CN104327174A

CN104327174A - Semi-continuous extraction method of high-additional-value product in corn by-product DDGS

Info

Publication number: CN104327174A
Application number: CN201410227378.5A
Authority: CN
Inventors: 辛忠; 顾嘉辉
Original assignee: East China University of Science and Technology
Current assignee: East China University of Science and Technology
Priority date: 2014-05-27
Filing date: 2014-05-27
Publication date: 2015-02-04
Anticipated expiration: 2034-05-27
Also published as: CN104327174B

Abstract

The invention relates to a semi-continuous extraction method of high-additional-value product in corn by-product DDGS. The semi-continuous extraction method includes following steps: (1) extracting grease, alcohol-soluble proteins and corn yellow pigment from the DDGS respectively through n-hexane, a 60-95 wt% ethanol aqueous solution, and anhydrous ethanol, wherein a liquid-solid mass ratio of the extraction solvent and the raw material is 1:1-15:1 (relative to the mass of the raw material in single packed column), an extraction temperature is 25-78 DEG C, a retention time of an extraction solution in the single packed column is 5-50 min, the number of stages of an extraction device is 1-15, a disulfide bond reducing agent additive amount in extraction of the alcohol-soluble proteins is 0-0.1 wt% (relative to the mass of the extraction solution, and a pH range of the extraction solution is 1-6; and (2) removing the solvent in an evaporation manner from the extraction solution in each steps, and properly purifying the mixture to obtain the high-additional-value product including the grease, the alcohol-soluble proteins and the corn yellow pigment. In the method, the semi-continuous extraction method is employed for processing the DDGS. Compared with a batch extraction method, the method can greatly reduce a use amount of the solvent, can increase the concentration of the extraction solution and meanwhile can greatly increase a treatment amount of the raw material. The semi-continuous extraction method is an effective method being suitable for large-scale industrialized extraction.

Description

The extracting method of high value added product in a kind of semicontinuous corn by-product DDGS

Technical field

The present invention relates to a kind of field of Corn Fuel Ethanol by product DDGS high value added utilization, be specifically related to the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS.

Background technology

The fuel ethanol industrial of China is greatly developed by the beginning of 21 century, country " 15 " and Eleventh Five-Year Plan plan support under, it is built that what comprise Jilin fuel ethanol company take corn as 4 large fuel ethanol production companies of raw material, the annual production of 1,300,000 tons is just reached in 2006, China has become third place in the world mcroorganism fuel ethanol production state and application state after Brazil, the U.S. in the world, expect the year two thousand twenty, the alcohol fuel output of China will reach 1,000 ten thousand tons, current Chinese alcohol fuel main raw material or W-Gum.

Be below the improvement wet method fuel ethanol production technique based on W-Gum that domestic each main fuel ethanol company generally adopts, see Figure of description 1.

Corn is through clearing up, soaking, go the techniques such as embryo, fine grinding, liquefaction, saccharification, fermentation, rectifying to obtain target product alcohol fuel, and remaining substrate is through the dry animal-feed DDGS(Distillers Dried Grains with Solubles becoming high protein, maize alcohol stillage albumen and solvend).

This technique eliminates the step that starch removes, effectively improve the production efficiency of alcohol fuel, but the less expensive of its bulk product ethanol and DDGS, it is low that each alcohol fuel company is still in profit, need the condition of the long-term subsidy of country, therefore the high value added product of Corn Fuel Ethanol by product DDGS is extracted, develop to solve very well and improve fuel ethanol industrial profit, widen the problem of downstream industry chain.

Containing a considerable number of grease in DDGS feed, corn yellow OB, the compositions such as zein, ethanol is prepared because starch has wherein been fermented, the content of the high value added product in DDGS feed will be much higher than corn, wherein grease due to acid number high, inedibility, but its lipid acid composition is more or less the same with refined corn soil, can be used for preparing biofuel, corn yellow OB is a Carotenoids, and its main component xenthophylls and zeaxanthin are the main components forming human lens macula lutea, are excellent antioxidant, it contains a large amount of conjugated double bonds, by quenching singlet oxygen, the Anti-oxidation behavior such as scavenging free radicals carry out protecting group body tissue cell, and it is treating cataract to have research, vision protection, anti-cancer and cancer-preventing aspect has special effect, can purify as eyeshield healthcare products, and zein is a kind of hydrophobic natural degradable macromole, it has unique film forming ability, in alcohol solution, become random ball of string structure, namely can be formed transparent after solvent evaporation, glossiness film, the film obtained has protection against the tide, oxygen barrier, uvioresistant, protect fragrant, oil resistance, the characteristics such as anti-electrostatic, can as tablet coating on medical, medicine sustained release dosage (slow releasing capsule), in the food industry for chocolate, its glossiness of coated increase of healthcare products, mouthfeel, extend its quality guaranteed period, can also as chewing gum sweet taste sustained release dosage, can also as degradable food product pack after some physical and chemical modified, Perserving materials.

And now bibliographical information to corn, maize yellow-powder albumen, the extraction of the extracting method of corn yellow OB and zein mainly batch tank in maize alcohol stillage protein fodder, the shortcoming of this method is that solvent load is large, in extracting solution, target product content is low, solvent observable index is boiled off higher after extraction, and step extracting mode is also very low to the processing efficiency of material, be only suitable for laboratory or small serial production, as the extraction process for DDGS, the DDGS feed throughput of hundreds of thousands of ton easily in fuel ethanol industry cannot be met at all, the alcohol fuel company of China seldom considers how to go to utilize it, the DDGS raw material of a large amount of preciousness is abandoned by as cheap feed or conventional movement, this is the significant wastage to corn resources, therefore exploitation one can reduce extracting solution consumption, increase target product concentration, the DDGS high value added product extraction process improving feed throughput has great application prospect and economic benefit.

Summary of the invention

The object of the invention is to overcome the deficiencies in the prior art, the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS is provided.

The extractive technique route that the present invention adopts is shown in Figure of description 2.

This technological line mainly comprises grease extraction, prolamine extracts, prolamine decolours three parts, obtain three high added value target products respectively: grease, prolamine, corn yellow OB, wherein acid value of oil and fat is very high, can as biodiesel raw material, prolamine can be developed as functional high molecule material, and corn yellow OB can be purified as eyeshield healthcare products.

The object of the invention is to be achieved through the following technical solutions.

The invention provides the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS, Figure of description 3 is shown in by the extraction element schematic diagram that it adopts.

This device is that a kind of plural serial stage fills the semicontinuous extraction equipment of pillar, its operation logic is: raw material DDGS is filled in several identical column extractor as stationary phase, suppose that in DDGS, a kind of target product needs fixing liquid-solid ratio Extraction solvent extraction could extract complete n time, so this semicontinuous device progression is n+1 level, and before formal driving, 1# post ~ n-1# post first first can extract n-1 ~ 1 time by the extraction agent of this fixing liquid-solid ratio respectively, this pre-treatment can make each post form certain concentration gradient, during formal driving, again by the extracting solution of this fixing liquid-solid ratio again from 1# ~ n# post Continuous Countercurrent Extraction one time, complete first and extract circulation, then 1# post can carry out slag dumping after being extracted n time, then former n+1 level for subsequent use is as last 1 grade (namely n-th grade) of second circulation, the progression of 2# ~ n# post of first circulation is decremented to 1 ~ n-1 level successively second circulation, in the semi-continuous apparatus of this n+1 level like this, always there is n level packed column in operation, 1 grade as spare level with changing raw material, complete each extraction circulation successively, the extracting solution passed through can be made like this to make full use of the concentration gradient of each post, not only decrease total consumption of solvent, decrease energy consumption, and improve the concentration of target product in extracting solution, also improve the treatment capacity of raw material simultaneously.

The invention provides the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS, comprise.

(1) grease extraction step.

The liquid-solid mass ratio (compared to single packed column DDGS quality) of Extraction solvent normal hexane and DDGS is 1:1 ~ 15:1, Extracting temperature is 25 ~ 68.7 DEG C, extracting solution is all 5min ~ 40min by single packed column required time, progression needed for semicontinuous extraction process is 1 ~ 15 grade, the extracting solution obtained, only need boil off normal hexane agent can obtain grease.

Described maize alcohol stillage albumen is provided by the alcohol fuel company using corn as raw material, the lurid product of preferred color and luster.

Described grease Extraction solvent is nontoxic food grade normal hexane.

Described normal hexane and the liquid-solid mass ratio of DDGS (compared to single packed column DDGS quality) are 1:1 ~ 3:1, Extracting temperature 40 ~ 68.7 DEG C, extracting solution is all 5min ~ 15min by single packed column required time, and progression needed for semicontinuous extraction process is 4 ~ 10 grades.

(2) prolamine extraction step.

Continue as raw material using the remaining residue of step (1), the aqueous ethanolic solution of Extraction solvent 60 ~ 95wt% and the liquid-solid mass ratio (compared to single packed column DDGS quality) of initial DDGS are 1:1 ~ 15:1, Extracting temperature is 25 ~ 78 DEG C, protein disulfide reductive agent addition is that 0 ~ 0.1%(is relative to extracting solution quality), extracting solution pH value scope is 1 ~ 6, extracting solution is all 10min ~ 50min by single packed column required time, progression needed for semicontinuous extraction process is 1 ~ 15 grade, the extracting solution obtained, first boil off ethanol, surplus solution is through high speed centrifugation, the precipitation drying obtained is the prolamine being rich in corn yellow OB, and clear liquor is the aqueous solution of soluble sugar.

Described prolamine Extraction solvent is nontoxic aqueous ethanolic solution, and its concentration is preferably 70 ~ 80wt%.

Described aqueous ethanolic solution and the liquid-solid mass ratio of DDGS (compared to single packed column DDGS quality) are 1:1 ~ 3:1, Extracting temperature is 50 ~ 78 DEG C, pH value scope is 2 ~ 5, extracting solution is all 20min ~ 40min by single packed column required time, and progression needed for semicontinuous extraction process is 4 ~ 10 grades.

Described protein disulfide reductive agent is asepsis environment-protecting, be soluble in alcohol solution and can as the L-cysteine hydrochloride of feed nutrition supplement, and its addition is that 0.01wt% ~ 0.05wt%(is relative to extracting solution quality).

(3) prolamine decolorization process.

By the prolamine obtained in step (2) through pulverizing 20 mesh sieves, the liquid-solid mass ratio of Extraction solvent dehydrated alcohol and prolamine is 1:1 ~ 15:1, Extracting temperature 25 ~ 78 DEG C, extracting solution is all 10min ~ 50min by single packed column required time, and needed for semicontinuous extraction process, progression is 1 ~ 15 grade, obtains extracting solution, through high speed centrifugation, lower sediment drying is the prolamine after removing look, and clear liquor, through boiling off ethanol, namely obtains the corn yellow OB of reddish yellow.

Described prolamine decolouring solvent is nontoxic dehydrated alcohol.

The liquid-solid mass ratio of described dehydrated alcohol and prolamine liquid is 1:1 ~ 3:1, Extracting temperature 50 ~ 78 DEG C, and extracting solution is all 10min ~ 30min by single packed column required time, and progression needed for semicontinuous extraction process is 4 ~ 10 grades.

Compared with prior art, positively effect of the present invention is.

(1) present invention employs semicontinuous device, high value added product by DDGS: grease, prolamine, corn yellow OB extract successively, especially for the extraction of prolamine and corn yellow OB, the Extraction solvent consumption overcoming the existence of traditional batch extraction process is large, in extracting solution, target product concentration is low, the shortcoming that material treatment capacity is little.

(2) this patent adopts innoxious solvent as extraction agent, it is a kind of extraction process of green non-pollution, particularly in prolamine extracts, adopt asepsis environment-protecting, be soluble in alcohol solution and can as the L-cysteine hydrochloride of feed nutrition supplement as disulfide bond reducing agent, it can with alcohol solution rapid diffusion in filling raw material, make the intermolecular disulfide bonds of larger prolamine, make it more be soluble in extracting solution, strengthen prolamine yield.

(3) the present invention is the commercial viability that the Corn Fuel Ethanol industry be in trouble provides based on extracting high value added product in large cheap by product DDGS.

Accompanying drawing explanation

Fig. 1 is the improvement wet method fuel ethanol production technique based on W-Gum that domestic each main fuel ethanol company generally adopts.

Fig. 2 is the extractive technique route that the present invention adopts.

Fig. 3 is the extraction element schematic diagram that the present invention adopts.

Embodiment

Describe the present invention below in conjunction with embodiment, but the following example should not regard limitation of the scope of the invention as.

The present embodiment maize alcohol stillage albumen used is provided by Jilin fuel ethanol company limited, and wherein fat, protein, maize yellow cellulose content are respectively 5.9wt%, 29.8wt%, 89.8 μ g/g.

Embodiment 1: semicontinuous grease extraction step.

12gDDGS is fixed in the packed column of tool hydro-thermal chuck, 5 grades of semicontinuous devices are set, the normal hexane getting 36g carries out the preextraction (note: each extraction all changes fresh solvent) of 3 ~ 1 times respectively respectively to 1# ~ 3# post, form certain grease concentration gradient, then normally drive, get the 36g normal hexane post Continuous Countercurrent Extraction from the bottom up from 1# to 4# successively, the extraction conditions of each post is 45 DEG C, residence time 10min, the extracting solution collected from 4# post is final extracting solution, boil off normal hexane, obtain target product grease, first loop ends, 1# post is owing to being extracted 4 times, extract complete, transfer stand-by column to renewing material, 5# post is then as the 4th grade of the second circulation extraction, and 2# ~ 4# post of former first circulation is decremented to 1st ~ 3 grades of the second circulation respectively, these 4 grades of packed columns are passed through successively again with 36g normal hexane, complete second circulation, the extraction work of 8 circulations is completed successively according to this method, the extracting solution obtained obtains grease after removing normal hexane, find that in the extracting solution that 8 circulations obtain, fat content change is little, average quality content is 1.8wt%, grease yield reaches 94.5wt%, in grease, maize yellow cellulose content is 525.4 μ g/g(yields is 41.4wt%).

Embodiment 2: semicontinuous prolamine extraction step.

Get the residue that 12g step (1) unloads, be fixed in the packed column of tool hydro-thermal chuck, 4 grades of semicontinuous devices are set, get the 70wt% aqueous ethanolic solution (pH value 4 of 36g, L-cysteine hydrochloride content 0.03wt%) respectively 1# ~ 2# post is carried out respectively to the preextraction (note: each extraction all change fresh solvent) of 2 ~ 1 times, form certain prolamine concentration gradient, then normally drive, get the 70wt% aqueous ethanolic solution (pH value 4 of 36g, L-cysteine hydrochloride content 0.03wt%) the post Continuous Countercurrent Extraction from the bottom up from 1# to 3# successively, the extraction conditions of each post is 78 DEG C, residence time 40min, the extracting solution collected from 3# post is final extracting solution, boil off ethanol, after solution high speed centrifugation, the precipitation obtained is target product prolamine, first loop ends, 1# post is owing to being extracted 3 times, extract complete, transfer stand-by column to renewing material, 4# post is then as the 3rd level that the second circulation is extracted, and 2# ~ 3# post of former first circulation is decremented to 1st ~ 2 grades of the second circulation respectively, again with the 70wt% aqueous ethanolic solution (pH value 4 of 36g, L-cysteine hydrochloride content 0.03wt%) successively by these 3 grades of packed columns, complete second circulation, the extraction work of 8 circulations is completed successively according to this method, find that in the extracting solution that 8 circulations obtain, prolamine average quality content is 2.24wt%, prolamine yield reaches 7.3wt%, (accounting for total DDGS quality), in prolamine, maize yellow cellulose content is 479.8 μ g/g(yields is 37.9wt%).

Embodiment 3: prolamine decolorization process continuously.

Get the dried prolamine that 12g step (2) obtains, be fixed in the packed column of tool hydro-thermal chuck, 4 grades of semicontinuous devices are set, the dehydrated alcohol getting 36g carries out the preextraction (note: each extraction all changes fresh solvent) of 2 ~ 1 times respectively respectively to 1# ~ 2# post, form certain pigment concentration gradient, then normally drive, get the dehydrated alcohol post Continuous Countercurrent Extraction from the bottom up from 1# to 3# successively of 36g, the extraction conditions of each post is 55 DEG C, residence time 20min, the extracting solution collected from 3# post is final extracting solution, boil off ethanol, namely corn yellow OB crude extract is obtained, and residual residue is the prolamine after discoloring after drying in packed column, first loop ends, 1# post is owing to being extracted 3 times, extract complete, transfer stand-by column to renewing material, 4# post is then as the 3rd level that the second circulation is extracted, and 2# ~ 3# post of former first circulation is decremented to 1st ~ 2 grades of the second circulation respectively, these 3 grades of packed columns are passed through successively again with 36g dehydrated alcohol, complete second circulation, the extraction work of 8 circulations is completed successively according to this method, to find in the residue prolamine that 8 circulations obtain that corn yellow OB average content is that to remain yields be 11.7wt% to 180.8 μ g/g().

Comparative example 1: interval grease extraction step.

Get 36g normal hexane to add and fill in the there-necked flask of 12gDDGS, heated and stirred backflow 10min in the water bath with thermostatic control of 45 DEG C of degree, after extracting solution removes residue after filtration, filtrate removes normal hexane by rotary evaporation, namely grease is obtained, in extracting solution, oil quality mark is 1.3wt%, and grease yield reaches 67.6wt%, and in grease, maize yellow cellulose content is 872.8 μ g/g(yields is 38.7wt%).

Compared with extracting with grease semicontinuous in embodiment, under interval grease is extracted in identical extraction conditions, in the extracting solution obtained, oil quality mark reduces 27.8wt%(relative to half-continuous process), grease yield reduces 28.5wt%(relative to half-continuous process), and one batch feed throughput is 1/4th of half-continuous process.

Comparative example 2: interval prolamine extraction step.

The 70wt% aqueous ethanolic solution getting 36g adds in the there-necked flask filling residue in 12g step (1), heated and stirred backflow 40min in the water bath with thermostatic control of 78 DEG C of degree, reductive agent L-cysteine hydrochloride addition is 10.8mg, extracting solution pH value is 4, after extracting solution removes residue after filtration, filtrate removes ethanol by rotary evaporation, the turbid solution obtained is through high speed centrifugation, lower sediment is the prolamine being rich in corn yellow OB, obtaining prolamine average quality content in extracting solution is 1.78wt%, prolamine yield reaches 5.3wt%, (accounting for total DDGS quality), in prolamine, maize yellow cellulose content is 562.7 μ g/g(yields is 33.4wt%).

Compared with extracting with prolamine semicontinuous in embodiment, under interval prolamine is extracted in identical extraction conditions, in the extracting solution obtained, prolamine massfraction reduces 20.5wt%(relative to half-continuous process), prolamine yield reduces 27.4wt%(relative to half-continuous process), and one batch feed throughput is 1/3rd of half-continuous process.

Comparative example 3: interval prolamine decolorization process.

The dehydrated alcohol getting 36g adds in the there-necked flask filling the prolamine obtained in 12g step (2), heated and stirred backflow 20min in the water bath with thermostatic control of 55 DEG C of degree, in the molten albumen that extracting solution obtains through high speed centrifugation, maize yellow cellulose content is that to remain yields be 16wt% to 268.1 μ g/g().

Compared with decolouring with prolamine semicontinuous in embodiment, the decolouring of interval prolamine is under identical extraction conditions, the prolamine pigment content obtained adds 48.3wt%(relative to semicontinuous extraction process), and one batch feed throughput is 1/3rd of half-continuous process.

More than show and describe ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these change and this enters all to fall in the claimed scope of the invention.Application claims protection domain has appending claims and equivalent thereof to define.

Claims

1. the extracting method of high value added product in semicontinuous corn by-product DDGS, comprising:

Have employed a kind of plural serial stage and fill the semicontinuous extraction element of pillar, the grease in DDGS is extracted respectively with solvent-extraction process, prolamine, corn yellow OB, wherein the liquid-solid mass ratio of Extraction solvent and raw material is that 1:1 ~ 15:1(is relative to single packed column raw materials quality), Extracting temperature 25 ~ 78 DEG C, extracting solution is 5min ~ 50min in the single packed column residence time, extraction element progression is at 1 ~ 15 grade, wherein in prolamine extraction, disulfide bond reducing agent addition is that 0 ~ 0.1wt%(is relative to extracting solution quality), extracting solution pH value is 1 ~ 6, and the extracting solution that each step obtains is removing through solvent steaming, just high value added product grease can be obtained after suitably refining, prolamine and corn yellow OB.

2. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 1, it is characterized in that, selected device is that a kind of plural serial stage fills the semicontinuous extraction element of pillar, its operation logic is: suppose that in DDGS, a kind of target product needs fixing liquid-solid ratio Extraction solvent extraction could extract complete n time, so this semicontinuous device progression is n+1 level, and before formal driving, 1# post ~ n-1# post first first can extract n-1 ~ 1 time by the extraction agent of this fixing liquid-solid ratio respectively, this pre-treatment can make each post form certain concentration gradient, during formal driving, again by the extracting solution of this fixing liquid-solid ratio again from 1# ~ n# post Continuous Countercurrent Extraction one time, complete first and extract circulation, 1# post can carry out slag dumping after being extracted n time, then former n+1 level for subsequent use is as last 1 grade (namely n-th grade) of second circulation, the progression of 2# ~ n# post of first circulation is decremented to 1 ~ n-1 level successively second circulation, in the semi-continuous apparatus of this n+1 level like this, always there is n level packed column in operation, 1 grade as spare level with changing raw material, complete each extraction circulation successively.

3. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 2, is characterized in that, in this semi-continuous apparatus, extracting solution is inject continuously, and to extract material be that interval is changed, and is presented as a kind of half-continuous process on the whole.

4. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 1, it is characterized in that, the color of selected corn DDGS requires, for more shallow yellow, strictly to give up the scarlet DDGS that excessive high temperature drying causes corn yellow OB and prolamine sex change.

5. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 1, it is characterized in that, described grease extraction agent, prolamine extraction agent, corn yellow OB extraction agent be respectively nontoxic food grade normal hexane, 60 ~ 95% aqueous ethanolic solution, dehydrated alcohol.

6. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 1, it is characterized in that, described grease extraction step (1): the liquid-solid mass ratio (compared to single packed column DDGS quality) of Extraction solvent and DDGS is 1:1 ~ 15:1, Extracting temperature is 25 ~ 68.7 DEG C, extracting solution is all 5min ~ 40min by single packed column required time, progression needed for semicontinuous extraction process is 1 ~ 15 grade, the extracting solution obtained, only need boil off normal hexane agent can obtain grease.

7. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 6, it is characterized in that, grease extraction step (1): described Extraction solvent is nontoxic food grade normal hexane, the liquid-solid mass ratio (compared to single packed column DDGS quality) of described normal hexane and DDGS is 1:1 ~ 3:1, described Extracting temperature is 40 ~ 68.7 DEG C, described extracting solution is all 5min ~ 15min by single packed column required time, and needed for described semicontinuous extraction process, progression is 4 ~ 10 grades.

8. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 1, it is characterized in that, described prolamine extraction step (2): continue as raw material using the remaining residue of step (1), the aqueous ethanolic solution of Extraction solvent 60 ~ 95wt% and the liquid-solid mass ratio (compared to single packed column raw materials quality) of raw material are 1:1 ~ 15:1, Extracting temperature is 25 ~ 78 DEG C, protein disulfide reductive agent addition is that 0 ~ 0.1wt%(is relative to extracting solution quality), extracting solution pH value is 1 ~ 6, extracting solution is all 10min ~ 50min by single packed column required time, progression needed for semicontinuous extraction process is 1 ~ 15 grade, the extracting solution obtained, first boil off ethanol, remaining aqueous solution is through high speed centrifugation, the precipitation drying obtained is the prolamine being rich in corn yellow OB.

9. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 8, it is characterized in that, prolamine extraction step (2): described Extraction solvent is the aqueous ethanolic solution of 70 ~ 80wt%, the liquid-solid mass ratio (compared to single packed column DDGS quality) of described aqueous ethanolic solution and raw material is 1:1 ~ 3:1, described Extracting temperature is 50 ~ 78 DEG C, described extracting solution pH value is acid range 2 ~ 5, described extracting solution is all 20min ~ 40min by single packed column required time, needed for described semicontinuous extraction process, progression is 4 ~ 10 grades.

10. the extracting method of high value added product in a kind of semicontinuous corn by-product DDGS according to claim 8, it is characterized in that, prolamine extraction step (2): described protein disulfide reductive agent is asepsis environment-protecting, be soluble in alcohol solution and can as the L-cysteine hydrochloride of nutritious supplementary, and described protein disulfide reductive agent addition is that 0.01wt% ~ 0.05wt%(is relative to extracting solution quality).

The extracting method of high value added product in 11. a kind of semicontinuous corn by-product DDGS according to claim 1, it is characterized in that, described prolamine decolorization process (3): after the prolamine drying obtained in step (2) is pulverized, the liquid-solid mass ratio of Extraction solvent dehydrated alcohol and prolamine is 1:1 ~ 15:1, Extracting temperature 25 ~ 78 DEG C, extracting solution is all 10min ~ 50min by single packed column required time, progression needed for semicontinuous extraction process is 1 ~ 15 grade, obtain extracting solution, through high speed centrifugation, lower sediment drying is the prolamine after removing look, clear liquor is through boiling off ethanol, be the corn yellow OB of reddish yellow.

The extracting method of high value added product in 12. a kind of semicontinuous corn by-product DDGS according to claim 11, it is characterized in that, prolamine decolorization process (3): described prolamine granularity was 20 mesh sieves, described prolamine discoloring agent is nontoxic dehydrated alcohol, the liquid-solid mass ratio of described dehydrated alcohol and prolamine liquid is 1:1 ~ 3:1, Extracting temperature 50 ~ 78 DEG C, extracting solution is all 10min ~ 30min by single packed column required time, and progression needed for semicontinuous extraction process is 4 ~ 10 grades.