CN104447493B - Method for extracting L-tryptophan high-efficiency membrane - Google Patents
Method for extracting L-tryptophan high-efficiency membrane Download PDFInfo
- Publication number
- CN104447493B CN104447493B CN201410666529.7A CN201410666529A CN104447493B CN 104447493 B CN104447493 B CN 104447493B CN 201410666529 A CN201410666529 A CN 201410666529A CN 104447493 B CN104447493 B CN 104447493B
- Authority
- CN
- China
- Prior art keywords
- storage tank
- tryptophan
- filter
- tank
- fermentation liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
- C07D209/20—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals substituted additionally by nitrogen atoms, e.g. tryptophane
Abstract
The invention discloses a method for extracting an L-tryptophan high-efficiency membrane. The method comprises the following steps of: firstly transferring L-tryptophan fermentation liquor which is already fermented into a fermentation liquor storage tank, then delivering the fermentation liquor into a ceramic membrane filter for filtration, controlling the flow velocity of filter liquor in a filtering process so that the trans-membrane pressure difference of a ceramic membrane is not more than 0.4MPa, and delivering filtered clear tryptophan liquor into a clear liquor tank for preservation and later use; when the content of the fermentation liquor inside the fermentation liquor storage tank is less than 4%, stopping filtering, and leaving fungi residues inside the fermentation liquor storage tank for later use; placing the fungi residues inside the fermentation liquor storage tank into a fungi residue tank, then adding a flocculant to the fungi residue tank, placing into a plate and frame filter press after mixed residues are in a viscous state, and carrying out plate-frame pressure filtration to obtain a filter cake and filter liquor, wherein the filter cake is solid protein, and the filter liquor is returned into the fermentation liquor storage tank as dialysis water for reutilization. The method disclosed by the invention can be used for effectively reducing the operation time of the ceramic membrane, increasing the fermentation tanking batch, reducing the production cost and reducing the discharge amount of sewage.
Description
Technical field
The invention belongs to aminoacid extractive technique field, relate generally to a kind of l- tryptophan high performance membrane extracting method.
Background technology
At present, tryptophan extract be limited by unit produce acid low, in fermentation liquid impurity larger is difficult extract, therefore post yield
Most important lead to ceramic membrane run time longer it is ensured that ceramic membrane yield needs to add a large amount of water-dialyzings to be diluted, system
The about upper tank batch of fermentation, the serious more difficult thoroughly cleaning of fouling membrane simultaneously, sewage flow rate is larger.
Content of the invention
The purpose of the present invention is to extract difficulty, ceramic membrane long operational time, row for tryptophan present in prior art
The technical problem such as big is measured in dirt, provides a kind of l- tryptophan high performance membrane extracting method, and the method can effectively reduce ceramic membrane and run
Time, improve the upper tank batch of fermentation, reduce production cost, reduce quantity of wastewater effluent.
The present invention realizes above-mentioned purpose and the technical scheme is that a kind of l- tryptophan high performance membrane extracting method, including with
Lower step: the l- tryptophan fermentation liquid first completing fermentation is transferred in fermentation liquid storage tank, then in fermentation liquid storage tank continuously
Water-dialyzing is added to be diluted, the concentration being diluted to l- tryptophan is for, after 14-20g/l, while continuing dilution, starting to send out
Zymotic fluid is delivered in purpose ceramic-film filter and is filtered, and when the concentration of l- tryptophan in the liquid storage tank that ferments is 8g/l, stops
Dilution；In filter process, the flow velocity of filtrate is controlled to make the transmembrane pressure difference of ceramic membrane be less than 0.4mpa, through the l- filtering
Tryptophan clear liquid is delivered in Clear liquid tank and preserves, standby；When fermentation liquid storage tank in fermentation liquid content reach original amount 4% with
Stop when lower filtering, be bacteria residue in fermentation liquid storage tank, standby；
Bacteria residue in fermentation liquid storage tank is squeezed into bacteria residue tank by centrifugal pump, then into bacteria residue tank, adds flocculation agent, treat
Mixing material residue in bacteria residue tank becomes thick and squeezes into filter press by centrifugal pump afterwards, obtains filter cake and filter after filter press
Liquid, filter cake is solid protein, and filtrate is recycled to fermentation liquid storage tank recycling as water-dialyzing.
