CN104225627A - Function and application of white cell immune globulin toll-like receptor subfamily B member 4 in treatment of atherosclerosis - Google Patents

Abstract

The invention discloses a function and application of a white cell immune globulin toll-like receptor subfamily B member 4 in treatment of the atherosclerosis and belongs to the field of a function and application of a gene. According to the invention, an ApoE<-/-> mouse and an LILRB4<-/->ApoE<-/-> mouse are used as experimental objects and an atherosclerosis module is obtained by high fat diet induction; the result shows that compared with the ApoE<-/-> mouse, the gene defect of the LILRB4 obviously increases the plaque area of an aorta, reduces stability of an aortic sinus plaque and obviously deteriorates the inflammatory response. The invention shows that the function of the LILRB4 in the atherosclerosis mainly represents that the LILRB4 has an effect of inhibiting the aortic plaque from being formed and particularly has an effect of inhibiting the atherosclerosis. Aiming at the function of the LILRB4, the LILRB4 can be used for preparing a medicament for preventing, relieving and/or treating the atherosclerosis.

Description

The function and application of leukocytic immunity globulin sample receptor subfamily B member 4 in treatment atherosclerosis

 

Technical field

The invention belongs to function and the application of gene, be specifically related to a kind of leukocytic immunity globulin sample receptor subfamily B member 4(LILRB4) treating the function and application in atherosclerosis, specifically LILRB4 prevents in preparation, alleviates and/or treat in atherosclerotic medicine and apply.

Background technology

Cardiovascular and cerebrovascular disease be main lethal in many developed countries because of, also raise year by year at the sickness rate of China and fatality rate.The basis of cardiovascular and cerebrovascular disease is atherosclerosis (Atheosclersisis, AS), and atherosclerosis can make ductus arteriosus wall thicken, hardening, luminal stenosis, causes a lot of cardiocerebrovasculaevents events to occur.And the coronary artery acute stenosis that causes of the breaking of atherosclerosis unstable spot, hematoblastic gathering and thrombosis and obturation are the major reasons causing acute coronary syndrome (acute coronary syndrome, ACS).

Atherosclerosis is multiple gene, a kind of chronic inflammation disease that risk factor and immunologic mechanism participate in jointly, the inflammatory and immune response of local and whole body plays an important role in atherosclerosis generation evolution, inherent immunity and adaptive immunity participate in regulating atherosclerotic lesion jointly, pathology show as large, medium-sized artery many places Mottling formation, be apt to occur in blood shunt, the regions such as the bending and arterial branch of tremulous pulse, its characteristics of lesion is that in blood, lipid deposits at endarterium, cause inner membrance stove fibrous thickening, focus deep is the medicated porridge sample material formed by slough and extracellular lipid pond.Panimmunity cell is there is in atheromatous plaque, wherein with macrophage and T cell the most common, in addition a small amount of dendritic cell (Dentritic cell is also had, DC), natural killer cell (natural killer cell, NK cell) and mastocyte (mast cell) etc., occasionally there is bone-marrow-derived lymphocyte.

The macroscopic damage of atherosclerosis earliest period is fatty streaks, forms primarily of the Macrophage derived foamy cell that intake of a large amount of cholesterol.Mononuclear cell in blood circulation is attached to reactive endothelial cells at tremulous pulse damageable zone, starts the formation of fatty streaks, under the attraction that the mononuclear cell sticked is subject to the chemistry of local generation to ingratiate with molecule subsequently moves to inner membrance, and is divided into macrophage further.A large amount of cholesterol ester, in macrophage inner accumulated, forms foam cell, and this is the early stage characteristic pathological physiological process of atherogenesis.Atherosclerotic is multifactor coefficient result, and the atherosclerosis risk sexual factor found at present is a lot, but related pins to treatment and control effects all undesirable, blood fat reducing and anti-inflammatory treatment are current topmost remedy measures.

Immunoglobulin superfamily (IgSF) receptor is the important receptor of a class of Macrophage Surface, take part in the immune disease of multiple macrophage induction, and plays a significant role in graft-rejection.IgSF receptor can conduct inhibition or excitatory signal, makes the function of macrophage raise or lower, thus keeps immune homeostasis and produce effective immunne response.Cell immunoglobulin sample receptor (LILR) belongs to IgSF family, can be combined with major histocompatibility complex (MHC) I class or correlation molecule, is expressed in lymphocyte and medullary cell film surface.LILR contains 5 Inhibitory receptor (LILRB1-LILRB5), 3 excitatory receptors (LILRA1-LILRA3), and these receptors have 2-4 IgSF domain outside born of the same parents, and tyrosine residue is contained in cytoplasmic region, can phosphorylation play biological action.LILRB4, also referred to as ILT3, LIR-5, CD85k, is encoded by chromosome 19q 13.4.Existing research confirms that LILRB4 participates in immunologic tolerance (Hao Cheng, Fiyaz Mohammed, Gol Nam, et al. Crystal Structure of Leukocyte Ig-like Receptor LILRB4 (ILT3/LIR-5/

CD85k)?A?MYELOID?INHIBITORY?RECEPTOR?INVOLVED?IN?IMMUNE?TOLERANCE.?J?Biol?Chem.?2011;?286(20):?18013–18025.）。Based on above-mentioned Research foundation, we think that LILRB4 may play key player in the characteristic pathological physiological process of atherogenesis, but its biological function in the characteristic pathological physiological process of atherogenesis and possible mechanism of action are not yet illustrated.

Mice and genetic engineering mice resource are one of most important animal models in field such as current disease mechanisms research, gene functional research, medicine initiative, are also the necessary conditions of these field innovation researches.Utilize genetic engineering mice to study for each molecule link in Atheromatosis reason process, to the molecular mechanism illustrated in atherosclerosis generation evolution, the molecular target finding treatment of atherosclerosis has great importance.

Summary of the invention

The object of the invention is to determine the expression of LILRB4 and atherosclerotic mutual relation, provide one for preventing, alleviating and/or treat the novelty teabag of atherosclerotic target gene LILRB4.

Object of the present invention is achieved through the following technical solutions:

Expression and the atherosclerotic relation of the LILRB4 that the present invention determines are as follows:

1. LILRB4 gene knockout significantly increases atherosclerotic plaque area

The present invention is with ApoE gene knockout (ApoE ^-/-) mice and LILRB4/ApoE is dual-gene knocks out (LILRB4 ^-/-apoE ^-/-) mice is experimental subject, obtains atherosclerosis mouse model (AS) respectively by low fat feedstuff and high lipid food diet induced.Result shows ApoE ^-/-and LILRB4 ^-/-apoE ^-/-mice is by low fat feed diet, and aorta tree has a small amount of Mottling formation; After high lipid food diet, plaque area significantly increases, LILRB4 ^-/-apoE ^-/-the aorta tree of mice, the plaque area of aortic sinus are all significantly higher than ApoE ^-/-mice (Fig. 1-2).

2. LILRB4 gene knockout significantly reduces the stability of aortic sinus speckle

The present invention is with ApoE gene knockout (ApoE ^-/-) mice and LILRB4/ApoE is dual-gene knocks out (LILRB4 ^-/-apoE ^-/-) mice is experimental subject, obtain atherosclerosis mouse model (AS) by high lipid food diet induced, the stability of aortic sinus speckle is studied.Result shows that LILRB4 gene knockout significantly increases the content of macrophage and the area of necrotic center in aortic sinus speckle, decreases content and the collagen component content of smooth muscle cell in speckle, and entirety reduces the stability (Fig. 3) of aortic sinus speckle.

3. LILRB4 gene knockout is significantly degrading inflammatory reaction

The present invention is with ApoE gene knockout (ApoE ^-/-) mice and LILRB4/ApoE is dual-gene knocks out (LILRB4 ^-/-apoE ^-/-) mice is experimental subject, obtain atherosclerosis mouse model (AS) by high lipid food diet induced, the expression of the inflammatory factor of aortic sinus speckle is studied.Result shows that LILRB4 gene knockout significantly increases the proinflammatory factors such as ICAM-1 and IL-6 of aortic sinus speckle, reduces the expression (Fig. 4) that IL-10 etc. presses down the scorching factor.

During generation atherosclerosis known by above result, LILRB4 genetic flaw adds aorta tree plaque area, weakens the stability of aortic sinus speckle, is significantly degrading inflammatory reaction.Therefore LILRB4 has the effect suppressing atherosclerosis that development occurs, for the research atherosclerotic novel targets of control and New Policy provide theoretical foundation and Clinical Basis.

For the above-mentioned functions of LILRB4, provide the application of a kind of LILRB4, be mainly reflected in LILRB4 and prevent in preparation, alleviate and/or treat the application in atherosclerotic medicine.

One is prevented, is alleviated and/or treat atherosclerotic medicine, comprises the carrier that LILRB4 maybe can express LILRB4.

The present invention has following advantage and effect relative to prior art:

1. the present invention finds the New function of LILRB4 gene, and namely LILRB4 can significantly suppress atherosclerotic effect.

2. suppressing the function in atherosclerosis based on LILRB4, providing basis for developing atherosclerotic medicine, can be used for preparing the medicine in prevention, alleviation and/or treatment atherosclerosis.

Accompanying drawing explanation

Fig. 1 is ApoE ^-/-and LILRB4 ^-/-apoE ^-/-the aorta tree oil red O stain of mice and plaque area cartogram, result shows that LILRB4 gene knockout significantly increases the plaque area (NS: there was no significant difference) of aorta tree.

Fig. 2 is ApoE ^-/-and LILRB4 ^-/-apoE ^-/-mouse aorta hole HE dyes and plaque area statistics block diagram, and result shows that LILRB4 gene knockout significantly increases plaque area (*: the p < 0.05 vs HFD ApoE of aortic sinus ^-/-group).

Fig. 3 is ApoE ^-/-and LILRB4 ^-/-apoE ^-/-the dyeing of necrotic center, collagen component, macrophage and smooth muscle cell content and result statistics block diagram in the aortic sinus speckle of mice, result shows that LILRB4 gene knockout significantly increases the content of macrophage and the area of necrotic center in aortic sinus speckle, decreases content and collagen component content (*: the p < 0.05 vs HFD ApoE of smooth muscle cell in speckle ^-/-group).

Fig. 4 is ApoE ^-/-and LILRB4 ^-/-apoE ^-/-the expression immunofluorescence dyeing of the inflammatory factor such as ICAM-1, IL-6, IL-10 and result statistics block diagram in the aortic sinus speckle of mice, result shows that LILRB4 gene knockout significantly increases the expression of proinflammatory factor ICAM-1 and IL-6 of aortic sinus speckle, reduces expression (*: the p < 0.05 vs HFD ApoE pressing down scorching factor IL-10 ^-/-group).

Detailed description of the invention

Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.

Animal for research and raising

Laboratory animal kind, sex, age in week and source: ApoE gene knockout (ApoE ^-/-) mice and LILRB4/ApoE is dual-gene knocks out (LILRB4 ^-/-apoE ^-/-) mice, male, 8 week age, body weight 19-25g.ApoE knock out mice (ApoE ^-/-, purchased from Jackson Laboratory, article No. 002052); LILRB4 ^-/-apoE ^-/-mice is obtained by LILRB4 knock out mice (purchased from Japanese RIKEN company, article No.: RBRC02692) and the hybridization of ApoE knock out mice.

Laboratory animal feed formula: high lipid food (HFD, purchased from Fukang bio tech ltd of China, Beijing, by AIN-76 A Western Diets formula, percent of calories: protein 15.8%, fat 40%, carbohydrate 44.2%); Low fat feedstuff (NC, purchased from Fukang bio tech ltd of China, Beijing, article No. D12450B): percent of calories: protein 20%, carbohydrate 70%, fat 10%.

Animal feeding and environmental condition: all experiment mices are all raised at angiocardiopathy institute of Wuhan University SPF level Animal House (credit number: SYXK(Hubei Province): 2009-0053).Alternately illumination in every 12 hours, temperature 24 ± 2 DEG C, humidity 40%-70%, mice freely drinks water feed.

Embodiment 1 rat aorta is atherosis, and model (AS) obtains

1. laboratory animal grouping: select 8 week age, body weight 19-25g, male, ApoE ^-/-mice and LILRB4 ^-/-apoE ^-/-mice, gives high lipid food (Western Diets, HFD) respectively and low fat feedstuff (Normal chow, NC) is raised, ApoE ^-/-hFD group, ApoE ^-/-nC group, LILRB4 ^-/-apoE ^-/-hFD group, LILRB4 ^-/-apoE ^-/-nC group is totally 4 groups, often organizes each 20.

2. Atherosclerosis Model induces operating process by high lipid food:

Adopt ApoE ^-/-mice and LILRB4 ^-/-apoE ^-/-mice, sets up AS model, carries out phenotype correlation analysis, specifies the effect that LILRB4 gene pairs atheromatosis plays.Mice was from 8 week age, and the omnidistance high lipid food of HFD group is fed and put to death and collect sample for 28 weeks, and the omnidistance low fat forage feed of NC group is put to death and collects sample for 28 weeks.

Embodiment 2 AS model mice plaque area measures

1. mice last tissue sampling eventually

Mice feed high fat or low fat feedstuff until 28 weeks time, weigh, use 3% pentobarbital sodium, 90mg/kg anesthetized mice, be fixed on material drawing board with syringe needle, with the moistening mice skin of chest abdomen of gauze, cut off thoracic cavity with eye scissors, expose heart, cut off right auricle, the syringe needle of transfusion device is lunged left ventricle, slowly inject 10-15mL PBS buffer with 50mL syringe, treat that right auricle effluent is limpid, change 4% paraformaldehyde and continue to inject 10-15mL.After perfusion terminates, remove splanchnocoel internal organs, only retain heart.Under mice is placed in microscope, is separated fascia, fatty tissue around aortic arch, cut heart in ascending aorta initial part, cut off in the middle part of thoracic aorta, and about 3mm place cuts off under neck summation clavicle, aortic arch is put into above-mentioned EP pipe.

2. aorta tree plaque area measures

Aorta tree be placed in 4% paraformaldehyde overnight fixing → pure water rinsing 30min → 60% isopropyl alcohol process 10 min → oil red O dye liquor (company sigma, article No. 00625) dyeing 60min → 60% isopropyl alcohol 1min × 3 time remove remaining outer wall fat → to be laid in by tremulous pulse dye on black dissection stencil plate to clean background → anatomical lens, take pictures with digital camera after dyeing, and use Image-Pro Plus 6.0 image analysis software to carry out plaque area quantitative assay (oil red O stock solution=0.5 gram oil red O+100 milliliter 100% isopropyl alcohol, oil red O dye liquor (working solution): V(oil red O stock solution)/V(H2O)=3/2).

Aorta tree plaque area (%)=speckle gross area/aorta tree gross area * 100%.

3. pathological tissue process

3.1 paraffin specimen preparations

In 4% paraformaldehyde overnight fixing after, aortic arch filter paper is carefully wrapped, in case spill from embedding frame gap.Dewaterer is put into, 30% ethanol 15min → 50% ethanol 15min → 75% ethanol 15min → 85% ethanol 15min → 95% ethanol 15min → 100% ethanol 15min → 100% ethanol 15min → dimethylbenzene 15min → dimethylbenzene 15min → paraffin 30min → paraffin 30min after running water 30min.After aortic arch has dewatered, take out from dewaterer, aortic arch lies low in paraffin.

3.2 aortic tissue sections

Use microtome to aortic sinus paraffin specimen section (slice thickness 5 μm).

4. aortic sinus plaque area measures

Haematoxylin eosin stains (HE dyeing): get the moisture of aortic sinus paraffin white tiles 65 DEG C baking 30 minutes → dimethylbenzene 5 minutes × 3 times → 100% ethanol 1 minute → 95% ethanol 1 minute → 70% ethanol, 1 minute → pure water rinsing (slide not to hang with the globule for standard) → get rid of on most microscope slide, haematoxylin solution (Zhuhai shellfish rope, BA-4021) contaminate 5 minutes → tap water rinse (remove slide on haematoxylin loose colour) → 1% acidic alcohol 1-3 second → tap water rinse (removing acidic alcohol on slide) → Scott liquid (sodium bicarbonate 0.35g, magnesium sulfate 2g, distilled water 100mL) 1 minute → tap water embathes (removing the Scott liquid on slide) → get rid of the moisture be all on slide, Yihong solution (Zhuhai shellfish rope, BA-4024) → pure water rinsing (removing the loose colour in Yihong on slide) → 70% ethanol once → 95% ethanol once → 100% ethanol that dyes for 10 seconds 30 seconds, 3 times → dimethylbenzene 2 minutes, 3 times → take advantage of dimethylbenzene not dry time mounting (with bubble-free of cutting into slices for principle) → fume hood in dry up, microscope is taken pictures.

HE dyeing picture statistics: directly by Image-Pro Plus 6.0 image analysis software circle aortic sinus plaque area.

By aorta tree oil red O stain substantially can assess that the upper atheromatous plaque of aorta tree formed number, distribution situation and plaque area size.Fig. 1 is mice AS model aorta posterior tree oil red O stain result figure, and brachiocephalic trunk and aortic sinus are the most obvious positions of atheromatous plaque, ApoE ^-/-and LILRB4 ^-/-apoE ^-/-mice is by low fat feed diet, and aorta tree just has a small amount of Mottling formation; After being induced by high fat diet, plaque burden significantly increases, LILRB4 ^-/-apoE ^-/-the aorta tree plaque area of mice is significantly greater than ApoE ^-/-mice.Fig. 2 is mice AS model posterior sinus of Valsalva HE coloration result figure, and result shows LILRB4 after high fat diet ^-/-apoE ^-/-the plaque area of the aortic sinus of mice is also significantly greater than ApoE ^-/-mice.Above result shows that LILRB4 gene knockout significantly increases atherosclerotic plaque area.

The mensuration of embodiment 3 AS model mice plaque stability

1. the size of aortic sinus necrotic center measures

Haematoxylin eosin stains (HE dyeing), method is with embodiment 2.4, and the microscope of organizing chosen containing cholesterol crystal, acellular core fibre structure is taken pictures.

The area estimation of necrotic center: use Image-Pro Plus 6.0 image analysis software circle necrotic center area.

2. aortic sinus collagen component assay:

Picro-Sirius red (PSR) dyes, key step is: get aortic sinus paraffin white tiles 55 DEG C baking 30min → dimethylbenzene 2min, 3 times → 100% ethanol 1min → 95% ethanol 1min → 70% ethanol 1min → running water 10min → distilled water 1min → mass fraction 0.2% phosphomolybdic acid 2min → 0.1% sirius red picric acid solution drips in tissue, dye in wet box 90min → removal residual liquid → 0.01N hydrochloric acid 4s → 70% ethanol 1 time → 90% ethanol 1 time → 100% ethanol 30s, 3 times → dimethylbenzene 2min, 3 times → take advantage of dimethylbenzene not dry coverslip immediately mounting, microscope is taken pictures.

Collagen ratio measuring: use the red area of collagen of Image-Pro Plus 6.0 image analysis software circle, collagen ratio (%)=area of collagen/plaque area * 100%.

3. the expression of aortic sinus macrophage and smooth muscle cell mark measures

Immunofluorescence dyeing detects aortic sinus macrophage marker CD68, smooth muscle cell mark SMA(Smooth Muscle Actin) expression.Required primary antibodie information: CD68(MCA1957; 1:100; Rat; AbD Serotec), SMA(ab5694; 1:100; Rabbit; Abcam); Required two anti-information: Alexa Flour 568 goat anti-rat IgG(A11077; Invitrogen), Alexa Fluor 488-conjugated goat anti-rabbit IgG(A11008; Invitrogen).

Key step is:

1) roasting sheet: aortic sinus paraffin white tiles is placed in more than baking box 30min.

2) dewax: dimethylbenzene 5min × 3 time.

3) hydration: 100% ethanol 5min × 2 time; 95% ethanol 5min; 70% ethanol 5min; DdH2O embathes 5min × 2 time.

4) citrate tissue antigen recovery (Pressure method): get a certain amount of pH6.0 citrate antigen retrieval working solution in reparation box, put into pressure cooker, big fire is heated to boiling, tissue slice after dewaxing hydration is placed in and repairs box, cover pot cover, buckle pressure valve, continue to be heated to jet, after starting timing 5min, take out and repair box; Room temperature places 20min, takes out section after natural cooling.

5) ddH2O rinsing 5min × 2 time, PBS rinsing 5min × 2 time.

6) groupization stroke circle, drips 10% sheep blood serum (GTX27481, GeneTex) and closes, 37 DEG C of closed 60min in wet box.

7) abandon confining liquid, drip the primary antibodie of proper proportion dilution, 4 DEG C of overnight incubation, 37 DEG C of rewarming 30min, discard primary antibodie, PBS washes 10min × 3 time.

8) drip two to resist, in wet box, hatch 60min for 37 DEG C, discard two and resist, PBS embathes 5min × 3 time.

9) SlowFade Gold antifade reagent with DAPI(S36939, Invitrogen) mounting.

10) viewed under fluoroscopy, takes pictures.

Fluorescent quantitation is added up: use Image-Pro Plus 6.0 image analysis software to measure Positive Cell Counts, CD68/SMA(%)=positive cell number/plaque area * 100%.

Macrophage is most important cell component in speckle, it mainly contain blood circulation mononuclear cell enter interior subcutaneous after differentiate, Monocytes/Macrophages can secrete multiple adhesion, chemotactic factor as cell adhesion molecule (ICAM-1), MCAF (MCP-1) etc., promote entering of speckle inner cell, in addition macrophage also can secrete multiple matrix metalloproteinase (MMPs), reduce area of collagen in speckle, thus destroy the stability of speckle; Smooth muscle cell can secrete various kinds of cell substrate, is the cell source of atheromatous plaque endocrine collagen, and Main Function repairs destroyed cellular stromal component thus plays a protective role; Collagen component is most important extracellular matrix in speckle, is also the main component of fibrous cap, and having anti-blood flow and impact the effect preventing plaque rupture, is also the important evaluation index safeguarding plaque stability.

Fig. 3 is ApoE ^-/-mice and LILRB4 ^-/-apoE ^-/-the analysis result figure of the aortic sinus speckle inclusions of mice.Aortic sinus HE dyes visible LILRB4 ^-/-apoE ^-/-the necrotic center area of mice is obviously greater than ApoE ^-/-mice; PSR coloration result shows the model after high fat diet, LILRB4 ^-/-apoE ^-/-the collagen ratio of group mice is starkly lower than ApoE ^-/-mice; Immunofluorescence is sent out and is observed macrophage marker CD68 at LILRB4 ^-/-apoE ^-/-expression in mice speckle is then significantly higher than ApoE ^-/-mice; Smooth muscle cell mark SMA is at LILRB4 ^-/-apoE ^-/-expression in mice speckle is starkly lower than ApoE ^-/-mice.Above result shows that LILRB4 gene knockout significantly increases the content of macrophage and the area of necrotic center in aortic sinus speckle, reduces content and the collagen component content of smooth muscle cell in speckle; Therefore LILRB4 gene knockout can reduce the stability (Fig. 3) of aortic sinus speckle.

The mensuration of inflammatory Cytokines Expression in embodiment 4 AS model mice speckle

Immunofluorescence dyeing detects the expression of the inflammatory factors such as aortic sinus ICAM-1, IL-6, IL-10.Required primary antibodie information: ICAM-1(AF796; 1:100; Goat; R & D systems), IL-6(AF-406-NA; 1:100; Goat; R & D systems), IL-10(AF-217-NA; 1:100; Goat; R & D systems); Required two anti-information: Alexa Flour 568 donkey anti-goat IgG(A11057; Invitrogen).

Key step is: see embodiment 3.3.

Fluorescent quantitation is added up: use Image-Pro Plus 6.0 image analysis software to carry out absorbance (IOD) to positive cell and measure.

Inflammatory reaction is one of principal element causing atheromatous plaque to break.The a large amount of cytokine of proinflammatory cytokine secretion is as interleukin-6 (IL-6), and interleukin 10 (IL-10) etc. can cause the progress of the activation of endotheliocyte, the hypertrophy apoptosis of smooth muscle cell and medicated porridge sample pathological changes.Adhesion, the chemotactic factor of impaired endotheliocyte and Monocytes/Macrophages secretion are the key factors that transmitting inflammation cell is assembled to atheromatous plaque.The expression change of the inflammatory factors such as ICAM-1, IL-6, IL-10 is observed by immunofluorescence dyeing, result shows that LILRB4 gene knockout significantly improves the expression of proinflammatory factor ICAM-1, IL-6 in aortic sinus speckle, reduces the expression (Fig. 4) that IL-10 presses down inflammatory factor.

Above-described embodiment result shows, ApoE ^-/-mice and LILRB4 ^-/-apoE ^-/-mice issues lively pulse atherosclerosis in the induction of high fat diet, and ApoE ^-/-mice is compared, and the dual-gene Aortic Plaque area knocking out rear mice of LILRB4/ApoE significantly increases, and plaque stability reduces, and inflammatory reaction obviously increases the weight of.These results show, LILRB4 significantly can suppress the formation of Aortic Plaque and atheroscleroticly to develop.Present invention demonstrates that LILRB4 has important protective effect in Atherosclerosis Model, its medicine that can be used for preparing prevention, alleviate and/or treat atheromatosis.

Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.

Claims (2)

1. a LILRB4 prevents in preparation, alleviates and/or treat the application in atherosclerotic medicine.

2. prevent, alleviate and/or treat an atherosclerotic medicine, it is characterized in that: comprise the carrier that LILRB4 maybe can express LILRB4.