CN104431987A - Method for extracting lipid-soluble antioxidant from aronia fruits - Google Patents

Method for extracting lipid-soluble antioxidant from aronia fruits Download PDF

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Publication number
CN104431987A
CN104431987A CN201410638158.1A CN201410638158A CN104431987A CN 104431987 A CN104431987 A CN 104431987A CN 201410638158 A CN201410638158 A CN 201410638158A CN 104431987 A CN104431987 A CN 104431987A
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China
Prior art keywords
extract
fruit
wild cherry
certain kind
berries
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Pending
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CN201410638158.1A
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Chinese (zh)
Inventor
侯相林
史俊燕
邓天昇
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Shanxi Institute of Coal Chemistry of CAS
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Shanxi Institute of Coal Chemistry of CAS
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Priority to CN201410638158.1A priority Critical patent/CN104431987A/en
Publication of CN104431987A publication Critical patent/CN104431987A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3472—Compounds of undetermined constitution obtained from animals or plants

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Fats And Perfumes (AREA)

Abstract

The invention discloses a method for extracting a lipid-soluble antioxidant from aronia fruits. The method comprises the following steps: selecting unrotten intact aronia fruits, cleaning and removing impurities; crushing the aronia fruits in a stirrer; adding the crushed fruits to a weak polar solvent, extracting, filtering, and repeatedly extracting a filter cake until an extracting liquid is colorless or light in color; and merging the extracting liquid, concentrating and drying to obtain an extract. The method has the advantages of being simple in process and easy to industrialize.

Description

A kind of method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit
Technical field
The invention belongs to a kind of method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit.
Background technology
The wild cherry certain kind of berries is rosaceous a kind of machaka, originates in eastern North America and to wet the woods and marsh, be integrate edible, medicinal, the rare tree of gardens and ecological equivalence value, its ripe berry fruit juice is slightly put sweet (moderate sugar content), micro-acid.Its fruit can be used for the Food & Drink such as processing fruit juice, fruit wine, jam, can, preserved fruit, and main sales feature is its antioxygenic property.Wild cherry certain kind of berries fruit and extract thereof have special curative effect to the cardiovascular and cerebrovascular disease such as heart disease, hypertension, and the researcher of Illinois state universities’ function food item has shown that wild cherry certain kind of berries fruit contains Therapeutic cancer and cardiopathic conclusion.The wild cherry certain kind of berries is also containing the composition useful to human body: vitamin B6, B12, E, C, folic acid, quininic acid, Polyphenol Acids, tannin, catechin, Mongolian oak flavine, phytomelin, aurantiamarin, resveratrol and anthocyanidin.There is the aromatic structures such as phenyl ring in these active skull cap components, is potential natural resource.
Daily life produce in the antioxidant used can be divided into synthetized oxidation preventive agent and natural, synthetized oxidation preventive agent mainly contains Butylated Hydroxytoluene (BHT), tert-butyl hydroxy returns fragrant ether (BHA), tertiary butylated hydroquinone (TBHQ) and n-propyl gallate (PG) etc.; Natural is mainly derived from certain plants, is obtained by solvent extraction, expensive, and thus synthetized oxidation preventive agent captures most market always.But toxicology test shows, a lot of natural not only toxicity is very little, but also has certain health care, and thus people more and more welcome natural.But naturally occurring antioxidant part in plant body exists with the form of glucosides, this part material better water-soluble, but fat-soluble poor, directly can not be used in plant wet goods low pole and eat in article; Another part is then exist with free non-glycoside forms, fat-soluble good, can be advantageously used in edible oil materials.The method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit of the present invention finds no similar prior art through retrieval.
Summary of the invention
The object of this invention is to provide a kind of method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit.
The present invention adopts following technical scheme:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) joined in weak polar solvent by the fruit of fragmentation and extract, filter, filter cake repeats to extract, until extracting liquid colourless or very slight color, extract merges, and concentrate drying obtains extract.
As described in step (2), weak polar solvent used can be n-hexane, acetone, ethyl acetate, cyclohexane, benzinum (30 DEG C-60 DEG C, 60 DEG C-90 DEG C, 90 DEG C-120 DEG C of three kinds of boiling ranges) etc.
As described in step (2), the mass ratio of weak polar solvent volume and the wild cherry certain kind of berries is 10ml:0.5-10g, and Extracting temperature is 20 DEG C-60 DEG C, and extraction time is 40min-7h.
Advantage of the present invention:
Process of the present invention is simply easy to industrialization, and extract shows good non-oxidizability in grease.
Detailed description of the invention
Embodiment 1:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation is joined 10ml ethyl acetate, at 20 DEG C, extract 40min, filter, filter cake repeats to be extracted into colourless or very slight color, by filtrate reduced in volume, and dry, obtain extract 0.19g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.
The peroxide value of grease is lower, illustrates that the effect of antioxidant is better.As can be seen from the above table, along with the prolongation of oxidization time by force, the peroxide value of sample is all in increase tendency, contrast with blank sample, with the addition of the sample of wild cherry certain kind of berries extract to grease Wheat Protein, and along with the increase of extract concentrations, the peroxide value of identical oxidization time grease is less, illustrates that wild cherry certain kind of berries extract has strong antioxidant action to grease.
Embodiment 2:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation is joined 20ml acetone, at 25 DEG C, extract 1h, filter, filter cake repeats to be extracted into colourless or very slight color, and by filtrate reduced in volume, and drying obtains extract 0.2g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.
Embodiment 3:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation is joined 60ml cyclohexane, at 30 DEG C, extract 2h, filter, filter cake repeats to be extracted into colourless or very slight color, and by filtrate reduced in volume, and drying obtains extract 0.21g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.
Embodiment four:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation is joined 80ml n-hexane, at 40 DEG C, extract 4h, filter, filter cake repeats to be extracted into colourless or very slight color, and by filtrate reduced in volume, and drying obtains extract 0.19g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.
Embodiment five:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation being joined 100ml boiling range is in 30 DEG C of-60 DEG C of benzinums, at 45 DEG C, extract 5h, and filter, filter cake repeats to be extracted into colourless or very slight color, and by filtrate reduced in volume, and drying obtains extract 0.22g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.
Embodiment six:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation being joined 160ml boiling range is in 60 DEG C of-90 DEG C of benzinums, at 50 DEG C, extract 6h, and filter, filter cake repeats to be extracted into colourless or very slight color, and by filtrate reduced in volume, and drying obtains extract 0.21g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.
Embodiment seven:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer.
(2) the fruit 10g of fragmentation being joined 200ml boiling range is in 90 DEG C of-120 DEG C of benzinums, at 60 DEG C, extract 7h, and filter, filter cake repeats to be extracted into colourless or very slight color, and by filtrate reduced in volume, and drying obtains extract 0.21g.
(3) 10mg and 40mg extract is joined respectively in the peony seed oil of 10g laboratory brand-new, the peony seed oil of the 10g laboratory brand-new of not adding extract is set to blank, be placed in 60 DEG C of water proof constant incubators simultaneously, every day timing agitation once, exchange the position of sample in constant incubator, and get a sample determination peroxide value.The mensuration of peroxide value is pressed GB/T5009.37-2003 method and is measured.

