CN104190699B - Method for screening soil organic pollution remediation plant - Google Patents
Method for screening soil organic pollution remediation plant Download PDFInfo
- Publication number
- CN104190699B CN104190699B CN201410419338.0A CN201410419338A CN104190699B CN 104190699 B CN104190699 B CN 104190699B CN 201410419338 A CN201410419338 A CN 201410419338A CN 104190699 B CN104190699 B CN 104190699B
- Authority
- CN
- China
- Prior art keywords
- plant
- organic
- extraction
- soil
- screening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides a method for screening a soil organic pollution remediation plant. The method comprises the following steps: calculating water content of the over-ground part of the plant; calculating a mass fraction of lipoid which is obtained by extracting an organic solvent by virtue of Soxhlet extraction in dry weight of the over-ground part of the plant; deducing a model formula about the potential of the plant of absorbing organic pollutants according to a restriction-distribution plant absorption model and the operations in the first two steps; and carrying out the operations in the first two steps on account of the over-ground parts of different plants, substituting the model formula and calculating the potentials of the over-ground parts of different plants of absorbing different target organic pollutants so as to screen the plant with the highest potential of absorbing the target organic pollutants as the soil organic pollution remediation plant. The method for screening the soil organic pollution remediation plant is simple and convenient to use and operate, wide in application range and capable of efficiently, stably, accurately and reliably screening the remediation plant, has high practical values and can be widely used for screening the soil organic pollution remediation plant.
Description
Technical field
The present invention relates to a kind of screening technique of soil organic contamination rehabilitation plant, belong to soil organic contamination reparation neck
Domain.
Background technology
Rapid development of economy causes great burden, after water pollution, atmosphere pollution, soil to ecological environment
The pollution problem of environment also increasingly highlights, and has clearly proposed to strengthen soil environment guarantor in national environmental protection " 12 " planning
Shield, and require to advance key area contaminated site and soil remediation, increase the investment of soil pollution repairing and treating.
The soil organic contamination that the administration of production activity, farmland Pesticides and insecticide in chemical plant etc. causes, has and holds
Long property, accumulative, and can human body be passed into by the food chain of crops, to human health generation serious harm.Compared to
Physics, the large-engineering amount of chemical repair method and high reparation spend, and take bioremediation technology to administer soil organic contamination obvious
Economical advantage, adds that biological prosthetic is environmentally friendly based technique for in-situ remediation, and bioremediation technology is therefore to repair
First-selection by organic polluted soil.Repair that potential is big, applied widely, the simple advantage of operation maintenance due to having, plant is repaiied
Recovering technology is just more and more being applied to the repairing and treating of soil organic contamination.And how to filter out can efficient absorption organic
The plant species of pollutant, are the bases of the smooth effectively enforcement of phytoremediation organic polluted soil.
At present, the screening technique of organic contamination rehabilitation plant mainly has two classes, and a class is directly to be measured by absorption experiment
The measurement method to organic pollution actual absorption ability for the plant, the selection result of this kind of method is reliable, but screening operation amount big,
Cycle length, efficiency are extremely inefficient, application extremely inconvenience；Another kind of screening technique is then (as plant group based on some plant parameters
Point, physiological activity index etc.) and organic pollution property (as hydrophobicity, molecular weight etc.) counted by plant absorption model formation
Calculate the model prediction that pre- measuring plants absorb potential to organic pollution, its advantage is the workload greatly reducing foliage filter screening
And the cycle, but differed due to the numerous and diverse degree of model, parameter detecting difficulty different, model prediction is difficult to take into account the effect of screening operation
The forthright reliability with the selection result, therefore it there is also certain restriction in the application of screening rehabilitation plant.
Restriction-distribution (Partition-limited) the plant absorption model being proposed by Chiou et al. is relatively simple
A kind of forecast model, this model propose plant absorption organic pollution drive force source from organic pollution in plant organic component
Between (wherein lipoid occupies absolute leading role) assigning process, its formula is：
Qeq=Cw×(flip×Klip+fch×Kch+fpw)≈Cw×(flip×Kow+fch×Kch+fpw)=Cw×flip×Kow
QeqThe absorption potential to organic pollution for the plant, mg/kg；
CwThe concentration of organic pollution in the soil liquid, mg/L, depending on soil property and pollution situation；
flip、fch、fpwIt is respectively cerolipoid, carbohydrate, the mass fraction of moisture, %；
Klip、KchIt is respectively distribution coefficient in cerolipoid, carbohydrate for the organic pollution, L/kg；
KowThe octanol-water partition coefficient of organic pollution, L/kg, is the Hydrophobic Parameters that organic matter is commonly used；
Plant depends on cerolipoid content to the absorption potential of organic pollution and the octanol-water of organic pollution is joined
COEFFICIENT Kow.Due to KowFor the basic physicochemical data of organic matter, easily consult acquisition, therefore it may only be necessary to by classical surname extraction
Method measures cerolipoid content, just can apply the absorption potential to organic pollution for the pre- measuring plants of this model formation, screens plant
Very convenient and efficient.But, due to underestimating to soxhlet extraction cerolipoid extraction efficiency, the screening side based on this model
Its judge error of method is larger, and accurately and reliably degree is poor.Deposit for solving existing organic contamination rehabilitation plant screening technique and technology
Deficiency or application limit, need to find a kind of accurately and reliably, convenient and efficient, rehabilitation plant screening side applied widely
Method, to ensure the popularization and application of organic polluted soil phytoremediation technology, promote soil organic contamination repair validity,
Environment friendly, economy.
Content of the invention
The technical problem to be solved in the present invention is to overcome existing defect, provides a kind of soil organic contamination rehabilitation plant
Screening technique, using simple and convenient, screening rehabilitation plant is efficient, stable, accurate, reliable, applied widely, has very high
Practical value, can be widely applied to the screening of organic contamination rehabilitation plant.
In order to solve above-mentioned technical problem, the invention provides following technical scheme：
A kind of screening technique of soil organic contamination rehabilitation plant, comprises the following steps：
A. after taking the aerial part 35～45g of screened plant to weigh fresh weight, vacuum freeze drying 31～41h, is planted
Thing dry sample, weighs the dry weight of this aboveground vegetation part immediately, is thus calculated the moisture content f of aboveground vegetation partpw；
B. with pulverizer, the plant obtaining in step a dry sample is milled to after powder, weighs 1～3g and load in filtration paper cylinder,
Record the gross weight of filtration paper cylinder simultaneously, then filtration paper cylinder is placed in the extraction tube of surname extraction bottle, in carrying of surname extraction bottle
Take after adding the organic extraction solvent of 95～105mL in pipe, with can the continuous apparatus,Soxhlet's of water-bath temperature control timing be planted
The extraction of species fat, bath temperature is set above 15～25 DEG C of organic extraction solvent boiling point used, continuous surname extraction when
Between be set as 7～17h, complete after lipoid extracts, organic solution containing cerolipoid in extraction flask poured into and weighed
In clean 250mL round-bottomed flask, using Rotary Evaporators by the organic extraction solvent evaporation in round-bottomed flask completely, subsequently claim
The weight of amount round-bottomed flask, the difference weighed twice as extracts the cerolipoid weight obtaining, further according to for surname extraction
Plant dry sample weight, just can be calculated and selected extract the lipoid that Solvent Extract methods obtain and account for aboveground vegetation part dry weight
Mass fraction flip(A)；
C. the operation being carried out according to restriction-distribution plant absorption model and step a, b, is derived from plant to organic dirt
The model formation of the absorption potential of dye thing,
QeqThe absorption potential to organic pollution for the aboveground vegetation part, mg/kg；
CwThe concentration of organic pollution in the soil liquid, mg/L, depending on soil property and pollution situation；
flip(A)The lipoid that selected extraction Solvent Extract methods obtain accounts for the mass fraction of aboveground vegetation part dry weight, %；
RAThe selected extraction recovery rate to cerolipoid for the organic solvent, %；
fpwThe moisture content of aboveground vegetation part, %；
KowThe octanol-water partition coefficient of organic pollution, L/kg, is the Hydrophobic Parameters that organic matter is commonly used；
D. the aerial part being directed to different plants carries out the operation of step a, b, and substitutes into the model formation in step c, just
The different absorption potential to different target organic pollution for the aboveground vegetation part of prediction can be calculated, thus filtering out organic to target
Pollutant has the plant of absorption maximum potential as soil organic contamination rehabilitation plant.
Further, the vacuum freeze drying time in described step a is 36h.
Further, the bath temperature in described step b is set above 20 DEG C of organic extraction solvent boiling point used.
Further, the time of the continuous surname extraction in described step b is set as 12h.
Further, the organic extraction solvent in described step b is volume ratio 3:The mixing of 1 acetone-chloroform is molten
Liquid, corresponding soxhlet extraction temperature is 80 DEG C, corresponding cerolipoid recovery rate RAFor 59.6%.
Further, screened plant is to grow the herbaceous plant that quick, biomass is big, can plant in many seasons.
Further, described target organic pollution is hydrophobicity in log KowNonionic in the range of >=2.8 is organic
Thing.
Beneficial effects of the present invention are：Model formation parameter is less and is readily available, using simple；Using method of operating letter
Just, and easily realize it is often more important that screening efficiency is high, be embodied in the mass of screening operation and the short cycle (can be in 3 days
Complete a collection of foliage filter screening, every batch of plant is several up to ten)；Core reagent, that is, organic extraction solvent be easily obtained, and price is low
Honest and clean；Accurately and reliably, method degree of accuracy assay shows the prediction of screening technique by mistake to the selection result being drawn by this screening technique
Difference (ratio absorbing potential and the plant absorption potential directly recording of model prediction) is within 20%；This screening technique be suitable for
Scope is wide, and rehabilitation plant that can be screened and organic pollution cover almost all of herbaceous plant and hydrophobicity respectively in log
KowNonionic organic matter in the range of >=2.8.
A kind of screening technique of present invention soil organic contamination rehabilitation plant, not only model formation and using simple to operate just
Victory, core reagent is cheap, be easily obtained, and when being applied to rehabilitation plant screening, efficient, stable, accurate, reliable, together
When applied widely, be a kind of very high rehabilitation plant rapid screening method of practical value, solve existing organic dirt well
The deficiency of dye rehabilitation plant screening technique and technology presence or application limit, and because of its outstanding practicality, reliability, can extensively answer
Screening for organic contamination rehabilitation plant.
Brief description
Accompanying drawing is used for providing a further understanding of the present invention, and constitutes a part for specification, the reality with the present invention
Apply example and be used for explaining the present invention together, be not construed as limiting the invention.In the accompanying drawings：
Fig. 1 is a kind of result of the application effect confirmatory experiment of screening technique of present invention soil organic contamination rehabilitation plant
Schematic diagram.
Specific embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are illustrated it will be appreciated that preferred reality described herein
Apply example to be merely to illustrate and explain the present invention, be not intended to limit the present invention.
Embodiment 1
A kind of screening technique of soil organic contamination rehabilitation plant, comprises the following steps：
A. after taking the aerial part 35～45g of screened plant to weigh fresh weight, vacuum freeze drying 31h, obtain plant and do
Sample, weighs the dry weight of this aboveground vegetation part immediately, is thus calculated the moisture content f of aboveground vegetation partpw；
B. with pulverizer, the plant obtaining in step a dry sample is milled to after powder, weighs 1～3g and load in filtration paper cylinder,
Record the gross weight of filtration paper cylinder simultaneously, then filtration paper cylinder is placed in the extraction tube of surname extraction bottle, in carrying of surname extraction bottle
Take after adding the organic extraction solvent of 95～105mL in pipe, with can the continuous apparatus,Soxhlet's of water-bath temperature control timing be planted
The extraction of species fat, bath temperature is set above 15 DEG C of organic extraction solvent boiling point used, and the time of continuous surname extraction sets
It is set to 7h, complete after lipoid extracts, the organic solution containing cerolipoid in extraction flask to be poured into weighed clean
In 250mL round-bottomed flask, using Rotary Evaporators by the organic extraction solvent evaporation in round-bottomed flask completely, subsequently weigh round bottom
The weight of flask, the difference weighed twice as extracts the cerolipoid weight obtaining, further according to the plant for surname extraction
Dry sample weight, just can be calculated selected extract the lipoid that Solvent Extract methods obtain and account for the quality of aboveground vegetation part dry weight divide
Number flip(A)；
C. the operation being carried out according to restriction-distribution plant absorption model and step a, b, is derived from plant to organic dirt
The model formation of the absorption potential of dye thing,
QeqThe absorption potential to organic pollution for the aboveground vegetation part, mg/kg；
CwThe concentration of organic pollution in the soil liquid, mg/L, depending on soil property and pollution situation；
flip(A)The lipoid that selected extraction Solvent Extract methods obtain accounts for the mass fraction of aboveground vegetation part dry weight, %；
RAThe selected extraction recovery rate to cerolipoid for the organic solvent, %；
fpwThe moisture content of aboveground vegetation part, %；
KowThe octanol-water partition coefficient of organic pollution, L/kg, is the Hydrophobic Parameters that organic matter is commonly used；
D. the aerial part being directed to different plants carries out the operation of step a, b, and substitutes into the model formation in step c, just
The different absorption potential to different target organic pollution for the aboveground vegetation part of prediction can be calculated, thus filtering out organic to target
Pollutant has the plant of absorption maximum potential as soil organic contamination rehabilitation plant.
Embodiment 2
Embodiment 2 is with the difference of embodiment 1, the screening technique of this soil organic contamination rehabilitation plant, the first two
Step is：
A. after taking the aerial part 35～45g of screened plant to weigh fresh weight, vacuum freeze drying 41h, obtain plant and do
Sample, weighs the dry weight of this aboveground vegetation part immediately, is thus calculated the moisture content f of aboveground vegetation partpw；
B. with pulverizer, the plant obtaining in step a dry sample is milled to after powder, weighs 1～3g and load in filtration paper cylinder,
Record the gross weight of filtration paper cylinder simultaneously, then filtration paper cylinder is placed in the extraction tube of surname extraction bottle, in carrying of surname extraction bottle
Take after adding the organic extraction solvent of 95～105mL in pipe, with can the continuous apparatus,Soxhlet's of water-bath temperature control timing be planted
The extraction of species fat, bath temperature is set above 25 DEG C of organic extraction solvent boiling point used, and the time of continuous surname extraction sets
It is set to 17h, complete after lipoid extracts, the organic solution containing cerolipoid in extraction flask to be poured into weighed clean
In 250mL round-bottomed flask, using Rotary Evaporators by the organic extraction solvent evaporation in round-bottomed flask completely, subsequently weigh round bottom
The weight of flask, the difference weighed twice as extracts the cerolipoid weight obtaining, further according to the plant for surname extraction
Dry sample weight, just can be calculated selected extract the lipoid that Solvent Extract methods obtain and account for the quality of aboveground vegetation part dry weight divide
Number flip(A)；
Embodiment 3
Embodiment 3 is with the difference of embodiment 1, the screening technique of this soil organic contamination rehabilitation plant, the first two
Step is：
A. after taking the aerial part 35～45g of screened plant to weigh fresh weight, vacuum freeze drying 36h, obtain plant and do
Sample, weighs the dry weight of this aboveground vegetation part immediately, is thus calculated the moisture content f of aboveground vegetation partpw；
B. with pulverizer, the plant obtaining in step a dry sample is milled to after powder, weighs 1～3g and load in filtration paper cylinder,
Record the gross weight of filtration paper cylinder simultaneously, then filtration paper cylinder is placed in the extraction tube of surname extraction bottle, in carrying of surname extraction bottle
Take after adding the organic extraction solvent of 95～105mL in pipe, with can the continuous apparatus,Soxhlet's of water-bath temperature control timing be planted
The extraction of species fat, bath temperature is set above 20 DEG C of organic extraction solvent boiling point used, and the time of continuous surname extraction sets
It is set to 12h, complete after lipoid extracts, the organic solution containing cerolipoid in extraction flask to be poured into weighed clean
In 250mL round-bottomed flask, using Rotary Evaporators by the organic extraction solvent evaporation in round-bottomed flask completely, subsequently weigh round bottom
The weight of flask, the difference weighed twice as extracts the cerolipoid weight obtaining, further according to the plant for surname extraction
Dry sample weight, just can be calculated selected extract the lipoid that Solvent Extract methods obtain and account for the quality of aboveground vegetation part dry weight divide
Number flip(A)；
In three above embodiment, the organic extraction solvent in described step b is volume ratio 3:1 acetone-three chloromethane
The mixed solution of alkane, corresponding soxhlet extraction temperature is 80 DEG C, corresponding cerolipoid recovery rate RAFor 59.6%；Screened plant
Thing is to grow the herbaceous plant that quick, biomass is big, can plant in many seasons；Described target organic pollution is hydrophobicity in log Kow
Nonionic organic matter in the range of >=2.8.
Below application effect confirmatory experiment is carried out to the screening technique of soil organic contamination rehabilitation plant proposed by the present invention：
Wheat and Lettuce aerial part (about 40g) are weighed after fresh weight, vacuum freeze drying 36h, weigh dry weight immediately,
Thus it is calculated the moisture content f of wheat and Lettuce aerial partpwIt is respectively 86.8%, 93.0%；With pulverizer by wheat
Be milled to after powder with Lettuce dry sample, weigh 2g about load in filtration paper cylinder (record weight), and filtration paper cylinder is placed in Soxhlet
In the extraction flask of surname extraction bottle, in the extraction tube of extraction flask, add the mixed solution (volume of 100mL acetone-chloroform
Ratio 3:1) after, carry out cerolipoid extraction with continuous apparatus,Soxhlet's, bath temperature is set to 80 DEG C, extraction time 12h；Complete
After lipoid extracts, the organic solvent in extraction flask is poured in the clean 250mL round-bottomed flask weighed, using rotary evaporation
Instrument by the organic solvent evaporation in round-bottomed flask completely, weighs the weight of round-bottomed flask, and the difference weighed twice is as extracted
The cerolipoid weight arriving, further according to the plant dry sample weight for surname extraction, is calculated wheat and Lettuce overground part
Divide the lipoid extracted amount f of (dry sample)lip(A)It is respectively 8.67%, 10.08%；Mixed solution (body due to acetone-chloroform
Amass and compare 3:1) lipoid recovery rate RAFor 59.6%, the luxuriant and rich with fragrance log K of selected target contaminantowFor 4.88 (i.e. KowFor
28840L/kg), therefore, it can calculate wheat aerial part to luxuriant and rich with fragrance absorption potential according to the model formation of screening technique
Qeq=554L/kg Cw, mg/kg；Lettuce aerial part is to luxuriant and rich with fragrance absorption Qeq=341L/kg Cw, mg/kg；Wherein, CwFor reality
The Sino-Philippines level of pollution (concentration) of border environment (soil liquid), mg/L.So far, obtained by the screening technique of the present invention
Wheat and Lettuce aerial part are to the luxuriant and rich with fragrance predicted value absorbing potential.The standard predicting the outcome for the screening technique verifying the present invention
Really property and reliability, to check its practical application effect, need to record wheat and Lettuce aerial part pair by adsorption equilibrium method
Luxuriant and rich with fragrance actual absorption potential, it comprises the following steps that：Compound concentration be respectively 0.1,0.2,0.4,0.6,0.8, the phenanthrene of 1.0mg/L
The aqueous solution (the CaCl containing 0.01M of (in water, solubility is 1.1mg/L, 25 DEG C)2), in 22mL and 40mL glass sample bottle with cover
Inside it is separately added into above-mentioned wheat and the Lettuce dry sample powder of 10 ± 0.5mg, 3 Duplicate Samples are set altogether；Then to glass sample
Bottle in be separately added into each concentration of 20mL and 40mL phenanthrene the aqueous solution, after screwing bottle cap, under the conditions of 25 DEG C, be placed on rotating disk with
The speed oscillation of 40rpm mixes 24 hours, makes absorption reach balance；After adsorption equilibrium, glass sample bottle is with the rotating speed of 3000rpm
Centrifugation 10min, and analyze the luxuriant and rich with fragrance concentration in supernatant with HPLC (high performance liquid chromatography), obtain luxuriant and rich with fragrance dense in the balance of aqueous phase
Degree；Because the Sino-Philippines dissipation of aqueous phase in adsorption process is only possible to be to be absorbed by wheat or Lettuce dry sample powder, therefore, it can root
Poor according to the initial of luxuriant and rich with fragrance solution and equilibrium concentration, and the liquor capacity of addition and wheat or Lettuce dry sample powder quality calculate
To equilibrium concentration in wheat or Lettuce dry sample powder for the phenanthrene, and thus try to achieve wheat or Lettuce dry sample powder to luxuriant and rich with fragrance suction
Attached thermoisopleth, and this is (C during certain level of pollutionw), wheat and Lettuce aerial part are to luxuriant and rich with fragrance actual absorption potential.By
This, the wheat that records of experiment and Lettuce aerial part are obtained with by the screening technique of the present invention to luxuriant and rich with fragrance actual absorption potential
Wheat and Lettuce aerial part the luxuriant and rich with fragrance predicted value absorbing potential is compared, to check the reality of screening technique of the present invention
Application effect (accuracy, reliability), result is as shown in Figure 1.From the graph, it is apparent that either wheat or naked oats
Dish, the aboveground vegetation part being obtained by the screening technique of the present invention is to the luxuriant and rich with fragrance predicted value absorbing potential, and is surveyed by adsorption experiment
The corresponding plant obtaining is very identical to luxuriant and rich with fragrance actual absorption potential, the absorption potential ratio that this screening technique is drawn by model prediction
Plant actual absorption potential deviation be less than 10% (deviation of wheat samples is -7.6%, the deviation of Lettuce sample is+
6.6%)；In addition, the sieve that same level of pollution, the selection result being drawn by this screening technique are drawn with actual adsorption experiment
Select result to be consistent, all show that wheat is the rehabilitation plant highly efficient to luxuriant and rich with fragrance pollution.
Measure the operating process that screening method uses by comparing the screening technique of the present invention and direct experiment in the present embodiment
Understand with the selection result drawing, the screening technique that the present invention provides not only reduces condition of work and the sieve of rehabilitation plant screening
Select workload (equipment requirement of screening and operation content), and the efficiency that improve screening (can complete a collection of plant in 3 days
Screening, every batch of plant is several up to ten；And direct experiment measures screening method and at least needs to complete for 5 days a collection of foliage filter screening, every batch
Plant is less than ten kinds)；Importantly, this screening technique show that the selection result accuracy is splendid, it is applied to different plant sieves
When selecting, the selection result is reliable and stable, possesses the screening effect that direct experiment measures screening method, the reality of screening technique of the present invention
Application effect (accuracy, reliability) is very good.
A kind of screening technique of present invention soil organic contamination rehabilitation plant, not only model formation and using simple to operate just
Victory, core reagent is cheap, be easily obtained, and when being applied to rehabilitation plant screening, efficient, stable, accurate, reliable, together
When applied widely, be a kind of very high rehabilitation plant rapid screening method of practical value, solve existing organic dirt well
The deficiency of dye rehabilitation plant screening technique and technology presence or application limit, and because of its outstanding practicality, reliability, can extensively answer
Screening for organic contamination rehabilitation plant.
Finally it should be noted that：The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention,
Although being described in detail to the present invention with reference to the foregoing embodiments, for a person skilled in the art, it still may be used
To modify to the technical scheme described in foregoing embodiments, or equivalent is carried out to wherein some technical characteristics.
All any modification, equivalent substitution and improvement within the spirit and principles in the present invention, made etc., should be included in the present invention's
Within protection domain.
Claims (7)
1. a kind of screening technique of soil organic contamination rehabilitation plant is it is characterised in that comprise the following steps：
A. after taking the aerial part 35～45g of screened plant to weigh fresh weight, vacuum freeze drying 31～41h, obtain plant and do
Sample, weighs the dry weight of this aboveground vegetation part immediately, is thus calculated the moisture content f of aboveground vegetation partpw；
B. with pulverizer, the plant obtaining in step a dry sample is milled to after powder, weighs 1～3g and load in filtration paper cylinder, simultaneously
Then filtration paper cylinder is placed in the extraction tube of surname extraction bottle, in the extraction tube of surname extraction bottle by the gross weight of record filtration paper cylinder
After the organic extraction solvent of interior addition 95～105mL, with the continuous apparatus,Soxhlet's of water-bath temperature control timing plant can be carried out
The extraction of fat, bath temperature is set above 15～25 DEG C of organic extraction solvent boiling point used, and the time of continuous surname extraction sets
It is set to 7～17h, complete after lipoid extracts, the organic solution containing cerolipoid in extraction flask to be poured into weighed clean
250mL round-bottomed flask in, using Rotary Evaporators by the organic extraction solvent evaporation in round-bottomed flask completely, subsequently weigh circle
The weight of bottom flask, the difference weighed twice as extracts the cerolipoid weight obtaining, further according to the plant for surname extraction
Thing dry sample weight, just can be calculated and selected extract the quality that the lipoid that Solvent Extract methods obtain accounts for aboveground vegetation part dry weight
Fraction flip(A)；
C. the operation being carried out according to restriction-distribution plant absorption model and step a, b, is derived from plant to organic pollution
Absorption potential model formation,
QeqThe absorption potential to organic pollution for the aboveground vegetation part, mg/kg；
CwThe concentration of organic pollution in the soil liquid, mg/L, depending on soil property and pollution situation；
flip(A)The lipoid that selected extraction Solvent Extract methods obtain accounts for the mass fraction of aboveground vegetation part dry weight, %；
RAThe selected extraction recovery rate to cerolipoid for the organic solvent, %；
fpwThe moisture content of aboveground vegetation part, %；
KowThe octanol-water partition coefficient of organic pollution, L/kg, is the Hydrophobic Parameters that organic matter is commonly used；
D. the aerial part being directed to different plants carries out the operation of step a, b, and substitutes into the model formation in step c, just can count
Calculate the different absorption potential to different target organic pollution for the aboveground vegetation part of prediction, thus filtering out to target organic contamination
Thing has the plant of absorption maximum potential as soil organic contamination rehabilitation plant.
2. a kind of soil organic contamination rehabilitation plant according to claim 1 screening technique it is characterised in that：Described step
The vacuum freeze drying time in rapid a is 36h.
3. a kind of soil organic contamination rehabilitation plant according to claim 1 screening technique it is characterised in that：Described step
Bath temperature in rapid b is set above 20 DEG C of organic extraction solvent boiling point used.
4. a kind of soil organic contamination rehabilitation plant according to claim 1 screening technique it is characterised in that：Described step
The time of the continuous surname extraction in rapid b is set as 12h.
5. a kind of soil organic contamination rehabilitation plant according to claim 1 screening technique it is characterised in that：Described step
Organic extraction solvent in rapid b is volume ratio 3:The mixed solution of 1 acetone-chloroform, corresponding soxhlet extraction temperature is
80 DEG C, corresponding cerolipoid recovery rate RAFor 59.6%.
6. a kind of soil organic contamination rehabilitation plant according to claim 1 screening technique it is characterised in that：Screened
Plant is to grow the herbaceous plant that quick, biomass is big, can plant in many seasons.
7. a kind of soil organic contamination rehabilitation plant according to claim 1 screening technique it is characterised in that：Described mesh
Indicating organic pollutants is hydrophobicity in log KowNonionic organic matter in the range of >=2.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410419338.0A CN104190699B (en) | 2014-08-22 | 2014-08-22 | Method for screening soil organic pollution remediation plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410419338.0A CN104190699B (en) | 2014-08-22 | 2014-08-22 | Method for screening soil organic pollution remediation plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104190699A CN104190699A (en) | 2014-12-10 |
| CN104190699B true CN104190699B (en) | 2017-02-08 |
Family
ID=52076146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410419338.0A Active CN104190699B (en) | 2014-08-22 | 2014-08-22 | Method for screening soil organic pollution remediation plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104190699B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105354624B (en) * | 2015-10-08 | 2019-08-30 | 江苏绿田环保科技有限公司 | A method of the efficiency of prediction phytoremediation organic polluted soil |
| CN109047304B (en) * | 2018-07-25 | 2021-08-10 | 西南科技大学 | Screening method and application of rhizomes plants for repairing soil uranium and cadmium combined pollution |
| CN112629966A (en) * | 2020-11-30 | 2021-04-09 | 中国科学院武汉岩土力学研究所 | Method for preparing uniform organic polluted soil |
| CN113578956B (en) * | 2021-08-02 | 2022-07-12 | 中国科学院地理科学与资源研究所 | Method and device for determining soil treatment plants |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3915930A1 (en) * | 1987-11-16 | 1990-11-22 | Chmiel Horst | Removing harmful substances from fluid - by passing fluid through polymer, lipoid or oil dispersed in medium, and removing with microorganisms |
| CN102513340A (en) * | 2011-12-13 | 2012-06-27 | 沈阳大学 | Method for repairing soils polluted by heavy metal Cd |
| CN103732319A (en) * | 2011-04-08 | 2014-04-16 | 萨里大学 | Oil absorbent composition |
| CN103894401A (en) * | 2014-04-22 | 2014-07-02 | 中国科学院地理科学与资源研究所 | Phytoremediation method for managing composite cadmium-lead-arsenic contaminated soil |
| CN103962368A (en) * | 2014-04-24 | 2014-08-06 | 常州大学 | Technical method for remedying cadmium-lead contaminated soil by animal-plant collaboration |
-
2014
- 2014-08-22 CN CN201410419338.0A patent/CN104190699B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3915930A1 (en) * | 1987-11-16 | 1990-11-22 | Chmiel Horst | Removing harmful substances from fluid - by passing fluid through polymer, lipoid or oil dispersed in medium, and removing with microorganisms |
| CN103732319A (en) * | 2011-04-08 | 2014-04-16 | 萨里大学 | Oil absorbent composition |
| CN102513340A (en) * | 2011-12-13 | 2012-06-27 | 沈阳大学 | Method for repairing soils polluted by heavy metal Cd |
| CN103894401A (en) * | 2014-04-22 | 2014-07-02 | 中国科学院地理科学与资源研究所 | Phytoremediation method for managing composite cadmium-lead-arsenic contaminated soil |
| CN103962368A (en) * | 2014-04-24 | 2014-08-06 | 常州大学 | Technical method for remedying cadmium-lead contaminated soil by animal-plant collaboration |
Non-Patent Citations (4)
| Title |
|---|
| A Partition-Limited Model for the Plant Uptake of Organic Contaminants from Soil and Water;Cary T. Chiou et.al;《Environ. Sci. Technol.》;20010302;第35卷(第7期);第1437–1444页 * |
| 有机污染植物修复模型中植物类脂的测定研究;王雪荣;《中国优秀硕士学位论文全文数据库》;20100215(第2期);全文 * |
| 植物类脂提取方法对吸附菲的影响研究;袁晋;《中国优秀硕士学位论文全文数据库》;20130315(第3期);第viii、5-8、12、14-17、32、34、35页 * |
| 油麦菜中类脂的测定及对菲的吸收预测;周红艺等;《浙江工业大学学报》;20100415;第38卷(第2期);第119-123页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104190699A (en) | 2014-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Köhne et al. | A review of model applications for structured soils: b) Pesticide transport | |
| Sprenger et al. | Established methods and new opportunities for pore water stable isotope analysis | |
| Orlowski et al. | Intercomparison of soil pore water extraction methods for stable isotope analysis | |
| Zhang et al. | Tritium analysis shows apple trees may be transpiring water several decades old | |
| Postigo et al. | Analysis and occurrence of selected medium to highly polar pesticides in groundwater of Catalonia (NE Spain): An approach based on on-line solid phase extraction–liquid chromatography–electrospray-tandem mass spectrometry detection | |
| CN104190699B (en) | Method for screening soil organic pollution remediation plant | |
| Xia et al. | Soil heavy metal concentrations and their typical input and output fluxes on the southern Song-nen Plain, Heilongjiang Province, China | |
| Bathurst et al. | Future of distributed modelling: the Système Hydrologique Européen | |
| CN104636627B (en) | A kind of heavy metal-polluted soil urban eco landscape forest method | |
| Wolfe et al. | Spectrofluorescence of sediment humic substances and historical changes of lacustrine organic matter provenance in response to atmospheric nutrient enrichment | |
| Correia et al. | Atrazine sorption and fate in a Ultisol from humid tropical Brazil | |
| Evrard et al. | Environmental DNA provides information on sediment sources: a study in catchments affected by Fukushima radioactive fallout | |
| Yoshida et al. | Concentrations of alkali and alkaline earth elements in mushrooms and plants collected in a Japanese pine forest, and their relationship with 137Cs | |
| Baran et al. | Hydrodynamic and geochemical constraints on pesticide concentrations in the groundwater of an agricultural catchment (Brévilles, France) | |
| CN204405652U (en) | A kind of native fish device detecting organism animal migration | |
| Mou et al. | Evaluating the methane generation rate constant (k value) of low-organic waste at Danish landfills | |
| Garcia et al. | Initial results of environmental monitoring in the Texas Rio Grande Valley | |
| CN107247083A (en) | A kind of heavy metals in farmland pollution on-line monitoring early warning and real time processing system and method | |
| Queloz et al. | Transport of fluorobenzoate tracers in a vegetated hydrologic control volume: 1. Experimental results | |
| CN107490559A (en) | A kind of method for determining oily area's solid waste petrochina class content | |
| Glendell et al. | Tracing of particulate organic C sources across the terrestrial-aquatic continuum, a case study at the catchment scale (Carminowe Creek, southwest England) | |
| Saber et al. | Rainfall-runoff simulation of radioactive cesium transport by using a small-scale portable rainfall simulator | |
| Raguž et al. | Plant uptake of elements in soil and pore water: Field observations versus model assumptions | |
| Dhiman et al. | Quantifying root water extraction after drought recovery using sub-mm in situ empirical data | |
| Wauchope et al. | Pesticides report 34. Pesticide runoff: Methods and interpretation of field studies (Technical Report) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |