CN104341463A - Monomer flavor Mal-Ile used for cigarettes as well as preparation method and application thereof - Google Patents
Monomer flavor Mal-Ile used for cigarettes as well as preparation method and application thereof Download PDFInfo
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- CN104341463A CN104341463A CN201410586132.7A CN201410586132A CN104341463A CN 104341463 A CN104341463 A CN 104341463A CN 201410586132 A CN201410586132 A CN 201410586132A CN 104341463 A CN104341463 A CN 104341463A
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- mal
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Abstract
The invention relates to a novel monomer flavor Mal-Ile used for cigarettes as well as a preparation method and application thereof. A structural formula of Mal-Ile is shown in the specification. Through physical humectant property tests of cigarettes and inner sensory quality evaluation of finished cigarettes, the monomer flavor is found to be capable of obviously improving the physical humectant properties of cut tobaccos, also have the function of obviously improving the sensory quality of the cigarettes and be capable of reducing the irritation and offensive odors of the cigarettes and improving the mellowness and fullness of the cigarette smoke.
Description
technical field
The invention belongs to essence and flavoring agent and tobacco manufacture field, be specifically related to a kind of Novel tobacco monomer perfume Mal-Ile, preparation method and application thereof.
background technology
Along with people are to the attention gradually of smoking and health problem, tar content in cigarette is while reducing further, and cigarette comfortableness is deteriorated, embody a concentrated reflection of moisture in cut tobacco scatter and disappear fast, main flume moisture is on the low side, pungency is bigger than normal, oral cavity is dry and phlegm is many etc.At present, in China's cigarette, a large amount of humectant used mainly contains propylene glycol, sorbyl alcohol etc., this type of humectant object is used to be improve the water ratio of pipe tobacco in the course of processing, improve pipe tobacco degrading resistance property, but above-mentioned humectant to the maintenance of cigarette rags water ratio and the improvement of sensory comfort unsatisfactory.
Summary of the invention
The object of the invention is to provide a kind of Novel tobacco monomer perfume Mal-Ile, preparation method and application thereof.
For achieving the above object, the present invention adopts following technical scheme:
A kind of cigarette monomer perfume Mal-Ile, its structural formula is as follows:
。
The preparation method of described cigarette monomer perfume Mal-Ile, it comprises the steps:
After maltose, anhydrous methanol and dimethyl formamide or mixed with propylene glycol, reflux 20-45min under 76-89 DEG C of condition, then add Isoleucine again to reflux 20-45min, finally add strong-acid ion exchange resin and kaempferol back flow reaction 2-8h; After reaction terminates, be separated and recrystallization and get final product through silica gel column chromatography; Wherein, feed ratio adds 0.138-0.396 gram of Isoleucine, 0.249-1.245 gram kaempferol, 0.035-0.068 gram strong-acid ion exchange resin, 1.7-2.8mL anhydrous methanol, 1.5-3.9mL dimethyl formamide or propylene glycol meter according to every 1 gram of maltose.
Concrete, column chromatography for separation elutriant used is the mixed solution of propyl carbinol, glacial acetic acid and water composition, and the volume ratio of three is 6:1:1, and developer is ethanol solution of ninhydrin.
Described Mal-Ile is as the application of fragrance-enhancing tobacco humectant in cigarette.
The synthetic route of the present invention's design is as follows:
。
The compounds of this invention Mal-Ile is a kind of aroma compounds of diving, its stable in properties under normal temperature, for the tasteless crystalline substance of white, but it produces tool by heating cleavable and is significantly increased the material of the fragrant and sweet of tobacco and baking perfume as furanone, ketene compounds, in addition, itself contain a large amount of hydrophilic radical, this compound is still rare at present as fragrance-enhancing tobacco humectant.
compared to the prior art, beneficial effect of the present invention:
1, the present invention is starting raw material with maltose first, is catalyzer, take kaempferol as inhibitor, react with Isoleucine with solid acid, successfully synthesizes a kind of Studies of The Aromatic Substances-Mal-Ile that dives, studies and determine synthesis technology condition and preferred plan.The method technique is simple, raw material is easy to get, productive rate is higher, is applicable to suitability for industrialized production.
2, the present invention develops a kind of Novel tobacco monomer perfume, first Mal-Ile is applied in cigarette as fragrance-enhancing tobacco humectant, by the test of cigarette physics humid keeping performance and the inherent sensory quality assessment of cigarette, result shows that it has excellent physics humectation effect to pipe tobacco, and the pungency of cigarette and assorted gas can be reduced, improve the mellow and full sense of cigarette smoke and comfortableness.
3, advantage of the present invention: according to existing document analysis, the synthesis about Mal-Ile has and rarely has report, and this route has the advantages such as raw material is easy to get, easy and simple to handle, productive rate significantly improves.Target product of the present invention due to obvious to the action effect of tobacco product, the bright prospects that therefore there is significant industrial application value He apply.
Accompanying drawing explanation
Fig. 1 is the infrared spectrogram of target product Mal-Ile;
Fig. 2 is the high resolution mass spectrum figure of target product Mal-Ile;
Fig. 3 is the carbon-13 nmr spectra figure of target product Mal-Ile.
Embodiment
Below by way of specific embodiment, the present invention is described in detail, but protection scope of the present invention is not limited thereto.
Be below portion of reagent used and instrument in embodiment.
Maltose, Isoleucine (99%, Aladdin chemical reagents corporation); Kaempferol (HPLC level, French Extrasynthese company); Anhydrous methanol, dehydrated alcohol, dimethyl formamide, propylene glycol, propyl carbinol, glacial acetic acid (AR, Tianjin Kai Tong chemical reagent company limited); Amberlite IR-120 (H) ion exchange resin (Alfa Aesar); Silica gel (chemical pure, Qingdao Marine Chemical Co., Ltd.).
Thermo Nicolet Avatar370 infrared spectrometer (Nicolet company of the U.S.); Bruker Avance AMX-400 nuclear magnetic resonance spectrometer (Bruker company of the U.S.); BS200SWE1 type electronic balance (sensibility reciprocal: 0.0001g, Beijing match Doris balance company limited); R-215 type Rotary Evaporators (BUCHI company of Switzerland); V-700 vacuum pump (BUCHI company of Switzerland); DLSB-20/60 DEG C of subcooling recycle pump (Shanghai Mei Qiang plant and instrument company limited); KDM type regulating temp. electrothermal cover (Shandong Hua Lu electric heating Instrument Ltd.).
embodiment 1
A preparation method of cigarette monomer perfume Mal-Ile, it comprises the steps:
1 gram of maltose, 1.5mL dimethyl formamide, 2.8mL anhydrous methanol is added in the there-necked flask with thermometer, prolong, reflux 20min under 76 DEG C of conditions, then add 0.138 gram of Isoleucine again to reflux 20min, finally add 0.035 gram of strong-acid ion exchange resin and 0.249 gram of kaempferol back flow reaction 2.0h; After reaction terminates, obtain white crystalline target product 0.395 gram through silica gel column chromatography separation, recrystallization.Wherein, column chromatography for separation elutriant used is the mixed solution of propyl carbinol, glacial acetic acid and water composition, and the volume ratio of three is 6:1:1, and developer is ethanol solution of ninhydrin; Recrystallization solvent for use is volume ratio is the dehydrated alcohol of 1:1 and the mixing solutions of anhydrous methanol.
embodiment 2
A preparation method of cigarette monomer perfume Mal-Ile, it comprises the steps:
1 gram of maltose, 3.6mL dimethyl formamide, 2.0mL anhydrous methanol is added in the there-necked flask with thermometer, prolong, reflux 35min under 85 DEG C of conditions, then add 0.256 gram of Isoleucine again to reflux 35min, finally add 0.053 gram of strong-acid ion exchange resin and 1.019 grams of kaempferol back flow reaction 6.0 h; After reaction terminates, obtain white crystalline target product 0.845 gram through silica gel column chromatography separation, recrystallization.Wherein, the operating process of column chromatography for separation and recrystallization is with embodiment 1.
embodiment 3
A preparation method of cigarette monomer perfume Mal-Ile, it comprises the steps:
1 gram of maltose, 3.9mL propylene glycol and 1.7mL anhydrous methanol is added in the there-necked flask with thermometer, prolong, reflux 45min under 89 DEG C of conditions, then add 0.396 gram of Isoleucine again to reflux 45min, finally add 0.068 gram of strong-acid ion exchange resin and 1.245 grams of kaempferol back flow reaction 8.0h; After reaction terminates, obtain white crystalline target product 0.584 gram through silica gel column chromatography separation, recrystallization.Wherein, the operating process of column chromatography for separation and recrystallization is with embodiment 1.
(1) structural characterization of target product
By infrared spectra (IR), high resolution mass spectrum (HR-MS), carbon-13 nmr spectra (
13c-NMR) carry out structural characterization to embodiment gained target product Mal-Ile respectively, the chemical structural formula of Mal-Ile is as follows:
。
1, infrared spectra
Adopt U.S. Nicolet company's T hermo Nicolet Avatar370 type infrared spectrometric analyzer to analyze to synthesized product, the results are shown in Figure 1.As seen from the figure, 3392.01cm
-1for the hydroxyl stretching vibration of associated state, describe the existence of hydroxyl, 1732.45cm
-1for carbonyl charateristic avsorption band.Tentatively can determine that this material and target product coincide thus.
2,
high resolution mass spectrum
Because this compound Polarity comparision is strong, and more responsive to ratio of specific heat, therefore adopt electric spray ion source (ESI) high resolution mass spec (HR-MS) to carry out mass spectroscopy (high resolution mass spectrometer Exactive Orbitrap, Thermo company of the U.S.).Solvent selects the methanol aqueous solution of 50% to be moving phase, the results are shown in Figure 2.
By to its positive ion mode map analysis, sample molecule quasi-molecular ions [M+H] can be obtained
+be 456.43464, hydrogenated molecule formula is C
18h
34nO
12, can judge that its quasi-molecule is C
18h
33nO
12, its relative molecular weight is 455.42680, and the Theoretical molecular formula of Mal-Ile is C
18h
33nO
12, its theoretical Mr 455.42631, therefore, sample molecule elementary composition is consistent with the elementary composition of Mal-Ile.According to N rule, containing odd number nitrogen-atoms in molecule, coincidence theory nitrogen-atoms number.
3,
carbon-13 nmr spectra
Fig. 3 is gained compound
13c-NMR composes, 18 the spectrum peaks provided in figure represent the carbon atom that in molecule, 18 kinds of chemical environments are different, according to spectral strength judge δ by low field to the letter of High-Field carbon atom number than being 1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1, its digital sum is 18, consistent with carbon atom number in Mal-Ile molecular formula.Tentatively carbon can be attributed to by figure: δ=172.680ppm is C
6 〞, δ=108.951ppm is C
1 ', δ=96.152ppm is C
2, δ=77.361ppm is C
4, δ=77.043ppm is C
5 ', δ=76.725ppm is C
2 ', δ=71.067ppm is C
3 ', δ=70.780ppm is C
4 ', δ=70.565ppm is C
5, δ=70.327ppm is C
3, δ=68.659ppm is C
6, δ=66.374ppm is C
6 ', δ=61.089ppm is C
1 〞, δ=59.783ppm is C
1, δ=37.986ppm is C
2 〞, δ=24.881ppm is C
3 〞, δ=15.833ppm is C
5 〞, δ=11.450ppm is C
4 〞.From the above mentioned, this
13c-NMR spectrum is coincide with the structure of target compound Mal-Ile.
In sum, in conjunction with infrared spectra (IR), high resolution mass spec (HR-MS) and carbon-13 nmr spectra (
13c-NMR) can determine that gained compound is Mal-Ile.
(2) applied research of target product in tobacco
1, the physics humid keeping performance test of target product
For investigating the physics humid keeping performance of target product, with blank cigarette for carrier, embodiment 2 gained target product and traditional tobacco humectant propylene glycol are carried out the research of physics humid keeping performance contrast test, first utilize the aqueous ethanolic solution of 50V% that target product Mal-Ile and propylene glycol are mixed with the solution that massfraction is 10% respectively, then evenly spray is added in blank pipe tobacco respectively, addition is 0.3% of pipe tobacco weight, and wherein blank pipe tobacco sprays into the aqueous ethanolic solution of the 50V% of equivalent.Then by kind of the tobacco sample of 3 after application of sample at relative humidity (60 ± 2) %, temperature (22 ± 1) DEG C conditional equilibrium 48h, then 4 parts of pipe tobaccos are respectively got, every part of 10g, wherein two parts are placed in relative humidity 45%, in temperature 22 DEG C of fixed temperature and humidity test experiments case test environments, other two parts are placed in relative humidity 85%, in temperature 22 DEG C of fixed temperature and humidity test experiments case test environments, every 6h weighs once, the change of instant water ratio is analyzed, investigate its physics humectation effect, according to the weight differential of initial weight and the equilibrium moisture content of initial aqueous rate calculation sample pipe tobacco, test result gets 2 Duplicate Samples mean values, test continues to pipe tobacco dehydration always or water suction reaches balance, the physics humid keeping performance effect of target product and propylene glycol sees the following form.
Test-results is known, and under the drying conditions of relative humidity 45%, temperature 22 DEG C, add the equilibrium moisture content of the pipe tobacco of target product and propylene glycol all higher than blank pipe tobacco, the physics humid keeping performance wherein adding Mal-Ile is best, is secondly propylene glycol; And under the condition of relative humidity 85%, temperature 22 DEG C, the pipe tobacco equilibrium moisture content of adding Mal-Ile, a little more than propylene glycol, shows that compound disclosed in this invention has good humectation effect to pipe tobacco.
2, the sense organ humid keeping performance test of target product
Make solvent with 20V% aqueous ethanolic solution, embodiment 2 gained target product (Mal-Ile) is made into the solution of massfraction 1%.Get the above-mentioned solution containing target product of 1.00g, 2.00g, 5.00g, 10.00g, 17.50g and 20.00g respectively, then evenly spray is added in the blank cigarette shreds of 100g of six parts of non-flavoring and casings, roll, respectively pick out 100 same weight cigarette, be placed in temperature 22 DEG C ± 1 DEG C, balance 48h in the climatic chamber of humidity 60% ± 2%, smoke panel test.Control sample is blank cigarette, and control sample balances 48h equally under same temperature, humidity condition.
Smoking result (see table 1) shows, the Main Function of target product to cigarette shows as and make flue gas obviously become mellow and full, soft, and comfortable taste promotes, and assorted gas and pungency reduce.When target product consumption is less, it is not clearly to cigarette effect; When its consumption is more than 1.75 ‰, occur that spine sense increases, the phenomenon that concentration reduces; When its consumption is 1.75 ‰, sucking quality is best.Therefore, best results when adding 0.175g target product in 100g cigarette, namely optimum consumption is 1.75 ‰.
The perfuming smoking result of table 1 Mal-Ile
Claims (3)
1. a cigarette monomer perfume Mal-Ile, its structural formula is as follows:
。
2. the preparation method of monomer perfume Mal-Ile of cigarette described in claim 1, is characterized in that, comprise the steps:
After maltose, anhydrous methanol and dimethyl formamide or mixed with propylene glycol, reflux 20-45min under 76-89 DEG C of condition, then add Isoleucine again to reflux 20-45min, finally add strong-acid ion exchange resin and kaempferol back flow reaction 2-8h; After reaction terminates, be separated and recrystallization and get final product through silica gel column chromatography; Wherein, feed ratio adds 0.138-0.396 gram of Isoleucine, 0.249-1.245 gram kaempferol, 0.035-0.068 gram strong-acid ion exchange resin, 1.7-2.8mL anhydrous methanol, 1.5-3.9mL dimethyl formamide or propylene glycol meter according to every 1 gram of maltose.
3. Mal-Ile described in claim 1 is as the application of fragrance-enhancing tobacco humectant in cigarette.
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| US3478015A (en) * | 1966-11-14 | 1969-11-11 | Yuki Gosei Yakuhin Kogyo Kk | Process for reacting amino acid and an active carbonyl sugar in a polyhydric alcohol |
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| CN104114176A (en) * | 2011-12-27 | 2014-10-22 | 森下仁丹株式会社 | Maillard reaction inhibitor |
-
2014
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Patent Citations (4)
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| US3478015A (en) * | 1966-11-14 | 1969-11-11 | Yuki Gosei Yakuhin Kogyo Kk | Process for reacting amino acid and an active carbonyl sugar in a polyhydric alcohol |
| CN101704850A (en) * | 2009-10-21 | 2010-05-12 | 安徽中烟工业公司 | Method for separating and extracting compound of Amadori of Maillard reaction midbody |
| CN104114176A (en) * | 2011-12-27 | 2014-10-22 | 森下仁丹株式会社 | Maillard reaction inhibitor |
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Address after: 450000 Yulin South Road, Henan, Zheng Dong, No. 16 South Road, Zhengzhou Applicant after: China Tobacco Henan Industrial Co., Ltd. Address before: 450000 Zhengzhou agricultural road, Henan, No. 29 Applicant before: China Tobacco Henan Industrial Co., Ltd. |
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