CN104458513A - Device for measuring 3D size and distribution of micro particles - Google Patents
Device for measuring 3D size and distribution of micro particles Download PDFInfo
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- CN104458513A CN104458513A CN201410724556.5A CN201410724556A CN104458513A CN 104458513 A CN104458513 A CN 104458513A CN 201410724556 A CN201410724556 A CN 201410724556A CN 104458513 A CN104458513 A CN 104458513A
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Abstract
The invention discloses a device for measuring the 3D size and the distribution of micro particles. The device comprises a light source, a condensing lens, a transparent cylinder channel, a video microscope, a digital camera and two plane mirror groups. High-brightness flare light emitted by the light source is condensed into parallel light by the condensing lens, the parallel light is divided into three paths, vertically penetrates through the transparent cylinder channel from different directions and projects on the video microscope, and a series of three images formed by enabling the three paths of light to pass through the video microscope are recorded by the digital camera. Transparent liquid containing to-be-measured micro particles flows in the cylinder channel, and the three images refer to images of a same part of particles photographed with different angles simultaneously. After the same particles in the three images are identified, the 3D size of the particles is obtained by virtue of two-dimensional profile comparison and recovery computing of the three images, finally the multiple particles in the series of images are counted by a computer, the 3D sizes of the particles are combined, and the 3D size distribution of the particles is obtained.
Description
Technical field
The present invention relates to a kind of optical measuring system, particularly the 3D size of molecule and distribution measuring.
Background technology
The related physical quantity utilizing optical method to measure molecule is used widely because having the advantage not contacting sample, and its measuring method has scattering method, diffraction approach, transmission beam method, image method etc.
Patent documentation CN1664501A, CN102834689A, US2011310386A1, CN102564928A, US007528959B2, JP2007298327A, WO8904472A1 etc. disclose some devices utilizing scattering method to measure grain size and distribution.Document CN101802589A is by diffraction measurement particle size.Document CN202119698U then utilizes transmission beam method to achieve counting to cell granulations.Document CN102834689A adopts image method to measure grain size, locus and brightness.Document CN20103491A measures grain size, quantity and CONCENTRATION DISTRIBUTION based on image method.Document CN102692364A is then based on dynamic particle measurement space position and the size of fuzzy image processing.
Above-mentionedly patent document discloses some devices measuring grain size and distribution, due to the general aspherical of particle, so this size typically refers to the equivalent volume bulb diameter value of particle or Stoke diameter or area volume diameter d
32, but be the three-dimensional dimension size which kind of diameter all can not reflect particle.About the detection of aspherical particle, document CN102323191A, WO8904472A1, CN102519850A disclose some detection methods, but these documents also only have detected the shape rate of particle, i.e. length breadth ratio, can not provide the three-dimensional dimension of particle.The two dimensional image of particle can be detected as the microscope of traditional non-automated or the observational technique of electron microscope, thus measure the two-dimensional of particle, but the three-dimensional dimension of particle can not be obtained.For this reason, the present invention aims to provide the measurement mechanism of a kind of molecule three-dimensional dimension and distribution thereof.
In prior art, the microscope of traditional non-automated or electron microscope method can only detect two-dimensional shapes and the two-dimensional of molecule, the size that can only detect particle of existing light scattering method and distribution or shape rate, existing image method can only detect the size of particle, locus and brightness, and these technology all can not provide the three-dimensional dimension of particle.And the present invention can measure 3D size and the distribution thereof of molecule, and there is good accuracy.
Summary of the invention
The object of the invention is to: the defect overcoming above-mentioned prior art, propose a kind of 3D size and apparatus for measuring distribution thereof of molecule, effective especially to the measurement of molecule 3D size.
