CN104345099B - A kind of measure the method for non-volatile organic acids in Nicotiana tabacum L. - Google Patents
A kind of measure the method for non-volatile organic acids in Nicotiana tabacum L. Download PDFInfo
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Abstract
A kind of measure the method for non-volatile organic acids in Nicotiana tabacum L.: first extract the treatment fluid that tobacco sample is corresponding: being pulverized by tobacco sample and be placed in food stage two-layer compound bag, every 1.0000g tobacco sample adds the adipic acid inner mark solution 1mL of methanolic solution 25 50mL and 2.0mg/mL；Then two-layer compound bag is placed on 600 900MPa pressure treatment 10 15min through hot-seal and obtains ultra high pressure treatment liquid；Then take ultra high pressure treatment liquid 10mL, add the 20 saturated NaCl solution of 30mL, extract 6 combining extraction liquids with dichloromethane, add 3.5g anhydrous sodium sulfate and be dried；Gas chromatogram is used to be measured the content of non-volatile organic acids in Nicotiana tabacum L. for confirmation extract afterwards.In the mensuration Nicotiana tabacum L. of the present invention, non-volatile organic acids is carried out at normal temperatures, and finding speed is fast, and side reaction does not occur, and measured value is close to theoretical value, reproducible, and reliability is high.
Description
Technical field:
The present invention relates to the extraction and determination applied technical field of tobacco component, specifically provide non-in a kind of mensuration Nicotiana tabacum L. waving
The method of the property sent out organic acid.
Background technology:
In prior art, non-volatile organic acids is one of main chemical compositions affecting Nicotiana tabacum L. organoleptic quality, particularly
Malic acid, oleic acid, linoleic acid and malonic acid, not only the content in Nicotiana tabacum L. is higher, and to the aroma quality of flue-cured tobacco and perfume quantity
Have a significant impact.Its scalable Nicotiana tabacum L. pH value, makes jealous improvement, and pH value of tobacco leaf have expressed the organic acid with inorganic component of Nicotiana tabacum L.
Soda balance state, it is possible to reflection flue gas intensity (nitrogenous bases) and zest, it is possible to indirectly reflect the armaticity of flue gas.Cigarette at present
In grass, the assay method of Non-volatile Organic Acids is of all kinds, and measurement result is the most different, respectively has its own pluses and minuses, visits
Seek and quickly measure the method for Non-volatile Organic Acids in Nicotiana tabacum L., can be to improve cigarette quality to provide data supporting, the most right
In Nicotiana tabacum L., the concrete assay method of non-volatile organic acids carries out studying and has important value.
Since the nineties in 20th century, supertension (Ultra High Pressure, UHP) technology refers to material is put into liquid
In medium, after acting on a period of time under 100～1000MPa pressure, there are many irreversible biochemical reactions in material, according to Le
Chatelier principle, the volume of material that high pressure can make reduces and accelerates response speed, utilizes supertension can realize the fast of organic acid
Speed, accurately analyze.At present, have no under ultra-high pressure condition, carry out the relevant report of Nicotiana tabacum L. organic acid mensuration.
Accordingly, it is desirable to obtain the method for non-volatile organic acids in the mensuration Nicotiana tabacum L. that a kind of technique effect is excellent.
Summary of the invention:
It is an object of the invention to provide the method for non-volatile organic acids in the mensuration Nicotiana tabacum L. that a kind of technique effect is excellent.
A kind of measuring the method for non-volatile organic acids in Nicotiana tabacum L., the non-volatile organic acids related generally to is the most following
One or a combination set of several: oxalic acid, malonic acid, succinic acid, lauric acid, positive ficocerylic acid, malic acid, positive 15 carbonic acid, Petiolus Trachycarpi
Acid, citric acid, stearic acid, oleic acid, linoleic acid, linolenic acid；Described Nicotiana tabacum L. specifically includes following one or a combination set of several: white
Rib Nicotiana tabacum L., cured tobacco leaf, atomatic tobacco, cabo, thin slice, expanded cut tobacco, blended type cigarette tobacco shred and Virginian-type cigarette tobacco shred；Its
It is characterised by: in described mensuration Nicotiana tabacum L., the method specific requirement of non-volatile organic acids is as follows:
First extract tobacco sample and obtain corresponding treatment fluid, extract process as follows: by tobacco sample (because there being ratio below
Example, so need not volume mass here) pulverize be placed on food stage two-layer compound bag (be not required to limit content here, logical
Often the most so write in paper) in, adding the sulfuric acid-methanol solution that concentration is 10-15%, every 1.0000g tobacco sample adds
Enter sulfuric acid-methanol solution 25-50mL；Every 1.0000g tobacco sample adds the adipic acid inner mark solution of concentration 2.0mg/mL
1mL；Then two-layer compound bag is placed on ultra high pressure treatment intracavity through hot-seal, through 600-900MPa pressure treatment so that complete
Becoming the esterification of non-volatile organic acids, the process time is the ultra high pressure treatment liquid that 10-15min obtains tobacco sample；
Then take ultra high pressure treatment liquid 10mL in 125mL separatory funnel, add the saturated NaCl solution of 20-30mL, every time
Extract with 10mL dichloromethane, extract 6 combining extraction liquids, add 3.5g anhydrous sodium sulfate and be dried overnight；
Use gas chromatogram to be measured extract afterwards, take the maximum recorded, in order to confirm in Nicotiana tabacum L. non-volatile
The content of property organic acid.
In described mensuration Nicotiana tabacum L., the preferred specific requirement of method of non-volatile organic acids is as follows:
First extract tobacco sample and obtain corresponding treatment fluid, extract process as follows: by tobacco sample (because there being ratio below
Example, so need not volume mass here) pulverize be placed on food stage two-layer compound bag (be not required to limit content here, logical
Often the most so write in paper) in, adding the sulfuric acid-methanol solution that concentration is 10%, every 1.0000g tobacco sample adds
Sulfuric acid-methanol solution 25-50mL；Every 1.0000g tobacco sample adds the adipic acid inner mark solution 1mL of concentration 2.0mg/mL；
Sample is 1:30 with the mass volume ratio of sulfuric acid-methanol solution；Then two-layer compound bag is placed on supertension through hot-seal
Processing intracavity, through 700MPa pressure treatment to complete the esterification of non-volatile organic acids, the process time is that 12min obtains cigarette
The ultra high pressure treatment liquid of grass sample；
Then take ultra high pressure treatment liquid 12mL in 125mL separatory funnel, add the saturated NaCl solution of 20-30mL, every time
Extract with 10mL dichloromethane, extract 6 combining extraction liquids, add 3.5g anhydrous sodium sulfate and be dried overnight；
Use gas chromatogram to be measured extract afterwards, take the maximum recorded, in order to confirm in Nicotiana tabacum L. non-volatile
The content of property organic acid.
The present invention gives the assay method of non-volatile organic acids in Nicotiana tabacum L., and experimental condition therein has been carried out deeply
The research entered, to farthest extracting the nonvolatile organic acids in tobacco sample, in Nicotiana tabacum L., non-volatile organic acids is true
Real-valued.The process technique of sample is mainly and considers following four factor: the concentration of sulfuric acid-methanol solution, sulfuric acid-methanol solution
Consumption, ultra high pressure, the process time.
The sulfuric acid-methanol solution concentration impact on non-volatile organic acids extracted amount: along with sulfuric acid-methanol solution concentration
Increasing, the extracted amount of non-volatile organic acids increases.When reaching 10%, the extracted amount of non-volatile organic acids reaches maximum, it
After declined along with the increase yield of concentration.Analyze its reason be likely due to concentration too low time, organic acid and the ester of methanol
Change reaction not exclusively, and during excessive concentration, easily cause the polymerization of some unsaturated acids.Sulfuric acid-methanol liquor capacity is to non-volatile
The impact of organic acid extracted amount: when being 1:25-40 from the mass volume ratio of experiment, cigarette sample and sulfuric acid-methanol solution, Nicotiana tabacum L.
The measured value of non-volatile organic acids is maximum.
The processing pressure impact on non-volatile organic acids measured value: processing pressure raises and is conducive to entering of esterification
OK.When processing pressure reaches 700MPa, the extracted amount of non-volatile organic acids reaches the highest, but too high processing pressure is anti-
And make the extracted amount of non-volatile organic acids reduce, it may be possible to occur poly-owing to unsaturated fatty acid can be caused during hypertonia
Close and affect esterification effect.
The process time impact on non-volatile organic acids extracted amount: non-volatile organic acids extracted amount is with the time of process
Extend and raise, when treated between when reaching 12min, non-volatile organic acids extracted amount reaches the highest, and the most non-volatile have
The prolongation over time of machine acid extracted amount and constantly decline.
Considering above four factors, greatest measured value to be obtained, optimal treatment conditions are as follows: sulfuric acid-methanol is molten
Liquid concentration is 12%；Sample is 1:30 with the mass volume ratio of sulfuric acid-methanol solution, processing pressure 700MPa；The process time
12min。
In the mensuration Nicotiana tabacum L. of the present invention, non-volatile organic acids is carried out at normal temperatures, and finding speed is fast, and side reaction does not occur,
Measured value is close to theoretical value, reproducible, and reliability is high.
Detailed description of the invention:
Embodiment 1
A kind of measuring the method for non-volatile organic acids in Nicotiana tabacum L., the non-volatile organic acids related generally to is the most following
One or a combination set of several: oxalic acid, malonic acid, succinic acid, lauric acid, positive ficocerylic acid, malic acid, positive 15 carbonic acid, Petiolus Trachycarpi
Acid, citric acid, stearic acid, oleic acid, linoleic acid, linolenic acid；Described Nicotiana tabacum L. specifically includes following one or a combination set of several: white
Rib Nicotiana tabacum L., cured tobacco leaf, atomatic tobacco, cabo, thin slice, expanded cut tobacco, blended type cigarette tobacco shred and Virginian-type cigarette tobacco shred；Institute
State the method specific requirement of non-volatile organic acids in mensuration Nicotiana tabacum L. as follows:
First extract tobacco sample and obtain corresponding treatment fluid, extract process as follows: by tobacco sample (because there being ratio below
Example, so need not volume mass here) pulverize be placed on food stage two-layer compound bag (be not required to limit content here, logical
Often the most so write in paper) in, adding the sulfuric acid-methanol solution that concentration is 10%, every 1.0000g tobacco sample adds
Sulfuric acid-methanol solution 25-50mL；Every 1.0000g tobacco sample adds the adipic acid inner mark solution 1mL of concentration 2.0mg/mL；
Sample is 1:30 with the mass volume ratio of sulfuric acid-methanol solution；Then two-layer compound bag is placed on supertension through hot-seal
Processing intracavity, through 700MPa pressure treatment to complete the esterification of non-volatile organic acids, the process time is that 12min obtains cigarette
The ultra high pressure treatment liquid of grass sample；
Then take ultra high pressure treatment liquid 12mL in 125mL separatory funnel, add the saturated NaCl solution of 20-30mL, every time
Extract with 10mL dichloromethane, extract 6 combining extraction liquids, add 3.5g anhydrous sodium sulfate and be dried overnight；
Use gas chromatogram to be measured extract afterwards, take the maximum recorded, in order to confirm in Nicotiana tabacum L. non-volatile
The content of property organic acid.
Measuring non-volatile organic acids in Nicotiana tabacum L. described in the present embodiment to carry out at normal temperatures, finding speed is fast, and pair does not occur
Reaction, measured value is close to theoretical value, reproducible, and reliability is high.
Embodiment 2
The method of non-volatile organic acids in described mensuration Nicotiana tabacum L., specific requirement is as follows:
1. material and instrument: sulphuric acid (analytical pure, Kaifeng Fang Jing chemical reagent company limited)；(purity is more than methanol
99.9%, Tianjin Siyou Fine Chemicals Co., Ltd.)；(purity is more than 99.8% to adipic acid, and China sends Buddhist nun's chemistry to try in Zhengzhou
Agent factory)；Oxalic acid (purity is more than 99.5%, Kaifeng chemical reagent head factory)；(purity is more than 98% to malonic acid, upper seamount Pu
Learn company limited)；Succinic acid (purity is more than 99.5%, Shanghai Pharmaceutical Group Solution on Chemical Reagents in Shanghai company)；(purity is big for lauric acid
In 99.5%, ACROS)；Positive ficocerylic acid (purity is more than 99.9%, MERCK)；(purity is more than 99.5% to malic acid, in China
Sea Pudong chemical reagent factory)；Positive 15 carbonic acid (purity is more than 99%, ACROS)；(purity is more than 99.5% to Palmic acid, middle traditional Chinese medical science
Solution on Chemical Reagents in Shanghai company of medicine group)；Citric acid (purity is more than 99.5%, Tianjin chemical reagent three factory)；Stearic acid (is analyzed
Pure, Solution on Chemical Reagents in Shanghai company of Chinese Medicine group)；Oleic acid (purity is more than 99%, Tokyo HuaCheng Industry Co., Ltd)；Sub-oil
Acid (purity is more than 99%, ACROS)；Linolenic acid (purity is more than 99.85%, lark prestige Science and Technology Ltd.)；Dichloromethane (point
Analyse pure, Tianjin De En chemical reagent company limited)；Anhydrous sodium sulfate (analytical pure, Tianjin De En chemical reagent company limited)；Chlorine
Change sodium (analytical pure, Tianjin De En chemical reagent company limited)；Nicotiana tabacum L. (Zhaotong County, Yunnan X2F)；
UHP900 × 2 ultra-high pressure apparatus (packet header, Inner Mongol Wen Tian Science and Technology Ltd.), the effective volume of supertension is 2L；
EL204 type electronic balance；Gas chromatograph instrument (GC7820A, Agilent company of the U.S.)
2. pretreatment of raw material: according to China's tobacco business standard YC/T31-1996 (Nicotiana tabacum L. and the preparation of tobacco product sample
With determination of water Oven Method) measure tobacco moisture percentage and prepare offal.Cigarette sample pulverizer is pulverized, crosses 40 mesh sieves and be placed on close
Close in container, use oven method measuring moisture content.
3. the preparation of standard solution: accurately weigh 5.9724g adipic acid in 1000mL volumetric flask, by methanol constant volume extremely
Scale, every milliliter of 5.9724mg Han adipic acid, this solution is inner mark solution.Accurately weigh 0.5024g oxalic acid；0.0807g the third two
Acid；0.0185g succinic acid；0.0143g lauric acid；The positive ficocerylic acid of 0.0167g；1.5153g malic acid；0.0123g positive 15
Carbonic acid；0.1460g Palmic acid；0.2976g citric acid；0.0516g stearic acid；0.04430g oleic acid；0.1072g linoleic acid；
0.1226g linolenic acid is in 50mL volumetric flask, with methanol constant volume to scale, every milliliter of 10.048mg Han oxalic acid；Malonic acid
1.614mg；Succinic acid 0.370mg；Lauric acid 0.286mg；Positive ficocerylic acid 0.334mg；Malic acid 30.306mg；Positive 15 carbon
Acid 0.246mg；Palmic acid 2.920mg；Citric acid 5.952mg；Stearic acid 1.032mg；Oleic acid 0.886mg；Linoleic acid
2.144mg；Linolenic acid 2.452mg, this solution is organic acid standard mother solution.
4. the configuration of sulfuric acid-methanol solution
Ice-cold and stirring under conditions of, respectively by 20mL, 40mL, 60mL, 80mL, 10mL, 120mL, 150mL concentrated sulphuric acid
It is slowly added in 980mL, 960mL, 940mL, 920mL, 900mL, 880mL, 850mL methanol, shakes up, then move into 2000mL
In reagent bottle, save backup.
The drafting of standard curve: take respectively polyprotic acid Standard Stock solutions 0.2mL, 0.5mL, 1.0mL, 2.0mL, 3.0mL,
In the two-layer compound bag that 4.0mL, 5.0mL, 6.0mL are placed in, each addition 1.0mL adipic acid inner mark solution and 40mL10% sulphuric acid first
Alcoholic solution, two-layer compound bag is placed on ultra high pressure treatment intracavity, processing pressure 500MPa through hot-seal, processes time 12min.
Take treatment fluid 10mL in 125mL separatory funnel, add the mixing of 20mL distilled water, extract with 10mL dichloromethane every time, extract 6
Secondary combining extraction liquid, adds 3.5g anhydrous sodium sulfate and is dried overnight.Take appropriate extract in chromatograph bottle, then chromatogram bottle is put
It is placed in the automatic sampler of Agilent7820A gas chromatograph and carries out gas chromatographic analysis.With each polyprotic acid respective area with
The ratio of internal standard respective area is abscissa, draws standard curve with each polyprotic acid quality and internal standard mass ratio for vertical coordinate,
Working curve.Oxalic acid regression equation is y=3.251x+0.063, correlation coefficient 0.999；Malonic acid regression equation is y=1.839x+
0.003, correlation coefficient 0.999；Succinic acid regression equation is y=1.256x+0.002, correlation coefficient 0.999；Lauric acid recurrence side
Journey is y=0.636x+5E-05, correlation coefficient 0.999；Positive ficocerylic acid regression equation is y=0.623x-6E-05, correlation coefficient
0.999；Malic acid regression equation is y=2.276x+0.375, correlation coefficient 0.999；Positive 15 carbonic acid regression equations are y=
0.620x+0.000, correlation coefficient 0.999；Palmic acid regression equation is y=0.597x+0.012, correlation coefficient 0.999；Fructus Citri Limoniae
Acid regression equation is y=1.754x+0.032, correlation coefficient 0.999；Stearic acid regression equation is y=0.749x+0.005, relevant
Coefficient 0.999；Oleic acid regression equation is y=0.847x+0.007, correlation coefficient 0.999；Linoleic acid regression equation is y=0.640x
+ 0.044, correlation coefficient 0.999；Linolenic acid regression equation is y=0.806x+0.110, correlation coefficient 0.997.Equation linear
Well, the quantitative analysis of nonvolatile organic acids in Nicotiana tabacum L. is suitable to.
5. the mensuration of non-waving property organic acid in Nicotiana tabacum L.: accurately weigh in the two-layer compound bag that about 1.0000g sample is placed in, add
The volume entering sulfuric acid-methanol solution is 25-40mL, and sulfuric acid-methanol solution concentration is 10-15%, adds 1mL adipic acid internal standard
Molten, two-layer compound bag is placed on ultra high pressure treatment intracavity through hot-seal, and through 600-900MPa pressure treatment, the process time is
10-15min.Take treatment fluid 10mL in 125mL separatory funnel, add the saturated NaCl solution of 20mL, use 10mL dichloromethane every time
Alkane extracts, and extracts 6 combining extraction liquids, adds 3.5g anhydrous sodium sulfate and is dried overnight.Gas chromatogram is used to carry out extract
Measure.Gas Chromatographic Determination result is carried out quantitatively by application Internal standard curve method.
6. analytical conditions for gas chromatography
Chromatographic column: HP-INNOWAX (30m × 0.25mm × 0.25 μm)；Carrier gas: helium；Column flow rate: 1.2mL/min(is permanent
Stream mode)；Average linear velocity: 29.134cm/sec；Injector temperature: 280 DEG C；Split ratio: 5:1；Temperature programming: at the beginning of chromatographic column
Beginning temperature 50 C (keeping 2min) is warming up to 220 DEG C (keeping 20min) with 4 DEG C/min；Sample size: 1.0 μ L.
Detector condition: detector: FID；Detector temperature: 280 DEG C；Hydrogen: 40mL/min；Air: 450mL/min；
Tail blows: 20mL/min.
The present embodiment relative to prior art, advantage is: carry out under non-volatile organic acids room temperature in Nicotiana tabacum L. processing, being difficult to
Side reaction occurs, and the response time is short, and measured value is stable.
Embodiment 3
In described mensuration Nicotiana tabacum L., the method for non-volatile organic acids, outside specific requirement reference example 1,2, the most following
Require: accurately weigh in the two-layer compound bag that about Yunnan Flue-cured Tobacco C3F1.0000g sample is placed in, add the body of sulfuric acid-methanol solution
Amassing as 35mL, sulfuric acid-methanol solution concentration is 12.0%, adds 1mL adipic acid internal standard molten, and two-layer compound bag is through hot-seal
Being placed on ultra high pressure treatment intracavity, through 750MPa pressure treatment, the process time is 12min.Take treatment fluid 10mL in 125mL separatory
In funnel, add the saturated NaCl solution of 20mL, extract with 10mL dichloromethane every time, extract 6 combining extraction liquids, add 5.0g
Anhydrous sodium sulfate is dried overnight.Gas chromatogram is used to be measured extract.Application Internal standard curve method is to gas chromatogram
Measurement result carries out quantitatively, and measured value is 162.61mg/g.
Embodiment 4
In described mensuration Nicotiana tabacum L., the method for non-volatile organic acids, outside specific requirement reference example 1,2, the most following
Require: accurately weigh in the two-layer compound bag that Henan flue cured tobacco cabo 1.1000g sample is placed in, add the body of sulfuric acid-methanol solution
Amassing as 30mL, sulfuric acid-methanol solution concentration is 11.5%, adds 1mL adipic acid internal standard molten, and two-layer compound bag is through hot-seal
Being placed on ultra high pressure treatment intracavity, through 720MPa pressure treatment, the process time is 12min.Take treatment fluid 10mL in 125mL separatory
In funnel, add the saturated NaCl solution of 25mL, extract with 10mL dichloromethane every time, extract 6 combining extraction liquids, add 5.0g
Anhydrous sodium sulfate is dried overnight.Gas chromatogram is used to be measured extract.Application Internal standard curve method is to gas chromatogram
Measurement result carries out quantitatively, and measured value is 126.81mg/g.
Embodiment 5
In described mensuration Nicotiana tabacum L., the method for non-volatile organic acids, outside specific requirement reference example 1,2, the most following
Requiring: accurately weigh in the two-layer compound bag that reconstituted tobacoo 1.1000g sample is placed in, the volume adding sulfuric acid-methanol solution is
35mL, sulfuric acid-methanol solution concentration is 11%, adds 1mL adipic acid internal standard molten, and two-layer compound bag is placed on through hot-seal
Ultra high pressure treatment intracavity, through 720MPa pressure treatment, the process time is 15min.Take treatment fluid 10mL in 125mL separatory funnel
In, add the saturated NaCl solution of 25mL, extract with 10mL dichloromethane every time, extract 6 combining extraction liquids, add 5.0g anhydrous
Sodium sulfate is dried overnight.Gas chromatogram is used to be measured extract.Application Internal standard curve method is to gas Chromatographic Determination
Result carries out quantitatively, and measured value is 138.56mg/g.
Embodiment 6
In described mensuration Nicotiana tabacum L., the method for non-volatile organic acids, outside specific requirement reference example 1,2, the most following
Require: accurately weigh in the two-layer compound bag that Virginian-type cigarette tobacco shred 1.0000g sample is placed in, add sulfuric acid-methanol solution
Volume is 30mL, and sulfuric acid-methanol solution concentration is 12.5%, adds 1mL adipic acid internal standard molten, and two-layer compound bag is through heat-sealing
Mouth is placed on ultra high pressure treatment intracavity, and through 750MPa pressure treatment, the process time is 12min.Take treatment fluid 10mL to divide in 125mL
In liquid funnel, add the saturated NaCl solution of 20mL, extract with 10mL dichloromethane every time, extract 6 combining extraction liquids, add
3.5g anhydrous sodium sulfate is dried overnight.Gas chromatogram is used to be measured extract.Application Internal standard curve method is to gas phase
Chromatographic determination result carries out quantitatively, and measured value is 165.37mg/g.
Embodiment 7
In described mensuration Nicotiana tabacum L., the method for non-volatile organic acids, outside specific requirement reference example 1,2, the most following
Requiring: accurately weigh in the two-layer compound bag that burley tobaccos 1.0000g sample is placed in, the volume adding sulfuric acid-methanol solution is
35mL, sulfuric acid-methanol solution concentration is 13.5%, adds 1mL adipic acid internal standard molten, and two-layer compound bag is rearmounted through hot-seal
In ultra high pressure treatment intracavity, through 800MPa pressure treatment, the process time is 13min.Take treatment fluid 10mL in 125mL separatory funnel
In, add the saturated NaCl solution of 25mL, extract with 10mL dichloromethane every time, extract 6 combining extraction liquids, add 5.0g anhydrous
Sodium sulfate is dried overnight.Gas chromatogram is used to be measured extract.Application Internal standard curve method is to gas Chromatographic Determination
Result carries out quantitatively, and measured value is 185.69mg/g.
Claims (1)
1. measuring a method for non-volatile organic acids in Nicotiana tabacum L., the non-volatile organic acids related generally to is specially following several
Kind one or a combination set of: oxalic acid, malonic acid, succinic acid, lauric acid, positive ficocerylic acid, malic acid, positive 15 carbonic acid, Palmic acid,
Citric acid, stearic acid, oleic acid, linoleic acid, linolenic acid；Described Nicotiana tabacum L. specifically includes following one or a combination set of several: white rib
Nicotiana tabacum L., cured tobacco leaf, atomatic tobacco, cabo, thin slice, expanded cut tobacco, blended type cigarette tobacco shred and Virginian-type cigarette tobacco shred；It is special
Levy and be: in described mensuration Nicotiana tabacum L., the method specific requirement of non-volatile organic acids is as follows:
First extract tobacco sample and obtain corresponding treatment fluid, extract process as follows: tobacco sample is pulverized and is placed on food stage
In two-layer compound bag, adding the sulfuric acid-methanol solution that concentration is 10%, every 1.0000g tobacco sample adds sulfuric acid-methanol solution
25-50mL；Every 1.0000g tobacco sample adds the adipic acid inner mark solution 1mL of concentration 2.0mg/mL；Then to two-layer compound
Bag is placed on ultra high pressure treatment intracavity through hot-seal, through 700MPa pressure treatment to complete the methyl ester of non-volatile organic acids
Changing, the process time is the ultra high pressure treatment liquid that 12min obtains tobacco sample；
Then take ultra high pressure treatment liquid 12mL, add the saturated NaCl solution of 20-30mL, extract with 10mL dichloromethane, extraction every time
Take 6 combining extraction liquids, add 3.5g anhydrous sodium sulfate and be dried overnight；
Use gas chromatogram to be measured extract afterwards, take the maximum recorded, in order to non-volatile in confirmation Nicotiana tabacum L. have
The content of machine acid.
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