CN104312965A - Decoloration bacterium and application thereof - Google Patents
Decoloration bacterium and application thereof Download PDFInfo
- Publication number
- CN104312965A CN104312965A CN201410597427.4A CN201410597427A CN104312965A CN 104312965 A CN104312965 A CN 104312965A CN 201410597427 A CN201410597427 A CN 201410597427A CN 104312965 A CN104312965 A CN 104312965A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- decolouring
- application
- acinetobacter
- decoloration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
- C02F2103/28—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof from the paper or cellulose industry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a decoloration bacterium and an application thereof. The decoloration bacterium is Acinetobacter sp. YH-02, with a collection number of CGMCC No. 9413, and the strain of the Acinetobacter sp. can be used to realize decoloration and COD removal of papermaking wastewater. According to the application, the strain is inoculated to an LB culture medium, is subjected to oscillation culture at 30 DEG C till the bacterium concentration is O.D600 which is approximately equal to 2.0, is inoculated to the papermaking wastewater with the inoculum size of 5-8%, and is cultured at 30-35 DEG C for 3 to 5 days, wherein the dissolved oxygen is maintained above 3.0mg/L; a supernatant is obtained through centrifuging and is determined in color scale and COD, the results show that the decoloration effect is obvious, and meanwhile, the strain has a certain COD removal capability; further, compared with physical and chemical decoloration methods and a traditional decoloration microorganism, the strain is advantageous and has a very good application values in the papermaking wastewater treatment process.
Description
Technical field
The present invention relates to biological technical field, especially a kind of decolouring bacterium and application.
Background technology
Paper industry uses timber, straw, reed, rag etc. to be raw material, isolates Mierocrystalline cellulose, make paper pulp through High Temperature High Pressure boiling.In process of production, finally the waste part of discharging in raw material is called black liquid.Containing xylogen, Mierocrystalline cellulose, VFA etc. in black liquor, frowziness, contaminative is very strong, contained pollutent has accounted for more than 90% of industrial pollution caused by paper manufacturing total emission volumn, because black liquor alkalescence is large, color is dark, stink is heavy, foam is many, and consume oxygen in water in a large number, seriously polluted source; And the chlorine-contained wastewater that middle section water environmental pollution the most seriously produces in bleaching process, such as Chlorinated Bleaching waste water, hypo(chlorite)bleaching waste water etc., also containing toxic extremely strong carcinogenic substance Dioxins, what have also contains some heavy metal elements as lead etc., after this kind of material enters water body with sewage, hydrobiological existence can be endangered, bring harm to environment and human health.
Therefore, the reuse of paper waste and zero release become development trend.But chromaticity of paper-making waste water is higher, often become brown color even black.Visible, colourity becomes the factor that affect paper waste reuse more serious than COD.
At present, the method mainly coagulation air-float of chromaticity of paper-making waste water is removed.Coagulation points in waste water to add coagulating agent; eliminate or reduce the intergranular mutual repulsive force of water-borne glue body; make water-borne glue body particle be easy to mutually collision and agglomeration overlaps and forms larger particles or flocs unit, and then separate from water, with the method for purifying liquid waste.Conventional coagulating agent comprises: 1. inorganic salts, has aluminium salt (Tai-Ace S 150, potassium aluminium sulfate, potassium aluminate etc.), molysite (iron trichloride, ferrous sulfate, ferric sulfate etc.) and magnesiumcarbonate etc.; 2. polymer substance, has polymerize aluminum chloride, polyacrylamide etc.Air supporting refers to the micro-bubble of height of formation dispersion in water, adhere to solid or the liquid particle of hydrophobic group in waste water, form water-gas-particle three-phase mixed system, after particle adhesion bubble, form apparent density be less than the flco of water and float to the water surface, form scum layer to be struck off, thus realize the process of solid-liquid or Liquid liquid Separation.
Coagulation air-float, as physico-chemical process, exists that building cost is high, operation and maintenance cost is high, sludge output causes the shortcomings such as secondary pollution greatly, easily.Therefore, biological decolouring method becomes the investigation and application focus in this field.Biological decolouring comprises the absorption of the material causing colourity and degraded two processes.At present, the major microorganisms of biological decolouring is white-rot fungi class, algae and photosynthetic bacterium, and these microorganisms often cannot coordinate with common aerobic heterotrophism Sewage treatment systems because of its special growth characteristics or physiological structure, bring difficulty to application.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of decolouring bacterium, has colourity and COD ability in extremely strong removal paper waste.
Another technical problem to be solved by this invention is the application providing above-mentioned decolouring bacterium.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of decolouring bacterium, for acinetobacter calcoaceticus YH-02 (Acinetobacter sp.), its preserving number is CGMCC No.9413 (depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on 07 04th, 2014, deposit number: CGMCC No.9413).
