CN104371350A - Method for extracting and separating symbiotic pigment of red sorghum starch - Google Patents
Method for extracting and separating symbiotic pigment of red sorghum starch Download PDFInfo
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- CN104371350A CN104371350A CN201410569824.0A CN201410569824A CN104371350A CN 104371350 A CN104371350 A CN 104371350A CN 201410569824 A CN201410569824 A CN 201410569824A CN 104371350 A CN104371350 A CN 104371350A
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- sorghum starch
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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Abstract
The invention relates to a method for extracting and separating a symbiotic pigment of red sorghum starch, belonging to the field of purification and preparation methods of foods. The method comprises the following steps: cleaning and drying sorghum seeds, sieving by a 60mesh sieve, adding an extracting solution liquid, extracting, filtering, concentrating the extracting solution at 60 DEG C, drying and crushing, so as to obtain a brown powder extract; adding deionized water equal to 100 times of the weight of the brown powder extract to prepare a solution, enabling the solution to flow through a preprocessed macroporous absorptive resin layer, eluting the column layer at constant speed until the eluate is colourless, finally eluting with an eluent at the same speed, collecting the eluate, and concentrating and drying to obtain red powder, namely the symbiotic pigment of the red sorghum starch. The method is simple in process, strong in operability, applicable to industrial production and good in separation effect; the extraction-separation rate of the symbiotic pigment of the red sorghum starch is 93.4%, the yield of a symbiotic pigment product of the red sorghum starch is 0.13%, the purify of the symbiotic pigment product of the red sorghum starch is 92.1%, and the symbiotic pigment product is an isoflavone substance.
Description
Technical field
The present invention relates to a kind of extraction and separation method of sorghum starch symbiosis pigment, belong to food purifying and preparation method thereof field.
Background technology
Starch is the important natural ergastic substances of nature and organic energy supply material, is composition important in cereal.Due to cereal starch and albumen, grease, the component symbiosis such as Mierocrystalline cellulose, to the research of cereal starch, first need to obtain the purifying starch sample of symbol containing purity requirement.
Chinese sorghum is grass, and its seed is the crop kernel of high-content of starch in cereal, on average starch-containing must 67%, sorghum seed is different in appearance from other cereal kernel, and sorghum seed often has natural color.Color classification in " the national sorghum variety resource exploration Recording criteria " that pass through with the Ministry of Agriculture, in 3001 parts of Chinese sorghum samples that the whole nation collects, yellow seed 817 kinds, brown 734 kinds, red 1058 kinds, purple 5 kinds, white 387 kinds, in sorghum seed, red seed ratio is maximum, when purifying sorghum starch, needs these natural pigment extraction and isolation.
Therefore, study a kind of technique simple, workable, the extraction and separation method being applicable to suitability for industrialized production and good separating effect has certain economic and social profit.
Summary of the invention
It is simple, workable that the present invention aims to provide a kind of technique, is applicable to the extraction and separation method of the red sorghum starch symbiosis pigment of suitability for industrialized production and good separating effect.
The extraction and separation method of described red sorghum starch symbiosis pigment, comprises the following steps:
The Chinese sorghum seed of redness is cleaned, dry, pulverized 60 mesh sieves, the powder after sieving is added in extracting solution and extracts, filter, extracting solution is concentrated under 60 DEG C of conditions, dry, pulverize to obtain brown ceramic powder extract; Deionized water extract being added 100 times of weight becomes wiring solution-forming, with certain flow velocity through pretreated macroporous adsorptive resins layer, post layer is washed with same flow velocity with deionized water, to elutriant is colourless, finally use elutriant with same flow velocity wash-out, collect elutriant, concentrated, drying obtains red powder and red sorghum starch symbiosis pigment.
Preferably, extraction conditions of the present invention is extracting solution is 80% aqueous ethanolic solution, and Extracting temperature is 35-45 DEG C, and extraction time is 1h, and material-water ratio is 1:15.
Preferred, elutriant of the present invention is 30% aqueous ethanolic solution, and elution flow rate is 25mL/cm
2h.
Preferred further, macroporous adsorbent resin of the present invention is HPD-600 macroporous adsorbent resin or AB-8 macroporous adsorbent resin.
Further preferred, the pre-treatment of macroporous adsorbent resin of the present invention is that resin is put into container, after adding ethanol infiltration, resin is filled into resin column, the alcohol immersion 4h higher than resin layer 10cm is added in post, release immersion liquid, with ethanol be washed till washings thin up in test tube not muddy till, then be less than 1% with deionized-distilled water repetitive scrubbing to ethanol content.
The extraction and separation method of red sorghum starch symbiosis pigment of the present invention, has technique simple, workable, is applicable to the feature such as suitability for industrialized production and good separating effect.After measured, the extraction and isolation rate of red sorghum starch symbiosis pigment is 93.4% to the red sorghum starch symbiosis pigment obtained with extraction and isolation of the present invention, and red sorghum starch symbiosis pigment product yield is 0.13%, and purity is 92.1%, is isoflavone like substance.
Embodiment
Embodiment one:
The Chinese sorghum seed 1000g of redness is cleaned, dry, pulverized 60 mesh sieves, 80% aqueous ethanolic solution that the powder after sieving adds 15L is extracted 1h at 40 DEG C, filters, extracting solution is concentrated under 60 DEG C of conditions, dry, pulverize to obtain brown ceramic powder extract 2.7g, extract is added 270mL deionized water and become wiring solution-forming, with 25mL/cm
2the flow velocity of h is through (the pre-treatment: resin is put into container of pretreated HPD-600 macroporous adsorptive resins layer, after adding ethanol infiltration, resin is filled into resin column, the alcohol immersion 4h higher than resin layer 10cm is added in post, release immersion liquid, with ethanol be washed till washings thin up in test tube not muddy till, 1% is less than to ethanol content again) with deionized-distilled water repetitive scrubbing, post layer is washed with same flow velocity with deionized water, to elutriant is colourless, last with the same flow velocity wash-out of 30% ethanol, collect elutriant, concentrated, drying obtains red powder and red sorghum starch symbiosis pigment 1.3g.As calculated, extraction and separation method of the present invention, the extraction and isolation rate of red sorghum starch symbiosis pigment is 93.4%, and red sorghum starch symbiosis pigment product yield is 0.13%, and purity is 92.1%, is isoflavone like substance.
Embodiment two:
The Chinese sorghum seed 1000g of redness is cleaned, dry, pulverized 60 mesh sieves, 80% aqueous ethanolic solution that powder after sieving adds 15L is extracted 1h at 40 DEG C, filter, extracting solution is concentrated under 60 DEG C of conditions, dry, pulverize to obtain brown ceramic powder extract 2.62g, extract is added 260mL deionized water and become wiring solution-forming, with 25mL/cm
2the flow velocity of h is through (the pre-treatment: resin is put into container of pretreated AB-8 macroporous adsorptive resins layer, after adding ethanol infiltration, resin is filled into resin column, the alcohol immersion 4h higher than resin layer 10cm is added in post, release immersion liquid, with ethanol be washed till washings thin up in test tube not muddy till, 1% is less than to ethanol content again) with deionized-distilled water repetitive scrubbing, post layer is washed with same flow velocity with deionized water, to elutriant is colourless, last with the same flow velocity wash-out of 30% ethanol, collect elutriant, concentrated, drying obtains red powder and red sorghum starch symbiosis pigment 1.21g.As calculated, extraction and separation method of the present invention, the extraction and isolation rate of red sorghum starch symbiosis pigment is 92.3%, and red sorghum starch symbiosis pigment product yield is 0.121%, and purity is 91.6%, is isoflavone like substance.
Claims (5)
1. the extraction and separation method of red sorghum starch symbiosis pigment, comprises the following steps:
The Chinese sorghum seed of redness is cleaned, dry, pulverized 60 mesh sieves, the powder after sieving is added in extracting solution and extracts, filter, extracting solution is concentrated under 60 DEG C of conditions, dry, pulverize to obtain brown ceramic powder extract; Deionized water extract being added 100 times of weight becomes wiring solution-forming, with certain flow velocity through pretreated macroporous adsorptive resins layer, post layer is washed with same flow velocity with deionized water, to elutriant is colourless, finally use elutriant with same flow velocity wash-out, collect elutriant, concentrated, drying obtains red powder and red sorghum starch symbiosis pigment.
2. the extraction and separation method of red sorghum starch symbiosis pigment as claimed in claim 1, it is characterized in that described extraction conditions be extracting solution is 80% aqueous ethanolic solution, Extracting temperature is 35-45 DEG C, and extraction time is 1h, and material-water ratio is 1:15.
3. the extraction and separation method of red sorghum starch symbiosis pigment as claimed in claim 1, it is characterized in that described elutriant is 30% aqueous ethanolic solution, elution flow rate is 25mL/cm
2h.
4. the extraction and separation method of red sorghum starch symbiosis pigment as claimed in claim 1, is characterized in that described macroporous adsorbent resin is HPD-600 macroporous adsorbent resin or AB-8 macroporous adsorbent resin.
5. the extraction and separation method of the red sorghum starch symbiosis pigment as described in claim 1 or 4, it is characterized in that the pre-treatment of described macroporous adsorbent resin is that resin is put into container, after adding ethanol infiltration, resin is filled into resin column, the alcohol immersion 4h higher than resin layer 10cm is added in post, release immersion liquid, with ethanol be washed till washings thin up in test tube not muddy till, then be less than 1% with deionized-distilled water repetitive scrubbing to ethanol content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410569824.0A CN104371350A (en) | 2014-10-23 | 2014-10-23 | Method for extracting and separating symbiotic pigment of red sorghum starch |
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| CN201410569824.0A CN104371350A (en) | 2014-10-23 | 2014-10-23 | Method for extracting and separating symbiotic pigment of red sorghum starch |
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| CN104371350A true CN104371350A (en) | 2015-02-25 |
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| CN201410569824.0A Pending CN104371350A (en) | 2014-10-23 | 2014-10-23 | Method for extracting and separating symbiotic pigment of red sorghum starch |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305750A (en) * | 2019-07-03 | 2019-10-08 | 富民县梓源贸易有限责任公司 | A kind of red sorghum wine and its brewing method |
Citations (5)
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|---|---|---|---|---|
| CN86105236A (en) * | 1986-08-08 | 1987-02-11 | 邱文民 | A kind of extracting method of natural red colouring matter |
| CN1064284A (en) * | 1991-08-13 | 1992-09-09 | 青岛化工研究所 | Method for extracting sorghum red pigment |
| CN1065079A (en) * | 1991-03-15 | 1992-10-07 | 陕西省粮油科学研究所 | The method of extracting Chinese sorghum red pigment by use of water |
| CN101284022A (en) * | 2007-06-01 | 2008-10-15 | 山西省农业科学院农产品综合利用研究所 | Complexation extraction method of plant polyphenols substances |
| CN102675905A (en) * | 2011-03-16 | 2012-09-19 | 杨青松 | Method for extracting sorghum red pigment |
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2014
- 2014-10-23 CN CN201410569824.0A patent/CN104371350A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86105236A (en) * | 1986-08-08 | 1987-02-11 | 邱文民 | A kind of extracting method of natural red colouring matter |
| CN1065079A (en) * | 1991-03-15 | 1992-10-07 | 陕西省粮油科学研究所 | The method of extracting Chinese sorghum red pigment by use of water |
| CN1064284A (en) * | 1991-08-13 | 1992-09-09 | 青岛化工研究所 | Method for extracting sorghum red pigment |
| CN101284022A (en) * | 2007-06-01 | 2008-10-15 | 山西省农业科学院农产品综合利用研究所 | Complexation extraction method of plant polyphenols substances |
| CN102675905A (en) * | 2011-03-16 | 2012-09-19 | 杨青松 | Method for extracting sorghum red pigment |
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| Title |
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| 刘钟栋等: "高粱子粒淀粉表面色素的树脂分离研究", 《离子交换与吸附》 * |
| 刘钟栋等: "高粱籽粒淀粉表面色素的树脂分离研究", 《中国食品添加剂》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305750A (en) * | 2019-07-03 | 2019-10-08 | 富民县梓源贸易有限责任公司 | A kind of red sorghum wine and its brewing method |
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