Described flocculation agent is aluminium polychlorid.
The addition of described flocculation agent is the 0.08-0.3% of bacteria residue weight.
Beneficial effects of the present invention
The l- tryptophan high performance membrane extracting method that the present invention provides passes through to control the concentration and pressure during ceramic membrane filter, energy
Enough effectively minimizing ceramic membrane run times, improve the upper tank batch of fermentation, save power consumption, and do not affect product yield；Bacteria residue warp
Cross the solid protein obtaining after processing to use directly as albumen feedstuff, filtrate can reuse as water-dialyzing, subtracts
Few water consumption, reduces production cost, reduces quantity of wastewater effluent.
Brief description
Fig. 1 is the process route view of the present invention.
Specific embodiment
A kind of l- tryptophan high performance membrane extracting method, comprises the following steps: the l- tryptophan fermentation liquid first completing fermentation
It is transferred in fermentation liquid storage tank, then in fermentation liquid storage tank, be continuously added to water-dialyzing and be diluted, be diluted to l- tryptophan
After concentration is 14-20g/l, while continuing dilution, starts fermentation liquid is delivered in purpose ceramic-film filter and filtered, and
When the concentration of l- tryptophan in the liquid storage tank that ferments is 8g/l, stop dilution；In filter process, the flow velocity of filtrate is controlled to make pottery
The transmembrane pressure difference of porcelain film is less than 0.4mpa, and the l- tryptophan clear liquid through filtering is delivered in Clear liquid tank and preserves, standby；When
Fermentation liquid storage tank in fermentation liquid content reach original amount below 4% when stop filter, fermentation liquid storage tank in be bacteria residue, standby；
Bacteria residue in fermentation liquid storage tank is squeezed into bacteria residue tank by centrifugal pump, then into bacteria residue tank, adds flocculation agent, treat
Mixing material residue in bacteria residue tank becomes thick and squeezes into filter press by centrifugal pump afterwards, obtains filter cake and filter after filter press
Liquid, filter cake is solid protein, and filtrate is recycled to fermentation liquid storage tank recycling as water-dialyzing.
Described flocculation agent is aluminium polychlorid.
The addition of described flocculation agent is the 0.08-0.3% of bacteria residue weight.
Below in conjunction with specific embodiment, the present invention will be further described:
Embodiment 1:
A kind of l- tryptophan high performance membrane extracting method, comprises the following steps: the l- tryptophan fermentation liquid first completing fermentation
It is transferred in fermentation liquid storage tank, then in fermentation liquid storage tank, be continuously added to water-dialyzing and be diluted, be diluted to l- tryptophan
After concentration is 14g/l, while continuing dilution, starts fermentation liquid is delivered in purpose ceramic-film filter and filtered, and work as
When the concentration of l- tryptophan is 8g/l in fermentation liquid storage tank, stop dilution；In filter process, the flow velocity of filtrate is controlled to make pottery
The transmembrane pressure difference of film is less than 0.4mpa, and the l- tryptophan clear liquid through filtering is delivered in Clear liquid tank and preserves, standby；When send out
The content of the fermentation liquid in zymotic fluid storage tank reach original amount below 4% when stop filter, fermentation liquid storage tank in be bacteria residue, standby；
Bacteria residue in fermentation liquid storage tank is squeezed into bacteria residue tank by centrifugal pump, into bacteria residue tank, then adds flocculation agent, institute
The flocculation agent stated is aluminium polychlorid, and its addition is the 0.3% of bacteria residue weight；Treat that the mixing material residue in bacteria residue tank becomes thick
Afterwards filter press is squeezed into by centrifugal pump, obtain filter cake and filtrate after filter press, filter cake is solid protein, filtrate conduct
Water-dialyzing is recycled to fermentation liquid storage tank recycling.
Embodiment 2:
A kind of l- tryptophan high performance membrane extracting method, comprises the following steps: the l- tryptophan fermentation liquid first completing fermentation
It is transferred in fermentation liquid storage tank, then in fermentation liquid storage tank, be continuously added to water-dialyzing and be diluted, be diluted to l- tryptophan
After concentration is 20g/l, while continuing dilution, starts fermentation liquid is delivered in purpose ceramic-film filter and filtered, and work as
When the concentration of l- tryptophan is 8g/l in fermentation liquid storage tank, stop dilution；In filter process, the flow velocity of filtrate is controlled to make pottery
The transmembrane pressure difference of film is less than 0.4mpa, and the l- tryptophan clear liquid through filtering is delivered in Clear liquid tank and preserves, standby；When send out
The content of the fermentation liquid in zymotic fluid storage tank reach original amount below 4% when stop filter, fermentation liquid storage tank in be bacteria residue, standby；
Bacteria residue in fermentation liquid storage tank is squeezed into bacteria residue tank by centrifugal pump, into bacteria residue tank, then adds flocculation agent, institute
The flocculation agent stated is aluminium polychlorid, and its addition is the 0.08% of bacteria residue weight；Treat that the mixing material residue in bacteria residue tank becomes thick
Afterwards filter press is squeezed into by centrifugal pump, obtain filter cake and filtrate after filter press, filter cake is solid protein, filtrate conduct
Water-dialyzing is recycled to fermentation liquid storage tank recycling.
Embodiment 3:
A kind of l- tryptophan high performance membrane extracting method, comprises the following steps: the l- tryptophan fermentation liquid first completing fermentation
It is transferred in fermentation liquid storage tank, then in fermentation liquid storage tank, be continuously added to water-dialyzing and be diluted, be diluted to the dense of tryptophan
After spending for 17g/l, while continuing dilution, start fermentation liquid is delivered in purpose ceramic-film filter and filtered, and ought send out
When the concentration of tryptophan is 8g/l in zymotic fluid storage tank, stop dilution；In filter process, the flow velocity of filtrate is controlled to make ceramic membrane
Transmembrane pressure difference is less than 0.4mpa, and the tryptophan clear liquid through filtering is delivered in Clear liquid tank and preserves, standby；When fermentation liquid storage
The content of the fermentation liquid in tank reach original amount below 4% when stop filter, fermentation liquid storage tank in be bacteria residue, standby；
Bacteria residue in fermentation liquid storage tank is squeezed into bacteria residue tank by centrifugal pump, into bacteria residue tank, then adds flocculation agent, institute
The flocculation agent stated is aluminium polychlorid, and its addition is the 0.18% of bacteria residue weight；Treat that the mixing material residue in bacteria residue tank becomes thick
Afterwards filter press is squeezed into by centrifugal pump, obtain filter cake and filtrate after filter press, filter cake is solid protein, filtrate conduct
Water-dialyzing is recycled to fermentation liquid storage tank recycling.
Claims (3)
1. a kind of l- tryptophan high performance membrane extracting method it is characterised in that: comprise the following steps: first by the l- color ammonia that completes of fermentation
Acid fermentation liquid is transferred in fermentation liquid storage tank, is then continuously added to water-dialyzing in fermentation liquid storage tank and is diluted, is diluted to l-
After the concentration of tryptophan is 14-20g/l, while continuing dilution, start to be delivered in purpose ceramic-film filter by fermentation liquid
Row filters, and when the concentration of l- tryptophan in the liquid storage tank that ferments is 8g/l, stops dilution；In filter process, control filtrate
Flow velocity make ceramic membrane transmembrane pressure difference be less than 0.4mpa, through filtration l- tryptophan clear liquid be delivered in Clear liquid tank protect
Deposit, standby；When ferment liquid storage tank in fermentation liquid content reach original amount below 4% when stop filter, fermentation liquid storage tank in be
Bacteria residue, standby；
Bacteria residue in fermentation liquid storage tank is squeezed into bacteria residue tank by centrifugal pump, then into bacteria residue tank, adds flocculation agent, treat bacteria residue
Mixing material residue in tank becomes thick and squeezes into filter press by centrifugal pump afterwards, obtains filter cake and filtrate after filter press,
Filter cake is solid protein, and filtrate is recycled to fermentation liquid storage tank recycling as water-dialyzing.
2. as claimed in claim 1 a kind of l- tryptophan high performance membrane extracting method it is characterised in that: described flocculation agent is poly-
Close aluminum chloride.
3. as claimed in claim 1 or 2 a kind of l- tryptophan high performance membrane extracting method it is characterised in that: described flocculation agent
Addition be bacteria residue weight 0.08-0.3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410666529.7A CN104447493B (en) | 2014-11-20 | 2014-11-20 | Method for extracting L-tryptophan high-efficiency membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410666529.7A CN104447493B (en) | 2014-11-20 | 2014-11-20 | Method for extracting L-tryptophan high-efficiency membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104447493A CN104447493A (en) | 2015-03-25 |
| CN104447493B true CN104447493B (en) | 2017-01-18 |
Family
ID=52894326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410666529.7A Active CN104447493B (en) | 2014-11-20 | 2014-11-20 | Method for extracting L-tryptophan high-efficiency membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104447493B (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101307301A (en) * | 2007-05-17 | 2008-11-19 | 浙江升华拜克生物股份有限公司 | L-tryptophan genetic engineering bacterium and process for producing L-tryptophan |
| CN101376646A (en) * | 2007-08-29 | 2009-03-04 | 天津科技大学 | Novel method for extracting L-tryptophan from fermentation liquor |
-
2014
- 2014-11-20 CN CN201410666529.7A patent/CN104447493B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104447493A (en) | 2015-03-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102030698B (en) | Method for separating and extracting L-tryptophan in fermentation liquor by utilizing organic film | |
| CN103626836A (en) | Extraction process for polypeptide from waste liquid obtained in production of crude heparin sodium product | |
| CN207002435U (en) | The recycle device of semicon industry abrasive waste water | |
| CN104447493B (en) | Method for extracting L-tryptophan high-efficiency membrane | |
| CN202590632U (en) | Inorganic tube type ceramic membrane fine filtration system | |
| CN101423307B (en) | Method for recycling spent caustic liquid during chitin production process | |
| CN203353611U (en) | Soy sauce filtering system | |
| CN206298538U (en) | A kind of plant polyose processing device | |
| CN101974381B (en) | Technology for recycling beer from beer waste yeast paste | |
| CN206103401U (en) | Vegetable protein draws concentrator | |
| CN104478786B (en) | A kind of L-Trp film extracts energy conserving system | |
| CN206934796U (en) | A kind of normal temperature concentrator of rose extract | |
| CN108477470B (en) | Tank type high-precision low-energy-consumption full-automatic desalination system and desalination method | |
| CN204298291U (en) | A kind of L-Trp film extracts energy saver | |
| CN202829636U (en) | Device for processing reverse osmosis concentrated brine by using decompressing membrane distillation | |
| CN207775032U (en) | Electroplating wastewater zero processing unit | |
| CN102786164A (en) | System for settlement-free physicochemical treatment on water-based ink waste water | |
| CN105036245A (en) | Medium-temperature biogas slurry wastewater pipe-type ultrafiltration membrane system treatment and recycling technique | |
| CN204714614U (en) | Swimming paint filtering recovering system | |
| CN205145993U (en) | A equipment for traditional chinese medicine purification | |
| CN204619755U (en) | A kind of Novel ultrafiltration device | |
| CN204702718U (en) | A kind of dregs of beans leaches workshop hybrid oil filter device | |
| CN204689792U (en) | A kind of wastewater treatment equipment of Zero-discharge non-pollution | |
| CN204824544U (en) | Waste water clean system | |
| CN212790073U (en) | Low salt soy sauce membrane filtration coupling system of processing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |