CN104374776B - Method for rapidly detecting traces of chloroform - Google Patents
Method for rapidly detecting traces of chloroform Download PDFInfo
- Publication number
- CN104374776B CN104374776B CN201410748380.7A CN201410748380A CN104374776B CN 104374776 B CN104374776 B CN 104374776B CN 201410748380 A CN201410748380 A CN 201410748380A CN 104374776 B CN104374776 B CN 104374776B
- Authority
- CN
- China
- Prior art keywords
- chloroform
- gold nano
- nano grain
- hydrosol
- minim
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a detection method, in particular to a method for rapidly detecting traces of chloroform. The method includes the steps of firstly preparing gold nanoparticle hydrosol and then detecting traces of the chloroform through the gold nanoparticle hydrosol. The gold nanoparticle hydrosol is successfully prepared with oxyethylene steroid (BPS-30) as surfactant; the prepared gold nanoparticle hydrosol is used for detecting chloroform residue; qualitative analysis is conducted through visual observation or a UV (ultraviolet) spectrophotometer. With the gold nanoparticle hydrosol coated by the surfactant of sterols used, colloids are strong in stability and easy to carry. When the gold nanoparticle hydrosol meets the chloroform, the gold nanoparticle hydrosol immediately coagulates to make sol change from purple to light gray; within five minutes, the gold nanoparticle hydrosol completely coagulates, the sol is separated, and transparent aqueous solution is on the upper layer, while aggregation of black gold nanoparticles is on the lower layer. The said changes can be visually detected and determined. Furthermore, operation is simple, and efficiency is high.
Description
Technical field
The present invention relates to a kind of detection method is and in particular to a kind of method of quick detection minim chloroform.
Background technology
Chloroform is very harmful to human body, and chloroform has moderate toxicity, can connect through alimentary canal, respiratory tract, skin
Touch into entering body.It is a kind of strong cardiovascular g of formulation, also toxic to liver, kidney, is likely to result in the enlargement of liver
And necrosis.Meanwhile, its Central nervous system can produce restraint effect.Its important Acute Toxicity is Central nervous system
Have anesthetic effect, have stimulation to eye and skin, and heart, liver, kidney can be encroached on, in addition can degreasing, severe patient is even
Can be fatal.
At present, prior art mainly realizes the separation to chloroform and detection, test behaviour by gas chromatograph
Make complicated, test environment has high demands, high cost is not suitable for being applied to the field condition of complex condition.Such as publication No. CN
A kind of employing gas-chromatography (GC) method of the patent disclosure of 103543231 A, electron capture detector (ECD) is beautiful to detect hydrochloric acid
The residual quantity of chloroform in clo piperazine, its detailed process includes solution and the preparation of comparison liquid and instrument detects, instrument detection bag
Containing instruments such as gas chromatograph, electron capture detectors, higher to detected sample requirement condition, instrument accuracy grade, noise ratio
And stability parameter etc. all can affect the accuracy of testing result.Furthermore, instrument is generally fixed to ad-hoc location it is impossible to carry out
Mobile, field test etc. is required also cannot meet.As can be seen here, it is badly in need of a kind of simple and convenient, process of research and development easily and fast
A kind of detection minim chloroform method of identification.
Content of the invention
The technical problem to be solved in the present invention is how to overcome the deficiencies in the prior art, provides a kind of quick detection Micro Amounts of Chlorine
Imitative method.The hydrosol of the gold nano grain being coated using sterols surfactant in the present invention, colloidal stability is strong, just
In carrying, can carry out observing determination, simple to operate, efficiency high by naked eyes.
The technical solution of the present invention is a kind of method of quick detection minim chloroform, comprises the following steps：The first step,
The preparation of the gold nano grain hydrosol；Second step, the gold nano grain hydrosol detects minim chloroform.
Further, the preparation process of the described gold nano grain hydrosol is as follows：
Under room temperature condition, by 0.5～3mL sterols surfactant (75mmol/L) aqueous solution and 0.05～0.3mL
HAuCl4The aqueous solution (10mmol/L) is added in 0.85mL water, is sufficiently mixed；Ensure BPS-30 and HAuCl4Ultimate density divide
Wei not 40～60mmol/L and 0.1～1mmol/L；Mixed solution stands 8～24h, centrifugation, and deionized water fully washs sample
Product, to no reagent residual；The gold nano grain finally giving is added in 5mL water and can get the gold nano grain hydrosol.
