CN104250159A - Method for preparing Jishurong nutrient medium - Google Patents

Abstract

The invention relates to a method for preparing a Jishurong nutrient medium, which comprises temperature, humidity and composition proportion as well as process flow. The nutrient medium comprises the following raw materials by proportion: 40-55% of plant straw, 10-20% of pig dung, 5-15% of corn flour, 5-10% of auxiliary material, 10-20% of soybean cake powder and 8-10% of calcium carbonate. The process flow comprises the following steps: 1)fully mixing wheat straw with length of 1-2cm, pig dung, soybean cake powder and corn flour and adding 80-90% of water, 2) establishing a pile by the discharged material and then fermenting for 10-15 days; 3)performing secondary stirring on the fermented material and then adding calcium carbonate with definite proportion, and then simultaneously adding the auxiliary material with definite proportion and then uniformly stirring; 4)packing the discharged material and then disinfecting under high temperature; 5)grounding a disinfected base-material bag for secondary disinfection; and 6)inoculating strain on the processed base-material bag in step 5), and placing at a waste land in woodland at temperature of 18-25 DEG C and under humidity of 85-93% for strain growth.

Description

The fine and soft Nutrient medium preparation method of a kind of Ji Shu

Technical field

The present invention relates to the preparation of a kind of mushroom Nutrient medium, exactly disclose the fine and soft Nutrient medium preparation method of a kind of Ji Shu.

Background technology

Edible mushrooms is desirable whole food or multifunctional food.Eat in the whole world at present and maximum be commonly referred to as mushroom, formal name used at school is Twospore Mushroom.From wild species, screening domestication fine quality production bacterial classification is had great potentialities further.China once tamed also artificial culture success mushroom, auricularia auriculajudae, golden ear, white fungus, straw mushroom, needle mushroom, Hericium erinaceus (Bull. Ex Fr.) Pers., dictyophora phalloidea etc. in the world first, now tame Tricholoma mongolicum Imai, and wild edible fungus King Boletus, morel, the beautiful mushroom of fragrant apricot, rivet mushroom, suillusbovinus, Russula vinosa etc. also can gather in a large number, supply and marketing is in domestic and international market.Artificial breeding technique the further developing also along with science of edible mushrooms, goes on the road of large-scale production gradually.But because edible mushroom is various in style, growing environment differs widely, particularly require comparatively harsh at the fermentation such as soil property of temperature, humidity, illumination and growth, and the key problem in technology of artificial culture is more the preparation of Nutrient medium.

Summary of the invention

The fine and soft Nutrient medium preparation method of a kind of Ji Shu, mainly comprises: 1, temperature, and 2, humidity, 3, Nutrient medium component proportion, 4, technical process; It is characterized in that:

Temperature: 18 DEG C ~ 25 DEG C

Humidity 85% ~ 93%

Nutrient medium component proportion: straw 40% ~ 55%, pig manure 10% ~ 20%, Semen Maydis powder 5% ~ 15%, auxiliary material 5% ~ 10%, soybean cake powder 10% ~ 20%, calcium carbonate 8% ~ 10%;

Technical process: 1. pulverized by straw as long 1 ~ 2cm threadiness, entering stirrer with pig manure, Semen Maydis powder, soybean cake powder fully mixes, and adds the water of 80% ~ 90% in mixing process; 2. the discharging of flow process 1 is built heap fermentation 10 ~ 15 days; 3. the material after fermentation is carried out secondary stirring, and add the calcium carbonate of 8% ~ 10%, add 5% ~ 10% auxiliary material simultaneously, stir; 4. the discharging of flow process 3 is entered dress chartered plane dress bag, be placed in exterminating bacterium in high-temperature boiler; 5. the base-material after sterilizing is wrapped frame and is carried out cultivation indoor secondary exterminating bacterium; 6. pair flow process 5 process after base-material bag inoculation bacterial classification, intersperse among woodland public land 18 DEG C ~ 25 DEG C, humidity 85% ~ 93% bacteria growing.

This technological operation is simple, and raw material sources are extensive, and the Nutrient medium of preparation is nutritious, continues the provisions cycle long, is conducive to growing of mushroom, is applicable to large-scale production and application.

Embodiment

Below in conjunction with specific embodiment, specific features of the present invention is described further.

Embodiment: (1) by straw 41%, pig manure 12%, Semen Maydis powder 12%, auxiliary material 6%, soybean cake powder 19%, calcium carbonate 10% mixes, the water adding 80% ~ 90% spills leaching and builds heap, then overlay film sealing, be exposed to the sun 10 ~ 15 days under daylight (spring and summer season), autumn and winter season should in greenhouse heating fermentation; (2) turn after fully fermenting evenly, pack is placed in exterminating bacterium in high-temperature boiler; (3) in Nutrient medium, bacterial classification is inoculated; (4) keep room temp 18 DEG C ~ 25 DEG C, humidity is 85% ~ 93% bacterium, and woodland public land is planted.

Claims (1)

1. the fine and soft Nutrient medium preparation method of Ji Shu, mainly comprises: 1, temperature, and 2, humidity, 3, Nutrient medium component proportion, 4, technical process; It is characterized in that:

Temperature: 18 DEG C ~ 25 DEG C;

Humidity 85% ~ 93%;

Nutrient medium component proportion: straw 40% ~ 55%, pig manure 10% ~ 20%, Semen Maydis powder 5% ~ 15%, auxiliary material 5% ~ 10%, soybean cake powder 10% ~ 20%, calcium carbonate 8% ~ 10%;

Technical process: 1. pulverized by straw as long 1 ~ 2cm threadiness, entering stirrer with pig manure, Semen Maydis powder, soybean cake powder fully mixes, and adds the water of 80% ~ 90% in mixing process; 2. the discharging of flow process 1 is built heap fermentation 10 ~ 15 days; 3. the material after fermentation is carried out secondary stirring, and add the calcium carbonate of 8% ~ 10%, add 5% ~ 10% auxiliary material simultaneously, stir; 4. the discharging of flow process 3 is entered dress chartered plane dress bag, be placed in exterminating bacterium in high-temperature boiler; 5. the base-material after sterilizing is wrapped frame and is carried out cultivation indoor secondary exterminating bacterium; 6. pair flow process 5 process after base-material bag inoculation bacterial classification, intersperse among woodland public land 18 DEG C ~ 25 DEG C, humidity 85% ~ 93% bacteria growing.