CN104222518A - Compositions and methods for treating and preventing weight-related disorders in companion animals - Google Patents
Compositions and methods for treating and preventing weight-related disorders in companion animals Download PDFInfo
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- CN104222518A CN104222518A CN201410445898.3A CN201410445898A CN104222518A CN 104222518 A CN104222518 A CN 104222518A CN 201410445898 A CN201410445898 A CN 201410445898A CN 104222518 A CN104222518 A CN 104222518A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
Abstract
The invention encompasses compositions and methods for treating or preventing disorders in companion animals, wherein the compositions and methods include feeding the companion animal a composition including lipoic acid or a salt thereof.
Description
The application is the applying date is on December 29th, 2009, and application number is 200980153832.4, denomination of invention for " be used for the treatment of and prevent companion animals with the composition of weight-associated disease and method " the divisional application of application for a patent for invention.
Invention field
The present invention includes that treat and/or prevent companion animals with composition that is weight-associated disease and method, wherein said composition and method comprise feeding to companion animals and comprise the composition of lipoic acid or its salt.
background of invention
An important indication of animal health is the Body composition of animal.Unsound diet and/or unsound life style can cause animal to have the body fat of unhealthy ratio, especially relevant to the lean meat in body.Now think that the human body of (weight) shows that this animal is unhealthy to fat mass more than 30%, if especially body fat amount is more than 35% time (weight).
The present invention comprises the pet food composition of companion animals, and it is than companion animal food in the market, for maintenance or promotion health Body composition, has treatment and the prevention effect of raising.
summary of the invention
The present inventor develops the method being used for the treatment of or preventing the food compositions of Animal diseases and use described composition.
The present invention includes the method keeping or promote that the healthy body of companion animals is formed, such as lose weight or body fat, increase the percentage of cutability, described method comprises and effectively to keep to detoxification or the composition of amount of promotion health Body composition, and described composition comprises lipoic acid or its salt.
detailed Description Of The Invention
summation
Present invention resides in the food composition of prevention or disease therapy in companion animals in need, it includes lipoic acid or its salt of effective amount.
Embodiment comprises a kind of maintenance in companion animals or the method for promotion health Body composition, and it comprises and effectively to keep to this detoxification or the composition comprising lipoic acid or its salt of amount of promotion health Body composition.
In certain embodiments, described effective dose is at least about 25ppm.
In certain embodiments, described effective dose is at least about 50ppm.
In certain embodiments, described effective dose is at least about 100ppm.
In certain embodiments, described effective dose is about 100ppm to about 600ppm.
In certain embodiments, described effective dose is about 100ppm to about 200ppm.
In certain embodiments, described companion animals is dog.
In certain embodiments, described companion animals is cat.
In certain embodiments, described composition also comprises protein, fat, carbohydrate, fiber and combination thereof.
In certain embodiments, described composition is dog based food.
In certain embodiments, described composition is cat based food.
In certain embodiments, described composition is food, nutriment, replenishers, animal training food (treat) or toy.
In certain embodiments, described composition is moist food form.
In certain embodiments, described composition is dry food form.
Another embodiment comprises and a kind ofly in companion animals, causes the method lost weight, and it comprises the composition comprising lipoic acid or its salt effectively causing the amount lost weight to this detoxification.
In certain embodiments, described effective dose is at least about 25ppm.
In certain embodiments, described effective dose is at least about 50ppm.
In certain embodiments, described effective dose is at least about 100ppm.
In certain embodiments, described effective dose is about 100ppm to about 600ppm.
In certain embodiments, described effective dose is about 100ppm to about 200ppm.
In certain embodiments, described composition also comprises protein, fat, carbohydrate, fiber and combination thereof.
In certain embodiments, described composition is dog based food.
In certain embodiments, described composition is cat based food.
In certain embodiments, described composition is food, nutriment, replenishers, animal training food or toy.
In certain embodiments, described composition is moist food form.
In certain embodiments, described composition is dry food form.
In certain embodiments, described companion animals is dog.
In certain embodiments, described companion animals is cat.
Another embodiment of the invention is included in companion animals the method causing body fat and consume, and it comprises the composition of the present invention comprising lipoic acid or its salt effectively causing the amount of body fat consumption to this detoxification.
In certain embodiments, the effective dose of lipoic acid is at least about 25ppm.
In certain embodiments, described effective dose is at least about 50ppm.
In certain embodiments, described effective dose is at least about 100ppm.
In certain embodiments, described effective dose is about 100ppm to about 600ppm.
In certain embodiments, described effective dose is about 100ppm to about 200ppm.
In certain embodiments, described composition also comprises protein, fat, carbohydrate, fiber and combination thereof.
In certain embodiments, described composition is dog based food.
In certain embodiments, described composition is cat based food.
In certain embodiments, described composition is food, nutriment, replenishers, animal training food or toy.
In certain embodiments, described composition is moist food form.
In certain embodiments, described composition is dry food form.
In certain embodiments, described companion animals is dog.
In certain embodiments, described companion animals is cat.
Another embodiment of the invention is included in companion animals the method increasing cutability percentage, and it comprises the composition of the present invention comprising lipoic acid or its salt effectively increasing the amount of cutability percentage to this detoxification.
In certain embodiments, the effective dose of lipoic acid is at least about 25ppm.
In certain embodiments, described effective dose is at least about 50ppm.
In certain embodiments, described effective dose is at least about 100ppm.
In certain embodiments, described effective dose is about 100ppm to about 600ppm.
In certain embodiments, described effective dose is about 100ppm to about 200ppm.
In certain embodiments, described composition also comprises protein, fat, carbohydrate, fiber and combination thereof.
In certain embodiments, described composition is dog based food.
In certain embodiments, described composition is cat based food.
In certain embodiments, described composition is food, nutriment, replenishers, animal training food or toy.
In certain embodiments, described composition is moist food form.
In certain embodiments, described composition is dry food form.
In certain embodiments, described companion animals is dog.
In certain embodiments, described companion animals is cat.
The term " companion animals " used in the present invention comprises any non-human animal being suitable as the raising pets of people, comprises dog, cat and rodent.All aspects of the invention are all preferred for treatment cat and/or dog.
Term " dog " comprises those dogs as companion animals, as domesticated dog, working dog etc.Term dog and term dog synonym.
Term " cat " comprises those cats as companion animals, and these companion animals are called domestic cat or raise cat.
Term " rodent " includes but not limited to hamster, mouse, rat, cavy, gerbil jird, hare, hedgehog, ferret, silk hair rabbit etc.
Unless otherwise specified, all percentages represented herein are all in the weight ratio of the composition of dry product.
the present composition
One embodiment of the invention comprises the composition for companion animals, and it includes lipoic acid or its salt of effective amount.
Term used herein " lipoic acid or its salt " includes but not limited to racemic mixture, lipoate, ester, the acid amides or derivatives thereof of such as alpha-lipoic acid, lipoic acid, the such as U.S. patent No. 5,621, and described in 117.In various embodiments, lipoic acid can be given with the composition forms comprising humidity or dry food composition, it can be the form of moist food, dry food, replenishers or training food.Lipoic acid can be incorporated into wherein or be incorporated on the surface of any food composition, as by spraying or condensing on it, or by snacks, replenishers, training food or join in diet in the mode in the liquid part (as water or other fluid) of diet.Can by lipoic acid with powder, solid or give with liquid (comprising gel) form.An important aspect can be supplied with the lipoic acid of effective amount to provide positive effect to animal.Usually, lipoic acid source can keep being present in described composition the avirulent amount of animal with maximum.
The amount of alpha-lipoic acid can change, from least about 25ppm, about 50ppm, about 100ppm, about 200ppm, about 300ppm, about 500ppm, about 700ppm, about 900ppm, about 1100ppm, about 1200ppm, about 1400ppm, about 1600ppm, about 1800ppm, about 2000ppm, about 2200ppm, about 2400ppm, about 2600ppm, about 2800ppm, about 3000ppm or about 3500ppm.In various embodiments, the scope of the lipoic acid of dog can be given for about 150ppm to about 4500ppm.In various embodiments, the scope of the lipoic acid of cat can be given for about 65ppm to about 2600ppm.In some exemplary, the amount given can about 100ppm to keep to the avirulent amount of pet between change.In other embodiments, scope is about 100ppm to about 200ppm.
In various embodiments, the food composition comprising lipoic acid provides a kind of basic full trophism diet to calculated animal subject." full trophism diet " is following diet: it is included in sufficient nutrition food maintaining healthy animal normal healthy state.
Lipoic acid or its salt exist with the concentration that the health of the animal to plan is harmless.Therefore, such as, lipoic acid or its salt exist not cause the concentration of bad or toxic action.
Described composition can be liquid or solid food.When composition is liquid, lipoic acid or its salt can be mixed with other component.When composition is solid, can lipoic acid be coated on composition, mixes in composition or both.
In various embodiments, lipoic acid or its salt can be joined in animal food.In various embodiments, lipoic acid or its salt can be joined in animal food in certain place by makers-up or producer, or be joined in animal food by Animal breeders before feeding animals.In various embodiments, lipoic acid or its salt can be added in processing animals cooking cycle, as between other component mixing period of composition and/or add afterwards, then pack and consumer can be obtained.This processing can comprise extrudes, can processed, to cure etc. or any known other in this area prepares method or the process of pet food.In various embodiments, lipoic acid or its salt can derive from the natural origin of such as animal or plant component, or lipoic acid or its salt can be obtained by the source coming from synthesis, or lipoic acid or its salt can come from mixture that is natural and synthesis source.
Except lipoic acid or its salt, described composition also comprises the component that at least one is applicable to companion animals consumption, includes but not limited to fat, carbohydrate, protein, fiber, feed with balanced nutrients, as vitamin, mineral matter and trace element and composition thereof.Those of ordinary skill in the art all can according to the dietetic requirement of animal, and the kind of such as animal, age, volume, body weight, health status and function, select quantity and the COF type of general food.
The food ingredient part of food composition can comprise any specific COF being up to about 100% or the mixture of COF that can comprise different proportion.In certain embodiments, food composition comprises the combination of the COF of following amount, and protein, about 0wt.%-that carbohydrate, about 0wt.%-that fat, about 0wt.%-that about 0wt.%-is about 50wt.% are about 75wt.% are about 95wt.% are about one or more feed with balanced nutrients that the dietary fiber of 40wt.% and about 0wt.%-are about 15wt.%.
In certain embodiments, fat and carbohydrate food ingredient obtain from multiple source, as animal tallow, fish oil, vegetable oil, meat, meat products, cereal, other animal or plant source and composition thereof.Cereal comprises wheat, corn, barley and polished rice.
In certain embodiments, protein food ingredient obtains from various source, as plant, animal or both.Animal protein comprises meat, meat byproduct, cheese and eggs.Meat comprises poultry, the animal flesh of the flesh of fish and such as ox, pig, sheep, goat etc., meat byproduct comprise lung, kidney, brain, liver, stomach and intestines.Protein food ingredient can not conform to amino acid and/or peptide yet.Protein food ingredient preferably includes meat, meat byproduct, dairy products or eggs.
In certain embodiments, fiber food composition obtains from various source, as lignocellulosic fiber source, and such as cellulose, sweet tea grounds, peanut shell and fibre and soya.
In certain embodiments, feed with balanced nutrients obtains from the multiple source known to person skilled in the art, such as vitamin and mineral supplements and COF.Vitamin and the mineral matter avoiding lacking and maintain healthy requirement can be comprised.Be easy to find in this tittle this area.The National Research Council (NRC) provides this kind of nutrient of the domestic animal of recommended amounts.See, nutritional need (the 10th Rev.Ed. of such as pig, Nat'l Academy Press, Wash.D.C., 1998), nutritional need (the 9th Rev.Ed. of poultry, Nat'l Academy Press, Wash.D.C., 1994), nutritional need (5th Rev.Ed., the Nat'l Academy Press of horse, Wash.D.C., 1989).U.S. feed intendant association (AAFCO) provides the recommended amounts of this kind of nutrient of dog and cat.See American Feed Control Officials, Inc., Official publication, pp.129-137 (2004).Usually the vitamin that can be used as food additive comprises vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin D, biotin, vitamin K, folic acid, inositol, nicotinic acid and pantothenic acid.The mineral matter and the trace element that can be used as food additive comprise calcium, phosphorus, sodium, potassium, magnesium, copper, zinc, chloride, iron, selenium, iodine and iron.
