CN104198549A - Method for determining content of cefoperazone sodium of cefoperazone sodium and tazobactam sodium for injection - Google Patents
Method for determining content of cefoperazone sodium of cefoperazone sodium and tazobactam sodium for injection Download PDFInfo
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- CN104198549A CN104198549A CN201410436068.4A CN201410436068A CN104198549A CN 104198549 A CN104198549 A CN 104198549A CN 201410436068 A CN201410436068 A CN 201410436068A CN 104198549 A CN104198549 A CN 104198549A
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- sodium
- tazobactam
- cefoperazone sodium
- cefoperazone
- damping fluid
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Abstract
The invention discloses a method for determining the content of cefoperazone sodium of cefoperazone sodium and tazobactam sodium for injection. A differential pulse voltammetry is adopted to scan the cefoperazone sodium and tazobactam sodium and can accurately quantify the cefoperazone sodium by the obtained peak current value. The method is simple and rapid and can achieve ideal sensitivity and accuracy.
Description
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Technical field
The present invention relates to medicine bioengineering field, particularly, relate to the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam.
Background technology
Cefoperazone is Third generation Cephalosporins microbiotic, reaches bactericidal action by the biosynthesizing that suppresses sensitive bacterial cell membrane.Tazobactam Sodium is except to Neisseriaceae and acinetobacter calcoaceticus, to other bacteriums without antibacterial activity, but Tazobactam Sodium has the inhibiting effect of nonreversibility to the important beta-lactamase of majority being produced by β-lactam antibiotics antibody-resistant bacterium.Tazobactam Sodium can prevent the destruction of drug-fast bacteria to penicillins and cephalosporins, and Tazobactam Sodium and penicillins and cephalosporins have obvious synergy.Because Tazobactam Sodium can combine with some PBP, stronger when therefore sensitive strain may be to the more alone cefoperazone of susceptibility of this compound preparation.
About all more complicated, troubles of assay method of the cefoperazone sodium content in Cefoperazone Sodium and Tazobactam, sensitivity and accuracy are not fine at present.
Summary of the invention
Technical matters to be solved by this invention is to provide the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam, the method adopts Differential Pulse Voltammetry to scan this Cefoperazone Sodium and Tazobactam, by gained peak point current, cefoperazone sodium is carried out to accurate quantitative analysis, the method is simple, quick, sensitivity and accuracy all more satisfactory.
The present invention addresses the above problem adopted technical scheme:
The cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam, comprises the following steps:
(1) preparation damping fluid, solution to be tested, cefoperazone sodium reference substance solution, sodium-tazobactam reference substance solution;
(2) get in the electrolytic cell that solution to be tested is positioned over electrochemical workstation, adopt three-electrode system, taking glass-carbon electrode as working electrode, saturated silver-silver chloride electrode is contrast electrode, and platinum electrode is auxiliary electrode, after enrichment 5min, static 6s, carry out differentiated pulse scanning at-0.6-1.5v, measure curve obtained with blank damping fluid and carry out background correction, measure the content of cefoperazone sodium.
Being formulated as of described damping fluid: take sodium dihydrogen phosphate 3.9g, be settled to 100mL with deionized water, storage at 4 DEG C.
When mensuration, damping fluid should return to room temperature and use.
Being formulated as of described cefoperazone sodium reference substance solution: take sodium-tazobactam 5mg, be placed in 100mL volumetric flask,, then measure 3mL and add that 4mL damping fluid is ultrasonic to be mixed and get final product to scale with deionized water constant volume.
Being formulated as of described sodium-tazobactam reference substance solution: take sodium-tazobactam 5mg, be placed in 25mL volumetric flask, be settled to scale with deionized water, then measure the aforementioned solution of 3mL and add that 4mL damping fluid is ultrasonic to be mixed and get final product.
To sum up, the invention has the beneficial effects as follows:
The present invention adopts Differential Pulse Voltammetry to scan this Cefoperazone Sodium and Tazobactam, by gained peak point current, cefoperazone sodium is carried out to accurate quantitative analysis, the method is simple, quick, sensitivity and accuracy all more satisfactory.
Embodiment
Below in conjunction with embodiment, the present invention is done to detailed description further, but embodiments of the present invention are not limited to this.
Embodiment 1:
The cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam, comprises the following steps:
(1) preparation damping fluid, solution to be tested, cefoperazone sodium reference substance solution, sodium-tazobactam reference substance solution;
(2) get in the electrolytic cell that solution to be tested is positioned over electrochemical workstation, adopt three-electrode system, taking glass-carbon electrode as working electrode, saturated silver-silver chloride electrode is contrast electrode, and platinum electrode is auxiliary electrode, after enrichment 5min, static 6s, carry out differentiated pulse scanning at-0.6-1.5v, measure curve obtained with blank damping fluid and carry out background correction, measure the content of cefoperazone sodium.
Being formulated as of described damping fluid: take sodium dihydrogen phosphate 3.9g, be settled to 100mL with deionized water, storage at 4 DEG C.
When mensuration, damping fluid should return to room temperature and use.
Being formulated as of described cefoperazone sodium reference substance solution: take sodium-tazobactam 5mg, be placed in 100mL volumetric flask,, then measure 3mL and add that 4mL damping fluid is ultrasonic to be mixed and get final product to scale with deionized water constant volume.
Being formulated as of described sodium-tazobactam reference substance solution: take sodium-tazobactam 5mg, be placed in 25mL volumetric flask, be settled to scale with deionized water, then measure the aforementioned solution of 3mL and add that 4mL damping fluid is ultrasonic to be mixed and get final product.
Claims (5)
1. the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam, is characterized in that, comprises the following steps:
(1) preparation damping fluid, solution to be tested, cefoperazone sodium reference substance solution, sodium-tazobactam reference substance solution;
(2) get in the electrolytic cell that solution to be tested is positioned over electrochemical workstation, adopt three-electrode system, taking glass-carbon electrode as working electrode, saturated silver-silver chloride electrode is contrast electrode, and platinum electrode is auxiliary electrode, after enrichment 5min, static 6s, carry out differentiated pulse scanning at-0.6-1.5v, measure curve obtained with blank damping fluid and carry out background correction, measure the content of cefoperazone sodium.
2. the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam according to claim 1, is characterized in that, being formulated as of described damping fluid: take sodium dihydrogen phosphate 3.9g, be settled to 100mL with deionized water, storage at 4 DEG C.
3. the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam according to claim 2, is characterized in that, when mensuration, damping fluid should return to room temperature and use.
4. the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam according to claim 1, it is characterized in that, being formulated as of described cefoperazone sodium reference substance solution: take sodium-tazobactam 5mg, be placed in 100mL volumetric flask,, then measure 3mL and add that 4mL damping fluid is ultrasonic to be mixed and get final product to scale with deionized water constant volume.
5. the cefoperazone sodium content assaying method of Cefoperazone Sodium and Tazobactam according to claim 1, it is characterized in that, being formulated as of described sodium-tazobactam reference substance solution: take sodium-tazobactam 5mg, be placed in 25mL volumetric flask, be settled to scale with deionized water, then measure the aforementioned solution of 3mL and add that 4mL damping fluid is ultrasonic to be mixed and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410436068.4A CN104198549A (en) | 2014-08-29 | 2014-08-29 | Method for determining content of cefoperazone sodium of cefoperazone sodium and tazobactam sodium for injection |
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| CN201410436068.4A CN104198549A (en) | 2014-08-29 | 2014-08-29 | Method for determining content of cefoperazone sodium of cefoperazone sodium and tazobactam sodium for injection |
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| CN104198549A true CN104198549A (en) | 2014-12-10 |
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| CN201410436068.4A Pending CN104198549A (en) | 2014-08-29 | 2014-08-29 | Method for determining content of cefoperazone sodium of cefoperazone sodium and tazobactam sodium for injection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111122683A (en) * | 2019-12-12 | 2020-05-08 | 同济大学 | Electrochemical method for detecting cephalosporin by three-dimensional interdigital printed electrode |
Citations (5)
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|---|---|---|---|---|
| CN1703619A (en) * | 2002-11-27 | 2005-11-30 | 法马西亚和厄普乔恩公司 | Methods of measuring the dissolution rate of an analyte in a non-aqueous liquid composition |
| CN1971258A (en) * | 2005-11-22 | 2007-05-30 | 中国药品生物制品检定所 | Method for evaluating stability of cefoperazone sodium leechdom |
| US20120245128A1 (en) * | 2011-03-25 | 2012-09-27 | The Board Of Regents Of The University Of Texas System | Rapid detection and quantification of modification of medicinal compounds and drug resistance activity |
| WO2012167218A2 (en) * | 2011-06-03 | 2012-12-06 | Dxupclose | Device and method for identifying microbes and counting microbes and determining antimicrobial sensitivity |
| CN103278587A (en) * | 2013-06-20 | 2013-09-04 | 中国环境科学研究院 | Method for separating and enriching three cephalosporin antibiotics in water environment |
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2014
- 2014-08-29 CN CN201410436068.4A patent/CN104198549A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703619A (en) * | 2002-11-27 | 2005-11-30 | 法马西亚和厄普乔恩公司 | Methods of measuring the dissolution rate of an analyte in a non-aqueous liquid composition |
| CN1971258A (en) * | 2005-11-22 | 2007-05-30 | 中国药品生物制品检定所 | Method for evaluating stability of cefoperazone sodium leechdom |
| US20120245128A1 (en) * | 2011-03-25 | 2012-09-27 | The Board Of Regents Of The University Of Texas System | Rapid detection and quantification of modification of medicinal compounds and drug resistance activity |
| WO2012167218A2 (en) * | 2011-06-03 | 2012-12-06 | Dxupclose | Device and method for identifying microbes and counting microbes and determining antimicrobial sensitivity |
| CN103278587A (en) * | 2013-06-20 | 2013-09-04 | 中国环境科学研究院 | Method for separating and enriching three cephalosporin antibiotics in water environment |
Non-Patent Citations (1)
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|---|
| 姜睿远 等: "微分脉冲伏安法测定注射用头孢哌酮钠他唑巴坦钠中头孢哌酮钠含量", 《中国实验方剂学杂志》, vol. 18, no. 15, 31 August 2012 (2012-08-31) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111122683A (en) * | 2019-12-12 | 2020-05-08 | 同济大学 | Electrochemical method for detecting cephalosporin by three-dimensional interdigital printed electrode |
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