficiently extracting total plant protein and extracting method |
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| CN104478987B (en) | 2018-06-19 |
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