CN104436193B - Preparation method of folic acid coupled gold nano-rod/polypyrrole/ferroferric oxide multifunctional composite nano diagnosis and treatment agent - Google Patents
Preparation method of folic acid coupled gold nano-rod/polypyrrole/ferroferric oxide multifunctional composite nano diagnosis and treatment agent Download PDFInfo
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Abstract
The invention relates to a preparation method of a folic acid coupled gold nano-rod/polypyrrole/ferroferric oxide multifunctional composite nano diagnosis and treatment agent. The preparation method comprises the following steps: by adopting composite nanoparticles consisting of gold nano-rods, polypyrrole and ferroferric oxide as a substrate, modifying the surfaces of the composite nanoparticles by using polycationic electrolyte chitosan and polyanionic electrolyte sodium alginate respectively by virtue of a layer-by-layer self-assembly process to ensure that the surfaces of the nanoparticles contain amino groups, and then connecting with carboxy groups of folic acid. The prepared multifunctional composite nano diagnosis and treatment agent disclosed by the invention has the advantages of high photo-thermal conversion efficiency, good biocompatibility, active tumor targeting performance, strong cancer cell killing ability, significant imaging effect, a function of being controlled for a long time, and the like, and can be applied to treatment, pathological magnetic resonance imaging analysis and medical diagnosis of X-ray computer tomography and ultrasonic imaging of tumors or cancers of human bodies or other mammals.
Description
Technical field
The invention belongs to nanometer diagnosis and treatment agent field, particularly to a kind of folacin coupled gold nanorods/polypyrrole/tetra- oxidation
The preparation method of three-iron Multifunctional composite nanometer diagnosis and treatment agent.
Background technology
Tumor has become as and leads to one of principal disease of human death, and the tumor patient number made a definite diagnosis every year of the whole world is with thousand
Ten thousand, die from up to million person-times of the patient of tumor.At present still continue to use the means such as diagnosis, the radiation and chemotherapy of early stage and carry out tumor and control
Treat.To a certain extent, although traditional chemotherapy of tumors method can play the effect for the treatment of tumor, due to lacking targeting
Property, with the hypoimmunity that deeply can lead to patient for the treatment of, this brings great pain to the body and mind of patient.Additionally, tumor
It is a kind of complex disease of many mechanism, the 80% tumor patient cancerous cell when being diagnosed just has occurred and that transfer.Therefore, adopt
New technique improve to the early warning of tumor, early diagnosiss, the ability of transfer monitoring and find safely, effectively with economic treatment side
Method has become clinical problem demanding prompt solution at present.
Nano material is expected to solve the problems, such as that traditional medicine method cann't be solved, this must will draw because of its unique advantage
Play modern medical diagnosis and the new change for the treatment of.Combine with the medical oncology nanometer medical oncology of formation of nanotechnology is nanometer
Emerging field in medical science, is also the core realm that current various countries frontier science and technology are first developed.Meanwhile, nano biological medical science is fast
Speed develops into safe efficient, the special, intelligence of exploitation and the nano-probe of targeting provides new thinking.
At present, the clinical diagnosises of tumor depend on two methods：(1) biological detection method, including immunology inspection
Survey, SABC, gene chip and polymerase chain reaction technology；(2) Medical Imaging method, including NMR (Nuclear Magnetic Resonance)-imaging skill
Art, X-ray Computed tomography, medicine imaging technique and ultrasonic imaging technique etc..If will there is advanced function
The nanostructured of nano material and intelligent response is applied to the imaging study of tumor cells, this and traditional in-vitro diagnosis phase
Ratio can not only be the early diagnosiss of tumor and the transfer early warning strong means of offer, but also can be the development of tumor
Journey provides more directly perceived and abundant evidence.Tumor cells image has become as the most important thing in nanometer medical oncology research.
On the basis of tumor cells image, in conjunction with therapeutic methods such as medicine, light power or photo-thermal, collection diagnosis will be createed and control
Treat the multifunctional nano diagnosis and treatment agent in one, this has become current Nanomolecular imaging and the research of nano biological medical domain
Focus.
Content of the invention
The present invention proposes a kind of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide Multifunctional composite nanometer and examines
Treat the preparation method of agent, the method apparatus is simple, easy and simple to handle, contributes to industrialized production；The composite Nano being obtained
Diagnosis and treatment agent good dispersion, good stability, uniform particle diameter, and there is good biocompatibility and targeting.