In order to achieve the above object, the 3D size of a kind of molecule that the present invention proposes and apparatus for measuring distribution thereof, composition comprises:
Light source is high strength xenon flashing light light source, for sending the flash of light of high brightness;
Collector lens, is directional light for the light optically focused sent by light source, and penetrates;
Transparent cylinder passage, diameter 1.5 ~ 2.00 mm, inside is full of the transparency liquid being mixed into candidate particles flowed vertically, the axes normal of described directional light and transparent cylinder passage;
Videomicroscopy, is positioned at the side of transparent cylinder passage away from light source, and just to described directional light, amplifies the image of candidate particles for the light that receives through transparent cylinder passage;
Digital camera, the particle image for the amplification to described videomicroscopy completes imaging;
First, second level crossing group, for carrying out reflection commutation to directional light, comprises two level crossings being positioned at transparent cylinder passage both sides respectively;
First via light in directional light is directly through directive videomicroscopy after transparent cylinder passage; Second road light forms the light vertical with described first via light after a level crossing of the first level crossing group, vertically through transparent cylinder passage, and by another level crossing by vertically directive videomicroscopy after the light reflection that appears; It is the light of 45 ° of angles that 3rd road light is formed with described first via light after a level crossing of the second level crossing group, vertically through transparent cylinder passage, and by another level crossing by vertically directive videomicroscopy after the light reflection that appears; Imaging is completed through videomicroscopy is subregional in digital camera after three road light therethrough transparent cylinder passages.
The 3D size of molecule of the present invention and apparatus for measuring distribution thereof, also have following further feature:
1, two pieces of level crossings of described first level crossing group are arranged on the direction vertical with directional light, and lay respectively at transparent cylinder passage both sides; Two pieces of level crossings of described second level crossing group lay respectively at the outside of two pieces of level crossings of the first level crossing group, and one of them is near collector lens, and another is near videomicroscopy.
2, two pieces of level crossings of described first level crossing group respectively arrange one piece for blocking the baffle plate of directional light near transparent cylinder passage side.Prevent parasitic light from entering video camera.
3,2 shading strips are set, for splitting the imaging region of three road light before described videomicroscopy.On egative film, form 2 blank fillet regions like this, and separate 3 images with this, avoid obscuring in Digital Image Processing phase.
In this device, molecule flows through transparent cylindrical passage along with transparency liquid (as pure water), the high-luminance light that xenon flash lamp light source sends forms the side of directional light vertical incidence to transparent cylindrical fluid passage after collector lens optically focused, wherein 1 Guang Yanyuan direction, road (optical axis) is directly mapped to particle, another 1 road light after flat mirror reflects with the direction directive particle in 90 ° with optical axis, 3rd tunnel is as reference light edge directive particle at 45 ° with optical axis, after this 3 road light transmission particle, be mapped to videomicroscopy with great visual angle and form the partial particulate image amplified, digital camera can record a series of not partial particulate image in the same time, the image in certain moment reflects the image of the same partial particulate of 3 different angles, adopt simple separation setting these 3 images can be separated.Often open the image that image have recorded the same partial particulate flowed all simultaneously, particle two-dimensional silhouette image comparatively clearly can be obtained by simple binary conversion treatment, then the same particle in 3 images is gone out by pairing identifiable design, again through comparing and recover to calculate the 3D size of particle to the two-dimensional silhouette of 3 images, because the liquid in right cylinder passage is flowing, so the image series do not recorded in the same time includes the information of numerous variable grain, then by obtaining particle 3D Size Distribution to the statistics of particle multiple in these image series.The measurement of the present invention to particle 3D size has higher degree of accuracy.
The invention has the beneficial effects as follows:
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further illustrated.
Fig. 1 is the stereographic map of this measurement mechanism.
Fig. 2 is the vertical view of this measurement mechanism.
Fig. 3 is that video camera is at the 3 width image schematic diagram of synchronization from 3 different angles records.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
As shown in Figure 1 and Figure 2, for 3D size and the apparatus for measuring distribution thereof of molecule of the present invention, composition comprises: (the first level crossing group is made up of the first level crossing 4 and the second level crossing 7 for light source 1, collector lens 2, transparent cylinder passage 13, videomicroscopy 11, digital camera 12, two groups of level crossing groups, second level crossing group is made up of the 3rd level crossing 3 and the 4th level crossing 8), videomicroscopy 11 and digital camera can adopt Lycra auxiliary products (as ZA-APO videomicroscopy and DFC420 digital camera).
Wherein, light source 1 is high strength xenon flashing light light source, for sending the flash of light of high brightness; Collector lens 2, is directional light for the light optically focused sent by light source, and penetrates; Transparent cylinder passage 13, diameter 1.5 ~ 2.00 mm, inside is full of the pure water (also can be other transparency liquids) being mixed into candidate particles flowed vertically, the axes normal of directional light and transparent cylinder passage; Videomicroscopy 11 is positioned at the side of transparent cylinder passage 13 away from light source 1, and just to directional light, amplifies the image of candidate particles for the light that receives through transparent cylinder passage 13; This digital camera 12, the particle image for the amplification to videomicroscopy 11 completes imaging; First, second level crossing group, for carrying out reflection commutation to directional light, comprises two level crossings being positioned at transparent cylinder passage both sides respectively.
First via light in directional light is directly through directive videomicroscopy 11 after transparent cylinder passage 13; Second road light forms the light vertical with first via light after the first level crossing 4, vertically through transparent cylinder passage 13, and by the second level crossing 7 by vertically directive videomicroscopy 11 after the light reflection that appears; It is the light of 45 ° of angles that 3rd road light is formed with first via light after the 3rd level crossing 3, vertically through transparent cylinder passage 13, and by the 4th level crossing 8 by vertically directive videomicroscopy 11 after the light reflection that appears; Imaging is completed through videomicroscopy is subregional in digital camera after three road light therethrough transparent cylinder passages.
As shown in Figure 2, two pieces of level crossings (the first level crossing 4, second level crossing 7) of the first level crossing group are arranged in directional light (first via light) vertical direction, and lay respectively at transparent cylinder passage 13 both sides; Two pieces of level crossings (the 3rd level crossing 3, the 4th level crossing 8) of the second level crossing group lay respectively at the outside of two pieces of level crossings (the first level crossing 4, second level crossing 7) of the first level crossing group, wherein the 3rd level crossing 3 is near collector lens 2, and the 4th level crossing 8 is near videomicroscopy 11.
The high brightness flash of light that high strength xenon flashing light light sends, is directional light by optically focused after collector lens 2, projects vertically towards transparent cylinder passage 13.This directional light is become 3 tunnels by first, second level crossing component, wherein first via light along optical axis direction directly through right cylinder passage 13; Second road light, by forming the light in 90 ° with the first via after the first level crossing 4, after right cylinder passage 13, then forms the light identical with optical axis direction through the second level crossing 7; 3rd road light, by forming the light at 45 ° with the first via after the 4th level crossing 3, after right cylinder passage 13, then forms the light identical with optical axis direction through the 4th level crossing 8.Digital camera 12 can record a series of by the 3 width image (see figure 3)s of above-mentioned 3 road light by being formed after a videomicroscopy 11.In right cylinder passage 13, flowing is the transparency liquid containing molecule to be measured, so this 3 width image is exactly the image of the same partial particulate taken the photograph from different perspectives simultaneously.Particle two-dimensional silhouette image comparatively clearly can be obtained by simple binary conversion treatment, then by matching the same particle that can automatically identify in 3 images, again through comparing and recover to calculate the 3D size of particle to the two-dimensional silhouette of 3 images, last by the statistics of computing machine to the multiple particles in image series, in conjunction with the 3D size of each particle, obtain the 3D Size Distribution of particle.
2 shading strips 9,10 are set before videomicroscopy, for splitting the imaging region of three road light.The image taken the photograph at video camera like this forms 2 blank fillet regions, separates 3 images with this, avoid obscuring in Digital Image Processing phase.
In order to prevent parasitic light from entering video camera, two pieces of level crossings of the first level crossing group arrange the first baffle plate 5 for blocking directional light and second baffle 6 respectively near transparent cylinder passage side.
Fig. 3 is the image schematic diagram that video camera is taken the photograph at certain, this image contains separate 3 width images 21,22 and 23 longitudinally, after digital technology process, every width image clearly shows the image of 5 particles, the particles effect 31 wherein in image 21 and the particles effect 32 in image 22 and all corresponding same particle of the particles effect 33 in image 23.
The two-dimensional silhouette of same particle in different images is compared and recovers can be calculated the length of this particle, be i.e. 3D size.Again by adding up multiple particles of image series record, obtain particle 3D Size Distribution further.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of application claims.
Claims (4)
1. the 3D size of molecule and an apparatus for measuring distribution thereof, composition comprises:
Light source is high strength xenon flashing light light source, for sending the flash of light of high brightness;
Collector lens, is directional light for the light optically focused sent by light source, and penetrates;
Transparent cylinder passage, diameter 1.5 ~ 2.00 mm, inside is full of the transparency liquid being mixed into candidate particles flowed vertically, the axes normal of described directional light and transparent cylinder passage;
Videomicroscopy, is positioned at the side of transparent cylinder passage away from light source, and just to described directional light, amplifies the image of candidate particles for the light that receives through described transparent cylinder passage;
Digital camera, the particle image for the amplification to described videomicroscopy completes imaging;
First, second level crossing group, for carrying out reflection commutation to directional light, comprises two level crossings being positioned at transparent cylinder passage both sides respectively;
First via light in directional light is directly through directive videomicroscopy after transparent cylinder passage; Second road light forms the light vertical with described first via light after a level crossing of the first level crossing group, vertically through transparent cylinder passage, and by another level crossing by vertically directive videomicroscopy after the light reflection that appears; It is the light of 45 ° of angles that 3rd road light is formed with described first via light after a level crossing of the second level crossing group, vertically through transparent cylinder passage, and by another level crossing by vertically directive videomicroscopy after the light reflection that appears; Imaging is completed through videomicroscopy is subregional in digital camera after three road light therethrough transparent cylinder passages.
2. the 3D size of molecule according to claim 1 and apparatus for measuring distribution thereof, is characterized in that: two pieces of level crossings of described first level crossing group are arranged on the direction vertical with directional light, and lay respectively at transparent cylinder passage both sides; Two pieces of level crossings of described second level crossing group lay respectively at the outside of two pieces of level crossings of the first level crossing group, and one of them is near collector lens, and another is near videomicroscopy.
3. the 3D size of molecule according to claim 1 and apparatus for measuring distribution thereof, is characterized in that: two pieces of level crossings of described first level crossing group respectively arrange one piece for blocking the baffle plate of directional light near transparent cylinder passage side.
4. the 3D size of molecule according to claim 3 and apparatus for measuring distribution thereof, is characterized in that: arrange 2 shading strips before described videomicroscopy, for splitting the imaging region of three road light.
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Cited By (3)
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| CN109146949A (en) * | 2018-09-05 | 2019-01-04 | 天目爱视（北京）科技有限公司 | A kind of 3D measurement and information acquisition device based on video data |
| KR102098701B1 (en) * | 2019-03-12 | 2020-04-08 | 주식회사 지씨에스월드 | Apparatus for detecting dust and analyzing shape thereof in liquid using image sensor and method thereof |
| CN114166699A (en) * | 2021-11-12 | 2022-03-11 | 清华大学深圳国际研究生院 | Optical measurement device and method for volume of suspended particles |
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| CN114166699A (en) * | 2021-11-12 | 2022-03-11 | 清华大学深圳国际研究生院 | Optical measurement device and method for volume of suspended particles |
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