CN104374856A - Method for analysis determination of glycyrrhizic acid content - Google Patents
Method for analysis determination of glycyrrhizic acid content Download PDFInfo
- Publication number
- CN104374856A CN104374856A CN201410471055.0A CN201410471055A CN104374856A CN 104374856 A CN104374856 A CN 104374856A CN 201410471055 A CN201410471055 A CN 201410471055A CN 104374856 A CN104374856 A CN 104374856A
- Authority
- CN
- China
- Prior art keywords
- glycyrrhizic acid
- mobile phase
- liquid phase
- carrying
- efficient liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention relates to a method for analysis determination of the glycyrrhizic acid content. The method comprises: 1) precisely weighing 24 mg of a glycyrrhizic acid pure product, adding into a 50 mL volumetric flask, adding a mobile phase to dissolve, carrying out volume metering to achieve the mark, uniformly shaking, respectively and precisely sucking 0.5 mL, 1.5 mL, 2.5 mL, 3.75 mL and 6.25 mL of the solution into 10 mL volumetric flasks, adding a mobile phase to dissolve, carrying out volume metering to achieve the mark, carrying out high-performance liquid chromatography analysis according to chromatographic conditions, carrying out regression analysis by adopting the concentration on the peak area to obtain a glycyrrhizic acid standard curve, and calculating the regression equation; and 2), precisely weighing 0.01 g of a glycyrrhizic acid sample, adding a mobile phase, carrying out volume metering to achieve 25 ml, filtering with a 0.22 [mu]m microporous filtration membrane, carrying out high-performance liquid chromatography analysis according to chromatographic conditions, and substituting the peak area into the glycyrrhizic acid standard curve so as to calculate the glycyrrhizic acid content. With the method of the present invention, the more favorable basis is provided for further research and development of the glycyrrhizic acid.
Description
Technical field
The present invention relates to a kind of analyzing detecting method, relate to a kind of method that efficient liquid phase chromatographic analysis measures glycyrrhizic acid content.
Background technology:
Glycyrrhizic acid is a kind of natural sweetener extracted in the root of pulse family (legumrrihiza) Glycyrrhiza (glycyrrhiza) plant or rhizome, has another name called glycyrrhizic acid element, Radix Glycyrrhizae soap former times.Its feature is high sugariness (its sugariness is 200-300 times of sucrose), low calorie, safety non-toxic, and foamability and haemocylolysis are very low, can be widely used in the industries such as food, beverage, medicine, cosmetics, tobacco.Therefore, people are enjoyed to pay close attention to about the research of licorice glycyrrhizic acid in recent years.The present invention is intended to set up analyze and measures the method for glycyrrhizic acid content, for further investigation provides more how favourable foundation with these superior resources of exploitation further, and provides fundamental basis for the exploitation of low calorie sweetener.
Summary of the invention
The object of the invention is to set up a kind of method that efficient liquid phase chromatographic analysis measures glycyrrhizic acid content.
The technical solution used in the present invention is:
Efficient liquid phase chromatographic analysis measures a method for glycyrrhizic acid content, comprises the steps:
1): the drafting of typical curve
Precision takes in glycyrrhizic acid sterling 24mg to 50mL volumetric flask, adds mobile phase and dissolves and be settled to scale, shake up; Respectively in accurate draw solution 0.5mL, 1.5mL, 2.5mL, 3.75mL, 6.25mL to 10mL volumetric flask, mobile phase dissolves and is settled to scale; Efficient liquid phase chromatographic analysis is carried out by chromatographic condition; With concentration, regretional analysis is carried out to peak area, obtain glycyrrhizic acid typical curve, calculate regression equation;
2), sample pre-treatments:
Precision takes glycyrrhizic acid sample 0.01g, adds mobile phase and is settled to 25ml, with the filtering with microporous membrane of 0.22 μm, carries out efficient liquid phase chromatographic analysis by chromatographic condition; Peak area is brought into glycyrrhizic acid typical curve, can glycyrrhizic acid content be calculated.
Described efficient liquid phase chromatographic analysis condition is:
Chromatographic condition: column type: Kromasil C
18post (150 × 4.6mm ID, 5 μm);
Mobile phase: methyl alcohol-0.2mol/L ammonium acetate solution-glacial acetic acid (66:33:1)
Flow velocity: 0.8mL/min, column temperature: 25 DEG C, sample size: 20.0 μ L
Determined wavelength: 250nm.
The beneficial effect that the present invention has:
The present invention carries out content analysis by high-efficient liquid phase analysis to glycyrrhizic acid, provides solid reference for extracting this sweet substance of glycyrrhizic acid from Radix Glycyrrhizae.For glycyrrhizic acid purity in later licorice, extraction ratio etc. provide computing method.
In general experiment, the comprehensive purity of glycyrrhizic acid in extraction ratio and crude product that adopts evaluates extraction process.
M
1glycyrrhizic acid quality in-glycyrrhizic acid inclusion compound
M
0glycyrrhizic acid gross mass in-Radix Glycyrrhizae powder
M
lglycyrrhizic acid quality in-glycyrrhizic acid inclusion compound
M
2-glycyrrhizic acid meal quality.
Accompanying drawing explanation
Fig. 1 is the high-efficient liquid phase chromatogram of glycyrrhizic acid sterling.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, but do not limit protection scope of the present invention.
Efficient liquid phase chromatographic analysis measures a method for glycyrrhizic acid content, comprises the steps:
1): the drafting of typical curve
Precision takes in glycyrrhizic acid sterling 24mg to 50mL volumetric flask, adds mobile phase and dissolves and be settled to scale, shake up; Respectively in accurate draw solution 0.5mL, 1.5mL, 2.5mL, 3.75mL, 6.25mL to 10mL volumetric flask, mobile phase dissolves and is settled to scale; Efficient liquid phase chromatographic analysis is carried out by chromatographic condition; With concentration, regretional analysis is carried out to peak area, obtain glycyrrhizic acid typical curve, calculate regression equation;
2), sample pre-treatments:
Precision takes glycyrrhizic acid sample 0.01g, adds mobile phase and is settled to 25ml, with the filtering with microporous membrane of 0.22 μm, carries out efficient liquid phase chromatographic analysis by chromatographic condition; Peak area is brought into glycyrrhizic acid typical curve, can glycyrrhizic acid content be calculated.
Described efficient liquid phase chromatographic analysis condition is:
Chromatographic condition: column type: Kromasil C
18post (150 × 4.6mm ID, 5 μm);
Mobile phase: methyl alcohol-0.2mol/L ammonium acetate solution-glacial acetic acid (66:33:1)
Flow velocity: 0.8mL/min, column temperature: 25 DEG C, sample size: 20.0 μ L
Determined wavelength: 250nm.
Claims (2)
1. efficient liquid phase chromatographic analysis measures a method for glycyrrhizic acid content, it is characterized in that: comprise the steps:
1): the drafting of typical curve
Precision takes in glycyrrhizic acid sterling 24mg to 50mL volumetric flask, adds mobile phase and dissolves and be settled to scale, shake up; Respectively in accurate draw solution 0.5mL, 1.5mL, 2.5mL, 3.75mL, 6.25mL to 10mL volumetric flask, mobile phase dissolves and is settled to scale; Efficient liquid phase chromatographic analysis is carried out by chromatographic condition; With concentration, regretional analysis is carried out to peak area, obtain glycyrrhizic acid typical curve, calculate regression equation;
2), sample pre-treatments:
Precision takes glycyrrhizic acid sample 0.01g, adds mobile phase and is settled to 25ml, with the filtering with microporous membrane of 0.22 μm, carries out efficient liquid phase chromatographic analysis by chromatographic condition; Peak area is brought into glycyrrhizic acid typical curve, can glycyrrhizic acid content be calculated.
2. a kind of efficient liquid phase chromatographic analysis measures the method for glycyrrhizic acid content according to claim 1, it is characterized in that: described efficient liquid phase chromatographic analysis condition is:
Chromatographic condition: column type: Kromasil C
18post (150 × 4.6mm ID, 5 μm);
Mobile phase: methyl alcohol-0.2mol/L ammonium acetate solution-glacial acetic acid (66:33:1)
Flow velocity: 0.8mL/min, column temperature: 25 DEG C, sample size: 20.0 μ L
Determined wavelength: 250nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410471055.0A CN104374856A (en) | 2014-09-16 | 2014-09-16 | Method for analysis determination of glycyrrhizic acid content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410471055.0A CN104374856A (en) | 2014-09-16 | 2014-09-16 | Method for analysis determination of glycyrrhizic acid content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104374856A true CN104374856A (en) | 2015-02-25 |
Family
ID=52553901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410471055.0A Pending CN104374856A (en) | 2014-09-16 | 2014-09-16 | Method for analysis determination of glycyrrhizic acid content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104374856A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248830A (en) * | 2016-08-24 | 2016-12-21 | 江苏天晟药业股份有限公司 | A kind of detection method of Radix Glycyrrhizae acid content |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070065749A1 (en) * | 2005-09-21 | 2007-03-22 | Vladek Kasperchik | Radiation-markable coatings for printing and imaging |
-
2014
- 2014-09-16 CN CN201410471055.0A patent/CN104374856A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070065749A1 (en) * | 2005-09-21 | 2007-03-22 | Vladek Kasperchik | Radiation-markable coatings for printing and imaging |
Non-Patent Citations (4)
| Title |
|---|
| 李仁秋: "高效液相色谱法测定新止咳合剂中甘草酸的含量", 《云南中医中药杂志》 * |
| 王玫等: "HPLC法测定宁神灵颗粒中的甘草酸含量", 《黑龙江医药》 * |
| 石嫱等: "八味檀香散中甘草酸的含量测定", 《医药导报》 * |
| 黄丽丹: "HPLC法测定脑力静糖浆中甘草酸的含量", 《安徽医药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248830A (en) * | 2016-08-24 | 2016-12-21 | 江苏天晟药业股份有限公司 | A kind of detection method of Radix Glycyrrhizae acid content |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101961430B (en) | Quality analysis method of compound Ganmaoling tablets | |
| CN105294628A (en) | Method for preparing flavonoid component by separating wild chrysanthemum flower | |
| CN103852531B (en) | Method for detecting malto-oligosaccharide in beer through HPLC-ELSD (High-Performance Liquid Chromatography-Evaporative Light Scattering Detector) | |
| CN103340938A (en) | Preparation method for tartary buckwheat standard extract FT83 and high pure quercetin | |
| CN103698422B (en) | Method for detecting contents of baicalin, forsythin, indirubin and glycyrrhizic acid in Qingreling granules | |
| CN101810696B (en) | Total triterpenoids of apples and method for preparing same | |
| CN103592391A (en) | Method for determining specnuezhenide content in Zhenqifuzheng preparation | |
| CN104374856A (en) | Method for analysis determination of glycyrrhizic acid content | |
| CN105294722A (en) | Preparation method for high-purity glabridin monomer | |
| CN108020613B (en) | Method for measuring content of menthol in traditional Chinese medicine composition | |
| CN101195646A (en) | Production method of stilbene glycoside extract | |
| CN102532077B (en) | Method for preparing salvianolic acid B through separation by means of flash chromatography | |
| CN101975826B (en) | Method for measuring active ingredients of Chinese herbal medicine rhubarb additive in cigarettes and cigarette mainstream smoke | |
| CN104725455B (en) | Preparation method of ganoderic acid T | |
| CN105859715A (en) | Critical fluid chromatographic method for separating and purifying evodiamine and rutaecarpine from fructus evodiae | |
| CN103424499B (en) | Method for detecting picrasma quassioides alkali content in picrasma quassioides | |
| CN103524342B (en) | Adopt the method for adjustable ternary solvent system sharp separation tussilagone | |
| CN102706984A (en) | Method for determining ephedrine hydrochloride content in lung-clearing inflammation pill by high-performance liquid phase | |
| CN104892708A (en) | Method for scale preparation of adenosine chemical reference substance from armillaria luteo-virens fruiting body | |
| CN103275054A (en) | Method for preparing bellidifolin | |
| CN103323551A (en) | Method for detecting content of medlar acid | |
| CN104678021B (en) | A kind of method of 3 kinds of diester-type diterpene alkaloid class materials in yellow Radix Aconiti Kusnezoffii of mensuration simultaneously | |
| CN1994995A (en) | Method for extracting and purifying sequoyitol | |
| CN103499655A (en) | Detection method of vanillin in wine | |
| CN103033584B (en) | Judgment method for quickly distinguishing two similar medicinal materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150225 |