A kind of folacin coupled gold nanorods/polypyrrole of the present invention/ferroso-ferric oxide Multifunctional composite nanometer diagnosis and treatment agent
Preparation method, including：
(1) prepare the stable gold nanorods solution of surfactant；
(2) ferric chloride is added in poly-vinyl alcohol solution, heating is sufficiently stirred for, is configured to polymer solution；
(3) the gold nanorods solution preparing step (1) is added in polymer solution prepared by step (2), ultrasonic point
Dissipate, stir；
(4) under condition of ice bath, pyrrole monomer is added dropwise in mixed solution prepared by step (3), stirring 10～
After 60min, it is placed in low temperature environment, keeps 3～24h, treat that solution becomes black, be obtained and be combined containing gold nanorods/polypyrrole
The suspension of nanoparticle；
(5) suspension of the gold nanorods/polypyrrole nanoparticle preparing step (4), deionized water and ethanol are dilute
Release, rise high-temperature, under nitrogen protection, add ammonia spirit, after reaction 10～40min, add ammonia spirit again, continue anti-
Answer 10～40min, obtain gold nanorods/polypyrrole/ferroso-ferric oxide composite Nano diagnosis and treatment agent, by externally-applied magnetic field, collect grain
Son；
(6) cationic polyelectrolyte chitosan solution and anionic polyelectrolyte sodium alginate soln are prepared, then
It is added separately to successively in particle solution prepared by step (5), centrifuge washing disperses, then is separately added into shitosan and sodium alginate
Solution, stirs 0.2～2 hour, and centrifuge washing disperses, and circulates 3～5 times, i.e. LBL self-assembly；
(7) will be molten to N-Hydroxysuccinimide, N- (3- dimethylamino-propyl)-N'- ethyl-carbodiimide hydrochloride and Folic Acid
In deionized water；
(8) nanoparticle prepared by collection step (6), dispersion in deionized water, is added in step (7) solution, uses
Triethylamine makees catalyst, after adjusting pH, stirs 2～24h, washing is collected by centrifugation, obtains final product folacin coupled gold nanorods/poly- pyrrole
Cough up/ferroso-ferric oxide Multifunctional composite nanometer diagnosis and treatment agent.
In described step (2), the concentration of poly-vinyl alcohol solution is 5～10%, the ratio of ferric chloride and poly-vinyl alcohol solution
For 100～500mg:10ml.
In described step (3), gold nanorods solution and the volume ratio of polymer solution are 1:5～10.
In described step (4), pyrrole monomer and the volume ratio of gold nanorods solution are 0.025～0.1:1.
In described step (4), the temperature of low temperature environment is 1～5 DEG C.
In described step (5), the suspension vol of gold nanorods/polypyrrole nanoparticle is 1～5mL, deionized water and second
Alcohol volume ratio 5～8:1, the cumulative volume after suspension dilution is 5～30mL；The ammonia spirit adding twice and the volume of suspension
Ratio respectively 0.2～2:5～30.
In described step (5), temperature is increased to 50～80 DEG C.
In described step (6), the concentration of chitosan solution and sodium alginate soln is 5～20mg/mL.
N-Hydroxysuccinimide, N- (3- dimethylamino-propyl)-N'- ethyl carbodiimide salt is added in described step (7)
Hydrochlorate and Folic Acid are respectively 0.1～0.5g, 0.1～0.5g and 0.05～0.5g.
In described step (8), triethylamine and the volume ratio of deionized water are 1～5:3～30；Adjust pH scope be 7.0～
9.0.
The folacin coupled Multifunctional composite nanometer diagnosis and treatment agent of present invention preparation has good biocompatibility, tumor-targeting
By force, imaging effect is good and can be controlled for a long time the advantages of, can be used for various tumors or the cancer of human body or other mammals
The diagnosis of disease, treatment and pathological changes analysis.
The folacin coupled Multifunctional composite nanometer diagnosis and treatment agent of present invention preparation is a kind of to integrate the compound of many performances
Nanometer diagnosis and treatment agent, its material of main part is that polypyrrole can absorb substantial amounts of 808nm near infrared light, efficiently can be changed into luminous energy
Heat energy, and by centralized heat energy in tumor sites, effectively tumor cell is killed.Simultaneously as containing in composite nanoparticle
NMR (Nuclear Magnetic Resonance)-imaging, CT thus can be imaged and photothermal imaging by the composition such as gold nanorods and SPIO
Combine the image producing high resolution.Modified with folic acid is passed through on surface, can make diagnosis and treatment agent active targeting to tumor.This is combined and receives
Rice diagnosis and treatment agent can not only be the early diagnosiss of disease and evolution provides more intuitive evidence, and is expected to realize image
The targets such as the medicine transmission of mediation, excision, the therapeutic response of monitor in real time.
Beneficial effect
(1) experimental provision according to the present invention is simple, simple to operate, and manufacturing cycle is fast, is conducive to producing in enormous quantities；
(2) the folacin coupled Multifunctional composite nanometer diagnosis and treatment agent of present invention preparation has photo-thermal therapy, NMR (Nuclear Magnetic Resonance) imaging, CT
The features such as imaging and photothermal imaging, realize the diagnosis and treatment integration of tumor；
(3) the folacin coupled Multifunctional composite nanometer diagnosis and treatment agent of present invention preparation, targeting is strong, and specific aim is high, can have
Hope the therapeutic effect improving tumor；
(4) the folacin coupled Multifunctional composite nanometer diagnosis and treatment agent of present invention preparation has good biocompatibility, stability
Height, the features such as susceptiveness is strong.
Brief description
Fig. 1 is that folacin coupled multi-functional being combined of gold nanorods/polypyrrole/ferroso-ferric oxide prepared by embodiment 1 is received
The transmission electron microscope photo of rice diagnosis and treatment agent；
Fig. 2 is the NMR (Nuclear Magnetic Resonance) imaging design sketch of the compound diagnosis and treatment agent prepared by embodiment 1；
Fig. 3 is the CT imaging effect figure of the compound diagnosis and treatment agent prepared by embodiment 1；
Fig. 4 is the photothermal deformation curve of the compound diagnosis and treatment agent prepared by embodiment 1；
Fig. 5 is that the gold nanorods/polypyrrole composite nanoparticle scanning electron microscope of the compound diagnosis and treatment agent prepared by embodiment 2 shines
Piece；
Fig. 6 is the gold nanorods/polypyrrole/ferroso-ferric oxide composite nano-granule of the compound diagnosis and treatment agent prepared by embodiment 2
Sub- stereoscan photograph；
Fig. 7 is the X ray diffracting spectrum of the compound diagnosis and treatment agent prepared by embodiment 2.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than restriction the scope of the present invention.In addition, it is to be understood that after having read the content of present invention instruction, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
(1) prepare the stable gold nanorods solution of sodium lauryl sulphate；
(2) 0.373g ferric chloride is added in 10mL 8% poly-vinyl alcohol solution, heating is sufficiently stirred for；
(3) 1mL gold nanorods solution prepared by step (1) is added in polymer solution prepared by step (2), ultrasonic
Dispersion, stirs；
(4) by the pyrrole monomer of 70 μ L, after ice bath, it is added dropwise in mixed solution prepared by step (3), stir 10min
Afterwards, it is quickly placed in 4 DEG C of refrigerators, keeps 12h, solution becomes black, be obtained outstanding containing gold nanorods/polypyrrole nanoparticle
Supernatant liquid；
(5) measure the suspension of gold nanorods/polypyrrole nanoparticle prepared by 2mL step (4), with 12mL ionized water and
1.6mL ethanol dilution, temperature is increased to 60 DEG C, under nitrogen protection, rapidly joins the ammonia spirit of 0.8mL, reacts 25min
Afterwards, again add 0.8mL ammonia spirit, continue reaction 25min, obtain gold nanorods/polypyrrole/ferroso-ferric oxide be combined receive
Rice diagnosis and treatment agent, by externally-applied magnetic field, collects particle；
(6) prepare the chitosan solution of 10mg/mL and the sodium alginate soln of 10mg/mL, then take 5mL to add respectively successively
Enter in the particle solution prepared to step (5), centrifuge washing disperses, then is separately added into 5mL shitosan and sodium alginate soln, stirs
Mix 20min, centrifuge washing disperses, this process circulates 3 times；
(7) by N- (3- the dimethylamino-propyl)-N'- ethyl carbodiimide salt of the N-Hydroxysuccinimide of 0.3g, 0.16g
Hydrochlorate and 100mg Folic Acid are dissolved in deionized water；
(8) nanoparticle 1g prepared by collection step (6), is dispersed in 20mL deionized water, is added to step (7) solution
In, make catalyst with 2mL triethylamine, after adjusting pH to 8.0, stirring 12h is collected by centrifugation washing at 37 DEG C.
Embodiment 2
(1) prepare the stable gold nanorods solution of sodium lauryl sulphate；
(2) 180mg ferric chloride is added in 10mL 8% poly-vinyl alcohol solution, heating is sufficiently stirred for；
(3) 2mL gold nanorods solution prepared by step (1) is added in polymer solution prepared by step (2), ultrasonic
Dispersion, stirs；
(4) by the pyrrole monomer of 100 μ L, after ice bath, it is added dropwise in mixed solution prepared by step (3), stir 10min
Afterwards, it is quickly placed in 4 DEG C of refrigerators, keeps 12h, solution becomes black, be obtained outstanding containing gold nanorods/polypyrrole nanoparticle
Supernatant liquid；
(5) measure the suspension of gold nanorods/polypyrrole nanoparticle prepared by 5mL step (4), with 16mL ionized water and
2mL ethanol dilution, temperature is increased to 70 DEG C, under nitrogen protection, rapidly joins the ammonia spirit of 1.2mL, after reaction 25min,
Add the ammonia spirit of 1.2mL again, continue reaction 25min, obtain gold nanorods/polypyrrole/ferroso-ferric oxide composite Nano
Diagnosis and treatment agent, by externally-applied magnetic field, collects particle；
(6) prepare the chitosan solution of 15mg/mL and the sodium alginate soln of 15mg/mL, then take 4mL to add respectively successively
Enter in the particle solution prepared to step (5), centrifuge washing disperses, then is separately added into 4mL shitosan and sodium alginate soln, stirs
Mix 30min, centrifuge washing disperses, this process circulates 3 times；
(7) by N- (3- the dimethylamino-propyl)-N'- ethyl carbodiimide salt of the N-Hydroxysuccinimide of 0.4g, 0.3g
Hydrochlorate and 150mg Folic Acid are dissolved in deionized water；
(8) nanoparticle 1.5g prepared by collection step (6), is dispersed in 20mL deionized water, is added to step (7) molten
In liquid, make catalyst with 3mL triethylamine, after adjusting pH to 9.0, stirring 12h is collected by centrifugation washing at 37 DEG C.
Claims (10)
1. the preparation method of a kind of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide Multifunctional composite nanometer diagnosis and treatment agent,
Including：
(1) prepare the stable gold nanorods solution of surfactant；
(2) ferric chloride is added in poly-vinyl alcohol solution, heating is sufficiently stirred for, is configured to polymer solution；
(3) the gold nanorods solution preparing step (1) is added in polymer solution prepared by step (2), and ultrasonic disperse stirs
Mix uniformly；
(4) under condition of ice bath, pyrrole monomer is added dropwise in mixed solution prepared by step (3), after stirring 10～60min,
It is placed in low temperature environment, keeps 3～24h, suspension containing gold nanorods/polypyrrole composite nanoparticle is obtained；
(5) suspension, deionized water and the ethanol dilution of the gold nanorods/polypyrrole nanoparticle preparing step (4), rises
High-temperature, under nitrogen protection, adds ammonia spirit, after reaction 10～40min, adds ammonia spirit again, continue reaction 10
～40min, obtains gold nanorods/polypyrrole/ferroso-ferric oxide composite Nano diagnosis and treatment agent, by externally-applied magnetic field, collects particle；
(6) prepare chitosan solution and sodium alginate soln, be then added separately to particle solution prepared by step (5) successively
In, centrifuge washing disperses, then is separately added into shitosan and sodium alginate soln, stirs 0.2～2 hour, and centrifuge washing disperses, and follows
Ring 3～5 times；
(7) N-Hydroxysuccinimide, N- (3- dimethylamino-propyl)-N'- ethyl-carbodiimide hydrochloride and Folic Acid are dissolved in
In ionized water；
(8) nanoparticle prepared by collection step (6), dispersion in deionized water, is added in step (7) solution, uses three second
Amine makees catalyst, after adjusting pH, stirs 2～24h, washing is collected by centrifugation, obtains final product folacin coupled gold nanorods/polypyrrole/tetra-
Fe 3 O Multifunctional composite nanometer diagnosis and treatment agent.
2. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：In described step (2), the concentration of poly-vinyl alcohol solution is 5～10%, tri-chlorination
The ratio of ferrum and poly-vinyl alcohol solution is 100～500mg:10ml.
3. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：In described step (3), gold nanorods solution with the volume ratio of polymer solution is
1:5～10.
4. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：In described step (4), pyrrole monomer with the volume ratio of gold nanorods solution is
0.025～0.1:1.
5. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：In described step (4), the temperature of low temperature environment is 1～5 DEG C.
6. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：The suspension liquid of gold nanorods/polypyrrole nanoparticle in described step (5)
Amass as 1～5mL, deionized water and ethanol volume ratio 5～8:1, the cumulative volume after suspension dilution is 5～30mL；Add twice
Ammonia spirit with dilution after suspension volume ratio be respectively 0.2～2:5～30.
7. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：In described step (5), temperature is increased to 50～80 DEG C.
8. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：In described step (6), the concentration of chitosan solution and sodium alginate soln is 5
～20mg/mL.
9. a kind of multi-functional being combined of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is received
Rice diagnosis and treatment agent preparation method it is characterised in that：N-Hydroxysuccinimide, N- (3- dimethylamino third is added in described step (7)
Base)-N'- ethyl-carbodiimide hydrochloride and Folic Acid be respectively 0.1～0.5g, 0.1～0.5g and 0.05～0.5g.
10. a kind of folacin coupled gold nanorods/polypyrrole/ferroso-ferric oxide according to claim 1 is multi-functional compound
Nanometer diagnosis and treatment agent preparation method it is characterised in that：In described step (8), triethylamine and the volume ratio of deionized water are 1～5:3
～30；The scope adjusting pH is 7.0～9.0.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106963744B (en) * | 2017-02-21 | 2020-05-05 | 上海工程技术大学 | Coupled folic acid targeted nano composite particle and preparation method and application thereof |
| CN108743980B (en) * | 2018-07-06 | 2021-05-18 | 重庆医科大学 | Folic acid targeted visual photothermal-chemotherapy therapeutic agent and preparation method thereof |
| CN108815525B (en) * | 2018-07-06 | 2021-04-13 | 重庆医科大学 | Multifunctional polypyrrole-coated drug-loaded mesoporous titanium dioxide nanoparticle and preparation method thereof |
| CN109289050B (en) * | 2018-11-14 | 2021-03-16 | 中国科学院上海硅酸盐研究所 | Ferroferric oxide/polypyrrole/glucose oxidase composite multifunctional nano diagnosis and treatment agent and preparation method and application thereof |
| CN111283214B (en) * | 2020-02-26 | 2022-08-05 | 西安工业大学 | Pure silver nanorod with hexagonal cross section and preparation method thereof |
| CN115737588B (en) * | 2022-10-14 | 2024-02-20 | 浙江大学 | Platelet membrane coated gold/ferric hydroxide nanocomposite and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008048074A1 (en) * | 2006-10-20 | 2008-04-24 | Seoul National University Industry Foundation | Use of core-shell gold nanoparticle which contains magnetic nanoparticles for mri t2 contrast agent, cancer diagnostic and therapy |
| CN102464885A (en) * | 2010-11-11 | 2012-05-23 | 西北师范大学 | Method for preparing gold-polypyrrole/prussian blue core-shell multi-nanocomposite at one step |
-
2014
- 2014-11-07 CN CN201410623958.6A patent/CN104436193B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008048074A1 (en) * | 2006-10-20 | 2008-04-24 | Seoul National University Industry Foundation | Use of core-shell gold nanoparticle which contains magnetic nanoparticles for mri t2 contrast agent, cancer diagnostic and therapy |
| CN102464885A (en) * | 2010-11-11 | 2012-05-23 | 西北师范大学 | Method for preparing gold-polypyrrole/prussian blue core-shell multi-nanocomposite at one step |
Non-Patent Citations (2)
| Title |
|---|
| Multifunctional Polypyrrole@Fe 3 O 4 Nanoparticles for Dual-Modal Imaging and In Vivo Photothermal Cancer Therapy;Qiwei Tian,et al;《small》;20131127;第10卷(第6期);第1163页右栏第24行—第1064页左栏第18行，第1166页右栏第13行—第1067页左栏第10行 * |
| Polypyrrole-Coated Chainlike Gold Nanoparticle Architectures with the 808 nm Photothermal Transduction Efficiency up to 70%;Min Lin,et al;《ACS Appl. Mater》;20140324;第6卷;第5866页右栏"CONCLUSIONS"部分 * |
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