Claims (4)

1. from wild cherry certain kind of berries fruit, extract a method for fat-soluble antioxidant, it is characterized in that comprising the steps:
(1) fruit pretreatment: select and clean impurity elimination without the complete wild cherry certain kind of berries fruit that rots, broken in mixer;
(2) joined in weak polar solvent by the fruit of fragmentation and extract, filter, filter cake repeats to extract, until extracting liquid colourless or very slight color, extract merges, and concentrate drying obtains extract.
2. a kind of method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit as claimed in claim 1, is characterized in that described in step (2), weak polar solvent be n-hexane, acetone, ethyl acetate, cyclohexane or benzinum.
3. a kind of method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit as claimed in claim 2, is characterized in that described benzinum is the benzinum of 30 DEG C-60 DEG C, 60 DEG C-90 DEG C or 90 DEG C-120 DEG C of three kinds of boiling ranges.
4. a kind of method extracting fat-soluble antioxidant from wild cherry certain kind of berries fruit as claimed in claim 1, the mass ratio that it is characterized in that the described weak polar solvent volume of step (2) and the wild cherry certain kind of berries is 10ml:0.5-10g, Extracting temperature is 20 DEG C-60 DEG C, and extraction time is 40min-7h.
CN201410638158.1A 2014-11-13 2014-11-13 Method for extracting lipid-soluble antioxidant from aronia fruits Pending CN104431987A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2171111C1 (en) * 2000-05-05 2001-07-27 Научно-исследовательский институт биомедицинской химии РАМН Aronia leaves extract showing biological activity and method of its preparing
CN101591325A (en) * 2008-05-26 2009-12-02 河南大学 A kind of method of extracting catechin
JP2010105937A (en) * 2008-10-29 2010-05-13 Pola Chem Ind Inc Glutathione production promoting composition
CN102028060A (en) * 2010-11-02 2011-04-27 浙江大学 Method for preparing extract of oil soluble tea

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2171111C1 (en) * 2000-05-05 2001-07-27 Научно-исследовательский институт биомедицинской химии РАМН Aronia leaves extract showing biological activity and method of its preparing
CN101591325A (en) * 2008-05-26 2009-12-02 河南大学 A kind of method of extracting catechin
JP2010105937A (en) * 2008-10-29 2010-05-13 Pola Chem Ind Inc Glutathione production promoting composition
CN102028060A (en) * 2010-11-02 2011-04-27 浙江大学 Method for preparing extract of oil soluble tea

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
D. POTTER: "Phylogeny and classification of Rosaceae", 《PLANT SYSTEMATICS AND EVOLUTION》 *
刘全坤: "《要健康就要这样吃》", 31 January 2011, 军事医学科学出版社 *
李莉，等: "新疆黑加仑提取物体外抗氧化活性的研究", 《食品与发酵工业》 *

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Application publication date: 20150325