CN104215753B - A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof - Google Patents
A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof Download PDFInfo
- Publication number
- CN104215753B CN104215753B CN201410437748.8A CN201410437748A CN104215753B CN 104215753 B CN104215753 B CN 104215753B CN 201410437748 A CN201410437748 A CN 201410437748A CN 104215753 B CN104215753 B CN 104215753B
- Authority
- CN
- China
- Prior art keywords
- microsphere
- detectable
- lyophilizing
- reagent
- combining power
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof, comprise: the reagent such as buffer, carbonic anhydrase, phenol red, Bromothymol blue, preservative, trehalose, Polyethylene Glycol, TrionX-100。Its preparation process mainly has following steps: (1) preparation solution, filter, degassed；(2) liquid separation system utilizing accurate quantification prepares microdroplet, and microdroplet instills the freezing bead of preparation in liquid nitrogen；(3) being transferred in freeze dryer by freezing bead lyophilizing obtains freeze-dried reagent microsphere。CO2 combining power, CO2 CP detectable provided by the invention is a kind of form of spherical particles freeze-dried reagent, can be pre-packaged in detection chip, compare with existing liquid detection reagents and there is advantages such as can realizing ambient-temp-stable preservation, transport, with lyophilized powder Reagent evaluation ratio have quantitative accurately, be easy to the advantages such as encapsulation process, be applicable to the POCT biochemistry analyzer introducing microfluidic chip technology。
Description
Technical field
The present invention relates to lyophilizing microsphere and preparation field thereof, specifically a kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof。
Background technology
CO2 combining power, CO2 CP refers to the content of the carbon dioxide in blood plasma contained by bicarbonate radical。Respiratory acidosis: paralysis of respiratory muscle, bronchiectasis, pneumothorax and respiratory tract obstruction；Metabolic alkalosis: vomiting, adrenal cortex function is hyperfunction, potassium deficiency and take that alkalescent medicine is too much etc. all can cause acid alkali poisoning, so that CO2 combining power, CO2 CP increases；Metabolic acidosis, for instance uremia, shock, glycosuria ketosis, severe diarrhea and dehydration；Respiratory alkalosis, for instance respiratory center excitement and accelerated breathing etc. can cause CO2 combining power, CO2 CP to reduce。Therefore, measure the CO2 combining power, CO2 CP content in serum or blood plasma, be that fluid transport therapy corrects unbalance important indicator, treatment is significant。
Along with the continuous progress of society, medical test develops from large hospital to the care diagnostic field such as community, scene。Instant detection quickly grows, and instant detection can be used for the detection of in emergency circumstances various biochemical indicators, for instance on emergency room, emergency tender etc., numerous conditions are limited but still need to draw rapidly the scene application of testing result。The progress of microfluidic chip technology has been greatly facilitated the development of care diagnostic instrument。Microfluidic chip technology is integrated in the preparation of sample, separation, reaction and detection etc. on chip piece, microchannel form network, and then realize the accurate control of whole system fluid。Microfluidic chip technology is incorporated into POCT equipment, and the operation that conventional laboratory can be made complicated is automatically performed on chip, and Instrument structure is relatively easy, and volume is little, lightweight, simple to operate, is suitable for the needs of the other detection of bed, battlefield or small-sized outpatient service。
In 12 national strategy industrial development plannings, clearly propose to set up simple to operate, device is portable, measure instant detector device accurately。Existing CO2 combining power, CO2 CP liquid detection reagents not easily encapsulates and stores, and lyophilized powder reagent is not easily quantitative, is not all suitable for instant detector device, needs a kind of CO2 combining power, CO2 CP detectable that can be used for the instant detector device based on microflow control technique badly。
Summary of the invention
It is an object of the invention to provide a kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof, to solve prior art liquid and lyophilized powder CO2 combining power, CO2 CP detectable Problems existing。
In order to achieve the above object, the technical solution adopted in the present invention is:
A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: the weight proportion of its reaction reagent and buffer composition and each component is:
The weight proportion of other components such as the freeze drying protectant of this reagent lyophilizing microsphere and moulding dose of lyophilizing and each component is:
Described one concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: described reaction reagent selects buffer, it is preferable that Tris buffer, or phosphate buffer, or citric acid-trisodium citrate buffer。
Described one concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: described color source material is one or more in phenol red, Bromothymol blue。
Described one concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: described freeze drying protectant is saccharide, or polymer, or surfactant, wherein:
When freeze drying protectant is saccharide, it is preferable that sucrose, or trehalose, contents of saccharide is about 5g/L-30g/L；
When freeze drying protectant is polymer, it is preferable that Polyethylene Glycol, or polyvinylpyrrolidone, or bovine serum albumin, or glucosan, polymer content is about 50g/L-150g/L；
When freeze drying protectant is surfactant, it is preferable that Tween 80, or polysorbas20, or TrionX-100, or polyoxyethylene laurel ether, surface-active contents is about 1g/L-5g/L。
Described one concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: described plastotype agent is one or more in mannitol, Polyethylene Glycol, bovine serum albumin, PVP。
Described one concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: in described reagent reaction reagent and buffer composition is conventional reagent reaction reagent and buffer concentration 2-4 times。
A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing method for preparing microsphere, comprises the following steps:
(1), preparation CO2 combining power, CO2 CP detectable；
(2), adding freeze drying protectant and plastotype agent, be filtered to remove insoluble substance after fully dissolving, bubble is removed in vacuum outgas；
(3), utilize and the liquid separation system of accurate quantification can prepare microdroplet, drop is instilled in liquid nitrogen, after liquid nitrogen cools down, form freezing reagent microsphere；
(4), freezing reagent microsphere is proceeded to freeze dryer prepares after lyophilization reagent lyophilizing microsphere。
Described one concentration CO2 combining power, CO2 CP detectable lyophilizing method for preparing microsphere, it is characterised in that: the drop volume size in described step (3) utilizes liquid separation system accurately to control, and droplet size is at 2-10ul, and Size Error is in 2%。
The present invention has the following advantages and beneficial effect:
1, the CO2 combining power, CO2 CP detectable lyophilizing microsphere of the present invention, it is possible to achieve the stable preservation of room temperature, it is possible to effectively solving existing liquid CO 2 adhesion reagent cannot preserve by ambient-temp-stable, is not easily packaged in problem in care diagnostic instrument。
2, the CO2 combining power, CO2 CP detectable microsphere of the present invention, size and form is homogeneous, it is achieved that the accurate quantification of reagent, and the microsphere character adopted to make reagent take convenient, it is simple to encapsulation, solve existing freeze-dried powder reagent and not easily encapsulate quantitative problem。
