CN104198478A - Rapid detection kit for metronidazole in cosmetics and detection method - Google Patents
Rapid detection kit for metronidazole in cosmetics and detection method Download PDFInfo
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- CN104198478A CN104198478A CN201410404073.7A CN201410404073A CN104198478A CN 104198478 A CN104198478 A CN 104198478A CN 201410404073 A CN201410404073 A CN 201410404073A CN 104198478 A CN104198478 A CN 104198478A
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Abstract
The invention relates to a rapid detection kit for metronidazole in cosmetics. The rapid detection kit is prepared from the following components: 10-20ml of acid solution, 10-20ml of alkaline solution, 5-10ml of an extraction agent, and 5-10ml of a catalyst. When the rapid detection kit is used for detecting the metronidazole in cosmetics, whether a sample contains metronidazole or not can be judged according to the color development reaction, the operation is simple, the detection time is short, the end point can be judged easily, special detection instruments and equipment are not required, and the kit can meet the rapid qualitative detection requirement of a cosmetic supervision department for the metronidazole in cosmetics.
Description
Technical field
The present invention relates to cosmetics detection field, be specifically related to quick detection kit and the detection method of metronidazole in cosmetics.
Background technology
In recent years, along with raising and the women of China's living standards of the people increase day by day to beautiful pursuit, bring up the flourish of the Cosmetic Market such as whitening, anti-acne, these cosmetics become indispensable necessity in people's life day by day, meanwhile, many illegal producers, in order to improve product efficacy, add forbidding or limit the use of material illegal without authorization, have caused serious potential safety hazard to the use of cosmetics.
Metronidazole is a kind of nitroimidazoles medicine, and chemical name is nitroimidazole-1,2-methyl-5 ethanol, and commodity metronidazole by name or Metronidazole, be wide spectrum anthelmintic, and anaerobion is all had to remarkable antibacterial effect, is the choice drug for the treatment of at present anaerobic infection.In addition, metronidazole can anti-itch mite, is that corynebacteria also has remarkable antibacterial action to the pathogen of acne, and therefore, current many producers often use it in cosmetics, especially in the product of anti-acne or the acne of dispelling.Although metronidazole is anti-acne or the acne of dispelling to a certain extent, prolonged application can produce significant stimulation to skin, causes erythema, oedema, the various skin problem such as rotten of rubbing.
Centers For Disease Control And Prevention In Beijing center environment clinic is to the regional anti-acne of selling such as Beijing, Jiangsu, except mite class cosmetics testing result shows, 11.5% product metronidazole exceeds standard, and maximum addition reaches 3.9%.In in August, 2007 China " cosmetics health specification ", propose first all kinds of antibiotic detections in these type of cosmetics.So far, the detection of cosmetics metronidazole comprises liquid phase chromatography, ELISA method, thin-layered chromatography, and these methods all can accurately detect metronidazole content in cosmetics, but operation recurrence, detection time is long, detects, cost is high, is not suitable for the quick large sample mobility-detected of cosmetics supervision department.The invention provides that a kind of detection method is easy, detection time is short, the method for quick that testing cost is low.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the invention provides that a kind of detection method is easy, detection time is short, the quick detection kit that testing cost is low and detection method.
Technical scheme of the present invention is as follows:
A quick detection kit for cosmetics metronidazole, described kit comprises following component:
Preferably, described acid solution is selected from H
2sO
4, one in HCL, acetic acid, the concentration of described acid solution is 0.5-0.8mol/L.
Preferably, described aqueous slkali is selected from the one in NaOH or KOH, and the concentration of described aqueous slkali is 1-5mol/L.
Preferably, described extractant is the aqueous solution of acetonitrile or the aqueous solution of methyl alcohol, the aqueous solution acetonitrile of described acetonitrile and the volume ratio of water are (5-9): (2-5), the aqueous solution methyl alcohol of described methyl alcohol and the volume ratio of water are (8--12): (2-5).
Preferably, described catalyzer is sulfuric acid or NaOH solution, the concentration 18-20mol/L of described sulfuric acid, and the concentration of described NaOH is 14-16mol/L
The method that adopts kit as above to detect metronidazole in cosmetics, comprises the following steps:
(1) sample preparation: take creme, cream or aqua sample 0.8-1.5g, be placed in 10ml color comparison tube, add extractant to scale, vortex shakes even, and ultrasonic extraction, obtains sample extracting solution;
(2) extract in step (1) is moved in the color comparison tube of another 10ml, add acid solution 2-5ml, shake up 5-10min, add aqueous slkali 2-5ml, add catalyzer, carry out chromogenic reaction, change judged result according to solution colour.
Metronidazole belongs to nitro glyoxaline compound, is easily reduced by alkali lye, generates mauve product, according to whether containing metronidazole in reaction solution result judgement sample, if reactant liquor is aubergine or pink colour in color comparison tube, can judges and in sample, contain metronidazole.
Compared to the prior art, beneficial effect of the present invention is:
1. the present invention adopts the simplest chemical reaction method to carry out qualitative detection to metronidazole in cosmetics, and method is easy, not high to equipment requirement, only needs conventional chemical degree agent can complete whole testing process.
2. the present invention adopts chemical reaction development process to whether containing metronidazole in cosmetics to qualitatively judge, and reactant liquor is inserted in color comparison tube, improves the accurate judgement to result, and terminal is easily judgement also.And before chromogenic reaction, first sample is carried out to pre-service, adopt organic solvent extraction and extract, further improve the accuracy of testing result.
In 3 the present invention, adopt catalyzer accelerated reaction, substitute traditional heating, do not need water bath heating device, operate easier.
Embodiment
Embodiment 1
A quick detection kit for cosmetics metronidazole, described kit comprises following component:
Described acid solution is H
2sO
4, concentration is 0.5mol/L.
Described aqueous slkali is NaOH, and concentration is 1mol/L.
Described extractant is the aqueous solution of acetonitrile, and the volume ratio of acetonitrile and water is 5: 2.
Described catalyzer is sulfuric acid, concentration 18mol/L.
The method that adopts kit as above to detect metronidazole in cosmetics, comprises the following steps:
(1) sample preparation: take creme sample 0.8g, be placed in 10ml color comparison tube, add extractant to scale, vortex shakes even, and ultrasonic extraction, obtains sample extracting solution;
(2) extract in step (1) is moved in the color comparison tube of another 10ml, add acid solution 2ml, shake up 5min, add aqueous slkali 2ml, add catalyzer, carry out chromogenic reaction, change judged result according to solution colour.
Reaction solution pinkiness, judges in this sample and contains metronidazole.
Embodiment 2
A quick detection kit for cosmetics metronidazole, described kit comprises following component:
Described acid solution is HCL, and concentration is 0.8mol/L.
Described aqueous slkali is NaOH, and concentration is 5mol/L.
Described extractant is the aqueous solution of methyl alcohol, and the volume ratio of methyl alcohol and water is 8: 2.
Described catalyzer is NaOH solution, and concentration is 14mol/L
The method that adopts kit as above to detect metronidazole in cosmetics, comprises the following steps:
(1) sample preparation: take cream sample 1.5g, be placed in 10ml color comparison tube, add extractant to scale, vortex shakes even, and ultrasonic extraction, obtains sample extracting solution;
(2) extract in step (1) is moved in the color comparison tube of another 10ml, add acid solution 5ml, shake up 10min, add aqueous slkali 5ml, add catalyzer, carry out chromogenic reaction, change judged result according to solution colour.
Reaction solution is aubergine, judges in this sample and contains metronidazole.
Embodiment 3
A quick detection kit for cosmetics metronidazole, described kit comprises following component:
Described acid solution is acetic acid, and concentration is for O.6mol/L.
Described aqueous slkali is NaOH, and concentration is 3.5mol/L.
Described extractant is the aqueous solution of acetonitrile, and the volume ratio of acetonitrile and water is 9: 5.
Described catalyzer is sulfuric acid, concentration 20mol/L.
The method that adopts kit as above to detect metronidazole in cosmetics, comprises the following steps:
(1) sample preparation: take aqua sample 1.2g, be placed in 10ml color comparison tube, add extractant to scale, vortex shakes even, and ultrasonic extraction, obtains sample extracting solution;
(2) extract in step (1) is moved in the color comparison tube of another 10ml, add acid solution 3.5ml, shake up 8min, add aqueous slkali 3.5ml, add catalyzer, carry out chromogenic reaction, change judged result according to solution colour.
It is colourless that reaction solution is, and judges and in this sample, do not contain metronidazole.
The above embodiment is preferred embodiments of the present invention, is not to limit the scope of the present invention, and the equivalence of doing according to structure, feature and principle described in the present patent application the scope of the claims therefore all changes or modifies, and all should comprise in patent claim of the present invention.
Claims (10)
1. a quick detection kit for cosmetics metronidazole, is characterized in that, described kit comprises following component:
Acid solution 10-20ml,
Aqueous slkali 10-20ml,
Extractant 5-10ml,
Catalyzer 5-10ml.
2. the quick detection kit of a kind of cosmetics metronidazole according to claim 1, is characterized in that, described acid solution is selected from H
2sO
4, one in HCL, acetic acid.
3. the quick detection kit of a kind of cosmetics metronidazole according to claim 1, is characterized in that, the concentration of described acid solution is 0.5-0.8mol/L.
4. the quick detection kit of a kind of cosmetics metronidazole according to claim 1, is characterized in that, described aqueous slkali is selected from the one in NaOH or KOH.
5. the quick detection kit of a kind of cosmetics metronidazole according to claim 1, is characterized in that, the concentration of described aqueous slkali is 1-5mol/L.
6. the quick detection kit of a kind of cosmetics metronidazole according to claim 1, is characterized in that, the aqueous solution that described extractant is acetonitrile or the aqueous solution of methyl alcohol.
7. the quick detection kit of a kind of cosmetics metronidazole according to claim 6, it is characterized in that, the aqueous solution acetonitrile of described acetonitrile and the volume ratio of water are (5-9): (2-5), the aqueous solution methyl alcohol of described methyl alcohol and the volume ratio of water are (8--12): (2-5).
8. the quick detection kit of a kind of cosmetics metronidazole according to claim 1, is characterized in that, described catalyzer is sulfuric acid or NaOH solution.
9. the quick detection kit of a kind of cosmetics metronidazole according to claim 8, is characterized in that, the concentration 18-20mol/L of described sulfuric acid, and the concentration of described NaOH is 14-16mol/L.
10. the method that the kit of employing as described in claim 1-5 detects metronidazole in cosmetics, is characterized in that, comprises the following steps:
(1) sample preparation: take creme, cream or aqua sample 0.8-1.5g, be placed in 10ml color comparison tube, add extractant to scale, vortex shakes even, and ultrasonic extraction, obtains sample extracting solution;
(2) extract in step (1) is moved in the color comparison tube of another 10ml, add acid solution 2-5ml, shake up 5-10min, add aqueous slkali 2-5ml, add catalyzer, carry out chromogenic reaction, change judged result according to solution colour.
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| CN201410404073.7A CN104198478A (en) | 2014-08-16 | 2014-08-16 | Rapid detection kit for metronidazole in cosmetics and detection method |
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| CN201410404073.7A CN104198478A (en) | 2014-08-16 | 2014-08-16 | Rapid detection kit for metronidazole in cosmetics and detection method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770229A (en) * | 2016-12-01 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | Metronidazole detection method and detection card in a kind of chicken |
| CN106770222A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection kit of metronidazole in a kind of food |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101498671A (en) * | 2009-03-12 | 2009-08-05 | 广东省药品检验所 | Fast screening method for metronidazole in cosmetic |
| CN101504372A (en) * | 2009-03-12 | 2009-08-12 | 广东省药品检验所 | Fast detection method and detection reagent kit for metronidazole, and its use in detection of cosmetic and disinfector added with metronidazole |
-
2014
- 2014-08-16 CN CN201410404073.7A patent/CN104198478A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101498671A (en) * | 2009-03-12 | 2009-08-05 | 广东省药品检验所 | Fast screening method for metronidazole in cosmetic |
| CN101504372A (en) * | 2009-03-12 | 2009-08-12 | 广东省药品检验所 | Fast detection method and detection reagent kit for metronidazole, and its use in detection of cosmetic and disinfector added with metronidazole |
Non-Patent Citations (3)
| Title |
|---|
| 李剑惠: "紫外分光光度法快速测定含漱液中甲硝唑的含量", 《安徽医药》 * |
| 王萍 等: "化妆品中磺胺及甲硝唑和氯霉素的测定方法及检出情况调查", 《中国卫生检验杂志》 * |
| 陆军 等: "高效液相色谱法同时测定祛痘产品中 6 种抗生素及甲硝唑", 《色谱》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770222A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection kit of metronidazole in a kind of food |
| CN106770229A (en) * | 2016-12-01 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | Metronidazole detection method and detection card in a kind of chicken |
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