CN104230472A

CN104230472A - Method for preparing seafood mushroom nutrient medium

Info

Publication number: CN104230472A
Application number: CN201310235538.6A
Authority: CN
Inventors: 不公告发明人
Original assignee: Jiangsu Hongxin Rises Edible Mushroom Co Ltd
Current assignee: Jiangsu Hongxin Rises Edible Mushroom Co Ltd
Priority date: 2013-06-15
Filing date: 2013-06-15
Publication date: 2014-12-24

Abstract

The invention relates to a method for preparing a seafood mushroom nutrient medium, which comprises temperature, humidity, composition proportion as well as process flow. The nutrient medium comprises the following raw materials by proportion: 40-60% of straw, 3-5% of cow dung, 5-15% of quicklime, 23-30% of wood chip, 13-20% of cottonseed, 10-15% of rice bran and 3-10% of auxiliary material. The process flow comprises the following steps: 1)uniformly mixing straw, wood chip, quicklime, cottonseed, cow dung, rice bran and auxiliary material, adding 80-90% of water and building a pile, and coating a film and sealing, exposing for 10-15 days under daylight (in spring and summer seasons), heating and fermenting under room temperature in autumn and winter season; 2)uniformly mixing after fully fermenting, packaging and placing in a high temperature boiler for disinfection; 3) grounding and filling in bed, sealing door and window, disinfecting for second time to a culture chamber; 4)inoculating strain on the nutrient medium; and 5)keeping indoor temperature at 18 DEG C, and growing the strain under condition of humidity at 90-95%.

Description

Seafood mushroom Nutrient medium preparation method

Technical field

The present invention relates to a kind of preparation of mushroom Nutrient medium, exactly disclose a kind of seafood mushroom Nutrient medium preparation method.

Background technology

Edible mushrooms is desirable whole food or multifunctional food.Eat in the whole world at present and maximum be commonly referred to as mushroom, formal name used at school is Twospore Mushroom.From wild species, screening domestication fine quality production bacterial classification is had great potentialities further.China once tamed also artificial culture success mushroom, auricularia auriculajudae, golden ear, white fungus, straw mushroom, needle mushroom, Hericium erinaceus (Bull. Ex Fr.) Pers., dictyophora phalloidea etc. in the world first, now tame Tricholoma mongolicum Imai, and wild edible fungus King Boletus, morel, the beautiful mushroom of fragrant apricot, rivet mushroom, suillusbovinus, Russula vinosa etc. also can gather in a large number, supply and marketing is in domestic and international market.Artificial breeding technique the further developing also along with science of edible mushrooms, goes on the road of large-scale production gradually.But because edible mushroom is various in style, growing environment differs widely, particularly require comparatively harsh at the fermentation such as soil property of temperature, humidity, illumination and growth, and the key problem in technology of artificial culture is more the preparation of Nutrient medium.

Summary of the invention

Seafood mushroom Nutrient medium preparation method, mainly comprises: 1, temperature, and 2, humidity, 3, Nutrient medium component proportion, 4, technical process;

It is characterized in that:

Temperature: 15 DEG C ~ 20 DEG C;

Humidity: 90% ~ 95%;

Nutrient medium component proportion: straw 40% ~ 60%, cow dung 3% ~ 5%, unslaked lime 5% ~ 15%, wood chip 23% ~ 30%, Cotton Seed 13% ~ 20%, rice bran 10% ~ 15%, auxiliary material 3% ~ 10%;

Technical process: 1. straw is pulverized as long 1 ~ 2cm threadiness, enter stirrer with the Cotton Seed after pulverizing and wood chip and fully mix, add the water of 80% ~ 90% in mixing process; 2. the discharging of flow process 1 is built heap fermentation 10 ~ 15 days; 3. the material after fermentation is carried out secondary stirring, and add the unslaked lime of 5% ~ 15%, add cow dung, rice bran and auxiliary material in proportion simultaneously, stir; 4. the discharging of flow process 3 is entered dress chartered plane dress bag, be placed in exterminating bacterium in high-temperature boiler; 5. the base-material after sterilizing is wrapped frame and is carried out cultivation indoor secondary exterminating bacterium; 6. the base-material bag inoculation bacterial classification after pair flow process 5 process, keeps room temp 15 DEG C ~ 20 DEG C, humidity 90% ~ 95% bacteria growing.

This technological operation is simple, and raw material sources are extensive, and the Nutrient medium of preparation is nutritious, continues the provisions cycle long, is conducive to growing of mushroom, is applicable to large-scale production and application.

Embodiment

Below in conjunction with specific embodiment, technical characteristic of the present invention is described further.

Embodiment: straw 40%, wood chip 23%, unslaked lime 5%, Cotton Seed 13%, cow dung 3%, rice bran 10%, auxiliary material 3% mix by (1), the water adding 80% ~ 90% spills leaching and builds heap, then overlay film sealing, be exposed to the sun 10 ~ 15 days under daylight (spring and summer season), autumn and winter season should in greenhouse heating fermentation; (2) turn after fully fermenting evenly, pack is placed in exterminating bacterium in high-temperature boiler; (3) door and window closed by added bed of filling out, and carries out secondary exterminating bacterium to culturing chamber; (4) in Nutrient medium, bacterial classification is inoculated; (5) keep room temp 18 DEG C, humidity is at 90% ~ 95% bacteria growing.

Claims

1. seafood mushroom Nutrient medium preparation method, mainly comprises: temperature, humidity, component proportion and technical process;

It is characterized in that:

Temperature: 15 DEG C ~ 20 DEG C;

Humidity: 90% ~ 95%;

Component proportion: straw 40% ~ 60%, cow dung 3% ~ 5%, unslaked lime 5% ~ 15%, wood chip 23% ~ 30%, Cotton Seed 13% ~ 20%, rice bran 10% ~ 15%, auxiliary material 3% ~ 10%;

Technical process: (1). straw is pulverized for long 1 ~ 2cm threadiness, enter stirrer with the Cotton Seed after pulverizing and wood chip and fully mix, in mixing process, add the water of 80% ~ 90%; (2). the discharging of flow process 1 is built heap fermentation 10 ~ 15 days; (3). the material after fermentation is carried out secondary stirring, and adds the unslaked lime of 5% ~ 15%, add cow dung, rice bran and auxiliary material in proportion simultaneously, stir; (4). the discharging of flow process 3 is entered dress chartered plane dress bag, be placed in exterminating bacterium in high-temperature boiler; (5). the base-material after sterilizing is wrapped frame and is carried out cultivation indoor secondary exterminating bacterium; (6). the base-material bag inoculation bacterial classification after processing flow process 5, keeps room temp 15 DEG C ~ 20 DEG C, humidity 90% ~ 95% bacteria growing.