3, the CO2 combining power, CO2 CP detectable microsphere of the present invention comprises the required reaction reagent of reaction and buffer, and all component is 2-3 times of concentration of normal reagent, only need to add sample during use and be dissolved to normal concentration, simplify operating procedure to greatest extent, be particularly well-suited to the care diagnostic instrument that reagent is pre-packaged。
Accompanying drawing explanation
Fig. 1 is the embodiment of the present invention 1, embodiment 2 CO2 combining power, CO2 CP detectable lyophilizing microsphere schematic diagram, wherein:
Fig. 1 a is embodiment 1 schematic diagram, and Fig. 1 b is embodiment 2 schematic diagram。
Fig. 2 is that the embodiment of the present invention 1, embodiment 2 CO2 combining power, CO2 CP detectable lyophilizing microsphere are with liquid reagent test result comparison diagram。
Detailed description of the invention
A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, the weight proportion of its reaction reagent and buffer composition and each component is:
The weight proportion of other components such as the freeze drying protectant of this reagent lyophilizing microsphere and moulding dose of lyophilizing and each component is:
Reaction reagent selects buffer, it is preferable that Tris buffer, or phosphate buffer, or citric acid-trisodium citrate buffer。
Color source material is one or more in phenol red, Bromothymol blue。
Freeze drying protectant is saccharide, or polymer, or surfactant, wherein:
When freeze drying protectant is saccharide, it is preferable that sucrose, or trehalose, contents of saccharide is about 5g/L-30g/L；
When freeze drying protectant is polymer, it is preferable that Polyethylene Glycol, or polyvinylpyrrolidone, or bovine serum albumin, or glucosan, polymer content is about 50g/L-150g/L；
When freeze drying protectant is surfactant, it is preferable that Tween 80, or polysorbas20, or TrionX-100, or polyoxyethylene laurel ether, surface-active contents is about 1g/L-5g/L。
Plastotype agent is one or more in mannitol, Polyethylene Glycol, bovine serum albumin, PVP。
In reagent reaction reagent and buffer composition is conventional reagent reaction reagent and buffer concentration 2-4 times。
A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing method for preparing microsphere, comprises the following steps:
(1), preparation CO2 combining power, CO2 CP detectable；
(2), adding freeze drying protectant and plastotype agent, be filtered to remove insoluble substance after fully dissolving, bubble is removed in vacuum outgas；
(3), utilize and the liquid separation system of accurate quantification can prepare microdroplet, drop is instilled in liquid nitrogen, after liquid nitrogen cools down, form freezing reagent microsphere；
(4), freezing reagent microsphere is proceeded to freeze dryer prepares after lyophilization reagent lyophilizing microsphere。
Drop volume size in step (3) utilizes liquid separation system accurately to control, and droplet size is at 2-10ul, and Size Error is in 2%。
The present invention can be used for the CO2 combining power, CO2 CP detectable lyophilizing microsphere of instant detector device and realizes by techniques below scheme: a kind of CO2 combining power, CO2 CP detectable lyophilizing microsphere that can be used for instant detector device; it is mainly composed of reaction reagent, buffer, freeze drying protectant, plastotype agent etc., specific as follows:
Its preparation method is as follows:
By component each in detectable according to mixing and stirring after formula proportion addition distilled water, preparing CO2 combining power, CO2 CP detectable, filter filters removes insoluble substance, and the bubble in solution is removed in vacuum outgas。Utilizing separatory pump to instill in liquid nitrogen by solution, freezing prepares frozen soln bead, proceeds in freeze dryer, and lyophilizing prepares reagent lyophilizing microsphere。
The each component of detectable solution of the present invention, surfactant be TrionX-100, polyoxyethylene laurel ether, Polyethylene Glycol, Tween 80 one or more；Preservative be methyl hydroxy-benzoate, ethylparaben, to the one in hydroxymethyl-benzoic acid propyl ester etc.。
Buffer in reaction reagent is the one in Tris buffer, phosphate buffer or citric acid-trisodium citrate buffer。
