CN104341013A - Method for inhibiting excessive growth of algae in water body by using masson pine leaves - Google Patents
Method for inhibiting excessive growth of algae in water body by using masson pine leaves Download PDFInfo
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- CN104341013A CN104341013A CN201410064788.2A CN201410064788A CN104341013A CN 104341013 A CN104341013 A CN 104341013A CN 201410064788 A CN201410064788 A CN 201410064788A CN 104341013 A CN104341013 A CN 104341013A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 52
- 241000018650 Pinus massoniana Species 0.000 title claims abstract description 51
- 235000011609 Pinus massoniana Nutrition 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 230000002401 inhibitory effect Effects 0.000 title abstract description 9
- 239000000843 powder Substances 0.000 claims description 25
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 14
- 230000003203 everyday effect Effects 0.000 claims description 12
- 235000019687 Lamb Nutrition 0.000 claims description 8
- 241000192710 Microcystis aeruginosa Species 0.000 claims description 8
- 206010020880 Hypertrophy Diseases 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 7
- 230000005764 inhibitory process Effects 0.000 abstract description 3
- 239000008239 natural water Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 18
- 230000003287 optical effect Effects 0.000 description 8
- 230000001629 suppression Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 241000220317 Rosa Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 238000001311 chemical methods and process Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 241000192700 Cyanobacteria Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003619 algicide Substances 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 229920000592 inorganic polymer Polymers 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
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- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for inhibiting excessive growth of algae in a water body by using masson pine leaves. The method comprises the steps of crushing or grinding the fresh masson pine leaves into pulp and putting into the water body to be treated, wherein the dosage is 1.76-4g/L per day and dosing is performed for consecutive 3 days. The method provided by the invention is low in price, good in algal inhibition effect, rapid in onset of action, simple to operate and safe in ecology. The method can be applied to algal control of natural water bodies or artificial water bodies, such as lakes, reservoirs, rivers, park landscape water bodies, family landscape water bodies and the like. By adopting the method, the algal inhibition rate is 32%-70% against the water body with the initial density of the algae of 35000-100000/mL.
Description
Technical field
The present invention relates to a kind of method that Measures of Algae in Water Body controls, be specifically related to use Pinus massoniana to suppress the method for Measures of Algae in Water Body hypertrophy.
Background technology
In recent years, along with the fast development of society, trade effluent and sanitary sewage discharge in a large number, in agricultural area source, nitrogen, phosphorus seriously run off, a large amount of organic pollutants and nutritive salt directly or indirectly enter water body, cause body eutrophication trend to be on the rise, many water bodys are faced with the risk of outburst wawter bloom (water bloom).In China, modal is the microcystis waterbloom that is sociales with microcystic aeruginosa (Mirocystis aeruginosa), if the blue-green alga bloom of Taihu Lake, Chaohu, Dian Chi outburst is all based on microcystis waterbloom.How to remove and control the harmful algae such as Microcystis aeruginosa in water body excessive flourish, prevent and treat wawter bloom, be China and even whole world great environmental problem in science urgently to be resolved hurrily.
At present, the method controlling Measures of Algae in Water Body hypertrophy mainly contains physical method, chemical process and biological method.Physical method directly can remove the algae in water body, can not produce secondary pollution, but equipment and running cost high, labour intensity is comparatively large and can not continue the removal effect of algae.Published patented technology comprises " a kind of Multifunctional mobile being applicable to river channel type water body presses down removing algae platform " (publication number: CN203095654U), " multifunctional water surface algae removing machine " (publication number: CN201050078), " a kind of intensified by ultrasonic wave air-floating apparatus except algae and Algae toxins " (publication number: CN202214239U).Chemical process instant effect and successful, but owing to there is the possibility causing secondary pollution, determine the ecological impact of water surrounding is bad, life-time service can pollute water ecological setting.Published patented technology comprises, and " removing the method for blue-green algae in waters " (publication number: CN1762847) adopts wilkinite, polymerize aluminum chloride, the composite rear sprinkling of cationic-type polyacrylamide three kinds of flocculation agents, " a kind of composite type inorganic polymer stablizes preparation and the algae-removing method of algicide " (publication number: CN103030203A), the survive the winter minimizing technology (publication number: CN102295333A) of bloom blue algae provenance of large-scale shallow water lake adopts paddy stalk and the provenance of H2O2 to bloom blue algae of surviving the winter to remove.Biological method ecological security is good, but it is slow often to take effect, except algae effect affects larger by biological activity.Published patented technology comprises " having the active Pseudomonas aeruginosa of molten algae and the application in blue-green alga bloom controls " (publication number: CN101481670), " bristle algae and transparent flea associating algae control method " (publication number: CN101041505) etc.In recent years, emerging research field is become by natural biologic material processing, the research of making algae inhibiting product of purifying.
Containing abundant nutritive substance and biologically active substance in Folium Pini, as VITAMIN, hormone, bacteriocidin and enzyme etc.Folium Pini makes powder, is used for additive for farm animal feed.The result of Liu Zhen etc. " acute toxicity of From Pineneedles of Pinus Massoniana Lamb flour bag tea water extract and mutagenicity research " shows: the teabag aqueous extract of From Pineneedles of Pinus Massoniana Lamb powder is nontoxic, without mutagenesis [canceration distortion sudden change, 2012,24 (5)].The published patent relevant to pine tree of water treatment field has a kind of method for deodorizing wastewater (publication number: CN1962465) etc. of Folium Pini.
Summary of the invention
The object of this invention is to provide a kind of method using Pinus massoniana to suppress Measures of Algae in Water Body hypertrophy, the method is cheap, effect of algae restraint good, onset time is fast, simple to operate, ecological safety.
For achieving the above object, the present invention includes following technical scheme:
Use Pinus massoniana to suppress a method for Measures of Algae in Water Body hypertrophy, the method comprises: fresh Pinus massoniana is ground into powder or wears into juice and drop in staying water, adding dosage is 1.76-4g/L every day, adds at least 3 days continuously.
Method as above, preferably, the preparation method of described Pinus massoniana powder is: cleaned by fresh Pinus massoniana and dry, being ground into granularity with pulverizer is 40-200 object powder, through ultra violet lamp sterilizing.
Method as above, preferably, the dosage that adds of described Pinus massoniana powder is 2.4-4g/L every day, adds at least 3 days continuously.
Method as above, preferably, the method that described Pinus massoniana Lamb wears into juice is: cleaned by fresh Pinus massoniana, is milled to Pinus massoniana juice subsequently.
Method as above, preferably, the dosage that adds of described Pinus massoniana juice is 1.6-2mL/L every day, adds at least 3 days continuously.
Method as above, preferably, described Measures of Algae in Water Body concentration is 3.5 ~ 100,000/mL.
Method as above, preferably, described algae is Microcystis aeruginosa.
Method as above, preferably, described algae is microcystic aeruginosa.
Method as above, wherein, described Pinus massoniana is the needle of Pinaceae Pinus Pinus massoniana Lamb (Pinus Massoniana Lamb.).
Beneficial effect of the present invention is: utilize Pinus massoniana to carry out the suppression of harmful algae in water body, have cheap, effect of algae restraint good, onset time is fast, simple operation and other advantages.The present invention can be applicable to the algal inhibition of natural water or artificial water, as lake, reservoir, river, park landscape water body, family's landscape water body etc.Experimental result shows, be the water body of 3.5 ~ 100,000/mL for algae initial density, algal control rate is 32% ~ 70%.
Accompanying drawing explanation
Fig. 1 is that difference adds the Pinus massoniana powder of concentration to the suppression graphic representation compared with low density algae.
Fig. 2 is the suppression graphic representation of Pinus massoniana powder to higher density algae that difference adds concentration.
Fig. 3 is the suppression graphic representation of Pinus massoniana juice to algae that difference adds concentration.
Embodiment
Embodiment 1 Pinus massoniana powder is to the suppression compared with low density Microcystis aeruginosa
Cleaned by fresh Pinus massoniana and dry, being ground into granularity with pulverizer is 40-200 object powder, through ultra violet lamp sterilizing 30min, namely obtains Pinus massoniana powder.Get Pinus massoniana powder 0.8g, 0.64g and 0.48g respectively, adding 200mL initial density is in the microcystic aeruginosa nutrient solution of 20,000/mL.Every day 1 time, add 3 days continuously.Test group and control group respectively establish 3 bottles parallel.Add the 0-3 days of Pinus massoniana powder, measure the optical density(OD) under each bottle algae liquid 695nm every day.The 3rd day after adding Pinus massoniana Lamb powder, control group algae optical density(OD) rose 81%, and the algae optical density(OD) of each test group have dropped 33%, 26% and 5% respectively than during on-test.Compared with the algae density of control group, the algal control rate of test group reaches 70%, 66% and 53% respectively.
As shown in Figure 1, the change of curve representation control group and test group algae optical density(OD), compared with control group, after adding, the 3rd day each test group all shows significant alga-inhibiting action, and inhibiting rate is up to 70%.
Embodiment 2 Pinus massoniana powder is to the suppression of higher density Microcystis aeruginosa
Cleaned by fresh Pinus massoniana and dry, being ground into granularity with pulverizer is 40-200 object powder, and powder, through ultra violet lamp sterilizing 30min, namely obtains Pinus massoniana powder.Get Pinus massoniana powder 0.8g, 0.70g, 0.6g and 0.5g respectively, adding 200mL initial density is in the microcystic aeruginosa nutrient solution of 100,000/mL.Every day 1 time, add 3 days continuously.Test group and control group respectively establish 3 bottles parallel.Add the 0-3 days of Pinus massoniana powder, measure the optical density(OD) under each bottle algae liquid 695nm every day.The 3rd day after adding Pinus massoniana Lamb powder, control group algae optical density(OD) rose 23%, and the algae optical density(OD) of each test group have dropped 30%, 36%, 20% and 17% respectively than during on-test.Compared with the algae density of control group, the algal control rate of test group is respectively 41%, 48%, 35% and 31%.
As shown in Figure 2, the change of curve representation control group and test group algae optical density(OD), compared with control group, after adding, the 3rd day each test group all shows significant alga-inhibiting action, and inhibiting rate is up to 48%.
The algal control of embodiment 3 Pinus massoniana juice is tested
Cleaned by fresh Pinus massoniana, immersion grinds, and obtains Pinus massoniana juice.Pinus massoniana juice 0.4mL and 0.32mL being dropped into respectively 200mL initial density is in 3.5 ten thousand/mL microcystic aeruginosa nutrient solution.Every day 1 time, add 3 days continuously.Test group and control group respectively establish 3 bottles parallel.Add the 0-3 days of Pinus massoniana juice, measure the algae individual amount of each bottle algae liquid every day.The 3rd day after adding Pinus massoniana Lamb juice, control group phycobiont quantity rose 34%, and the algae individual amount of each test group have dropped 39% and 11% respectively than during on-test.Compared with the algae number of individuals of control group, the algal control rate of test group is respectively 54% and 32%.
As shown in Figure 3, the change of curve representation control group and test group algae individual amount, compared with control group, after adding, the 3rd day each test group all shows obvious alga-inhibiting action, and inhibiting rate is up to 54%.
Above embodiment is only for illustration of the present invention; and be not limitation of the present invention; the those of ordinary skill of relevant technical field; without departing from the spirit and scope of the present invention; can also make a variety of changes and replace; therefore all equivalent technical schemes also belong to category of the present invention, and scope of patent protection of the present invention should be defined by the claims.
Claims (8)
1. the method using Pinus massoniana to suppress Measures of Algae in Water Body hypertrophy, it is characterized in that, the method comprises: fresh Pinus massoniana is ground into powder or wears into juice and drop in staying water, adding dosage is 1.76-4g/L every day, adds at least 3 days continuously.
2. the method for claim 1, is characterized in that, the preparation method of described Pinus massoniana powder is: cleaned by fresh Pinus massoniana and dry, being ground into granularity with pulverizer is 40-200 object powder, through ultra violet lamp sterilizing.
3. method as claimed in claim 2, it is characterized in that, the dosage that adds of described Pinus massoniana powder is 2.4-4g/L every day, adds at least 3 days continuously.
4. the method for claim 1, is characterized in that, the method that described Pinus massoniana Lamb wears into juice is: cleaned by fresh Pinus massoniana, is milled to Pinus massoniana juice subsequently.
5. method as claimed in claim 4, it is characterized in that, the dosage that adds of described Pinus massoniana juice is 1.6-2mL/L every day, adds at least 3 days continuously.
6. the method for claim 1, is characterized in that, described Measures of Algae in Water Body concentration is 3.5 ~ 100,000/mL.
7. the method according to any one of claim 1-6, is characterized in that, described algae is Microcystis aeruginosa.
8. method as claimed in claim 7, it is characterized in that, described algae is microcystic aeruginosa.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410064788.2A CN104341013B (en) | 2013-12-17 | 2014-02-25 | Method for inhibiting excessive growth of algae in water body by using masson pine leaves |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310695463 | 2013-12-17 | ||
| CN201310695463X | 2013-12-17 | ||
| CN201310695463.X | 2013-12-17 | ||
| CN201410064788.2A CN104341013B (en) | 2013-12-17 | 2014-02-25 | Method for inhibiting excessive growth of algae in water body by using masson pine leaves |
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| Publication Number | Publication Date |
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| CN104341013A true CN104341013A (en) | 2015-02-11 |
| CN104341013B CN104341013B (en) | 2017-02-15 |
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| CN201410064788.2A Expired - Fee Related CN104341013B (en) | 2013-12-17 | 2014-02-25 | Method for inhibiting excessive growth of algae in water body by using masson pine leaves |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107117669A (en) * | 2017-06-16 | 2017-09-01 | 长沙鼎成新材料科技有限公司 | A kind of eutrophication water treatment agent and preparation method thereof |
| CN107857347A (en) * | 2017-10-30 | 2018-03-30 | 中南民族大学 | A kind of application of watermelon peel in Measures of Algae in Water Body undue growth is suppressed |
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2014
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107117669A (en) * | 2017-06-16 | 2017-09-01 | 长沙鼎成新材料科技有限公司 | A kind of eutrophication water treatment agent and preparation method thereof |
| CN107117669B (en) * | 2017-06-16 | 2020-08-14 | 上海永泉化工科技有限公司 | Eutrophic water treatment agent and preparation method thereof |
| CN107857347A (en) * | 2017-10-30 | 2018-03-30 | 中南民族大学 | A kind of application of watermelon peel in Measures of Algae in Water Body undue growth is suppressed |
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| CN104341013B (en) | 2017-02-15 |
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