Preferably, above-mentioned decolouring bacterium (acinetobacter calcoaceticus YH-02 (Acinetobacter sp.)), be separated in the mud of riverbank, Yihe, Linyi, getting mud diluent coats on screening culture medium flat board, cultivate in thermostat container, the single bacterium colony of picking repeats line purifying and obtains, screening flat board shows as white edge neat, moistening thickness, gramstaining is negative, identify through 16S rDNA, belong to acinetobacter calcoaceticus (Acinetobacter sp.), there is sequence shown in <400>1 in sequence table.
Above-mentioned decolouring bacterium, is removing the application in paper waste in colourity and COD
Preferably, the application of above-mentioned decolouring bacterium, utilizes described acinetobacter calcoaceticus YH-02 (Acinetobacter sp.), by this inoculation in described waste water, 30-35 DEG C of shaking culture 3-5 days, maintains dissolved oxygen at more than 3.0mg/L, and then realize the removal of paper waste decolouring and COD
Preferably, the application of above-mentioned decolouring bacterium, described acinetobacter calcoaceticus YH-02 (Acinetobacter sp.) bacterial strain is with the inoculation of bacterium liquid form, and its inoculum size is the 5-8% (v/v) of process wastewater volume.
Preferably, the application of above-mentioned decolouring bacterium, the concentration of described acinetobacter calcoaceticus YH-02 (Acinetobacter sp.) bacterial strain bacterium liquid is O.D
600≈ 2.0.
Preferably, the application of above-mentioned decolouring bacterium, specifically, described acinetobacter calcoaceticus YH-02 (Acinetobacter sp.) inoculation is in LB substratum, and 30 DEG C of shaking culture are dense to bacterium is O.D
600≈ 2.0, is inoculated in paper waste with the inoculum size of 5-8%, cultivates 3-5 days for 30-35 DEG C, maintains dissolved oxygen at more than 3.0mg/L; Centrifuging and taking supernatant, measures colourity according to " mensuration (GB11903-89) of water quality colourity ", measures COD according to " dichromate titration (GB11914-89) ".
The invention has the beneficial effects as follows:
The invention provides decolouring bacterium-acinetobacter calcoaceticus (Acinetobacter sp.) YH-02 (CGMCC No.9413), can carry out decolorization under aerobic condition, fast growth at heterotrophism, metabolic capacity is strong, and decolorizing efficiency is high; The ability of anti-various impact load is comparatively strong, more stable in the processing system, immobilization technology can be utilized to form microbial film, not easily run off and become feeble and die; All there is advantage than physical chemistry decoloring method and traditional decolouring microorganism, have wide practical use at sewage treatment area.
Accompanying drawing explanation
Fig. 1 is the systematic evolution tree of decolouring bacterium of the present invention, and in figure, Acinetobacter sp. is acinetobacter calcoaceticus YH-02.
Preservation information
Classification noun: acinetobacter calcoaceticus Acinetobacter sp.
Depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on 07 04th, 2014
Preserving number: CGMCC No.9413
Embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is further described.
Substratum different in following embodiment and solution formula as follows:
Bacterial classification used is in the following embodiments acinetobacter calcoaceticus YH-02 (Acinetobacter sp.), be one decolouring bacterium, preserving number: CGMCC No.9413, substratum different in embodiment and solution formula as follows:
1. screening culture medium: add the agar of 1.5% in paper waste, 121 DEG C of sterilizings 20 minutes, preparation screening culture medium is dull and stereotyped.
2.LB substratum: distilled water 1000mL, peptone 10.0g, yeast powder 5.0g, NaCl 5.0g, pH 7.0-7.2.
Embodiment 1
The screening and identification of acinetobacter calcoaceticus YH-02 (Acinetobacter sp.).
Experiment material is from riverbank, Yihe, Linyi mud.
Get mud diluent 0.1ml, coat on screening culture medium flat board, cultivate in 30 DEG C of thermostat containers, the single bacterium colony of picking repeats line purifying 2-3 time, obtains purifying bacterial strain.Identify through 16S rDNA, belong to acinetobacter calcoaceticus (Acinetobacter sp.), systematic evolution tree is shown in Figure of description 1, has sequence shown in <400>1 in sequence table.
Embodiment 2
The decolorization experiment 1 of acinetobacter calcoaceticus (Acinetobacter sp.) YH-02.
Handling object is paper waste, and operation scheme is that shake flask is cultivated.
Concrete implementation step is as follows: described inoculation is in LB substratum, and 30 DEG C of shaking culture are dense to bacterium is O.D
600≈ 2.0, the inoculum size with 8% is inoculated in paper waste, cultivates 5 days for 35 DEG C, maintains dissolved oxygen at more than 3.0mg/L; Centrifuging and taking supernatant, measures colourity according to " mensuration (GB11903-89) of water quality colourity ", measures COD according to " dichromate titration (GB11914-89) ".Result is as following table 1.
Table 1
| ? | Colourity (degree) | COD(mg/L) |
| Water inlet | 560 | 2800 |
| Water outlet | 40 | 274 |
| Clearance (%) | 92.9 | 90.2 |
Embodiment 3
The decolorization experiment 2 of acinetobacter calcoaceticus (Acinetobacter sp.) YH-02.
Handling object is paper waste, and operation scheme is that in 3L SBR (sequence bioreactor) system, aeration is cultivated.
Concrete implementation step is as follows: described inoculation is in LB substratum, and 30 DEG C of shaking culture are dense to bacterium is O.D
600≈ 2.0, the inoculum size with 5% is inoculated in paper waste, cultivates 3 days for 30 DEG C, maintains dissolved oxygen at more than 3.0mg/L; Centrifuging and taking supernatant, measures colourity according to " mensuration (GB11903-89) of water quality colourity ", measures COD according to " dichromate titration (GB11914-89) ".Result is as following table 2.
Table 2
| ? | Colourity (degree) | COD(mg/L) |
| Water inlet | 560 | 2800 |
| Water outlet | 60 | 397.6 |
| Clearance (%) | 89.3 | 85.8 |
Embodiment 4
The decolorization experiment 3 of acinetobacter calcoaceticus (Acinetobacter sp.) YH-02.
Handling object is paper waste, and operation scheme is that in 3L SBR system, aeration is cultivated.
Concrete implementation step is as follows: described inoculation is in LB substratum, and 30 DEG C of shaking culture are dense to bacterium is O.D
600≈ 2.0, the inoculum size with 8% is inoculated in paper waste, cultivates 5 days for 35 DEG C, maintains dissolved oxygen at more than 3.0mg/L; Centrifuging and taking supernatant, measures colourity according to " mensuration (GB11903-89) of water quality colourity ", measures COD according to " dichromate titration (GB11914-89) ".Result is as following table 3.
Table 3
| ? | Colourity (degree) | COD(mg/L) |
| Water inlet | 560 | 2800 |
| Water outlet | 30 | 187.6 |
| Clearance (%) | 94.6 | 93.3 |
As can be seen from above-mentioned experiment 1-3, decolouring bacterium-acinetobacter calcoaceticus (Acinetobacter sp.) YH-02 of the present invention can utilize the difficult degradations such as the Mierocrystalline cellulose in paper waste and xylogen to grow as substrate, remove the colourity in paper waste and COD efficiently, there is the double dominant of cost and operation simultaneously compared with chemical process, it is expected to this acinetobacter calcoaceticus and will make positive contribution for dirty water living creature processing technique.
Above-mentioned detailed description of this kind of bacterium of decolouring being carried out with application with reference to embodiment; illustrative instead of determinate; several embodiments can be listed according to institute's limited range, therefore in the change do not departed under general plotting of the present invention and amendment, should belong within protection scope of the present invention.
Claims (6)
1. a decolouring bacterium, is characterized in that: be acinetobacter calcoaceticus YH-02, its preserving number is CGMCC No.9413.
2. decolouring bacterium according to claim 1, it is characterized in that: be separated in the mud of riverbank, Yihe, Linyi, getting mud diluent coats on screening culture medium flat board, cultivate in thermostat container, the single bacterium colony of picking repeats line purifying and obtains, screening flat board shows as neat, the moistening thickness of white edge, gramstaining is negative, identify through 16S rDNA, belong to acinetobacter calcoaceticus Acinetobacter sp., there is sequence shown in <400>1 in sequence table.
3. the decolouring bacterium described in claim 1 or 2 is removing the application in paper waste in colourity and COD.
4. the application of decolouring bacterium according to claim 3, it is characterized in that: by described acinetobacter calcoaceticus YH-02 inoculation in described waste water, 30-35 DEG C of shaking culture 3-5 days, maintains dissolved oxygen at more than 3.0mg/L, and then realizes the removal of paper waste decolouring and COD.
5. the application of decolouring bacterium according to claim 3, is characterized in that: described acinetobacter calcoaceticus YH-02 bacterial strain is with the inoculation of bacterium liquid form, and its inoculum size is the 5-8% (v/v) of process wastewater volume.
6. the application of decolouring bacterium according to claim 3, is characterized in that: the concentration of described acinetobacter calcoaceticus YH-02 bacterial strain bacterium liquid is O.D
600≈ 2.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410597427.4A CN104312965B (en) | 2014-10-30 | 2014-10-30 | A kind of decolouring bacterium and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410597427.4A CN104312965B (en) | 2014-10-30 | 2014-10-30 | A kind of decolouring bacterium and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104312965A true CN104312965A (en) | 2015-01-28 |
| CN104312965B CN104312965B (en) | 2017-04-05 |
Family
ID=52368283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410597427.4A Expired - Fee Related CN104312965B (en) | 2014-10-30 | 2014-10-30 | A kind of decolouring bacterium and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104312965B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961633A (en) * | 2020-09-09 | 2020-11-20 | 闽江学院 | Acinetobacter with hexavalent chromium removal and decoloration functions, separation and purification method and application |
| CN112080450A (en) * | 2020-09-30 | 2020-12-15 | 内蒙古农业大学 | Straw degrading bacteria and separation screening method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888365A (en) * | 2012-09-17 | 2013-01-23 | 贾平 | Acinetobacter lwoffii and biological bacteria liquid pulping method thereof |
| CN103131637A (en) * | 2013-02-25 | 2013-06-05 | 黑龙江八一农垦大学 | Bacteria compsite inoculum used in lignin decomposition |
| CN104017753A (en) * | 2014-05-09 | 2014-09-03 | 西北农林科技大学 | Acinetobacter calcoaceticus capable of efficiently degrading lignin |
-
2014
- 2014-10-30 CN CN201410597427.4A patent/CN104312965B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888365A (en) * | 2012-09-17 | 2013-01-23 | 贾平 | Acinetobacter lwoffii and biological bacteria liquid pulping method thereof |
| CN103131637A (en) * | 2013-02-25 | 2013-06-05 | 黑龙江八一农垦大学 | Bacteria compsite inoculum used in lignin decomposition |
| CN104017753A (en) * | 2014-05-09 | 2014-09-03 | 西北农林科技大学 | Acinetobacter calcoaceticus capable of efficiently degrading lignin |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961633A (en) * | 2020-09-09 | 2020-11-20 | 闽江学院 | Acinetobacter with hexavalent chromium removal and decoloration functions, separation and purification method and application |
| CN112080450A (en) * | 2020-09-30 | 2020-12-15 | 内蒙古农业大学 | Straw degrading bacteria and separation screening method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104312965B (en) | 2017-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Siddharth et al. | Environmental applications of microbial extracellular polymeric substance (EPS): a review | |
| Talukdar et al. | Evaluation of novel indigenous fungal consortium for enhanced bioremediation of heavy metals from contaminated sites | |
| Adav et al. | Aerobic granular sludge: recent advances | |
| Hemambika et al. | Biosorption of heavy metals by immobilized and dead fungal cells: A comparative assessment | |
| Anand et al. | Bioaccumulation of copper by Trichoderma viride | |
| Agunbiade et al. | A Review of the Application of Biofloccualnts in Wastewater Treatment. | |
| Agunbiade et al. | Flocculating performance of a bioflocculant produced by Arthrobacter humicola in sewage waste water treatment | |
| Barbosa et al. | Nickel, manganese and copper removal by a mixed consortium of sulfate reducing bacteria at a high COD/sulfate ratio | |
| CN110079486B (en) | LAS and/or N degrading bacterium and application thereof | |
| CN104673730B (en) | One plant of Brevibacillus laterosporus and its application with fast degradation nitrite nitrogen function and bacteria resistance function | |
| Hasan et al. | Biosorption of manganese in drinking water by isolated bacteria | |
| CN103087945A (en) | Heavy-metal-resisting microbial agent, preparation method and application thereof | |
| CN104893999B (en) | A kind of enterobacteria and its application in lignin degrading | |
| CN102876574B (en) | Method for preparing oil-removing microbial inoculum and method for treating oil field sewage by using oil-removing microbial inoculum | |
| Sehar et al. | Evidence of microscopic correlation between biofilm kinetics and divalent cations for enhanced wastewater treatment efficiency | |
| CN108546656A (en) | One plant of salt tolerance pseudoalteromonas and its application | |
| Yan et al. | Sludge dewaterability enhancement under low temperature condition with cold-tolerant Bdellovibrio sp. CLL13 | |
| CN103833144B (en) | A kind of method utilizing flcos producing bacteria fermented liquid to remove heavy metal ion in water | |
| CN101429486B (en) | Brood cell bacillus MK3-1 for purification of heavy metal pollution and uses thereof | |
| CN104312965A (en) | Decoloration bacterium and application thereof | |
| Aravindhan et al. | Biological removal of carcinogenic chromium (VI) using mixed Pseudomonas strains | |
| CN107686172A (en) | A kind of preparation method of compound adsorbing metal ions flocculant | |
| CN112608857B (en) | Salt-tolerant bacillus pumilus SZG-NY-001 capable of repairing polluted water and application thereof | |
| CN101942407B (en) | Producing strain and production method for producing microbial flocculant from wheat starch waste water | |
| CN110951641B (en) | Bacillus subtilis, microbial agent and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170405 Termination date: 20181030 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |