CN104237498A - Preparation method of insulin determination kit employing latex enhanced turbidimetric immunoassay - Google Patents
Preparation method of insulin determination kit employing latex enhanced turbidimetric immunoassay Download PDFInfo
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- CN104237498A CN104237498A CN201310234646.1A CN201310234646A CN104237498A CN 104237498 A CN104237498 A CN 104237498A CN 201310234646 A CN201310234646 A CN 201310234646A CN 104237498 A CN104237498 A CN 104237498A
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- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
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Abstract
A preparation method of an insulin determination kit employing latex enhanced turbidimetric immunoassay is disclosed and relates to the technical field of insulin determination kits. The preparation steps comprise: step 1, preparation of equipment; step 2, pre-production cleaning and detection; step 3, reagent preparation; step 4, reagent subpackaging; and step 5, kit assembling. The kit utilizes an antigen-antibody reaction principle to determine the content of insulin, an antigen-antibody complex is formed through combination by performing specific reaction on insulin in a specimen and corresponding insulin monoclonal antibody latex particles in a reagent, and a certain turbidity is formed, the turbidity is in direct proportion to the insulin content in the specimen when sufficient antibody exist, and by determining the turbidity and comparing with an insulin standard curve, the insulin content of the specimen is obtained, and detection on the insulin content helps to avoid generation of drug resistance caused by excessive usage.
Description
Technical field:
The present invention relates to insulin assay kit technical field, be specifically related to latex enhancing immune turbidimetry insulin assay kit preparation method.
Background technology:
Insulin is a kind of proteohormone secreted as the stimulation of glucose, lactose, ribose, arginine, hyperglycemic factor etc. by endogenous or exogenous material by beta Cell of islet.Insulin uniquely falls hypoglycemic hormone in body, promotes that glycogen, fat, protein synthesize simultaneously.Exogenous insulin is mainly used to treating diabetes, diabetic's early application insulin and superpower antioxidant such as (injection lipoic acid, oral astaxanthin etc.) are expected to the honeymooners occurring the long period, and injection of insulin does not have habituation and dependence.
Little several patient has insulin resistant, and every day, insulin requirement amount was more than 200U, lasted more than 48 hours, and the secondary diabetes person simultaneously caused without ketoacidosis and other endocrine diseases is called insulin resistant.So it is necessary for measuring reagent insulin content.
Summary of the invention:
The object of this invention is to provide latex enhancing immune turbidimetry insulin assay kit preparation method, it utilizes antigen-antibody reaction principle to measure the content of insulin, the insulin monoclonal antibody latex particle specific reaction that insulin in sample is corresponding to reagent, be combined into antigen antibody complex, form certain turbidity, the height of turbidity is directly proportional to the insulin content in sample when sufficient antibodies exists, measure this turbidity, compare with insulin standard curve, insulin content in sample can be drawn, detect insulin content to prevent from using excessive developing immunity to drugs.
In order to solve the problem existing for background technology, the present invention adopts following preparation method: 1, production equipment prepares: mixer: volume is greater than the high-density polyethylene drum 4 that volume produced by reagent.Stirrer: stir the electric mixer being at a slow speed coated with tetrafluoroethene.Weighing equipment: balance is gram 1, precision ± 0.0001.Polyethylene can: pre-title raw material use, has gland bonnet, 2.Raw material weighing spoon, shovel.PH meter, calibration damping fluid, has the pH meter of temperature compensation function and corresponding calibration solution.Instrument, Hitachi 7060 automatic clinical chemistry analyzer.Filter plant, 5 μm, aperture is special.Label: weigh for starting material, indicate material name, weight etc.Label: for indicating: 1-mixer; 2-is product container to be checked by the gross.Signage content: product code, name of product, lot number, date of manufacture, filtration date and operator's signature.
2, produce: prepare before producing: all production equipments must clean.Production line is cleared out a gathering place: production site is only placed this batch package reagent producer goods production operator and dressed protective articles: (a) cleaning takes; B () wears masks; (c) safety goggles; (d) emgloves or disposable glove; (e) hair net.Check various production run list.Measure purified water conductivity used, 2.0 μ s/cm should be less than.Checking of raw material packaging: A, title are correct; B, lot number are for specifying user; C) raw material states of matter is normal.If any not being inconsistent, report to workshop director.Signature, to show that raw material packaging is checked.Pre-title: all raw materials all must claim in advance, and are checked by the second people.Pre-title container has the label of following content: the tare weight of container number, raw material code, lot number, container and lid, raw material net weight, pre-title person signature/date/, checker's signature/date/.Tris 12.114g is taken, insulin monoclonal antibody latex particle 2mg cover for sealed container by the calculated amount of regulation.Check the material quantity taken: general assembly (TW) (raw material+container)=raw material net weight+tare weight.By the data logging on material container on raw material record.
3, product configuration: the preparation of mixer and splash bar; Mixer and splash bar tap water 5 times; Mixer and splash bar use deionized water rinsing 3 times again; It is for subsequent use that drying box dryouies sealing pack; Electric blender is assembled on mixer; Label on the mixing vessel, indicates product code, lot number, title, turnout, date and operator.The purified water of production 90% is added in mixer A, B.In mixer A, add Tris12.114g, be stirred to and dissolve completely.Mend purified water to production 1L.Then the NaOH solution adjust pH to 8.2 (20 DEG C) of 1mol/L is used.With special filter, above-mentioned mixed solution is filtered in another mixer C.In mixer B, add insulin monoclonal antibody latex particle 2mg, be stirred to and dissolve completely.Mend purified water to production 200ml.With special filter, above-mentioned mixed solution is filtered in another mixer D.From mixer C, take out 1 part of 100ml sample, from container D, take out 1 part of 16ml sample, make following process reagent: a, blank absorbency (37 DEG C, 600nm): < 1.2A; B, from mixer sampling carry out the full inspection of semi-manufacture sampling.
4, packing: production environment: a point process of assembling carries out in 100,000 grades of purifying areas, temperature 10 DEG C-26 DEG C, humidity 45%-65%.Clear out a gathering place before packing, get semi-manufacture reagent qualified after testing, check the reagent lot got and whether cause with plan is single, check that whether reagent outward appearance is normal, as reagent becomes turbid, to precipitate or color change should stop packing, and be reported to Production Supervisor.Get material: get according to the production schedule and require the reagent bottle of model, supporting bottle cap, label, prepare package tools, equipment.Divide process of assembling: according to the packing of packing type; Weighing bottle weight: quantity prepares bottle according to plan, weighs the weight of every ten reagent bottles, requires that the bottle weight difference being used for the same lot number reagent of packing is no more than 0.5g, pendulum bottle: undertaken by " packing job instruction " requirement.Packing: use peristaltic pump to carry out packing, note preventing cross pollution, and loading amount is not less than sign loading amount in process.Carry out inspection lid effect by bottle, if any leakage, must not subsequent processing be entered.Label: will check and pass through the label examined and be attached on reagent bottle, by " packing job instruction " operation.Check: check sub-bottling number, reagent loading amount, leakage situation, labeling situation, record and fill in situation.Clear out a gathering place: by " management system of clearing out a gathering place ", cleared out a gathering place in production scene, and fill in record.
5, assemble: neck material: get the material such as instructions, carton, seal, conformity certification needed for assembling insulin double reagent according to the production schedule, carton should first be rolled well shaping before assembling.Semi-finished product inspection: by semi-manufacture by bottle inspection, performs " packing half becomes inspection and guide book ", is responsible for by QA.Assembling: perform " assembling operation guiding book ".By the kit close the cover installed, stick seal, conformity certification, the position of subsides just will pasted, left and right, up and down decile distance.After having assembled, QA carries out finished product sampling, and 1 box got by each lot number finished product reagent, first carries out the inspection of packaging quality, is carried out the inspection of inherent quality again by QC, performs " product finally let pass inspection specification ".Clear out a gathering place by " management system of clearing out a gathering place ", and fill in record of production.Carry out semi-manufacture, the entering of finished product, outbound formality.As semi-manufacture can not the outbound of assembling in time, the storage of semi-manufacture shelf appeal should be pressed.
Principle of work of the present invention: the know-why that this kit adopts is the content utilizing antigen-antibody reaction principle to measure insulin.The insulin monoclonal antibody latex particle specific reaction that insulin in sample is corresponding to reagent, be combined into antigen antibody complex, form certain turbidity, the height of turbidity is directly proportional to the insulin content in sample when sufficient antibodies exists, measure this turbidity, compare with insulin standard curve, insulin content in sample can be drawn.The ratio linearly depending primarily on antibody activity, reaction system and antibody antigen three aspects during immunoturbidimetry measures between antigen concentration and absorbance, so the research of reaction system comprises applied sample amount, the extension rate of sample and reagent, in the reaction time, measure wavelength etc. reaction conditions
The present invention has following beneficial effect: it utilizes antigen-antibody reaction principle to measure the content of insulin, the insulin monoclonal antibody latex particle specific reaction that insulin in sample is corresponding to reagent, be combined into antigen antibody complex, form certain turbidity, the height of turbidity is directly proportional to the insulin content in sample when sufficient antibodies exists, measure this turbidity, compare with insulin standard curve, insulin content in sample can be drawn, detect insulin content and prevent from using excessive developing immunity to drugs.
Embodiment:
This embodiment is by the following technical solutions: it comprises 1, production equipment prepares: mixer: volume is greater than the high-density polyethylene drum 4 that volume produced by reagent.Stirrer: stir the electric mixer being at a slow speed coated with tetrafluoroethene.Weighing equipment: balance is gram 1, precision ± 0.0001.Polyethylene can: pre-title raw material use, has gland bonnet, 2.Raw material weighing spoon, shovel.PH meter, calibration damping fluid, has the pH meter of temperature compensation function and corresponding calibration solution.Instrument, Hitachi 7060 automatic clinical chemistry analyzer.Filter plant, 5 μm, aperture is special.Label: weigh for starting material, indicate material name, weight etc.Label: for indicating: 1-mixer; 2-is product container to be checked by the gross.Signage content: product code, name of product, lot number, date of manufacture, filtration date and operator's signature.
2, produce: prepare before producing: all production equipments must clean.Production line is cleared out a gathering place: production site is only placed this batch package reagent producer goods production operator and dressed protective articles: (a) cleaning takes; B () wears masks; (c) safety goggles; (d) emgloves or disposable glove; (e) hair net.Check various production run list.Measure purified water conductivity used, 2.0 μ s/cm should be less than.Checking of raw material packaging: A, title are correct; B, lot number are for specifying user; C) raw material states of matter is normal.If any not being inconsistent, report to workshop director.Signature, to show that raw material packaging is checked.Pre-title: all raw materials all must claim in advance, and are checked by the second people.Pre-title container has the label of following content: the tare weight of container number, raw material code, lot number, container and lid, raw material net weight, pre-title person signature/date/, checker's signature/date/.Tris 12.114g is taken, insulin monoclonal antibody latex particle 2mg cover for sealed container by the calculated amount of regulation.Check the material quantity taken: general assembly (TW) (raw material+container)=raw material net weight+tare weight.By the data logging on material container on raw material record.
3, product configuration: the preparation of mixer and splash bar; Mixer and splash bar tap water 5 times; Mixer and splash bar use deionized water rinsing 3 times again; It is for subsequent use that drying box dryouies sealing pack; Electric blender is assembled on mixer; Label on the mixing vessel, indicates product code, lot number, title, turnout, date and operator.The purified water of production 90% is added in mixer A, B.In mixer A, add Tris12.114g, be stirred to and dissolve completely.Mend purified water to production 1L.Then the NaOH solution adjust pH to 8.2 (20 DEG C) of 1mol/L is used.With special filter, above-mentioned mixed solution is filtered in another mixer C.In mixer B, add insulin monoclonal antibody latex particle 2mg, be stirred to and dissolve completely.Mend purified water to production 200ml.With special filter, above-mentioned mixed solution is filtered in another mixer D.From mixer C, take out 1 part of 100ml sample, from container D, take out 1 part of 16ml sample, make following process reagent: a, blank absorbency (37 DEG C, 600nm): < 1.2A; B, from mixer sampling carry out the full inspection of semi-manufacture sampling.
4, packing: production environment: a point process of assembling carries out in 100,000 grades of purifying areas, temperature 10 DEG C-26 DEG C, humidity 45%-65%.Clear out a gathering place before packing, get semi-manufacture reagent qualified after testing, check the reagent lot got and whether cause with plan is single, check that whether reagent outward appearance is normal, as reagent becomes turbid, to precipitate or color change should stop packing, and be reported to Production Supervisor.Get material: get according to the production schedule and require the reagent bottle of model, supporting bottle cap, label, prepare package tools, equipment.Divide process of assembling: according to the packing of packing type; Weighing bottle weight: quantity prepares bottle according to plan, weighs the weight of every ten reagent bottles, requires that the bottle weight difference being used for the same lot number reagent of packing is no more than 0.5g, pendulum bottle: undertaken by " packing job instruction " requirement.Packing: use peristaltic pump to carry out packing, note preventing cross pollution, and loading amount is not less than sign loading amount in process.Carry out inspection lid effect by bottle, if any leakage, must not subsequent processing be entered.Label: will check and pass through the label examined and be attached on reagent bottle, by " packing job instruction " operation.Check: check sub-bottling number, reagent loading amount, leakage situation, labeling situation, record and fill in situation.Clear out a gathering place: by " management system of clearing out a gathering place ", cleared out a gathering place in production scene, and fill in record.
5, assemble: neck material: get the material such as instructions, carton, seal, conformity certification needed for assembling insulin double reagent according to the production schedule, carton should first be rolled well shaping before assembling.Semi-finished product inspection: by semi-manufacture by bottle inspection, performs " packing half becomes inspection and guide book ", is responsible for by QA.Assembling: perform " assembling operation guiding book ".By the kit close the cover installed, stick seal, conformity certification, the position of subsides just will pasted, left and right, up and down decile distance.After having assembled, QA carries out finished product sampling, and 1 box got by each lot number finished product reagent, first carries out the inspection of packaging quality, is carried out the inspection of inherent quality again by QC, performs " product finally let pass inspection specification ".Clear out a gathering place by " management system of clearing out a gathering place ", and fill in record of production.Carry out semi-manufacture, the entering of finished product, outbound formality.As semi-manufacture can not the outbound of assembling in time, the storage of semi-manufacture shelf appeal should be pressed
Principle of work of the present invention: the know-why that this kit adopts is the content utilizing antigen-antibody reaction principle to measure insulin.The insulin monoclonal antibody latex particle specific reaction that insulin in sample is corresponding to reagent, be combined into antigen antibody complex, form certain turbidity, the height of turbidity is directly proportional to the insulin content in sample when sufficient antibodies exists, measure this turbidity, compare with insulin standard curve, insulin content in sample can be drawn.The ratio linearly depending primarily on antibody activity, reaction system and antibody antigen three aspects during immunoturbidimetry measures between antigen concentration and absorbance, so the research of reaction system comprises applied sample amount, the extension rate of sample and reagent, in the reaction time, measure wavelength etc. reaction conditions
Embodiment:
1.1 sample requirements
Should be separated in time after serum blood sampling, not haemolysis serum.Sample 2-8 DEG C Absorbable organic halogens 7 days ,-20 DEG C of Absorbable organic halogens one month (freezing sample redissolves once only, can not repeatedly freeze-thaw)
1.2 wavelength
Latex enhancing immune turbidimetry principle is light scattering by deflection of light during immune turbidity reagent, thus transmitted light is weakened.This just determines predominant wavelength and absorbance is inverse ratio, so the selection of wavelength determines the size of sensitivity.
Insulin assay kit detects data under 600nm wavelength and Kang Te Insulin Kit correlativity is the highest, and automatic clinical chemistry analyzer can conveniently detect, therefore selects 600nm to be the test wavelength of this product.
1.3 sample reagent ratios
The concentration of potent antibodies in the number decision reaction volume of amount of reagent, and sample reagent is than the range of linearity that can affect reagent.
Conclusion: known by above test figure, when blood serum sample application of sample amount is 8 microlitre, linear case is best.
1.4 the reaction time
The insulin agent reaction time is 4 minutes, reads the absorbance changing value Δ A that R2 adds rear 0min-4min, calculates sample results with Δ A value.
Insulin agent, also according to the testing response time of Kang Te reagent, observes the result that insulin agent reacts 4 minutes.
The absorbance changing value Δ A fixing 2 points (namely R2 adds rear 0min-4min) according to reading carries out result calculating, therefore selects within 4 minutes, to be the reaction time.
1.5 results calculate
Two-point calibration, adopts linear computation schema, as process such as LINEAR, can try to achieve INS content to measure pipe Δ A.
1.6 reference values (term of reference)
Normal reference value 3.0-23.0uIU/mL.
1.7 calibration stepss: suggestion uses insulin calibration object often to criticize and calibrates product.
1.8 method of quality control:
Often criticize and all should place quality-control product and tested sample is tested simultaneously, need re-start when Quality Control result exceeds standard and confirm to detect.
Arrange by above parameter reference document claims, proved by performance test, this parameter can ensure that product test performance is normal.
Claims (1)
1. latex enhancing immune turbidimetry insulin assay kit preparation method, is characterized in that its preparation method: 1, production equipment prepares: mixer: volume is greater than the high-density polyethylene drum 4 that volume produced by reagent.Stirrer: stir the electric mixer being at a slow speed coated with tetrafluoroethene.Weighing equipment: balance is gram 1, precision ± 0.0001.Polyethylene can: pre-title raw material use, has gland bonnet, 2.Raw material weighing spoon, shovel.PH meter, calibration damping fluid, has the pH meter of temperature compensation function and corresponding calibration solution.Instrument, Hitachi 7060 automatic clinical chemistry analyzer.Filter plant, 5 μm, aperture is special.Label: weigh for starting material, indicate material name, weight etc.Label: for indicating: 1-mixer; 2-is product container to be checked by the gross.Signage content: product code, name of product, lot number, date of manufacture, filtration date and operator's signature.2, produce: prepare before producing: all production equipments must clean.Production line is cleared out a gathering place: production site is only placed this batch package reagent producer goods production operator and dressed protective articles: (a) cleaning takes; B () wears masks; (c) safety goggles; (d) emgloves or disposable glove; (e) hair net.Check various production run list.Measure purified water conductivity used, 2.0 μ s/cm should be less than.Checking of raw material packaging: A, title are correct; B, lot number are for specifying user; C) raw material states of matter is normal.If any not being inconsistent, report to workshop director.Signature, to show that raw material packaging is checked.Pre-title: all raw materials all must claim in advance, and are checked by the second people.Pre-title container has the label of following content: the tare weight of container number, raw material code, lot number, container and lid, raw material net weight, pre-title person signature/date/, checker's signature/date/.Tris 12.114g is taken, insulin monoclonal antibody latex particle 2mg cover for sealed container by the calculated amount of regulation.Check the material quantity taken: general assembly (TW) (raw material+container)=raw material net weight+tare weight.By the data logging on material container on raw material record.3, product configuration: the preparation of mixer and splash bar; Mixer and splash bar tap water 5 times; Mixer and splash bar use deionized water rinsing 3 times again; It is for subsequent use that drying box dryouies sealing pack; Electric blender is assembled on mixer; Label on the mixing vessel, indicates product code, lot number, title, turnout, date and operator.The purified water of production 90% is added in mixer A, B.In mixer A, add Tris 12.114g, be stirred to and dissolve completely.Mend purified water to production 1L.Then the NaOH solution adjust pH to 8.2 (20 DEG C) of 1mol/L is used.With special filter, above-mentioned mixed solution is filtered in another mixer C.In mixer B, add insulin monoclonal antibody latex particle 2mg, be stirred to and dissolve completely.Mend purified water to production 200ml.With special filter, above-mentioned mixed solution is filtered in another mixer D.From mixer C, take out 1 part of 100ml sample, from container D, take out 1 part of 16ml sample, make following process reagent: a, blank absorbency (37 DEG C, 600nm): < 1.2A; B, from mixer sampling carry out the full inspection of semi-manufacture sampling.4, packing: production environment: a point process of assembling carries out in 100,000 grades of purifying areas, temperature 10 DEG C-26 DEG C, humidity 45%-65%.Clear out a gathering place before packing, get semi-manufacture reagent qualified after testing, check the reagent lot got and whether cause with plan is single, check that whether reagent outward appearance is normal, as reagent becomes turbid, to precipitate or color change should stop packing, and be reported to Production Supervisor.Get material: get according to the production schedule and require the reagent bottle of model, supporting bottle cap, label, prepare package tools, equipment.Divide process of assembling: according to the packing of packing type; Weighing bottle weight: quantity prepares bottle according to plan, weighs the weight of every ten reagent bottles, requires that the bottle weight difference being used for the same lot number reagent of packing is no more than 0.5g, pendulum bottle: undertaken by " packing job instruction " requirement.Packing: use peristaltic pump to carry out packing, note preventing cross pollution, and loading amount is not less than sign loading amount in process.Carry out inspection lid effect by bottle, if any leakage, must not subsequent processing be entered.Label: will check and pass through the label examined and be attached on reagent bottle, by " packing job instruction " operation.Check: check sub-bottling number, reagent loading amount, leakage situation, labeling situation, record and fill in situation.Clear out a gathering place: by " management system of clearing out a gathering place ", cleared out a gathering place in production scene, and fill in record.5, assemble: neck material: get the material such as instructions, carton, seal, conformity certification needed for assembling insulin double reagent according to the production schedule, carton should first be rolled well shaping before assembling.Semi-finished product inspection: by semi-manufacture by bottle inspection, performs " packing half becomes inspection and guide book ", is responsible for by QA.Assembling: perform " assembling operation guiding book ".By the kit close the cover installed, stick seal, conformity certification, the position of subsides just will pasted, left and right, up and down decile distance.After having assembled, QA carries out finished product sampling, and 1 box got by each lot number finished product reagent, first carries out the inspection of packaging quality, is carried out the inspection of inherent quality again by QC, performs " product finally let pass inspection specification ".Clear out a gathering place by " management system of clearing out a gathering place ", and fill in record of production.Carry out semi-manufacture, the entering of finished product, outbound formality.As semi-manufacture can not the outbound of assembling in time, the storage of semi-manufacture shelf appeal should be pressed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310234646.1A CN104237498A (en) | 2013-06-13 | 2013-06-13 | Preparation method of insulin determination kit employing latex enhanced turbidimetric immunoassay |
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| CN201310234646.1A CN104237498A (en) | 2013-06-13 | 2013-06-13 | Preparation method of insulin determination kit employing latex enhanced turbidimetric immunoassay |
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| CN104237498A true CN104237498A (en) | 2014-12-24 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053364A (en) * | 2016-05-26 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for measuring insulin and preparation method of kit |
| CN106483302A (en) * | 2016-09-29 | 2017-03-08 | 浙江达美生物技术有限公司 | A kind of mensure reagent of insulin and preparation method thereof |
| CN106918708A (en) * | 2015-12-28 | 2017-07-04 | 北京九强生物技术股份有限公司 | A kind of competition law turbid kit of latex enhancing immune transmittance for detecting insulin |
| CN107596933A (en) * | 2017-11-03 | 2018-01-19 | 厦门琥珀日化科技股份有限公司 | A kind of compounding and production process of essence |
-
2013
- 2013-06-13 CN CN201310234646.1A patent/CN104237498A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918708A (en) * | 2015-12-28 | 2017-07-04 | 北京九强生物技术股份有限公司 | A kind of competition law turbid kit of latex enhancing immune transmittance for detecting insulin |
| CN106053364A (en) * | 2016-05-26 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for measuring insulin and preparation method of kit |
| CN106483302A (en) * | 2016-09-29 | 2017-03-08 | 浙江达美生物技术有限公司 | A kind of mensure reagent of insulin and preparation method thereof |
| CN107596933A (en) * | 2017-11-03 | 2018-01-19 | 厦门琥珀日化科技股份有限公司 | A kind of compounding and production process of essence |
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Application publication date: 20141224 |