CN104458857B - Preparation method of kaempferol molecularly imprinted membrane based electrochemical sensor - Google Patents
Preparation method of kaempferol molecularly imprinted membrane based electrochemical sensor Download PDFInfo
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- CN104458857B CN104458857B CN201410761732.2A CN201410761732A CN104458857B CN 104458857 B CN104458857 B CN 104458857B CN 201410761732 A CN201410761732 A CN 201410761732A CN 104458857 B CN104458857 B CN 104458857B
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Abstract
The invention discloses a preparation method of a kaempferol molecularly imprinted membrane based electrochemical sensor. The preparation method of the kaempferol molecularly imprinted membrane based electrochemical sensor comprises the following steps of: sanding and polishing a glassy carbon electrode with 0.3mu m Al2O3 suspension and 0.05mu m Al2O3 suspension in sequence on deerskin, washing with water, putting into 1:1 nitric acid, ethanol and secondary water in sequence, washing ultrasonically for 5 minutes, and finally washing with secondary water for later use; putting a three-electrode system into a prepared Na2HPO4-NaH2PO4 buffer solution, carrying out cyclic voltammetry scan on the three-electrode system for 20 rounds at the scan speed of 50mV/s in the potential range of 0 to 0.8V, putting the three electrodes into sodium hydroxide solution for 30 minutes to remove kaempferol (a template molecule) out of a molecularly imprinted polymer to obtain a kaempferol molecularly imprinted electrode, wherein the three-electrode system comprises the glassy carbon electrode serving as a working electrode, the platinum sheet electrode serving as a counter electrode, the saturated calomel electrode serving as a reference electrode, the concentrations of kaempferol and o-phenylenediamine in the Na2HPO4-NaH2PO4 buffer solution are 0.2mmol/l and 4mmol/l respectively, and the pH value of the Na2HPO4-NaH2PO4 buffer solution is controlled at around 6.0.
Description
Technical field
The present invention relates to a kind of preparation method of blotting membrane electrochemical sensor.
Background technology
Molecularly imprinted polymer（MIPs）It is a kind of high polymer material for certain molecule with specific recognition capability,
Due to it have structure effect precordainment, specific selectivity, be easy to implement many advantages, such as, chromatographic isolation, Solid-Phase Extraction, food,
The fields such as membrance separation, bionical sensing, capillary electric chromatogram and clinical medicine are all widely used.
Kaempferol（Kaempferol）Also known as kaempferol, Kaempferol etc..Root from zingiberaceous plant Rhizoma Kaempferiae
Stem, the rhizome of Berberidaceae plant Rhizoma et Radix Diphylleiae, sandalwood plants Herba Thesii, the fruit of the drying and ripening of leguminous plant Sophora japonica L..Antibacterial,
All there is inhibitory action to Staphylococcus aureus and bacillus pyocyaneus, Bacillus typhi, dysentery bacterium.Cough-relieving, treats bronchitis.Suppression
Enzyme, suppresses eye aldose reductase, is conducive to the treatment of diabetic cataract.There is mutagenic agent activity, when concentration is 1 × 10-
During 4mol/L, lymphopoiesis can be suppressed.Be mainly used in anticancer, suppression fertility, epilepsy, antiinflammatory, antioxidant, spasmolytic,
Antiulcer, function of gallbladder promoting diuretic, cough-relieving.The structural formula of kaempferol is as follows：
.
Content of the invention
The technical problem to be solved in the present invention is to overcome existing defect, there is provided a kind of kaempferol molecular engram film electrification
Learn the preparation method of sensor.
The purpose of the present invention to implement by the following technical programs：
A kind of preparation method of kaempferol molecular engram film electrochemical sensor, comprises the steps：
1）The pretreatment of electrode
Glass-carbon electrode is successively with 0.3,0.05 μm of Al2O3Suspension, after sanding and polishing on Corium Cervi, is cleaned up with water
Afterwards, electrode is sequentially placed into 1:In 1 nitric acid, dehydrated alcohol and secondary water, it is cleaned by ultrasonic 5min, is finally rinsed well with secondary water
Stand-by；
2）The preparation of electrode
By glass-carbon electrode be working electrode, platinum plate electrode be to electrode, saturated calomel electrode be reference electrode three electrodes
System puts into the Na having configured2HPO4-NaH2PO4In the middle of buffer solution, in the middle of this solution, contain kaempferol, o-phenylenediamine, concentration
It is respectively 0.2mmol/l and 4mmol/l, pH value controls in 6.0 about, in 0～0.8V potential region, cyclic voltammetry scan
20 circles, sweep speed is 50mV/s, is then positioned over 30 minutes in sodium hydroxide solution by electrode, to remove in imprinted polymer
Template molecule-kaempferol, that is, obtain kaempferol molecular engram electrode.
Beneficial effects of the present invention：
First with o-phenylenediamine as function monomer, kaempferol is template molecule to the present invention, by electrochemical polymerization in glass carbon
Electrode surface is prepared for kaempferol molecular engram film, using cyclic voltammetry, is prepared for kaempferol molecular engram film sensors, this
The trace electrode film of method preparation has higher selectivity and sensitivity to kaempferol, and the range of linearity is 1.0 × 10-6～2.0
×10-5Mol/l, detection are limited to 3.3 × 10-7mol/l.
Specific embodiment
Hereinafter the preferred embodiments of the present invention are illustrated it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
The preparation method of kaempferol molecular engram film electrochemical sensor：
Glass-carbon electrode successively with 0.3,0.05 μm of Al2O3 suspension after sanding and polishing on Corium Cervi, cleaned up with water
Afterwards, electrode is sequentially placed into 1:In 1 nitric acid, dehydrated alcohol and secondary water, it is cleaned by ultrasonic 5min, is finally rinsed well with secondary water
Stand-by.The good glass-carbon electrode of pretreatment is placed in 0.5 mol/L sulfuric acid solution, in -0.6～+0.8V potential region, with 50
Scanning speed scanning 20 circle of mV/s, until cyclic voltammetry curve is stable.Will be same to glass-carbon electrode, saturated calomel electrode, platinum electrode
When, put in the middle of the Na2HPO4-NaH2PO4 buffer solution having configured, and contains kaempferol, o-phenylenediamine, concentration in the middle of this solution
It is respectively 0.2mmol/l and 4mmol/l, pH value controls 6.0 about.In 0～0.8V potential region, cyclic voltammetry scan
20 circles, sweep speed is 50mV/s.Then electrode is positioned over 30 minutes in sodium hydroxide solution, to remove in imprinted polymer
Template molecule-kaempferol, that is, obtain kaempferol molecular engram electrode.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to aforementioned reality
Apply example the present invention has been described in detail, for a person skilled in the art, it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or carries out equivalent to wherein some technical characteristics.All essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (1)
1. a kind of preparation method of kaempferol molecular engram film electrochemical sensor it is characterised in that：Comprise the steps：
1）The pretreatment of electrode
Glass-carbon electrode is successively with 0.3,0.05 μm of Al2O3Suspension, after sanding and polishing on Corium Cervi, after being cleaned up with water, is incited somebody to action
Electrode is sequentially placed into 1:In 1 nitric acid, dehydrated alcohol and secondary water, it is cleaned by ultrasonic 5min, is finally rinsed well with secondary water stand-by；
2）The preparation of electrode
By glass-carbon electrode be working electrode, platinum plate electrode be to electrode, saturated calomel electrode be reference electrode three-electrode system
Put into the Na having configured2HPO4-NaH2PO4In the middle of buffer solution, in the middle of this solution, contain kaempferol, o-phenylenediamine, concentration is respectively
For 0.2mmol/l and 4mmol/l, pH value controls 6.0 about, and in 0～0.8V potential region, cyclic voltammetry scan 20 encloses,
Sweep speed is 50mV/s, is then positioned over 30 minutes in sodium hydroxide solution by electrode, to remove the mould in imprinted polymer
Plate molecule-kaempferol, that is, obtain kaempferol molecular engram electrode.
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| CN104458857B true CN104458857B (en) | 2017-02-22 |
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| CN105973958B (en) * | 2016-05-05 | 2018-03-27 | 济南大学 | A kind of preparation method of fluorouracil molecular imprinting electrochemical sensor |
| IT201700026417A1 (en) | 2017-03-09 | 2017-06-09 | Univ Ca Foscari Di Venezia | NEW MOLECULAR MOLD ELECTROCHEMICAL SENSORS FOR PERFLUOROOTTANSOLPHONATE AND ITS DERIVATIVES |
| CN108107095A (en) * | 2017-12-13 | 2018-06-01 | 河南师范大学 | The preparation method and application of salicylic acid molecular imprinting electrochemical sensor based on nanometer modified by silver |
| CN107991361A (en) * | 2017-12-13 | 2018-05-04 | 河南师范大学 | The preparation method and application of p-hydroxybenzoic acid trace electrochemical sensor based on nanometer modified by silver |
| CN108152346A (en) * | 2017-12-13 | 2018-06-12 | 河南师范大学 | The preparation method and application of norfloxacin molecular imprinted electrochemical sensor based on nanometer modified by silver |
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| CN101759678A (en) * | 2009-11-20 | 2010-06-30 | 南京泽朗医药科技有限公司 | Method for preparing kaempferol |
| CN102507697B (en) * | 2011-10-27 | 2014-05-14 | 西北师范大学 | Modified electrode based on molecular imprinted polymer membrane, electrochemistry system and application thereof |
| CN102809592B (en) * | 2012-07-13 | 2014-03-12 | 江苏大学 | Electro-polymerization preparation method of electrochemical sensor for quickly detecting EGCG (Epigallocatechin-3-Gallate) |
| CN103149268B (en) * | 2013-03-18 | 2014-09-24 | 桂林理工大学 | Method for utilizing molecularly imprinted electrochemical sensor to detect trace gibberellin A3 |
| CN103243367B (en) * | 2013-05-17 | 2016-04-27 | 天津前方科技有限公司 | The preparation method of streptavidin molecular imprinting biosensor |
| CN103926291B (en) * | 2014-05-06 | 2016-04-20 | 济南大学 | A kind of preparation method and application detecting the molecular engram sensor of apiolin |
| CN103965418B (en) * | 2014-05-08 | 2017-02-15 | 嘉兴学院 | Carbon nanotube surface molecularly imprinted polymer as well as preparation method and application thereof |
| CN104181215B (en) * | 2014-08-31 | 2016-11-02 | 济南大学 | The preparation of a kind of electropolymerization molecularly imprinted polymer hollow channel paper device and the application in pesticide residues detect immediately |
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