CN104407123A - Biosensor and preparation method thereof - Google Patents
Biosensor and preparation method thereof Download PDFInfo
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- CN104407123A CN104407123A CN201410345231.6A CN201410345231A CN104407123A CN 104407123 A CN104407123 A CN 104407123A CN 201410345231 A CN201410345231 A CN 201410345231A CN 104407123 A CN104407123 A CN 104407123A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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Abstract
The invention discloses a biosensor and its preparation method. The preparation method comprises the following steps: 1) a PCB, a raw material plate is used as a substrate panel, and an insulated substrate and a conductive part of the biosensor are manufactured by a PCB manufacturing technology, wherein the conductive part contains various wires and electrodes; 2) different electrodes of the conductive part are coated with different electrode materials by an electroplating method or a chemical method to prepare various special electrodes required by the biosensor, and baseboard of the biosensor is obtained; and 3) the baseboard prepared in the step 2) is used to prepare the biosensor provided by the invention. In comparison with the prior art, the baseboard of the biosensor provided by the invention is prepared by the PCB technology, and the biosensor provided by the invention has advantages of simple technology and low cost. Thus, high performance and low cost can be achieved. The biosensor is especially suitable for instant detection of various biological signals such as various blood parameters (blood clotting,blood sugar, cardiac troponin and the like), contagious virus (tuberculosis, hepatitis B, HIV, etc.) and the like.
Description
Technical field
The invention belongs to field of biosensors, more particularly, the present invention relates to biology sensor of the high-performance and low-cost that one utilizes printed circuit board (PCB) (PCB) technique to make and preparation method thereof.
Background technology
Along with the development of various technology, biology sensor and corresponding detecting instrument can become more and more less, thus make the instant detection (POCT) in clinical medicine become may and developed rapidly.
The major advantage of POCT in minutes can obtain assay, therefore medical worker can carry out direct-detection to patient at one's side and carry out the Diagnosis and Treat of disease according to testing result, and do not need to be delivered to by sample centralab as traditional detection method, then wait for that result is to carry out follow-up work; For the medical field of " time is life ", this is obviously very useful.Such as: when emergency department have received the uncomfortable patient in chest, if cardiac muscle troponin I (cTnI) concentration in patient blood can be detected fast, whether doctor just quick diagnosis patient can suffer from myocardial infarction, thus makes corresponding process to patient; And in large-scale Surgical Operating Room, doctor also wishes some the crucial blood parameters knowing patient at any time, to determine to transfuse blood the need of to patient.If above situation traditional detection method, the blood sample of patient is all needed to deliver to centralab, again result is sent back to corresponding section office after testing, because whole flow process involves several step formality, so from drawing blood to result, at the soonest a hours, this has delayed the quality time of a lot of curing patient undoubtedly, even likely therefore jeopardizes the life of patient.
On the other hand, the client need such as venae profunda conducted and cardiac valve replacement long-term taking anticoagulant, in case tampon is formed, but anticoagulant can not be taken too much again, otherwise will have the danger losing hemostatic function.Therefore, client need periodic detection coagulation indexes is as prothrombin time (PT).In recent years, the self-inspection of PT is risen gradually at Main Developed Countries, but does not also carry out in developing country, and chief reason is exactly that self-inspection instrument and the price trying bar are too high.
The self-inspection of diabetes patient is the POCT the most successful the most typical example.This is because other physiological parameter relatively, blood sugar concentration is higher, can produce stronger reaction signal in blood glucose sensor, such as can produce the redox current of microampere order in Electrochemical blood glucose sensor.Therefore, the production technology of blood glucose sensor is fairly simple comparatively speaking, and such as, its electrode screen printing technique makes; Because this technique can quickly be produced in enormous quantities, so cost can be very low, and use also very simple.This makes most of diabetes patient to carry out self-inspection, and also affords economically.