In certain embodiments, described food composition can comprise other composition, the analogous components that such as vitamin, mineral matter, filler, palatability improving agent, adhesive, spices, stabilizing agent, emulsifying agent, sweetener, colouring agent, buffer, salt, coating and technical staff understand.Stabilizing agent comprises the material being tending towards increasing the composition shelf life, as anticorrisive agent, synergist and chelating agent, packaging gas, stabilizing agent, emulsifying agent, thickener, gelling agent and NMF.The example of emulsifying agent and/or thickener comprises gelatin, cellulose ether, starch, starch ester, starch ether and converted starch.The specified quantitative of each composition component, COF and other composition will depend on many factors, the specific components comprised as composition and composition; Animal species; Age of animal, body weight, health status, sex and diet; The consumption rate of animal; The disease treated or illness type etc.Therefore, the amount of described component and composition can change in wide range, and can depart from preferred ratio as herein described.
In an exemplary embodiment, except lipoic acid or its salt, composition also can comprise such as at least following a kind of composition:
The carbohydrate of (a) about 0%-about 75%,
The fat of (b) about 2%-about 50%,
The dietary fiber of (c) about 0%-about 40%, and
One or more feed with balanced nutrients of (d) about 0%-about 15%.
The diet of adult companion pets of feeding (as canid and cats) is to the standard normal diet of this age feeding animal.It is below the typical diet of 1-6 age, dog year.
table 1: exemplary companion animals pet food compositions
| Composition | Target |
| Protein (% dry product) | 23 |
| Fat (% dry product) | 15 |
| Phosphorus (% dry product) | 0.6 |
| Sodium (% dry product) | 0.3 |
Described composition can comprise other composition for keeping or improve animal health, as the medicine and composition etc. of replenishers, medicine, herbal medicine, whole body therapeutic.
Composition of the present invention can comprise one or more for preventing or treat other composition of one or more diseases or illness.
The component realized in the food of this point is antioxidant or its mixture.Antioxidant is the material of quench free radicals.The example of this kind of material comprises food; as ginkgo, citrus pulp, treaster, tomato pomace, carrot and spinach; allly all be preferably dry product; and other material various, as beta carotene, selenium, Co-Q10 (ubiquinone), lutein, tocotrienols, isoflavones, S-adenosylmethionine, glutathione, taurine, N-acetylcystein, vitamin E, vitamin C, alpha-lipoic acid, VBT etc.Vitamin E can be given, as ester such as vitamin e acetate, succinate, palmitate etc. with the form of tocopherol or tocopherol mixture and its various derivative.Preferred α type, but β, γ and δ type can be comprised.Preferred d type, but racemic mixture also can accept.Described various and derivative plays a role with vitamin E-like activity after pet picked-up.Vitamin C in this diet can ascorbic acid and various derivative thereof, and the form as calcium phosphate, cholesterol salt, 2-monophosphate etc. gives, its after pet picked-up with the effect of vitamin C sample activation plays.They can be any forms of such as liquid, semisolid, solid and heat-stable form.Can by alpha-lipoic acid with alpha-lipoic acid or as U.S. Patent number 5,621, lipoate (salt) derivative, its racemic mixture, salt, ester or amide form thereof in 117 join in food.VBT can be joined in diet, and the various derivatives of carnitine can be used, as salt, example hydrochloric acid salt, fumarate and succinate, and acetylated carnitine etc.
Calculate the amount of the active material added in diet, all amounts are all in the wt% of food (dry product), and active material itself measures as dissociant.The maximum used should not produce toxicity.Can use at least about 100ppm or the vitamin E at least about 150ppm.Spendable preferred scope is about 500 to about 1,000ppm.Although unnecessary, be generally no more than the maximum of about 2,000ppm or about 1,500ppm.For vitamin C, use at least about 50ppm, ideal is at least about 75ppm, and better is at least about 100ppm.Atoxic maximum can be used.The amount of alpha-lipoic acid can change from least about 25ppm, and ideal is at least about 50ppm, and better is about 100ppm.Maximum can change between the avirulent amount of pet from about 100ppm to maintenance.Preferred scope is that about 100ppm-is about 200ppm.For VBT, about 50ppm, ideal is about 200ppm, the minimum that better to be about 300ppm be canid is used.To cats, the VBT of slightly high minimum can be used, 100ppm, 200ppm and 500ppm according to appointment.Non-toxic maximum can be used, such as, be less than about 5,000ppm.For canid, slightly low amount can be used, such as, be less than about 5,000ppm.For canid, preferred scope is about 200ppm to about 400ppm.For cats, preferred scope is about 400ppm to about 600ppm.The beta carotene of about 1-15ppm can be used.The selenium of about 0.1 to about 5ppm can be used.The lutein at least about 5ppm can be used.Can use at least about 25ppm tocotrienols.Can use at least about 25ppm Co-Q10.The S-adenosylmethionine at least about 50ppm can be used.The taurine at least about 1000ppm can be used.The isoflavones at least about 25ppm can be used.Can use at least about 50ppm N-acetylcystein.The glutathione at least about 50ppm can be used.The ginkgo biloba extract of at least 50ppm can be used.
Below the material composition with higher ORAC (oxygen radical absorbability) content: spinach slag, tomato pomace, oranges and tangerines meat, treaster, carrot grain, broccoli, green tea, ginkgo and cereal seitan meal.
When joining in food with 1% inclusion (for reducing ORAC composition, total amount is 5% sub, as corn), they add the ORAC content of overall food, and adding the ORAC content of the blood plasma of the edible animal containing these component foods.If to add in food with 1% (adding 5% altogether) of four kinds of other 1% composition combinations, every gram of dry product preferably can be used to have any composition of the anti-oxidant equivalent (Trolox equivalents) of ORAC content > 25 μm of ol.In certain embodiments, at least one that described composition also includes effective amount is selected from following material: aminoglucose, chondroitin, chondroitin sulfate, dimethyl sulfone (" MSM "), creatine, antioxidant, Perna canaliculata, omega-fatty acid, ω-6 aliphatic acid and composition thereof.
In various embodiments, the replenishers including effective amount lipoic acid or its salt also include at least one material of effective amount, comprise aspirin, antiinflammatory as brufen, cox 2 inhibitor and other medical and Pharmaceutical composition and combination thereof.Replenishers include but not limited to and the feed that another kind of feed uses, in order to improve the performance of nutrient balance or entirety.Replenishers comprise composition, and this based composition as a supplement agent is not diluted in other feed, and it can give separately, unrestricted choice together with the other parts in the quantitative food of animal, or dilute by animal conventional food and be mixed and made into complete food.P.220, such as AAFCO, at American Feed Control Officials, Inc.Official Publication, provides the discussion about replenishers in (2003).Replenishers can be various forms, comprise the composition of such as powder, liquid, syrup, pill and encapsulating.
In certain embodiments, described composition can be training food.Training food is included in non-meal time and gives animal to lure the composition of animals eat, such as dog dog bone.Training food can have trophism, and wherein said composition comprises one or more nutrients and can have food sample composition.The training food of non-nutritive comprises other non-toxic training food any.Can composition or component be coated on training food, mix among training food or both.Training food of the present invention is by being similar to used the extruding or baking method preparation of dry food.Also other method can be used composition to be coated in the outside of existing training food forms or to be expelled to by composition among existing training food forms.
In certain embodiments, described composition can be toy.Toy comprises can chew toys, as artificial bone-head.Lipoic acid or its salt on toy surface or can form coating on the surface of toy part, or it to be partly or entirely incorporated in toy or both.In one embodiment, lipoic acid or its salt are that anticipated user is easy to oral.There is the toy of a lot of current listing be applicable at present, such as, U.S. Patent number 5,339,771, U.S. Patent number 5,419,283 and bibliography disclosed in it thereof.The invention provides part can expendable toy, such as, comprise the toy of plastic part, and completely can expendable toy, as greenhide and various artificial bone-head.The toy that the present invention preferably provides dog or cat to use.
the preparation of the present composition
Adopt routine known to the skilled to prepare the method for food, the present composition can be prepared into the form of can or wet mass.General will on the ground animal proteinaceous tissues and other composition, as fish oil, grain, equilibrium composition, specific purposes additive (as vitamin and mineral mixture, inorganic salts, cellulose and sweet bitter edible plant slag, extender etc.) and mix in order to the water of processing capacity enough.These compositions are being suitable for mixing in the container heated, these components of blending simultaneously.Any applicable mode is used to heat this mixture, as directly injected steam or the container with installation heat exchanger.Then add last composition, mixture is heated to about 50 °F of-Yue 212 °F of temperature.This extraneous temperature is receivable, but may be commercially unpractiaca under not using other processing aid.When being heated to proper temperature, material will be generally thick liquid form.This thick liquid is filled in can.Cover lid, and by this seal of vessel.Then the can of sealing is placed in the conventional equipment be designed for content sterilizing.Sterilizing is usually by completing heating temperatures to more than about 230 °F appropriate times, and the heat time depends on temperature used, composition and similar factor.The present composition can before the preparation of food composition, period or add afterwards.
Adopt conventional method known to the skilled, food composition can be prepared into dry form.General the dry ingredients such as such as animal protein, vegetable protein, cereal are ground and mix.Then add humidity or liquid component, comprise fat, oils, animal protein, water etc. and mix with drying composite.Then this mixture is processed into kibble (kibbles) or similar dry small pieces.Kibble adopts extrusion method to be formed usually, wherein mixture that is dry and humid fraction is carried out machining at elevated pressures and temperatures, and is forced through little opening, be then cut into kibble by rotary cutter.Then wet kibble is the dry and one or more Topcoating of optional coating, as spices, fat, oils, powder etc.Also can adopt baking method instead of extrusion method, make kibble by dough/pasta (dough), wherein before dry heat processing, dough/pasta is placed in mould.Adopt those that be similar to above-mentioned dried foods or toy to extrude or baking method, as U.S. Patent number 5,339,771 and 5,419,283 those disclosed, described food composition can be training food forms.Before, during, or after preparation, composition of the present invention can be joined in food composition.
by present composition treatment or prophylactic method
The present invention also comprises and includes the lipoic acid of effective amount or the composition of its salt by giving companion animals in need, treats or prevent the method for some disease.
In companion animals pet food, add a certain amount of antioxidant or its mixture can keep or promote pet, as the healthy body in adult pet is formed, such as, lose weight or body fat, and increase the percentage of cutability.Term " is grown up " cats in the canid that generally refers at least 1-6 year and at least 1-6 year.Aged dog or cat be 7 years old and more than.
The present inventor successfully realizes keeping or promotion health Body composition, such as, lose weight or body fat, or increases the percentage of cutability.
The invention is not restricted to specific methodology as herein described, scheme and reagent, reason is that they all can change.In addition, term object used herein is only to describe specific embodiment, is not intended to limit scope of the present invention.Singulative " one " used and " being somebody's turn to do " herein and in additional claims, except clearly indicating in context, otherwise all comprise plural referents.Similarly, word " comprises " and " comprise ground " should be interpreted as being included and nonexcludability.Unless defined otherwise, all technology used herein and scientific terminology and any acronym all have the identical implication usually understood with those skilled in the art in field of the present invention.Although any composition that is similar with described herein or that be equal to, method, manufacture article or other means or material can be used in the embodiment of this invention, preferred composition, method, manufacture article or other means or material be described herein those.
Embodiment
The present invention illustrates further by the embodiment of its following preferred embodiment, although be understood that these included embodiments are only for illustrating, and unless otherwise specified, they not delimit the scope of the invention.
Embodiment 1
The adult dog of this research use 30 random source is carried out.Dog is at least 10 monthly ages, conceived, non-lactation have suitable body weight before the test.Animal is divided at random 5 dietary therapy groups, often organize 3 male and 3 female.
2 weeks ingest early stage (table 2), meet to all dog feedings or exceed that Official US's feed control association (AAFCO 2000) advises the control Food (adding 0ppm DL-alpha-lipoic acid) of nutritious recommended amounts.After this ingests early stage, dog is divided at random 5 treatment groups, comprises one of following DL-alpha-lipoic acid target (in dry product) respectively: 0ppm, 150ppm, 1,500ppm, 3,000ppm, 4,500ppm.In all diet, contrast and alpha-lipoic acid, all add vitamin E, and its amount is 600-1000 international unit level, and adds the vitamin C that level is 100-200ppm.
In addition to water, test food is unique source of nutrition.Fresh water arbitrarily supplies.Select dog and after taking initial body weight, according to the food ME of expection, calculate the food dosage of each dog.The calculating of initial food dosage is with the maintenance energy requirement (MER) of dog for foundation, and MER, by illustrating that the factor of normal activity corrects, is calculated as follows:
MER (kcal/ day)=1.6 × RER (static energy requirement)
Wherein: RER (kcal/ day)=70 × body weight (kg) 0.75.
Weigh to dog weekly, regulate food dosage on demand, to make the enough food of feeding to keep the most fit heavy of them.Measure the most fit heavy be 3 points of 5 fractions.If after adjustment food dosage, the body weight of dog do not maintain original body mass-10% within, then dog is got rid of from research.Record the measured value of all body weight and food intake.
Sample is ground, by 0.100+/-0.001g sample extraction 2 times to 5.0ml phosphate buffer (10mM Na
2hPO
4, 2mM ethylenediamine tetra-acetic acid (EDTA), 0.9%NaCl, pH7.4) in.250 μ L extracts are placed in the glass centrifuge tube of 5ml with the linear pipe cap of Teflon.Add the 5mM dithioerythritol (DTE) of 15 μ L EDTA solution (100mM EDTA is adjusted to pH 7.8 with about 1M NaOH) and 50 μ L brand-news.This solution vortex is also at room temperature hatched 5 minutes.Then 10 μ L 1M H are added
3pO
4with 2.0ml ether.By each pipe block, vortex at room temperature with 1500 × g centrifugal 3 minutes.Ether layer is transferred in independent 5ml glass centrifuge tube, water layer 1.5ml ether is extracted 2 times more simultaneously.Merge all extracts of same sample.Then at room temperature, in a water bath at vaporized nitrogen device inner drying extract.At this point, by cover for sample and freeze overnight.
Then the extract of drying is thawed, make solution with the 1mMDTE of 70 μ L SDS/EDTA solution (0.11% lauryl sodium sulfate (SDS), 15mM EDTA, 0.9%NaCl) and 5 μ L brand-news.Subsequently by the NaBH of 50 μ L brand-news
4join in each pipe.Each pipe vortex is also at room temperature hatched 10 minutes.After 10 minutes, sample is freezing at-70 DEG C.Add 20 μ L2M HCl, then solution is thawed.After solution thaws, add 800 μ L 100mM NH
4hCO
3.By solution vortex, and add the acetonitrile solution (mBBr) of 5 μ L 100mM monobromo diamines (monobromodiamane).Then at room temperature, this solution is in the dark hatched 90 minutes.
By extracting with 1.5ml carrene, from the sample after hatching, remove excessive mBBr and DTE derivative.Water layer is placed on HPLC.Separate sulfur is sad, and use mobile phase, this mobile phase is made up of 30% acetonitrile, 1% acetic acid, with about 2M NH
4oH is adjusted to pH3.95, with flow velocity 1.0mL/min pumping, and each sample introduction isocratic elution 15 minutes.The density that food is extruded in this preparation presumption equals 1g/ml.
Starting to carry out to study front 2 weeks and at the 0th, 28,56,84,112,140 and 168 day that studies, sterile collection whole blood carried out complete blood count and blood biochemical analysis.In addition, carrying out the 0th, 28 and 84 day of diet intervention, collecting 15ml whole blood and carry out separation of lymphocytes.
Heparinised whole blood is divided and is placed in 50ml Accuspin conical centrifuge tube (Sigma Chemical), then add isopyknic phosphate buffered saline (PBS) (PBS).Turn down depressed, by sample with 700g centrifugal 30 minutes.Collecting monocytic cell layer, is transferred in 15ml conical centrifuge tube, settling flux in the PB of 1-3ml, centrifugal as before (first clean).Second time cleaning is carried out by cleaning step first.Finally, collecting cell, is suspended in perchloric acid (10%w/v), freezing use to be analyzed at-70 DEG C.
Sample is transferred to from-70 DEG C of refrigerators the inside with in the cooler of dry ice.In refrigerated centrifuge, by each pipe with 12,000rpm centrifugation 5 minutes.One equal portions are carried out the sample that glutathione (GSH) analyzes to be transferred in conical tube.
The method of the Reed improved according to Jones (Jones etc.) and its colleague (Fariss etc.), carries out the derivatization of described acid dissolve extract.
Say briefly, 150 μ L external standard extracts are joined in 1.5ml microcentrifugal tube, then add 20 μ γ-Clu-Glu internal standard compounds and 50 μ L IAA, then mix.Use KOH-KHCO
3working solution, is adjusted to pH and is about 10 (purples) by this solution.At room temperature, solution is hatched 1 hour in dark place.Add the sanger's reaction with cumulative volume same volume, then at room temperature, by this solution in dark place overnight incubation (20 hours).
After hatching, by solution with 12,000rpm centrifugation 5 minutes, supernatant is transferred in another 1.5ml microcentrifugal tube.200 μ L supernatants are joined in the amber non-syringe-driven filter (Autovial) with 300 μ L entrances, fix top to carry out HPLC analysis with crimper.
Adopt the solvent described in (Fariss, Jones) and separation condition.Quantitatively determine the level of GSH and GSSG relative to real reference material.Use γ-glutaminyl-glutamate as internal standard compound assessment derivatization efficiency.
Clinical chemistry, hematology and body weight are analyzed by paired t-test relative to the comparison of baseline value, and SAS window significant is set to P < 0.05.Be separated the mean value of each minute point by One-way ANOVA ANOVA, significant is set to P < 0.05.The difference of between each group between 84 days and baseline GSH: GSSG is analyzed by unidirectional ANOVA, and SAS window significant is set to P < 0.05.
Result
Continuous 7 mensuration (0,28,56,84,112,140,168 days) concentration (ppm) of lipoic acid is within desired mensuration susceptibility and the manufacturing parameter scope that generally runs in our equipment (table 2) in the food that records.
Food intake data are not remarkable.It is more when all starting than research that the food of picked-up during the most animals in all groups 6 months is average.Except occurring that some lose weight at first in 4,500ppm inclusion group, but this change presented outside reverse through 6 months, and weight data is not remarkable.The scoring of physical qualification seems not by the impact of the less loss of this body weight.
Conventional physical examination does not expose the evidence of any abnormal conditions relevant to nutrition or DL-alpha-lipoic acid toxicity.All animals in Research Group all keep normal during whole research.In several animals, vomiting is once in a while found during research; But, do not observe cause people to draw vomiting may be the trend of this conclusion caused by lipoic acid.An animal in the highest inclusion group 21 days time because of lose weight and leukocytosis and from research remove.At the end of research, this animal white cytosis is not eliminated, and therefore infers and is attributed to some other lysis.
When by 28,56,84,112,140, serum biochemistry value compared with the initial value of identical group of dog with 168 days, notice several difference statistically, but, do not think that these have meaning biologically, because these values are in or closely laboratory reference value scope, and notice and to be consistent within the several months.More also some significant differences are demonstrated between each time limit internal reference group and other treatment group, but, do not think that these have meaning biologically, because these values are in or closely clinical labororatory's reference range, and do not occur trend.
When comparing the 28th, 56,84,112,140 with the initial value of identical group of dog with the blood values of 168 days, notice some differences statistically, but, do not think that these have meaning biologically, because these values are in or closely laboratory reference value scope, and do not occur trend.More also some significant differences are demonstrated between each time limit internal reference group and other treatment group, but, do not think that these have meaning biologically, because these values are in or closely clinical labororatory's reference range, and do not occur trend.
GSH: GSSG ratio
The obvious entire effect (P=0.024) of the change display food of GSH: GSSG ratio in feeding 84, and all this ratios of fill-in group increase (table 3).ANOVA demonstrates the significant difference of minimum and the highest inclusion food compared with basal diet, but the maximum of increase is in minimum inclusion level.That is, the change observed of the change of GSH: GSSG ratio of the highest and minimum inclusion and basal diet same time time limit has notable difference.Within 84th, fail to detect the ratio of 4 points, because all GSSG do not detected in these samples any (1 control group, 3 treatment groups).Therefore, if this method of testing sensitivity is enough to mensuration low-level GSSG on the 84th, then GSH: the GSSG ratio value that fill-in group is even higher can be shown.
table 2
table 3
* 1 dog in control group on the 84 and 4,500ppm group does not detect GSSG, 2 dogs simultaneously in 3,000ppm groups on the 84th do not detect GSSG.
The further observation to alpha-lipoic acid can be applied.In food, chronic supply alpha-lipoic acid is safe and efficient.It improves the ratio of reduced glutathione (GSH) and oxidized form of glutathione (GSSG).In food, the chronic period giving alpha-lipoic acid can be minimum 1,2,3,4,5 or 6 months to the highest 1,2,3,4,5 year or even for more time, comprises the lifelong of animal.Alpha-lipoic acid plays a role in food, and it does not need any special protection, as encapsulating, and does not need to appear in food with the unit dosage forms such as such as tablet, pill, capsule used in medicine.The amount of lipoic acid in food that is provided in is at least about 25,50,75 or 100ppm of food.The highest scope, lower than its toxic level, is from start to finish low to moderate about 400,300 or 200ppm of food.Usually, daily dose can not exceed animal per kg body weight about 6 or 7mg, is more generally no more than about 5mg/kg.Alpha-lipoic acid improves the defence capability of antioxidant and improves the ability that animal resists oxidative damage.All these effects are all carry out under appropriate other antioxidant (as vitamin E and vitamin C) exists.This shows that the effect of alpha-lipoic acid is better than vitamin C and/or vitamin E.
Embodiment 2
Materials and methods:
The effect of lipoic acids is measured, with AAFCO control Food or test food containing fish oil and contrast with 30 dogs.
In AAFCO control Food, containing studying differential gene expression spectrum between fish oil food and the food of lipoic acid.On 30th, in minimum 1.3 times of changes place, compared with AAFCO control group, produce row 1212 gene, and with compared with fish oil food, produce row 1896 gene.
RNA extracts: use PAXgene RNA separating kit, be separated total RNA from whole blood sample.All measurements adopt dog 2Affymetrix genetic chip to carry out.For statistical analysis, by all measurement RMA standardization.All analyses are carried out with Partek.ANOVA t-inspection is carried out to the gene of differential expression between contrast and test food, and according to p-value cutoff 0.1, multiple change > +/-1.3 selects test food.
Gene expression: when compared with the dog of AAFCO control Food of feeding, finds that 1212 gene expressions change in the dog of feeding containing the test food of 150ppm lipoic acid.In addition, when compared with the test food containing fish oil, find in the dog of the test food of feeding lipoic acid, 1896 gene expressions change.The gene that table 7 shows divides into groups according to feeding control Food or containing those relevant expressive functions of fish oil food and direction.
Metabolite (Metabolomics): analysed for plasma metabolin, and compare and change relative to the multiple of the dog of feeding control Food.
Biomarker measures: adopt kit measurement serum cartilage markers based on ELISA to determine the concentration of cartilage markers.Using 0 earning in a day as analyze in covariant to regulate baseline value.
Result: compared with the similar food without lipoic acid, adds lipoic acid and the crosslinked end fragments of peptides of the c-terminus of II Collagen Type VI C-propetide and II Collagen Type VI can be made to reduce larger in food.These two labels known increase in osteoarthritis dog.In addition, the dog fat of feeding lipoic acid food reduces, and shows to utilize energy-producing fat (fat oxidation) to increase and reduce to produce glucose to keep more healthy body weight.In other words, the dog of feeding lipoic acid more effectively utilizes existing glucose and its energetic supersession is converted to fat metabolism for Energy harvesting.In addition, in blood plasma, metabolin hydroxyproline reduces, and it shows that cartilage destruction reduces, and to be that this metabolin is almost complete produced by cartilage reason.In addition, support this evidence by the change in the gene expression measured in serum white blood corpuscle, namely think to enhance and synthesize by cartilaginous element the protective effect of cartilage that increases and cause and reduce the expression of the enzyme of degraded cartilage.Finally, changes in gene expression shows propose high-fat utilization by increasing PDK4, and PDK4 suppresses glucose to form acetonate and metabolism is moved so that produce power to closedown acetyl-CoA, and upregulation of glucose transport albumen.Compared with the dog of feeding control Food, the dog of feeding lipoic acid also presents gene traits fine.
The display of this table is relative to the fat of lipoic acid treats and the difference of body weight and cartilage markers.
Table 4 illustrates to contrast with feeding and adds fish oil and contrast adds compared with the dog of lipoic acid, the change of the Body composition of the dog of feeding control Food.As shown in table 4, at the treatments period of 90 days, the dog that feeding contrast adds lipoic acid presented maximum changes of weight.
Table 5 illustrates to contrast with feeding and adds fish oil and contrast adds compared with the dog of lipoic acid, the change of the acetonate blood level of the dog of feeding control Food.As shown in table 5, at the treatments period of 30 days, the dog that feeding contrast adds lipoic acid presented maximum change in acetonate blood level.
ausing 0 earning in a day as the covariant in analysis
Table 6 illustrates within 30th, to contrast with feeding and adds fish oil and contrast adds compared with the dog of lipoic acid, and the dog after feeding control Food is classified as the percentage of gene traits obesity.As shown in table 6, at the treatment after date of 30 days, dissimilar with the dog of physical obesity on the dog gene that feeding contrast adds lipoic acid.
Table 7. and contrast or improve compared with food (improve contain fish oil), the gene relevant with energetic supersession to the cartilage that lipoic acid changes
Table 8 illustrates the composition in the present invention's exemplary pet food compositions.
Composition composition used prepared by table 8.
| ? | Composition |
| 1 | Wheat |
| 2 | Chinese sorghum (milo) |
| 3 | Corn |
| 4 | Chicken meal |
| 5 | Corn gluten meal |
| 6 | Poultry meal |
| 7 | Soybean oil |
| 8 | Linseed |
| 9 | Rice beer |
| 10 | Soy meal, 49% |
| 11 | Pal enhancer 1 |
| 12 | Megasse |
| 13 | Potassium citrate |
| 14 | Fish oil |
| 15 | DL-methionine |
| 16 | L-Lysine mono Hydrochloride |
| 17 | Salt |
| 18 | Calcium carbonate |
| 19 | Lipoic acid |
| 20 | Choline Chloride |
| 21 | Vitamin premix |
| 22 | L-threonine |
| 23 | Vitamin E |
| 24 | L-Trp |
| 25 | Lipoic acid |
| 26 | Mineral pre-mix |
| 27 | Anticorrisive agent |
Table 9 show dual-energy X-ray absorption apparatus (DEXA) measure feeding control Food on the 90th after dog, and compared with the dog of the pet food of feeding lipoic acid.DEXA is used to measure body fat more accurate than the body weight for assessment of health status.A dog can have a lot of muscle, but is judged as " overweight " through a lot of height/body weight chart.Vice versa can be that real-one dog may have a lot of fat and little muscle, and it is " fatty excess " but not overweight.
Table 10 illustrates the Body composition on 180th, to determine that the different pet food of feeding 5 kinds is after 180 days, and the dog result for the treatment of of relatively initial covariant.
Table 11 illustrates that contrast feeding comprises the dog of lipoic acid pet food on the 30th, the change of the metabolin measured in the blood sample of the dog of feeding control Food.As shown in table 11, the dog that feeding contrast adds lipoic acid presented the minimizing of kidney label after 30 days.
The present invention is not limited to the scope by specific embodiments disclosed in embodiment, and described embodiment is only considered as the exemplary illustration of some aspects of the present invention, and any functionally equivalent embodiment all comprises within the scope of the invention.Really, except those embodiments shown herein and describe, various amendment of the present invention is all apparent to those skilled in the art, and they all will fall in the scope of appended claim.
Quote herein or all patents of reference, patent application, publication and other bibliography incorporated herein by reference in allowed by law degree.The discussion of those bibliography is only for summarizing opinion wherein.Be not admit these patents any, patent application, publication or bibliography or its any part relevant to prior art of the present invention, and clearly retain the right that accuracy and the correlation of these patents, patent application, publication and other bibliography are queried.
Claims (18)
1. the purposes in composition prepared by the lipoic acid of effective dose or its salt, and described composition causes body fat loss after feeding in companion animals in need, and wherein said effective dose is that 100 ppm are to keeping the avirulent amount of pet.
2. the purposes of claim 1, wherein said effective dose is about 100 ppm to about 600 ppm.
3. the purposes of claim 1, wherein said effective dose is about 100 ppm to about 200 ppm.
4. the purposes of claim 1, wherein said companion animals is dog.
5. the purposes of claim 1, wherein said companion animals is cat.
6. the purposes of claim 1, the pet food compositions wherein comprising lipoic acid gives at least 15.
7. the purposes of claim 1, the pet food compositions wherein comprising lipoic acid gives at least 30.
8. the purposes of claim 1, the pet food compositions wherein comprising lipoic acid gives at least 45.
9. the purposes of claim 1, the pet food compositions wherein comprising lipoic acid gives every day.
10. the purposes in composition prepared by the lipoic acid of effective dose or its salt, and described composition increases cutability percentage after feeding in companion animals in need, and wherein said effective dose is that 100 ppm are to keeping the avirulent amount of pet.
The purposes of 11. claims 10, wherein said effective dose is about 100 ppm to about 600 ppm.
The purposes of 12. claims 10, wherein said effective dose is about 100 ppm to about 200 ppm.
The purposes of 13. claims 10, wherein said companion animals is dog.
The purposes of 14. claims 10, wherein said companion animals is cat.
The purposes of 15. claims 10, the pet food compositions wherein comprising lipoic acid gives at least 15.
The purposes of 16. claims 10, the pet food compositions wherein comprising lipoic acid gives at least 30.
The purposes of 17. claims 10, the pet food compositions wherein comprising lipoic acid gives at least 45.
The purposes of 18. claims 10, the pet food compositions wherein comprising lipoic acid gives every day.
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| EP (1) | EP2381796B1 (en) |
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| CN114025620A (en) * | 2019-01-18 | 2022-02-08 | 马斯公司 | Methods and compositions for treating intestinal dysbiosis |
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| AU2010276259B2 (en) * | 2009-07-21 | 2013-04-18 | Hill's Pet Nutrition, Inc. | Companion animal nutrition system |
| WO2012087511A1 (en) | 2010-12-23 | 2012-06-28 | Hill's Pet Nutrition, Inc. | Pet food compositions and methods for weight loss and maintenance |
| CA2822337C (en) | 2010-12-23 | 2016-10-04 | Hill's Pet Nutrition, Inc. | Pet food compositions and methods for treating arthritis and inflammation associated with arthritis |
| WO2018081175A1 (en) * | 2016-10-24 | 2018-05-03 | Habit, Llc | System and method for implementing meal selection based on vitals, genotype, and phenotype |
| WO2018118197A1 (en) * | 2016-12-21 | 2018-06-28 | Richard Postrel | Healthier aging in domesticated animals |
| CA3165404A1 (en) * | 2019-12-27 | 2021-07-01 | Hill's Pet Nutrition, Inc. | Pet food compositions |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999055331A1 (en) * | 1998-04-25 | 1999-11-04 | Skw Trostberg Aktiengesellschaft | Use of alpha lipoic acid to reduce appetite and/or body weight |
| US20020006907A1 (en) * | 2000-02-01 | 2002-01-17 | Paul Gardiner | Alpha lipoic acid based food supplement for increasing lean muscle mass and strength |
| US20030224061A1 (en) * | 2002-06-04 | 2003-12-04 | Pacioretty Linda M. | Oral composition with insulin-like activities and methods of use |
| WO2007063095A1 (en) * | 2005-11-30 | 2007-06-07 | Basf Aktiengesellschaft | Use of zinc salts of lipoic acid for treating fat metabolism disorders |
| JP2007308468A (en) * | 2006-05-18 | 2007-11-29 | Bhn Kk | Basal metabolism increasing agent |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001058271A1 (en) * | 2000-01-25 | 2001-08-16 | Juvenon, Inc. | Nutritional supplements for aged pets |
| US20020076470A1 (en) * | 2000-10-31 | 2002-06-20 | Colgate-Palmolive Company | Composition and method |
| WO2003097046A1 (en) * | 2002-05-17 | 2003-11-27 | Duke University | Method for treating obesity |
| DE602004013737D1 (en) * | 2003-03-12 | 2008-06-26 | Nestec Sa | PEPPER FOOD NUTRITION FOR DIET CHECK |
| CA2559974C (en) * | 2004-03-17 | 2012-08-14 | Nestec S.A. | Compositions and methods for reducing or preventing obesity |
| RU2394561C2 (en) * | 2004-11-24 | 2010-07-20 | Хилл`С Пет Ньютришн, Инк. | Methods of increasing immune response in animal |
| US8168161B2 (en) * | 2004-12-22 | 2012-05-01 | Hill's Pet Nutrition, Inc. | Method to promote oral health in companion animals |
| AU2005322887B2 (en) * | 2004-12-30 | 2010-03-11 | Hill's Pet Nutrition, Inc. | Methods for enhancing the quality of life of a senior animal |
| JP2006219467A (en) | 2005-02-10 | 2006-08-24 | Oriza Yuka Kk | Fat accumulation inhibitor |
| DK1906912T3 (en) * | 2005-07-14 | 2012-12-03 | Hills Pet Nutrition Inc | Method of Extending the Life of an Animal |
| EP1956923A1 (en) * | 2005-11-02 | 2008-08-20 | Nestec S.A. | Isoflavone compositions for reducing accumulation of body fat in male mammals, and methods for their use |
| JP4703388B2 (en) * | 2005-12-06 | 2011-06-15 | 植田製油株式会社 | Thioctic acid composition and method for producing the same |
-
2009
- 2009-12-29 CA CA2750137A patent/CA2750137C/en active Active
- 2009-12-29 WO PCT/US2009/069654 patent/WO2010078301A1/en active Application Filing
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- 2009-12-29 CN CN2009801538324A patent/CN102271530A/en active Pending
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999055331A1 (en) * | 1998-04-25 | 1999-11-04 | Skw Trostberg Aktiengesellschaft | Use of alpha lipoic acid to reduce appetite and/or body weight |
| US20020006907A1 (en) * | 2000-02-01 | 2002-01-17 | Paul Gardiner | Alpha lipoic acid based food supplement for increasing lean muscle mass and strength |
| US20030224061A1 (en) * | 2002-06-04 | 2003-12-04 | Pacioretty Linda M. | Oral composition with insulin-like activities and methods of use |
| WO2007063095A1 (en) * | 2005-11-30 | 2007-06-07 | Basf Aktiengesellschaft | Use of zinc salts of lipoic acid for treating fat metabolism disorders |
| JP2007308468A (en) * | 2006-05-18 | 2007-11-29 | Bhn Kk | Basal metabolism increasing agent |
Non-Patent Citations (2)
| Title |
|---|
| EUN YOUNG LEE,ETAL.: "Alpha-lipoic acid suppresses the development of collagen-induced arthritis and protects against bone destruction in mice", 《RHEUMATOL INT》 * |
| 赵康涛等: "60Co-γ射线照射大鼠损伤作用中硫辛酸对谷胱甘肽的调节与再生", 《第四军医大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114025620A (en) * | 2019-01-18 | 2022-02-08 | 马斯公司 | Methods and compositions for treating intestinal dysbiosis |
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| CA2750137C (en) | 2014-09-23 |
| BRPI0923783A2 (en) | 2015-07-21 |
| US20110269828A1 (en) | 2011-11-03 |
| EP2381796A1 (en) | 2011-11-02 |
| AU2009335166A1 (en) | 2011-06-23 |
| BRPI0923783A8 (en) | 2017-05-09 |
| CA2750137A1 (en) | 2010-07-08 |
| RU2482694C2 (en) | 2013-05-27 |
| EP2381796B1 (en) | 2019-02-20 |
| RU2011132017A (en) | 2013-02-10 |
| JP2012513764A (en) | 2012-06-21 |
| AU2009335166B2 (en) | 2012-12-13 |
| CN102271530A (en) | 2011-12-07 |
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