CN104316611A - Method for measuring content of sucralose - Google Patents
Method for measuring content of sucralose Download PDFInfo
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- CN104316611A CN104316611A CN201410573802.1A CN201410573802A CN104316611A CN 104316611 A CN104316611 A CN 104316611A CN 201410573802 A CN201410573802 A CN 201410573802A CN 104316611 A CN104316611 A CN 104316611A
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Abstract
The invention relates to a method for measuring the content of sucralose, belonging to the field of food additives and detection methods of food additives. The method for measuring the content of sucralose comprises the following steps: 1, preparation of a standard solution, namely accurately weighing about 25mg of sucralose standard, putting in a 25mL volumetric flask, dissolving with a mobile phase to reach a constant volume, and filtering the obtained solution by a 0.45mu m filter membrane to obtain a filtrate for later use; 2, preparation of a sample, namely accurately weighing about 25mg of a test sample, putting in a 25mL volumetric flask, dissolving with a mobile phase to reach a constant volume, and filtering the obtained solution by a 0.45mu m filter membrane to obtain a filtrate for later use; and 3, analysis process: analyzing the standard solution and the sample solution under a certain chromatographic condition, injecting 20 microliters of the sample twice, calculating the peak area of the principal peak and calculating the percentage content of sucralose on the basis of the peak area Au of the sample solution and the peak area As of the standard solution according to the following formula: the percentage content (%) of sucralose= (the peak area of the sample solution Au* the weight of the standard)/the peak area of the standard solution As* the weight of the test sample)*100. High performance liquid chromatography is adopted in the method for measuring the content of sucralose; the method is simple, rapid, high in sensitivity and good in reproducibility and can provide accurate and reliable results; therefore, the method is applicable to monitoring and detection in a production process and by manufacturers using the method.
Description
Technical field
The present invention relates to a kind of assay method of Sucralose content, belong to food additives and detection method field thereof.
Background technology
Sucralose, white or yellow-white, odorlessness, pleasantly sweet crystalline powder, in water-soluble, methyl alcohol, ethanol, can be slightly soluble in acetic acid.Purposes in food: Sucralose is a kind of apotrophic sweetener, metabolism is not participated in human body, not carious tooth, mouthfeel is similar to sucrose, in water, solubleness is high, industrial processes is stablized and with other compositions in food without interaction, is become the high sugariness that application is very wide, without the sweetener of calorific value.June nineteen ninety, FAO/WHO organizes the food additives joint specialist council to have approved the permanent ADI value of Sucralose for 15mg/kg, 16th time national food additives technical committee for standardization (TCST) passes through discussion, the maximum use amount of Sucralose in the food such as beverage, pickles, composite flavor, assembled alcoholic drinks, cake, biscuit, bread, ice lolly, ice cream is allowed to be 0.25g/kg, the maximum use amount of chewing gum, preserved fruit is 1.5g/kg, the maximum use amount of mensal sweetening agent is 0.05g/ bag. sheet, canned fruit is 0.25g/kg.Sucralose is widely applied in food.But the detection of food additives Sucralose content is without national standard or industry standard methods at present.
Therefore, a kind of method that is easy, quick, Accurate Determining Sucralose content is set up necessary.
Summary of the invention
The present invention aims to provide a kind of method of easy, quick, Accurate Determining Sucralose content.
The assay method of Sucralose content of the present invention, comprises the following steps:
(1) standard solution preparation:
The Sucralose standard items accurately taking about 25mg are in 25mL volumetric flask, and dissolve and constant volume with mobile phase, gained solution 0.45 μm of membrane filtration, filtrate is for subsequent use.
(2) preparation of samples
The trial target accurately taking about 25mg is in 25mL volumetric flask, and dissolve and constant volume with mobile phase, gained solution 0.45 μm of membrane filtration, filtrate is for subsequent use.
(3) analytic process:
Analytical standard solution and sample solution under certain chromatographic condition, sample introduction 20 μ L twice, calculates main peak peak area, by the peak area As of sample solution peak area Au and standard solution, the percentage composition according to following formulae discovery Sucralose:
The weight of the peak area * standard items of the percentage composition %=(sample solution of Sucralose)/(weight of the peak area * sample of standard solution) * 100.
Preferably, high performance liquid chromatograph of the present invention adopts HPLC1090M high performance liquid chromatograph, and chromatographic condition is: pillar is 8mm*10cm, 5 μm of RadPakC18 posts; Column temperature is room temperature; Flow rate of mobile phase is 1.0ml/min; Detecting device is HP1047A refraction detector.
Preferred, mobile phase of the present invention is the distilled water that trifluoroacetic acid aqueous solution+850mL that 150mL 0.45 μm of membrane filtration is crossed crosses with 0.45 μm of membrane filtration, mixing, thoroughly degassed mixed liquor.
Assay method of the present invention adopts high performance liquid chromatography, and this method has simple and quick, highly sensitive, favorable reproducibility, the result feature such as accurately and reliably.Therefore, assay method of the present invention is applicable to monitoring and the detection of production run and application producer thereof.
Embodiment
Embodiment one:
(1) standard solution preparation:
The Sucralose standard items accurately taking about 25mg are in 25mL volumetric flask, and dissolve and constant volume with mobile phase, gained solution 0.45 μm of membrane filtration, filtrate is for subsequent use.Mobile phase is the distilled water that trifluoroacetic acid aqueous solution+850mL that 150mL 0.45 μm of membrane filtration is crossed crosses with 0.45 μm of membrane filtration, mixing, thoroughly degassed mixed liquor.
(2) preparation of samples
The trial target accurately taking about 25mg is in 25mL volumetric flask, and dissolve and constant volume with above-mentioned mobile phase, gained solution 0.45 μm of membrane filtration, filtrate is for subsequent use.
(3) analytic process
Adopt HPLC1090M high performance liquid chromatograph, chromatographic condition is: pillar is 8mm*10cm, 5 μm of RadPakC18 posts; Column temperature is room temperature; Flow rate of mobile phase is 1.0ml/min; Detecting device is HP1047A refraction detector, analytical standard solution and sample solution, sample introduction 20 μ L twice, calculates main peak peak area, by the peak area As of sample solution peak area Au and standard solution, and the percentage composition according to following formulae discovery Sucralose:
The weight of the peak area * standard items of the percentage composition %=(sample solution of Sucralose)/(weight of the peak area * sample of standard solution) * 100.
Embodiment two: reappearance is tested
(2) assay method of the present invention carries out 5 parallel sample mensuration, Sucralose content is 99.30%, 99.36%, 99.28%, 99.53%, 99.49%, average out to 99.39%, result statistics shows, the repeatability of measurement result is better, standard deviation is 0.0884, and the coefficient of variation is 0.11%, this show the reappearance of this method and precision all better.
Claims (3)
1. the assay method of Sucralose content, comprises the following steps:
(1) standard solution preparation:
The Sucralose standard items accurately taking about 25mg are in 25mL volumetric flask, and dissolve and constant volume with mobile phase, gained solution 0.45 μm of membrane filtration, filtrate is for subsequent use;
(2) preparation of samples:
The trial target accurately taking about 25mg is in 25mL volumetric flask, and dissolve and constant volume with mobile phase, gained solution 0.45 μm of membrane filtration, filtrate is for subsequent use;
(3) analytic process:
Analytical standard solution and sample solution under certain chromatographic condition, sample introduction 20 μ L twice, calculates main peak peak area, by the peak area As of sample solution peak area Au and standard solution, the percentage composition according to following formulae discovery Sucralose:
The weight of the peak area * standard items of the percentage composition %=(sample solution of Sucralose)/(weight of the peak area * sample of standard solution) * 100.
2. the assay method of Sucralose content as claimed in claim 1, it is characterized in that described high performance liquid chromatograph adopts HPLC1090M high performance liquid chromatograph, chromatographic condition is: pillar is 8mm*10cm, 5 μm of RadPakC18 posts; Column temperature is room temperature; Flow rate of mobile phase is 1.0ml/min; Detecting device is HP1047A refraction detector.
3. the assay method of Sucralose content as claimed in claim 1 or 2, it is characterized in that described mobile phase is the distilled water that trifluoroacetic acid aqueous solution+850mL that 150mL 0.45 μm of membrane filtration is crossed crosses with 0.45 μm of membrane filtration, mixing, the mixed liquor after thoroughly degassed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573802.1A CN104316611A (en) | 2014-10-24 | 2014-10-24 | Method for measuring content of sucralose |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573802.1A CN104316611A (en) | 2014-10-24 | 2014-10-24 | Method for measuring content of sucralose |
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| CN104316611A true CN104316611A (en) | 2015-01-28 |
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| CN201410573802.1A Pending CN104316611A (en) | 2014-10-24 | 2014-10-24 | Method for measuring content of sucralose |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445118A (en) * | 2018-03-20 | 2018-08-24 | 广东产品质量监督检验研究院（国家质量技术监督局广州电气安全检验所、广东省试验认证研究院、华安实验室） | The rapid extraction and detection method of Sucralose in food |
| CN112748192A (en) * | 2020-11-27 | 2021-05-04 | 浙江工业大学 | Method for detecting trace sucralose in water environment by solid phase extraction-gas chromatography-mass spectrometry |
-
2014
- 2014-10-24 CN CN201410573802.1A patent/CN104316611A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445118A (en) * | 2018-03-20 | 2018-08-24 | 广东产品质量监督检验研究院（国家质量技术监督局广州电气安全检验所、广东省试验认证研究院、华安实验室） | The rapid extraction and detection method of Sucralose in food |
| CN112748192A (en) * | 2020-11-27 | 2021-05-04 | 浙江工业大学 | Method for detecting trace sucralose in water environment by solid phase extraction-gas chromatography-mass spectrometry |
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Application publication date: 20150128 |