CN104256048A - Preparation method of high-load curcumin soybean protein nano product - Google Patents
Preparation method of high-load curcumin soybean protein nano product Download PDFInfo
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Abstract
The invention discloses a preparation method of a high-load curcumin soybean protein nano product, belonging to the technical field of a production process of a functional food. The preparation method comprises the following steps: step 1: adding soybean protein into water; agitating and dispersing at a room temperature; hydrating for 6-12 hours to obtain a soybean protein dispersed solution; step 2: adding curcumin into absolute ethanol and agitating and dissolving to obtain a curcumin ethanol solution; and step 3: adding the curcumin ethanol solution into the soybean protein dispersed solution at a room temperature under an agitating condition; agitating and sufficiently mixing; and centrifuging and drying centrifugal liquid supernatant to obtain powder, namely the high-load curcumin soybean protein nano product. According to the preparation method of the high-load curcumin soybean protein nano product, through utilization of structural properties and thermal pretreatment moderate denaturation of the soybean protein, the soybean protein is urged to be combined with curcumin to form the high-load curcumin soybean protein nano product, and the water solubility and the stability of curcumin can be greatly improved; and the production process is simple and safe and can be applied to food industries.
Description
Technical field
The present invention relates to bioactivator or functional component nano biological goods, and the technical field of producing of functional food, be specifically related to a kind of preparation method of high load curcumin soybean protein nanometer products.
Background technology
Add healthy useful bioactivator in food, if vitamin, probio, active peptide, antioxidant etc. are main paties present stage researching and developing functional food and healthy food.Curcumin has oxidation, anticancer, antibacterial, the bioactivator that regulates the physiological regulation functions such as inflammatory factor as a class, and the application in food, pharmaceuticals has become a kind of trend.Curcumin is mainly derived from the rhizome of Zingiber ginger, and its molecular formula is C
21h
20o
6, belong to polyphenols, dissolve in the organic solvents such as ethanol, acetic acid, acetone and chloroform, water-soluble hardly, the solubility in water is 11ng/mL.Just because the dissolubility of curcumin in water is extremely low, even if it is by human oral, its bioavailability and biological value are also very low, and thus how improving its dispersed and in aqueous phase solubility is solve it to apply restrictive effective technical way.On the other hand, along with the development of nanosecond science and technology, increasing evidence shows many active materials once form the particle of nanoscale, or load is in some specific nano particles, not only can increase the contact area of itself and human body intestinal canal thus improve bioavailability, but also directly penetrate into tissue by fine capillaries absorption principle, thus avoid the removing of liver, and then reach the target more efficiently active component being transported to target site etc.
Soybean protein is a kind of important foodstuffs protein processed from the byproduct of soya-bean oil industry and come, and its abundance, cheap, what is more important, itself just has superior nutrition and physiological regulation function.Soy proteinaceous reduction cholesterol effect has obtained the accreditation of U.S. FDA even.Common commercial soybean protein product mainly contains soybean protein isolate (being called for short SPI) and FSPC.Soybean protein mainly consist of 7S and 11S globulin, both molecular weight are about 180 and 360 kilodaltons respectively.In soybean protein isolate, can there is certain sex change due to the impact by the factor such as Acid-Base and heat treatment in this type of globulin, thus often exist with a kind of nanometer coagulated particles form, and protein surface also can show stronger hydrophobic property.
Up to now, research both at home and abroad finds many food proteins, as lactalbumin and casein, all has the characteristic of some lyophobic dusts of good adsorption, because of but the nanometer delivery vehicles of the potential hydrophobic bioactive material of a class.Some hydrophobic compounds are once form compound with protein, and its dispersiveness in aqueous phase or solubility are just improved greatly, and its bioavailability also can increase to some extent.Based on this, a series of novel bioactive material base-material can be prepared, and the latter can be widely used in various functions the food even research and development of pharmaceuticals.
Summary of the invention
The object of the invention is the weak point applied in food and association area to solve curcumin, providing one can significantly improve curcumin water solubility and heat endurance, prepare the method for high load curcumin soybean protein nanometer products.The key of the method makes curcumin disperse efficiently and be incorporated into soybean protein, and then form the albumen composition with nanoscale.
Object of the present invention realizes especially by following technical proposals:
A preparation method for high load curcumin soybean protein nanometer products, comprises the steps:
(1) first step: soybean protein is added to the water, stirring at normal temperature is disperseed, and aquation 6 ~ 12 hours, obtains soybean protein dispersion liquid;
Preferably, soybean protein with the solid-to-liquid ratio of water is in step (1): correspondingly in every 10 premium on currency add 0.1 ~ 1.0 kilogram of soybean protein;
The method of described dispersed with stirring is mechanical agitation or magnetic agitation;
(2) second step: added by curcumin in absolute ethyl alcohol, stirring and dissolving, obtains curcumin ethanolic solution;
Preferably, in step (2), curcumin with the solid-to-liquid ratio of absolute ethyl alcohol is: every 1 liter of absolute ethyl alcohol is corresponding adds 3 ~ 5 grams of curcumins;
In second step, if any insoluble matter, centrifugally recovery can be removed;
(3) the 3rd steps: in normal temperature under agitation, be (1 ~ 30) by the volume ratio of curcumin ethanolic solution and soybean protein dispersion liquid: 100, curcumin ethanolic solution is added in soybean protein dispersion liquid, carry out again stirring abundant mixing, centrifugal, get centrifuged supernatant and carry out drying, obtain powder and be described high load curcumin soybean protein nanometer products;
Preferably, in step (3), the volume ratio of curcumin ethanolic solution and soybean protein dispersion liquid is (1 ~ 15): 100;
Preferred, in step (3), the volume ratio of curcumin ethanolic solution and soybean protein dispersion liquid is (2 ~ 12): 100;
Preferably, getting centrifuged supernatant described in step (3), to carry out dry drying mode be freeze drying;
Larger difference is there is in the character of soybean protein with the difference in source, for some commercial soybean albumen, its dispersion in aqueous phase and solubility not good, more be conducive to and curcumin compound to make soybean protein, the further scheme of the method for the invention is: soybean protein dispersion liquid first heats, then mixes with curcumin ethanolic solution; The denaturation temperature of its albumen is depended in the selection of heating condition, and preferred heating condition is temperature 75 ~ 95 DEG C, processes 15 ~ 60 minutes.
The high load curcumin soybean protein nanometer products obtained according to said method not only can be directly used in the research and development of powder system functional (health care) food, and can be used as the soybean protein raw material with health effect and make an addition in dairy products, as stirred yoghurt, even also can be used for the Application and Development of protein beverage.
The present invention has following advantage and effect relative to prior art:
(1) the present invention utilizes the architectural characteristic of soybean protein and the sex change of the pre-heat treatment appropriateness to promote the combination of soybean protein and curcumin, forms high load curcumin soybean protein nanometer products, can greatly improve the water-soluble of curcumin and stability.
(2) production technology that the present invention relates to is simple, safety, is suitable for the application of food industry.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.2 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: added by curcumin in absolute ethyl alcohol, stirring and dissolving, is mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(3) the 3rd steps: soybean protein dispersion liquid is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:1, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 2
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.2 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 30 minutes at 75 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: the soybean protein dispersion liquid of second step gained is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:1, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 3
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.2 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 30 minutes at 85 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: the soybean protein dispersion liquid of second step gained is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:1, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 4
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.2 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 30 minutes at 95 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: the soybean protein dispersion liquid of second step gained is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:1, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
The supernatant of the centrifugal gained of embodiment 1 ~ 4 is all faint yellow, clear solution;
Calculate after measured, the soybean protein dispersion liquid that embodiment 1 does not heat is suitable to the load rate of curcumin with the soybean protein dispersion liquid that embodiment 2 ~ 4 heats, and be all greater than 99%, the curcumin capacity value of every gram of soybean protein reaches 0.9 milligram;
The measure and calculation method of load rate is:
The measure and calculation method of capacity value is:
Found by AFM Analysis, the high load curcumin soybean protein nanometer products of embodiment 1 ~ 4 gained exists with spherical nanoparticle form; After the freeze-dried powder of high load curcumin soybean protein nanometer products redissolves, free curcumin is separated out, and solution be faint yellow, transparence, as seen and soybean protein compound can significantly improve the solubility of curcumin in aqueous phase and stability.
Embodiment 5
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.8 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, regulates pH to neutral, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: added by curcumin in absolute ethyl alcohol, stirring and dissolving, is mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(3) the 3rd steps: soybean protein dispersion liquid is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:2, magnetic agitation 12 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products.
Embodiment 6
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.8 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, regulates pH to neutral, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 30 minutes at 75 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: the soybean protein dispersion liquid of second step gained is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:2, magnetic agitation 12 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 7
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.8 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, regulates pH to neutral, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 30 minutes at 85 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: the soybean protein dispersion liquid of second step gained is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:2, magnetic agitation 12 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 8
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.8 kilogram of soybean protein corresponding in every 10 premium on currency, added by soybean protein in distilled water, regulates pH to neutral, normal temperature lower magnetic force stirs 2 hours, then in 4 DEG C of hold over night, make the abundant aquation of soybean protein, obtain soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 30 minutes at 95 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: the soybean protein dispersion liquid of second step gained is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:2, magnetic agitation 12 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
The supernatant of the centrifugal gained of embodiment 5 ~ 8 is all yellow, clear solution;
Calculate after measured, in embodiment 5, the curcumin load rate of soybean protein is 96.82%, and the curcumin capacity value of every gram of soybean protein is 1.09 milligrams; In embodiment 6, the curcumin load rate of soybean protein is 98.12%, and the curcumin capacity value of every gram of soybean protein is 1.10 milligrams; In embodiment 7, the curcumin load rate of soybean protein is 98.54%, and the curcumin capacity value of every gram of soybean protein is 1.11 milligrams; In embodiment 8, the curcumin load rate of soybean protein is 99.09%, and the curcumin capacity value of every gram of soybean protein is 1.12 milligrams.Through contrast, heat pre-treatment can improve the weight bearing power of soybean protein to curcumin.
Found by AFM Analysis, the high load curcumin soybean protein nanometer products of embodiment 5 ~ 8 gained exists with spherical nanoparticle form; After the freeze-dried powder redissolution of the high load curcumin soybean protein nanometer products of each embodiment, the solution color obtained and transparency still with drying before sample suitable, and free curcumin is separated out, visible and soybean protein compound can significantly improve the solubility of curcumin in aqueous phase and stability.
Embodiment 9
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.5 kilogram of soybean protein corresponding in every 10 premium on currency, soybean protein is added in distilled water, regulate pH to neutral, normal temperature lower magnetic force stirs 2 hours, and abundant aquation 8 hours, obtains soybean protein dispersion liquid;
(2) second step: added by curcumin in absolute ethyl alcohol, stirring and dissolving, is mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(3) the 3rd steps: soybean protein dispersion liquid is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:12, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products.
Embodiment 10
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.5 kilogram of soybean protein corresponding in every 10 premium on currency, soybean protein is added in distilled water, regulate pH to neutral, normal temperature lower magnetic force stirs 2 hours, and abundant aquation 8 hours, obtains soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 10 minutes at 75 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: soybean protein dispersion liquid is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:12, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 11
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.5 kilogram of soybean protein corresponding in every 10 premium on currency, soybean protein is added in distilled water, regulate pH to neutral, normal temperature lower magnetic force stirs 2 hours, and abundant aquation 8 hours, obtains soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 10 minutes at 85 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: soybean protein dispersion liquid is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:12, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
Embodiment 12
A preparation method for high load curcumin soybean protein nanometer products, specifically comprises the steps:
(1) first step: by the solid-to-liquid ratio adding 0.5 kilogram of soybean protein corresponding in every 10 premium on currency, soybean protein is added in distilled water, regulate pH to neutral, normal temperature lower magnetic force stirs 2 hours, and abundant aquation 8 hours, obtains soybean protein dispersion liquid;
(2) second step: above-mentioned soybean protein dispersion liquid is heated 10 minutes at 95 DEG C, is cooled to room temperature afterwards;
(3) the 3rd steps: added by curcumin in absolute ethyl alcohol, stirring and dissolving, are mixed with the curcumin ethanolic solution that concentration is 4.5g/L;
(4) the 4th steps: soybean protein dispersion liquid is mixed with curcumin ethanolic solution, the volume ratio of soybean protein dispersion liquid and curcumin ethanolic solution is 100:12, magnetic agitation 6 hours, be separated removing insoluble matter, get centrifuged supernatant and carry out freeze drying, obtain powder and be high load curcumin soybean protein nanometer products;
The supernatant of the centrifugal gained of embodiment 9 ~ 12 is all yellow, clear solution;
In embodiment 9, the curcumin load rate of soybean protein is 79.27%, and the curcumin capacity value of every gram of soybean protein is 8.56 milligrams; In embodiment 10, the curcumin load rate of soybean protein is 80.73%, and the curcumin capacity value of every gram of soybean protein is 8.72 milligrams; In embodiment 11, the curcumin load rate of soybean protein is 82.94%, and the curcumin capacity value of every gram of soybean protein is 8.96 milligrams; In embodiment 12, the curcumin load rate of soybean protein is 85.23%, and the curcumin capacity value of every gram of soybean protein is 9.21 milligrams.Through contrast, heat pre-treatment can improve the weight bearing power of soybean protein to curcumin, and its load rate brings up to 85.23% by 79.27%, and capacity value is increased to every gram of soybean protein 9.21 milligrams of curcumins by every gram of soybean protein 8.56 milligrams of curcumins.
Found by AFM Analysis, the high load curcumin soybean protein nanometer products of embodiment 9 ~ 12 gained exists with spherical nanoparticle form; After the freeze-dried powder of the high load curcumin soybean protein nanometer products of each embodiment redissolves, solution is in yellow, transparence, and free curcumin is separated out, and visible and soybean protein compound can significantly improve the solubility of curcumin in aqueous phase and stability.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (9)
1. a preparation method for high load curcumin soybean protein nanometer products, is characterized in that comprising the steps:
(1) first step: soybean protein is added to the water, stirring at normal temperature is disperseed, and aquation 6 ~ 12 hours, obtains soybean protein dispersion liquid;
(2) second step: added by curcumin in absolute ethyl alcohol, stirring and dissolving, obtains curcumin ethanolic solution;
(3) the 3rd steps: in normal temperature under agitation, be (1 ~ 30) by the volume ratio of curcumin ethanolic solution and soybean protein dispersion liquid: 100, curcumin ethanolic solution is added in soybean protein dispersion liquid, carry out again stirring abundant mixing, centrifugal, get centrifuged supernatant and carry out drying, obtain powder and be described high load curcumin soybean protein nanometer products.
2. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 1, is characterized in that: soybean protein with the solid-to-liquid ratio of water is in step (1): correspondingly in every 10 premium on currency add 0.1 ~ 1.0 kilogram of soybean protein.
3. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 1, is characterized in that: the method for described dispersed with stirring is mechanical agitation or magnetic agitation.
4. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 1, is characterized in that: in step (2), curcumin with the solid-to-liquid ratio of absolute ethyl alcohol is: every 1 liter of absolute ethyl alcohol is corresponding adds 3 ~ 5 grams of curcumins.
5. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 1, is characterized in that: in step (3), the volume ratio of curcumin ethanolic solution and soybean protein dispersion liquid is (1 ~ 15): 100.
6. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 5, is characterized in that: in step (3), the volume ratio of curcumin ethanolic solution and soybean protein dispersion liquid is (2 ~ 12): 100.
7. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 1, is characterized in that: getting centrifuged supernatant described in step (3), to carry out dry drying mode be freeze drying.
8. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 1, is characterized in that: in step (3), soybean protein dispersion liquid first heats, then mixes with curcumin ethanolic solution.
9. the preparation method of a kind of high load curcumin soybean protein nanometer products according to claim 8, is characterized in that: described heating condition is temperature 75 ~ 95 DEG C, processes 15 ~ 60 minutes.
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