CN104306424A - Application of semen lepidii fatty oil in preparing drugs capable of suppressing cough, dispelling phlegm and calming panting - Google Patents

Application of semen lepidii fatty oil in preparing drugs capable of suppressing cough, dispelling phlegm and calming panting Download PDF

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CN104306424A
CN104306424A CN201410349621.0A CN201410349621A CN104306424A CN 104306424 A CN104306424 A CN 104306424A CN 201410349621 A CN201410349621 A CN 201410349621A CN 104306424 A CN104306424 A CN 104306424A
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fatty oil
semen
semen lepidii
lepidii
descurainiae
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郑晓珂
赫金丽
弓建红
张明辉
张国顺
张娜
袁培培
李玲玲
孙亚萍
王小兰
冯卫生
匡海学
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Henan University of Traditional Chinese Medicine HUTCM
Heilongjiang University of Chinese Medicine
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Heilongjiang University of Chinese Medicine
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    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K2236/30—Extraction of the material
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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Abstract

The invention discloses an application of semen lepidii fatty oil in preparing drugs capable of suppressing cough, dispelling phlegm and calming panting. The semen lepidii fatty oil is used for preparing the drugs capable of suppressing the cough, dispelling the phlegm and calming the panting. Compared with conventional traditional Chinese medicines, the semen lepidii fatty oil has the following beneficial effects: (1) experiments demonstrate that the semen lepidii fatty oil component can obviously prolong an incubation period of cough and reduce times of the cough, indicating that the semen lepidii fatty oil component has an effect of suppressing the cough; the semen lepidii fatty oil component can significantly increase mice tracheal phenol red output, indicating that the semen lepidii fatty oil component has an effect of dispelling the phlegm; and that the semen lepidii fatty oil component can obviously prolong an incubation period of guinea pig asthma reaction, indicating that the semen lepidii fatty oil component has an effect of calming the panting, so that novel medicinal applications of the semen lepidii fatty oil are developed; and (2) the conventional traditional Chinese medicines are usually taken by water decoction, which is time-consuming and labor-consuming and is not convenient to take or carry by people, but the semen lepidii fatty oil is small in dosage and convenient to take, effectively solves drug problems of respiratory tract related diseases, and has enormous clinical significance.

Description

The application of Semen Lepidii (Semen Descurainiae) fatty oil in preparation treatment eliminating phlegm and stopping cough suppressing panting calming medicine
Technical field
The invention belongs to the application of natural plants, be specifically related to the application of Semen Lepidii (Semen Descurainiae) fatty oil in preparation treatment eliminating phlegm and stopping cough suppressing panting calming medicine.
Background technology
Semen Lepidii (Semen Descurainiae) be crucifer Lepidium sativum L. ( lepidiumapetalumwilld or descurainia sophia (l.) webb ex prantl descuriniasophia(L.) seed of the drying and ripening of Webb exPrantl, the former is called lepidii,semen; The latter is called Semen Descurainiae.Begin to be loaded in Shennong's Herbal, acrid in the mouth is bitter, property Great Cold, returns lung, urinary bladder channel.For traditional conventional Chinese medicine, there is effect of eliminating pathogen from the lung for relieving asthma, inducing diuresis to remove edema, heart tonifying.Be usually used in treating accumulation and obstruction of sputum, must not pant and put down sleeping, edema, hydrothorax, breast abdomen hydrops, dysuria etc.Modern pharmacological research shows, Semen Lepidii (Semen Descurainiae) Aqueous extracts obviously can improve the retention of sodium and water of Heart Failure Wistar Rats, and has significant diuresis.Wherein, be that the compound recipe Semen Lepidii (Semen Descurainiae) capsule of principal agent has certain phlegm-dispelling functions with Semen Lepidii (Semen Descurainiae), and stimulate the mouse cough reaction caused also to have obvious antitussive action to ammonia mist, its effect strengthens with the increase of dosage.In addition, bibliographical information Semen Lepidii (Semen Descurainiae) is had to have the effects such as certain antioxidant activity, adjusting blood lipid.But not yet there is the research report in Semen Lepidii (Semen Descurainiae) fatty oil is active so far.
Semen Lepidii (Semen Descurainiae) all has cultivation in China's major part provinces and regions, and resource is very abundant, and its seed production is comparatively large, and in China always by as Chinese medicine, be usually used in clinically treating various diseases, its material base of curing the disease determines containing unique activity composition.Chemical composition kind contained by Semen Lepidii (Semen Descurainiae) is more, now be separated from Lepidium sativum L. and descurainia sophia (l.) webb ex prantl and obtained cardiac glycoside, isothiocyanic acid and sulfur glycoside, fatty oils, alkaloids, flavonoid, phenolic acids, the chemical compositions such as Coumarins, modern pharmacological research shows Semen Lepidii (Semen Descurainiae), diuresis, the effects such as Adjust-blood lipid, but not yet have so far and play the effective site of eliminating phlegm and stopping cough antiasthmatic effect and the research report of effective ingredient about Semen Lepidii (Semen Descurainiae), not report in clear and definite Semen Lepidii (Semen Descurainiae) chemical composition the effect which kind of or which class has eliminating phlegm and stopping cough and relievings asthma, thus, current Semen Lepidii (Semen Descurainiae) generally adopts soak by water method when eliminating phlegm and stopping cough relievings asthma medication, not only consuming time, effort, and take, it is all not too convenient to carry, inconvenience is brought to patient medication.
Summary of the invention
For exist in prior art because not having in clear and definite Semen Lepidii (Semen Descurainiae) which kind of or which class chemical composition have cough-relieving, eliminate the phlegm, the problem of the inconvenience that the effect of relievining asthma brings to patient medication, the invention provides the application of Semen Lepidii (Semen Descurainiae) fatty oil in preparation treatment eliminating phlegm and stopping cough suppressing panting calming medicine, clearly show: in Semen Lepidii (Semen Descurainiae) chemical composition, fatty oil component has the effect that good eliminating phlegm and stopping cough is relievingd asthma, facilitate the application of Semen Lepidii (Semen Descurainiae) in clinical treating disease.
For achieving the above object, the present invention is by the following technical solutions:
Semen Lepidii (Semen Descurainiae) fatty oil is preparing the application in eliminating phlegm and stopping cough suppressing panting calming medicine, and described Semen Lepidii (Semen Descurainiae) fatty oil is for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.
Described Semen Lepidii (Semen Descurainiae) fatty oil be by weigh after Semen Lepidii (Semen Descurainiae) be crushed to 50-70 order through pulverizer, Semen Lepidii (Semen Descurainiae) after pulverizing is placed in container, then in container, add Semen Lepidii (Semen Descurainiae) weight 8-12 petroleum ether doubly, reflux, extract, twice at temperature is 60-90 DEG C, each reflux, extract, 0.4-0.6 hour, extracts after terminating, extracting solution filter paper sucking filtration at a slow speed, filtrate is used rotary evaporator decompression and solvent recovery, oil sample is revolved and steams to constant weight, namely obtain Semen Lepidii (Semen Descurainiae) fatty oil; Described Semen Lepidii (Semen Descurainiae) fatty oil is for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.
The present invention adopts water boiling and extraction method to combine extraction, precipitate with ethanol, the technology such as macroporous adsorbent resin (DiaionHP-20 macroporous adsorbent resin), Semen Lepidii (Semen Descurainiae) decocting liquid is split as 6 positions non-cross between composition: volatile oil compositions, petroleum ether extraction component (fatty oil component), precipitate with ethanol component (polysaccharide component), 20% ethanol elution component, 80% ethanol elution component, water elution component, each position is carried out to the screening of eliminating phlegm and stopping cough antiasthmatic activity, find that fatty oil component has good eliminating phlegm and stopping cough antiasthmatic effect, therefore, its eliminating phlegm and stopping cough antiasthmatic effect is further inquired into.
The present invention adopts ammonia to lure the method for coughing, mice PSP method, and Cavia porcellus spraying causes the method for breathing heavily, and observes the cough-relieving of Semen Lepidii (Semen Descurainiae) fatty oil, eliminates the phlegm, antiasthmatic effect; Experimental result shows: Semen Lepidii (Semen Descurainiae) fatty oil obviously can extend mouse cough incubation period, reduce cough number of times, points out it to have antitussive action; Semen Lepidii (Semen Descurainiae) fatty oil component significantly can increase mice trachea phenols contents, points out it to have phlegm-dispelling functions; Semen Lepidii (Semen Descurainiae) fatty oil component can obvious prolonged guinea pig asthma reaction incubation period, and prompting Semen Lepidii (Semen Descurainiae) fatty oil component has antiasthmatic effect; Thus specify that the effect that Semen Lepidii (Semen Descurainiae) fatty oil component has eliminating phlegm and stopping cough and relievings asthma.
Compared with prior art, beneficial effect of the present invention is: 1. Semen Lepidii (Semen Descurainiae) fatty oil preparation method of the present invention is simple, convenient, cost is low, reliable and stable, and tests more than repeatedly, all achieves identical or akin result; 2. show by experiment: Semen Lepidii (Semen Descurainiae) fatty oil component obviously can extend cough latent period, reduce cough number of times, shows that it has antitussive action; Semen Lepidii (Semen Descurainiae) fatty oil component significantly can increase mice trachea phenols contents, shows that it has phlegm-dispelling functions; In addition, Semen Lepidii (Semen Descurainiae) fatty oil component can obvious prolonged guinea pig asthma reaction incubation period, shows that Semen Lepidii (Semen Descurainiae) fatty oil component has antiasthmatic effect, thus opens the medicinal novelty teabag of Semen Lepidii (Semen Descurainiae) fatty oil; 3. Chinese medicine generally adopts soak by water method, and not only consuming time, effort, and people is taken, it is all not too convenient to carry; And with Semen Lepidii (Semen Descurainiae) fatty oil, not only consumption is few, taking convenience, and effectively solves the medication problem of diseases concerned with respiratory, and clinical meaning is huge, has good DEVELOPMENT PROSPECT.
Accompanying drawing explanation
Fig. 1 is the process chart that in embodiment 1, each component of Semen Lepidii (Semen Descurainiae) splits.
Fig. 2 be water elution component in embodiment 1,20%, 80% ethanol elution component sample clustering analysis chart.
Fig. 3 be water elution component in embodiment 1,20%, 80% ethanol elution component sample principal component analysis figure.
Fig. 4 be water elution component in embodiment 1,20%, the orthogonal Partial Least Squares Method figure of 80% ethanol elution component sample.
Fig. 5 is the total ion current figure of fatty oil component in embodiment 1.
Detailed description of the invention
embodiment 1
The material that the present embodiment is used and instrument:
1. experiment material
1.1 animal
Kunming mouse, male and female half and half, body weight 18-22g; Cavia porcellus, male and female half and half, body weight 180-220g; Credit number: scxk Shandong 20130001 (being provided by the anti-medical joint-stock company in Shandong, Shandong Product Quality Verification Centers).Raise in 18 ~ 22 DEG C of cleaning grade zooperies, free diet, drinking-water.
1.2 medicines and reagent
Semen Descurainiae (being purchased from Zhengzhou City's Chinese Medicinal Materials Markets, through being accredited as the dry seed of crucifer descurainia sophia (l.) webb ex prantl Descurainiasophia (L.) Webb. ex Prantl.) awards qualification by Henan College Of Traditional Chinese Medicine Dong Cheng penetrating judgment; Histamine phosphate, product batch number A0165, Shanghai Kai Yang Bioisystech Co., Ltd provides; Acecoline, product batch number G8320, Beijing Suo Laibao Science and Technology Ltd. provides; Ammonia, analytical pure, lot number: 20111013, Yantai City in pairs Chemical Co., Ltd. provides.
1.3 key instrument
Agilent7890/7000B triplex tandem level Four bar gas chromatograph-mass spectrometer (Agilent company of the U.S.), AB204-N type ten thousand/electronic balance (Henan Ai Bote development in science and technology company limited), Q-250A3 pulverizer (Rui Da is international), SB-1000 type rotary evaporator (Shanghai Ai Lang Instrument Ltd.), Bio Mate 3S ultraviolet spectrophotometer (Thermo Fisher Scientific), YLS-8A is multi-functional to be lured to cough to draw and breathes heavily instrument (Jinan Yi Yan development in science and technology company limited), SK6200H type ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.), DFZ-3 type vacuum drying oven (Shanghai Medical constant-temperature equipment company limited), DRT-ZN type electric jacket (Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.), condensing unit (Longchang, Sichuan Province glass apparatus factory), ChemPattern software (Ke Maien Beijing Science and Technology Ltd.).
The present embodiment adopts water boiling and extraction method to combine the technology such as extraction, precipitate with ethanol, macroporous adsorbent resin (DiaionHP-20 macroporous adsorbent resin), Semen Lepidii (Semen Descurainiae) decocting liquid is split as 6 positions non-cross between composition: volatile oil compositions, petroleum ether extraction component (fatty oil component), precipitate with ethanol component (polysaccharide component), 20% ethanol elution component, 80% ethanol elution component, water elution component, technological process as shown in Figure 1.
The present embodiment is to the non-cross research of Semen Lepidii (Semen Descurainiae) each component ingredient: because of fatty oil component, volatile oil compositions, between polysaccharide component with water-soluble component, composition can not intersect, likely intersect be water elution component, 20%, 80% ethanol elution component, therefore, by the HPLC-DAD data importing ChemPattern software of component, complementary chiasma research is carried out to the component of likely intersecting.
HPLC-DAD data clusters is analyzed: by 8 batches of test samples preparing by the analysis of Semen Lepidii (Semen Descurainiae) decocting liquid HPLC-DAD liquid-phase condition sample introduction, cluster analysis result is divided into 3 large classes, No. 1 ~ 8, sample be a class, 9 ~ No. 17 be a class, 18 ~ No. 24 be a class.Demonstrate water elution component, 20%, each poly-class of 80% ethanol elution component, non-cross, analysis result is as shown in Figure 2.
HPLC-DAD data principal component analysis: by 8 batches of test samples preparing by Semen Lepidii (Semen Descurainiae) decocting liquid by the analysis of HPLC-DAD liquid-phase condition sample introduction, record 100min chromatogram, analyze through ChemPattern software processes, result is as shown in Figure 3.
The orthogonal partial least square method of HPLC-DAD data: on the basis of preliminary analysis, carry out again the analysis of orthogonal partial least square method, obtain result, see Fig. 4, find from LV1-LV2-LV3 scatterplot, sample can obviously be divided into 3 regions, and No. 1 ~ 8, sample forms No. I, 9 ~ 17, region and forms formation region III, No. II, 18 ~ 24, region.Demonstrate water elution component, 20%, 80% ethanol elution component is non-cross, result is as shown in Figure 4.
Each position is carried out to the screening of eliminating phlegm and stopping cough antiasthmatic effect, find that fatty oil component has good eliminating phlegm and stopping cough antiasthmatic effect, therefore, the eliminating phlegm and stopping cough antiasthmatic effect of Semen Lepidii (Semen Descurainiae) fatty oil is further inquired into.
The present embodiment Semen Lepidii (Semen Descurainiae) fatty oil is by weighing 500g Semen Lepidii (Semen Descurainiae), through pulverizer, the Semen Lepidii (Semen Descurainiae) after weighing is crushed to 60 orders, Semen Lepidii (Semen Descurainiae) after pulverizing is placed in container, in container, then add the petroleum ether of Semen Lepidii (Semen Descurainiae) weight 10 times, reflux, extract, twice at temperature is 90 DEG C, each reflux, extract, 0.5 hour, after extraction terminates, extracting solution filter paper sucking filtration at a slow speed, uses rotary evaporator decompression and solvent recovery by filtrate, oil sample is revolved and steams to constant weight, namely obtain Semen Lepidii (Semen Descurainiae) fatty oil; By obtained Semen Lepidii (Semen Descurainiae) fatty oil for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.
the present embodiment ammonia stimulates to draw coughs experiment:
Kunming mouse, male and female half and half, body weight 18-22g; Credit number: scxk Shandong 20130001 (being provided by the anti-medical joint-stock company in Shandong, Shandong Product Quality Verification Centers); Raise in 18 ~ 22 DEG C of cleaning grade zooperies, free diet, drinking-water.
Get mice to be put in one by one in bell glass, draw cough 15s with 25% ammonia spraying, observation mice to there is the time that contraction of abdominal muscle is opened one's mouth, does not occur in 1.5min that cough person discards from spraying.Select responsive Kunming mouse 20, male and female half and half, be divided into 2 groups at random by body weight and sex, often organize 10, be respectively blank group, Semen Lepidii (Semen Descurainiae) fat line of oils.Blank group gives same volume distilled water, and Semen Lepidii (Semen Descurainiae) fat line of oils dosage is by people and mice 20 times of dose,equivalent conversions, and administration volume is 1.0ml/100g.Daily 1 time, continuous 7 days.Ad lib, drinking-water during administration.After last administration, mice is put into bell glass by 30min, draws and coughs 15s, receive or contracting breast with mice abdominal muscle with 25% ammonia spraying, magnifies mouth for cough index simultaneously, observes each group of mice self-spray and starts the cough number of times to the incubation period and 2min of appearance cough.
The present embodiment Semen Lepidii (Semen Descurainiae) fatty oil draws the impact coughing experiment on mice ammonia:
Compare with blank group, Semen Lepidii (Semen Descurainiae) fat line of oils obviously can extend cough latent period, reduce cough number of times, all there is significant difference (P<0.01, P<0.05), show that Semen Lepidii (Semen Descurainiae) fatty oil has antitussive action.The results are shown in Table 1.
Table 1 Semen Lepidii (Semen Descurainiae) fat line of oils on mice ammonia draw cough experiment impact ( ± s, n=10)
Note: compare with blank group, * represents P<0.05, * * represents P<0.01.
the phenol red experiment of the present embodiment mice trachea excretion:
Kunming mouse, male and female half and half, body weight 18-22g; Credit number: scxk Shandong 20130001 (being provided by the anti-medical joint-stock company in Shandong, Shandong Product Quality Verification Centers); Raise in 18 ~ 22 DEG C of cleaning grade zooperies, free diet, drinking-water.
Kunming mouse 20, male and female half and half, are divided into 2 groups at random by body weight and sex, often organize 10, are respectively blank group, Semen Lepidii (Semen Descurainiae) fat line of oils.Blank group gives same volume distilled water, and dosage is by people and mice 20 times of dose,equivalent conversions, and administration volume is 1.0ml/100g.Daily 1 time, continuous 7 days.After last administration 30min, the phenol red solution (0.1ml/10g) of lumbar injection 5%, mice is put to death after injecting phenol red 30 min, cut off skin of neck, careful separation trachea (avoiding hemorrhage in operating process), peel off trachea surrounding tissue, clip from thyroid cartilage trachea to one section of trachea of trachea bifurcation.Put in the test tube filling 2 mL 0.9% sodium chloride, add 1 mol/L NaOH solution 0.1 mL again, absorbance is surveyed in wavelength 546 nm afterwards with ultraviolet spectrophotometer, do a standard curve with concentration known phenol red solution, calculate phenol red content (μ g/ml) in mice trachea according to curve and compare with blank group.
the preparation of phenol red standard curve: take a certain amount of phenol red by ten thousand/(analysis) balance precisions, dissolve with 5% sodium bicarbonate, be made into 1ml and contain the phenol red solution of 100ug, then be diluted to successively every milliliter containing phenol red 0.1,0.3,0.7,1,3,5,10ug, with 5% sodium bicarbonate solution for blank, with spectrophotometer at 546nm place, survey absorbance, each concentration parallel assay 3 times, get its meansigma methods, with phenol red dosage for abscissa, absorbance is vertical coordinate, obtains phenol red standard curve equation Y=0.1387X+0.0006(r=0.9998).
The present embodiment Semen Lepidii (Semen Descurainiae) fatty oil is on the impact of mice trachea phenols contents:
Compare with blank group, Semen Lepidii (Semen Descurainiae) fat line of oils significantly can increase mice trachea phenols contents, has significant difference (P<0.05), shows that Semen Lepidii (Semen Descurainiae) fatty oil has phlegm-dispelling functions.The results are shown in Table 2.
Table 2 Semen Lepidii (Semen Descurainiae) fatty oil on the phenol red output impact of mice trachea ( ± s, n=10)
Note: compare with blank group, * represents P<0.05, * * represents P<0.01.
the present embodiment Cavia porcellus is relievingd asthma experiment:
Cavia porcellus, male and female half and half, body weight 180-220g; Credit number: scxk Shandong 20130001 (being provided by the anti-medical joint-stock company in Shandong, Shandong Product Quality Verification Centers).Raise in 18 ~ 22 DEG C of cleaning grade zooperies, free diet, drinking-water.
Get the healthy guinea pig some of body weight (180-200) g, male and female half and half.Respectively Cavia porcellus being put volume is in 4L aerosol case, after Cavia porcellus peace and quiet, start air compressor, the equal-volume mixed liquor of 2% acecoline and 0.1% histamine phosphate is sprayed into the constant voltage of 400mmHg, each 15s, after stopping spraying, in observation 6min, asthmatic latent period is (to asthma attack, dyspnea from spraying, directly only twitch the time of falling), if occur in 150s, asthmatic tiqueur is qualified for screening.Select qualified Cavia porcellus 16, male and female half and half, divide 2 groups at random according to body weight, be respectively blank group, Semen Lepidii (Semen Descurainiae) fat line of oils.Blank group gives isopyknic distilled water; Medication all adopts gastric infusion, and administration volume is 1.0ml/100g, successive administration 5 days.After administration in 5th day after 1h, again insert in aerosol case, method is the same, observes and record each Mus asthmatic tic incubation period.
The present embodiment Semen Lepidii (Semen Descurainiae) fatty oil is on the impact of the caused Experimental Asthma In Guinea-pigs of spraying:
Compare with blank group matched group, Semen Lepidii (Semen Descurainiae) fatty oil component can obvious prolonged guinea pig asthma reaction incubation period, and significant difference (P<0.05), shows that Semen Lepidii (Semen Descurainiae) fatty oil component has antiasthmatic effect.The results are shown in Table 3.
Table 3 Semen Lepidii (Semen Descurainiae) fatty oil component on the impact of Experimental Asthma In Guinea-pigs ( ± s, n=8)
Note: compare with blank group, * represents P<0.05, * * represents P<0.01.
Above-mentioned experimental result shows: Semen Lepidii (Semen Descurainiae) fatty oil component obviously can extend cough latent period, reduce cough number of times, shows that it has antitussive action; Semen Lepidii (Semen Descurainiae) fatty oil component significantly can increase mice trachea phenols contents, shows that it has phlegm-dispelling functions; In addition, Semen Lepidii (Semen Descurainiae) fatty oil component can obvious prolonged guinea pig asthma reaction incubation period, shows that Semen Lepidii (Semen Descurainiae) fatty oil component has antiasthmatic effect, thus opens the medicinal novelty teabag of Semen Lepidii (Semen Descurainiae) fatty oil.
The qualification of the present embodiment Semen Lepidii (Semen Descurainiae) fatty oil component:
Get the present embodiment Semen Lepidii (Semen Descurainiae) fatty oil appropriate (about 100mg) in 10mL tool plug scale test tube, add 4mL normal hexane, then add 1mL0.8mol L -1sodium hydrate methanol solution, vortex 5min, add the drying of 0.5g sodium bisulfate, centrifugal 10min, gets the supernatant, crosses 0.22 μm of filter membrane, carries out GC-MS analysis.GC conditions: chromatographic column is DB-5 type quartz capillary column (30m × 0.25mm, 0.25 μm), and carrier gas is high-purity helium (99.999%), helium flow velocity 1.0mL min -1; Injector temperature is 280 DEG C; Split sampling, split ratio 10:1, sample size 0.1 μ L; Heating schedule is 50 DEG C, keeps 2 minutes, then by 3 DEG C of min -1be warming up to 200 DEG C, with 20 DEG C of min -1be warming up to 300 DEG C, keep 5min, vaporizer temperature 280 DEG C, interface temperature 280 DEG C.Mass Spectrometry Conditions: impinging air flows speed: helium 2.25ml min -1; Nitrogen 1.5ml min -1; Ion source: electron bombardment ionization source, electron energy 70eV; Sweep limits 45 ~ 400amu, level Four bar temperature 150 DEG C, ion source temperature 230 DEG C, solvent delay: 4.0min, GC-MS interface temperature 280 DEG C, analysis result is as shown in Figure 5.
Mass spectral database retrieval is carried out to the mass spectrum at each peak in total ion current figure, choose front 10 possibility materials that similarity is high, obtain its retention index KI respectively by http://webbook.nist.gov/chemistry/ search document (DB-5 post) and be worth literature value, tentatively determine chemical composition.Retention index KI result by references is compared with retention index KI result of calculation, with mass spectrum similarity and the highest chemical constitution of retention index KI value nearness for best qualification result, the part that cannot obtain retention index is still as the criterion with similarity, use areas of peak normalization method to obtain the percentage contents of each volatile component, result is as table 4: table 4 Semen Lepidii (Semen Descurainiae) fatty oil chemical composition GC-MS analysis result simultaneously
Analyze Semen Descurainiae fatty oil component separation detection by GC-MS and go out 64 components, 54 kinds of main components are identified with the dual method qualitatively of retention index through mass spectral database retrieval, its relative amount summation accounts for and always detects 97.75% of compound, the material that content enriches the most is 9, 12-octadecadienoic methyl (9, 12-Octadecadiynoic acid, methyl ester), account for 21.58%, next is 10, 13, 16 docosatrienoic acid methyl ester Methyl 10, 13, 16-docosatrienoate(19.39%), methyl linolenate (γ-Linolenic acid, methyl ester) (15.87%), 1, 3, 6, 6-tetramethyl-3-(3-methyl-3-methylamino butyl)-piperidyl-2-ketone (1, 3, 6, 6-Tetramethyl-3-(3-methyl-3-methy1aminobutyl)-piperidin-2-one) (13.
35%), cis-11,14 eicosadienoic acid methyl ester cis-11,14-Eicosadien-oic acid, methyl ester
(10.11%), Methyl palmitoleinate (9-Octadecynoic acid, methyl ester) (4.84%) etc.
GC-MS analytic process is adopted to study Semen Lepidii (Semen Descurainiae) fatty oil component in conjunction with Kovats retention index.Find that wherein, the material that content enriches the most is 9 containing more chemical composition in Semen Lepidii (Semen Descurainiae) fatty oil, 12-octadecadienoic methyl, accounts for 21.58%, is secondly 10,13,16 docosatrienoic acid methyl ester (19.39%), methyl linolenate (15.87%) etc.Main based on lipid.
embodiment 2
The material that the present embodiment is used and instrument:
1. experiment material
1.1 medicines and reagent
Semen Descurainiae (being purchased from Zhengzhou City's Chinese Medicinal Materials Markets, through being accredited as the dry seed of crucifer descurainia sophia (l.) webb ex prantl Descurainiasophia (L.) Webb. ex Prantl.) awards qualification by Henan College Of Traditional Chinese Medicine Dong Cheng penetrating judgment; Reagent is domestic analytical pure.
1.2 key instrument
AB204-N type ten thousand/electronic balance (Henan Ai Bote development in science and technology company limited), Q-250A3 pulverizer (Rui Da is international), SB-1000 type rotary evaporator (Shanghai Ai Lang Instrument Ltd.), Bio Mate 3S ultraviolet spectrophotometer (Thermo Fisher Scientific), SK6200H type ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.), DFZ-3 type vacuum drying oven (Shanghai Medical constant-temperature equipment company limited), DRT-ZN type electric jacket (Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.), condensing unit (Longchang, Sichuan Province glass apparatus factory).
The present embodiment Semen Lepidii (Semen Descurainiae) fatty oil is by weighing 500g Semen Lepidii (Semen Descurainiae), through pulverizer, the Semen Lepidii (Semen Descurainiae) after weighing is crushed to 50 orders, Semen Lepidii (Semen Descurainiae) after pulverizing is placed in container, in container, then add the petroleum ether of Semen Lepidii (Semen Descurainiae) weight 8 times, reflux, extract, twice at temperature is 60 DEG C, each reflux, extract, 0.4 hour, after extraction terminates, extracting solution filter paper sucking filtration at a slow speed, uses rotary evaporator decompression and solvent recovery by filtrate, oil sample is revolved and steams to constant weight, namely obtain Semen Lepidii (Semen Descurainiae) fatty oil; By obtained Semen Lepidii (Semen Descurainiae) fatty oil for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.
embodiment 3
The material that the present embodiment is used and instrument:
1. experiment material
1.1 medicines and reagent
Semen Descurainiae (being purchased from Zhengzhou City's Chinese Medicinal Materials Markets, through being accredited as the dry seed of crucifer descurainia sophia (l.) webb ex prantl Descurainiasophia (L.) Webb. ex Prantl.) awards qualification by Henan College Of Traditional Chinese Medicine Dong Cheng penetrating judgment; Reagent is domestic analytical pure.
1.2 key instrument
AB204-N type ten thousand/electronic balance (Henan Ai Bote development in science and technology company limited), Q-250A3 pulverizer (Rui Da is international), SB-1000 type rotary evaporator (Shanghai Ai Lang Instrument Ltd.), Bio Mate 3S ultraviolet spectrophotometer (Thermo Fisher Scientific), SK6200H type ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.), DFZ-3 type vacuum drying oven (Shanghai Medical constant-temperature equipment company limited), DRT-ZN type electric jacket (Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.), condensing unit (Longchang, Sichuan Province glass apparatus factory).
The present embodiment Semen Lepidii (Semen Descurainiae) fatty oil is by weighing 300g Semen Lepidii (Semen Descurainiae), through pulverizer, the Semen Lepidii (Semen Descurainiae) after weighing is crushed to 70 orders, Semen Lepidii (Semen Descurainiae) after pulverizing is placed in container, in container, then add the petroleum ether of Semen Lepidii (Semen Descurainiae) weight 12 times, reflux, extract, twice at temperature is 80 DEG C, each reflux, extract, 0.6 hour, after extraction terminates, extracting solution filter paper sucking filtration at a slow speed, uses rotary evaporator decompression and solvent recovery by filtrate, oil sample is revolved and steams to constant weight, namely obtain Semen Lepidii (Semen Descurainiae) fatty oil; By obtained Semen Lepidii (Semen Descurainiae) fatty oil for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.

Claims (2)

1. the application of Semen Lepidii (Semen Descurainiae) fatty oil in preparation treatment eliminating phlegm and stopping cough suppressing panting calming medicine, is characterized in that: described Semen Lepidii (Semen Descurainiae) fatty oil is for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.
2. the application of Semen Lepidii (Semen Descurainiae) fatty oil according to claim 1 in preparation treatment eliminating phlegm and stopping cough suppressing panting calming medicine, it is characterized in that: described Semen Lepidii (Semen Descurainiae) fatty oil be by weigh after Semen Lepidii (Semen Descurainiae) be crushed to 50-70 order through pulverizer, Semen Lepidii (Semen Descurainiae) after pulverizing is placed in container, then in container, add Semen Lepidii (Semen Descurainiae) weight 8-12 petroleum ether doubly, reflux, extract, twice at temperature is 60-90 DEG C, each reflux, extract, 0.4-0.6 hour, after extraction terminates, extracting solution filter paper sucking filtration, filtrate is used rotary evaporator decompression and solvent recovery, oil sample is revolved and steams to constant weight, namely Semen Lepidii (Semen Descurainiae) fatty oil is obtained, described Semen Lepidii (Semen Descurainiae) fatty oil is for the preparation for the treatment of eliminating phlegm and stopping cough suppressing panting calming medicine.
CN201410349621.0A 2014-07-22 2014-07-22 Application of semen lepidii fatty oil in preparing drugs capable of suppressing cough, dispelling phlegm and calming panting Pending CN104306424A (en)

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Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1663582A (en) * 2004-03-05 2005-09-07 骆春秀 Medicine for treating chronic bronchitis and its preparation method

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CN1663582A (en) * 2004-03-05 2005-09-07 骆春秀 Medicine for treating chronic bronchitis and its preparation method

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Application publication date: 20150128