CN104402833B - Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes - Google Patents

Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes Download PDF

Info

Publication number
CN104402833B
CN104402833B CN201410667916.2A CN201410667916A CN104402833B CN 104402833 B CN104402833 B CN 104402833B CN 201410667916 A CN201410667916 A CN 201410667916A CN 104402833 B CN104402833 B CN 104402833B
Authority
CN
China
Prior art keywords
compound
methyl
amino
alkyl
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201410667916.2A
Other languages
Chinese (zh)
Other versions
CN104402833A (en
Inventor
任青云
刘辛昌
唐昌华
张健存
张英俊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Haitong Hengxin International Finance Leasing Tianjin Co ltd
Original Assignee
Guangdong HEC Pharmaceutical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong HEC Pharmaceutical filed Critical Guangdong HEC Pharmaceutical
Priority to CN201410667916.2A priority Critical patent/CN104402833B/en
Publication of CN104402833A publication Critical patent/CN104402833A/en
Application granted granted Critical
Publication of CN104402833B publication Critical patent/CN104402833B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D241/24—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D241/26—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with nitrogen atoms directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D241/24—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D241/26—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with nitrogen atoms directly attached to ring carbon atoms
    • C07D241/28—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with nitrogen atoms directly attached to ring carbon atoms in which said hetero-bound carbon atoms have double bonds to oxygen, sulfur or nitrogen atoms

Abstract

The present invention relates to a kind of pyrazine compounds containing cyclobutane substituent and as the purposes of medicine, the especially application in preparing the medicine preventing and treating various influenza virus.Particularly, the present invention relates to logical compound shown in formula (I) or its stereoisomer, geometric isomer, dynamic isomer, nitrogen oxides, hydrate, solvate, metabolite, pharmaceutically acceptable salt or prodrug, each variable is defined as in the description.The invention still further relates to the salt purposes as medicine of logical compound shown in formula (I) or its stereoisomer, geometric isomer, dynamic isomer, nitrogen oxides, hydrate, solvate, metabolite, pharmaceutically acceptable salt or prodrug, especially as being used for preventing and treating the purposes of the medicine of influenza virus.

Description

Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes
Technical field
The present invention relates to a kind of pyrazine compounds containing cyclobutane substituent and the purposes as medicine thereof, it is anti-that the compounds of this invention is applicable to preparation The purposes of virus drugs.The invention still further relates to comprise the pharmaceutical composition of these compounds, and be directed to use with described compound, be used alone or with Other drug combination use, and prevent and treat the application of various virus, especially prevent and treat the application of influenza virus.
Background technology
Influenza (influenza is called for short influenza) is the ARI that influenza virus causes, and is also that a kind of infectiousness is strong, propagates speed Spend fast disease.It is mainly by the spittle, interpersonal contact or the contact transmission with contaminated article in air.Typical clinical symptoms It is: anxious high heat, overall pain, the most weak and slight respiratory symptom.General autumn and winter season is its high-incidence season, and caused complication is with dead Phenomenon of dying is the most serious.
Research to anti-influenza virus medicament in recent years achieves greater advance, and different anti-influenza virus medicaments effect in preventing and treating influenza is different. M2 ionophorous protein inhibitor (such as Buddha's warrior attendant gastral cavity amine and Rimantadine) is the influenza clinical treatment medicine listed the earliest, but there is resistance strain ratio Rate increase and to Type B influenza virus unit effect property limitation.It is to explore resisiting influenza virus herbal medicine thing at present that neuraminidase inhibitor is succeeded in developing Breakthrough in research, all has inhibitory activity to first, influenza B virus, and such as, Oseltamivir is prevention and treatment bird flu and people occurs First medication when parainfluenza is popular, but, in recent years, researcher all over the world be found that successively Oseltamivir is created resistance H1N1, H5N1, H3N2 and Type B influenza virus.Other anti-influenza virus medicament roots the most all have preferably to the inhibitory action of virus and antiviral Application prospect, but often need demonstration further.
Owing to the extensively application clinically of existing medicine makes influenza virus morph, these medicines are created drug resistance in various degree.Cause These novel anti-influenza virus medicament research and development are very urgent.
The invention provides a kind of antiviral agent, it has prevention effect and therapeutic action to various viruses, especially influenza virus.
Summary of the invention
The present invention relates to a kind of substituted pyrazine compounds of novel cyclobutane and its pharmaceutical composition, and prevent in preparation and treat various disease Purposes in poison, especially influenza virus medicine.
On the one hand, the present invention relates to a kind of compound, it is the compound as shown in formula (I), or its stereoisomer, geometric isomer, change Isomers, nitrogen oxides, hydrate, solvate, metabolite, pharmaceutically acceptable salt or prodrug,
Wherein, R1For hydroxyl, C1-3Hydroxy alkyl, C1-3Alkyl, C1-3Alkoxyl, amino or C1-3Alkylamino；
R2For-CN ,-C (=NH) NH2,-C (=O) NH2,-C (=S) NH2,-C (=NH) NHOH ,-C (=NH) NH-C1-3Alkyl or-C (=O) O-C1-3 Alkyl；
R3For hydrogen or C1-3Alkyl；
R4For hydrogen, hydroxyl, amino, C1-3Alkylamino, C1-3Alkoxyl, C1-3Alkyl, F, Cl, Br or I；With
R5For hydrogen, hydroxyl, C1-3Alkyl or C1-3Hydroxy alkyl.
In some embodiments, wherein
R1For hydroxyl, hydroxymethyl, hydroxyethyl, 2-hydroxypropyl, methyl, ethyl, n-pro-pyl, isopropyl, methoxyl group, ethyoxyl, ammonia Base, N-methylamino, N-ethylamino or N, N-dimethylamino；
R2For-CN ,-C (=NH) NH2,-C (=O) NH2,-C (=S) NH2,-C (=NH) NHOH ,-C (=NH) NHCH3、 -C (=NH) NHCH2CH3,-C (=O) OCH3Or-C (=O) OCH2CH3；
R3For hydrogen, methyl or ethyl；
R4For hydrogen, hydroxyl, amino, N-methylamino, N-ethylamino, N, N-dimethylamino, methoxyl group, ethyoxyl, methyl, ethyl, F, Cl, Br or I；With
R5For hydrogen, hydroxyl, methyl, ethyl, n-pro-pyl, isopropyl, hydroxymethyl, hydroxyethyl or 2-hydroxypropyl.
The present invention relates to compound or its stereoisomer of one of, geometric isomer, dynamic isomer, nitrogen oxides, hydrate, Solvate, metabolite, pharmaceutically acceptable salt or prodrug,
On the one hand, present invention also offers a kind of pharmaceutical composition containing compound of the present invention, this pharmaceutical composition contains of the present invention Compound, and pharmaceutically acceptable carrier, excipient, diluent, assistant agent, medium or combinations thereof.
In certain embodiments, it further comprises other anti-influenza virus medicament, and wherein said anti-influenza virus medicament is para rice Wei, zanamivir, Oseltamivir, that Ni Na meter Wei, Favipiravir, amantadine, Rimantadine, arbidol, Ribavirin, Si Tafu Woods, ingavirin (Ingavirin), GR-217029 or a combination thereof.
On the other hand, present invention also offers described compound or described pharmaceutical composition in preparation for preventing, process, treat or alleviate patient's stream Purposes in Influenza Virus medicine.
Preparation that another aspect of the present invention relates to the compound that formula (I) is comprised, the method separating and purifying.
The present invention also comprises compound and the stereoisomer thereof of the present invention, geometric isomer, dynamic isomer, nitrogen oxides, hydrate, solvent The application of compound, metabolite, or its pharmaceutically acceptable salt, the compound of the present invention is producing the application effectively suppressed in influenza virus.This The compound of invention is equally used for producing a kind of pharmaceuticals for alleviating, and stops, controls or treat patient's influenza infection.The present invention comprises medicine Composition, this pharmaceutical composition includes the compound representated by formula (I) and at least one pharmaceutically acceptable carrier, the combination institute of assistant agent or diluent Need effectively treats consumption.
The present invention comprises the disease of effective influenza virus, or the method sensitive to this illness equally, and the method comprises compound representated by use formula (I) Therapeutically effective amount patient is treated.
Unless other aspects show, all of stereoisomer of compound of the present invention, geometric isomer, dynamic isomer, nitrogen oxides, hydration Thing, solvate, metabolite, salt and pharmaceutically acceptable prodrug broadly fall into the scope of the present invention.
Specifically, salt is pharmaceutically acceptable salt.Term " pharmaceutically acceptable " includes that material or composition must be to be suitable for chemistry or toxicity Learn, relevant with other components of composition preparation and the mammal for treating.
The salt of the compound of the present invention also includes for preparing or purify what compound shown in the intermediate of compound shown in formula (I) or formula (I) separated The salt of enantiomter, but it is not necessarily pharmaceutically acceptable salt.
If the compound of the present invention is alkaline, the most conceivable salt can be prepared by any suitable method provided on document, such as, Use inorganic acid, example hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid and phosphoric acid etc..Or use organic acid, such as acetic acid, maleic acid, butanedioic acid, flat Peach acid, fumaric acid, malonic acid, pyruvic acid, malic acid, Lactic acid citric acid, oxalic acid, glycolic and salicylic acid；Pyrans saccharic acid, as Glucuronic acid and galacturonic acid；Alpha-hydroxy acid, such as citric acid and tartaric acid；Amino acid, such as asparatate and glutamic acid；Aromatic acid, as Benzoic acid and cinnamic acid；Sulfonic acid, such as p-methyl benzenesulfonic acid, benzene sulfonic acid, methanesulfonic acid, ethyl sulfonic acid, TFMS etc. or combinations thereof.
If the compound of the present invention is acid, the most conceivable salt can be prepared by suitable method, e.g., uses inorganic base or organic Alkali, such as ammonia (primaquine, parahelium, tertiary ammonia), alkali metal hydroxide, ammonium, N+(R14)4Salt and alkaline earth metal hydroxide, etc..Properly Salt include, but is not limited to, the organic salt obtained from amino acid, such as glycine and arginine, ammonia, such as primaquine, parahelium and tertiary ammonia, N+(R14)4 Salt, such as R14It is H, C1-4Alkyl, C6-10Aryl, C6-10Aryl C1-4Alkyl etc., and ring-type ammonia, such as piperidines, morpholine and piperazine etc., and from sodium, Calcium, potassium, magnesium, manganese, iron, copper, zinc, aluminium and lithium obtain inorganic salts.Also include that suitable, nontoxic ammonium, quaternary ammonium salt and gegenions are formed Amine cation, such as halide, hydroxide, carboxylate, hydrosulphate, phosphoric acid compound, nitric acid compound, C1-8Azochlorosulfonate acid compound and aromatic sulphonic acid Compound.
Detailed description of the invention book
Definition and general terms
Certain embodiments of the present invention be will now be described in more detail, and the example is by the structural formula enclosed and chemical formula explanation.The invention is intended to contain all of Substituting, revise and equivalent technical solutions, they are included in as in the scope of the invention of claim definition.Those skilled in the art will appreciate that Many similar with described herein or that be equal to method and material can be used in putting into practice the present invention.The present invention is not limited to method described herein and material. One or more of the document combined, patent and similar material different from the application or conflicting in the case of (include but not limited to defined Term, term application, described technology, etc.), it is as the criterion with the application.
It will further be appreciated that some feature of the present invention, for clearly visible, it is described in multiple independent embodiments but it also may Single embodiment provides in combination.Otherwise, the various features of the present invention, for brevity, it is described in single embodiment, But can also individually or provide with the sub-portfolio being arbitrarily suitable for.
Unless otherwise indicated, all scientific and technical terminologies used in the present invention have with those skilled in the art of the invention be generally understood that identical containing Justice.The all patents that the present invention relates to and public publication are integrally incorporated the present invention by reference.
Unless otherwise indicated, it should apply following definition used herein.For purposes of the present invention, chemical element and periodic table of elements CAS version, " Handbook of Chemistry and Physics ", the 75th edition, 1994 is consistent.Additionally, organic chemistry General Principle refers to " Organic Chemistry ", Thomas Sorrell, University Science Books, Sausalito:1999, and " March's Advanced Organic Chemistry " by Michael B. Smith and Jerry March, the description in John Wiley&Sons, New York:2007, entire contents is incorporated herein by.
Except as otherwise noted or having significantly conflict in context, article used herein " ", " one (kind) " and " described " are intended to include " extremely Few one " or " one or more ".Therefore, these articles used herein refer to the article of one or more than one (i.e. at least one) object. Such as, " component " refers to one or more component, it is possible to have more than one component be taken into account in the embodiment of described embodiment employing or Use.
Term used in the present invention " study subject " refers to animal.The most described animal is mammal.Study subject, the most also refers to primate Animal (the such as mankind, sex), ox, sheep, goat, horse, dog, cat, rabbit, rat, mouse, fish, bird etc..Implement at some In scheme, described study subject is primate.In other embodiments, described study subject is people.
The present invention says that the term " patient " of use refers to people's (including adult and children) or other animals.In some embodiments, " patient " is Refer to people.
It is open language that term " comprises ", i.e. includes the content specified by the present invention, but is not precluded from otherwise content.
Stereochemical definitions used in the present invention and rule typically follow S.P.Parker, Ed., McGraw-Hill Dictionary of Chemical Terms(1984)McGraw-Hill Book Company,New York；and Eliel,E.and Wilen,S.,“Stereochemistry of Organic Compounds”,John Wiley&Sons,Inc.,New York,1994。
Many organic compounds exist with optical active forms, and i.e. they have the ability making the plane of linearly polarized light rotate.Optics is being described During reactive compound, prefix D and L or R and S is used to represent the molecule absolute configuration about one or more chiral centre.Prefix d and l or (+) (-) be for caused by appointed compound linearly polarized light rotate symbol, wherein (-) or l represent that compound is left-handed.Prefix be (+) or the chemical combination of d Thing is dextrorotation.A kind of concrete stereoisomer is enantiomter, and the mixture of this isomers is referred to as enantiomeric mixture.Mapping is different The 50:50 mixture of structure body is referred to as racemic mixture or racemic modification, when chemical reaction or during there is no stereoselectivity or stereospecificity Time, may occur in which this situation.
The present invention come into the open compound any asymmetric atom (such as, carbon etc.) can presented in racemic or enantiomer are enriched with, such as (R)-, (S)-or the existence of (R, S)-configuration.In certain embodiments, each asymmetric atom has at least 50% enantiomer mistake in terms of (R)-or (S)-configuration Amount, at least 60% enantiomeric excess, at least 70% enantiomeric excess, at least 80% enantiomeric excess, at least 90% enantiomeric excess, at least 95% is right Reflect body excess, or at least 99% enantiomeric excess.
According to starting material and the selection of method, the compounds of this invention can disappear such as outward with in possible isomers or their mixture Presented in rotation body and non-enantiomer mixture (this depends on the quantity of asymmetric carbon atom).Optically active (R)-or (S)-isomers can Use chiral synthon or chiral reagent to prepare, or use routine techniques to split.If compound contains a double bond, substituent may be E or Z structure Type；If containing dibasic cycloalkyl in compound, the substituent of cycloalkyl may have cis or trans configuration.
The mixture of any stereoisomer of gained can be separated into pure or the purest geometry according to the difference in component physicochemical properties Isomers, enantiomter, diastereoisomer, such as, by chromatography and/or Steppecd crystallization.
By known method, the racemic modification of any gained end-product or intermediate can be split into light by method familiar to the person skilled in the art Learn enantiomer, e.g., by its diastereoisomeric salt obtained is separated.Racemic product can also be separated by chiral chromatogram, e.g., Use the high performance liquid chromatography (HPLC) of chiral sorbent.Especially, enantiomter can be prepared by asymmetric syntheses, such as, refers to Jacques,et al.,Enantiomers,Racemates and Resolutions(Wiley Interscience,New York,1981)；Principles of Asymmetric Synthesis(2ndEd.Robert E.Gawley,Jeffrey Aubé,Elsevier,Oxford,UK,2012)；Eliel,E.L. Stereochemistry of Carbon Compounds(McGraw-Hill,NY,1962)；Wilen,S.H.Tables of Resolving Agents and Optical Resolutions p.268(E.L.Eliel,Ed.,Univ.of Notre Dame Press,Notre Dame,IN 1972)；Chiral Separation Techniques:A Practical Approach(Subramanian,G.Ed.,Wiley-VCH Verlag GmbH&Co.KGaA,Weinheim, Germany,2007)。
At each several part of this specification, the come into the open substituent of compound of the present invention is open according to radical species or scope.Particularly pointing out, the present invention includes Each independent sub-combinations thereof of each member of these radical species and scope.Such as, term " C1-6Alkyl " refer in particular to individually disclosed methyl, Ethyl, C3Alkyl, C4Alkyl, C5Alkyl and C6Alkyl.
At each several part of the present invention, describe connection substituent.When this structure clearly needs linking group, for the Ma Ku cited by this group Assorted variable is interpreted as linking group.Such as, if this structure needs linking group and the Ma Kushi group definition for this variable to list " alkane Base " or " aryl ", then it should be understood that be somebody's turn to do " alkyl " or " aryl " to represent alkylidene group or the arylene group of connection respectively.
Terminology used in the present invention " alkyl " or " alkyl group ", represent and contain 1 to 20 carbon atom, saturated straight or branched univalent hydrocarbyl group, Wherein, the substituent that described alkyl group can optionally be described by one or more present invention replaces.Unless otherwise detailed instructions, alkyl group Containing 1-20 carbon atom.In one embodiment, alkyl group contains 1-12 carbon atom；In another embodiment, alkyl group contains 1-6 Individual carbon atom；In yet another embodiment, alkyl group contains 1-4 carbon atom；The most in one embodiment, to contain 1-3 carbon former for alkyl group Son.
The example of alkyl group comprises, but is not limited to, methyl (Me ,-CH3), ethyl (Et ,-CH2CH3), n-pro-pyl (n-Pr ,-CH2CH2CH3), Isopropyl (i-Pr ,-CH (CH3)2), normal-butyl (n-Bu ,-CH2CH2CH2CH3), isobutyl group (i-Bu ,-CH2CH(CH3)2), sec-butyl (s-Bu, -CH(CH3)CH2CH3), the tert-butyl group (t-Bu ,-C (CH3)3), n-pentyl (-CH2CH2CH2CH2CH3), etc..
Term " hydroxy alkyl " groups is separately replaced by one or two oh group, and wherein alkyl group has as described in the present invention Implication, the example of hydroxyalkyl groups includes, but is not limited to, hydroxymethyl (HOCH2-), hydroxyethyl (HOCH2CH2-), 2-hydroxypropyl (CH3CHOHCH2-), etc..
Term " alkoxyl " represents that alkyl group is connected with molecule remainder by oxygen atom, and wherein alkyl group has implication as described in the present invention. Unless otherwise detailed instructions, described alkoxy base contains 1-12 carbon atom.In one embodiment, alkoxy base contains 1-6 carbon atom； In another embodiment, alkoxy base contains 1-4 carbon atom；In yet another embodiment, alkoxy base contains 1-3 carbon atom.Institute State the substituent that alkoxy base can optionally describe by one or more present invention to replace.
The example of alkoxy base includes, but is not limited to, methoxyl group (MeO ,-OCH3), ethyoxyl (EtO ,-OCH2CH3), 1-propoxyl group (n-PrO, n-propoxyl group ,-OCH2CH2CH3), 2-propoxyl group (i-PrO, i-propoxyl group ,-OCH (CH3)2), 1-butoxy (n-BuO, n-fourth oxygen Base ,-OCH2CH2CH2CH3), 2-methyl-l-propoxyl group (i-BuO, i-butoxy ,-OCH2CH(CH3)2), etc..
Term " alkyl amino " or " alkylamino " include " N-alkyl amino " and " N, N-dialkyl amido ", and wherein amino group is separately by one Or two alkyl groups are replaced.Some of them embodiment is, alkyl amino is one or two C1-6The lower level that alkyl is connected on nitrogen-atoms Alkylamino group.Other embodiment is, alkyl amino is C1-3The alkylamino group of lower level.Suitably alkylamino group is permissible Being alkyl monosubstituted amino or dialkyl amido, such example includes, but is not limited to, N-methylamino, N-ethylamino, N, N-dimethylamino, etc..
Term " halogen " refers to fluorine (F), chlorine (Cl), bromine (Br) or iodine (I).
Unless other aspects show, structural formula described in the invention include all of isomeric forms (such as enantiomerism, diastereo-isomerism, and several What isomery (or conformational isomerism)): such as contain R, S configuration of asymmetric center, (Z), (E) isomers of double bond, and (Z), the rotamer of (E). Therefore, the single three-dimensional chemical isomer of the compound of the present invention or its enantiomter, diastereoisomer, or geometric isomer (or conformational isomerism Body) mixture broadly fall into the scope of the present invention.
Unless other aspects show, structural formula described in the invention and described compound include that all of isomeric forms is (such as enantiomerism, non- Enantiomerism, geometrical isomerism or conformational isomerism), nitrogen oxides, hydrate, solvate, metabolite, pharmaceutically acceptable salt and prodrug. Therefore, the single three-dimensional chemical isomer of the compound of the present invention, enantiomter, diastereoisomer, geometric isomer, rotamer, The compound of nitrogen oxides, hydrate, solvate, metabolite, pharmaceutically acceptable salt and prodrug falls within the scope of the present invention.It addition, Unless other aspects show, the structural formula of compound described in the invention includes the enriched isotope of one or more different atom.
Term used in the present invention " prodrug ", represents a compound and is converted into the compound shown in formula (I) in vivo.Such conversion is by precursor Medicine hydrolyze in blood or blood or tissue in through enzymatic conversion be the impact of precursor structure.Pro-drug compounds of the present invention can be ester, In existing invention, ester can have phenyl ester class, aliphatic (C as pro-drug1-24) esters, pivaloyloxymethyl esters, carbonic ester, carbamic acid Esters and amino acid esters.A such as compound in the present invention comprises hydroxyl, i.e. can be acylated the compound obtaining prodrug form. Other prodrug form includes phosphate, if these phosphate compounds are that the di on parent obtains.About pro-drug Complete discussion is referred to documents below: T.Higuchi and V.Stella, Pro-drugs as Novel Delivery Systems, Vol.14of the A.C.S.Symposium Series,Edward B.Roche,ed.,Bioreversible Carriers in Drug Design,American Pharmaceutical Association and Pergamon Press,1987,J.Rautio et al.,Prodrugs:Design and Clinical Applications,Nature Review Drug Discovery,2008,7,255-270,and S.J.Hecker et al.,Prodrugs of Phosphates and Phosphonates,Journal of Medicinal Chemistry,2008,51,2328-2345。
" metabolite " refers to that concrete compound or its salt is in vivo by the product obtained by metabolism.The metabolite of one compound is permissible Being identified by technology known to art, its activity can be adopted as passing through as described in the present invention and experimentally be characterized. Such product can be that esterification, degreasing, enzyme splits by passing through oxidation, reduction, hydrolysis, amidated, desamido-effect to drug compound Solution etc. method obtains.Correspondingly, the present invention includes the metabolite of compound, is fully contacted one including by the compound of the present invention and mammal Metabolite produced by the section time.
The definition of neutral body of the present invention chemistry and the use of convention are typically referenced to documents below: S.P.Parker, Ed., McGraw-Hill Dictionary of Chemical Terms(1984)McGraw-Hill Book Company,New York；and Eliel,E.and Wilen,S.,"Stereochemistry of Organic Compounds ", John Wiley&Sons, Inc., New York, the compound of 1994. present invention can comprise asymmetric center or chirality , therefore there is different stereoisomers in center.The all of stereoisomeric forms in any ratio of compound of the present invention, include, but not limited to, diastereomer, Enantiomter, atropisomer, and their mixture, such as racemic mixture, constitute the part of the present invention.A lot of organic compounds All existing with optical active forms, i.e. they are had the ability the plane of Plane of rotation polarised light.Describe optically active compound time, prefix D, L or R, S is used for representing the absolute configuration at molecular chiral center.Prefix d, l or (+), (-) are used for the symbol naming compound linearly polarized light to rotate, (-) or l be Refer to compound be left-handed, prefix (+) or d refer to that compound is dextrorotation.The chemical constitution of these stereoisomers is identical, but they Stereochemical structure is different.Specific stereoisomer can be enantiomer, and the mixture of isomers is commonly referred to enantiomeric mixture.50:50 Mixture of enantiomers be referred to as racemic mixture or racemic modification, this may cause not having in chemical reaction process stereoselectivity or stereotaxis Property.Term " racemic mixture " and " racemic modification " refer to the mixture of equimolar two enantiomters, lack optical activity.
Stereoisomer " refer to that there is identical chemical constitution, but the compound that atom or group spatially arrangement mode is different.Stereoisomer bag Include enantiomter, diastereoisomer, rotamer (rotational isomer), geometric isomer (cis/trans) isomers, atropisomer, Etc..
Term " dynamic isomer " or " tautomeric form " refer to that the isomer of the structure of different-energy can be converted mutually by low energy barrier. Such as proton tautomer (the most prototropic dynamic isomer) includes the change migrated by proton, same such as keto-enol and imine-enamine Divide isomerization.Valence (chemical valence) dynamic isomer includes the change reassembling into bonding electron.
" chirality " is that have can not the molecule of overlapping character with its mirror image；And " achirality " refer to can be overlapping with its mirror image molecule.
" enantiomter " refer to two of a compound can not be overlapping but be mutually the isomers of mirror.
" diastereoisomer " refers to two or more chiral centre and the stereoisomer of its molecule mirror image the most each other.Diastereoisomer has Different physical propertys, such as fusing point, boiling point, spectral quality and reactivity.Non-enantiomer mixture can be operated such as electrophoresis by high resolution analysis With chromatogram, such as HPLC separate.
" pharmaceutically acceptable salt " used in the present invention refers to organic salt and the inorganic salts of the compound of the present invention.Pharmaceutically acceptable salt is in institute Genus field is known to us, such as document: S.M.Berge et al., describe pharmaceutically acceptable salts in detail in J. Described in Pharmaceutical Sciences, 1977,66:1-19..The salt that pharmaceutically acceptable nontoxic acid is formed includes, but is not limited to, React, with amino group, the inorganic acid salt formed and have hydrochloride, hydrobromate, phosphate, sulfate, perchlorate, and acylate such as acetate, Oxalates, maleate, tartrate, citrate, succinate, malonate, or by additive method described on books document such as Ion-exchange obtains these salt.Other pharmaceutically acceptable salts include adipate, alginates, ascorbate, aspartate, benzene Sulfonate, benzoate, bisulphate, borate, butyrate, camphor hydrochlorate, camsilate, cyclopentyl propionate, digluconate, Lauryl sulfate, esilate, formates, fumarate, gluceptate, glycerophosphate, gluconate, Hemisulphate, Enanthate, caproate, hydriodate, 2-hydroxy-ethanesulfonate salt, lactobionate, lactate, laruate, lauryl sulfate, malic acid Salt, malonate, mesylate, 2-naphthalene sulfonate, nicotinate, nitrate, oleate, palmitate, pamoate, pectate, over cure Hydrochlorate, 3-phenylpropionic acid salt, picrate, pivalate, propionate, stearate, rhodanate, tosilate, undecylate, Valerate, etc..The salt obtained by suitable alkali includes alkali metal, alkaline-earth metal, ammonium and N+(C1-4Alkyl)4Salt.The present invention is also intended to design The quaternary ammonium salt that the compound of the group of any comprised N is formed.Water solubility or oil-soluble or dispersion product can be obtained by quaternization. Alkali metal or alkali salt include sodium, lithium, potassium, calcium, magnesium, etc..Pharmaceutically acceptable salt farther includes suitable, nontoxic ammonium, The amine cation that quaternary ammonium salt and gegenions are formed, such as halide, hydroxide, carboxylate, hydrosulphate, phosphoric acid compound, nitric acid compound, C1-8Azochlorosulfonate acid compound and aromatic sulphonic acid compound.
" hydrate " of the present invention refers to that solvent molecule is the associated matter that water is formed.
" nitrogen oxides " of the present invention refer to when compound containing several amine functional group time, can by 1 or more than 1 nitrogen-atoms oxidation formed N-oxide. The particular example of N-oxide is N-oxide or the N-oxide of nitrogen heterocyclic ring nitrogen-atoms of tertiary amine.Available oxidant such as hydrogen peroxide or peracid (such as peroxycarboxylic acid) processes corresponding amine formation N-oxide and (sees Advanced Organic Chemistry, Wiley Interscience, the 4th Version, Jerry March, pages).Especially, N-oxide can be prepared (Syn.Comm.1977,7,509-514) by the method for L.W.Deady, its In such as in atent solvent such as dichloromethane, make amines react with m-chloroperoxybenzoic acid (MCPBA).
" solvate " of the present invention refers to the associated matter that the compound of one or more solvent molecule and the present invention is formed.Form the molten of solvate Agent includes, but is not limited to, water, isopropanol, ethanol, methyl alcohol, dimethyl sulfoxide, ethyl acetate, acetic acid and ethylaminoethanol.Term " hydrate " Refer to that solvent molecule is the associated matter that water is formed.
As used in the present invention any disease or illness " treated " in term, wherein some embodiment middle fingers improve disease or illness (i.e. slow down or Stop or palliate a disease or the development of its at least one clinical symptoms).In other embodiments, " treat " refer to relax or improve at least one health Parameter, including the body parameter may not discovered by patient.In other embodiments, " treat " refer to from health (such as stable discernable Symptom) or physiologically (such as stablize the parameter of health) or above-mentioned two aspects regulation diseases or illnesss.In other embodiments, " treat " Refer to prevention or postpone disease or the outbreak of illness, occur or deteriorate.
Any structural formula that the present invention is given is also intended to represent that these compounds are not by form and the form of isotope enrichment of isotope enrichment.Coordination The compound of element enrichment has the structure that the formula that the present invention provides is described, except one or more atoms are had selected atomic weight or mass number Atom is replaced.The Exemplary isotopes in the compounds of this invention can be introduced and include the isotope of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulphur, fluorine and chlorine, as2H,3H,11C,13C,14C,15N,17O,18O,18F,31P,32P,35S,36Cl and125I。
On the other hand, compound of the present invention includes compound defined in the present invention of isotope enrichment, such as, wherein there is radioactivity coordination Element, as3H,14C and18Those compounds of F, or wherein there is non radioactive isotope, as2H and13C.The change of such isotope enrichment Compound can be used for metabolism research and (uses14C), kinetics research (uses such as2H or3H), detect or imaging technique, as positron emission is broken Layer scanning (PET) or include the SPECT (SPECT) of medicine or substrate tissue measure of spread, or can be used for suffering from In the radiotherapy of person.18The compound of F enrichment is especially desirable for PET or SPECT studies.Compound shown in the formula (I) of isotope enrichment Suitable isotope can be used to mark by the embodiment in routine techniques familiar to the person skilled in the art or the present invention and preparation process are described Reagent substitutes original used unmarked reagent to be prepared.
On the other hand, the present invention relates to prepare the intermediate of the compound that formula (I) is comprised.
On the other hand, the preparation that the present invention relates to the compound that formula (I) is comprised, the method separating and purifying.
On the other hand, the present invention provides a kind of pharmaceutical composition, and described pharmaceutical composition comprises the compounds of this invention, pharmaceutically acceptable carrier, Excipient, diluent, assistant agent, solvent, or combinations thereof.In some embodiments, pharmaceutical composition can be liquid, solid, semi-solid, Gel or spray-type.
Additionally, higher isotope particularly deuterium is (i.e.,2H or D) replacement can provide some treat advantage, these advantages are by metabolic stability more Height brings.Such as, Half-life in vivo increases or volume requirements reduces or therapeutic index obtains improving and brings.Should be appreciated that the deuterium in the present invention It is seen as the substituent of formula (I) compound.The concentration of such higher isotope particularly deuterium can be defined by the isotope enrichment factor.Institute of the present invention The term " the isotope enrichment factor " used refers to specified ratio between isotopic isotope abundance and natural abundance.If the compounds of this invention Substituent be designated as deuterium, this compound for each D-atom specified, have at least 3500 (each specify the deuterium of 52.5% at D-atom to mix), At least 4000 deuterium of 60% (mix), at least 4500 (deuterium of 67.5% mixes), at least 5000 (deuterium of 75% mixes), at least 5500 (deuterium of 82.5% is mixed Enter), at least 6000 deuterium of 90% (mix), at least 6333.3 (deuterium of 95% mixes), at least 6466.7 (deuterium of 97% mixes), at least 6600 (99% Deuterium mix) or the isotope enrichment factor of at least 6633.3 deuterium of 99.5% (mix).The pharmaceutically useful solvate of the present invention includes wherein recrystallisation solvent Can be the substituted such as D of isotope2O, acetone-d6、DMSO-d6Those solvates.
The compounds of this invention and pharmaceutical composition, preparation and administration
Described pharmaceutical composition comprises the compound of any present invention.This pharmaceutical composition can also comprise pharmaceutically acceptable load further Body, excipient, diluent, assistant agent, medium or a combination thereof.
Described pharmaceutical composition comprises the medicine of resisiting influenza virus further.The medicine of described resisiting influenza virus can be any of to be different from this Other of bright compound are for the medicine of anti influenza.For example, it is possible to draw for Peramivir, zanamivir, Oseltamivir, that Ni Na meter Wei, method Wei, amantadine, Rimantadine, arbidol, Ribavirin, Si Tafulin, ingavirin (Ingavirin), GR-217029 or a combination thereof.
When can be used for treatment, the compounds of this invention of therapeutically effective amount, especially formula (I) compound and pharmaceutically acceptable salt thereof can be as not The chemicals of processing gives, and the active component being alternatively arranged as pharmaceutical composition provides.Therefore, present invention also provides for pharmaceutical composition, this medicine Compositions includes this compounds of this invention of therapeutically effective amount, especially formula (I) compound or its pharmaceutically acceptable salt and one or more medicines Acceptable carrier, diluent or excipient on.Term as used herein " therapeutically effective amount " refers to be enough to demonstrate significant patient benefit The total amount of each active component of (such as viral load minimizing).When using single active component individually dosed, this term only refers to this composition.When During combination application, no matter this term then refers to combination, when being sequentially or simultaneously administered, all cause the combined amount of the active component of result for the treatment of.The present invention Compound, especially formula (I) compound and pharmaceutically acceptable salt thereof are described above.From compatible with other compositions of preparation and to its recipient without From the point of view of in the sense that evil, carrier, diluent or excipient must be acceptable.According to the another aspect of present invention, also provide for for preparing The method of pharmaceutical preparation, the method includes the compounds of this invention, especially formula (I) compound or its pharmaceutically acceptable salt and one or more Pharmaceutically acceptable carrier, diluent or excipient mixing.Term used in the present invention " pharmaceutically acceptable " refer to such compound, Raw material, composition and/or formulation, they rational medicine judge in the range of, it is adaptable to patient tissue contacts and without excessive toxicity, excitant, Allergy or the other problems symmetrical with rational interests/Hazard ratio and complication, and it is effective to given application.
Pharmaceutical preparation can be in unit dosage forms, and each UD contains the active component of scheduled volume.The dosage level of the compound of present invention between Between about 0.01 mg/kg (mg/kg) body weight/day and about 250 mg/kg body weight/day, preferably in the range of about 0.05mg/kg body weight/day and about 100 Between mg/kg body weight/day, usually with monotherapy for preventing or treat the disease of influenza virus mediation.Generally can by every day about 1 to about 5 time or The pharmaceutical composition of present invention is given as continuous infusion.This kind of dose regimen can be used as long-term or short-term therapy.Mix with carrier material with preparation The amount of the active component of single formulation is by according to disease to be treated, the order of severity of disease, administration time, method of administration, the row of compound used therefor Let out speed, treatment time and patient age, sex, body weight and situation and change.Preferably unit dosage forms is the day containing hereinbefore active component Dosage or divided dose or the unit dosage forms of its appropriate fraction.The available low dose being already clearly below compound optimal dose starts treatment.Hereafter, with less Increment carry out escalated dose until reaching optimum efficiency in this case.It is said that in general, the concentration level most desirably giving compound is generally may be used There is provided effective result without regard to causing any harmful or poisonous side effect at anti-virus aspect.
When the composition of present invention comprises the combination of the compound of present invention and one or more other treatment medicines or prophylactic agent, change The dosage level of compound and other medicine, generally in monotherapy scheme, accounts for the about 10-150% of bio-occlusion pharmaceutical quantities, more preferably accounts for normal administration The about 10-80% of dosage.Pharmaceutical preparation is suitable to by any suitable administration, such as by oral (including oral cavity or sublingual), rectum, nose, Locally (include oral cavity, sublingual or percutaneous), vagina or parenteral (in including subcutaneous, intracutaneous, intramuscular, joint, in intrasynovial, breastbone, sheath In, in focus, intravenous or intradermal injection or infusion) approach.This kind of preparation can be prepared, such as by any known method of art of pharmacy By active component is mixed with carrier or excipient.Preferred oral is administered or drug administration by injection.
The pharmaceutical preparation being suitable to oral administration is provided by independent unit, such as capsule or tablet；Powder or granule；Aqueous or non-aqueous liquid In solution or supensoid agent；Edible foam formulations or foaming preparations (whip)；Or O/w emulsion agent or water in oil emulsion liquor.
For example, for oral administration in the form of a tablet or capsule, active medicine component can carry with pharmaceutically acceptable oral, non-toxic inertia Body (such as ethanol, glycerine, water etc.) mixes mutually.By compound powder being broken into suitable fine sizes, and with by as the pharmaceutical carrier pulverized Powder is prepared in (edible carbohydrate such as such as starch or mannitol etc.) mixing.Also can there is flavouring, preservative, dispersant and colouring agent.
By preparing pulverulent mixture as above, and it is loaded in the gelatin shell of shaping, prepares capsule.Before filling operation, can be by Glidant and lubricant (such as colloidal silica, talcum powder, magnesium stearate, calcium stearate or solid polyethylene glycol) are added in pulverulent mixture. Also can add and will improve disintegrant or the solubilizer (such as agar, calcium carbonate or sodium carbonate) of medicine utilizability when the lower capsule of clothes.
When needing in addition or be required, it is possible to suitable adhesive, lubricant, disintegrant and colouring agent are mixed in mixture.Suitably adhesive bag Include starch, gelatin, natural sugar (such as glucose or beta lactose), corn sweetener, natural and paragutta (such as gum arabic, the yellow alpine yarrow in west Glue or mosanom), carboxymethylcellulose calcium, polyethylene glycol etc..Lubricant for these formulations includes enuatrol, sodium chloride etc..Disintegrant includes But it is not limited to starch, methylcellulose, agar, bentonite, xanthans etc..Such as, by making pulverulent mixture, pelletize or pre-compressing tablet, add Enter lubricant and disintegrant, tabletted, thus make tablet.By the compound suitably pulverized and diluent as described above or base-material, optionally With adhesive (such as carboxymethylcellulose calcium, alginates, gelatin or polyvinylpyrrolidone), dissolving inhibitor (such as paraffin), absorption accelerator (quaternary salt) and/or absorbent (such as bentonite, kaolin or Dicalcium Phosphate) mix, and prepare pulverulent mixture.Useful binders (such as syrup, Starch slurry, mucialga of arabic gummy (acadiamucilage) or cellulosic material or polymeric material solution) pressurize and sieve, by pulverulent mixture system after wetting Grain.The alternative pelletized is, can be by pulverulent mixture by tablet press machine, and result is to smash the best agglomerate of formation to make particle again.Can By adding stearic acid, stearate, talcum powder or mineral oil make particle lubrication to prevent from adhering on the punch die of tablet press machine.Then by mixing through lubrication Compound tabletted.The compound of present invention also can mix with free-pouring inert carrier, it is not necessary to just can be pressed by granulation or pre-tableting step Make sheet.Can provide transparent or opaque and be polished clothing (polish coating of wax) by shellac seal coat, sugar-coat or polymeric material clothing and wax The protectiveness coating material of composition.Can be added to dyestuff in these coating materials distinguish different UDs.
Oral liquid such as solution, syrup and elixir can be prepared with dosage unit form, thus specified rate contains the compound of scheduled volume. Syrup can be prepared by being dissolved in the suitably seasoned aqueous solution by compound, and elixir can be prepared by using non-toxic vehicle.Also can add increasing Solvent and emulsifying agent (such as ethoxylated isostearyl alcohols and polyoxyethylene sorbitol ether), preservative, flavoring additive (such as peppermint oil or natural Sweetener or saccharin or other artificial sweeteners) etc..
If appropriate, the dosage unit preparations microencapsulation of oral administration can be used for.Also preparation can be made time delay or sustained release, such as By being coated or being embedded in the microparticle material such as polymer, wax.
The compounds of this invention, especially formula (I) compound and pharmaceutically acceptable salt thereof can give with liposome delivery systems, the least individual layer Liposome, big unilamellar liposome and multilamellar liposome.Liposome can be by multiple phosphatide (such as cholesterol, octadecylamine or phosphatid ylcholine) structure Become.
The compounds of this invention, especially formula (I) compound and pharmaceutically acceptable salt thereof also can be by using monoclonal antibody as single carrier (compound molecule is coupled) passs medicine.Compound also can with as can the soluble polymer coupling of target medicine carrier.This base polymer can wrap Include polyvinylpyrrolidone, pyran co-polymer, poly-hydroxypropyhnethacrylamide phenol, polyhydroxyethylaspart or taken by palmitoyl residues The polyethylene-oxide polylysine in generation.Additionally, compound can be for reaching the controlled release of medicine, this kind of with a class Biodegradable polymeric coupling Polymer such as PLA, poly-epsilon-caprolactone, poly butyric, poe, polyacetals, poly-dihydropyran, polybutylcyanoacrylate and water-setting The cross-linked copolymer of glue or amphipathic nature block polymer.
The pharmaceutical preparation being suitable to percutaneous dosing can be as discrete patch (discrete patch) to keep and recipient's epidermis close contact in a long time. Such as, active component can be passed medicine by by electro-ionic osmosis patch, generally can be found in Pharmaceutical Research 1986,3 (6), and 318.
The pharmaceutical preparation being suitable to topical can be made into ointment, cream, supensoid agent, lotion, powder, solution, paste, gel, spray Mist agent, aerosol, oil formulation or transdermal patch.
The pharmaceutical preparation being suitable to rectally can be as suppository or as enema offer.
The pharmaceutical preparation (wherein carrier is solid) being suitable to nose administration includes the dust base that particle diameter is such as 20-500 micrometer range, by with snuffing side Formula is administered, and is i.e. quickly sucked from close to the dust base container of nose by nasal passage.Wherein carrier is liquid, is adapted as nasal mist or drips The appropriate formulation that nose agent is administered includes aqueous solution agent or the oily solution agent of active component.
Be suitable to the pharmaceutical preparation by inhalation and include minuteness particle pulvis (dust) or mist agent (mist), the dosage pressure of available dissimilar metering Prepared by the device of contracting aerosol, nebulizer, insufflator or other matters delivery aerosol spray.
The pharmaceutical preparation being suitable to vagina administration can provide with vaginal plug, vagina plug, cream, creme, gel, paste, foaming agent or spray.
The pharmaceutical preparation being suitable to parenteral includes aqueous and non-aqueous sterile injection solution and aqueous and non-aqueous sterile suspensions, aqueous and non- Aqueous, sterile injection solution can contain antioxidant, buffer, bacteriostatic agent and make the described preparation solute isotonic with receptor's blood waiting, aqueous and Non-aqueous sterile suspensions can include suspending agent and thickener.Preparation can provide with UD or multi-dose container, and the peace such as sealed is triumphant and bottle, And under the conditions of freeze-drying (being lyophilized) can be saved in, only need to add sterile liquid carrier, such as water for injection before use.Face the injection of used time configuration Solution and supensoid agent can be prepared by sterile powder injection, granule and tablet.
It will be appreciated that in addition to the composition being particularly mentioned above, preparation also includes relevant with described preparation type commonly used in the art other Composition, this kind of preparation being for example suitable for oral administration can include flavouring.
The compounds of this invention and the purposes of pharmaceutical composition
The feature of the pharmaceutical composition of the present invention includes the compound of formula (I), the compound listed by the present invention, or the compound of embodiment 1-14, With pharmaceutically acceptable carrier, assistant agent, or excipient.In the composition of the present invention, compound can effectively suppress the ability of influenza virus, it is adaptable to The prevention of influenza virus and treatment.
Comprise the methods for the treatment of that the compounds of this invention or pharmaceutical composition are administered, farther include patient is administered the medicine of other anti influenza, thus, The medicine of the compound of the present invention Yu other anti influenza can be carried out therapeutic alliance, the medicine of wherein said anti influenza be Peramivir, zanamivir, Oseltamivir, that Ni Na meter Wei, Favipiravir, amantadine, Rimantadine, arbidol, Ribavirin, Si Tafulin, ingavirin (Ingavirin), GR-217029 or a combination thereof.
And comprise the methods for the treatment of that the compounds of this invention or pharmaceutical composition are administered, comprise the administration of other Tamiflu further, wherein, its His Tamiflu can with the compounds of this invention or its pharmaceutical composition administering drug combinations, the compounds of this invention or pharmaceutical composition as single formulation, Or separate compound or pharmaceutical composition are as a part for multi-form.Other Tamiflu can be administered simultaneously from the compounds of this invention or different Time be administered.The situation of the latter, administration can be staggered and be carried out such as 6 hours, 12 hours, 1 day, 2 days, 3 days, 1 week, 2 weeks, 3 weeks, 1 month or 2 Within individual month, carry out.
The compound of the present invention or " effective dose " of pharmaceutically acceptable composition or " effective dose " refer to process or alleviate one or more present invention It is previously mentioned the effective dose of the severity of illness.The method according to the invention, compound and composition can be any dosage and any method of administration It is efficiently used for the order of severity processing or palliating a disease.Situation according to patient is changed by required measuring accurately, and this depends on race, the age, The general condition of patient, the order of severity of infection, special factor, administering mode, etc..Compound or composition can with one or more its He is administered, as discussed in the present invention by therapeutic agent.
The general synthetic method of this compound
Usually, the compound of the present invention can be prepared by method described in the invention, unless there are further instruction, Qi Zhongqu Shown in the definition of Dai Ji such as formula (I).Following reaction scheme and embodiment are used for being further illustrated by present disclosure.
Those skilled in the art will realize that: chemical reaction described in the invention can be used to prepare suitably its of many present invention His compound, and other method of the compound for preparing the present invention is considered as within the scope of the present invention.Such as, according to this The synthesis of the compound inventing those non-illustrations can successfully be completed by method of modifying by those skilled in the art, such as suitable guarantor Protect interference group, by utilizing reagent known to other except described in the invention, or reaction condition is made the amendment of some routines.Separately Outward, reaction disclosed in this invention or known reaction condition are applicable to the preparation of other compounds of the present invention the most admittedly.
The embodiments described below, unless other aspects show all of temperature and are set to degree Celsius.Reagent is bought in goods providers such as Ling Kai Medicine, Aldrich Chemical Company, Inc., Arco Chemical Company and Alfa Chemical Company, do not have during use Have through being further purified, unless other aspects show.General reagent from Xi Long chemical plant, Shantou, Guangdong brilliance chemical reagent factory, extensively State chemical reagent factory, Tianjin Hao Yuyu Chemical Company, Qingdao Teng Long chemical reagent Co., Ltd, and Haiyang Chemical Plant, Qingdao buy Obtain.
Anhydrous tetrahydro furan is to be dried to obtain through metallic sodium backflow.Anhydrous methylene chloride and chloroform are to be dried to obtain through calcium hydride backflow. Ethyl acetate, DMA and petroleum ether are to be dried in advance through anhydrous sodium sulfate to use.
Hereinafter reaction is usually and overlaps a drying tube (unless other aspects show) under nitrogen or argon gas positive pressure or on anhydrous solvent, and reaction bulb is all Suitable rubber stopper beyond the Great Wall, substrate is squeezed into by syringe.Glassware is all dried.
Chromatographic column is to use silicagel column.Silica gel (300-400 mesh) is purchased from Haiyang Chemical Plant, Qingdao.NMR spectrum is with CDC13Or DMSO-d6 For solvent (report in units of ppm), with TMS (0 ppm) or chloroform (7.25ppm) as reference standard.When multiplet occurs when, Abbreviation by following for use: s (singlet, unimodal), d (doublet, bimodal), t (triplet, triplet), m (multiplet, multiplet), Br (broadened, broad peak), dd (doublet of doublets, quartet), dt (doublet of triplets, double triplets).Coupling constant, Represent with hertz (Hz).
Algorithm (MS) data are by being equipped with G1312A binary pump and the Agilent of a G1316A TCC (column temperature is maintained at 30 DEG C) The spectrometer of 6320 series LC-MS measures, and G1329A automatic sampler and G1315B DAD detector are applied to analyze, ESI source It is applied to LC-MS spectrometer.
Algorithm (MS) data are by being equipped with G1311A quaternary pump and the Agilent 6120 of G1316A TCC (column temperature is maintained at 30 DEG C) The spectrometer of series LC-MS measures, and G1329A automatic sampler and G1315D DAD detector are applied to analyze, and ESI source is applied to LC-MS spectrometer.
Both the above spectrometer is provided with Agilent Zorbax SB-C18 post, and specification is 2.1 × 30mm, 5 μm.Volume injected is to pass through Sample concentration determines；Flow velocity is 0.6mL/min；The peak value of HPLC is to record reading by the UV-Vis wavelength at 210nm and 254nm Take.Flowing is formic acid acetonitrile solution (phase A) and formic acid ultra-pure water solution (phase B) of 0.1% of 0.1% mutually.Condition of gradient elution is as shown in table 1:
Table 1
Time (min) A(CH3CN, 0.1%HCOOH) B(H2O, 0.1%HCOOH)
0-3 5-100 95-0
3-6 100 0
6-6.1 100-5 0-95
6.1-8 5 95
Compound is purified and is evaluated by Agilent 1100 series of high efficiency liquid chromatogram (HPLC), wherein UV detection at 210nm and At 254nm, Zorbax SB-C18 post, specification is 2.1 × 30mm, 4 μm, 10 minutes, and flow velocity is (the 0.1% of 0.6mL/min, 5-95% Formic acid acetonitrile solution) (0.1% aqueous formic acid), column temperature is maintained at 40 DEG C.
The use of brief word below runs through the present invention:
BOC, Boc tert-butoxycarbonyl
Bz benzoyl
NBu4Br TBAB
CDC13Deuterochloroform
CD3OD deuterated methanol
H2O water
Et3N triethylamine
EtOAc ethyl acetate
UL microlitre
ML, m milliliter
N2Nitrogen
NH2OH azanol
Pd/C palladium/carbon
PE petroleum ether (60 90 DEG C)
DCM dichloromethane
MeOH methyl alcohol
DMSO dimethyl sulfoxide
DMSO-d6Deuterated dimethyl sulfoxide
DMF N,N-dimethylformamide
DIPEA diisopropylethylamine
H2O2Hydrogen peroxide
THF oxolane
NaOMe sodium methoxide
NH4Cl ammonium chloride
H hour
TBAF tetrabutyl ammonium fluoride
HPLC high performance liquid chromatography
MS mass spectrum
POCl3POCl3
KF potassium fluoride
SOCl2Thionyl chloride
Pd/C palladium/carbon
NaOH NaOH
H2O2Hydrogen peroxide
Na2SO4.10H2O Disodium sulfate decahydrate
Min minute
Synthetic schemes
Synthetic method 1
Compound (c) can be prepared by synthetic method 1, wherein R1、R3、R4、R5There is implication of the present invention, compound (a) Under alkali (such as triethylamine, potassium carbonate etc.) acts on, target compound 3 is obtained by necleophilic reaction with compound (b).
Synthetic method 2
Compound (d) can be prepared by synthetic method 2, wherein R1、R3、R4、R5There is implication of the present invention, compound (c) Target compound (d) is obtained with azanol reaction.
Synthetic method 3
Compound (c) can be prepared by synthetic method 3, wherein R1、R3、R4、R5There is implication of the present invention, compound (c) React under the effect of alkali with ammonium chloride and obtain target compound (e).
Synthetic method 4
Compound (f) can be prepared by synthetic method 4, wherein R1、R3、R4、R5There is implication of the present invention, compound (c) Under alkalescence condition (such as potassium carbonate), reaction obtains target compound (f).
The synthesis of intermediate
Intermediate 1:3,6-bis-Calmazine-2-formonitrile HCN
The synthesis of step 1:6-bromo-3-HYDROXYPYRAZINE-2-formamide
2,6-lutidines (4.2mL, 35.97mmol, 1eq.) is joined 3-HYDROXYPYRAZINE-2-formamide (5g, 35.97mmol, 1eq.) and In DMF (50mL) solution of TBAB (19g, 39.57mmol, 1.1eq.), reactant liquor is stirred at room temperature 17h, after having reacted, In reaction system, add the sodium chloride solution of 10%, separate out solid, the solid of collection is joined 30mL water and the mixed solution of 30mL EtOAc In, it is stirred for ten minutes.The organic phase of isolated saturated aqueous common salt (20mL x 2) washs, then is dried with anhydrous sodium sulfate, reduced pressure concentration, Residue purifies through silica gel column chromatography column chromatography (eluant, eluent: PE/EtOAc (V/V)=2/1) and obtains titled compound as white solid (4g, 51%).
MS:(ESI,pos.ion)m/z:219.1[M+1]+.
The synthesis of step 2:6-bromo-3-chloropyrazine-2-formonitrile HCN
At 0 DEG C, by POCl3(5.3mL, 57.33mmol, 3.5eq.) and DIPEA (11.4mL, 65.52mmol, 4eq.) join the bromo-3-of 6- In chlorobenzene (70mL) solution of HYDROXYPYRAZINE-2-formamide (3.57g, 16.38mmol, 1eq.), reactant liquor is heated to 100 DEG C and stirs 17h, After having reacted, by reactant liquor reduced pressure concentration, the crude on silica gel column chromatography being concentrated to give (eluant, eluent: PE/EtOAc (V/V)=10/1) purifies To white solid title compound (2.2g, 62%).
ESI:(ESI,pos.ion)m/z:219.4[M+1]+.
The synthesis of step 3:3,6-bis-Calmazine-2-formonitrile HCN
By KF (5.7g, 98.44mmol, 3.7eq.) and NBu4Br (1.72g, 5.32mmol, 0.2eq.) joins in the anhydrous DMSO of 10mL, instead Answer mixture to be heated to 100 DEG C of reaction 1h, be then cooled to 70 DEG C, add compound 6-bromo-3-chloropyrazine-2-formonitrile HCN (5.8g, 26.6mmol, 1 Eq.) dry toluene (20mL) solution, gained mixture continues to react 3h at this temperature.After having reacted, add 10mL water, then separation has Machine phase and aqueous phase, the organic phase of separation saturated aqueous common salt 20mL washs, and organic phase adds concentrated hydrochloric acid and regulates to pH about 1.6, the most again with saturated Sodium chloride solution 20mL washs.Organic phase is dried with anhydrous sodium sulfate, reduced pressure concentration, and the thick product being concentrated to give is through silica gel column chromatography (wash-out Agent: PE/EtOAc (V/V)=30/1) purify obtain white solid title compound (1.85g, 49%).
1H NMR(400MHz,CDCl3):δ8.34-8.31(d,1H).
Intermediate 2:(3-(amino methyl)-3-hydroxycyclobutyl) methyl benzoic acid ester
The synthesis of step 1:3-oxygen cyclobutane-carboxylic acid methyl esters
0 DEG C and stirring under, by SOCl2(3.1mL, 0.045mol, 0.5eq.) joins 3-'s oxygen cyclobutane formate (10.0g, 0.09mol, 1.0eq.) CH3In OH (50mL) solution, reduced pressure concentration after reactant liquor backflow 4.0h, residue by silicagel column chromatography (eluant, eluent: PE/EtOAc (V/V) =10/1) purifying obtains colorless oil title compound (10.0g, 90%).
1H NMR(400MHz,CDCl3):δ3.77(s,3H),3.23-3.46(s,5H).
The synthesis of step 2:3,3-bi-methoxy cyclobutane-carboxylic acid methyl esters
Trimethyl orthoformate (25.7mL, 234.4mmol, 2eq.) and p-methyl benzenesulfonic acid (0.75g, 5%) are joined 3-oxygen cyclobutane-carboxylic acid methyl esters In the 50mL dichloromethane solution of (15g, 117.19mmol, 1eq.), reactant liquor is placed at 45 DEG C reaction 5h.After having reacted, add in reactant liquor Enter 50mL water, and extract with DCM (20mL x 2).The organic phase merged saturated aqueous common salt (40mL x 2) washs, then anhydrous sodium sulfate is done Dry, then filter, be finally concentrated under reduced pressure to give colorless oil title compound (19.6g, 96%).
Step 3:(3,3-bi-methoxy cyclobutane base) synthesis of methyl alcohol
At 0 DEG C, by anhydrous 3, THF (50mL) solution of 3-bi-methoxy cyclobutane-carboxylic acid methyl esters (18.6g, 106.78mmol, 1eq.) joins hydrogen Changing in anhydrous THF (150mL) suspension of lithium aluminium (4.06g, 106.78mmol, 1eq.), reactant mixture continues to stir 17h at 0 DEG C.So Afterwards toward the Na adding 1mL in reactant liquor2SO4.10H2The saturated solution of O, refilters, and filtrate is through being concentrated under reduced pressure to give colorless oil title compound (10.7g, 69%).
1H NMR(400MHz,CD3OD):δ3.60(d,2H),3.12(s,3H),3.10(s,3H),2.27(m,1H),2.24(s,1H),2.23(m, 2H)；1.85(m,2H).
Step 4:(3,3-bi-methoxy cyclobutane base) synthesis of methyl benzoic acid ester
(3,3-bi-methoxy cyclobutane base) methyl alcohol (10.7g, 73.2mmol, 1eq.) is dissolved in DCM (100mL), and is added thereto to triethylamine (25.4mL, 183mmol, 2.5eq.) and DIPEA (1.28mL, 7.32mmol, 0.1eq.), then drip again at 0 DEG C chlorobenzoyl chloride (10.2mL, 87.84mmol, 1.2eq.), gained reactant liquor is stirred at room temperature 16h, then is added thereto to 100mL saturated sodium bicarbonate solution.Separate is organic The most respectively with saturated ammonium chloride solution (60mL) and saturated nacl aqueous solution (60mL) washing, then it is dried with sodium sulphate, finally filters.Filtrate Through being concentrated under reduced pressure to give colorless oil title compound (16.83g, 92%).
1H NMR(400MHz,CD3OD):δ8.06(d,2H),7.56(m,1H),7.45(m,2H),4.36(d,2H),3.18(d,6H),2.52(m, 1H),2.37(m,2H),2.01(m,2H).
Step 5:(3-oxygen cyclobutyl) synthesis of methyl benzoic acid ester
(3,3-bi-methoxy cyclobutane base) methyl benzoic acid ester (20.15g, 80.6mmol, 1eq.) is dissolved in CH3In CN (100mL), and to it Middle addition 1M sulfuric acid (40mL, 40.3mmol, 0.5eq.).Reactant liquor at room temperature reacts about 2h, is then adjusted by reactant liquor with saturated sodium bicarbonate Its pH to 7, then reduced pressure concentration, add 60mL saturated nacl aqueous solution in residue.Gained mixture EtOAc (50mL x 2) extracts, and has Machine washs with saturated nacl aqueous solution (50mL x 2) mutually, and anhydrous sodium sulfate is dried, then reduced pressure concentration, and residue obtained purifying through silica gel column chromatography (is washed De-agent: PE/EtOAc (V/V)=7/1) obtain colorless oil title compound (16.7g, 98%).
1H NMR(400MHz,CD3OD):δ8.02(m,2H),7.57(m,1H),7.45(m,2H),4.49(d,2H),3.26-3.21(m,2H), 3.03-2.97(m,3H).
Step 6:(3-hydroxyl-3-(nitromethyla) cyclobutyl) synthesis of methyl benzoic acid ester
At-10 DEG C, nitromethane (8mL, 89mmol) is slowly added in the THF solution (10mL) of TBAF (3.2g, 42mmol), Stir about 30min, then adds (3-oxygen cyclobutyl) methyl benzoic acid)) THF (5mL) solution of ester (2.8g, 9mmol), reactant liquor is at this At a temperature of be stirred overnight.After completion of the reaction, add saturated ammonium chloride solution (20mL), then extract with EtOAc (20mL x 3), having of merging Machine anhydrous sodium sulfate is dried, then reduced pressure concentration.Residue obtained purifying through silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=5/1) obtains Colorless oil title compound (3.1g, 91%).
MS:(ESI,pos.ion)m/z:266.3[M+1]+.
Step 7:(3-(amino methyl)-3-hydroxycyclobutyl) synthesis of methyl benzoic acid ester
Pd/C (0.1g, 10%) is joined the 15mL methyl alcohol of (3-hydroxyl-3-(nitromethyla) cyclobutyl) methyl benzoic acid ester (3.1g, 45mmol) In solution, with hydrogen balloon as hydrogen source, the most at room temperature react overnight.After having reacted, by reacting liquid filtering, gained filtrate reduced in volume obtains Colorless oil title compound (2.9g, 99%).
MS:(ESI,pos.ion)m/z:236.3[M+1]+.
Intermediate 3:(3-(amino methyl) cyclobutyl) methyl benzoic acid ester
Step 1:(3-(nitro methene) cyclobutyl) synthesis of methyl benzoic acid ester
Triethylamine (11mL, 78.64mmol, 2eq.) and methylsufonyl chloride (3.65mL, 47.18mmol, 1.2eq.) are joined (3-hydroxyl -3-(nitromethyla) cyclobutyl) methyl benzoic acid ester (8.1g, 39.32mmol, 1eq.) DCM (100mL) solution in, reactant liquor be placed in 0 DEG C anti- Answering 5h, be subsequently adding saturated ammonium chloride solution 60mL, the organic phase of separation saturated nacl aqueous solution (50mL x 2) washs, and uses anhydrous slufuric acid Sodium is dried and reduced pressure concentration.It is titled that gained residue silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=5/1) purifying obtains colorless oil Compound (7.5g, 81%).
MS:(ESI,pos.ion)m/z:285.3[M+1]+.
Step 2:(3-(amino methyl) cyclobutyl) synthesis of methyl benzoic acid ester
(3-(nitro methene) cyclobutyl) methyl benzoic acid ester (10g, 35mmol) is dissolved in 100mL methyl alcohol, then add Pd/C (10%, 1.03g), reactant liquor is stir about 2h under the hydrogen of 1 atmospheric pressure and under room temperature, and after completion of the reaction, diatomite is filtrated to get 9.54g crude product, can be straight Connect and react for next step.
MS:(ESI,pos.ion)m/z:285.3[M+1]+.
Embodiment 1:3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formonitrile HCN
The synthesis of step 1:3-chloropyrazine-2-formonitrile HCN
At 0 DEG C, toward 3-HYDROXYPYRAZINE-2-formamide (5g, 37mmol) chlorobenzene (20mL) solution in gradually drip POCl3 (2mL, 40 And diisopropylethylamine (3.5mL, 80mmol) mmol).Reactant liquor is placed in 90 DEG C and is stirred overnight.After completion of the reaction, reduced pressure concentration reactant liquor, Gained residue 20mL water dilutes, and gained mixture EtOAc (20mL x 3) extracts, and the organic phase anhydrous sodium sulfate of merging is dried, After filtration, decompression removes, and residue purifies through silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=10/1) and obtains white solid title compound (4.5g, 79%).
1H NMR(400MHz,CD3OD):δ8.67(d,1H),8.63(d,1H).
Step 2:(3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
Under room temperature, (3-(amino methyl) cyclobutyl) methyl benzoic acid ester (0.3g, 5mmol) and triethylamine (0.2mL, 12mmol) are joined 3- In the THF (10mL) of chloropyrazine-2-formonitrile HCN (0.5g, 6mmol), reactant liquor reacts two hours, after completion of the reaction, reduced pressure concentration, institute at 25 DEG C Obtain residue and obtain white solid title compound (0.32g, 54%) through silica gel column chromatography purifying (eluant, eluent: PE/EtOAc (V/V)=5/1).
1H NMR(400MHz,DMSO-d6):δ8.62(d,1H),8.02(d,1H),7.38-7.82(m,5H),5.42(s,1H),3.63(m,2H), 3.54(m,2H),2.68(m,2H),2.28-2.29(m,1H),2.26-2.28(m,2H),1.68-1.70(m,1H).
The synthesis of step 3:3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formonitrile HCN
(3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.21g, 4mmol) is dissolved in absolute methanol (8mL), then Add the aqueous solution (2mL) of NaOH (0.01g, 5mmol).Reactant mixture is stirred at room temperature about 1h.After having reacted, reactant liquor is concentrated, Gained residue through silica gel chromatography (eluant, eluent: PE/EtOAc (V/V)=5/1) obtain white solid title compound (30mg, 28%).
1H NMR(400MHz,DMSO-d6):δ8.21(d,1H),7.38(d,1H),3.45(m,2H),3.34(m,2H),2.36(m,1H), 2.01-1.99(m,3H),1.77(m,1H),1.69(m,1H).
Embodiment 2:3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
Step 1:3-((3-first amidino-pyrazine-2-base amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
(3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.2g, 3mmol) is dissolved in 10mL methyl alcohol, is subsequently adding NaOMe(0.04g,6mmol).Reactant liquor is stirred at room temperature 1h, adds NH4Cl (0.03g, 5mmol), gained reactant liquor at room temperature continues Continue and be stirred overnight.After having reacted, reduced pressure concentration, the residue obtained purifies through silica gel column chromatography (eluant, eluent: DCM/MeOH=10/1) and obtains white Solid-like title compound (0.12g, 79%).
MS:(ESI,pos.ion)m/z:268.3[M+1]+.
The synthesis of step 2:3-((3-(methylol) cyclobutyl) methylamino) pyrazine-2-carbonamidine
By 3-((3-first amidino-pyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.25g, 4mmol) is dissolved in absolute methanol (8mL), Adding the aqueous solution (2mL) of NaOH (0.01g, 5mmol), reactant mixture is stirred at room temperature about 1h.After having reacted, reactant liquor is subtracted Pressure concentrate, residue obtained through silica gel column chromatography purify (eluant, eluent: DCM/MeOH (V/V)=7/1) obtain white solid title compound (40 Mg, 48%).
1H NMR(400 MHz,DMSO-d6):δ9.04(s,2H),8.21(s,1H),7.88(s,1H),7.79(s,1H),4.42(s,1H), 3.29-3.34(m,4H),2.38(m,1H),2.23(m,1H),2.17-2.19(m,2H),2.01-1.99(m,2H).
Embodiment 3:3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
Step 1:(3-((3-formamido pyrazine-2-base amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
Potassium carbonate (3.8mg, 0.02mmol, 0.1eq) is joined (3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid (0.12 G, 2mmol, 1eq) DMSO solution in (10mL), mixture stirring 30min, add 10mL hydrogen peroxide, gained mixture continues normal temperature Adding 5mL shrend after reaction 2h to go out reaction, solids removed by filtration product, filtrate reduced in volume obtains residue, is directly used in next step and reacts.
MS:(ESI,pos.ion)m/z:268.3[M+1]+.
The synthesis of step 2:3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
(3-((3-formamido pyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.05g, 0.8mmol) is dissolved in absolute methanol (8mL) In, adding water (2mL) solution of NaOH (0.03g, 1.2mmol), mixture is stirred at room temperature about 6h.After having reacted, by reactant liquor Reduced pressure concentration, residue obtained through silica gel column chromatography purify (eluant, eluent: DCM/MeOH (V/V)=7/1) obtain white solid title compound (30 Mg, 57%).
MS:(ESI,pos.ion)m/z:253.2[M+1]+；
1H NMR(400 MHz,DMSO-d6):δ8.21(d,1H),7.38(d,1H),3.45(m,2H),3.34(m,2H),2.36(m,1H), 2.01-1.99(m,3H),1.77(m,1H),1.69(m,1H).
Embodiment 4:N-hydroxyl-3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
Step 1:(3-(((3-(N-hydroxy formamidine base) pyrazine-2-base) amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
(3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.2g, 3mmol) is dissolved in 10mL methyl alcohol, adds methyl alcohol Sodium (0.04g, 6mmol).Reactant mixture is stirred at room temperature 1h, is subsequently adding NH2OH (0.18g, 5mmol), gained mixture is in room temperature Lower reaction is overnight.After completion of the reaction, by reactant liquor reduced pressure concentration, residue by silicagel column chromatography (eluant, eluent: DCM/MeOH (V/V)=10/1) is pure Change and obtain white solid title compound (0.12g, 79%).
MS:(ESI,pos.ion)m/z:268.2[M+1]+.
The synthesis of step 2:N-hydroxyl-3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
Molten at the 10mL methyl alcohol of (3-(((3-(N-hydroxy formamidine base) pyrazine-2-base) amino) methyl) cyclobutyl) methyl benzoic acid ester (0.08g, 1mmol) In liquid, adding NaOH (0.02g, 2mmol), then reaction system is stirred at room temperature 1h.After having reacted, by reactant liquor reduced pressure concentration, Residue by silicagel column chromatography (eluant, eluent: DCM/MeOH (V/V)=7/1) purifies and obtains white solid title compound (7mg, 32%).
MS:(ESI,pos.ion)m/z:253.2[M+1]+；
1H NMR(400MHz,DMSO-d6):δ8.21(d,1H),7.38(d,1H),3.45(m,2H),3.34(m,2H),2.36(m,1H), 2.01-1.99(m,3H),1.77(m,1H),1.69(m,1H).
The fluoro-3-of embodiment 5:6-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
Step 1:(3-(((3-cyano group-5-Calmazine-2-base) amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
Under room temperature, by 3,6-bis-Calmazine-2-formamide (1.0g, 70mmol, 1eq.) is dissolved in THF (10mL), adds (3-(amino methyl) Cyclobutyl) methyl benzoic acid ester (2.16g, 92mmol, 1.3eq.) and triethylamine (1.8g, 182mmol, 2.6eq.), reactant liquor reacts 2 at 25 DEG C Hour.After having reacted, in reactant mixture, add saturated nacl aqueous solution (30mL), extract with EtOAc (30mL x 2), merging organic Washing (30mLx2) with saturated nacl aqueous solution, be dried with anhydrous sodium sulfate, removal of solvent under reduced pressure obtains crude product, and crude product is through silica gel column chromatography Purify (eluant, eluent: PE/EtOAc (V/V)=5/1) and obtain white solid title compound (1.8g, 85%).
MS:(ESI,pos.ion)m/z:341.3[M+1]+.
Step 2:(3-(((3-formamido-5-Calmazine-2-base) amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
Potassium carbonate (3.8mg, 0.02mmol, 0.1eq) is joined (3-(((3-cyano group-5-Calmazine-2-base) amino) methyl) cyclobutyl) methyl benzoic acid In DMSO (10mL) solution of ester (100mg, 0.28mmol, 1eq.), reactant liquor is stirred at room temperature and reacts about 30min, adds 10mL dioxygen Water, gained mixture continues to add after insulation reaction 2h 5mL shrend and goes out reaction, solids removed by filtration product, the filtrate residue through being concentrated under reduced pressure to give It is directly used in next step reaction.
The synthesis of the fluoro-3-of step 3:6-(((3-(hydroxymethyl) cyclobutyl) methyl) amino) pyrazine-2-formamide
By raw material (3-(((3-formamido-5-Calmazine-2-base) amino) methyl) cyclobutyl) methyl benzoic acid ester (200mg, 0.27mmol, 1.0eq.) Being dissolved in absolute methanol (8mL), add the aqueous solution (2mL) of NaOH (11.17mg, 0.27mmol, 1.0eq.), mixture is at room temperature Stir about 3h.After having reacted, being concentrated by reactant liquor, it is pure that gained residue separates (eluant, eluent: PE/EtOAc (V/V)=1/1) through silica gel column chromatography Change and obtain white solid title compound (108mg, 59%).
MS:(ESI,pos.ion)m/z:255.3[M+1]+；
1H NMR(400MHz,CD3OD):δ8.16-8.13(dd,1H),3.56-3.53(m,2H),3.46-3.44(m,2H),2.56-2.50(m, 2H),2.48-2.46(m,1H),2.17-2.13(m,2H),2.10-1.96(m,1H).
Embodiment 6:6-fluoro-N-hydroxyl-3-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
Potassium carbonate (3.8mg, 0.02mmol, 0.1eq) is joined (3-(((3-cyano group-5-Calmazine-2-base) amino) methyl) cyclobutyl) methyl benzoic acid In MeOH (10mL) solution of ester (100mg, 0.28mmol, 1eq.), reactant liquor is stirred at room temperature about 30min, is subsequently adding NH2OH (0.1mL, 1.2mmol), in reaction system, gained reactant mixture at room temperature continues to stir 2h.After completion of the reaction, add 20mL water, mixed Compound EtOAc (20mL x 2) extracts, and organic phase saturated nacl aqueous solution (15mL) washs, and the organic phase of merging anhydrous sodium sulfate is done Dry, then reduced pressure concentration, it is titled that gained residue obtains white solid through silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=1/1) purifying Compound (57mg, 75%).
MS:(ESI,pos.ion)m/z:270.3[M+1]+；
1H NMR(400MHz,DMSO-d6):δ7.86(dd,1H),3.56-3.44(m,2H),3.42-3.30(m,2H),2.20-2.23(m,2H), 2.20-2.18(m,2H),1.79-1.76(m,2H).
The fluoro-3-of embodiment 7:6-(((3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
(3-(((3-cyano group-5-Calmazine-2-base) amino) methyl) cyclobutyl) methyl benzoic acid ester (80mg, 0.24mmol, 1eq.) is dissolved in MeOH (3mL), in, methyl alcohol (1mL) solution of sodium methoxide (24mg, 0.44mmol, 1.8eq.) is added.Mixture is stirred at room temperature 3.5h, then Adding ammonium chloride (29mg, 0.54mmol, 2.2eq.), gained mixture continues to be stirred at room temperature 12h.After having reacted, reduced pressure concentration, residue Purifying through silica gel column chromatography (eluant, eluent DCM/MeOH (V/V)=10/1) and obtain 50mg crude product, this crude product purifies through preparation HPLC and obtains white admittedly Body shape title compound (7mg, 11%).
MS:(ESI,pos.ion)m/z:254.1[M+1]+；
1H NMR(400MHz,CD3OD):δ8.00-7.98(dd,1H),3.56-3.44(m,2H),3.30-3.29(m,2H),2.56-2.50(m, 2H),2.48-2.47(m,1H),2.37-2.33(m,2H),2.11-2.09(m,1H).
Embodiment 8:3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formonitrile HCN
Step 1:(3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) synthesis of methyl benzoic acid ester
Under room temperature, 3-chloropyrazine-2-formonitrile HCN (0.5g, 6mmol) is dissolved in THF (10mL), adds (3-(amino methyl)-3-hydroxycyclobutyl) first Yl benzoic acid ester (0.3g, 5mmol) and triethylamine (0.2mL, 12mmol), reactant mixture reacts two hours at 25 DEG C, after having reacted, decompression Concentrating, residue purifies (eluant, eluent: PE/EtOAc (V/V)=5/1) through silica gel column chromatography and obtains white solid target compound (0.32g, 54%).
MS:(ESI,pos.ion)m/z:339.4[M+1]+.
The synthesis of step 2:3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formonitrile HCN
Raw material (3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.32g, 5.3mmol) is dissolved in absolute methanol (10mL) In, adding water (2mL) solution of NaOH (0.41g, 10mmol), mixture is stirred at room temperature about 1h.After having reacted, reduced pressure concentration, Residue obtained obtain white solid target compound (120mg, 46%) through silica gel chromatography (eluant, eluent: PE/EtOAc (V/V)=4/1).
MS:(ESI,pos.ion)m/z:235.3[M+1]+；
1H NMR(400MHz,CD3OD):δ8.17(d,1H),7.90(d,1H),5.84(s,1H),3.69(d,2H),3.67(s,2H),2.62(m, 1H),2.28(m,2H),2.17(m,2H).
Embodiment 9:N-hydroxyl-3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
Potassium carbonate (3.8mg, 0.02mmol, 0.1eq) is joined (3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester In MeOH (10mL) solution of (100mg, 0.28mmol, 1eq.), reactant liquor is stirred at room temperature about 30min.It is subsequently adding NH2OH(0.1mL, 1.2mmol) in reaction system, reactant liquor at room temperature continues to react 2h.After completion of the reaction, 20mL water is added, with EtOAc (20mL x 2) Extraction, organic phase saturated nacl aqueous solution (15mL) washs, and anhydrous sodium sulfate is dried, reduced pressure concentration, and gained residue is pure through silica gel column chromatography Change (eluant, eluent: DCM/MeOH (V/V)=7/1) and obtain white solid target compound (0.03g, 57%).
MS:(ESI,pos.ion)m/z:268.3[M+1]+；
1H NMR(400MHz,DMSO-d6):δ8.21(d,1H),7.38(d,1H),3.45(m,2H),3.34(m,2H),2.36(m,1H), 2.01-1.99(m,3H),1.77(m,1H),1.69(m,1H).
Embodiment 10:3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
The synthesis of step 1:3-((3-(benzyloxymethyl)-1-hydroxycyclobutyl) methylamino) pyrazine-2-carbonamidine
Raw material (3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.2g, 1.4mmol) is dissolved in 10mL methyl alcohol, then Add NaOMe (0.02g, 3mmol).Mixture is stirred at room temperature 1h, adds NH4Cl (0.03g, 5mmol), gained reactant mixture continues Continue and at room temperature react overnight.After having reacted, by reactant liquor reduced pressure concentration, residue obtained through silica gel column chromatography (eluant, eluent: DCM/MeOH (V/V) =10/1) purifying obtains white solid title compound (0.12g, 68%).
MS:(ESI,pos.ion)m/z:356.3[M+1]+.
The synthesis of step 2:3-((1-hydroxyl-3-(methylol) cyclobutyl) methylamino) pyrazine-2-carbonamidine
3-((3-(benzyloxymethyl)-1-hydroxycyclobutyl) methylamino) pyrazine-2-carbonamidine (0.12g, 1mmol) is dissolved in absolute methanol (8mL) In, adding water (2mL) solution of NaOH (0.08g, 2mmol), reactant mixture is stirred at room temperature about 6h, after having reacted, will reaction Reduced pressure concentration, gained residue purifies (eluant, eluent: DCM/MeOH (V/V)=10/1) through silica gel column chromatography and obtains titled compound as white solid (30mg, 39%).
MS:(ESI,pos.ion)m/z:252.1[M+1]+；
1H NMR(400MHz,DMSO-d6):δ8.21(d,1H),7.38(d,1H),3.45(m,2H),3.34(m,2H),2.36(m,1H), 2.01-1.99(m,3H),1.77(m,1H),1.69(m,1H).
Embodiment 11:3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
Step 1:(3-((3-formamido pyrazine-2-base amino) methyl)-3-hydroxycyclobutyl) synthesis of methyl benzoic acid ester
Potassium carbonate (100mg, 4mmol, 0.1eq) is joined (3-((3-cyanopyrazine-2-base amino) methyl) cyclobutyl) methyl benzoic acid ester (0.32g, 5.3mmol, 1eq) DMSO (10mL) solution in, mixture is stirred at room temperature about 30min, adds hydrogen peroxide (0.2mL, 6mmol), institute Mixture continues after insulated and stirred 1h, add 10mL shrend and go out reaction, solids removed by filtration product, by filtrate reduced in volume, residue is through silica gel Column chromatography purifies (eluant, eluent: PE/EtOAc (V/V)=2/1) and obtains white solid title compound (0.21g, 52%).
MS:(ESI,pos.ion)m/z:357.3[M+1]+。
The synthesis of step 2:3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
(3-((3-formamido pyrazine-2-base amino) methyl)-3-hydroxycyclobutyl) methyl benzoic acid ester (0.21g, 2.7mmol) is dissolved in absolute methanol (10mL) in, adding water (2mL) solution of NaOH (0.08g, 2mmol), mixture is stirred at room temperature about 6h, after having reacted, and will Reactant mixture concentrates, and gained residue purifies (eluant, eluent: DCM/MeOH (V/V)=7/1) through silica gel column chromatography and obtains white solid title Compound (50mg, 51%).
MS:(ESI,pos.ion)m/z:253.3[M+1]+；
1H NMR(400MHz,DMSO-d6):δ8.21(d,1H),7.38(d,1H),3.45(m,2H),3.34(m,2H),2.36(m,1H), 2.01-1.99(m,3H),1.77(m,1H),1.69(m,1H).
The fluoro-3-of embodiment 12:6-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
Step 1:(3-(((3-cyano group-5-Calmazine-2-base) amino) methyl)-3-hydroxycyclobutyl) synthesis of methyl benzoic acid ester
By 3,6-bis-Calmazine-2-formamide (1.0g, 70mmol, 1eq.), (3-(amino methyl)-3-hydroxycyclobutyl) methyl benzoic acid ester (2.16g, 92 Mmol, 1.3eq.) and Et3N (1.8g, 182mmol, 2.6eq.) join in the THF of 20mL, reactant mixture stirs 2 hours at 25 DEG C, After having reacted, adding 20mL saturated nacl aqueous solution, gained mixture EtOAc (20mL x 2) extracts, the organic phase saturated common salt of merging Water 20mL washs, and is dried with anhydrous sodium sulfate, then reduced pressure concentration, and residue is pure through silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=5/1) Change and obtain white solid title compound (1.8g, 85%).
MS:(ESI,pos.ion)m/z:357.3[M+1]+.
Step 2:(3-(((3-formamido-5-Calmazine-2-base) amino) methyl)-3-hydroxycyclobutyl) synthesis of methyl benzoic acid ester
Potassium carbonate (3.8mg, 0.02mmol, 0.1eq) is joined (3-(((3-cyano group-5-Calmazine-2-base) amino) methyl)-3-hydroxycyclobutyl) methyl In DMSO (10mL) solution of benzoic ether (100mg, 0.28mmol, 1eq.), mixture is stirred at room temperature about 30min, adds 30% H2O2(0.1mL, 1.2mmol), gained mixture at room temperature continues to stir 2h.After having reacted, add 20mL water, be filtrated to get white solid Title compound (83mg, 80%).
MS:(ESI,pos.ion)m/z:375.3[M+1]+.
The synthesis of the fluoro-3-of step 3:6-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
By (3-(((3-formamido-5-Calmazine-2-base) amino) methyl)-3-hydroxycyclobutyl) methyl benzoic acid ester (200mg, 0.27mmol, 1.0eq.) Being dissolved in absolute methanol (8mL), add water (2mL) solution of NaOH (11.17mg, 0.27mmol, 1.0eq.), reactant liquor is in room temperature Lower stirring 3h.After having reacted, by reactant liquor reduced pressure concentration, residue purifies through silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=1/1) To titled compound as white solid (108mg, 59%).
MS:(ESI,pos.ion)m/z:271.1[M+1]+；
1H NMR(400MHz,CD3OD):δ8.12-8.09(dd 1H),3.64(s,2H),3.53-3.52(d,2H),2.23-2.18(m,2H), 2.08-2.06(m,1H),62.04-61.74(m,2H).
Embodiment 13:6-fluoro-N-hydroxyl-3-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-formamide
Potassium carbonate (3.8mg, 0.02mmol, 0.1eq) is joined (3-(((3-cyano group-5-Calmazine-2-base) amino) methyl)-3-hydroxycyclobutyl) first In MeOH (10mL) solution of yl benzoic acid ester (100mg, 0.28mmol, 1eq.), reactant liquor is stirred at room temperature about 30min.Add NH2OH (0.1mL, 1.2mmol), in reaction system, continues stirring 2h under gained mixture room temperature again.After having reacted, add 20mL water, Extracting with EtOAc (20mL x 2), organic phase saturated nacl aqueous solution (15mL) washs, then is dried with sodium sulphate, then reduced pressure concentration. Gained residue purifies through silica gel column chromatography (eluant, eluent: PE/EtOAc (V/V)=1/1) and obtains titled compound as white solid (69mg, 87%).
MS:(ESI,pos.ion)m/z:286.1[M+1]+；
1H NMR(400MHz,DMSO-d6):δ7.89-7.86(dd,1H),3.64(s,2H),3.53-3.52(m,2H),2.23-2.18(m,2H), 2.08-2.04(m,1H),1.79-1.77(m,2H).
The fluoro-3-of embodiment 14:6-(((1-hydroxyl-3-(methylol) cyclobutyl) methyl) amino) pyrazine-2-carbonamidine
By 3-(((3-cyano group-5-Calmazine-2-base) amino) methyl)-3-hydroxycyclobutyl) methyl benzoic acid ester (80mg, 0.24mmol, 1eq.) is dissolved in In MeOH (3mL), add methyl alcohol (1mL) solution of sodium methoxide (24mg, 0.44mmol, 1.8eq.).Mixture is stirred at room temperature 3.5 H. adding ammonium chloride (29mg, 0.54mmol, 2.2eq.), gained mixture continues to be stirred at room temperature 12h.After having reacted, reduced pressure concentration, residual Slag purifies through silica gel column chromatography (eluant, eluent: DCM/MeOH (V/V)=10/1), then obtains titled compound as white solid through preparation HPLC purifying (13mg, 20%).
MS:(ESI,pos.ion)m/z:270.1[M+1]+；
1H NMR(400MHz,CD3OD):δ8.00-7.98(dd,1H),5.04(br,6H),3.56-3.44(m,2H),3.30-3.29(m,2H), 2.56-2.50(m,2H),2.48-2.47(m,1H),2.37-2.33(m,2H).
Anti-influenza virus activity measures
96 orifice plate CPE determination method steps:
Spread 96 orifice plates, 5000 mdck cells of every hole inoculation, 37 DEG C, 5%CO2, incubated overnight.
By DMSO and serum free medium diluted compounds to suitable concn, DMSO final concentration of 1%.
-80 DEG C of frozen influenza virus H1N1 (A/weiss/43) are diluted with culture medium.
Every hole adds the compound after 50 μ L dilutions and the virus liquid (final MOI=0.01) after 50 μ L dilution.
96 orifice plates are placed in 37 DEG C, 5%CO2, cultivate 3 days.
Every hole adds 20 μ L MTT, is placed in 37 DEG C, hatches 4 hours.
Measure the first a ceremonial jade-ladle, used in libation amount that MTT is generated by living cells reduction, and the CPE calculating influenza virus mediation accordingly is detected compound suppression Percentage.
96 orifice plates mensuration cytotoxicity steps:
Spread 96 orifice plates, 5000 mdck cells of every hole inoculation, 37 DEG C, 5%CO2, incubated overnight.
By DMSO and serum free medium diluted compounds to suitable concn, DMSO final concentration of 1%.
Every hole adds the compound after 50 μ L dilutions and 50 μ L nutrient solutions.
96 orifice plates are placed in 37 DEG C, 5%CO2, cultivate 3 days.
Every hole adds 20 μ L MTT, is placed in 37 DEG C, 4 hours.
Measure the first a ceremonial jade-ladle, used in libation amount that MTT is generated by living cells reduction, and calculate the cytotoxicity of test compound accordingly.
Data analysis: % inhibiting rate=(ODT–ODV)/(ODC–ODV) × 100%
% cytotoxicity=ODT/ODC× 100%
% activity=% inhibiting rate-% cytotoxicity
ODT,ODV, and ODCThe absorptivity of representative test compound respectively, virus-infected controls (without compound ,+1%DMSO), with And cell blank comparison (virus-free, without compound ,+1%DMSO)
OD value=OD570–OD630(MTT)
Table one: part of compounds infected by influenza H1N1 (A/weiss/43) experiment in vitro activity value of the present invention
As seen from the above table, compound involved in the present invention has obvious anti-influenza virus activity.
It will be apparent to one skilled in the art that present invention is not limited to foregoing illustrative embodiment, and other tool can be embodied in Without departing from its essential characteristics in bodily form formula.It is therefore contemplated that each embodiment is the most all considered illustrative and nonrestrictive, Ying Can According to appended claims rather than these embodiments aforementioned, therefore, all changes in the implication and scope of appended claims equivalents Change and be included in herein.
In the description of this specification, reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. Description means to combine this embodiment or example describes specific features, structure, material or feature be contained in the present invention at least one embodiment or In example.In this manual, the schematic representation to above-mentioned term is not necessarily referring to identical embodiment or example.And, description concrete Feature, structure, material or feature can combine in any one or more embodiments or example in an appropriate manner.
Although above it has been shown and described that embodiments of the invention, it is to be understood that above-described embodiment is exemplary, it is impossible to it is right to be interpreted as The restriction of the present invention, those of ordinary skill in the art in the case of without departing from the principle of the present invention and objective within the scope of the invention can on Stating embodiment to be changed, revise, replace and modification, the scope of the present invention is limited by claim and equivalent thereof.

Claims (7)

1. a compound, it is the compound as shown in formula (I), or its stereoisomer, geometric isomer, dynamic isomer or pharmaceutically can connect The salt being subject to,
Wherein, R1For hydroxyl, C1-3Hydroxy alkyl, C1-3Alkyl, C1-3Alkoxyl, amino or C1-3Alkylamino；
R2For-CN ,-C (=NH) NH2,-C (=O) NH2,-C (=S) NH2,-C (=NH) NHOH ,-C (=NH) NH-C1-3Alkyl or-C (=O) O-C1-3 Alkyl；
R3For hydrogen or C1-3Alkyl；
R4For hydrogen, hydroxyl, amino, C1-3Alkylamino, C1-3Alkoxyl, C1-3Alkyl, F, Cl, Br or I；With
R5For hydrogen, hydroxyl, C1-3Alkyl or C1-3Hydroxy alkyl.
2. compound as claimed in claim 1, wherein
R1For hydroxyl, hydroxymethyl, hydroxyethyl, 2-hydroxypropyl, methyl, ethyl, n-pro-pyl, isopropyl, methoxyl group, ethyoxyl, ammonia Base, N-methylamino, N-ethylamino or N, N-dimethylamino；
R2For-CN ,-C (=NH) NH2,-C (=O) NH2,-C (=S) NH2,-C (=NH) NHOH ,-C (=NH) NHCH3、 -C (=NH) NHCH2CH3,-C (=O) OCH3Or-C (=O) OCH2CH3；With
R3For hydrogen, methyl or ethyl；
R4For hydrogen, hydroxyl, amino, N-methylamino, N-ethylamino, N, N-dimethylamino, methoxyl group, ethyoxyl, methyl, ethyl, F, Cl, Br or I；With
R5For hydrogen, hydroxyl, methyl, ethyl, n-pro-pyl, isopropyl, hydroxymethyl, hydroxyethyl or 2-hydroxypropyl.
3., according to the compound described in any one of claim 1-2, it is the structure of one of or its stereoisomer, geometric isomer, mutually Tautomeric or pharmaceutically acceptable salt,
4. a pharmaceutical composition, comprises the arbitrary described compound of claim 1-3, and pharmaceutically acceptable assistant agent.
Pharmaceutical composition the most according to claim 4, wherein said assistant agent is carrier, excipient, diluent or its any combination.
Pharmaceutical composition the most according to claim 4, it further comprises anti-influenza virus medicament, wherein said anti-influenza virus medicament For Peramivir, zanamivir, Oseltamivir, that Ni Na meter Wei, Favipiravir, amantadine, Rimantadine, arbidol, Ribavirin, Si Tafulin, ingavirin (Ingavirin) or a combination thereof.
7. the arbitrary described compound of claim 1-3 or the arbitrary described pharmaceutical composition of claim 4-6 prevent in preparation, treat or alleviate Purposes in patient's influenza virus medicine.
CN201410667916.2A 2014-11-20 2014-11-20 Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes Active CN104402833B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410667916.2A CN104402833B (en) 2014-11-20 2014-11-20 Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410667916.2A CN104402833B (en) 2014-11-20 2014-11-20 Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes

Publications (2)

Publication Number Publication Date
CN104402833A CN104402833A (en) 2015-03-11
CN104402833B true CN104402833B (en) 2016-08-24

Family

ID=52640513

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410667916.2A Active CN104402833B (en) 2014-11-20 2014-11-20 Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes

Country Status (1)

Country Link
CN (1) CN104402833B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109953977A (en) * 2017-12-25 2019-07-02 深圳市华力康生物医药有限公司 A kind of nasal inhalation powder formulation and its device
CN114644597B (en) * 2022-03-23 2023-11-21 南京农业大学 Tetra-substituted pyrazine derivative and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1942437A (en) * 2004-02-16 2007-04-04 埃斯特韦实验室有限公司 Substituted azetidine compounds as cyclooxygenase-1-cyclooxygenase-2 inhibitors, and their preparation and use as medicaments
CN101268064A (en) * 2005-07-29 2008-09-17 奥米罗实验室有限公司 Pyrazine derivatives useful as adenosine receptor antagonists
CN101610772A (en) * 2007-02-16 2009-12-23 富山化学工业株式会社 The method that comprises pharmaceutical composition with the drug combination that uses pyrazines derivatives of pyrazines derivatives

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012082817A1 (en) * 2010-12-16 2012-06-21 Boehringer Ingelheim International Gmbh Biarylamide inhibitors of leukotriene production

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1942437A (en) * 2004-02-16 2007-04-04 埃斯特韦实验室有限公司 Substituted azetidine compounds as cyclooxygenase-1-cyclooxygenase-2 inhibitors, and their preparation and use as medicaments
CN101268064A (en) * 2005-07-29 2008-09-17 奥米罗实验室有限公司 Pyrazine derivatives useful as adenosine receptor antagonists
CN101610772A (en) * 2007-02-16 2009-12-23 富山化学工业株式会社 The method that comprises pharmaceutical composition with the drug combination that uses pyrazines derivatives of pyrazines derivatives

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Discovery of LAS101057: A Potent, Selective, and Orally Efficacious A2B Adenosine Receptor Antagonist;Paul Eastwood，et al.;《ACS Medicinal Chemistry Letters》;20111231(第2期);第213-218页 *

Also Published As

Publication number Publication date
CN104402833A (en) 2015-03-11

Similar Documents

Publication Publication Date Title
WO2021078312A1 (en) Cycloalkyl and hetero-cycloalkyl inhibitors, preparation methods therefor, and use thereof
CN106916177B (en) A kind of deuterated dipeptide boronic acid or its ester type compound and its synthetic method and purposes
CN106632383B (en) Curcuma zedoary 01 derivatives and preparation method thereof and the application in antitumor drug
CN105884779B (en) Application as the compound of hepatitis c inhibitor and its in drug
CN105968101B (en) Application as the compound of hepatitis c inhibitor and its in drug
JP2022549866A (en) 2H-benzopyran derivatives as CRAC inhibitors
CN104402833B (en) Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes
CN108341752A (en) The aminated compounds for inhibiting SSAO/VAP-1 and its application in medicine
CN109956975A (en) Liver delivers Entecavir pro-drug nucleosides cyclic phosphate compound and application
CN104447583B (en) Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes
EP3851108A1 (en) Borate-based drug and use thereof
AU2021250904B2 (en) Pyridinethiones, pharmaceutical compositions thereof, and their therapeutic use for treating a proliferative, inflammatory, neurodegenerative, or immune-mediated disease
CN104387288B (en) As the compound of neuraminidase inhibitor and the application in medicine thereof
CN104496918B (en) Pyrazines derivatives and application thereof
CN110981865B (en) Medicine for treating brain glioma and preparation method thereof
WO2021189017A1 (en) Stable polymorphic compositions of brequinar sodium and methods of use and manufacture thereof
CN104447584B (en) Pyrazine compounds containing cyclobutane substituent and combinations thereof thing and purposes
WO2021062168A1 (en) Synthetic sphingolipid inspired molecules with heteroaromatic appendages, methods of their synthesis and methods of treatment
CN104447585B (en) Pyrazine compounds and combinations thereof thing and purposes
CN104402754B (en) As the compound of neuraminidase inhibitor and the application in medicine thereof
JP2022540360A (en) Quinoline and cinnoline derivatives as DNA-PK inhibitors
WO2015195474A1 (en) Hydroxypyridone derivatives, pharmaceutical compositions thereof, and their therapeutic use for treating inflammatory, neurodegenerative, or immune-mediated diseases
CN104529833B (en) Cyclobutane-carboxylic acid compounds replaced and uses thereof
CN110092799B (en) Cyclic compound, preparation method and application thereof
CN104496857B (en) As the compound of neuraminidase inhibitor and the application in medicine thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: Pyrazine compound containing diethylene substituent and composition and application thereof

Effective date of registration: 20190920

Granted publication date: 20160824

Pledgee: Shenzhen branch of China Import and Export Bank

Pledgor: SUNSHINE LAKE PHARMA Co.,Ltd.

Registration number: Y2019990000260

PE01 Entry into force of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right

Date of cancellation: 20211105

Granted publication date: 20160824

Pledgee: Shenzhen branch of China Import and Export Bank

Pledgor: SUNSHINE LAKE PHARMA Co.,Ltd.

Registration number: Y2019990000260

CP03 Change of name, title or address
CP03 Change of name, title or address

Address after: 523808 No.1, Gongye North Road, Songshanhu Park, Dongguan City, Guangdong Province

Patentee after: Guangdong Dongyangguang Pharmaceutical Co.,Ltd.

Address before: 523808 No. 1 Industrial North Road, Songshan Industrial Park, Songshan, Guangdong, Dongguan, Hubei

Patentee before: SUNSHINE LAKE PHARMA Co.,Ltd.

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20240125

Address after: 300000 Tianjin Binhai New Area Free Trade Pilot Zone (Dongjiang Bonded Port Area), No. 6975 Asia Road, Financial and Trade Center South Zone 1-1-2019

Patentee after: Haitong Hengxin International Finance Leasing (Tianjin) Co.,Ltd.

Country or region after: China

Address before: 523808 No.1, Gongye North Road, Songshanhu Park, Dongguan City, Guangdong Province

Patentee before: Guangdong Dongyangguang Pharmaceutical Co.,Ltd.

Country or region before: China