Further, described sterols surfactant is preferably oxyethylene group steroid (BPS-30).
Further, the stability of the gold nano grain hydrosol can be detected by uv-visible absorption spectra.
Further, described gold nano grain hydrosol detection minim chloroform step is as follows：
The chloroform of certain system is added dropwise in the above-mentioned gold nano grain hydrosol, stands 1～10min after mixing, pass through
In uv-visible absorption spectra, the peak intensity change of gold nano grain or naked eyes are watched and to be detected the coagulation feelings of gold nano grain
Condition, identification chloroform whether there is.
Further, uv-visible absorption spectra detects gold nano grain absworption peak in 520～550nm.
Further, the coagulation situation of detection gold nano grain can also pass through transmission electron microscope TEM or scanning electron microscope sem.
Further, the gold nano grain hydrosol has aubergine to be changed into light gray and finally produce black precipitate, determines
Chloroform exists；By transmission electron microscope TEM or scanning electron microscope sem, gold nano grain hydrosol aggregate and precipitate determines that chloroform exists；Logical
Cross uv-visible absorption spectra detection gold nano grain, gold nano grain has the determination chloroform that dies down by force to deposit at 520～550nm peak
?.
Further, described centrifugal rotational speed is 5000r/min.
Further, the chloroform adding 5～20 μ L in the 5mL gold nano grain hydrosol can be detected really by ultraviolet spectra
Fixed, add chloroform volume can determine by naked-eye observation more than 20 μ L.
Beneficial effects of the present invention are as follows：
The hydrosol of the gold nano grain being coated using sterols surfactant in the present invention, colloidal stability is strong, just
In carrying.After gold nano colloidal sol runs into chloroform, gold nano grain coagulation immediately, make colloidal sol color be changed into light ash from aubergine
Color, within 5 minutes, to test tube bottom, being separated occurs the complete coagulation of gold nano grain in colloidal sol, and upper strata is changed into the transparent aqueous solution,
Lower floor is black gold nano grain aggregation, and above-mentioned change can carry out observing determination by naked eyes completely, simple to operate, effect
Rate is high.
Brief description
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is oxyethylene group steroid (BPS-30) chemical constitution schematic diagram of the present invention；
Fig. 2 is gold nano grain TEM figure of the present invention；
Fig. 3 is the gold nano grain hydrosol UV-visible absorption spectrum of present invention difference standing time；
Fig. 4 is the gold nano grain peak intensity variation diagram of different volumes chloroform of the present invention；
Fig. 5 is the gold nano grain hydrosol photo of different volumes chloroform of the present invention；
Fig. 6 is that gold nano grain of the present invention assembles TEM figure；
Fig. 7 is the gold nano grain peak intensity variation diagram of present invention difference organic reagent；
Fig. 8 is the gold nano grain hydrosol photo of present invention difference organic reagent；
Fig. 9 is the gold nano grain hydrosol photo before and after the present invention adds mixed organic solvents；
Specific embodiment
Referring to embodiment, the method describing a kind of present invention quick detection minim chloroform in detail.
The coagulation principle of gold nano grain is to lose cladding ability due to covering oxyethylene group steroid (BPS-30)
Cause.In the gold nano grain hydrosol, due to the presence of oxyethylene group steroid (BPS-30), around gold nano grain
Form layer protective layer, prevent particle coagulation.After chloroform adds, system occurs in that water-oil phase, creates profit circle in large quantities
Face, oxyethylene group steroid (BPS-30) easily holds absorption in oil-water interfaces as typical nonionic surfactant, thus losing
Go the cladding ability for gold nano grain, nano Au particle surface charge produces change and leads to mutually assemble and precipitate.
It comprises the following steps that：
(1) preparation of the gold nano grain hydrosol and stability test：
1.1 experiment reagent：Oxyethylene group steroid (BPS-30 analyzes pure, Japanese daylight company)；Gold chloride
(HAuCl4·4H2O, analyzes pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Water is deionized water.
1.2 laboratory apparatus：U-4100 type ultraviolet-visible spectrophotometer (HITACHI company)；Transmission electron microscope
TEM(JEM-100CX II(JEOL).
The synthesis of 1.3 gold nano grains：Under room temperature condition, will be water-soluble for 2.0mL sterols surfactant (75mmol/L)
Liquid and 0.15mL HAuCl4The aqueous solution (10mmol/L) is added in 0.85mL water, is sufficiently mixed；Ensure BPS-30 and HAuCl4
Ultimate density be respectively 50mmol/L and 0.5mmol/L；Mixed solution stands 12h, centrifugation, and deionized water fully washs sample
Product, to reactionless reagent residual；The gold nano grain finally giving is added in 5mL water to can get gold nano grain water-soluble
Glue.BPS-30 structural formula, as shown in figure 1, carrying hydrophilic radical and lipophilic group, is surfactant.The gold nano grain hydrosol
As shown in Fig. 2 gold nano grain is divided equally disperseing, particle size is homogeneous, has good stability.
1.4 gold nano grain Stability of colloidal dispersions：Detect gold nano grain 6 respectively by uv-visible absorption spectra
Hour, 1 day, 5 days, 10 days, 30 days peak intensities in 520～550nm.
As shown in figure 3, the peak intensity result by gold nano grain peak (520～550nm) in uv-visible absorption spectra
Display, the gold nano grain hydrosol remains in that peak intensity is basically unchanged after preparing 30 days, and result is the gold nano grain hydrosol
Have good stability.
(2) the gold nano grain hydrosol is used for the detection of minim chloroform
2.1 experiment reagent：Chloroform (CH3Cl, analyzes pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Water is deionized water.
2.2 laboratory apparatus：U-4100 type ultraviolet-visible spectrophotometer (HITACHI company)；Transmission electron microscope
TEM(JEM-100CX II(JEOL)；Canon's digital camera (EOS-M).
The detection of 2.3 minim chloroforms：Respectively the chloroform of 0uL, 10uL, 20uL, 30uL, 40uL, 50uL is added dropwise to above-mentioned
The gold nano grain hydrosol having configured, stands 5min after mixing, by gold nano grain peak in uv-visible absorption spectra
The peak intensity change of (520～550nm) and common photo are determining the coagulation situation of gold nano grain.
As shown in figure 4,10uL group compares blank control group 0uL, gold nano grain peak area reduces, and illustrates depositing of chloroform
?.Absworption peak at gold nano grain 520～550nm for 20uL, 30uL, 40uL, 50uL group disappears, and the chloroform content of explanation increases
Plus, also demonstrate chloroform residual simultaneously.
As shown in figure 5, the aubergine color of 10uL, 20uL, 30uL, 40uL, 50uL group sample gradually thin out to disappearing completely
Lose, compared with blank control group 0uL, 50uL group shows substantially.
Result above can also determine that the chloroform adding 10 μ L in the 5mL gold nano grain hydrosol both can pass through purple simultaneously
External spectrum detection determines, adds chloroform volume determine can more than 20 μ L and by naked-eye observation.
Fig. 6 adds the photo after chloroform for gold nano grain, and coding is consistent with Fig. 5 coding, and Jenner can be clearly seen
Rice grain there occurs obvious gathering in the solution exist chloroform, and almost all of nano-particle all flocks together.
(3) the exclusiveness inspection of other organic reagents
3.1 experiment reagent：Chloroform (CH3Cl, analyzes pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Methyl alcohol (CH3OH, point
Analyse pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Oxolane (C4H8O, analyzes pure, the limited public affairs of Chinese medicines group chemical reagent
Department)；Acetone (C3H6O, analyzes pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Acetonitrile (C2H3N, analysis is pure, Chinese medicines group chemistry
Reagent Co., Ltd)；Dimethylformamide (C3H7NO, analyzes pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Dimethyl sulfoxide (DMSO)
(C2H6OS, analyzes pure, Chemical Reagent Co., Ltd., Sinopharm Group)；Acetic acid (C2H5COOH, analyzes pure, Chinese medicines group chemical reagent
Co., Ltd)；Water is deionized water.