Freeze drying protectant freeze drying protectant in reaction reagent is saccharide, or polymer, or one or more in surfactant, wherein:
When freeze drying protectant is saccharide, it is preferable that sucrose, or trehalose, contents of saccharide is about 5g/L-30g/L；
When freeze drying protectant is polymer, it is preferable that Polyethylene Glycol, or polyvinylpyrrolidone, or bovine serum albumin, or glucosan, polymer content is about 50g/L-150g/L；
When freeze drying protectant is surfactant, it is preferable that Tween 80, or polysorbas20, or TrionX-100, or polyoxyethylene laurel ether, surface-active contents is about 1g/L-5g/L。
The each component of CO2 combining power, CO2 CP detectable is three times of conventional liq CO2 combining power, CO2 CP detectable component, and comprises reaction reagent and buffer in detectable, and the distilled water directly adding respective volume during use dissolves。
The reaction principle of CO2 combining power, CO2 CP detectable is: in sample, CO2 combining power, CO2 CP is under the effect of carbonic anhydrase, making bicarbonate radical decomposition move out with carbon dioxide causes pH value to change, develop the color by means of joint instructions agent, measure light absorption value at 546nm wavelength, calculate its content。
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this。
A kind of CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof, is mainly made up of following steps:
(1) preparation 3 times concentration CO2 combining power, CO2 CP detectable, its concrete formula is as follows:
Embodiment 1
Table 1, CO2 combining power, CO2 CP detectable form
| Composition | Concentration |
| Tris buffer | 90mM |
| Sodium chloride | 450mM |
| Carbonic anhydrase | 15KU/L |
| Phenol red | 420mg/L |
| Bromothymol blue | 180mg/L |
| Methyl hydroxy-benzoate | 1g/L |
| PEG 8000 | 26g/L |
| PEG 20000 | 22g/L |
| Trehalose | 15g/L |
| Surfactant Trion X-100 | 3g/L |
Embodiment 2
Table 2, CO2 combining power, CO2 CP detectable form
| Composition | Concentration |
| Tris buffer | 90mM |
| Sodium chloride | 450mM |
| Carbonic anhydrase | 15KU/L 5 --> |
| Phenol red | 420mg/L |
| Bromothymol blue | 180mg/L |
| Methyl hydroxy-benzoate | 1g/L |
| Macrogol 4000 | 25g/L |
| PEG 8000 | 22g/L |
| Sucrose | 15g/L |
| Surfactant Trion X-100 | 3g/L |
Surfactant TrionX-100 in CO2 combining power, CO2 CP detectable, it is possible to the bubble effectively controlled in reagent microsphere course of dissolution produces；Freeze drying protectant in reagent can adopt in sucrose, trehalose, Polyethylene Glycol, bovine serum albumin, polyvinylpyrrolidone, mannitol, glucosan one or more, and its content can be determined according to reagent lyophilizing microsphere form and dissolubility。
(2) by the CO2 combining power, CO2 CP detectable of above-mentioned concentration by the fluid liquid injection system of accurate measurement microdroplet can be prepared: the solution prepared is prepared microdroplet by fluid liquid injection system, droplet size can control according to demand, such as 2ul, 6ul, 10ul etc., being preferably every drop of liquid volume 6ul in the present embodiment, droplet size deviation is within 2%。
(3) drop is instilled in liquid nitrogen, after drop contacts with liquid nitrogen, the fast endothermic vaporization of liquid nitrogen local, form nitrogen stream and make droplets float on liquid nitrogen surface。After drop is fully cured, the freezing reagent bead formed sinks at the bottom of liquid nitrogen。Before liquid nitrogen is fully cured, the situation such as two drops can merge, adhesion, the present invention, by utilizing the spacing isolation methods such as porous plate, is effectively prevented drop adhesion and merges。
(4) will be equipped with the porous plate of freezing bead and be transferred in freeze dryer lyophilization, concrete dry run is 0.25mbar6h, 0.05mbar4h。
(5) after lyophilizing terminates, taking out reagent lyophilizing microsphere, dry enclosed package is in reagent bottle or detection chip。
The reagent lyophilizing microsphere of detection preparation, its performance is as follows:
1, lyophilizing microsphere size homogeneity, outward appearance etc., as it is shown in figure 1, the present embodiment obtain CO2 combining power, CO2 CP detectable lyophilizing microsphere smooth in appearance, size is homogeneous, dissolving is rapid, intensity is better, the reagent stability obtained after redissolution is better。
2, linear and detection sensitivity: with the CO2 combining power, CO2 CP detectable standard sample detectable lyophilizing microsphere performance of variable concentrations, and it is compared with liquid CO 2 adhesion detectable, such as Fig. 2:
From above-mentioned testing result, CO2 combining power, CO2 CP detectable lyophilizing microsphere size of the present invention is homogeneous, smooth in appearance, there is the close sensitivity of same liquid CO 2 adhesion detectable, the linearity and precision, the requirement in the fields such as care diagnostic can be met。
Claims (3)
1. a concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere, it is characterised in that: the weight proportion of its reaction reagent and buffer composition and each component is:
Tris buffer 30mM-180mM,
Sodium chloride 200mM-900mM,
Carbonic anhydrase 5KU/L-30KU/L,
Phenol red 100mg/L-600mg/L,
Bromothymol blue 45mg/L-360mg/L,
Preservative 1g/L-10g/L,
The weight proportion of the freeze drying protectant of this reagent lyophilizing microsphere and moulding dose of lyophilizing composition and each component is:
Macrogol 4000 10g/L-40g/L,
PEG 8000 10g/L-40g/L,
PEG 20000 20g/L-60g/L,
Trehalose 5g/L-30g/L,
TrionX-1001g/L-5g/L。
2. a concentration CO2 combining power, CO2 CP detectable lyophilizing method for preparing microsphere according to claim 1, it is characterised in that: comprise the following steps:
(1), preparation CO2 combining power, CO2 CP detectable；
(2), adding freeze drying protectant and plastotype agent, be filtered to remove insoluble substance after fully dissolving, bubble is removed in vacuum outgas；
(3), utilize and the liquid separation system of accurate quantification can prepare microdroplet, drop is instilled in liquid nitrogen, after liquid nitrogen cools down, form freezing reagent microsphere；
(4), freezing reagent microsphere is proceeded to freeze dryer prepares after lyophilization reagent lyophilizing microsphere。
3. a kind of concentration CO2 combining power, CO2 CP detectable lyophilizing method for preparing microsphere according to claim 2, it is characterized in that: the drop volume size in described step (3) utilizes liquid separation system accurately to control, droplet size is at 2-10 μ l, and Size Error is in 2%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410437748.8A CN104215753B (en) | 2014-08-30 | 2014-08-30 | A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410437748.8A CN104215753B (en) | 2014-08-30 | 2014-08-30 | A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104215753A CN104215753A (en) | 2014-12-17 |
| CN104215753B true CN104215753B (en) | 2016-06-22 |
Family
ID=52097466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410437748.8A Active CN104215753B (en) | 2014-08-30 | 2014-08-30 | A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104215753B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110923303A (en) * | 2019-11-27 | 2020-03-27 | 苏州天隆生物科技有限公司 | Universal freeze-drying protective agent for multiplex fluorescence PCR and application thereof |
| CN112684167A (en) * | 2020-12-18 | 2021-04-20 | 安邦（厦门）生物科技有限公司 | Chemiluminescent immunoassay reagent, freeze-dried microspheres and preparation method of freeze-dried microspheres |
| CN112980692B (en) * | 2021-03-09 | 2022-07-12 | 青岛高科技工业园海博生物技术有限公司 | Preparation and use method of quality control strain quantitative pellet |
| CN113281520A (en) * | 2021-04-26 | 2021-08-20 | 深圳市锦瑞生物科技有限公司 | Preparation method of reagent ball for determining total serum protein, reagent ball and microfluidic chip |
| CN113466151A (en) * | 2021-06-30 | 2021-10-01 | 重庆东渝中能实业有限公司 | Serum potassium detection reagent freeze-dried microsphere and preparation method thereof |
| CN113466150A (en) * | 2021-06-30 | 2021-10-01 | 重庆东渝中能实业有限公司 | Serum sodium detection reagent freeze-dried microsphere and preparation method thereof |
| CN114184452B (en) * | 2021-11-30 | 2024-03-01 | 阿里生物技术泰州有限公司 | Dye bead for centrifugal microfluidic disc chip and preparation method thereof |
| CN115541869B (en) * | 2022-11-28 | 2023-03-21 | 南京珀立科技有限公司 | Latex microsphere reagent freeze-drying process and application thereof in immunoturbidimetric reagent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102156126A (en) * | 2011-03-24 | 2011-08-17 | 董理 | Method and kit for detecting carbon dioxide bonding force |
| CN102830222A (en) * | 2012-09-20 | 2012-12-19 | 成都斯马特科技有限公司 | Method for preparing solid granular biochemical reagent |
| CN104198717A (en) * | 2014-08-30 | 2014-12-10 | 中国科学院苏州生物医学工程技术研究所 | Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2492488A1 (en) * | 2002-07-16 | 2004-01-22 | Elan Pharma International, Ltd. | Liquid dosage compositions of stable nanoparticulate active agents |
| WO2009052239A2 (en) * | 2007-10-19 | 2009-04-23 | Elc Management Llc | Methods and compositions for improving the appearance of keratinous surfaces |
-
2014
- 2014-08-30 CN CN201410437748.8A patent/CN104215753B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102156126A (en) * | 2011-03-24 | 2011-08-17 | 董理 | Method and kit for detecting carbon dioxide bonding force |
| CN102830222A (en) * | 2012-09-20 | 2012-12-19 | 成都斯马特科技有限公司 | Method for preparing solid granular biochemical reagent |
| CN104198717A (en) * | 2014-08-30 | 2014-12-10 | 中国科学院苏州生物医学工程技术研究所 | Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104215753A (en) | 2014-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104215753B (en) | A kind of concentration CO2 combining power, CO2 CP detectable lyophilizing microsphere and preparation method thereof | |
| CN104198717B (en) | A kind of concentrated glucose determination reagent freeze-drying microballoon and preparation method thereof | |
| US3466249A (en) | Blood serum reference standard for multi-automated analytical procedures | |
| CN107266563B (en) | Preparation method of hemoglobin control product | |
| CN103900884B (en) | Refining is combined quality-control product and preparation method thereof, test kit | |
| CN103917643A (en) | Devices containing dried reagents for reconstitution as calibration and/or quality control solutions, and methods of production and use thereof | |
| CN101543476A (en) | A naringin solid dispersion and the preparation method, and the application thereof | |
| CN103764028B (en) | Solidification controlling agent and the device comprising solidification controlling agent | |
| CN102688204B (en) | S-pantoprazole sodium freeze-drying medicament composition and preparation method thereof | |
| CN103520121B (en) | Vecuronium bromide for injection freeze drying powder injection and preparation method thereof | |
| CN103102512A (en) | Chitosan-fullerene compound and preparation method | |
| CN102212514A (en) | Blood coagulant for blood collection container | |
| CN114994218A (en) | Detection kit for detecting 4 fat-soluble vitamins in dried blood spots by liquid chromatography-tandem mass spectrometry and detection method thereof | |
| CN102657622A (en) | Preparation process of freeze-dry powder injection containing esomeprazole sodium | |
| CN113281520A (en) | Preparation method of reagent ball for determining total serum protein, reagent ball and microfluidic chip | |
| CN106546684B (en) | A kind of detection method of Alprostadil freeze-dried liplid emulsions | |
| CN104721138A (en) | Glabridin freeze-dried liposome and preparation method thereof | |
| CN104698159B (en) | A kind of detection method of endotoxin content | |
| WO2020023906A2 (en) | Polymer-based biospecimen collection devices and uses thereof | |
| CN102895189B (en) | Gingerol liposome and preparation method thereof | |
| CN103110656B (en) | Water-soluble vitamin freeze-dried preparation for injection and preparation method thereof | |
| CN103816126A (en) | Pharmaceutical composition containing scopolamine butylbromide | |
| CN102585198B (en) | A kind of not containing the polysorbate of Isosorbide acid anhydride polyoxyethylene fatty acid ester | |
| CN109632786A (en) | A method of accurately detecting phytosterol ester content using microplate reader | |
| CN113398079B (en) | Fludarabine freeze-dried powder for injection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |