CN104374906B - A kind of serum ammonia detection reagent - Google Patents
A kind of serum ammonia detection reagent Download PDFInfo
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- CN104374906B CN104374906B CN201410697748.1A CN201410697748A CN104374906B CN 104374906 B CN104374906 B CN 104374906B CN 201410697748 A CN201410697748 A CN 201410697748A CN 104374906 B CN104374906 B CN 104374906B
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- ammonia
- ammonia detection
- damping fluid
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 100
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 97
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract description 50
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 210000002966 serum Anatomy 0.000 title claims abstract description 26
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 claims abstract description 38
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 claims abstract description 38
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 17
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 17
- 239000002105 nanoparticle Substances 0.000 claims abstract description 17
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims abstract description 16
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000007983 Tris buffer Substances 0.000 claims abstract description 16
- 238000013016 damping Methods 0.000 claims abstract description 16
- 239000012530 fluid Substances 0.000 claims abstract description 16
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 claims abstract description 13
- 101710088194 Dehydrogenase Proteins 0.000 claims abstract description 13
- 102000016901 Glutamate dehydrogenase Human genes 0.000 claims abstract description 13
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims abstract description 11
- 229950006238 nadide Drugs 0.000 claims abstract description 11
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000008367 deionised water Substances 0.000 claims description 14
- 229910021641 deionized water Inorganic materials 0.000 claims description 14
- 229910001566 austenite Inorganic materials 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 description 15
- 239000007788 liquid Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 9
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229940107700 pyruvic acid Drugs 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 208000014644 Brain disease Diseases 0.000 description 2
- 208000032274 Encephalopathy Diseases 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000001934 delay Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 208000007788 Acute Liver Failure Diseases 0.000 description 1
- 206010000804 Acute hepatic failure Diseases 0.000 description 1
- 206010010075 Coma hepatic Diseases 0.000 description 1
- 206010020575 Hyperammonaemia Diseases 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- 208000001940 Massive Hepatic Necrosis Diseases 0.000 description 1
- 201000007981 Reye syndrome Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 201000001059 hepatic coma Diseases 0.000 description 1
- 208000007386 hepatic encephalopathy Diseases 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 238000011430 maximum method Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Abstract
The invention discloses a kind of serum ammonia detection reagent, comprise reagent 1 and reagent 2 that volume ratio is 6:1.Wherein reagent 1 comprises the tris damping fluid of pH=8.0, serum lactic dehydrogenase, nanoparticle, ethylenediamine tetraacetic acid (EDTA), reduced coenzyme Ⅰ, bovine serum albumin and adenosine diphosphate (ADP); Reagent 2 comprises the tris damping fluid of pH=8.0, α-ketoglutaric acid, glutamate dehydrogenase, bovine serum albumin and adenosine diphosphate (ADP).Reagent of the present invention is double reagent, and have easy and simple to handle, good stability, accuracy is high, is generally applicable to the excellent properties of the automatic clinical chemistry analyzer of various model, is a kind of novel ammonia detection reagent be worthy to be popularized.
Description
Technical field
The present invention relates to technical field of medical examination, be specifically related to a kind of serum ammonia detection reagent.
Background technology
Blood in human body in ammonia (Plasmaammonia) content is atomic, but ammonia is poisonous to human body, the metabolism of the cell that can affect the nerves.The source of blood ammonia increases and outlet is reduced, and blood ammonia all can be caused to increase.Under normal circumstances, ammonia changes urea in liver.During major Liver disease, ammonia can not be removed from circulation, and cause blood ammonia to increase, hyperammonemia has Nervous toxicity, causes hepatogenic encephalopathy (hepatic coma), and determination of blood ammonia of being therefore grown up is extremely important to the diagnosis of hepatogenic encephalopathy and discriminating.In addition, determination of blood ammonia diagnoses Reye ' s syndrome very useful to paediatrics, and this has levied severe hypoglycemia, massive hepatic necrosis, acute hepatic failure, and with fatty degeneration of liver.Before liver zymogram increases, namely see that blood ammonia increases.
Ammonia method for measuring has microdiffusion, ion exchange method, enzyme process and ammonia electrode method etc.The maximum method of current application is enzyme process and the determination of blood ammonia instrument analytical method based on ion selective electrode.Conventional ammonia detection reagent (glutamate dehydrogenase two-point method) is three reagent, mainly there is following defect: be not 1. suitable for common general full automatic Biochemical Analyzer, the automatic clinical chemistry analyzer used in current medical inspection mechanism only has two reagent discs, and therefore three reagent detection kit directly can not carry out unattended operation; 2. use procedure is loaded down with trivial details.Manually a liquid in reagent 1 and b liquid must be mixed according to a certain percentage before using, just can carry out unattended operation, greatly increase the workload of operator; 3. in manual handling thereof, easily cause misoperation, and there is inevitable operate miss.
Summary of the invention
For overcoming problems of the prior art, the invention provides a kind of serum ammonia detection reagent.This reagent is double reagent, and have easy and simple to handle, good stability, accuracy is high, is generally applicable to the excellent properties of the automatic clinical chemistry analyzer of various model.
The technical solution used in the present invention is as follows:
A kind of serum ammonia detection reagent comprises the reagent 1 and reagent 2 that volume ratio is 6:1;
Described, the component of reagent 1 is:
PH is the tris damping fluid 10-50mmol of 8.0
Serum lactic dehydrogenase 2KU
Nanoparticle 10-50mmol
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol
Reduced coenzyme Ⅰ 0.2g
Bovine serum albumin 0.5-1g
Adenosine diphosphate (ADP) 0.1-0.6mmol
Deionized water adds to 1L;
Described, nanoparticle is γ-Fe
2o
3nanoparticle, its particle diameter is 3-50nm;
Described, the component of reagent 2 is:
PH is the tris damping fluid 10-50mmol of 8.0
α-ketoglutaric acid 30mmol
Glutamate dehydrogenase 25KU
Bovine serum albumin 0.5-1g
Adenosine diphosphate (ADP) 0.1-0.6mmol
Deionized water adds to 1L.
Preferably, the component of reagent 1 is:
PH is the tris damping fluid 30mmol of 8.0
Serum lactic dehydrogenase 2KU
Nanoparticle 25mmol
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol
Reduced coenzyme Ⅰ 0.2g
Bovine serum albumin 0.8g
Adenosine diphosphate (ADP) 0.4mmol
Deionized water adds to 1L.
Preferably, the component of reagent 2 is:
PH is the tris damping fluid 25mmol of 8.0
α-ketoglutaric acid 30mmol
Glutamate dehydrogenase 25KU
Bovine serum albumin 0.7g
Adenosine diphosphate (ADP) 0.4mmol
Deionized water adds to 1L.
Beneficial effect of the present invention:
1) reagent of the present invention adds serum lactic dehydrogenase in reagent 1, and serum lactic dehydrogenase can remove in serum the pyruvic acid disturbing ammonia to measure, γ-Fe
2o
3nanoparticle can strengthen the activity of serum lactic dehydrogenase, makes to react carry out more thorough, effectively removes the interference of pyruvic acid.
2) reagent of the present invention adds the activator of adenosine diphosphate (ADP) as enzyme reaction in reagent 1, the combination of reduced coenzyme Ⅰ to zymophore can be weakened, prevent the excessive suppression caused of reduced coenzyme Ⅰ, the stability of glutamate dehydrogenase in ealkaline buffer is increased, and Reaction time shorten.
3) ammonia detection reagent of the present invention, changes three traditional reagent into double reagent, simplifies the operation course, alleviate the workload of operator, increase work efficiency, be generally applicable to the automatic clinical chemistry analyzer of various model.
4) ammonia detection reagent of the present invention, good stability, accuracy is high, is a kind of novel ammonia detection reagent be worthy to be popularized.
Accompanying drawing explanation
The reagent that Fig. 1 embodiment of the present invention 1 to 3 is obtained and control group reagent stability test-results figure.
Embodiment
For a better understanding of the present invention, further illustrate below in conjunction with specific embodiment.
embodiment 1
A kind of serum ammonia detection reagent comprises the reagent 1 and reagent 2 that volume ratio is 6:1;
The component of reagent 1 is:
PH is the tris damping fluid 10mmol of 8.0
Serum lactic dehydrogenase 2KU
γ-Fe
2o
3nanoparticle 50mmol
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol
Reduced coenzyme Ⅰ 0.2g
Bovine serum albumin 0.5g
Adenosine diphosphate (ADP) 0.6mmol
Deionized water adds to 1L;
The component of reagent 2 is:
PH is the tris damping fluid 50mmol of 8.0
α-ketoglutaric acid 30mmol
Glutamate dehydrogenase 25KU
Bovine serum albumin 0.5g
Adenosine diphosphate (ADP) 0.6mmol
Deionized water adds to 1L.
Wherein, γ-Fe
2o
3the particle diameter of nanoparticle is 3nm.
embodiment 2
A kind of serum ammonia detection reagent comprises the reagent 1 and reagent 2 that volume ratio is 6:1;
The component of reagent 1 is:
PH is the tris damping fluid 30mmol of 8.0
Serum lactic dehydrogenase 2KU
γ-Fe
2o
3nanoparticle 25mmol
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol
Reduced coenzyme Ⅰ 0.2g
Bovine serum albumin 0.8g
Adenosine diphosphate (ADP) 0.4mmol
Deionized water adds to 1L;
The component of reagent 2 is:
PH is the tris damping fluid 25mmol of 8.0
α-ketoglutaric acid 30mmol
Glutamate dehydrogenase 25KU
Bovine serum albumin 0.7g
Adenosine diphosphate (ADP) 0.4mmol
Deionized water adds to 1L.
Wherein, γ-Fe
2o
3the particle diameter of nanoparticle is 28nm.
embodiment 3
A kind of serum ammonia detection reagent comprises the reagent 1 and reagent 2 that volume ratio is 6:1;
The component of reagent 1 is:
PH is the tris damping fluid 50mmol of 8.0
Serum lactic dehydrogenase 2KU
γ-Fe
2o
3nanoparticle 10mmol
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol
Reduced coenzyme Ⅰ 0.2g
Bovine serum albumin 1g
Adenosine diphosphate (ADP) 0.1mmol
Deionized water adds to 1L;
The component of reagent 2 is:
PH is the tris damping fluid 10mmol of 8.0
α-ketoglutaric acid 30mmol
Glutamate dehydrogenase 25KU
Bovine serum albumin 1g
Adenosine diphosphate (ADP) 0.1mmol
Deionized water adds to 1L.
Wherein, γ-Fe
2o
3the particle diameter of nanoparticle is 50nm.
The using method of a kind of serum ammonia detection reagent of the present invention is: select the automatic clinical chemistry analyzer with double reagent function, as Toshiba 40 fully-automatic analyzer, reagent preparation 1 and reagent 2, first reagent 1 is added to automatic clinical chemistry analyzer 37 DEG C of preincubates 3 minutes, again reagent 2 is added in reagent 1,5 minutes are hatched in automatic clinical chemistry analyzer 37 DEG C of delays after mixing, the volume ratio of reagent 1 and reagent 2 is 4:1, the parameter of adjustment Biochemical Analyzer, place distilled water, Landau calibration object and sample at the correspondence position of sample disc, concrete operations are as shown in table 1.
The using method of table 1 ammonia detection reagent
Calculate: ammonia density=(A measures ÷ A standard) × C standard
Note: ammonia calibration object (50 μm of ol/l): take (NH
4)
2sO
4660.7mg, is dissolved in 100ml deammoniation water, makes the stock solution that concentration is 100mm/L, then spend ammoniacal liquor dilution stock solution become 50 μm of ol/l.
The ammonia detection reagent obtained to the embodiment of the present invention 1 to 3 carries out accuracy experiment and stability simultaneous test respectively.
accuracy is tested
Utilize ammonia standardized solution, with conventional ammonia detection reagent (glutamate dehydrogenase two-point method) (below referred to as finished product reagent) in contrast, accuracy validation is carried out to the ammonia detection reagent of embodiment 1 to 3 and finished product test kit.Need before experiment first a liquid in finished product test kit self-contained reagent 1 and the b liquid in reagent 1 to be mixed as reagent 1 in the ratio of 5:1, concrete operation step is: ammonia calibration object (theoretical value is 40 μm of ol/L) is diluted to certain concentration gradient (0 μm of ol/L, 10 μm of ol/L, 20 μm of ol/L, 26 μm of ol/L, 30 μm of ol/L, 40 μm of ol/L) by (1), respectively get 20 μ L, parallelly be divided into 4 groups, add reagent 1 that 240 μ L have mixed respectively and reagent 1,37 DEG C prepared by embodiment 1 to 3 hatches 3min; (2) 5min is hatched in reagent 2, the 37 DEG C of delays adding 40 μ L finished product test kit self-contained reagents 2 and embodiment 1 to 3 preparation more respectively; (3) blank tube zeroing, after 340nm measures absorbance A 1,2min, 340nm measures absorbance A 2, calculates △ A=A2-A1; (4) method of calculation:
Sample △ A
Sample ammonia density=------× normal concentration
Standard △ A
With finished product test kit in contrast, detected result is as shown in table 1.
Table 1 detected result
Above-mentioned relation conefficient, is compared with ammonia reference liquid.
As shown in table 1, the relation conefficient that finished product test kit detection different concns ammonia reference liquid obtains is 0.998, and embodiment 1, embodiment 2 and the test kit detected result relation conefficient prepared by embodiment 3 are respectively 0.997,0.999 and 0.999.The ammonia detection kit prepared of the present invention is suitable in accuracy with finished product test kit as can be seen here, but ammonia detection kit of the present invention uses easier, without the need to a liquid in reagent 1 is mixed with the b liquid in reagent 1 before experiment, greatly save the workload of testing staff, improve working efficiency.
stability test
The conventional constituents that comparative example 1 and 2 is respectively commercially available is the ammonia detection reagent (glutamate dehydrogenase two-point method) of three reagent, needs first a liquid in reagent 1 and the b liquid in reagent 1 to be mixed as reagent 1 in the ratio of 5:1 before experiment.Respectively reagent prepared by comparative example 1, comparative example 2, embodiment 1 to 3 is divided into 24 equal portions according to packing of product specification, places in 2-8 DEG C of refrigerator, monthly timing takes out one group, detects ammonia quality control product (target value is 61 μm of ol/L) according to operating process.
Operating process is: preincubate 3min after each 240 μ l Homogeneous phase mixing of reagent 1 in the reagent that (1) quality control product 20 μ l is prepared with comparative example, embodiment 1 to 3 respectively; (2) add reagent 2 in 40 μ l comparative examples, embodiment 1,2,3 respectively, postpone to hatch 5min; (3) blank tube zeroing, after 340nm measures absorbance A 1,2min, 340nm measures absorbance A 2, calculates △ A=A2-A1; (4) method of calculation are as follows:
Sample △ A
Sample ammonia density=------× normal concentration
Standard △ A
Detected result as shown in Figure 1.
As shown in Figure 1, after reagent places 2 years, embodiment 1 to 3 prepare reagent obviously than comparative example 1 and comparative example 2 more stable.
In ammonia detection reagent of the present invention, serum lactic dehydrogenase can except the pyruvic acid disturbing ammonia to measure in serum deprivation, γ-Fe
2o
3nanoparticle can strengthen the activity of serum lactic dehydrogenase, makes to react carry out more thorough, effectively removes the interference of pyruvic acid.Adenosine diphosphate (ADP) is the activator of enzyme reaction, effect weakens the combination of reduced coenzyme Ⅰ to zymophore, prevents the excessive suppression caused of coenzyme, therefore the adding of adenosine diphosphate (ADP), the stability of glutamate dehydrogenase in ealkaline buffer is increased, can also Reaction time shorten.Bovine serum albumin, as enzymatic protective reagent, can maintain the stability of enzyme in complex environment.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not by the restriction of embodiment; other is any do not deviate from spirit of the present invention and principle under make change, modification, combination, substitute, simplify and all should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (3)
1. a serum ammonia detection reagent, is characterized in that, it comprises the reagent 1 and reagent 2 that volume ratio is 6:1;
The component of described reagent 1 is:
PH is the tris damping fluid 10-50mmol/L of 8.0
Serum lactic dehydrogenase 2KU/L
Nanoparticle 10-50mmol/L
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol/L
Reduced coenzyme Ⅰ 0.2g/L
Bovine serum albumin 0.5-1g/L
Adenosine diphosphate (ADP) 0.1-0.6mmol/L
Deionized water adds to 1L;
Described nanoparticle is γ-Fe
2o
3nanoparticle, its particle diameter is 3-50nm;
The component of described reagent 2 is:
PH is the tris damping fluid 10-50mmol/L of 8.0
α-ketoglutaric acid 30mmol/L
Glutamate dehydrogenase 25KU/L
Bovine serum albumin 0.5-1g/L
Adenosine diphosphate (ADP) 0.1-0.6mmol/L
Deionized water adds to 1L.
2. ammonia detection reagent according to claim 1, is characterized in that, the component of described reagent 1 is:
PH is the tris damping fluid 30mmol/L of 8.0
Serum lactic dehydrogenase 2KU/L
Nanoparticle 25mmol/L
Ethylenediamine tetraacetic acid (EDTA) 5.3mmol/L
Reduced coenzyme Ⅰ 0.2g/L
Bovine serum albumin 0.8g/L
Adenosine diphosphate (ADP) 0.4mmol/L
Deionized water adds to 1L.
3. ammonia detection reagent according to claim 1, is characterized in that, the component of described reagent 2 is:
PH is the tris damping fluid 25mmol/L of 8.0
α-ketoglutaric acid 30mmol/L
Glutamate dehydrogenase 25KU/L
Bovine serum albumin 0.7g/L
Adenosine diphosphate (ADP) 0.4mmol/L
Deionized water adds to 1L.
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|---|---|---|---|
| CN201410697748.1A CN104374906B (en) | 2014-11-28 | 2014-11-28 | A kind of serum ammonia detection reagent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410697748.1A CN104374906B (en) | 2014-11-28 | 2014-11-28 | A kind of serum ammonia detection reagent |
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| CN104374906B true CN104374906B (en) | 2016-02-17 |
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ID=52553951
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| CN106811505B (en) * | 2015-11-30 | 2020-11-24 | 山东博科生物产业有限公司 | Single-reagent liquid ammonia blood (AMM) detection reagent with strong stability |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2329174C2 (en) * | 1973-06-07 | 1975-07-24 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method for the quantitative determination of blood ammonia |
| US5141853A (en) * | 1988-07-22 | 1992-08-25 | Abbott Laboratories | Determination of ammonia levels in a sample |
| CN1256442C (en) * | 2004-09-27 | 2006-05-17 | 哈尔滨医科大学 | Blood ammonia reagent kit for enzyme detection |
| CN1778963A (en) * | 2004-11-23 | 2006-05-31 | 苏州艾杰生物科技有限公司 | Determination of blood ammonia content and blood ammonia diagnostic reagent kit |
| CN1778946A (en) * | 2004-11-23 | 2006-05-31 | 苏州艾杰生物科技有限公司 | Determination of blood ammonia content and blood ammonia diagnostic reagent kit |
| CN103543117A (en) * | 2013-10-21 | 2014-01-29 | 大连市沙河口区中小微企业服务中心 | Measurement method of ammonia content in serum |
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2014
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