But by contrast, some physiological parameter is as cTnI and PT, and the concentration of its tested substance is very low, and the signal that corresponding sensor produces is very weak, thus require very high to the electrode of sensor, so that the screen printing technique of low cost cannot meet testing requirement.In order to reach higher precision, the electrode of these sensors must adopt other production technology to make, such as: the sensor of Abbott Laboratories i-STAT series makes matrix with silicon wafer, metal material is coated, chemically unwanted partial corrosion is fallen again, remaining just defines electrode (US Patent5,837,454).The sensor accuracy prepared of said method is fine, but due to silicon wafer more expensive, add that the precious metal wasted in production run is as more in gold, platinum etc., so the overall cost of sensor is higher, sensor is also just difficult to promote the use of.
In view of this, necessaryly biology sensor of a kind of high-performance and low-cost and preparation method thereof is provided, to promote the clinical practice of POCT.
Summary of the invention
The object of the invention is to: a kind of high-performance and low-cost, the biology sensor being suitable for POCT and preparation method thereof are provided, to promote the clinical practice of POCT.
In order to realize foregoing invention object, the invention provides a kind of biology sensor, it comprises the base plate utilizing pcb board technique to make, base plate comprises dielectric substrate and is attached to the conductive component on dielectric substrate one or both sides, wherein, the material of dielectric substrate is identical with the dielectric substrate of PCB raw material plate, and conductive component comprises the common conductive parts identical with the conductive layer material of PCB raw material plate and on common conductive parts, covers the special electrode that Different electrodes material makes.
One as biology sensor of the present invention is improved, and described special electrode is included on common conductive parts and covers electrode that inert metal makes with electrochemical plating or chemical method, on common conductive parts, cover one or both in the electrode that silver/silver chloride makes with electrochemical plating or chemical method; Described inert metal is preferably gold or platinum.
One as biology sensor of the present invention is improved, and described common conductive parts comprise for the current-carrying plate of connecting sensor and analyser, wire and one or more for monitoring in electrode that sample adds.
One as biology sensor of the present invention is improved, and the base plate of described biology sensor also comprises the heating resistor of the various shapes being attached to dielectric substrate front or reverse side, and heating resistor is used for heating to sensor.
One as biology sensor of the present invention is improved, and the base plate of described biology sensor can have the collecting of various shape or load bearing component to be used for placing the various material such as reagent, antigen, antibody, magnetic bead needed for sensor, such as, put the guard ring of reagent; According to actual needs, the shape of these collectings or load bearing component can be the difformities such as nahlock, circle, multiple concentric circles, square, grid.
One as biology sensor of the present invention is improved, and described biology sensor is amperometric electrochemical sensor, voltage-type electrochemical sensor or impedance type biology sensor.
One as biology sensor of the present invention is improved, the base plate of described amperometric electrochemical sensor be provided with the current-carrying plate of connecting sensor and analyser, wire, for monitor electrode that sample adds, working electrode, to electrode, the guard ring putting reagent and heating resistor; Wherein, working electrode covers gold with electrochemical plating or chemical method on substrate copper or platinum is formed, and reacts again formed electrode on substrate copper with electrochemical plating or chemical method after covering silver/silver chloride or cover fine silver with electrochemical plating or chemical method on substrate copper with villaumite.
One as biology sensor of the present invention is improved, the base plate of described voltage-type electrochemical sensor be provided with the current-carrying plate of connecting sensor and analyser, wire, for monitor electrode that sample adds, working electrode, to the guard ring of electrode, contrast electrode, storing reagent and heating resistor; Wherein, working electrode, all with electrochemical plating or chemical method, silver/silver chloride is covered to electrode, contrast electrode on substrate copper or cover fine silver with electrochemical plating or chemical method on substrate copper after react with villaumite again and formed.
One as biology sensor of the present invention is improved, and the base plate of described impedance type biology sensor is provided with the current-carrying plate of connecting sensor and analyser, wire, heating resistor, two for applying the electrode of electric current and two for detecting the electrode of voltage; Wherein, on substrate copper, all cover silver/silver chloride with electrochemical plating or chemical method or react with villaumite again cover fine silver with electrochemical plating or chemical method on substrate copper after formed for applying the electrode of electric current and the electrode for detecting voltage, and two for the electrode that detects voltage two for apply electric current electrode between.
In order to realize foregoing invention object, present invention also offers a kind of preparation method of biology sensor, it comprises the following steps:
1) using PCB raw material plate as matrix sheet material, produced dielectric substrate and the conductive component of biology sensor by PCB manufacturing process, conductive component comprises various line and electrode;
2) on the Different electrodes of conductive component, cover Different electrodes material with electrochemical plating or chemical method, make the various special electrodes needed for biology sensor, obtain biology sensor base plate;
3) step 2 is utilized) obtained base plate prepares biology sensor.
One as the preparation method of biology sensor of the present invention is improved, and described PCB raw material plate comprises dielectric substrate and conductive layer.
One as the preparation method of biology sensor of the present invention is improved, and the dielectric substrate of described PCB raw material plate is the dielectric materials such as bakelite plate, papery resin plate, glass resin plate, stupalith plate; Conductive layer is generally Copper Foil, but also can be other conductive metal material as required, and such as, wherein one side can be resistance material, to make heating resistor.
One as the preparation method of biology sensor of the present invention is improved, described heating resistor can by corroding into very elongated conductive filament to make by the Copper Foil at the pcb board back side, because the cross-sectional area of conductive filament is very little relative to its length, therefore will have certain resistance and heating can be done; In addition, PCB raw material plate can also be made one side is general Copper Foil, and another side is common resistance material, then with similar chemical corrosion method, resistance material is made into suitable shape.
One as the preparation method of biology sensor of the present invention is improved, described conductive component be by the PCB chemical etching technology of standard by unwanted conductive material in PCB raw material plate conductive layer as Copper Foil remove after stay.
One as the preparation method of biology sensor of the present invention is improved, and the various special electrodes needed for described biology sensor comprise with inert metal as gold or the platinum electrode that is electrode material, with one or both in silver/silver chloride electrode that is electrode material.
One as the preparation method of biology sensor of the present invention is improved, and described is golden or platinum to be covered on the substrate copper of conductive component respective electrode with standard electric plating method or chemical method to obtain with gold or the platinum electrode that is electrode material; The described electrode that is electrode material with silver/silver chloride be silver/silver chloride covered conductive component respective electrode with standard electric plating method or chemical method substrate copper on obtain, or first fine silver electrochemical plating or chemical method are covered on the substrate copper of respective electrode, then make with villaumite and silver hair biochemical reaction generation silver chloride.
One as the preparation method of biology sensor of the present invention is improved, and electrode and the final obtained various special electrodes needed for biology sensor of described conductive component can be that various shape is as nahlock, circle, multiple concentric circles, square, grid etc. all respectively.
Compared with prior art, the base plate of biology sensor of the present invention utilizes PCB technology to be made, there is the advantage that technique is simple, cheap, therefore can accomplish high-performance, low cost, be particularly suitable for the instant detection of various bio signal as various blood parameters (blood coagulation, blood sugar, cardiac troponin etc.), virus spread (tuberculosis, hepatitis B, HIV etc.) etc.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, biology sensor of the present invention, preparation method and beneficial effect thereof are further elaborated.
The interlayer structure schematic diagram of the PCB raw material plate that Fig. 1 uses for the embodiment of the present invention 1.
Fig. 2 is the Facad structure schematic diagram of the base plate of the amperometric electrochemical sensor that the embodiment of the present invention 1 obtains.
Fig. 3 is the inverse layer structure schematic diagram of the base plate of the amperometric electrochemical sensor that the embodiment of the present invention 1 obtains.
Fig. 4 is the Facad structure schematic diagram of the base plate of the electric potential type electrochemical sensor that the embodiment of the present invention 2 obtains.
Fig. 5 is the Facad structure schematic diagram of the base plate of the impedance type biology sensor that the embodiment of the present invention 3 obtains.
Embodiment
In order to make goal of the invention of the present invention, technical scheme and Advantageous Effects more clear, below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but, should be understood that, embodiments of the invention are only used to explain the present invention, be not intended to limit the present invention, and embodiments of the invention are not limited to the embodiment provided in instructions.
It should be noted that, following examples only describe the bottom parts (comprising dielectric substrate and conductive component) of various biology sensor; But in fact, a complete biology sensor examination bar at least also needs to arrange a cover plate and a sealed film, cover plate should have some be convenient to sample flows to reaction zone flow passage from porch, base plate and cover plate then bond together by sealed film, flow passage and reaction zone are sealed simultaneously, spill to prevent sample.Due to the parts that above-mentioned two parts are all non-normal opens, not emphasis of the present invention, therefore repeats no more in embodiment.
Embodiment 1
Refer to Fig. 1 to Fig. 3, the step utilizing the preparation method of biology sensor of the present invention to make amperometric electrochemical sensor base plate is:
1) select general PCB raw material plate as matrix sheet material, the dielectric substrate 12 of PCB raw material plate can be bakelite plate, papery resin plate, glass resin plate, stupalith plate etc., and conductive layer 13 is generally Copper Foil;
2) by the PCB chemical etching technology of standard, removed by unnecessary Copper Foil, remaining is required conductive component, comprises common conductive parts, the reaction electrode matrix for electrochemical reaction, the guard ring 19 putting reagent and heating resistor 20; Wherein, common conductive parts comprise for the current-carrying plate 14 of connecting sensor and analyser, wire 15 and for monitoring the electrode 16 that sample adds; Except two reaction electrodes 17,18, namely other conductive component is made by this step;
3) with standard electric plating method or chemical method, gold or platinum are covered on the substrate copper of working electrode 17, silver/silver chloride is covered on the substrate copper to electrode 18, complete the making of amperometric electrochemical sensor base plate; Certainly, also can be first fine silver is covered on substrate copper to the making of electrode 18, then produce chemical reaction with villaumite and silver and generate required silver chloride.
Utilize the amperometric electrochemical sensor base plate that above-mentioned steps is obtained, comprise dielectric substrate and be attached to the conductive component on dielectric substrate two sides respectively.Wherein, the conductive component in front comprise the current-carrying plate 14 of connecting sensor and analyser, wire 15, for monitor electrode 16 that sample adds, working electrode 17, to electrode 18 and the guard ring 19 putting reagent; The conductive component of reverse side only comprises heating resistor 20.Working electrode 17 on substrate copper, cover gold or platinum is formed; On substrate copper, cover silver/silver chloride to electrode 18 to be formed.
Needs according to different: reagent guard ring 19 can make different shapes as circular or square etc., and also can be placed on different places as in surrounding them or flow passage, can also be one or more.Different reagent is placed in reagent guard ring 19, namely can be used as the biology sensor measuring different biological parameter, such as, put factor I (thromboplastin) and fibrin ferment (thrombin) substrate, just can be used as prothrombin time (PT) sensor; Put suitable antigen-antibody and just can be used as immune-electrochemistry sensor, to measure various physiological marker thing as cTnI, TB, HIV etc.Mentioned reagent or antigen-antibody can be placed into reagent guard ring 19 li, such as contact printing, contactless spraying etc. by conventional method.
Heating resistor 20 can by making snakelike resistance wire to realize by Copper Foil, and its resistance value is adjusted by the width and length changing resistance wire; If necessary, the metal or alloy that resistivity can be used higher replaces copper, to obtain suitable resistance value.
Embodiment 2
Refer to Fig. 4, the step utilizing the preparation method of biology sensor of the present invention to make voltage-type electrochemical sensor base plate is:
1) select the universal PC B raw material plate identical with embodiment 1 as matrix sheet material;
2) by the PCB chemical etching technology of standard, unnecessary Copper Foil is removed, remaining is required conductive component, comprise common conductive parts, the reaction electrode matrix for electrochemical reaction, the guard ring 19 putting reagent and heating resistor (identical with embodiment 1, to scheme not show); Wherein, common conductive parts comprise for the current-carrying plate 14 of connecting sensor and analyser, wire 15 and for monitoring the electrode 34 that sample adds; Except three reaction electrodes 31,32,33, namely other conductive component is made by this step;
3) with standard electric plating method or chemical method, silver/silver chloride is covered on the substrate copper of three reaction electrodes 31,32,33, obtain electrode 31, working electrode 32 and contrast electrode 33; Certainly, also can be first fine silver electrochemical plating or chemical method are covered on substrate copper to the making of electrode 31, working electrode 32 and contrast electrode 33, then produce chemical reaction with villaumite and silver and generate required silver chloride;
4) on working electrode 32, different ion-selective membranes (ISE) is put, contrast electrode 33 adds last layer high salt concentration as potassium chloride (KCl), namely can be used as the sensor measuring different biological parameter, as various electrolytical ion concentrations such as the sodium in blood, potassium, calcium; Because the principle of work of above-mentioned electrode 32,33 is known by insider, therefore the present invention repeats no more.
Utilize the voltage-type electrochemical sensor base plate that above-mentioned steps is obtained, comprise dielectric substrate and be attached to the conductive component on dielectric substrate two sides respectively.Wherein, the conductive component in front comprise the current-carrying plate 14 of connecting sensor and analyser, wire 15, for monitoring electrode 34 that sample adds, to electrode 31, working electrode 32, contrast electrode 33 and the guard ring 19 putting reagent; The conductive component of reverse side only comprises heating resistor.On substrate copper, all cover silver/silver chloride to electrode 31, working electrode 32 and contrast electrode 33 to be formed.
Embodiment 3
Refer to Fig. 5, the step utilizing the preparation method of biology sensor of the present invention to make impedance type biology sensor base plate is:
1) select the universal PC B raw material plate identical with embodiment 1 as matrix sheet material;
2) by the PCB chemical etching technology of standard, unnecessary Copper Foil is removed, remaining is required conductive component, comprises common conductive parts, reaction electrode matrix and heating resistor (identical with embodiment 1, figure does not show) for electrochemical reaction; Wherein, common conductive parts comprise current-carrying plate 14 for connecting sensor and analyser and wire 15; Except four electrodes 41,42,43,44, namely other conductive component is made by this step;
3) with standard electric plating method or chemical method, silver/silver chloride is covered on the substrate copper of four electrodes 41,42,43,44, obtaining two for applying 41,44 and two, the electrode of electric current for detecting the electrode 42,43 of voltage, completing the making of impedance type biology sensor base plate; Certainly, the making of four electrodes 41,42,43,44 also can be first fine silver electrochemical plating or chemical method are covered on substrate copper, then produces chemical reaction with villaumite and silver and generate required silver chloride.
Utilize the impedance type biology sensor base plate that above-mentioned steps is obtained, comprise dielectric substrate and be attached to the conductive component on dielectric substrate two sides respectively.Wherein, the conductive component in front comprises the current-carrying plate 14 of connecting sensor and analyser, 15, two, wire for applying 41,44 and two, the electrode of electric current for detecting the electrode 42,43 of voltage; The conductive component of reverse side only comprises heating resistor.On substrate copper, all cover silver/silver chloride formed for applying the electrode 41,44 of electric current and the electrode 42,43 for detecting voltage, and two for the electrode 42,43 that detects voltage two for apply electric current electrode 41,44 between.
The preparation of biology sensor
When utilizing the obtained various base plates of embodiment 1-3 to prepare biology sensor, only with the PCB cutting technique of standard, the base plate processed need be cut into single examination bar, again according to actual needs, with various method as contact test print or contactless spray-on process by the file printings such as reagent, antigen, antibody or magnetic bead to examination bar on, then with double coated film, cover plate and base plate are sticked together.
