CN104396725A - Medium indica rice hybrid seed C Liangyou 300 production method - Google Patents

Abstract

The invention belongs to the field of hybrid rice seed production. According to the present invention, the sterile line C815S and the restoring line ZR300 are adopted as the male parent to produce the hybrid rice seed C Liangyou 300; the production method comprises: selecting inbred lines, carrying out security isolation, sowing at the right moment, adjusting the sowing time, adopting the 920 technology and the artificial assisted pollination, rouging, and harvesting at the right moment, and the hybrid seed of the present invention has characteristics of high yield, stable yield, strong disease resistance, and high biological yield.

Description

The production method of a kind of midle-season indica rice crossbreed C two excellent 300

Technical field:

The present invention relates to paddy rice cross breeding seed production field, relate to the production method of a kind of hybrid rice specifically.

Background technology:

China is Rice Cropping big country, in recent years along with country is to the subsidy policy of Farming, and the growth of paddy purchasing price, Monitoring of Paddy Rice Plant Area has had stable increase.Within 2011, according to Ministry of Agriculture's investigation, national Monitoring of Paddy Rice Plant Area increased by 1.9% than upper one year, and wherein early rice cultivated area reaches 8,869 ten thousand mu, increases by 1,750,000 mu than last year.The per mu yield of paddy rice is also along with the popularization of new rice variety and breeding corresponding cultivation techniques is constantly brought forth new ideas height.

But China is populous nation, the four corners of the world, Rice Cropping region is vast in territory, and plantation climatic zone is various.And in recent years, the natural calamity of China takes place frequently, and damage by disease and insect is occurred frequently, diversity state.Breeding high-yield, wide suitable, many anti-new rice variety are the important leverages ensureing Food Security in China.

And along with the establishment of company of exotic industry, the introducing of its external advanced breeding technique and rice pest insects, in order to ensure the safety in rice in China market, selects outstanding hybrid rice seed, it is also the extremely urgent task of rice in China breeding man.

Summary of the invention:

The object of the invention is to for ensureing the New Hybrid Rice Combination that our grain security and rice in China market safety are cultivated, and a kind of production method of producing the hybrid rice seeds of high yield is provided.

Hybrid rice seeds production method provided by the present invention: comprise selection inbred line, Secure isolation, begin sowing in good time, regulate date of seeding, " 920 " technology of use and artificial supplementary pollination, roguing, gathers in the crops in good time, utilizes male sterile line " C815S " for maternal, restorer " ZR300 " is male parent, produces hybrid rice seed " C two excellent 300 ".

Hybrid rice kind " C two excellent 300 " is for breeding goal and the rice varieties of seed selection with Xian main producing region in In Middle And Lower Reaches of Changjiang River.

For selecting rice varieties of the present invention, 30 hybrid rice seeds that company scientific research personnel selects 30 hybrid rice seeds at present before rank is promoted in this region and golden agriculture China to select totally 60 hybrid rice seeds carry out variety comparative test.Gone out by test data analyzer that the yielding ability of hybrid rice seed in this area of rice sterile line " C815S " assembly is good, disease resistance strong, comprehensive resistance is good.

Restorer ZR300 is by the seed selection voluntarily of golden Nong Hua company, and this recovery is the new restorer selected for 2013, ZR300 through golden agriculture China Internal Experiment system, test, assembly, the discovery period restorative good, resistance, rice are of fine quality.

Therefore golden Nong Hua company is maternal with male sterile line " C815S ", with restorer " ZR300 " for male parent; Produce hybrid rice kind " C two excellent 300 ".

Wherein said biomaterial " C815S " draws from Agricultural University Of Hunan, and this kind has obtained New species right in plant protection, kind power CNA:20040515.2.

Wherein said biomaterial restorer " ZR300 " this kind has applied for that New species right in plant is protected, application number: 20140200.8.

The present invention utilizes " C815S " to be maternal, and the feature of the hybrid rice seed " C two excellent 300 " produced for male parent with " ZR300 " is:

This kind plant type is moderate, and leaf look dark green, and sword-like leave is straight and upright, and growing way is in great numbers, and tillering ability is strong, and effective fringe is many, and bran point purple, grain number per spike is many, and ripening rate is high, and thousand kernel weight is little, and ripe phase annesl is good.Plant height 95 centimetres, the effective fringe 24. ten thousand of mu, the percentage of earbearing tiller 55.%, every total grain panicle number 135., Defined daily doses 117, ripening rate 83.%, thousand kernel weight 26 grams.Brown rice yield 83.%, polished rice rate 75.%, head rice rate 65.5%, grain length 6.5mm, grain type aspect ratio 3.2, chalky grain rate 35%, chalkiness degree 4.5%, amylose 15.0%, gel consistence 59mm.

Within 2012, participate in the comparison experiment of company's Content Organizing, average yield per mu 588 kilograms, increase production 9.6% than contrast Yue You 9113; Within 2013, participate in intra-company's comparison experiment, 584.9, than contrast volume increase 9.1%.See attached list 1, subordinate list 2.

Good effect of the present invention is:

1, output is high, and output exceedes main breed more than 9.4% on Vehicles Collected from Market;

2, resistance, middle blast resisting, high against-plant hopper and leaf blight;

3, wide adaptability, is adapted at the middle and lower reach of Yangtze River and South China and plants manyly.

Embodiment

This embodiment is for illustration of the present invention below, but is not used for limiting the scope of the invention.

Embodiment 1: the concrete Breeding Process of crossbreed of the present invention " C two excellent 300 "

From 2010, golden Nong Hua company was collected in the rice varieties of first 50 of the In Middle And Lower Reaches of Changjiang River market ranking, and selected 50 hybrid rice varieties of golden Nong Hua company assembly voluntarily, carried out Engineering Breeding seed selection engineering.

Namely basis is by the plantation place of Cultivars and the weather conditions of place planting site, and damage by disease and insect segmentation breeding objective is swung to and elected.In Hainan, Guangdong, Changsha, Chongqing, Chengdu, Wuhan, six peaces, 10 experiment centres such as Hefei carry out field plot test experiment to the kind of collecting, and analyze fully experimental data.

By analyzing the hybrid rice seed of planting experiment and the disease or pest resistance of parent thereof and applicable weather conditions; And the coordinate force of parent is identified.Select " C815S " and carry out assembly with " ZR300 ", produce crossbreed rice paddy seed " C two excellent 300 ".

The rice paddy seed " C two excellent 300 " that the present invention produces participated in intra-company's comparison experiment at 2012,2013, shows high yield, yielding ability is outstanding, global resistance is strong, than check variety Yue You 9113 on average volume increase 9.4%.

2, output performance:

1) within 2012, participate in the comparison experiment of company's Content Organizing, average yield per mu 588 kilograms, increase production 9.6% than contrast Yue You 9113;

2) within 2013, intra-company's comparison experiment is participated in, average yield per mu 584.9, than contrast volume increase 9.1%.See attached list 1, subordinate list 2

Table 1 each test site yield data unit of company: KG in 2012

Sequence number	Test location	Per mu yield	Contrast Yue You 9113	Than CK ±
					1	Sanya, Hainan experiment station	615.2	526.5	16.8
2	Experiment station of Guangzhou Guangdong	569.8	520.4	9.5
					3	Changsha experiment station	578.5	508.5	13.8
4	Chengdu, Sichuan experiment station	601.5	570.5	5.4
					5	Wuhan, Hubei experiment station	600.5	540.5	11.1
6	Yancheng, Jiangsu Province experiment station	598.5	538.6	11.1
					7	Nanchang experiment station	578.5	525.5	10.1
8	Yichuan experiment station	568.9	565.2	0.7
					9	Shangrao, Jiangxi experiment station	598.5	550.6	8.7
10	Jing Zhou, Hubei experiment station	569.8	525.2	8.5
					On average		588	537.2	9.6

Table 2 each test site yield data unit of company: KG in 2013

Sequence number	Test location	Per mu yield	Contrast Yue You 9113	Than CK ±
					1	Sanya, Hainan experiment station	605.5	515.2	17.5
2	Experiment station of Guangzhou Guangdong	578.5	520.4	11.2
					3	Changsha experiment station	550.5	508.5	8.3
4	Chengdu, Sichuan experiment station	578.2	570.5	1.3
					5	Wuhan, Hubei experiment station	596.5	540.5	10.4
6	Yancheng, Jiangsu Province experiment station	605.2	538.6	12.4
					7	Nanchang experiment station	588.5	530.6	10.9
8	Yichuan experiment station	569.8	555.5	2.6
					9	Shangrao, Jiangxi experiment station	600.5	545.6	10.1
10	Jing Zhou, Hubei experiment station	575.6	539.5	6.7
					On average		584.9	536.5	9.1

Embodiment 2: the production technology of crossbreed of the present invention

1) Secure isolation: space length >500 rice, or other rice varieties of more than 15 days heading flowering periods of being separated by carries out production of hybrid seeds isolation.

2) begin sowing in good time: first phase male parent was than maternal Zao slotting 25 ~ 30 days, or leaf order difference 6-7 leaf, or effective accumulated temperature Parent differs from 200 DEG C, and the second phase, the third phase are separated by with the first phase 7 days respectively, or two phase male parents are separated by 10 days.

3) rational close planting, be suitable for row ratio.Female parent strain line-spacing is 16.7 × 10 centimetres, and male parent spacing in the rows is 16.7 centimetres, and 33.3 centimetres, aisle when duplicate rows is planted, parental is 1:10 ~ 12.

4) " 920 " technology and artificial supplementary pollination is used.Spray 920 during the heading of 5%-10% female parent, every mu sprays 20 grams for the first time, sprays 3-5 days continuously afterwards, each spray 5 grams, total consumption 30-45 gram.Pollen is caught up with to carry out artificial supplementary pollination whole flowering stage every day at florescence from fringe phase beginning to neat fringe.

5) kernel smut technology is prevented and treated: prevent and treat kernel smut continuously 2-3 times with black spirit of going out (producer's medicine) at cut phase, fringe phase beginning and full heading time.

6) roguing technology: pull out form and maternal " C815S " discrepant plant whole breeding time, contain and spend latter about 25 days to gather in the crops hybrid seed.

Embodiment 3: the culture technique of crossbreed of the present invention

1) begin sowing in good time, cultivate strong seedling.According to working as ground temperature light resources reasonable arrangement date of seeding.Mu sowing quantity 1-1.5KG, rice shoot one leaf one heart stage, sprays paclobutrazol and strengthens seedling and urge tiller, thinly evenly to broadcast, and is advisable within 35 days length of time rice seedlings grow.First-class fertility field seeding row spacing 5 × 8 cun, below average fertility field seeding row spacing 4 × 9 cun, guarantees several seedling 8-10 ten thousand.

2) heavy base fertilizer application is answered in fertilising, and early chase after tillering fertilizer, mend potassium application after dry field rehydration, the general mu in land for growing field crops executes purity nitrogen 13-15 KG.Mid-term is seedling dry field enough, and later stage alternation of wetting and drying, gathers in the crops the first seven sky and cut off the water supply, gather in good time.

3) damage by disease and insect such as attention control rice blast, false smut.

Claims (7)

1. a production method of midle-season indica rice crossbreed C two excellent 300, is characterized in that selecting rice sterile line " C815S " to do maternal, does male parent, produce hybrid rice seeds " C two excellent 300 " with rice restorer " ZR300 ".

2. method according to claim 1, is characterized in that Secure isolation distance >=500 meters, or is separated by more than 15 days with the heading flowering period of described Parent and carries out production of hybrid seeds isolation.

3. method according to claim 1, interval sowing, is characterized in that first phase male parent is than maternal Zao slotting 25-30 days, or leaf age difference 6-7 leaf, or effective accumulated temperature Parent differs from 200 DEG C, the second phase, the third phase are separated by with the first phase 7 days respectively, or two phase male parents are separated by 10 days.

4. method according to claim 1, it is characterized in that female parent strain line-spacing is 16.7 × 10 centimetres, male parent spacing in the rows is 16.7 centimetres, and 33.3 centimetres, aisle when duplicate rows is planted, parental is 1:10 ~ 12.

5. method according to claim 1, spray 920 when it is characterized in that the heading of 5%-10% female parent, every mu sprays 20 grams for the first time, sprays 3-5 days continuously afterwards, each spray 5 grams, total consumption 30-45 gram.

6. method according to claim 1, is characterized in that preventing and treating kernel smut continuously 2-3 times at cut phase, fringe phase beginning and full heading time.

7. the method according to claim l, is characterized in that pulling out External Characters and maternal " C815S " discrepant plant whole breeding time, contains and spend latter 25 days results hybrid seeds.