CN104434983A - Extraction method of asterina pectinifera extract and application of extract in preparing anti-tumor medicines - Google Patents
Extraction method of asterina pectinifera extract and application of extract in preparing anti-tumor medicines Download PDFInfo
- Publication number
- CN104434983A CN104434983A CN201410752544.3A CN201410752544A CN104434983A CN 104434983 A CN104434983 A CN 104434983A CN 201410752544 A CN201410752544 A CN 201410752544A CN 104434983 A CN104434983 A CN 104434983A
- Authority
- CN
- China
- Prior art keywords
- extract
- mukller
- ethanol
- pectinifera
- tro sehel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/616—Echinodermata, e.g. starfish, sea cucumbers or sea urchins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of medicines and relates to an application of an asterina pectinifera extract in preparation of anti-tumor medicines and health foods. The extract is a dried asterina pectinifera whole body and is obtained by 75% ethanol extraction and elution and purification by macroporous resin. The extract obtained by the invention has relatively strong antitumor activity in vitro and can be used for preparing anti-tumor medicines and health foods with antitumor activity, such as anti-colon cancer medicines and the like.
Description
Technical field:
The present invention relates to medical art, be specifically related to the method for extraction and purification of Potiria pectinifera (Mukller et Tro Sehel) anti-tumor active ingredient and the application in antitumor drug preparation thereof.
Background technology:
Asterias amurensis Lutken is that coastal aquaculture brings huge economic loss, and the appetite of Asterias amurensis Lutken is surprising, and an Asterias amurensis Lutken can eat up tens scallops for 1 day, causes huge economic loss to shellfishery.In order to reduce the economic loss and harm that Asterias amurensis Lutken brings, administer in the urgent need to the disaster brought Asterias amurensis Lutken, especially to two Common Species, asterias rollestoni and the Potiria pectinifera (Mukller et Tro Sehel)s of in above-mentioned Asterias amurensis Lutken, Qingdao sea area being caused to serious harm.In recent years, the medical value of Asterias amurensis Lutken is progressively taken seriously, and records according to Chinese Sea medicine dictionary, and the Potiria pectinifera (Mukller et Tro Sehel) itself in the middle of these two kinds has very high medical value.The drying entirety of Potiria pectinifera (Mukller et Tro Sehel) can be used as medicine, and has effect of kidney invigorating and YANG supporting, wind-damp dispelling, pain relieving, cures mainly the symptoms such as sexual impotence, lumbago due to pathogenic wind-dampness, stomachache.
Not to the preparation method of Potiria pectinifera (Mukller et Tro Sehel) extract and the relevant report of its anti-tumor activity in prior art.
Summary of the invention:
The object of this invention is to provide a kind of extraction and process for purification of Potiria pectinifera (Mukller et Tro Sehel) extract, described Potiria pectinifera (Mukller et Tro Sehel) extract contains anti-tumor active ingredient, can be advantageously applied to and prepare antitumor drug.
Potiria pectinifera (Mukller et Tro Sehel) extract of the present invention obtains by following technical solution:
(1) Potiria pectinifera (Mukller et Tro Sehel) is dry all, extracts with 75% ethanol;
(2) extracting solution is separated through macroporous adsorbent resin after concentrated, first carries out eluting with pure water and 10% ethanol, then carries out eluting with 20%-60% ethanol, obtain eluent;
Wherein, the extraction time of step (1) is 2-3 time, and extraction time is 1-3 hour.
Wherein, the macroporous resin described in step (2) is selected from D101.
The separating obtained fraction of 40% to 60% ethanol macroporous adsorbent resin in step (2), shows to have the effect well suppressing human colon cancer cell HCT116 propagation through the test of body outer cell proliferation Inhibition test.
Potiria pectinifera (Mukller et Tro Sehel) extract of the present invention can form compositions to prepare antitumor drug and food and health product separately or with other pharmaceutically acceptable compositions.
Described antitumor drug dosage form is capsule, tablet, oral formulations, microcapsule formulation, injection, suppository, spray or ointment.
Accompanying drawing illustrates:
Fig. 1 is that Potiria pectinifera (Mukller et Tro Sehel) extract suppresses human colon carcinoma HCT116 cell-proliferation activity result.
HX-4: Potiria pectinifera (Mukller et Tro Sehel) 75% ethanol extraction macroporous adsorbent resin 40% ethanol elution fraction; HX-6: Potiria pectinifera (Mukller et Tro Sehel) 75% ethanol extraction macroporous adsorbent resin 60% ethanol elution fraction.
Detailed description of the invention: the present invention will be further described below in conjunction with example, but instantiation does not do any restriction to the present invention
Example 1: the preparation of Potiria pectinifera (Mukller et Tro Sehel) extract
(1) dry all 5 kilograms of Potiria pectinifera (Mukller et Tro Sehel) (Asterina pectinifera), carries out extraction 3 times with 3 times amount 75% ethanol, each 3 hours.Merge extractive liquid.
(2) extracting solution is after concentrated after drying, and gained extractum water dissolution, is separated through D101 type macroporous adsorbent resin, first carries out eluting with pure water and 10% ethanol, then carries out eluting with 20% to 60% ethanol, collects 40% to 60% ethanol elution.
Example 2: Potiria pectinifera (Mukller et Tro Sehel) extract suppresses human colon carcinoma HCT116 cell to increase active testing
(1) cell culture
Human colon cancer cell HCT116 is from Human Sciences Research Resource Bank (Japanese mankind NSFC, Tokyo, Japan) obtain, be incubated in the RPMI RPMI-1640 containing 10% hot deactivation calf serum, 37 DEG C, 5% CO
2, cultivate under saturated humidity condition.Choose exponential phase cell to test.
(2) body outer cell proliferation Inhibition test
MTT method (MTT) detects cell growth status, and PC3 cell is planted in 96 well culture plates respectively, and each porocyte number is 5 × 10
3, be incubated in the RPMI RPMI-1640 containing 10% hot deactivation calf serum, at 37 DEG C, 5% CO
224 h are cultivated under condition.After cultivating 24 h, rinse cell with PBS, then add new culture fluid and the testing sample of series concentration respectively.Experimental group establishes 6 drug level groups, and the final concentration often organizing medicine is respectively 5,10,20,50,100,200 μ g/ml, and matched group adds the dimethyl sulfoxide (DMSO) of same concentration, cultivates 24 h.Cultivation terminates the front 4 every holes of h and adds 20 μ L MTT solution (5mg/ml), centrifugally abandons supernatant, and add the abundant dissolving crystallized thing of 200 μ L DMSO, microplate reader surveys 570 nm place absorbances.Because absorbance is directly proportional to viable count in culture fluid, so cell survival percentage rate can be calculated by absorbance.As shown in Figure 1, show through gradient elution fraction tumor growth inhibiting activity result, Asterias amurensis Lutken 75% ethanol extraction is separated through macroporous adsorbent resin acquired results, and 40% to 60% eluting fraction has very strong vitro inhibition human colon carcinoma HCT116 cell-proliferation activity.
Claims (6)
1. a Potiria pectinifera (Mukller et Tro Sehel) extract, is characterized in that, preparation process is as follows:
(1) Potiria pectinifera (Mukller et Tro Sehel) is dry all, extracts with 75% ethanol;
(2) extracting solution is separated through macroporous adsorbent resin after concentrated, carries out eluting, obtain eluent with 20%, 40%, 60% ethanol;
(3) collect the separating obtained fraction of 40% to 60% ethanol macroporous adsorbent resin, after concentrate drying, obtain Potiria pectinifera (Mukller et Tro Sehel) targeted activity extract.
2. Potiria pectinifera (Mukller et Tro Sehel) extract according to claim 1, is characterized in that, in described step (1), organic solvent is 75% ethanol.
3. Potiria pectinifera (Mukller et Tro Sehel) extract according to claim 1, is characterized in that, in described step (2), gradient elution concentration is the ethanol of 40% to 60% percentage.
4. Potiria pectinifera (Mukller et Tro Sehel) extract according to claim 3, is characterized in that collection 40% to 60% ethanol elution.
5. Potiria pectinifera (Mukller et Tro Sehel) extract is preparing the application in antitumor drug according to claim 1.
6. according to claim 5, it is characterized in that described antitumor drug is drugs against colon cancer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410752544.3A CN104434983A (en) | 2014-12-10 | 2014-12-10 | Extraction method of asterina pectinifera extract and application of extract in preparing anti-tumor medicines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410752544.3A CN104434983A (en) | 2014-12-10 | 2014-12-10 | Extraction method of asterina pectinifera extract and application of extract in preparing anti-tumor medicines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104434983A true CN104434983A (en) | 2015-03-25 |
Family
ID=52882245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410752544.3A Pending CN104434983A (en) | 2014-12-10 | 2014-12-10 | Extraction method of asterina pectinifera extract and application of extract in preparing anti-tumor medicines |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104434983A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1545927A (en) * | 2003-12-06 | 2004-11-17 | 许淑芬 | Preparation method of raw material for producing health food and pharmaceutical |
| KR20060088911A (en) * | 2005-02-02 | 2006-08-07 | 김옥자 | A process for preparation of ethanol-extracts of asterina petinifera |
| CN1840539A (en) * | 2005-03-29 | 2006-10-04 | 郭承华 | Salangane saponin and preparation method and use thereof |
| KR20090025422A (en) * | 2007-09-06 | 2009-03-11 | 조월순 | Effect of antioxdant, wrinkles protection, antibacterial, anti-inflammatory and whitening from asterina pectinifera extract |
| KR20090045627A (en) * | 2007-11-02 | 2009-05-08 | 부경대학교 산학협력단 | Polysaccharides extracted from asterina pectinifera which have an detoxication effect on a living body, and the preparation method thereof |
| KR20110044485A (en) * | 2009-10-23 | 2011-04-29 | (주)엔엘피 | Ffect of whitening and uv protection from water soluble extract of asterina pectinifera |
-
2014
- 2014-12-10 CN CN201410752544.3A patent/CN104434983A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1545927A (en) * | 2003-12-06 | 2004-11-17 | 许淑芬 | Preparation method of raw material for producing health food and pharmaceutical |
| KR20060088911A (en) * | 2005-02-02 | 2006-08-07 | 김옥자 | A process for preparation of ethanol-extracts of asterina petinifera |
| CN1840539A (en) * | 2005-03-29 | 2006-10-04 | 郭承华 | Salangane saponin and preparation method and use thereof |
| KR20090025422A (en) * | 2007-09-06 | 2009-03-11 | 조월순 | Effect of antioxdant, wrinkles protection, antibacterial, anti-inflammatory and whitening from asterina pectinifera extract |
| KR20090045627A (en) * | 2007-11-02 | 2009-05-08 | 부경대학교 산학협력단 | Polysaccharides extracted from asterina pectinifera which have an detoxication effect on a living body, and the preparation method thereof |
| KR20110044485A (en) * | 2009-10-23 | 2011-04-29 | (주)엔엘피 | Ffect of whitening and uv protection from water soluble extract of asterina pectinifera |
Non-Patent Citations (4)
| Title |
|---|
| ZHANQIANG LI: "《Three new steroid glycosides from the starfish asterina pectinifera》", 《NATURAL PRODUCT RESEARCH》 * |
| 左海娇: "《海燕内脏脑苷脂的分离纯化及抗肿瘤活性研究》", 《大连海洋大学硕士学位论文》 * |
| 郭承华: "《海燕皂苷及其它活性物质的制备及其生物功能研究》", 《中国海洋大学 研究生学位论文》 * |
| 郭承华等: "《黄海产海燕（Asterina pectinifera）海星皂苷的制备 》", 《海洋通报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hu et al. | The research progresses and future prospects of Tetrastigma hemsleyanum Diels et Gilg: A valuable Chinese herbal medicine | |
| CN101181317B (en) | Purslane extract as well as preparation method and application thereof | |
| CN104825500B (en) | Ganoderma leucocontextum extract, extraction method and application thereof | |
| Liu et al. | An in vivo and in vitro assessment of the anti-inflammatory, antinociceptive, and immunomodulatory activities of Clematis terniflora DC. extract, participation of aurantiamide acetate | |
| CN103272007A (en) | Paeonia plant extract and preparation method and applications thereof | |
| CN103316096A (en) | General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof | |
| Okonogi et al. | Anti-inflammatory effects of compounds from Polygonum odoratum | |
| CN101824014B (en) | Compounds with anti-tumor activity in chloranthus japonicus and purpose thereof | |
| CN104382968B (en) | Extraction method of andrographolide, andrographolide pharmaceutical composition and application | |
| CN104274502B (en) | A kind of extracting method of the efficient antiinflammatory action compound of euscaphis konishii, product | |
| Zhang et al. | Effects of space flight on the chemical constituents and anti-inflammatory activity of licorice (Glycyrrhiza uralensis Fisch) | |
| CN106083556B (en) | Azulene structural compounds and its extraction separation method in purslane | |
| CN102659746A (en) | Ailanthus flower extract as well as preparation method and application of active ingredients brevifolin of ailanthus flower extract | |
| CN102898322B (en) | Compound and preparation method and application thereof | |
| CN104434983A (en) | Extraction method of asterina pectinifera extract and application of extract in preparing anti-tumor medicines | |
| CN111150754B (en) | Application of chestnut flower extract in preparation of food or anti-inflammatory drugs | |
| CN107308269A (en) | A kind of preparation method of the luxuriant boisiana extract of suede and its application of extract | |
| CN102988525A (en) | Preparation method for total lignans in hawthorn seeds, and novel application | |
| CN103420971B (en) | Antitumor compounds derived from G. nujiangnsis, and preparation method and application thereof | |
| CN102000136B (en) | Method for extracting components having antibacterial activity from fruit of pedunculate herpetospermum | |
| CN103520195A (en) | Application of clitocine in preparation of sensitizer | |
| CN109718268A (en) | The preparation method of Ormosia hosiei seed extract and its application in anti-inflammatory drug | |
| CN104147565B (en) | Extraction method for mioga ginger antineoplastic active component | |
| CN108295052A (en) | The application of I. henry Diels glycosides ether and composition in urarthritis | |
| US20210290712A1 (en) | Erodium crassifolium l'her plant extracts and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150325 |