Known by describing above, the biology sensor that the present invention utilizes PCB technology to make, there is the advantage that technique is simple, cheap, therefore can accomplish high-performance, low cost, be particularly suitable for the instant detection of various bio signal as various blood parameters (blood coagulation, blood sugar, cardiac troponin etc.), virus spread (tuberculosis, hepatitis B, HIV etc.) etc.
The announcement of book and instruction according to the above description, those skilled in the art in the invention can also carry out suitable change and amendment to above-mentioned embodiment.Therefore, the present invention is not limited to embodiment disclosed and described above, also should fall in the protection domain of claim of the present invention modifications and changes more of the present invention.In addition, although employ some specific terms in this instructions, these terms just for convenience of description, do not form any restriction to the present invention.
Claims (10)
1. a biology sensor, is characterized in that, described biology sensor comprises the base plate utilizing pcb board technique to make; Described base plate comprises dielectric substrate and is attached to the conductive component on dielectric substrate one or both sides, wherein, the material of dielectric substrate is identical with the dielectric substrate of PCB raw material plate, the special electrode that conductive component comprises the common conductive parts identical with the conductive layer material of PCB raw material plate and covers Different electrodes material and make on common conductive parts.
2. biology sensor according to claim 1, it is characterized in that, described special electrode is included on common conductive parts and covers electrode that inert metal makes with electrochemical plating or chemical method, on common conductive parts, cover one or both in the electrode that silver/silver chloride makes with electrochemical plating or chemical method; Described inert metal is preferably gold or platinum.
3. biology sensor according to claim 1, is characterized in that, described common conductive parts comprise current-carrying plate for connecting biology sensor and analyser, wire, one or more for monitoring in electrode that sample adds.
4. biology sensor according to claim 1, it is characterized in that, the base plate of described biology sensor being also optionally provided with the heating resistor for heating to sensor, being used for placing one in the collecting of sensor material requested or load bearing component, said sensor material requested includes but not limited to reagent, antigen, antibody, the magnetic bead needed for sensor herein.
5. biology sensor according to claim 1, is characterized in that, described biology sensor is amperometric electrochemical sensor, voltage-type electrochemical sensor or impedance type biology sensor.
6. biology sensor according to claim 5, is characterized in that,
The base plate of described amperometric electrochemical sensor be provided with the current-carrying plate of connecting sensor and analyser, wire, for monitor electrode that sample adds, working electrode, to electrode, the guard ring putting reagent and heating resistor; Wherein, working electrode covers gold with electrochemical plating or chemical method on substrate copper or platinum is formed, and reacts again formed electrode on substrate copper with electrochemical plating or chemical method after covering silver/silver chloride or cover fine silver with electrochemical plating or chemical method on substrate copper with villaumite;
The base plate of described voltage-type electrochemical sensor be provided with the current-carrying plate of connecting sensor and analyser, wire, for monitor electrode that sample adds, working electrode, to the guard ring of electrode, contrast electrode, storing reagent and heating resistor; Wherein, working electrode, all with electrochemical plating or chemical method, silver/silver chloride is covered to electrode, contrast electrode on substrate copper or cover fine silver with electrochemical plating or chemical method on substrate copper after react with villaumite again and formed;
The base plate of described impedance type biology sensor is provided with the current-carrying plate of connecting sensor and analyser, wire, heating resistor, two for applying the electrode of electric current and two for detecting the electrode of voltage; Wherein, on substrate copper, all cover silver/silver chloride with electrochemical plating or chemical method or react with villaumite again cover fine silver with electrochemical plating or chemical method on substrate copper after formed for applying the electrode of electric current and the electrode for detecting voltage, and two for the electrode that detects voltage two for apply electric current electrode between.
7. a preparation method for biology sensor, is characterized in that, comprises the following steps:
1) using PCB raw material plate as matrix sheet material, produced dielectric substrate and the conductive component of biology sensor by PCB manufacturing process, conductive component comprises various line and electrode;
2) on the Different electrodes of conductive component, cover Different electrodes material with electrochemical plating or chemical method, make the various special electrodes needed for biology sensor, obtain biology sensor base plate;
3) step 2 is utilized) obtained base plate prepares biology sensor.
8. the preparation method of biology sensor according to claim 7, is characterized in that, described PCB raw material plate comprises dielectric substrate and conductive layer; Wherein, the dielectric substrate of PCB raw material plate is selected from bakelite plate, papery resin plate, glass resin plate, stupalith plate, and conductive layer is Copper Foil or alloy material.
9. the preparation method of biology sensor according to claim 7, is characterized in that, described conductive component is stayed after removing by unwanted conductive material in PCB raw material plate conductive layer by the PCB chemical etching technology of standard.
10. the preparation method of biology sensor according to claim 7, is characterized in that, the various special electrodes needed for described biology sensor comprise with gold or the platinum electrode that is electrode material, with one or both in silver/silver chloride electrode that is electrode material; Wherein, be with standard electric plating method or chemical method golden or platinum covered on the substrate copper of conductive component respective electrode to obtain with gold or the platinum electrode that is electrode material; The electrode being electrode material with silver/silver chloride be silver/silver chloride covered conductive component respective electrode with standard electric plating method or chemical method substrate copper on obtain, or first fine silver electrochemical plating or chemical method are covered on the substrate copper of respective electrode, then make with villaumite and silver hair biochemical reaction generation silver chloride.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410345231.6A CN104407123A (en) | 2014-07-18 | 2014-07-18 | Biosensor and preparation method thereof |
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| CN201410345231.6A CN104407123A (en) | 2014-07-18 | 2014-07-18 | Biosensor and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105738615A (en) * | 2016-04-15 | 2016-07-06 | 张红 | Immunodetection device and method based on noble electrode steady state electrical state measurement |
| CN107687869A (en) * | 2016-08-05 | 2018-02-13 | 思科技术公司 | For the method and apparatus for the corrosive environment for monitoring electrical equipment |
| WO2018103116A1 (en) * | 2016-12-09 | 2018-06-14 | 广州好芝生物科技有限公司 | Prothrombin time detection method and device |
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| WO2001011080A1 (en) * | 1999-08-04 | 2001-02-15 | Andcare, Inc. | A printed circuit board, biosensor and method of using same |
| CN101275925A (en) * | 2007-03-28 | 2008-10-01 | 爱-森斯株式会社 | Electrochemical biosensor and biosensor measuring device |
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2014
- 2014-07-18 CN CN201410345231.6A patent/CN104407123A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001011080A1 (en) * | 1999-08-04 | 2001-02-15 | Andcare, Inc. | A printed circuit board, biosensor and method of using same |
| CN101275925A (en) * | 2007-03-28 | 2008-10-01 | 爱-森斯株式会社 | Electrochemical biosensor and biosensor measuring device |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105738615A (en) * | 2016-04-15 | 2016-07-06 | 张红 | Immunodetection device and method based on noble electrode steady state electrical state measurement |
| CN107687869A (en) * | 2016-08-05 | 2018-02-13 | 思科技术公司 | For the method and apparatus for the corrosive environment for monitoring electrical equipment |
| CN107687869B (en) * | 2016-08-05 | 2020-05-19 | 思科技术公司 | Method and device for monitoring the corrosive environment of an electrical apparatus |
| US12019010B2 (en) | 2016-08-05 | 2024-06-25 | Cisco Technology, Inc. | Method and apparatus for monitoring a corrosive environment for electrical equipment |
| WO2018103116A1 (en) * | 2016-12-09 | 2018-06-14 | 广州好芝生物科技有限公司 | Prothrombin time detection method and device |
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Application publication date: 20150311 |