CN104312561A - Application of composite microorganism bacterial in tertiary oil recovery - Google Patents
Application of composite microorganism bacterial in tertiary oil recovery Download PDFInfo
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- CN104312561A CN104312561A CN201410488914.7A CN201410488914A CN104312561A CN 104312561 A CN104312561 A CN 104312561A CN 201410488914 A CN201410488914 A CN 201410488914A CN 104312561 A CN104312561 A CN 104312561A
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- oil
- salt
- oil recovery
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
Abstract
The present invention discloses an application of a composite microorganism bacterial in tertiary oil recovery. According to the present invention, a mixed fermentation broth comprising three kinds of bacteria Halobacteriumsalinarium, Brevibacillusbrevis and Bacilluscereus according to a weight ratio of 1:1:1 is applied in tertiary oil recovery, the bacteria survive in a high salt oil recovery environment with a salt content of higher than 12%, the viscosities of the crude oil are respectively reduced by 15.4%, 26.4% and 35.2%, and the interfacial tension between the oil and the water are respectively reduced by 91.3%, 76.5% and 58.6%, such that the significant beneficial effects of reduction of the crude oil viscosity and reduction of the interfacial tension between the oil and the water are provided so as to provide wide application values.
Description
Technical field
The present invention relates to microorganism and field of microbial fermentation.Specifically, the present invention relates to the technical field applied in a kind of complex microorganism bacterial classification application tertiary process.
Background technology
On the earth except wide ocean (saliferous 3.5-3.8%), land is also dispersed with salt lake, many salt ponds and salt alkali lake.Well-known Dead Sea saltiness is just up to 31.5%.China has wide salt territory environment and than more rich halophilic microorganism resource.There is the salt lake and salt alkali lake that the saliferous of One's name is legion is very high on the ground such as China Tibet, Qinghai, Xinjiang and the Inner Mongol.Investigation finds that these salt territory environment have microorganism growth, namely there is halophilic bacterium.On microbiology category, halophilic bacterium is often referred to Natrinema altunense sp, and namely the suitableeest salt concn is greater than the microorganism of 3.0mol/L, and the most chemoheterotrophy of halophilic bacterium, cell contains carotenoid, Gram-negative, and thalline has color usually.Published in 1989 "
bergey, s Manual of Systematic Bacterio-logy" in the third edition, halophilic bacterium is decided to be 6 genus.
The microorganism lived under the very high environment of saliferous be called as halophilic bacterium (
halophiles), according to different addicted to salt degree, slight halophilic bacterium (the suitableeest salt concn is 0.2-0.5mol/L), Halophilic Bacterium (the suitableeest salt concn is 0.5-2.0mol/L) and Natrinema altunense sp (the suitableeest salt concn>=3.0mol/L) can be divided into.Wherein Natrinema altunense sp is halophilic archaea.Show that according to biological chemistry in recent years, molecular biology and genetics research halophilic bacterium and methanobacteria and be different from prokaryotic organism addicted to sour thermophile bacteria are different from eukaryote again, belong to the 3rd class form of life-archeobacteria (
archeabacteria).
Hypersaline environment is compared with common salt-free or low-salt environment, very high salt ionic concentration in environment proposes very high requirement to microorganism cells osmotic pressure, the cell walls of halophilic bacterium, cytolemma have special physiological function, film has special lipoid substance, as phosphatidyl glycerol analogue, two phytane ethers, sulfonic-glycerol fat etc.There is inside and outside film the salt of different sorts and concentration, and maintain stability and the cytoactive of membrane structure by salt.Halophilic bacterium, by assembling k ion and small molecules polar material under hypersaline environment in born of the same parents, regulates Premeabilisation of cells pressure, maintains osmotic balance inside and outside born of the same parents, makes cell can obtain moisture from environment.Containing excessive acidic amino acid and nonpolar resistates in the protein of halophilic bacterium, excessive acidic substance need the negative charge near cation balance, so only could keep active addicted to salt enzyme in hypersaline environment.
The biochemical composition of the viability of halophilic bacterium uniqueness and the interior uniqueness of cell and structure, cause and study interest widely.At present the research of halophilic bacterium application aspect is concentrated on to the enzyme that they are produced, the aspects such as some functional moleculars, high polymer and biological environmental production.The enzyme that halophilic bacterium produces is the important sources of industrial salt tolerant enzyme, and some halophilic bacterium can accumulate PHB under certain condition in a large number, for the exploitation of degradable biomaterial.In addition halophilic bacterium also has the report of application in the process of high salt sewage.
Microbial Enhanced Oil Recovery refers to and the microorganism of screening or microbial metabolites is injected oil reservoir, acts on crude oil, changes some physicochemical characteristic of crude oil, thus improve the technology of oil recovery factor through the vital movement of microorganism or some characteristic of meta-bolites.Since 20 middle of century carry out the research of Microbial Enhanced Oil Recovery; after the nineties each oil field of China all in various degree carried out the field test of mass-producing; Microbial Enhanced Oil Recovery has now developed into the important new and high technology in tertiary oil recovery; because oil recovery geological condition is complicated; for each different reservoir media, the key of Microbial Enhanced Oil Recovery is adopted to be to screen the microorganism of applicable this area's oil recovery environment and to study the cultivation and zymotechnique that are applicable to this quasi-microorganism.Due to mining site circumstance complication, the oil reservoir of current not all condition all can use microbe oil production, it is key constraints, wherein high well temperature environment that its restricted condition mainly contains well temperature, salinity and pH value etc., has had the precedent adopting thermophile bacteria to be used successfully to high-temperature oil reservoir.But be greater than 10*10 for salinity
4it is 2.5mol/L that mg/L(amounts to NaCl content) high salinity reservoir, there is no effective oil extraction microbial bacterial classification application research report.
Summary of the invention
10*10 is greater than for salinity for domestic and international Microbial Enhanced Oil Recovery at present
4it is 2.5mol/L that mg/L(amounts to NaCl content) high salinity reservoir, there is no effective oil extraction microbial composite bacteria application tertiary process application present situation, the present invention proposes this in a kind of complex microorganism bacterial classification application tertiary process and applies.
The present invention specifically provides in a kind of complex microorganism bacterial classification application tertiary process and applies, employing sabkha salt bacillus (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium are applied in tertiary process according to weight ratio 1:1:1 mixed fermentation liquid, survive in high salt oil production environment saltiness is higher than the hypersaline environment of 12%, its crude oil reduces Crude viscosity and reaches 15.4%, 26.4%, 35.2% respectively, reduces interfacial tension between profit and reaches 91.3%, 76.5%, 58.6% respectively.There is the beneficial effect significantly reducing Crude viscosity and reduce interfacial tension between profit.
In the present invention, the sabkha salt bacillus of employing (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium all buy by domestic and international public's channel and obtain, but prior art has no and adopts these three kinds of bacterium to be compounded in tertiary process to apply.
By implementing the concrete technical scheme of the present invention, realizing content of the present invention, and following beneficial effect can be reached.
The invention provides in a kind of complex microorganism bacterial classification application tertiary process and apply, employing sabkha salt bacillus (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium are applied in tertiary process according to weight ratio 1:1:1 mixing, by the indoor microbe oil production performance evaluation to multiple different high salinity reservoir, complex microorganism bacterial classification fermented liquid provided by the invention is used as oil extraction microbial in hypersaline environment, when with the addition of nutritive medium, the crude oil that environment saltiness is 4% is not reduced to Crude viscosity and reduces the effect of interfacial tension between profit, and can not survive.Survive in high salt oil production environment saltiness is higher than the hypersaline environment of 12%, its crude oil reduces Crude viscosity and reaches 15.4%, 26.4%, 35.2% respectively, reduces interfacial tension between profit and reaches 91.3%, 76.5%, 58.6% respectively.There is the beneficial effect significantly reducing Crude viscosity and reduce interfacial tension between profit.
accompanying drawing illustrates (nothing)
, for embodiment, the present invention is described below, but the present invention is not limited to following embodiment.In addition, in following explanation, if no special instructions, then % all refers to that weight is write.
Embodiment
embodiment one: apply in complex microorganism bacterial classification application tertiary process
Sabkha salt bacillus (
halobacterium salinarium) separation of Hab-g03
Employing sabkha salt bacillus (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium apply in tertiary process according to weight ratio 1:1:1 mixed fermentation liquid.
In the present invention, the sabkha salt bacillus of employing (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium all buy by domestic and international public's channel and obtain, the acquisition of fermented liquid is also visible by prior art, but prior art has no employing, and these three kinds of bacterium composite fermentation liquids are applied in tertiary process.
embodiment two: application effect appraisal
Employing sabkha salt bacillus that above-described embodiment is provided (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium evaluate in the adaptability of high salt oil production environment and oil recovery effect according to weight ratio 1:1:1 mixed fermentation liquid; Displacement Efficiency has been carried out to the crude oil on the ground such as oil producing region, Kelamayi hypersaline environment and Qinghai Hua Tugou picacho; evaluation index has: Crude viscosity reduces, interfacial tension lowering between crude oil and local water and possess growth tolerance test; test method: add 60ml crude oil in airtight container; 90ml local water; and extreme halotolerant fermented liquid 1.5ml; add 3ml nutrient solution; at 35 DEG C, 8d cultivated by shaking table.Measure viscosity of crude in evaluation method and use rotational viscosimeter, in test of the present invention, use viscosity to count the NDJ-5S type Digital Viscometer of Shanghai Jing Ke Industrial Co., Ltd..Between profit, the measurement of interfacial tension uses around-France measurement, and in test of the present invention, interfacial tensimeter used is the JZHY-180 type interfacial tensimeter of Chengde experimental machine company limited.The flat board that growth tolerance test adopts concentration and separation substratum made, is separated by line the growth tolerance that halophilic bacterium confirms halophilic bacterium in evaluation test high salt oil production environment.
Table 1: complex microorganism bacterial classification fermented liquid recovers the oil at high salt oil production environmental microorganism and applies
The list of effect assessment test-results
As seen from Table 1, complex microorganism bacterial classification provided by the invention is used as oil extraction microbial in hypersaline environment, when with the addition of nutritive medium, the crude oil that environment saltiness is 4% not being reduced to Crude viscosity and reducing the effect of interfacial tension between profit, and can not survive.Can survive in high salt oil production environment, three kinds of saltiness listed in his-and-hers watches reach 15.4%, 26.4%, 35.2% respectively higher than the crude oil reduction Crude viscosity of the hypersaline environment of 12%, reduce interfacial tension between profit and reach 91.3%, 76.5%, 58.6% respectively.There is the beneficial effect significantly reducing Crude viscosity and reduce interfacial tension between profit.Illustrate that complex microorganism bacterial classification provided by the invention is suitable for and is only applicable to hypersaline environment microbe oil production.
Claims (1)
1. apply in a complex microorganism bacterial classification application tertiary process, it is characterized in that, employing sabkha salt bacillus (
halobacterium salinarium), bacillus brevis (
brevibacillus brevis) and bacillus cereus (
bacilluscereus) three kinds of bacterium apply in tertiary process according to weight ratio 1:1:1 mixed fermentation liquid.
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| CN201410488914.7A CN104312561A (en) | 2014-09-23 | 2014-09-23 | Application of composite microorganism bacterial in tertiary oil recovery |
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| CN201410488914.7A CN104312561A (en) | 2014-09-23 | 2014-09-23 | Application of composite microorganism bacterial in tertiary oil recovery |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106467731A (en) * | 2016-08-30 | 2017-03-01 | 雷春生 | A kind of method that fermentable prepares thinner |
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2014
- 2014-09-23 CN CN201410488914.7A patent/CN104312561A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106467731A (en) * | 2016-08-30 | 2017-03-01 | 雷春生 | A kind of method that fermentable prepares thinner |
| CN106467731B (en) * | 2016-08-30 | 2019-02-19 | 克拉玛依市新奥达石油技术服务有限公司 | A kind of method that microbial fermentation prepares thinner |
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Application publication date: 20150128 |
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