3.2 laboratory apparatus：U-4100 type ultraviolet-visible spectrophotometer (HITACHI company)；Canon's digital camera
(EOS-M).
3.3 experimentation：Organic reagent (methyl alcohol, oxolane, acetone, acetonitrile, dimethyl formyl from 7 quasi-representatives
Amine, dimethyl sulfoxide (DMSO), acetic acid) compare experiment with chloroform.50 μ L chloroforms are added respectively in the 5mL gold nano grain hydrosol
And methyl alcohol, oxolane, acetone, acetonitrile, dimethylformamide, dimethyl sulfoxide (DMSO), acetic acid organic reagent.Stand after mixing
5min, by peak intensity change (Fig. 7) and the common photograph of gold nano grain peak (520～550nm) in uv-visible absorption spectra
Piece (Fig. 8) is determining the coagulation situation of gold nano grain.
3.4 experimental result：As shown in fig. 7, methyl alcohol, oxolane, acetone, acetonitrile, dimethylformamide, dimethyl are sub-
After sulfone, acetic acid organic reagent add gold nano grain, gold nano grain is unchanged in the peak intensity of 520～550nm, in chloroform
Middle addition gold nano grain, gold nano grain disappears at the peak of 520～550nm.For further illustrating, as shown in figure 8, methyl alcohol,
Oxolane, acetone, acetonitrile, dimethylformamide, dimethyl sulfoxide (DMSO), acetic acid organic reagent coding are consistent with Fig. 7 coding, plus
After after entering gold nano grain, sample still shows aubergine, and chloroform reagent adds gold nano grain, occur in that aubergine disappears
Lose, bottom occurs in that the sediment of black simultaneously, above all illustrate only chloroform that gold nano grain can be made coagulation is existing
As.
3.5 tests are follow-up：In order to prove to detect the effect of chloroform in mixed organic solvents further, above-mentioned 8 kinds are had
Machine reagent is obtained mixed organic solvents after pressing equal-volume mixing.Take 400 μ L mixed organic solvents (wherein chloroform has 50 μ L), add
The gold nano grain hydrosol, as experimental group.Control group is the gold nano grain hydrosol.(Fig. 9) is observed by common photo, right
According to group 1 compared with experimental group 2, experimental group 2 occurs in that obvious precipitation, even if illustrating to use mixed organic solvents, wherein deposits
Chloroform remain to using inventor method naked-eye observation determine.
The above, be only embodiments of the invention, and not the present invention is made with any pro forma restriction, any ripe
Know those skilled in the art, without departing under technical solution of the present invention ambit, using the method content pair of the disclosure above
Technical solution of the present invention makes many possible variations and modification, belongs to the scope of claims protection.
Claims (10)
1. a kind of method of quick detection minim chloroform it is characterised in that：Comprise the following steps：The first step, gold nano grain water
The preparation of colloidal sol；Second step, the gold nano grain hydrosol detects minim chloroform, and the described gold nano grain hydrosol is by sterols
Surfactant and HAuCl4Prepared by the aqueous solution.
2. a kind of quick detection minim chloroform according to claim 1 method it is characterised in that：Gold nano grain is water-soluble
The preparation process of glue is as follows：
Under room temperature condition, by 0.5～3mL concentration be the oxyethylene group steroid aqueous solution of 75mmol/L and 0.05～0.3mL is dense
Spend the HAuCl for 10mmol/L4The aqueous solution is added in 0.85mL water, is sufficiently mixed；Ensure oxyethylene group steroid and HAuCl4
Ultimate density be respectively 40～60mmol/L and 0.1～1mmol/L；Mixed solution stands 8～24h, centrifugation, deionized water
Fully washing sample, to no reagent residual；The gold nano grain finally giving is added in 5mL water and can get gold nano
The grain hydrosol.
3. a kind of quick detection minim chloroform according to claim 2 method it is characterised in that：Also include by purple
Outward-visible absorption spectra detects the stability of the gold nano grain hydrosol.
4. a kind of quick detection minim chloroform according to claim 1 method it is characterised in that：Described gold nano grain
Hydrosol detection minim chloroform step is as follows：
Detection liquid is added dropwise in the above-mentioned gold nano grain hydrosol, stands 1～10min after mixing, by ultraviolet-ray visible absorbing
In spectrum, whether the peak intensity change of gold nano grain or naked eyes viewing, to detect the coagulation situation of gold nano grain, identify chloroform
Exist.
5. a kind of quick detection minim chloroform according to claim 3 or 4 method it is characterised in that：Ultraviolet-visible is inhaled
Receive spectrum and detect gold nano grain in 520～550nm.
6. a kind of quick detection minim chloroform according to claim 4 method it is characterised in that：Watch gold by naked eyes
The nano particle hydrosol is changed into light gray by aubergine and finally produces black precipitate, determines that chloroform exists.
7. a kind of quick detection minim chloroform according to claim 4 method it is characterised in that：By transmission electron microscope
TEM or scanning electron microscope sem, gold nano grain hydrosol aggregate and precipitate determines that chloroform exists.
8. a kind of quick detection minim chloroform according to claim 4 method it is characterised in that：By ultraviolet-visible
Absorption spectrum detects gold nano grain, by dying down by force, gold nano grain determines that chloroform exists at 520～550nm peak.
9. a kind of quick detection minim chloroform according to claim 2 method it is characterised in that：Centrifugal rotational speed >=
5000r/min.
10. a kind of quick detection minim chloroform according to claim 1 or 4 method it is characterised in that：In 5mL Jenner
The rice grain hydrosol adds the chloroform of 5～20 μ L can detect determination by ultraviolet spectra, and addition chloroform volume more than 20 μ L is
Can be determined by naked-eye observation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410748380.7A CN104374776B (en) | 2014-12-09 | 2014-12-09 | Method for rapidly detecting traces of chloroform |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410748380.7A CN104374776B (en) | 2014-12-09 | 2014-12-09 | Method for rapidly detecting traces of chloroform |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104374776A CN104374776A (en) | 2015-02-25 |
| CN104374776B true CN104374776B (en) | 2017-02-22 |
Family
ID=52553823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410748380.7A Expired - Fee Related CN104374776B (en) | 2014-12-09 | 2014-12-09 | Method for rapidly detecting traces of chloroform |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104374776B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107727596B (en) * | 2017-09-30 | 2019-11-08 | 中国石油大学(华东) | A method of quickly detecting micro mercury ion |
| CN111122766A (en) * | 2018-10-31 | 2020-05-08 | Tcl集团股份有限公司 | Method for detecting chloroform in sulfur-containing quantum dot solution |
| CN111122556B (en) * | 2018-10-31 | 2021-07-16 | Tcl科技集团股份有限公司 | Method for detecting chloroform in quantum dot solution |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101943692A (en) * | 2010-08-11 | 2011-01-12 | 上海师范大学 | Air-sensitive material with high-sensitivity and quick-response, and preparation method and application thereof |
| KR20120094872A (en) * | 2011-02-17 | 2012-08-27 | 이화여자대학교 산학협력단 | Producing method of au nanoparticle pattern having localized surface plasmon resonance coupling property, and au nanoparticle pattern by the same |
| CN102675800A (en) * | 2012-01-04 | 2012-09-19 | 河南科技大学 | Preparation method of ionic liquid-gold nano particle composite hydrogel with anion responsivity |
-
2014
- 2014-12-09 CN CN201410748380.7A patent/CN104374776B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101943692A (en) * | 2010-08-11 | 2011-01-12 | 上海师范大学 | Air-sensitive material with high-sensitivity and quick-response, and preparation method and application thereof |
| KR20120094872A (en) * | 2011-02-17 | 2012-08-27 | 이화여자대학교 산학협력단 | Producing method of au nanoparticle pattern having localized surface plasmon resonance coupling property, and au nanoparticle pattern by the same |
| CN102675800A (en) * | 2012-01-04 | 2012-09-19 | 河南科技大学 | Preparation method of ionic liquid-gold nano particle composite hydrogel with anion responsivity |
Non-Patent Citations (5)
| Title |
|---|
| Aggregation-Resistant Water-Soluble Gold Nanoparticles;Layal L. Rouhana et al.;《Langmuir》;20071116;第23卷(第26期);第12799-12801页 * |
| Controllable Synthesis of Water-Soluble Gold Nanoparticles and Their Applications in Electrocatalysis and Surface-Enhanced Raman Scattering;Yan Qiao et al.;《Langmuir》;20110618;第27卷(第17期);第11090-11097页 * |
| 憎水性金属纳米粒子及其LB膜的制备与光谱表征;黄薇等;《光谱学与光谱分析》;20000625;第20卷(第3期);第449-452页 * |
| 纳米金比色法测定大分子分子量;凌凯;《中国博士学位论文全文数据库工程科技I辑》;20141115(第11期);摘要第1页 * |
| 表面活性剂辅助制备金纳米材料;贾寒;《中国博士论文全文数据库工程科技I辑》;20130915(第9期);正文第103页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104374776A (en) | 2015-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104374776B (en) | Method for rapidly detecting traces of chloroform | |
| Yang et al. | On-site, rapid and visual determination of Hg2+ and Cu2+ in red wine by ratiometric fluorescence sensor of metal-organic frameworks and CdTe QDs | |
| Wang et al. | An europium functionalized carbon dot-based fluorescence test paper for visual and quantitative point-of-care testing of anthrax biomarker | |
| Wang et al. | Sensitive and selective colorimetric detection of cadmium (II) using gold nanoparticles modified with 4-amino-3-hydrazino-5-mercapto-1, 2, 4-triazole | |
| Huang et al. | Tyndall-effect-enhanced supersensitive naked-eye determination of mercury (II) ions with silver nanoparticles | |
| Guan et al. | Direct colorimetric biosensing of mercury (II) ion based on aggregation of poly-(γ-glutamic acid)-functionalized gold nanoparticles | |
| Khataee et al. | Flow-injection chemiluminescence analysis for sensitive determination of atenolol using cadmium sulfide quantum dots | |
| Lin et al. | Dual-emission ratiometric fluorescence probe based on copper nanoclusters for the detection of rutin and picric acid | |
| Ljungkvist et al. | Two techniques to sample non-volatiles in breath—exemplified by methadone | |
| CN106832296A (en) | A kind of preparation method and application of saxitoxin molecular engram nano-luminescent material | |
| Zhao et al. | Rapid Detection of Sildenafil Drugs in Liquid Nutraceuticals Based on Surface‐Enhanced Raman Spectroscopy Technology | |
| US20200041418A1 (en) | Nanoplasmonic paper substrate for identification of fentanyl and fentanyl-related compounds | |
| Qi et al. | A highly sensitive SERS sensor for quantitative analysis of glucose based on the chemical etching of silver nanoparticles | |
| CN103091314A (en) | Rapid detection method for water-soluble azo dyes in foods | |
| Li et al. | Highly sensitive detection of dopamine based on gold nanoflowers enhanced-Tb (III) fluorescence | |
| Teng et al. | Determination of prostate cancer marker Zn2+ with a highly selective surface-enhanced Raman scattering probe on liquid–liquid self-assembled Au nanoarrays | |
| Sha et al. | A rapid detection method for on-site screening of estazolam in beverages with Au@ Ag core-shell nanoparticles paper-based SERS substrate | |
| CN104764706B (en) | Based on Au Fe3O4The double mode sensor of melamine of composite nanoparticle and preparation | |
| Wen et al. | Matrix colorimetry for high-resolution visual detection of free cyanide with Au@ Au–Ag yolk–Shell nanoparticles | |
| CN110280755A (en) | A kind of gold nano-probe and its preparation method and application for detecting benzoyl peroxide | |
| Xu et al. | A simple synthesis method of microsphere immunochromatographic test strip for time-resolved luminescence detection of folic acid | |
| CN104330364A (en) | Colorimetric detection method of iodide ions | |
| CN106124477B (en) | The detection method of concentration of silver ions and rate is discharged in a kind of nano silver course of dissolution | |
| Mekprayoon et al. | Performance evaluation of flow field-flow fractionation and electrothermal atomic absorption spectrometry for size characterization of gold nanoparticles | |
| Xu et al. | Sensitive and selective detection of mercury (II) based on the aggregation of gold nanoparticles stabilized by riboflavin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170222 Termination date: 20181209 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |