CN104383599A - Collagen-based cornea scaffold and preparation method thereof - Google Patents
Collagen-based cornea scaffold and preparation method thereof Download PDFInfo
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- CN104383599A CN104383599A CN201410662542.5A CN201410662542A CN104383599A CN 104383599 A CN104383599 A CN 104383599A CN 201410662542 A CN201410662542 A CN 201410662542A CN 104383599 A CN104383599 A CN 104383599A
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Abstract
The invention relates to a collagen-based cornea scaffold and a preparation method thereof, belonging to the field of medical biomaterials. The matrix is collagen, composite procyanidine or procyanidine, anthocyanin or procyanidine is added by a dialysis method, and the transparent 100-800 mu m thick multilayer film scaffold prepared by a specific process is used as a substitute of a human cornea transplantation donor material in a cornea transplantation or repair operation. The cornea scaffold material is used as an attachment scaffold of epithelial cells or endothelial cells in cornea transplantation or repair operation, provides nutrition support for the growth of the cells, has good transparency and high mechanical strength by fusing anthocyanin or procyanidine and collagen and forming a parallel multilayer cornea scaffold structure by crosslinking and electrophoresis technology, ensures that the cornea scaffold material can completely replace or partially perform the physiological function of human eyes, and ensures that patients can be well-known or have treatment effect.
Description
Technical field
The present invention relates to a kind of collagen scaffold material for cornea and preparation method thereof, belong to biomaterial for medical purpose field.
Background technology
Cornea is that eyes front end one deck is completely transparent, in horizontal oval thin film.Occupy an important position in eye refraction, also provide special protective effect to meticulous eyeball content together with scleral tissue simultaneously.Cornea can be divided into 5 layers structure, and middle one deck is hypothallus, accounts for 90% of whole corneal thickness, forms primarily of collagen fiber, adhesion substance and keratinocyte.The arrangement of collagen fibers of hypothallus is regular, evenly, slabbing, is closely stacked layer by layer, and each fibrous layer becomes again decussation to arrange.This architectural feature of fibrous layer arrangement is conducive to light and passes through and flexion.Many patients cause blind because of cornea disease, and the feasible solution that such patient recovers lost eyesight carries out corneal graft, but cornea source all comes from non-human donor's donation at present.Donor source is limited is restrict to implement corneal graft and successful bottleneck with immunological rejection, causes many cornea Diseases to be in for a long time and waits in the middle of donor donation and blind misery.Therefore develop a kind of convenient sources, alternative donor contributes cornea, and the artificial cornea that immunogenicity is low just becomes especially important and urgent.
Procyanidin and anthocyanidin are all potent free radical scavengers.Procyanidin also has the effect of protease inhibitor, and procyanidin is connected to after on collagen protein, and collagenase and elastoser etc. can be stoped collagen protein decomposition.Procyanidin can not only help collagen fabric to form cross-linked structure simultaneously, also can help to recover the excessively crosslinked infringement because of caused by injured and free radical.Procyanidin can also strengthen the microcirculation of eye ground blood capillary and extremity tip blood capillary, reduces eyes capillary hemorrhage, protects and improve vision.Anthocyanidin plays inhibitory action to elastoser and Collagenase, and energy and protein bound prevent peroxidating, can also alleviate eye strain simultaneously, accelerates vitamin A and synthesizes rhodopsin on the retina, pre-myopia prevention, promote vision.
Collagen scaffold material for cornea provided by the invention, utilize bionical principle, simulate the composition and structure feature of natural mankind's cornea as far as possible, with Atelocollagen albumen for substrate, compound procyanidin or anthocyanidin is added by the method for dialysis, make the two together with the abundant Hybrid connections of collagen protein, play the effect to collagen protein protection, stop protease to the decomposition of collagen protein, extend the degradation time of collagen cornea, can also play simultaneously and strengthen ocular angiogenesis microcirculation, alleviate eye strain, protect and improve vision, certain facilitation is played in recovery after corneal transplanting.High, the transparent membrane structure of multilamellar, collagen fibrils orient queueing discipline, mechanical strength is formed additionally by the method such as gelation, hardening, electrophoresis, fully close to " arrangement of collagen fibers of hypothallus is regular, even; slabbing; be closely stacked layer by layer; each fibrous layer becomes again decussation to arrange " architectural feature of natural mankind's cornea, so can closer to the natural function of eye cornea.Take collagen protein as the collagen canthus thickness 100 ~ 800 μm of substrate, there is multiple structure, after crosslinked by double crosslinker procyanidin or anthocyanidin, EDC, mechanical strength is high, degradation cycle is long, light transmission is good, and two sides is all beneficial to cell adhesion growth, after operation transplantation, can substitute or partly exercise the physiological function of eye cornea completely, patient is recovered lost eyesight or plays therapeutic effect.
Summary of the invention
The invention belongs to biomaterial for medical purpose field, relate to a kind of collagen scaffold material for cornea and preparation method thereof.The substrate of this collagen scaffold material for cornea is collagen protein.
The object of the present invention is to provide a kind of preparation method of collagen scaffold material for cornea, this timbering material is made up of collagen protein, dialysis process is used to add anthocyanidin or procyanidin, by transparent, 100 ~ 800 μm of thick multilayer film supports that special process is prepared into, as the succedaneum of the people's corneal transplantation donor material in corneal transplantation or repairing operation.This cornea scaffold is as the steel framework of epithelial cell or endotheliocyte in corneal transplantation or kposthesis, and for the growth of cell provides nutritional support, the DMEM culture medium of simultaneously adding in CF is shaped may further be Growth of Cells and provides support.By anthocyanidin or procyanidin and collagen protein are merged, and by parallel multilayer angle membrane support structure that is crosslinked and electrophoretic techniques formation, transparency is good, mechanical strength is high, ensure that it can substitute completely or partly exercise the physiological function of eye cornea, patient is recovered lost eyesight or plays therapeutic effect.
The present invention relates to a kind of multilamellar, transparent collagen scaffold material for cornea.
The invention particularly relates to a kind of cornea scaffold of implant form.
A kind of collagen scaffold material for cornea, is characterized in that basis is collagen protein, adds compound procyanidin, Anthocyanins, the transparent bionical cornea support of the multiple structure made by special process.
A kind of collagen-based cornea support and preparation method thereof, it is characterized in that collagen protein be enzyme action go to hold in the I-type collagen of peptide, type III collagen protein one or both, derive from beef tendon, abortive calfskin, Corii Sus domestica, fish skin, Jellyfish, Stichopus japonicus one or more.
A kind of collagen-based cornea support and preparation method thereof, it is characterized in that collagen-based cornea is formed by stacking so that the orthogonal collagen fiber layer of multilamellar is crosslinked, film thickness reaches 100 ~ 800 μm, transparent, and mechanical strength is high.
A kind of collagen scaffold material for cornea, it is characterized in that the procyanidin ingredient origin comprised is in Semen Vitis viniferae, blue berry, Flos Rosae Rugosae, Anthocyanins derives from Pericarpium Vitis viniferae, blue berry.
A preparation method for collagen scaffold material for cornea, specifically comprises the following steps.
(1) get collagen protein, be dissolved in the acetum of 0.5mol/L, be mixed with the collagen solution that final concentration is 0.5 ~ 20g/L.Load bag filter, be placed in extracellular fluid dialysis and carry out dialysis 2 days.
(2) collagen solution that above-mentioned dialysis is complete with add 10mmol/L HEPES without phenol red DMEM culture fluid with (1 ~ 10): the ratio of (10 ~ 1) is mixed homogeneously, and obtains solution A.
(3) the above-mentioned solution A plastic film mulch (20*20mm) of 1ml is got.
(4) incubator is placed in, 37 DEG C, 5% carbon dioxide hatches 2h, to collagen gel.Under aseptic condition, carry out drying subsequently, change hard glassy thin film into collagen gel.
(5) 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides (EDC) solution adding 2ml 0.1 ~ 10g/L on above-mentioned thin film is cross-linked, and then outwells EDC solution, 95% ethanol rinse 2 times, and purified water is washed.
(6) outwell purified water, dry, then add 0.7ml solution A, crosslinked 1h, carries out electrophoresis 1 ~ 60min along direction 80mV, a 10mA.
(7) step 4 is repeated.
(8) repeat step 5,6,7 to collagem membrane thickness reaches 100 ~ 800 μm.Often repeat once, electrophoresis direction is perpendicular to a upper circulation electrophoresis direction.
(9) film is taken off, Co60 irradiation or ethylene oxide sterilizing.
Extracellular fluid dialysis according to above-mentioned steps (1) is the acetum of 0.5mol/L or with the addition of the acetum of one or both the 0.5mol/L in procyanidin, anthocyanidin two kinds of compositions.
Procyanidin in extracellular fluid dialysis according to above-mentioned steps (1), the concentration of anthocyanidin are 0.01 ~ 5g/L.
A kind of collagen-based cornea support and preparation method thereof, it is characterized in that by dialysis interpolation, the connection of procyanidin or anthocyanidin and collagen protein being merged in preparation process more abundant, make every layer of collagen fibrils orient queueing discipline by the method for electrophoresis, adjacent two layers collagen fiber electrophoresis direction vertically ensure that collagen fiber layer becomes decussation to superpose simultaneously.
A kind of collagen-based cornea support and preparation method thereof, it is characterized in that its light transmittance of collagen cornea is 89%-97%, mechanical strength is 10-40MPa.
A kind of collagen-based cornea support and preparation method thereof; it is characterized in that collagen cornea support can be applied to the treatment of the human body comprising cornea disease various film class disease as a kind of medical apparatus and instruments; also can to grow as providing cell adhesion and the tissue engineering bracket material playing supportive protection effect uses, or in order to cultured cell.
Detailed description of the invention
Embodiment 1
(1) get collagen protein, be dissolved in the acetum of 0.5mol/L, be mixed with the collagen solution that final concentration is 0.5g/L.Load bag filter, the acetum being placed in 0.5mol/L carries out dialysis 2 days.
(2) collagen solution that above-mentioned dialysis is complete with add 10mmol/L HEPES without phenol red DMEM culture fluid with 1: 10 ratio mix homogeneously, obtain solution A.
(3) the above-mentioned solution A plastic film mulch (20*20mm) of 1ml is got.
(4) incubator is placed in, 37 DEG C, 5% carbon dioxide hatches 2h, to collagen gel.Under aseptic condition, carry out drying subsequently, change hard glassy thin film into collagen gel.
(5) 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides (EDC) solution adding 2ml 0.1g/L on above-mentioned thin film is cross-linked, and then outwells EDC solution, 95% ethanol rinse 2 times, and purified water is washed.
(6) outwell purified water, dry, then add 0.7ml solution A, crosslinked 2h, carries out electrophoresis 5min along direction 80mV, a 10mA.
(7) step 4 is repeated.
(8) repeat step 5,6,7, electrophoresis direction is perpendicular to last electrophoresis direction.
(9) film is taken off, Co60 irradiation.
Embodiment 2
(1) get collagen protein, be dissolved in the acetum of 0.5mol/L, be mixed with the collagen solution that final concentration is 5g/L.Load bag filter, be placed in extracellular fluid dialysis and carry out dialysis 2 days.
(2) collagen solution that above-mentioned dialysis is complete with add 10mmol/L HEPES without phenol red DMEM culture fluid with 5: 5 ratio mix homogeneously, obtain solution A.
(3) the above-mentioned solution A plastic film mulch (20*20mm) of 1ml is got.
(4) incubator is placed in, 37 DEG C, 5% carbon dioxide hatches 2h, to collagen gel.Under aseptic condition, carry out drying subsequently, change hard glassy thin film into collagen gel.
(5) 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides (EDC) solution adding 2ml 5g/L on above-mentioned thin film is cross-linked, and then outwells EDC solution, 95% ethanol rinse 2 times, and purified water is washed.
(6) outwell purified water, dry, then add 0.7ml solution A, crosslinked 2h, carries out electrophoresis 30min along direction 80mV, a 10mA.
(7) step 4 is repeated.
(8) step 5,6,7 is repeated, 20 times.Often repeat once, electrophoresis direction is perpendicular to a upper circulation electrophoresis direction.
(9) film is taken off, Co60 irradiation.
Embodiment 3
(1) get collagen protein, be dissolved in the acetum of 0.5mol/L, be mixed with the collagen solution that final concentration is 10g/L.Load bag filter, be placed in extracellular fluid dialysis and carry out dialysis 2 days.
(2) collagen solution that above-mentioned dialysis is complete with add 10mmol/L HEPES without phenol red DMEM culture fluid with 10: 1 ratio mix homogeneously, obtain solution A.
(3) the above-mentioned solution A plastic film mulch (20*20mm) of 1ml is got.
(4) incubator is placed in, 37 DEG C, 5% carbon dioxide hatches 2h, to collagen gel.Under aseptic condition, carry out drying subsequently, change hard glassy thin film into collagen gel.
(5) 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides (EDC) solution adding 2ml 10g/L on above-mentioned thin film is cross-linked, and then outwells EDC solution, 95% ethanol rinse 2 times, and purified water is washed.
(6) outwell purified water, dry, then add 0.7ml solution A, crosslinked 2h, carries out electrophoresis 60min along direction 80mV, a 10mA.
(7) step 4 is repeated.
(8) step 5,6,7 is repeated, 50 times.Often repeat once, electrophoresis direction is perpendicular to a upper circulation electrophoresis direction.
(9) film is taken off, Co60 irradiation.
Embodiment 4 cell toxicity test
(1) cell culture
Mouse embryo fibroblasts system (3T3, ATCC) applies DMEM culture medium (containing 1% dual anti-and 10% calf serum), 37 DEG C, cultivate in 5% carbon dioxide cell incubator.
(2) MTT test
The cell suspending liquid of draws equal amounts, joins in 24 porocyte culture plates that collagen-based cornea directly contacts.Negative control adds PBS, and positive control adds DMSO (final concentration is 10%), and blank group for only having cell culture medium.Cultivate and carry out MTT test afterwards in 24 hours.The light absorption value (OD value) in each hole is measured with enzyme-linked immunosorbent assay instrument OD 570nm place.Be calculated as follows cell inhibitory rate: cell inhibitory rate=(1-processed group absorbance/blank group absorbance) × 100%.
MTT tests:
Except positive controls, all the other two groups compared with blank group, all non-on cell proliferation produces and suppresses reaction.T checks, * * * P < 0.001.
Embodiment 5 zoopery
Laboratory animal: new zealand white rabbit.
Rabbit age: the 4-5 month.
(1) muscular grafting
Collagen-based cornea normal saline is soaked 30min, is cut into the lamellar of 10mm*3mm.Sheet-form collagenous base cornea is implanted White Rabbit back right muscles, and blank is done in left side.Operation stitching, continuous three days injection 800,000 units of Penicillin.
(2) experimental result
Started respectively at postoperative 2,4,6,8,10,12,14,16,18,20,22,24 weeks and get embedded material peripheral muscle tissue, carry out Physico-chemical tests, be the index that notes abnormalities.
Start after drawing materials, calculate the degradation rate of embedded material, the collagen-based cornea material that result is implanted in degraded in postoperative 14 weeks completely.
Claims (9)
1. collagen-based cornea support and preparation method thereof, is characterized in that this collagen cornea support basis is collagen protein, adds compound procyanidin, Anthocyanins.
2. a kind of collagen-based cornea support according to claim 1 and preparation method thereof, it is characterized in that described collagen protein be enzyme action go to hold in the I-type collagen of peptide, type III collagen protein one or both, derive from beef tendon, abortive calfskin, Corii Sus domestica, fish skin, Jellyfish, Stichopus japonicus one or more.
3. a kind of collagen-based cornea support according to claim 1 and preparation method thereof, it is characterized in that this collagen-based cornea is formed by stacking so that the orthogonal collagen fiber layer of multilamellar is crosslinked, film thickness reaches 100 ~ 800 μm, transparent, and mechanical strength is high.
4. a kind of collagen-based cornea support according to claim 1 and preparation method thereof, is characterized in that preparation method comprises the following steps:
(1) get collagen protein, be dissolved in the acetum of 0.5mol/L, be mixed with the collagen solution that final concentration is 0.5 ~ 20g/L.Load bag filter, be placed in extracellular fluid dialysis and carry out dialysis 2 days;
(2) collagen solution that above-mentioned dialysis is complete with add 10mmol/L HEPES without phenol red DMEM culture fluid with (1 ~ 10): the ratio of (10 ~ 1) is mixed homogeneously, and obtains solution A;
(3) the above-mentioned solution A plastic film mulch (20*20mm) of 1ml is got;
(4) incubator is placed in, 37 DEG C, 5% carbon dioxide hatches 2h, to collagen gel.Under aseptic condition, carry out drying subsequently, change hard glassy thin film into collagen gel;
(5) 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides (EDC) solution adding 2ml 0.1 ~ 10g/L on above-mentioned thin film is cross-linked, and then outwells EDC solution, 95% ethanol rinse 2 times, and purified water is washed;
(6) outwell purified water, dry, then add 0.7ml solution A, crosslinked 1h, carries out electrophoresis 1 ~ 60min along direction 80mV, a 10mA.
(7) step 4 is repeated;
(8) repeat step 5,6,7 to collagem membrane thickness reaches 100 ~ 800 μm.Often repeat once, electrophoresis direction is perpendicular to a upper circulation electrophoresis direction;
(9) film is taken off, Co60 irradiation or ethylene oxide sterilizing.
5. extracellular fluid dialysis according to claim 4 is the acetum of 0.5mol/L or with the addition of the acetum of one or both the 0.5mol/L in procyanidin, anthocyanidin two kinds of compositions.
6. the procyanidin in extracellular fluid dialysis according to claim 4, the concentration of anthocyanidin are 0.01 ~ 5g/L.
7. a kind of collagen-based cornea support according to claim 4 and preparation method thereof, it is characterized in that by dialysis interpolation, the connection of procyanidin or anthocyanidin and collagen protein being merged in preparation process more abundant, make every layer of collagen fibrils orient queueing discipline by the method for electrophoresis, adjacent two layers collagen fiber electrophoresis direction vertically ensure that collagen fiber layer becomes decussation to superpose simultaneously.
8. a kind of collagen-based cornea support according to claim 1 or 4 and preparation method thereof, it is characterized in that its light transmittance of collagen cornea is 89%-97%, mechanical strength is 10-40MPa.
9. a kind of collagen-based cornea support according to claim 1 and preparation method thereof; it is characterized in that collagen cornea support can be applied to the treatment of the human body comprising cornea disease various film class disease as a kind of medical apparatus and instruments; also can to grow as providing cell adhesion and the tissue engineering bracket material playing supportive protection effect uses, or in order to cultured cell.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110613865A (en) * | 2019-11-07 | 2019-12-27 | 四川大学 | Preparation and storage method of biological valve material subjected to combined treatment of carbodiimide and polyphenol |
| CN113773379A (en) * | 2021-09-13 | 2021-12-10 | 熹微(苏州)生物医药科技有限公司 | Method for preparing polyethylene glycol collagen and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008115160A1 (en) * | 2007-03-19 | 2008-09-25 | Vasif Hasirci | Stacked, patterned biomaterials and/or tissue engineering scaffolds |
| CN102908666A (en) * | 2012-10-11 | 2013-02-06 | 天津市赛瑞生物技术有限公司 | Collagen scaffold material for cornea |
| US20130230573A1 (en) * | 2010-11-16 | 2013-09-05 | Oded Shoseyov | Collagen structures and method of fabricating the same |
| CN103384536A (en) * | 2011-02-21 | 2013-11-06 | 株式会社阿托利 | Collagen material and method for producing collagen material |
| WO2014074134A1 (en) * | 2012-11-09 | 2014-05-15 | Tufts University | Multilayered collagen scaffold |
-
2014
- 2014-11-05 CN CN201410662542.5A patent/CN104383599A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008115160A1 (en) * | 2007-03-19 | 2008-09-25 | Vasif Hasirci | Stacked, patterned biomaterials and/or tissue engineering scaffolds |
| US20130230573A1 (en) * | 2010-11-16 | 2013-09-05 | Oded Shoseyov | Collagen structures and method of fabricating the same |
| CN103384536A (en) * | 2011-02-21 | 2013-11-06 | 株式会社阿托利 | Collagen material and method for producing collagen material |
| CN102908666A (en) * | 2012-10-11 | 2013-02-06 | 天津市赛瑞生物技术有限公司 | Collagen scaffold material for cornea |
| WO2014074134A1 (en) * | 2012-11-09 | 2014-05-15 | Tufts University | Multilayered collagen scaffold |
Non-Patent Citations (1)
| Title |
|---|
| 林旭明等: ""原花青素交联的支架材料的生物相容性研究"", 《眼科新进展》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110613865A (en) * | 2019-11-07 | 2019-12-27 | 四川大学 | Preparation and storage method of biological valve material subjected to combined treatment of carbodiimide and polyphenol |
| CN113773379A (en) * | 2021-09-13 | 2021-12-10 | 熹微(苏州)生物医药科技有限公司 | Method for preparing polyethylene glycol collagen and application thereof |
| CN113773379B (en) * | 2021-09-13 | 2023-08-01 | 熹微(苏州)生物医药科技有限公司 | Method for preparing polyethylene glycol collagen-like protein and application thereof |
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