CN104396741A - 风兰专用培养基 - Google Patents
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- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000003448 gibberellin Substances 0.000 claims abstract description 7
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- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 7
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims abstract description 7
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- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims abstract description 7
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940023877 zeatin Drugs 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 10
- RZCJYMOBWVJQGV-UHFFFAOYSA-N 2-naphthyloxyacetic acid Chemical compound C1=CC=CC2=CC(OCC(=O)O)=CC=C21 RZCJYMOBWVJQGV-UHFFFAOYSA-N 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000020197 coconut milk Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 239000000413 hydrolysate Substances 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 6
- 229910000358 iron sulfate Inorganic materials 0.000 claims description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 6
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- 229910052799 carbon Inorganic materials 0.000 claims description 3
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- 102000009027 Albumins Human genes 0.000 abstract 1
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- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 abstract 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 abstract 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 1
- 235000020415 coconut juice Nutrition 0.000 abstract 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 239000003617 indole-3-acetic acid Substances 0.000 abstract 1
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 abstract 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 abstract 1
- 235000009973 maize Nutrition 0.000 abstract 1
- DGZOAOQSNXPLNQ-UHFFFAOYSA-N n-(3-methylbut-3-enyl)-7h-purin-2-amine Chemical compound CC(=C)CCNC1=NC=C2NC=NC2=N1 DGZOAOQSNXPLNQ-UHFFFAOYSA-N 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000003205 fragrance Substances 0.000 description 2
- 241001314540 Neofinetia falcata Species 0.000 description 1
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- 235000008935 nutritious Nutrition 0.000 description 1
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Abstract
本发明公开了风兰专用培养基，由按照重量份计的以下物质制备得到：B5培养基70-80份、硫酸铁15-20份、EDTA二钠8-10份、蔗糖6-8份、葡萄糖6-8份、水解乳蛋白8-10份、玉米胚乳8-12份、西红柿汁6-12份、椰子汁3-12份、吲哚乙酸3-5份、吲哚丁酸3-6份、萘氧乙酸3-6份、ABT生根粉1-5份、玉米素1-4份、激动素1-4份、异戊烯氨基嘌呤1-3份、赤霉素1-3份、琼脂20-30份、活性炭5-15份、水40-50份。本发明的有益效果为：本发明提供的风兰专用培养基，营养丰富，针对风兰植物特性制备，能够有效提高其成活率，市场前景良好。
Description
技术领域
本发明涉及风兰专用培养基。
背景技术
风兰（拉丁学名：Neofinetia falcata (Thunb. ex A. Murray) H. H. Hu）兰科，风兰属附生草本植物。风兰叶短，一般长8～10mm，厚实，有锯齿。一般在春、秋季生长，叶互生，有规则，呈左右互生状。一年里长出两片叶子，一般看叶子的指环就可以猜出风兰的年龄。一般在六、七月份开花，多为乳白色花朵，带有一股浓浓的幽香。一般根茎的生长状态良好时开花。有的品种带有幽香的甜味。其对生长环境要求较高，培育成活困难，难以适应市场需求。
发明内容
本发明的目的就是针对上述现有技术中的缺陷，提供了风兰专用培养基。
为了实现上述目的，本发明提供的技术方案为：风兰专用培养基，由按照重量份计的以下物质制备得到：B5培养基70-80份、硫酸铁15-20份、EDTA二钠8-10份、蔗糖6-8份、葡萄糖6-8份、水解乳蛋白8-10份、玉米胚乳8-12份、西红柿汁6-12份、椰子汁3-12份、吲哚乙酸3-5份、吲哚丁酸3-6份、萘氧乙酸3-6份、ABT生根粉1-5份、玉米素1-4份、激动素1-4份、异戊烯氨基嘌呤1-3份、赤霉素1-3份、琼脂20-30份、活性炭5-15份、水40-50份。
进一步的，上述的风兰专用培养基，其特征在于，由按照重量份计的以下物质制备得到：B5培养基75份、硫酸铁18份、EDTA二钠9份、蔗糖7份、葡萄糖7份、水解乳蛋白9份、玉米胚乳10份、西红柿汁9份、椰子汁8份、吲哚乙酸4份、吲哚丁酸5份、萘氧乙酸5份、ABT生根粉3份、玉米素2份、激动素2份、异戊烯氨基嘌呤2份、赤霉素2份、琼脂25份、活性炭10份、水45份。
本发明的有益效果为：本发明提供的风兰专用培养基，营养丰富，针对风兰植物特性制备，能够有效提高其成活率，市场前景良好。
具体实施方式
实施例1：
风兰专用培养基，由按照重量份计的以下物质制备得到：B5培养基70-80份、硫酸铁15-20份、EDTA二钠8-10份、蔗糖6-8份、葡萄糖6-8份、水解乳蛋白8-10份、玉米胚乳8-12份、西红柿汁6-12份、椰子汁3-12份、吲哚乙酸3-5份、吲哚丁酸3-6份、萘氧乙酸3-6份、ABT生根粉1-5份、玉米素1-4份、激动素1-4份、异戊烯氨基嘌呤1-3份、赤霉素1-3份、琼脂20-30份、活性炭5-15份、水40-50份。
实施例2：
风兰专用培养基，其特征在于，由按照重量份计的以下物质制备得到：B5培养基75份、硫酸铁18份、EDTA二钠9份、蔗糖7份、葡萄糖7份、水解乳蛋白9份、玉米胚乳10份、西红柿汁9份、椰子汁8份、吲哚乙酸4份、吲哚丁酸5份、萘氧乙酸5份、ABT生根粉3份、玉米素2份、激动素2份、异戊烯氨基嘌呤2份、赤霉素2份、琼脂25份、活性炭10份、水45份。
最后应说明的是：以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (2)
1.风兰专用培养基，其特征在于，由按照重量份计的以下物质制备得到：B5培养基70-80份、硫酸铁15-20份、EDTA二钠8-10份、蔗糖6-8份、葡萄糖6-8份、水解乳蛋白8-10份、玉米胚乳8-12份、西红柿汁6-12份、椰子汁3-12份、吲哚乙酸3-5份、吲哚丁酸3-6份、萘氧乙酸3-6份、ABT生根粉1-5份、玉米素1-4份、激动素1-4份、异戊烯氨基嘌呤1-3份、赤霉素1-3份、琼脂20-30份、活性炭5-15份、水40-50份。
2.根据权利要求1所述的风兰专用培养基，其特征在于，由按照重量份计的以下物质制备得到：B5培养基75份、硫酸铁18份、EDTA二钠9份、蔗糖7份、葡萄糖7份、水解乳蛋白9份、玉米胚乳10份、西红柿汁9份、椰子汁8份、吲哚乙酸4份、吲哚丁酸5份、萘氧乙酸5份、ABT生根粉3份、玉米素2份、激动素2份、异戊烯氨基嘌呤2份、赤霉素2份、琼脂25份、活性炭10份、水45份。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052703A (zh) * | 2015-08-13 | 2015-11-18 | 江苏碧云天农林科技有限公司 | 一种风兰的栽培方法 |
| CN106106167A (zh) * | 2016-07-15 | 2016-11-16 | 四川天艺优境环境科技有限公司 | 一种风兰的组织培养快速繁殖方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05336955A (ja) * | 1992-06-11 | 1993-12-21 | Nakano Vinegar Co Ltd | 植物組織培養培地及び該培地を使用する生育方法 |
| CN1404724A (zh) * | 2002-11-07 | 2003-03-26 | 中国科学院武汉植物研究所 | 春兰的快速繁殖方法 |
| CN102976848A (zh) * | 2012-11-30 | 2013-03-20 | 富阳市文曲生态农业开发有限公司 | 一种铁皮石斛培养液 |
-
2014
- 2014-10-24 CN CN201410574241.7A patent/CN104396741B/zh not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05336955A (ja) * | 1992-06-11 | 1993-12-21 | Nakano Vinegar Co Ltd | 植物組織培養培地及び該培地を使用する生育方法 |
| CN1404724A (zh) * | 2002-11-07 | 2003-03-26 | 中国科学院武汉植物研究所 | 春兰的快速繁殖方法 |
| CN102976848A (zh) * | 2012-11-30 | 2013-03-20 | 富阳市文曲生态农业开发有限公司 | 一种铁皮石斛培养液 |
Non-Patent Citations (3)
| Title |
|---|
| 于永畅等: "国兰组织培养研究进展", 《山东林业科技》, no. 4, 31 December 2012 (2012-12-31), pages 100 - 103 * |
| 庞惠仙等: "春兰、豆瓣兰、莲瓣兰和春剑4种国兰种子萌发特性研究初报", 《西部林业科学》, vol. 39, no. 3, 30 September 2010 (2010-09-30), pages 56 - 60 * |
| 殷静等: "国兰无菌快繁体系的研究进展", 《安徽农业科学》, vol. 38, no. 32, 31 December 2010 (2010-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052703A (zh) * | 2015-08-13 | 2015-11-18 | 江苏碧云天农林科技有限公司 | 一种风兰的栽培方法 |
| CN106106167A (zh) * | 2016-07-15 | 2016-11-16 | 四川天艺优境环境科技有限公司 | 一种风兰的组织培养快速繁殖方法 |
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