CN104297461A - Method for predicting yield of cowpea - Google Patents
Method for predicting yield of cowpea Download PDFInfo
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- CN104297461A CN104297461A CN201410526011.3A CN201410526011A CN104297461A CN 104297461 A CN104297461 A CN 104297461A CN 201410526011 A CN201410526011 A CN 201410526011A CN 104297461 A CN104297461 A CN 104297461A
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Abstract
The invention discloses a method for predicting yield of cowpea, belonging to the field of biotechnology. The method comprises the following steps: providing leaves of to-be-detected cowpea plants; obtaining the temperature, actual photosynthetic efficiency Y (II), a photochemistry quenching coefficient qL, non-photochemistry quenching coefficient NPQ, non-accommodative energy dissipation quantum yield Y (NO), a maximum photochemistry quantum yield Fv/Fm, a transpiration rate, air conduction degree of air holes of the leaves, the content of chlorophyll a and flower and pod adscission rate of the leaves; substituting the obtained values into a formula 6553.22+227.73X1+3734.57X2-3428.93X3-4128.39X4-8822.36X5-13995.19X6+6.86X7-29.92X8+56.68X9-11.50X10 so as to calculate a yield predicting value D. Compared with an actually observed yield result, the yield result obtained by the method for predicting yield of cowpea is low in error; the yield of the cowpea can be accurately predicted; the yield of the cowpea can be predicted in a cowpea squaring period; the detection period is shortened.
Description
Technical field
The present invention relates to biological technical field, particularly a kind of method predicting cowpea output.
Background technology
Cowpea is important one of the legume vegetable in summer of China, because its wide adaptability, quality are good and economic benefit stablizes dark liking by producers and consumers.
The method of existing qualification cowpea output can affect the factor of output by stepwise regression analysis, its independent variable parameter selected mostly is the organ weight of the cowpea of picking time, single pod weight of such as picking time, the weight per leaf amount etc. of picking time, such qualification mode needs carry out the picking time of cowpea by the time, makes to identify that the cycle of cowpea output is longer.
Summary of the invention
In order to solve in prior art the problem identifying that the cycle of cowpea output is longer, embodiments provide a kind of method predicting cowpea output.Described technical scheme is as follows:
Embodiments provide a kind of method predicting cowpea output, described method comprises:
The blade of cowpea plant to be detected is provided;
Obtain the temperature of described blade, actual photosynthesis efficiency Y (II), photochemical quenching coefficient qL, non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of non-regulated energy dissipation, maximum Photochemical quantum yield Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content and Flower& pod abscission rate;
Substitute into formula D=6553.22+227.73X
1+ 3734.57X
2-3428.93X
3-4128.39X
4-8822.36X
5-13995.19X
6+ 6.86X
7-29.92X
8+ 56.68X
9-11.50X
10, calculate recovery prediction value D, X in formula
1for the temperature of described blade, X
2for described actual photosynthesis efficiency Y (II), X
3for described photochemical quenching coefficient qL, X
4for described non-photochemical quenching coefficient NPQ, X
5for the quantum yield Y (NO) of described non-regulated energy dissipation, X
6for described maximum Photochemical quantum yield Fv/Fm, X
7for described transpiration rate, X
8for described Stoma of Leaves air guide degree, X
9for described Chlorophyll-a Content, X
10for described Flower& pod abscission rate.
Particularly, the temperature of blade described in chlorophyll fluorescence analysis-e/or determining, described actual photosynthesis efficiency Y (II), described photochemical quenching coefficient qL, described non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of described non-regulated energy dissipation and described maximum Photochemical quantum yield Fv/Fm is adopted.
Further, plant photosynthesis analyzer is adopted to measure described transpiration rate and described Stoma of Leaves air guide degree.
Further, the described blade adopting described plant photosynthesis analyzer to measure measures at bright day gas, and the time choosing described blade is 9:00-11:00 in the morning.
Further, adopt the described blade of described chlorophyll fluorescence analysis-e/or determining identical with the described blade adopting described plant photosynthesis analyzer to measure, and measure within the close time.
Particularly, ultraviolet-visible pectrophotometer is adopted to measure described Chlorophyll-a Content.
Further, the method adopting ultraviolet-visible pectrophotometer to measure described Chlorophyll-a Content comprises: shredded by the blade of described cowpea plant to be detected, get 0.1g and put into 10ml mixed extract, soak under dark surrounds, till described blade becomes white completely, obtain leaching liquor, with described mixed extract in contrast, get described leaching liquor and under 440nm, 645nm and 663nm wavelength, on described ultraviolet-visible pectrophotometer, measure absorbance A value respectively, calculated the content of described chlorophyll a by absorbance A value;
Described mixed extract comprises ethanol, acetone and the water that volume ratio is 4.5:4.5:1.
Particularly, described cowpea plant to be detected is in squaring period, initial bloom stage, full-bloom stage, first pod phase or contains the pod phase.
The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is: the method for prediction cowpea output provided by the invention, by the chlorophyll fluorescence parameters and Flower& pod abscission rate that measure cowpea plant Live leaf, comprehensive assessment is carried out to the output of Cowpea plants, wherein chlorophyll fluorescence parameters can show variable or the constant value of photosynthesis of plant mechanism and photosynthetic physiology situation, reflect inside plants situation, affect plant photosynthesis or Photosynthetic parameter can directly or remote effect to the photosynthate of plant, and finally affect the output of plant, the impact of 10 parameters provided by the invention on plant photosynthesis or Photosynthetic is the most remarkable, thus the output of plant is determined by these 10 parameters, simultaneously, the present invention measures chlorophyll fluorescence parameters and Flower& pod abscission rate does not need smudge cells, also biosome can not be hurt, namely above-mentioned 10 parameters can just be measured to live body cowpea plant, make method simple and efficient, the yield result error of the method that the embodiment of the present invention provides and actual observation is little, can Accurate Prediction cowpea output, and just can to realize the prediction to cowpea output squaring period at cowpea, shorten sense cycle.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly, below embodiment of the present invention is described further in detail.
Embodiment
The embodiment of the present invention provides a kind of method predicting cowpea output, and the method comprises:
The blade of cowpea plant to be detected is provided;
Obtain the temperature of blade, actual photosynthesis efficiency Y (II), photochemical quenching coefficient qL, non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of non-regulated energy dissipation, maximum Photochemical quantum yield Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content and Flower& pod abscission rate.
Substitute into formula D=6553.22+227.73X
1+ 3734.57X
2-3428.93X
3-4128.39X
4-8822.36X
5-13995.19X
6+ 6.86X
7-29.92X
8+ 56.68X
9-11.50X
10, calculate recovery prediction value D.X in formula
1for the temperature of blade, temperature is one of photosynthetic principal element of impact, and the too high or too low for temperature of blade all can produce certain inhibiting effect to photosynthesis of plant; X
2for actual photosynthesis efficiency Y (II), Y (II) represents the originally Photochemical Efficiency that PS II reaction center is actual when there being part to close, Y (II) can react plant leaf blade in illumination, account for the share absorbing luminous energy for the energy of electron transmission, and the intensity of reacting with carbon assimilation is closely related; X
3for photochemical quenching coefficient qL, qL is the ratio that the luminous energy of antenna beam absorption in PS II reaction center transmits for chemical electron, directly related with the process such as electron transmission, photosynthetic oxidation, the electronics of reaction center ratio open in qL low reaction PS II and participation carbon dioxide fixation reduces; X
4for non-photochemical quenching coefficient NPQ, NPQ be higher plant when catching excitation energy surplus, can fall with the thermal dissipation of the mode of NPQ by surplus, thus protection photosynthetic structures destroyed; X
5for the quantum yield Y (NO) of non-regulated energy dissipation, it represents that plant reduces by other forms the energy entering photochemistry approach and accounts for total endergonic ratio, Y (NO) is the important indicator of optical damage, Y (NO) height then shows that the regulation mechanism of photochemistry energy conversion and protectiveness is not enough to the luminous energy of plant absorption to be completely consumed, and now plant may sustain damage; X
6for maximum Photochemical quantum yield Fv/Fm, represent that Photosynthetic is used for the maximal efficiency of chemical reaction the luminous energy absorbed, under non-stress condition, the change of this parameter is minimum, and be not subject to the impact of species and growth conditions, under stress conditions, this parameter obviously declines; X
7for transpiration rate, be that plant scatters and disappears an important way of moisture, can promote the conduction of moisture in plant, accelerate mineral matter transport, time rising, carbon dioxide molecule enters plant by pore, thus has an impact to photosynthetic rate; X
8for Stoma of Leaves air guide degree, pore is the door of steam and carbon dioxide turnover, and it controls photosynthesis and the transpiration of plant simultaneously; X
9for Chlorophyll-a Content, the number of chloroplast of unit leaf area, the chlorophyll content height of unit weight all determine the utilization factor of plant to luminous energy; X
10for Flower& pod abscission rate, the height of Flower& pod abscission rate directly affects the output of cowpea.Every factor that can have influence on plant photosynthesis or Photosynthetic can directly or remote effect to the photosynthate of plant, and finally have influence on the output of plant.
Particularly, plant photosynthesis analyzer is adopted to measure transpiration rate and Stoma of Leaves air guide degree.The model of this plant photosynthesis analyzer is YHZ-3052C, adopt the blade of chlorophyll fluorescence analysis-e/or determining to carry out at bright day gas, and the time choosing blade is 9:00-11:00 in the morning.Meanwhile, the closed circuit photosynthetic rate of plant photosynthesis analyzer mensuration blade, intercellular carbon dioxide mean concentration, blade medial temperature, average temperature of air, average relative humidity and average intensity of illumination is adopted.The mean value of the Blade measuring of random selecting cowpea 6 plant is as the measured value of closed circuit photosynthetic rate, intercellular carbon dioxide mean concentration, blade medial temperature, average temperature of air, average relative humidity and average intensity of illumination.
Particularly, the temperature of chlorophyll fluorescence analysis-e/or determining blade, actual photosynthesis efficiency Y (II), photochemical quenching coefficient qL, non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of non-regulated energy dissipation and maximum Photochemical quantum yield Fv/Fm is adopted.
Further, adopt the blade of chlorophyll fluorescence analysis-e/or determining to measure at bright day gas, and the time choosing blade is 9:00-11:00 in the morning, and from the Live leaf of each community random selecting 6 the cowpea plant Experimental Base.Chlorophyll fluorescence analyser used is that the hyperchannel that German WALZ company produces monitors luminoscope Monitoring-PAM continuously, with the positive middle part of the clip fixed blade of luminoscope passage end, get the temperature of mean value as blade of the Blade measuring of 6 cowpea plant, actual photosynthesis efficiency Y (II), photochemical quenching coefficient qL, non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of non-regulated energy dissipation and the measured value of maximum Photochemical quantum yield Fv/Fm.
Meanwhile, chlorophyll fluorescence analyser is adopted can also to measure the quantum yield Y (NPQ) of maximum fluorescence output Fm ', photochemical quenching coefficient qP, non-photochemical quenching coefficient qN, modulability energy dissipation under the actual fluorescence quantum yield F of the random time of blade, light, dark lower initial fluorescence output Fo and dark lower maximum fluorescence output Fm.
Particularly, the blade adopting plant photosynthesis analyzer to measure is identical with adopting the blade of chlorophyll fluorescence analysis-e/or determining, in similar time, successively adopt plant photosynthesis analyzer and chlorophyll fluorescence analyser to measure to the same area of same blade, the wherein close time is no more than two hours.
Particularly, ultraviolet-visible pectrophotometer is adopted to measure Chlorophyll-a Content.
Further, the method adopting ultraviolet-visible pectrophotometer to measure Chlorophyll-a Content comprises: shredded by the blade of cowpea plant to be detected, get 0.1g and put into 10ml mixed extract, soak under dark surrounds, till blade becomes white completely, obtain leaching liquor, with mixed extract in contrast, get leaching liquor and under 440nm, 645nm and 663nm wavelength, on ultraviolet-visible pectrophotometer, measure absorbance A value respectively, calculated the content of chlorophyll a by absorbance A value; Mixed extract comprises ethanol, acetone and the water that volume ratio is 4.5:4.5:1.Meanwhile, ultraviolet-visible pectrophotometer is adopted to measure content of chlorophyll b, carotenoid content and chlorophyll total amount.The mean value of the Blade measuring of random selecting cowpea 6 plant is as the measured value of content of chlorophyll b, carotenoid content and chlorophyll total amount.
Particularly, cowpea plant to be detected is in squaring period, initial bloom stage, full-bloom stage, first pod phase or contains the pod phase.
Meanwhile, the embodiment of the present invention can adopt plant reflecting spectrograph to carry out the mensuration of plant reflectance spectrum parameter to blade, and other parameters that plant reflectance spectrum parameter is used for relating to the embodiment of the present invention together determine optimal regression equation, and concrete grammar comprises:
The mensuration of blade being carried out to plant reflectance spectrum parameter and chlorophyll fluorescence parameters measure synchronously carries out, CI-710 plant leaf blade spectrometer (wavelength band is 400 nm ~ 1000nm) produced in USA is adopted to measure NDVI (the Normalized Difference Vegetation Index of cowpea blade, normalized differential vegetation index), WBI (Water Band Index, leaf water potential), PRI (Photochemical Reflectance Index, photochemistry reflection index) parameter, its spectral resolution is 1.5nm, its blade measured is identical with adopting the blade of chlorophyll fluorescence analysis-e/or determining, time and the time close (being no more than 2 hours) adopting chlorophyll fluorescence analysis-e/or determining of CI-710 plant leaf blade spectrophotometer.During concrete mensuration, CI-710 plant leaf blade spectrometer faces blade downwards, blade keeps flat, the black pad in blade bottom is held, and ensure that blade to be measured is towards unanimously, measure 6 blades (all identical with the blade that chlorophyll fluorescence analyser is chosen) respectively, get 6 and measure the reflectance spectrum parameter value of mean value as this blade.Wherein, the upgrowth situation of vegetation, throughput rate and other biological physics can be understood by measuring normalized site attenuation NDVI, biochemical characteristics is responsive; Can be understood the water regime in plant by the mensuration measuring leaf water potential WBI, when the flow of water of blade is reduced to critical value, this shows the water deficient of plant, needs to pour water; Can for estimating the efficiency of light energy utilization of blade by measuring photochemistry reflection index PRI, very responsive to the change of the carotenoid (especially xanthein) of live plant, carotenoid can identify the utilization factor of photosynthesis light, can be used for research vegetation productivity and coercive, and the aging of crops.
Wherein, the method obtaining production formula is: to Live leaf by chlorophyll fluorescence analysis-e/or determining F, Fm ', leaf temperature, Y (II), qP, qN, qL, NPQ, Y (NO), Y (NPQ), Fo, Fm, Fv/Fm, NDVI, WBI, PRI, closed circuit photosynthetic rate, transpiration rate, Stoma of Leaves air guide degree, intercellular gas concentration lwevel, blade medial temperature, average temperature of air, average relative humidity, average intensity of illumination, Chlorophyll-a Content, content of chlorophyll b, carotenoid content and chlorophyll total amount, totally 29 parameters, using these 29 parameters as independent variable, using actual per mu yield as dependent variable, filter out 10 independents variable having active effects with dependent variable through successive Regression, these 10 independents variable are respectively: the temperature of blade, Y (II), qL, NPQ, Y (NO), Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content and Flower& pod abscission rate, set up the optimal regression equation of recovery prediction value (D value), concrete steps:
(1) data input: data entry format is behavior sample in DPSV7.05 software, one is classified as a variable, and input 29 independents variable successively, dependent variable per mu yield is placed on rightmost, after a sample has inputted, continue the next sample of input; Wherein, DPSV7.05 software is data handling system (Data Processing System, DPS), and this data handling system can be used for experimental design, statistical study and data mining.
(2) regression equation: on request after form input data, utilize DPSV7.05 software to carry out stepwise regression analysis, according to the size not introducing variable F value, select to reject variable and still introduce variable, introduce after remarkable variable terminates, by " OK ", namely obtain optimal regression equation.
Optimal regression equation is D=6553.22+227.73X
1+ 3734.57X
2-3428.93X
3-4128.39X
4-8822.36X
5-13995.19X
6+ 6.86X
7-29.92X
8+ 56.68X
9-11.50X
10, coefficient R=0.9944 of equation, F value=115.25, P value=0.0001, surplus standard deviation S=42.21, the coefficient R a=0.9901 after adjustment, difference is extremely remarkable, coefficient of determination R
2=0.98885, residue path coefficient=0.10561, as can be seen here, the influential effect of these 10 indexs to yield level distribution variation reaches 98.885%.Simultaneously, the present invention is in order to check the representativeness of these 10 parameters in these 29 parameters and relative importance thereof, on the basis of successive Regression, respectively path analysis is carried out to 10 individual event parameters with recovery prediction value (D value) significant correlation, has utilized correlation matrix to obtain path coefficient.The results of path analysis shows that the relative importance of 10 character pair yield level comprehensive evaluation values is followed successively by: Stoma of Leaves air guide degree > transpiration rate > qL > Y (NO) > Fv/Fm > Y (II) > leaf temperature > Flower& pod abscission rate > NPQ > Chlorophyll-a Content.
The cowpea variety to be detected that the embodiment of the present invention provides is for planting silver-colored wild goose cowpea, and this cowpea plant is seeded in test base, and railway carriage or compartment ridging opened by 1.33m bag ditch, the group arrangement of random district, plot area is 19.95 ㎡, and cave is apart from 0.3m, line-spacing 0.5m, field management is with general land for growing field crops.
When cowpea plant to be detected is in squaring period, measure the temperature of its blade, Y (II), qL, NPQ, Y (NO), Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content, Flower& pod abscission rate be respectively 29.4,0.68,0.79,0.05,0.31,0.015,0.74,901.87mmol/m
2s, 155.78mmol/m
2s, 2.4mg/g, 31.47%, substitute into formula D=6553.22+227.73X
1+ 3734.57X
2-3428.93X
3-4128.39X
4-8822.36X
5-13995.19X
6+ 6.86X
7-29.92X
8+ 56.68X
9-11.50X
10, calculating D value is 1080.18, and cowpea plant to be detected is cultured to maturation and measures its actual output, its actual output is 1114.84Kg/667, and the error of this D value and actual output observed reading is 3.11%.
When cowpea plant to be detected is in initial bloom stage, measure the temperature of its blade, Y (II), qL, NPQ, Y (NO) Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content, Flower& pod abscission rate be respectively 29.4,0.69,0.9,0.02,0.3,0.72,1093.98mmol/m
2s, 199.63mmol/m
2s, 2.16mg/g, 33.15%, substitute into formula D=6553.22+227.73X1+3734.57X2-3428.93X3-4128.39X4-8822.36 X5-13995.19X6+6.86X7-29.92X8+56.68X9-11.50X10, calculating D value is 1204.99, and the error of this D value and actual output observed reading is-2.77%.
When cowpea plant to be detected is in full-bloom stage, measure the temperature of its blade, Y (II), qL, NPQ, Y (NO), Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content, Flower& pod abscission rate be respectively 29.1,0.67,0.69,0.05,0.31,0.76,1117.91mmol/m
2s, 204.18mmol/m
2s, 1.19mg/g, 35.12%, 955.87Kg/667, substitute into formula D=6553.22+227.73X1+3734.57X2-3428.93X3-4128.39X4-8822.36 X5-13995.19X6+6.86X7-29.92X8+56.68X9-11.50X10, calculating D value is 960.52, and the error of this D value and actual output observed reading is-0.49%.
Cowpea plant to be detected be in just the pod phase time, measure the temperature of its blade, Y (II), qL, NPQ, Y (NO), Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content, Flower& pod abscission rate be respectively 28.6,0.67,0.47,0.01,0.33,0.81,1062.63mmol/m
2s, 189.88mmol/m
2s, 2.06mg/g, 32.15%, substitute into formula D=6553.22+227.73X1+3734.57X2-3428.93X3-4128.39X4-8822.36 X5-13995.19X6+6.86X7-29.92X8+56.68X9-11.50X10, calculating D value is 929.20, and the error of this D value and actual output observed reading is-0.86%.
When cowpea plant to be detected is in full-bloom stage, measure the temperature of its blade, Y (II), qL, NPQ, Y (NO), Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content, Flower& pod abscission rate be respectively 29.0,0.72,0.69,0.06,0.27,0.8,967.33mmol/m
2s, 165.48mmol/m
2s, 2.89mg/g, 41.42%, substitute into formula D=6553.22+227.73X1+3734.57X2-3428.93X3-4128.39X4-8822.36 X5-13995.19X6+6.86X7-29.92X8+56.68X9-11.50X10, calculating D value is 1025.35, and the error of this D value and actual output observed reading is 0.22%.
As can be seen here, the method of prediction cowpea output provided by the invention, by the chlorophyll fluorescence parameters and Flower& pod abscission rate that measure cowpea plant Live leaf, comprehensive assessment is carried out to the output of Cowpea plants, wherein chlorophyll fluorescence parameters can show variable or the constant value of photosynthesis of plant mechanism and photosynthetic physiology situation, reflect inside plants situation, affect plant photosynthesis or Photosynthetic parameter can directly or remote effect to the photosynthate of plant, and finally affect the output of plant, the impact of 10 parameters provided by the invention on plant photosynthesis or Photosynthetic is the most remarkable, thus the output of plant is determined by these 10 parameters, simultaneously, the present invention measures chlorophyll fluorescence parameters and Flower& pod abscission rate does not need smudge cells, also biosome can not be hurt, namely above-mentioned 10 parameters can just be measured to live body cowpea plant, make method simple and efficient, the yield result error of the method that the embodiment of the present invention provides and actual observation is little, can Accurate Prediction cowpea output, and just can to realize the prediction to cowpea output squaring period at cowpea, shorten sense cycle.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. predict a method for cowpea output, it is characterized in that, described method comprises:
The blade of cowpea plant to be detected is provided;
Obtain the temperature of described blade, actual photosynthesis efficiency Y (II), photochemical quenching coefficient qL, non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of non-regulated energy dissipation, maximum Photochemical quantum yield Fv/Fm, transpiration rate, Stoma of Leaves air guide degree, Chlorophyll-a Content and Flower& pod abscission rate;
Substitute into formula D=6553.22+227.73X
1+ 3734.57X
2-3428.93X
3-4128.39X
4-8822.36X
5-13995.19X
6+ 6.86X
7-29.92X
8+ 56.68X
9-11.50X
10, calculate recovery prediction value D, X in formula
1for the temperature of described blade, X
2for described actual photosynthesis efficiency Y (II), X
3for described photochemical quenching coefficient qL, X
4for described non-photochemical quenching coefficient NPQ, X
5for the quantum yield Y (NO) of described non-regulated energy dissipation, X
6for described maximum Photochemical quantum yield Fv/Fm, X
7for described transpiration rate, X
8for described Stoma of Leaves air guide degree, X
9for described Chlorophyll-a Content, X
10for described Flower& pod abscission rate.
2. method according to claim 1, it is characterized in that, adopt the temperature of blade described in chlorophyll fluorescence analysis-e/or determining, described actual photosynthesis efficiency Y (II), described photochemical quenching coefficient qL, described non-photochemical quenching coefficient NPQ, the quantum yield Y (NO) of described non-regulated energy dissipation and described maximum Photochemical quantum yield Fv/Fm.
3. method according to claim 2, is characterized in that, adopts plant photosynthesis analyzer to measure described transpiration rate and described Stoma of Leaves air guide degree.
4. method according to claim 3, is characterized in that, the described blade adopting described plant photosynthesis analyzer to measure measures at bright day gas, and the time choosing described blade is 9:00-11:00 in the morning.
5. method according to claim 4, is characterized in that, adopts the described blade of described chlorophyll fluorescence analysis-e/or determining identical with the described blade adopting described plant photosynthesis analyzer to measure, and measures within the close time.
6. method according to claim 1, is characterized in that, adopts ultraviolet-visible pectrophotometer to measure described Chlorophyll-a Content.
7. method according to claim 6, it is characterized in that, the method adopting ultraviolet-visible pectrophotometer to measure described Chlorophyll-a Content comprises: shredded by the blade of described cowpea plant to be detected, get 0.1g and put into 10ml mixed extract, soak under dark surrounds, till described blade becomes white completely, obtain leaching liquor, with described mixed extract in contrast, get described leaching liquor respectively at 440nm, on described ultraviolet-visible pectrophotometer, absorbance A value is measured under 645nm and 663nm wavelength, the content of described chlorophyll a is calculated by absorbance A value,
Described mixed extract comprises ethanol, acetone and the water that volume ratio is 4.5:4.5:1.
8. method according to claim 1, is characterized in that, described cowpea plant to be detected is in squaring period, initial bloom stage, full-bloom stage, first pod phase or contains the pod phase.
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| CN107389548A (en) * | 2017-08-02 | 2017-11-24 | 浙江大学 | The method for early prediction and siliqua of oilseed rape shade of a kind of yield of rape |
| CN109241659A (en) * | 2018-09-27 | 2019-01-18 | 江汉大学 | A method of estimation Fresh edible soybean leaf chlorophyll a/b ratio |
| CN112131948A (en) * | 2020-08-25 | 2020-12-25 | 航天信德智图（北京）科技有限公司 | Actual measurement data-based Guangxi camellia oleifera yield estimation method |
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