CN104330375A - Method for measuring light stability of monascus red pigment - Google Patents
Method for measuring light stability of monascus red pigment Download PDFInfo
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- CN104330375A CN104330375A CN201410565033.0A CN201410565033A CN104330375A CN 104330375 A CN104330375 A CN 104330375A CN 201410565033 A CN201410565033 A CN 201410565033A CN 104330375 A CN104330375 A CN 104330375A
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- monascorubin
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Abstract
The invention relates to a method for measuring the light stability of a monascus red pigment, and belongs to the field of food additives and detection methods thereof. The method for measuring the light stability of the monascus red pigment comprises the following steps: separating commercially available powdered monascorubin or paste monascorubin to obtain a red pigment, a nacarat pigment and a yellow pigment, respectively dissolving the obtained pigments into distilled water to prepare a pigment solution with certain concentration, and measuring the initial absorbance of the distilled water serving as a blank by an ultraviolet visible spectrophotometer; putting a certain amount of the pigment solution into a plate with the diameter phi of 12cm, putting the plate under a 40W ultraviolet lamp for irradiation at the distance of 15cm, and taking out the plate to measure the absorbance every 20min. The method disclosed by the invention has the characteristics of high accuracy, high repetitiveness, simplicity and convenience in operation and the like.
Description
Technical field
The present invention relates to a kind of assay method of monascorubin light stability, belong to food additives and detection method field thereof.
Background technology
The light stability of monascorubin is not high, this is well-known, assay method for its light stability roughly has following several: one is the method adopting solar irradiation, configure certain density pigment solution and be placed in transparent glass container, then irradiate under being placed on sunshine, measure the look valency of pigment solution every certain hour, be the storage rate of pigment with postradiation look valency divided by the look valency of pre-irradiation.Measuring more accurately is solar energy is converted into langeys (500langleys is equivalent to sunlight in the summer energy of a day) to weigh exposure intensity.Two is the methods with ultra violet lamp, the container (test tube or beaker etc.) filling finite concentration pigment solution is positioned over distance uviol lamp (40W, certain distance (15cm 30w), 25cm) place is irradiated, measure liquid color valency at regular intervals, calculate look valency storage rate.Three is adjusted by the water-soluble red pigment of red rice be separated to absorbance to be 0.5 (500nm) in aqueous, is placed in Pyrextube, places under natural light, the change of absorbance under mensuration 500nm.The factor of five aspects is all related to above: radiation source, irradiation distance, pigment solution irradiated area, the absorbance of pre-irradiation pigment solution and irradiation time in all assay methods.In radiation source, sunshine and natural light are not reliable and stable light sources, and its light energy is different with climate change, has ultraviolet light luminous energy under certain power only more stable, and thus ultra violet lamp method mensuration monascorubin light stability is comparatively reliable.The relation of organism to the absorption of ultraviolet light and distance is very large.Too closely can not distinguish the difference of light stability between different monascorubin, minute too far away is oversize again, and be not easy to operation, according to bibliographical information, distance 15-25cm is more suitable.The ultraviolet light of glass Absorbable rod more than 90%, so above document transparent vessel used is glass manufacture, loses meaning with ultra violet lamp method for measuring.Under uviol lamp, same pigment solution is placed in test tube neutralization and is placed in beaker and measures light stability, and difference is very large certainly for the two result.For a certain amount of pigment solution, the size of ultraviolet irradiation area has a great impact certainly to measurement result, and this is in fact the thickness problem of a pigment solution.In addition, according to organic photochemistry knowledge, the photofading main cause of organic dyestuff is oxidation reaction, and its mechanism is that the irradiation of organic dyestuff light is converted into excited triplet state.The energy trasfer oxygen supply of this excited triplet state, makes oxygen become excited singlet state, and creating singlet oxygen, as oxygenant, with the effect of ground state dye molecule, through ene-type hydrogen peroxide, fades to oxygen six circle or peroxide intermediate.When organic dyestuff is in excited triplet state, measure characteristic wavelength absorbance, the absorbance of organic molecule can raise because of illumination, declines subsequently because of energy trasfer.Thus Accurate Determining monascorubin light stability is wanted should to select the initial illumination concentration of suitable pigment solution.
Because the assay method studying the monascorubin light stability that a kind of accuracy is high, repeatability is high, easy and simple to handle is necessary.
Summary of the invention
The present invention aims to provide the assay method of the monascorubin light stability that a kind of accuracy is high, repeatability is high, easy and simple to handle.
The assay method of described monascorubin light stability, comprises the following steps:
First commercially available powdery monascorubin or paste monascorubin are separated, obtain redness, salmon pink and xanthein, obtained pigment is dissolved in distilled water respectively and is made into certain density pigment solution, and be blank with distilled water, measure its initial absorbance with ultraviolet-visible pectrophotometer.Get the plate that a certain amount of pigment solution is placed in Φ 12cm, under plate being placed in 4OW uviol lamp, distance 15cm irradiates, and each 20min takes out, and surveys absorbance, pigment storage rate=(absorbance of postradiation absorbance/pre-irradiation) × 100%.
Preferably, the separation method of monascorubin of the present invention is that commercially available powdery monascorubin or paste monascorubin are first used chloroform: methyl alcohol=50:50 extracts, after the silicagel column that chloroform soaked, first use chloroform: first ferment=90:10 washes, except the free pigment that depolarization is little, use chloroform again: first ferment=50:50 washes, be separated and obtain pigment recycle silicon offset plate thin-layer chromatography, chromatography agent is chloroform: methyl alcohol: water=65:25:4.
Preferred, the mensuration wavelength of ultraviolet-visible pectrophotometer of the present invention is that redness, salmon pink and xanthein liquid are respectively 490nm, 374nm, 367nm.
Preferred further, pigment solution concentration of the present invention is 0.01%, and initial absorbance is 1.089, and the consumption of pigment solution is that thickness is 4.5mm in plate.
Salmon pink pigment containing a great deal of in usual commercially available monascorubin powder and xanthein are (according to surveying and determination, the two content is respectively 6% and about 4%), it is different that these pigments and haematochrome produce free radical ability, the number of their content can affect the weak fixed of principal ingredient haematochrome light stability in monascorubin, thus first by three kinds of pigment separateds, then the light stability of haematochrome should be measured.
Assay method of the present invention has the features such as accuracy is high, repeatability is high, easy and simple to handle.After measured, red in monascorubin, orange and yellow three kinds of pigments respectively in the light stability result of water, after ultraviolet lighting lh, haematochrome storage rate is 35%, and salmon pink is 0, and uranidin is 94%, visible yellow color element is that salmon pink is least stable to light to light most stable elements in monascorubin.
Embodiment
Embodiment one:
Commercially available powdery monascorubin or paste monascorubin are first used chloroform: methyl alcohol=50:50 extracts, after the silicagel column that chloroform soaked, first use chloroform: first ferment=90:10 washes, except the free pigment that depolarization is little, use chloroform again: first ferment=50:50 washes, separation obtains pigment recycle silicon offset plate thin-layer chromatography, and chromatography agent is chloroform: methyl alcohol: water=65:25:4.Then the redness, salmon pink and the xanthein that obtain must be separated, obtained pigment is dissolved in distilled water respectively the pigment solution being made into certain 0.01%, and be blank with distilled water, under 490nm, 374nm, 367nm, the initial absorbance of redness, salmon pink and xanthein liquid is measured respectively with ultraviolet-visible pectrophotometer.Then the plate that a certain amount of pigment solution is placed in Φ 12cm is got, the thickness of pigment solution in plate is 4.5mm, under plate being placed in 4OW uviol lamp, distance 15cm irradiates, each 20min takes out, estimate absorbance, pigment storage rate=(absorbance of postradiation absorbance/pre-irradiation) × 100%.Measurement result as shown in Table-1.
The light stability of three kinds of pigments in table-1 monascorubin
From table-1, can find out that red in monascorubin, orange and yellow three kinds of pigments are respectively in the light stability result of water, after ultraviolet lighting lh, haematochrome storage rate is 35%, salmon pink is 0, uranidin is 94%, and visible yellow color element is that salmon pink is least stable to light to light most stable elements in monascorubin.
Claims (4)
1. the assay method of monascorubin light stability, comprises the following steps:
First commercially available powdery monascorubin or paste monascorubin are separated, obtain redness, salmon pink and xanthein, obtained pigment is dissolved in distilled water respectively and is made into certain density pigment solution, and be blank with distilled water, measure its initial absorbance with ultraviolet-visible pectrophotometer;
Get the plate that a certain amount of pigment solution is placed in Φ 12cm, under plate being placed in 4OW uviol lamp, distance 15cm irradiates, and each 20min takes out, and surveys absorbance, pigment storage rate=(absorbance of postradiation absorbance/pre-irradiation) × 100%.
2. the assay method of monascorubin light stability as claimed in claim 1, it is characterized in that the separation method of described monascorubin is that commercially available powdery monascorubin or paste monascorubin are first used chloroform: methyl alcohol=50:50 extracts, after the silicagel column that chloroform soaked, first use chloroform: first ferment=90:10 washes, except the free pigment that depolarization is little, use chloroform again: first ferment=50:50 washes, separation obtains pigment recycle silicon offset plate thin-layer chromatography, and chromatography agent is chloroform: methyl alcohol: water=65:25:4.
3. the assay method of monascorubin light stability as claimed in claim 1, is characterized in that the mensuration wavelength of described ultraviolet-visible pectrophotometer is that redness, salmon pink and xanthein liquid are respectively 490nm, 374nm, 367nm.
4. the assay method of monascorubin light stability as claimed in claim 1, it is characterized in that described pigment solution concentration is 0.01%, initial absorbance is 1.089, and the consumption of pigment solution is that thickness is 4.5mm in plate.
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| CN201410565033.0A CN104330375A (en) | 2014-10-22 | 2014-10-22 | Method for measuring light stability of monascus red pigment |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108680522A (en) * | 2018-06-04 | 2018-10-19 | 福州大学 | A kind of pigment content assay method of viscosity monascorubin aqueous solution |
| CN109323982A (en) * | 2018-11-16 | 2019-02-12 | 浙江万里学院 | Many condition controls natural pigment Stability Determination device and application method |
| CN111171597A (en) * | 2020-01-15 | 2020-05-19 | 安徽农业大学 | Method for separating and purifying rubropunctatin in red yeast glutinous rice |
| CN112014319A (en) * | 2020-08-26 | 2020-12-01 | 中山大学新华学院 | Detector for dynamically detecting influence of illumination on ultraviolet absorbance of substance |
| CN113201230A (en) * | 2020-12-23 | 2021-08-03 | 天津科技大学 | Monascus pigment product and preparation method thereof |
-
2014
- 2014-10-22 CN CN201410565033.0A patent/CN104330375A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108680522A (en) * | 2018-06-04 | 2018-10-19 | 福州大学 | A kind of pigment content assay method of viscosity monascorubin aqueous solution |
| CN109323982A (en) * | 2018-11-16 | 2019-02-12 | 浙江万里学院 | Many condition controls natural pigment Stability Determination device and application method |
| CN109323982B (en) * | 2018-11-16 | 2021-01-15 | 浙江万里学院 | Multi-condition control natural pigment stability measuring device and using method |
| CN111171597A (en) * | 2020-01-15 | 2020-05-19 | 安徽农业大学 | Method for separating and purifying rubropunctatin in red yeast glutinous rice |
| CN112014319A (en) * | 2020-08-26 | 2020-12-01 | 中山大学新华学院 | Detector for dynamically detecting influence of illumination on ultraviolet absorbance of substance |
| CN112014319B (en) * | 2020-08-26 | 2021-11-23 | 中山大学新华学院 | Detector for dynamically detecting influence of illumination on ultraviolet absorbance of substance |
| CN113201230A (en) * | 2020-12-23 | 2021-08-03 | 天津科技大学 | Monascus pigment product and preparation method thereof |
| CN113201230B (en) * | 2020-12-23 | 2023-02-10 | 天津科技大学 | Monascus pigment product and preparation method thereof |
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Application publication date: 20150204 |