CN104342364A - A rapid detector for pesticide residue detection and heavy metal detection - Google Patents
A rapid detector for pesticide residue detection and heavy metal detection Download PDFInfo
- Publication number
- CN104342364A CN104342364A CN201310313626.3A CN201310313626A CN104342364A CN 104342364 A CN104342364 A CN 104342364A CN 201310313626 A CN201310313626 A CN 201310313626A CN 104342364 A CN104342364 A CN 104342364A
- Authority
- CN
- China
- Prior art keywords
- detection
- heavy metal
- residual
- detector
- agriculture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
- C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A rapid detector for pesticide residue detection and heavy metal detection is disclosed. The rapid detector comprises a reagent bag, a detection card, and a card reading instrument. A reagent in the reagent bag is acetylcholinesterase. The detection card is provided with two detection holes. The card reading instrument comprises an image collecting system, an image processor, a display and control system, a data outputting port and a power supply system. The detector is advantageous in that: (1) the detector is simple in operation, and can be operated without professional training; (2) an accurate result can be obtained rapidly in 20 min; (3) a plurality of pesticide residues and heavy metals can be detected, and the detector is high in broad spectrum and high in detection sensitivity; and (4) data can be outputted, printed or loaded through USB, and is convenient in management.
Description
Technical field
The present invention relates to the technical field of Detecting Pesticide on tap water and the food such as fruit or vegetables, particularly relate to the residual and heavy metal analysis fast detector of a kind of agriculture.
Background technology
Pesticide residue remain in the general name of derivative, metabolite, agricultural chemicals parent, degradation product and impurity in environment, organism and food after referring to Pesticide use.The main agricultural chemicals causing vegetable pesticide residue amount to exceed standard is some countries organophosphorus pesticide of forbidding using in Vegetable produce and carbamate chemicals for agriculture, as thiophos, acephatemet, phorate, omethoate, parathion-methyl etc.The vegetables that edible pesticide residue exceed standard, very serious to the harm of human body, easily cause acute poisoning, even dead.
Control pesticide residue to the harm of human body, one of effective means is the dynamics strengthening detecting Pesticide Residues the most.Of a great variety for Fast Determination of Pesticide Residue method in the world, study carefully its principle and be mainly divided into two large classes: high efficiency chromatography fast detection method and biochemical assay.High performance liquid chromatography can carry out comparatively accurate detection by quantitative to most of agricultural chemicals, but consuming time oversize, and testing cost is too high, limits its development.Biochemistry detection method is whether the biochemical reaction utilizing certain biochemical substances extracted in organism to carry out exists and pesticidal contamination situation to judge pesticide residue, and when measuring, sample is without the need to passing through purification, or purifies fairly simple, and detection speed is fast.Be most widely used with inhibiting AChE and euzymelinked immunosorbent assay (ELISA) again in biochemistry detection method.
Enzyme inhibition rate method make use of the specific binding principle of Pseudocholinesterase to Organophosphorus and carbamate pesticides class agricultural chemicals, and therefore detection limit is very low.Be listed in national proposed standard method (GB/T 5009.199-2003).Because enzyme inhibition rate method has the features such as quick, sensitive, easy and simple to handle, with low cost, become one of mainstream technology of Organophosphorus and carbamate pesticides pesticide residue in fruits and vegetables being carried out to the detection of on-the-spot fast qualitative primary dcreening operation, obtained and apply more and more widely.There is many defects, such as complex operation in the existing residual detector of the agriculture of enzyme inhibition rate method that utilizes, detected result can not draw at once, or detected result is inaccurate etc.
Summary of the invention
The object of the present invention is to provide the residual and heavy metal analysis fast detector of a kind of agriculture of convenient operation.
For realizing above object, the present invention discloses following technical scheme: the residual and heavy metal analysis fast detector of a kind of agriculture, it is characterized in that, comprise pack, test card and Card Reader instrument, in described pack, reagent is acetylcholinesterase, described test card there are two test hole positions, described Card Reader instrument comprises image capturing system, image processor, display and Controlling System, data output and power-supply system, image capturing system is connected with image processor, and image processor is connected with data output, display and Controlling System and power-supply system.
As a preferred version, described test card is two.
As a preferred version, described image capturing system is digital camera, and described image processor is micro-chip or PC, and described display and Controlling System are liquid crystal touch display screen.
Organophosphate and carbamate insecticides are the sterilants that China mainly uses at present, are also two insecticides stronger to humans and animals toxicity in agricultural chemicals simultaneously.Both are inhibited to the acetylcholinesterase (AChE) decomposing neurotransmitter vagusstoff, the Decomposition of this enzyme can not normally be carried out, thus cause the accumulation of neurotransmitter acetylcholine, have impact on normal nerve conduction, even cause the poisoning or dead of organism.
According to this principle, be used in the detection to pesticide residue, by measuring vegetables extracting solution to after the suppression of AchE activity, enzyme is to the hydrolysis ability of its substrate, characterize its suppression situation to enzymic activity, thus judge wherein whether containing exceeding the Organophosphorus and carbamate pesticides class agricultural chemicals allowing residual content.
If it is lower not contain organophosphorus or carbamate pesticide residue or residual quantity in sample, the activity of enzyme would not be suppressed, and the substrate added in test is just hydrolyzed by enzymic hydrolysis or small part, and hydrolysate and the developer added react and produce color; Otherwise if wherein containing a certain amount of (exceeding detection limit) organophosphorus or carbamate chemicals for agriculture, the activity of enzyme will be suppressed, the substrate added in test just can not be hydrolyzed, thus do not develop the color or colour-change very little.With spectrophotometric determination light absorption value over time, calculate inhibiting rate, just can containing the residual condition of organophosphorus or carbamate chemicals for agriculture in judgement sample.
The invention has the advantages that: (1) this detector is simple to operate, can operate without the need to professional training; Within (2) 20 minutes, obtain accurate result fast; (3) can survey the residual and heavy metal substance of multiple agriculture, broad spectrum is high, and detection sensitivity is high; (4) data are exportable, can print or be downloaded by USB, convenient management.
Accompanying drawing explanation
Fig. 1 is the structural representation of Card Reader instrument.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further.The experimental technique used in following embodiment if no special instructions, is ordinary method.Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.
Residual and the heavy metal fast detector of embodiment 1. agriculture
Residual and the heavy metal analysis fast detector of agriculture, comprise pack, test card and Card Reader instrument, in described pack, reagent is acetylcholinesterase, and described test card has two, has two test hole positions on each, described Card Reader instrument comprises image capturing system, image processor, display and Controlling System, data output and power-supply system, image capturing system is connected with image processor, and image processor is connected with data output, display and Controlling System and power-supply system.Described image capturing system is digital camera, and described image processor is micro-chip or PC, and described display and Controlling System are liquid crystal touch display screen.
Whether the residual and heavy metal analysis fast detector of agriculture of the present invention adopts color reaction to carry out sample residual and heavy metal interpretation containing agriculture, but when carrying out a large amount of sample detection in practical application, judge as adopted eye-observation, can cause the deviation that testing staff is tired and then cause result to judge, therefore this agriculture of a kind of cooperation of design is residual and the special Card Reader instrument of heavy metal analysis fast detector.This instrument can carry out result interpretation to the residual test card of agriculture within 1 minute.
First the stationary installation corresponding with test card structure is adopted, test card firmly can be fixed, be convenient to the interpretation carrying out result in mobile environment, again by the display image of digital camera collecting test card, BP nerual network technique is adopted to process collection image through image processor, judgement is compared to blank on test card and sample well color distortion, and interpretation goes out sample whether there is pesticide residue or heavy metal, adopt liquid crystal display display result, and by this liquid crystal display, optimum configurations and monitoring instrument are carried out to instrument, without the need to outside display input unit, instrument volume can be greatly reduced.This equipment is powered and is adopted internal battery and external source, can select according to actual needs, and as adopted civil power in indoor, as adopted internal battery at the scene, sentence read result exports to print or be saved in computer and upload network by high speed USB mouth to be shared.The all parts of this equipment connect employing quick plug-in data line and connect, and are convenient to field maintemance.
Embodiment 2. is to the detection of the dish leaf of instillation fenitrothion 95, Malathion, SD-1750
One, detect
1, to the detection of the dish leaf of instillation fenitrothion 95
(1) a small pieces Chinese cabbage leaf sample is placed in dixie cup, takes out 400 μ L fenitrothion 95 standard specimens and drop on dish leaf.
(2) dixie cup is placed on ventilation until the fenitrothion 95 agricultural chemicals volatilization of blade surface is near dry.
(3) be put in pressure-sealing zipper plastics bag, add the pure water of 10mL.Dish leaf does not need chopping.
(4) pull on resealable zipper, within 10 minutes, be eluted to vegetable surface agricultural chemicals with hand rolling.
(5) open pack, reagent and two panels test card are placed on operator's console.Open Card Reader instrument.
(6) elutriant handled well in the sealed bag of 1mL is carefully drawn from container, in order to avoid be drawn to impurity with the 1mL Dispette configured in pack.
(7) water of 1mL negative control is added in two kinds of different reagent bottles, 1mL testing sample is added in two other reagent bottle.
(8) cover reagent bottle cap, fully rocked for 10 seconds up and down, mix until dissolve.
(9) reagent bottle is put back to worktable, incubation 30 minutes.This step can carry out timing by the timing key on reader screen.
After (10) 30 minutes, with disposable dropper, the reacted testing sample of 100 μ L in reagent bottle is transferred in the aperture of test card.
(11) incubation 15 minutes, carrys out timing by the timing key on reader screen.
(12) after time is up, press the Card Reader key on reader screen, and test card is inserted card reader, again press Card Reader key, read result.
(13), after reading a slice test card, take out and more other a slice is inserted.Press the result that Card Reader key reads another one test card.
2, to the detection of the dish leaf of instillation Malathion
(1) a small pieces Chinese cabbage leaf sample is placed in dixie cup, takes out 8mL Malathion standard specimen and drop on dish leaf.
(2) dixie cup is placed on ventilation until the Malathion agricultural chemicals volatilization of blade surface is near dry.
(3) be put in pressure-sealing zipper plastics bag, add the pure water of 10mL.
Following steps are with above-mentioned (4)-(13).
3, to the detection of 1ppm SD-1750
(1) pack is opened.Two panels test card is placed on operator's console.Open Card Reader instrument.
(2) the 1ppm SD-1750 standard specimen of 1mL is carefully drawn from container with the 1mL Dispette configured in pack.
(3) following steps are with above-mentioned (7)-(13).
4, to the detection of the dish leaf of instillation SD-1750
(1) be placed in dixie cup by a small pieces Chinese cabbage leaf sample, the 1ppm SD-1750 standard specimen taking out 1mL drops on dish leaf.
(2) dixie cup is placed on ventilation until the SD-1750 agricultural chemicals volatilization of blade surface is near dry.
(3) be put in pressure-sealing zipper plastics bag, add the pure water of 10mL.
(4) following steps are above-mentioned with (4)-(13).
Two, experimental result and discussion
1, to the detection of the dish leaf of instillation fenitrothion 95
Agriculture of the present invention result that the is residual and reading of heavy metal analysis fast detector is used to be feminine gender.Reach a conclusion as sample is negative.Because surveyed fenitrothion 95 is not can within survey scope, so this agricultural chemicals cannot be detected.
2, to the detection of the dish leaf of instillation Malathion
Agriculture of the present invention result that the is residual and reading of heavy metal analysis fast detector is used to be feminine gender.Reach a conclusion as sample is negative.The detection of Malathion is limited to 5ppm, and after we process dilution, residual on dish leaf of this agricultural chemicals is about 8ppm.
3, to the detection of 1ppm SD-1750 standard specimen
Agriculture of the present invention result that the is residual and reading of heavy metal analysis fast detector is used to be the positive.Reach a conclusion as sample is positive.
4, to the detection of the dish leaf of instillation SD-1750
Agriculture of the present invention result that the is residual and reading of heavy metal analysis fast detector is used to be the positive.Reach a conclusion as sample is positive.When sample adds 10mL wash-out, the concentration dilution of SD-1750 has arrived 0.1ppm, and it is still within detectability.
Three, sum up:
It is residual that the sample containing fenitrothion 95 and Malathion this time detected does not detect the agriculture exceeding threshold value.Because it is not in detectable species range to the detection of fenitrothion 95.And Malathion is not detected, may be out of date due to agricultural chemicals, toxicity reduces, or deals with improperly in dilution, dilute excessive caused by.SD-1750 standard specimen and the sample containing SD-1750 standard specimen are all detected, illustrate that the target compound within fast inspection instrument sensing range and detection limit all can be detected.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. the residual and heavy metal analysis fast detector of an agriculture, it is characterized in that, comprise pack, test card and Card Reader instrument, in described pack, reagent is acetylcholinesterase, described test card has two test hole positions, described Card Reader instrument comprises image capturing system, image processor, display and Controlling System, data output and power-supply system, image capturing system is connected with image processor, and image processor is connected with data output, display and Controlling System and power-supply system.
2. the residual and heavy metal analysis fast detector of a kind of agriculture according to claim 1, it is characterized in that, described test card is two.
3. the residual and heavy metal analysis fast detector of a kind of agriculture according to claim 1, it is characterized in that, described image capturing system is digital camera.
4. the residual and heavy metal analysis fast detector of a kind of agriculture according to claim 1, it is characterized in that, described image processor is micro-chip or PC.
5. the residual and heavy metal analysis fast detector of a kind of agriculture according to claim 1, it is characterized in that, described display and Controlling System are liquid crystal touch display screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310313626.3A CN104342364A (en) | 2013-07-24 | 2013-07-24 | A rapid detector for pesticide residue detection and heavy metal detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310313626.3A CN104342364A (en) | 2013-07-24 | 2013-07-24 | A rapid detector for pesticide residue detection and heavy metal detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104342364A true CN104342364A (en) | 2015-02-11 |
Family
ID=52498829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310313626.3A Pending CN104342364A (en) | 2013-07-24 | 2013-07-24 | A rapid detector for pesticide residue detection and heavy metal detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104342364A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101042350A (en) * | 2007-04-20 | 2007-09-26 | 上海农业信息有限公司 | Pesticide residue detector and detection method thereof |
| US20080160556A1 (en) * | 2006-12-13 | 2008-07-03 | Industrial Technology Research Institute | Bioassay element and producing method thereof |
| CN101587092A (en) * | 2009-07-07 | 2009-11-25 | 左少华 | The portable electrochemical biosensor of fast detecting organophosphorus pesticide residue |
| CN201749086U (en) * | 2010-09-16 | 2011-02-16 | 上海市农业科学院 | Rapid test card for detecting pesticide residue |
| CN102103122A (en) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | Rapid detector and detection method for electrochemical pesticide residue |
-
2013
- 2013-07-24 CN CN201310313626.3A patent/CN104342364A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080160556A1 (en) * | 2006-12-13 | 2008-07-03 | Industrial Technology Research Institute | Bioassay element and producing method thereof |
| CN101042350A (en) * | 2007-04-20 | 2007-09-26 | 上海农业信息有限公司 | Pesticide residue detector and detection method thereof |
| CN101587092A (en) * | 2009-07-07 | 2009-11-25 | 左少华 | The portable electrochemical biosensor of fast detecting organophosphorus pesticide residue |
| CN201749086U (en) * | 2010-09-16 | 2011-02-16 | 上海市农业科学院 | Rapid test card for detecting pesticide residue |
| CN102103122A (en) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | Rapid detector and detection method for electrochemical pesticide residue |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4431034B2 (en) | Sample collection and testing system | |
| JP4467304B2 (en) | Sample collection and testing system | |
| US20130280698A1 (en) | Rapid multiplex lateral flow assay device | |
| CN102803959B (en) | System and methods for rapid identification and/or characterization of a microbial agent in a sample | |
| US20180275109A1 (en) | Detection of Pesticide Residues in Agriculture | |
| US10408809B1 (en) | Sampling and detection kit for chemical and biological materials | |
| CN104502617A (en) | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue | |
| CN101699283A (en) | Intelligent food safety detection system and detection method | |
| CN103149200A (en) | Atmospheric particulate comprehensive toxicity detection method based on luminous bacteria method | |
| CN205067321U (en) | Fast pesticide residue detection instrument | |
| Batley | Quality assurance in environmental monitoring | |
| CN102183396A (en) | Sample pretreatment method for detecting pesticide residue applicable to enzymatic inhibition method, and pesticide detection method and box | |
| CN201885974U (en) | Handheld quick tester for pesticide residue | |
| CN201788153U (en) | Pesticide residue detector integrated with micro electronic balance | |
| CN104342364A (en) | A rapid detector for pesticide residue detection and heavy metal detection | |
| Chen et al. | Real-time monitoring for bioaerosols—flow cytometry | |
| CN202404017U (en) | Agriculture chemical residue instant detector | |
| CN102426167A (en) | Photoelectric conversion based rapid detection method for pesticide residual toxicity | |
| Ozanich et al. | Biodetection technologies for first responders: 2014 edition | |
| CN103674945A (en) | Organic phosphorus detection system and method | |
| CN102778454A (en) | Testing reagent for illegal cooking oil | |
| Baird et al. | Biodetection technologies for first responders | |
| CN201555840U (en) | Device for detecting safety of foods | |
| CN202204775U (en) | Pesticide residue fast detection card | |
| CN109580608A (en) | A kind of Rapid pesticide residue detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150211 |