CN104352529B - The preparation of blattaria anti-hepatic fibrosis activity extract and detection method - Google Patents
The preparation of blattaria anti-hepatic fibrosis activity extract and detection method Download PDFInfo
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Abstract
The present invention relates to the preparation method and detection method of a kind of cockroach extractive, preparation method includes：Blattaria adult is killed into crushed after being dried；Gained powder addition alcoholic solvent is extracted and filtered；Gained filtrate decompression is concentrated into medicinal extract without alcohol taste；Medicinal extract and hot water obtained by being added in oily-water seperating equipment, are stirred, and water layer is released after standing, collect filtrate and filter；Blattaria degreasing extract is adsorbed, then eluted with the alcohol of water and various concentration by large pore resin absorption column；By the filtering of gained eluent, reduced pressure, crushed after being dried to get the activity extract of anti-hepatic fibrosis；The content of total peptide in extract active constituent, mucopolysaccharide, polyalcohol and free amino acid is measured respectively, it is chiefly used in scalding currently for cockroach extractive, burns, organizing to repair, the application in terms of oral care and angiocardiopathy, the present invention makes the medical value of blattaria further be promoted.
Description
Technical field
The present invention relates to pharmaceutical technology field, specifically, the present invention relates to a kind of extractions of blattaria anti-hepatic fibrosis activity
The preparation method of object and the detection method of active component.
Background technology
Blattaria (Blattodea) belongs to Insecta, Blattaria, and Blattidae is commonly called as cockroach.It has survived 4 on earth
100000000 years, be in the world vitality it is most strong, it is most ancient, multiply one of most successful insect assembly so far.Blattaria is regarded always by the mankind
For pest, but it in face of the mankind various killing means can indomitable procreation so far, undoubtedly there is classic biology in vivo
Active material and special physiological mechanism.
In recent years, with the attention that China develops Chinese Traditional Medicine the Study on Resources, scientific worker is rich to this resource
Rich insect has carried out the research and development of application aspect, achieves gratifying achievements, and using blattaria medicinal material as raw material, successively research and develop
New drug have：" Kangfuxin Liquid ", " the grand capsule of liver ", " Xinmailong injection ", wherein " the grand capsule of liver " and " Xinmailong injection "
For national two kind new medicines (Chinese medicine)." Kangfuxin Liquid " is for treating canker sore, duodenal ulcer, burn, scald, cotton-padded mattress
Sore, pulmonary tuberculosis auxiliary treatment etc. protect kind, national medical insurance Class B drug for national Chinese medicine；" the grand capsule of liver " is slow for treating
Property hepatitis B, the liver diseases such as hepatic sclerosis and HBV carrier；" Xinmailong injection " cures mainly chronic congestion heart failure
It exhausts, chronic pulmonary heart disease, it is particularly evident to secondary pulmonary heart disease, the heart failure therapeutic effect of cardiomyopathy.The researchs such as Hao Fangfang
American cockroach (periplaneta americana are one kind of blattaria) ethanol extract is to fibrosis rat
Influence [the document of liver function：Hao Fangfang, Li Chunyan, Lai Yong wait American cockroaches to fibrosis Liver Function
Influence [J] Agriculture of Anhui science, 2012,40 (18):9717.], the results showed that American cockroach ethanol extract is to immunological liver
Fibrosis has certain preventive and therapeutic effect, can be obviously improved liver function；The result of study of sweet equality shows that American cockroach ethyl alcohol carries
Hepatic injury [document caused by object has the function of anti-CCl4 and ConA：Gan Ping, Zhang Xuqiang, He Xu wait American cockroach extracts to small
Protective effect [J] the modern medicines of mouse acute liver damage are with clinical, 2011,26 (2):123-128.].
In order to have the active site of effect of anti hepatic fibrosis in clear and definite blattaria medicinal material, further understand fully and wherein play main work
Material base, and the mechanism of action of blattaria anti-hepatic fibrosis is illustrated on the basis of molecular pharmacology, it is unfolded in inventor
Detailed pharmaceutical procedures research.Inventor uses the alcohol extracting of various concentration, and extracting method uses the common cold soaking of industrial production
Or heat puies forward two methods, subtractive process select macroreticular resin that price is relatively low, is easy to repeated regeneration utilizes to cockroach extractive into
Row research, and the inside and outside experiment of combination carries out tracking activity, obtains with the stronger active site of effect of anti hepatic fibrosis.
The present invention selects the ethyl alcohol of various concentration to extract blattaria first, obtains crude extract, and use rats'liver star
Shape cell strain (HSC-T6) carries out screening active ingredients to crude extract, filters out the most strong crude extract of activity, then selects a variety of
Macroreticular resin is separation material, and active crude extract is isolated and purified, while establishes liver using healthy cleaning grade SD rats
Fibrosis model carries out anti-hepatic fibrosis pharmacological activity experiment to the cockroach extractive isolated and purified, passes through mathematical statistics
Analysis, screening obtain the most strong activity extract of anti-hepatic fibrosis activity.
Advantage of the invention is that：1. blattaria is resourceful, artificial feeding has been fully achieved at present, and national annual output reaches
More than 600 tons or more；2. the obtained cockroach extractive of the present invention has safely, effectively, the quality controllable, advantage that has no toxic side effect；
The material that 3. extracting and developing, purified material used in present invention process are environmental protection, safety, iterative regenerable utilize, as alcohol,
Macroreticular resin；One of five big serial " cloud medicine " distinguishing products that 4. research and development of blattaria medicine series, which are Yunnan Province's emphasis, cultivates, should
The natural products that achievement in research obtains, by for research and develop safely, effectively, it is resourceful, there is stronger effect of anti hepatic fibrosis
New drug lays the foundation, and the pharmaceutical insects for making this resourceful are preferably serviced for the mankind.
Invention content
It is a primary object of the present invention to provide a kind of ethyl alcohol extraction, purification with macroreticular resin, it is fine to prepare the anti-liver of blattaria
The preparation of the extract of dimensionization effect and detection method.
The preparation method of effective part extract of the blattaria with anti-hepatic fibrosis is as follows in the present invention：
Feedstock processing：Adult of the blattaria culturing time at 5 months or more is scalded after death with 50 DEG C~60 DEG C of hot water, through 60
Crushed after being dried at a temperature of~70 DEG C.
Extraction：Gained powder is placed in extractor, alcoholic solvent is added in and extracts obtained filtrate；
Wherein alcoholic solvent be methanol, the mixture of ethyl alcohol, one or more of ethylene glycol with water, extract 2~3 times, often
The secondary solvent with 3~7 times of amounts, solvent strength are 60%~95%, and extracting method uses cold soaking extraction method or reflux extraction.It is cold
Extraction is followed the example of as blattaria drying adult is crushed to 10~30 mesh, is put in steeping tank, add 3~7 times of amount volume fractions for 60~
95% Extraction solvent, stirs evenly, and impregnates every time 72 hours or more, primary at interval of stirring in 3~6 hours therebetween, then will extraction
Liquid aperture is the filter-cloth filtering of 200~500 mesh, collects filtrate；The reflux extraction is to be crushed to blattaria drying adult
10~30 mesh, put in extractor, add the Extraction solvent that 3~7 times of amount volume fractions are 60~95%, stir evenly, at 70~80 DEG C
Refluxing extraction 2~5 hours is extracted 3 times, then by the filter-cloth filtering that extracting solution aperture is 200~500 mesh, collects filtrate；Institute
State the one kind that filters and can first use in cotton filter cloth, cotton synthetic fibre filter cloth, terylene filter cloth, polypropylene fibre filter cloth or nylon filtering cloth.
Concentration：Gained filtrate decompression is concentrated into medicinal extract without alcohol taste to get cockroach extractive；
Degreasing：Medicinal extract and hot water obtained by being added in oily-water seperating equipment, are stirred, water layer are released after standing, collect
Filtrate is simultaneously filtered to get blattaria degreasing extract；
The hot water for 2~5 times of 60~75 DEG C of amounts that medicinal extract and medicinal extract volume are added in oily-water seperating equipment are wherein used,
It is slowly stirred along same direction, and is heated to 65~75 DEG C, keep the temperature 0.5 hour, stand cooling 6~12 hours, treat grease point
From rear releasing water layer, filtrate is collected in filtering
Absorption, elution：Blattaria degreasing extract is adsorbed, then by large pore resin absorption column with water and the alcohol of various concentration
60~95% eluent is washed in elution, collection；
Macroporous absorbent resin is graininess, and the alcoholic solution of various concentration refers to 50~95% ethyl alcohol, macroporous absorbent resin
Dosage be 1~5 times of degreasing extracting solution amount volume, degreaser upper prop is washed after adsorbing 6~12 hours with 50~95% ethyl alcohol
It is de-, collect eluent, filtering.
Manufactured goods：By the filtering of gained eluent, reduced pressure, crushed after being dried to get the active constituent of extract；Decompression
Temperature must not exceed 75 DEG C during concentration.
It measures：1. the measure of total peptide in extract：Using Folin- phenol methods, this product is containing polypeptide with balf serum albumin
Meter, must not be less than 400.0mg/g.2. the measure of mucopolysaccharide in extract：Using phend-sulphuric acid, this product is containing polypeptide with grape
Sugar meter, must not be less than 100.0mg/g.3. the measure of polyalcohol in extract：Using potassium metaperiodate-Nash methods, this product is containing polynary
Alcohol must not be less than 70.0mg/g in terms of mannitol.4. the measure of free amino acid in extract：Using ninhydrin method, this product contains
Free amino acid must not be less than 200.0mg/g in terms of tyrosine.
The beneficial effects of the present invention are：There is provided it is a kind of extracted with ethyl alcohol, purification with macroreticular resin, prepare the anti-liver of blattaria
The preparation of the extract of Fibrosis parameters and detection method solve and are chiefly used in oral care and the heart currently for cockroach extractive
Application in terms of vascular diseases makes the medical value of blattaria further be promoted.
Specific embodiment
The embodiment of the present invention is discussed in detail below.It should be noted that the technical characteristic described in following embodiments
Or the combination of technical characteristic is not construed as isolated, they can be combined with each other and be combined with each other to reach more
Good technique effect.Specific extract preparation process is as follows：
Feedstock processing：Adult of the blattaria culturing time at 5 months or more is scalded after death with 50 DEG C~60 DEG C of hot water, through 60
Crushed after being dried at a temperature of~70 DEG C.
Extraction：Gained powder is placed in extractor, alcoholic solvent is added in and extracts obtained filtrate；
Wherein alcoholic solvent be methanol, the mixture of ethyl alcohol, one or more of ethylene glycol with water, extract 2~3 times, often
The secondary solvent with 3~7 times of amounts, solvent strength are 60%~95%, and extracting method uses cold soaking extraction method or reflux extraction.It is cold
Extraction is followed the example of as blattaria drying adult is crushed to 10~30 mesh, is put in steeping tank, add 3~7 times of amount volume fractions for 60~
95% Extraction solvent, stirs evenly, and impregnates every time 72 hours or more, primary at interval of stirring in 3~6 hours therebetween, then will extraction
Liquid aperture is the filter-cloth filtering of 200~500 mesh, collects filtrate；The reflux extraction is to be crushed to blattaria drying adult
10~30 mesh, put in extractor, add the Extraction solvent that 3~7 times of amount volume fractions are 60~95%, stir evenly, at 70~80 DEG C
Refluxing extraction 2~5 hours is extracted 3 times, then by the filter-cloth filtering that extracting solution aperture is 200~500 mesh, collects filtrate；Institute
State the one kind that filters and can first use in cotton filter cloth, cotton synthetic fibre filter cloth, terylene filter cloth, polypropylene fibre filter cloth or nylon filtering cloth.
Concentration：Gained filtrate decompression is concentrated into medicinal extract without alcohol taste to get cockroach extractive；
Degreasing：Medicinal extract and hot water obtained by being added in oily-water seperating equipment, are stirred, water layer are released after standing, collect
Filtrate is simultaneously filtered to get blattaria degreasing extract；
The hot water for 2~5 times of 60~75 DEG C of amounts that medicinal extract and medicinal extract volume are added in oily-water seperating equipment are wherein used,
It is slowly stirred along same direction, and is heated to 65~75 DEG C, keep the temperature 0.5 hour, stand cooling 6~12 hours, treat grease point
From rear releasing water layer, filtrate is collected in filtering
Absorption, elution：Blattaria degreasing extract is adsorbed, then by large pore resin absorption column with water and the alcohol of various concentration
60~95% eluent is washed in elution, collection；
Macroporous absorbent resin is graininess, and the alcoholic solution of various concentration refers to 50~95% ethyl alcohol, macroporous absorbent resin
Dosage be 1~5 times of degreasing extracting solution amount volume, degreaser upper prop is washed after adsorbing 6~12 hours with 50~95% ethyl alcohol
It is de-, collect eluent, filtering.
Manufactured goods：By the filtering of gained eluent, reduced pressure, crushed after being dried to get the active constituent of extract；Decompression
Temperature must not exceed 75 DEG C during concentration.
It measures：1. the measure of total peptide in extract：Using Folin- phenol methods, this product is containing polypeptide with balf serum albumin
Meter, must not be less than 400.0mg/g.2. the measure of mucopolysaccharide in extract：Using phend-sulphuric acid, this product is containing polypeptide with grape
Sugar meter, must not be less than 100.0mg/g.3. the measure of polyalcohol in extract：Using potassium metaperiodate-Nash methods, this product is containing polynary
Alcohol must not be less than 70.0mg/g in terms of mannitol.4. the measure of free amino acid in extract：Using ninhydrin method, this product contains
Free amino acid must not be less than 200.0mg/g in terms of tyrosine.
Embodiment 1
The preparation of anti-hepatic fibrosis activity extract of the blattaria alcohol extracting thing after AB-8 purification with macroreticular resin and master
Want the measure of active constituent
1st, by blattaria culturing time in the drying medicinal material of 5 months or more through 60~70 DEG C of crushed after being dried, put in extractor,
Add the alcohol solvent of 3~5 times of amounts a concentration of 60~90%, refluxing extraction or cold soaking extraction can be used.Refluxing extraction：Extraction three
Secondary, 3~5 hours for the first time, second and third time 2~4 hours, reflux temperature was controlled at 75~80 DEG C, was 200~500 mesh with aperture
Filter-cloth filtering, merging filtrate.Cold soaking extracts：Extraction three times, is impregnated 72 hours or more, therebetween at interval of 6~12 hours every time
Stirring is primary, with the filter-cloth filtering that aperture is 200~500 mesh, merging filtrate.
2nd, above-mentioned filtrate decompression is concentrated, temperature is 50~70 DEG C, is concentrated into medicinal extract without alcohol taste, and relative density is at this time
1.05~1.20 to get blattaria alcohol extract.
3rd, the hot water of 3 times of 60~75 DEG C of amounts of liquid extract and medicinal extract volume is added in oily-water seperating equipment, along one
A direction is slowly stirred, and mixing speed is 30~50 revs/min, while is heated to 65~75 DEG C, keeps the temperature 1 hour, stands cooling
12 hours, water layer is released after grease separation, with 200~500 mesh filter-cloth filterings, collects filtrate, filtrate decompression concentration, temperature is
55~65 DEG C, relative density is concentrated into as 1.1~1.3 to get blattaria degreasing extract.
4th, the segmentation of AB-8 macroporous absorbent resins is refined：By the ethanol extract of blattaria 50~95%, upper macropore is inhaled after degreasing
Attached resin column, and adsorbing 2~3 hours, is washed with water colourless to eluent, with the ethanol elution of various concentration, collects 60~95%
Ethanol elution, filtering, filtrate decompression concentration, temperature be 60 DEG C, freeze-drying or spray drying, crush to get the present invention
Activity extract.
Macroporous absorbent resin is graininess, and the dosage of macroporous absorbent resin is 3~5 times of amount volumes of degreasing extracting solution.
5th, it measures
5.1 measure the total peptide content in cockroach extractive using Folin- phenol method, and this product is containing polypeptide with the white egg of calf serum
White meter, must not be less than 400.0mg/g.
5.1.1 balf serum albumin reference substance 0.2g, it is accurately weighed, it puts in 100ml measuring bottles, adds water to scale, shake up.
Precision measures 10ml, is placed in 100ml measuring bottles, adds water to scale, shakes up to get (per 1ml 200 μ containing balf serum albumin
g)。
5.1.2 accurate reference substance solution 0.00,0.10,0.20,0.40,0.60,0.80, the 1.00m of measuring is in 10ml measuring bottles
In, add distilled water polishing to 1.00ml.It often manages and respectively adds alkaline copper reagent (NaOH containing 0.5mol/l, 10%Na2CO3,0.1%
NaKC4H4O6 and 0.05%CuSO4) 1.0ml, mixing.35 DEG C of heating reaction 10min, cooling；Rapidly join Folin- successively again
Phenol reagent 4.0ml, mixing, 55 DEG C of heating reaction 15min, cooling adds water constant volume, with 0.00 a concentration of blank of reference substance solution,
Absorbance is measured at 760nm wavelength according to UV-VIS spectrophotometry, using absorbance as ordinate, a concentration of abscissa is painted
Standard curve processed.
5.1.3 this product 1.0g is taken, it is accurately weighed, add appropriate distilled water ultrasonic dissolution, be settled to 100ml with water, examination is provided
Product solution.Precision pipettes test solution 1.0ml, and prepared by the method under the preparation of sighting target directrix curve, measure absorbance in accordance with the law,
And the content of total peptide in test solution is calculated with standard curve.
5.2 use phend-sulphuric acid, and this product, with glucose meter, must not be less than 100.0mg/g containing polypeptide.
5.2.1 it is accurate to weigh the dry glucose control product 100mg to constant weight, it puts in 100ml volumetric flasks, is dissolved in water,
Constant volume shakes up reference substance storing solution to get 1.0mg/ml.It is appropriate that precision pipettes storing solution, is diluted with distilled water into 0.1mg/
The reference substance test fluid of ml.
5.2.2 precision weighs cockroach extractive ML-D freeze-dried powders about 50mg in 100ml round-bottomed flasks, adds 6mol/L hydrochloric acid
50ml, sealing, puts in 90 DEG C of thermostatic drying chambers and hydrolyzes 1h, cools down, is transferred in 100ml volumetric flasks, quarter is settled to distilled water
Degree, shakes up to get test solution.
5.2.3 the accurate test solution 10ml, enriching sulfuric acid 20ml, 5% phenol 2ml, in boiling water bath pipetted after hydrolysis
Middle heating colour developing 10min measures absorbance at 490nm, calculates the content of mucopolysaccharide in test sample.
5.3 use potassium metaperiodate-Nash methods, and this product in terms of mannitol, must not be less than 70.0mg/g containing polyalcohol.
5.3.1 it is accurate to weigh the dry mannitol reference substance 10mg to constant weight, it puts in 10ml measuring bottles, is dissolved in water, constant volume,
Shake up the reference substance storing solution to get 1.0mg/ml.Precision pipettes reference substance storing solution 4ml in 100ml measuring bottles, uses distilled water
Constant volume is shaken up to get the reference substance test fluid of 40 μ g/ml.
5.3.2 precision weighs cockroach extractive ML-D freeze-dried powders about 30mg in 100ml measuring bottles, with distilled water constant volume, shakes
The even test liquid to get 0.3mg/ml.
5.3.3 test solution and each 5ml of distilled water are separately added into 1ml0.015mol/L periodic acid in 25ml measuring bottles
Potassium solution is stored at room temperature 15min, is separately added into 2ml0.1%L- rhamnoses, adds 4mlNash test solutions, 50 DEG C of heat preservations
15min, cooling, is settled to scale with distilled water, shakes up.With absorbance is measured at ultraviolet-visible spectrophotometer 413nm, count
Calculate the content of polyalcohol in test sample.
5.4 use ninhydrin method, and this product in terms of tyrosine, must not be less than 200.0mg/g containing free amino acid.
5.4.1 the accurate tyrosine reference substance 10.0mg for weighing drying to constant weight is put in 100ml volumetric flasks, adds 0.02mol/
The sodium hydroxide solution 10ml of L, heating water bath are settled to scale to being completely dissolved, with distilled water, and mixing is to get a concentration of
The reference substance solution of 0.1mg/ml.
5.4.2 precision weighs ML-D 25mg and puts in 100ml measuring bottles, with distillation water dissolution and is settled to scale, mixing, i.e.,
Obtain the test solution of a concentration of 0.25mg/ml.
5.4.3 precision pipettes 1ml test solutions and 1ml distilled water is put respectively in 10ml measuring bottles, adds 1ml1.0% respectively
Ninhydrin solution and 1ml citrate buffer solutions (pH=6.0), mixing, boiling water bath heating colour developing 30min, cooling, respectively plus
3ml60% ethyl alcohol is settled to scale with distilled water, and mixing measures absorbance value at 570nm, calculates containing for free amino acid
Amount.
Embodiment 2
The preparation of anti-hepatic fibrosis activity extract of the blattaria alcohol extracting thing after D-101 purification with macroreticular resin and master
Want the measure of active constituent
The preparation of 1 blattaria activity extract：Extraction, concentration, degreasing process are the same as the 1~3 of embodiment one.
The segmentation of 2D-101 macroporous absorbent resins is refined：Object is taken to go up D- after adding appropriate water dissolution blattaria obtained in the previous step
101 large pore resin absorption columns, absorption overnight, be washed with water it is colourless to eluent, then with the alcohol of various concentration elute, collect 60~
95% ethanol eluate, filtering, filtrate decompression concentration, temperature are 60 DEG C, and freeze-drying or spray drying are crushed to get this
The activity extract of invention.
Macroporous absorbent resin is graininess, and the dosage of macroporous absorbent resin is 3 times of amount volumes of degreasing extracting solution.
3 measure：1. the measure of total peptide in extract：Using Folin- phenol methods, this product is containing polypeptide with balf serum albumin
Meter, measured value 461.0mg/g.2. the measure of mucopolysaccharide in extract：Using phend-sulphuric acid, this product is containing polypeptide with grape
Sugar meter, measured value 137.0mg/g.3. the measure of polyalcohol in extract：Using potassium metaperiodate-Nash methods, this product is containing polynary
Alcohol is in terms of mannitol, measured value 78.5mg/g.4. the measure of free amino acid in extract：Using ninhydrin method, this product contains
Free amino acid is in terms of tyrosine, measured value 237.5mg/g.
Embodiment 3
The preparation of anti-hepatic fibrosis activity extract of the blattaria alcohol extracting thing after D-201 purification with macroreticular resin and master
Want the measure of active constituent
The preparation of 1 blattaria activity extract：Extraction, concentration, degreasing process are the same as the 1~3 of embodiment one.
The segmentation of 2D-201 macroporous absorbent resins is refined：Object is taken to go up D- after adding appropriate water dissolution blattaria obtained in the previous step
201 large pore resin absorption columns, absorption overnight, be washed with water it is colourless to eluent, then with the alcohol of various concentration elute, collect 60~
95% ethanol eluate, filtering, filtrate decompression concentration, temperature are 60 DEG C, and freeze-drying or spray drying are crushed to get this
The activity extract of invention.
Macroporous absorbent resin is graininess, and the dosage of macroporous absorbent resin is 3 times of amount volumes of degreasing extracting solution.
3 measure：1. the measure of total peptide in extract：Using Folin- phenol methods, this product is containing polypeptide with balf serum albumin
Meter, measured value 462.1mg/g.2. the measure of mucopolysaccharide in extract：Using phend-sulphuric acid, this product is containing polypeptide with grape
Sugar meter, measured value 127.2mg/g.3. the measure of polyalcohol in extract：Using potassium metaperiodate-Nash methods, this product is containing polynary
Alcohol is in terms of mannitol, measured value 88.6mg/g.4. the measure of free amino acid in extract：Using ninhydrin method, this product contains
Free amino acid is in terms of tyrosine, measured value 231.6mg/g.
Embodiment 4
The preparation of anti-hepatic fibrosis activity extract of the blattaria alcohol extracting thing after HPD-100 purification with macroreticular resin and
The measure of main active
The preparation of 1 blattaria activity extract：Extraction, concentration, degreasing process are the same as the 1~3 of embodiment one.
The segmentation of 2HPD-100 macroporous absorbent resins is refined：Object is taken to go up D- after adding appropriate water dissolution blattaria obtained in the previous step
201 large pore resin absorption columns, absorption overnight, be washed with water it is colourless to eluent, then with the alcohol of various concentration elute, collect 60~
95% ethanol eluate, filtering, filtrate decompression concentration, temperature are 60 DEG C, and freeze-drying or spray drying are crushed to get this
The activity extract of invention.
Macroporous absorbent resin is graininess, and the dosage of macroporous absorbent resin is 3~5 times of amount volumes of degreasing extracting solution.
3 measure：1. the measure of total peptide in extract：Using Folin- phenol methods, this product is containing polypeptide with balf serum albumin
Meter, measured value 450.3mg/g.2. the measure of mucopolysaccharide in extract：Using phend-sulphuric acid, this product is containing polypeptide with grape
Sugar meter, measured value 130.5mg/g.3. the measure of polyalcohol in extract：Using potassium metaperiodate-Nash methods, this product is containing polynary
Alcohol is in terms of mannitol, measured value 82.1mg/g.4. the measure of free amino acid in extract：Using ninhydrin method, this product contains
Free amino acid is in terms of tyrosine, measured value 223.8mg/g.
A kind of preparation of blattaria adult extract provided by the invention and detection method, can effectively extract can in blattaria body
For the active constituent of effect of anti hepatic fibrosis, pass through Folin- phenol method, phend-sulphuric acid, potassium metaperiodate-Nash methods and indenes three
Ketone method is detected active constituent.
Although having been presented for some embodiments of the present invention herein, it will be appreciated by those of skill in the art that
Without departing from the spirit of the invention, the embodiments herein can be changed.Above-described embodiment is only exemplary, no
It should be using the embodiments herein as the restriction of interest field of the present invention.
Claims (6)
1. a kind of preparation method of blattaria adult extract, it is characterised in that：Include the following steps：
Feedstock processing：Adult of the blattaria culturing time at 5 months or more is killed into crushed after being dried；
Extraction：Gained powder is placed in extractor, alcoholic solvent is added in and extracts obtained filtrate；
Concentration：Gained filtrate decompression is concentrated into medicinal extract without alcohol taste to get cockroach extractive；
Degreasing：Medicinal extract and hot water obtained by being added in oily-water seperating equipment, are stirred, and water layer is released after standing, collect filtrate
And it filters to get blattaria degreasing extract；
Absorption, elution：Blattaria degreasing extract is adsorbed, then washed with the alcohol of water and various concentration by large pore resin absorption column
It is de-, collect 60~95% eluent；
Manufactured goods：By the filtering of gained eluent, reduced pressure, crushed after being dried to get the active constituent of extract；
Detection：The content of total peptide in the active constituent of extract, mucopolysaccharide, polyalcohol and free amino acid is measured respectively；
Using cold soaking extraction method or reflux extraction, the cold soaking extraction method is that blattaria drying adult is crushed to 10 for the extraction
~30 mesh, put in steeping tank, add the Extraction solvent that 3~7 times of amount volume fractions are 60~95%, stir evenly, impregnate 72 hours every time
More than, it is primary at interval of stirring in 3~6 hours therebetween, then by the filter-cloth filtering that extracting solution aperture is 200~500 mesh, collect
Filtrate；The reflux extraction is that blattaria drying adult is crushed to 10~30 mesh, is put in extractor, adds 3~7 times of amount volumes point
Number is 60~95% Extraction solvent, is stirred evenly, refluxing extraction 2~5 hours at 70~80 DEG C, is extracted 3 times, then by extracting solution
With the filter-cloth filtering that aperture is 200~500 mesh, filtrate is collected；It is described filtering first with cotton filter cloth, cotton synthetic fibre filter cloth, terylene filter cloth,
One kind in polypropylene fibre filter cloth or nylon filtering cloth；
The degreasing uses the hot water for 2~5 times of 60~75 DEG C of amounts that medicinal extract and medicinal extract volume are added in oily-water seperating equipment,
It is slowly stirred along same direction, and is heated to 65~75 DEG C, keep the temperature 0.5 hour, stand cooling 6~12 hours, treat grease point
From rear releasing water layer, filtrate is collected in filtering.
2. the preparation method of blattaria adult extract according to claim 1, it is characterised in that：The feedstock processing is to use
50 DEG C~60 DEG C of hot water scalds after death, is dried at 60 DEG C~70 DEG C.
3. the preparation method of blattaria adult extract according to claim 1, it is characterised in that：Alcohol in the extraction step
Solvent is the mixture of one or more of methanol, ethyl alcohol, ethylene glycol with water, extracts 2~3 times, is measured every time with 3~7 times
Solvent, solvent strength are 60%~95%, and extracting method uses cold soaking extraction method or reflux extraction.
4. the preparation method of blattaria adult extract according to claim 1, it is characterised in that：The absorption, elution step
Rapid macroporous absorbent resin is graininess, and the alcoholic solution of various concentration refers to 50~95% ethyl alcohol, the dosage of macroporous absorbent resin
Volume is measured for 1~5 times of degreasing extracting solution, degreaser upper prop uses 60~95% ethanol elution, collection after adsorbing 6~12 hours
Eluent, filtering.
5. the preparation method of blattaria adult extract according to claim 1, it is characterised in that：The temperature during reduced pressure
Degree must not exceed 75 DEG C.
6. a kind of detection method of the obtained blattaria adult extract of preparation method as claimed in claim 1, it is characterised in that：Institute
It states detection method and refers to the total peptide content measured using Folin- phenol method in extract, measured in extract using phend-sulphuric acid
The content of mucopolysaccharide measures the content of polyalcohol in extract using potassium metaperiodate-Nash methods, is measured and extracted using ninhydrin method
The content of free amino acid in object.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214262A (en) * | 2008-01-09 | 2008-07-09 | 大理学院 | American cockroaches effective fraction for anti-tumor prepared by macroporous adsorption resin and use |
| CN101558874A (en) * | 2009-05-22 | 2009-10-21 | 杨卫祖 | American cockroach-honey refined flavor cream and preparation method thereof |
| CN102973608A (en) * | 2012-11-22 | 2013-03-20 | 大理学院 | Application of effective part of cockroach extract in preparing drug for inhibiting fungus growth |
-
2014
- 2014-11-07 CN CN201410623930.2A patent/CN104352529B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214262A (en) * | 2008-01-09 | 2008-07-09 | 大理学院 | American cockroaches effective fraction for anti-tumor prepared by macroporous adsorption resin and use |
| CN101558874A (en) * | 2009-05-22 | 2009-10-21 | 杨卫祖 | American cockroach-honey refined flavor cream and preparation method thereof |
| CN102973608A (en) * | 2012-11-22 | 2013-03-20 | 大理学院 | Application of effective part of cockroach extract in preparing drug for inhibiting fungus growth |
Non-Patent Citations (1)
| Title |
|---|
| 美洲大蠊提取物含药血清体外抗肝纤维化的实验研究;李春艳等;《中药材》;20130531;第36卷(第5期);第707-711页 * |
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