CN104181317B - The biological detection paper instrument of a kind of portable multi-function and biological detection paper system, implementation method - Google Patents
The biological detection paper instrument of a kind of portable multi-function and biological detection paper system, implementation method Download PDFInfo
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- CN104181317B CN104181317B CN201410440633.4A CN201410440633A CN104181317B CN 104181317 B CN104181317 B CN 104181317B CN 201410440633 A CN201410440633 A CN 201410440633A CN 104181317 B CN104181317 B CN 104181317B
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- 238000001514 detection method Methods 0.000 title claims abstract description 34
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 23
- 239000003086 colorant Substances 0.000 claims abstract description 7
- 230000000638 stimulation Effects 0.000 claims abstract description 7
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 7
- 230000005669 field effect Effects 0.000 claims description 3
- 230000003993 interaction Effects 0.000 claims description 3
- 230000002687 intercalation Effects 0.000 claims description 3
- 238000009830 intercalation Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000003990 capacitor Substances 0.000 description 2
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Abstract
The invention discloses the biological detection paper instrument of a kind of portable multi-function, solve the problems such as existing detection paper instrument poor anti-interference, accuracy of detection are low.The biological detection paper instrument of this portable multi-function, comprising: a white light source, and reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source; Three filter plates, are respectively: ruddiness filter plate, green glow filter plate and blue light filter plate; Three light intensity freq converting circuits, and three light intensity freq converting circuits and ruddiness filter plate, green glow filter plate and blue light filter plate one_to_one corresponding; Microprocessor, is connected with three light intensity freq converting circuits, meanwhile, sends data to smart machine by USB data line.On the basis of said structure, the invention also discloses the biological detection paper system of portable multi-function and its implementation.
Description
Technical field
The present invention relates to a kind of detection paper equipment, specifically, relate to the biological detection paper instrument of a kind of portable multi-function and biological detection paper system, implementation method.
Background technology
Utilize detection paper content of material, it is one on-the-spot instant analysis method easily, be widely used because it is easy to carry, it is quick, simple to operate, cheap to test, its principle of work is: characteristic test paper and tested substance produce rapidly obvious color and change, namely the chemical reagent on test paper and measured matter reaction solution, the colour developing depth is relevant with measured matter content, and by measuring the depth of test paper color, measured matter content is measured out.Existing portable detection paper equipment, the Test paper limitednumber that its coupling uses, accuracy of detection is low.
Summary of the invention
The object of the invention is to overcome above-mentioned defect, provide the portable multi-function that a kind of structure is simple, cost is low, accuracy of detection is high biological detection paper instrument.
To achieve these goals, the technical solution used in the present invention is as follows:
The biological detection paper instrument of a kind of portable multi-function, is characterized in that, comprising:
A white light source, reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source;
Three filter plates, are respectively: ruddiness filter plate, green glow filter plate and blue light filter plate;
Three light intensity freq converting circuits, and three light intensity freq converting circuits and ruddiness filter plate, green glow filter plate and blue light filter plate one_to_one corresponding;
Microprocessor, is connected with three light intensity freq converting circuits, meanwhile, sends data to smart machine by USB data line.
Specifically, described light intensity freq converting circuit comprises a photodiode, two comparers, a RS latch, four field effect transistor; Wherein, the output terminal of RS latch connects microprocessor, and photodiode is for receiving the monochromatic light formed after filter plate filtering.Light intensity freq converting circuit is existing ripe circuit, and its role is to realize opto-electronic conversion, and therefore not to repeat here.
Preferential, described ruddiness filter plate, green glow filter plate and blue light filter plate set gradually along the length direction of reaction test paper.
On the basis of said structure, present invention also offers the biological detection paper system of a kind of portable multi-function, comprise reaction test paper, the biological detection paper instrument of a kind of portable multi-function, and be connected by the microprocessor in the biological detection paper instrument of USB data line and a kind of portable multi-function the smart mobile phone realizing data interaction.
The implementation method of the biological detection paper system of portable multi-function, comprises the following steps:
(1) white light source is opened, white light is injected ruddiness filter plate, produce fixed frequency square wave by light intensity freq converting circuit, microprocessor starts numeration function and the function that clocks simultaneously, adds up this light intensity freq converting circuit and produces time t1 required for 255 pulses;
(2) white light is injected respectively green glow filter plate and blue light filter plate, the light intensity freq converting circuit that microprocessor records green glow filter plate is corresponding produces time t2 required for 255 pulses, and the light intensity freq converting circuit that blue light filter plate is corresponding produces time t3 required for 255 pulses;
(3) intercalation reaction test paper, reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source;
(4) by the light intensity umber of pulse that freq converting circuit produces that interocclusal record ruddiness filter plate during t1 is corresponding;
(5) by the light intensity umber of pulse that freq converting circuit produces that interocclusal record green glow filter plate during t2 is corresponding; By the light intensity umber of pulse that freq converting circuit produces that interocclusal record blue light filter plate during t3 is corresponding;
(6) data in step (4) and step (5) are transferred to smart mobile phone by USB data line by microprocessor, and smart mobile phone calculates the value of ruddiness R, the value of green glow G and blue light B in RGB according to the data received;
(7) tabled look-up by rgb value and obtain reacting color corresponding to test paper.
Said method has used white balance method, eliminates the impact that light intensity freq converting circuit is different on three kinds of base light susceptibilitys, makes test result more accurate.
Compared with prior art, the present invention has following beneficial effect:
The present invention adopts white light source, reaction test paper is made to reflect coloured visible ray under the irradiation of white light source, coloured visible ray enters corresponding optical channel respectively after RGB three kinds of filter plate filtering, then turn to corresponding square-wave signal by light intensity conversion circuit wherein to export, finally identified by the frequency of microprocessor to square-wave signal of the part of data processing, then by USB interface, data are passed to smart mobile phone; Smart mobile phone calculates the value of RGB again according to the data received, thus identifies the reaction result of test paper; The present invention can test look test paper to the overwhelming majority on the market and make Accurate color identification, faint light signal is converted to frequency signal, compared with the equipment turning electricity with traditional light, its precision is higher, anti-interference stronger, thus make this portable set durable with stablizing, and have very high detection perform on the basis of low cost.
Accompanying drawing explanation
Fig. 1 is circuit theory diagrams of the present invention.
Fig. 2 is light intensity freq converting circuit figure in the present invention.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described, and embodiments of the present invention include but not limited to the following example.
Embodiment
As shown in Figure 1, present embodiments provide the biological detection paper instrument of a kind of portable multi-function, it comprises a white light source, three filter plates, three and filter plate light intensity freq converting circuit, microprocessor one to one.Wherein, the preferential LED white light source of white light source, reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source.Filter plate is respectively: ruddiness filter plate, green glow filter plate and blue light filter plate, and preferably, ruddiness filter plate, green glow filter plate and blue light filter plate set gradually along the length direction of reaction test paper.Reaction test paper reflects coloured visible ray under the irradiation of white light source, and coloured visible ray enters corresponding optical channel respectively after RGB three kinds of filter plate filtering.
Light intensity freq converting circuit, effect is light signal to be converted to electric signal, and coloured visible ray enters corresponding optical channel respectively after RGB three kinds of filter plate filtering, then turns to corresponding square-wave signal by light intensity conversion circuit wherein and exports.Light intensity freq converting circuit is existing ripe circuit, and as shown in Figure 2, in the present embodiment, light intensity freq converting circuit comprises a photodiode, two comparers, a RS latch, four field effect transistor; Wherein, the output terminal of RS latch connects microprocessor, and photodiode is for receiving the monochromatic light formed after filter plate filtering.Compared with the equipment turning electricity with traditional light, its precision is higher, anti-interference stronger.
Be described below in conjunction with the principle of work of accompanying drawing to light intensity freq converting circuit:
As shown in Figure 1, form monochromatic light exposure after (1) filtering on photodiode, at this moment diode produces fixing electric current and charges to electric capacity C1; (2) capacitance voltage increases gradually, when it is greater than the voltage of comparer A1 in-phase input end, and comparer A1 input low level; RS latch exports high level while latching level, closes P2 and N1 and opens P1 and N2 simultaneously, makes electric capacity C1 start electric discharge; (4) voltage of electric capacity C1 reduces gradually, when electric capacity C1 voltage is lower than comparer A2 in-phase input end voltage, and comparer A2 output low level; (5) RS latch exports zero setting, opens P2 and N1 while closing P1 and N2, and electric capacity C1 restarts charging, so just produces square wave at RS latch outputs.The charging current of side's wave frequency and photodiode is inversely proportional to.
The supply voltage V that three substitutional resistance R0 are corresponding is that the voltage of two comparer A1 and the input of A2 positive is respectively
with
charged from initial state by the known electric capacity of upper surface analysis
arrive
again because photodiode output current is linear, just can show that the duration of charging of electric capacity C1 is
Wherein i is diode charging voltage (its sizes values is directly proportional to light intensity), and C is the capacitance of electric capacity C1.
And when capacitor charging, RS latch exports as high level, so t
chargingalso the time exporting high level is equaled.
When the voltage of electric capacity is charged to
time, this circuit discharges until voltage is reduced to electric capacity
till.Its discharge time is
Wherein C is the capacitance of electric capacity C1.And when capacitor discharge, RS latch exports as low level, so t
electric dischargealso the time of output low level is equaled.
Just can know that output side's wave frequency of light intensity freq converting circuit is in sum
Have foregoing known, the square wave frequency that light intensity freq converting circuit produces is directly proportional to the electric current of photodiode, and the size of electric current is directly proportional to the intensity of light, so the square wave frequency that circuit exports is directly proportional to light intensity.The input current of photodiode is generally in μ A level, so the square wave frequency that the size of electric capacity can select pF to μ F to produce is Hz to KHz, the single-chip microcomputer of the very suitable low cost of such square wave frequency is measured.
Microprocessor, is mainly used in identifying the frequency of square-wave signal, and it also has numeration function and the function that clocks.
Based on said structure, the present embodiment also discloses the biological detection paper system of a kind of portable multi-function, comprise reaction test paper, the biological detection paper instrument of a kind of portable multi-function, and be connected by the microprocessor in the biological detection paper instrument of USB data line and a kind of portable multi-function the smart mobile phone realizing data interaction.
The course of work of the biological detection paper system of portable multi-function is as follows: (1) opens white light source, white light is injected ruddiness filter plate, fixed frequency square wave is produced by light intensity freq converting circuit, microprocessor starts numeration function and the function that clocks simultaneously, adds up this light intensity freq converting circuit and produces time t1 required for 255 pulses; (2) white light is injected respectively green glow filter plate and blue light filter plate, the light intensity freq converting circuit that microprocessor records green glow filter plate is corresponding produces time t2 required for 255 pulses, and the light intensity freq converting circuit that blue light filter plate is corresponding produces time t3 required for 255 pulses; (3) intercalation reaction test paper, reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source; (4) by the light intensity umber of pulse that freq converting circuit produces that interocclusal record ruddiness filter plate during t1 is corresponding; (5) by the light intensity umber of pulse that freq converting circuit produces that interocclusal record green glow filter plate during t2 is corresponding; By the light intensity umber of pulse that freq converting circuit produces that interocclusal record blue light filter plate during t3 is corresponding; (6) data in step (4) and step (5) are transferred to smart mobile phone by USB data line by microprocessor, and smart mobile phone calculates the value of ruddiness R, the value of green glow G and blue light B in RGB according to the data received; (7) tabled look-up by rgb value and obtain reacting color corresponding to test paper.The method has used white balance method, eliminates the impact that light intensity freq converting circuit is different on three kinds of base light susceptibilitys, makes test result more accurate.
According to above-described embodiment, just the present invention can be realized well.What deserves to be explained is; under prerequisite based on above-mentioned design concept; for solving same technical matters; even if some making on architecture basics disclosed in this invention are without substantial change or polishing; the essence of the technical scheme adopted is still the same with the present invention, therefore it also should in protection scope of the present invention.
Claims (4)
1. the biological detection paper instrument of portable multi-function, is characterized in that, comprising:
A white light source, reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source;
Three filter plates, are respectively: ruddiness filter plate, green glow filter plate and blue light filter plate;
Three light intensity freq converting circuits, and three light intensity freq converting circuits and ruddiness filter plate, green glow filter plate and blue light filter plate one_to_one corresponding;
Microprocessor, is connected with three light intensity freq converting circuits, meanwhile, sends data to smart machine by USB data line; Described light intensity freq converting circuit comprises a photodiode, two comparers, a RS latch, four field effect transistor; Wherein, the output terminal of RS latch connects microprocessor, and photodiode is for receiving the monochromatic light formed after filter plate filtering.
2. the biological detection paper instrument of a kind of portable multi-function according to claim 1, it is characterized in that, described ruddiness filter plate, green glow filter plate and blue light filter plate set gradually along the length direction of reaction test paper.
3. the biological detection paper system of portable multi-function, it is characterized in that, comprise reaction test paper, the biological detection paper instrument of a kind of portable multi-function as described in any one of claim 1 to 2, and be connected by the microprocessor in the biological detection paper instrument of USB data line and a kind of portable multi-function the smart mobile phone realizing data interaction.
4. the implementation method of the biological detection paper system of portable multi-function as claimed in claim 3, is characterized in that, comprise the following steps:
(1) open white light source, white light is injected ruddiness filter plate, produce fixed frequency square wave by light intensity freq converting circuit, microprocessor starts numeration function and the function that clocks simultaneously, adds up this light intensity freq converting circuit and produces the time required for 255 pulses;
(2) white light is injected respectively green glow filter plate and blue light filter plate, the light intensity freq converting circuit that microprocessor records green glow filter plate is corresponding produces the time required for 255 pulses, and the light intensity freq converting circuit that blue light filter plate is corresponding produces the time required for 255 pulses;
(3) intercalation reaction test paper, reaction test paper, after reacting with measured object, reflects the light of different colors under the stimulation of white light source;
(4) the light intensity umber of pulse that freq converting circuit produces that used time interocclusal record ruddiness filter plate is corresponding;
(5) the light intensity umber of pulse that freq converting circuit produces that used time interocclusal record green glow filter plate is corresponding; The light intensity umber of pulse that freq converting circuit produces that used time interocclusal record blue light filter plate is corresponding;
(6) data in step (4) and step (5) are transferred to smart mobile phone by USB data line by microprocessor, and smart mobile phone calculates the value of ruddiness R, the value of green glow G and blue light B in RGB according to the data received;
(7) tabled look-up by rgb value and obtain reacting color corresponding to test paper.
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| CN106706609B (en) * | 2015-08-18 | 2021-03-26 | 中国科学院宁波材料技术与工程研究所 | Method for rapidly detecting metal ions on site based on smart phone |
| CN108489977A (en) * | 2018-03-27 | 2018-09-04 | 张治国 | A kind of multi-functional medicine detection module and its detection method |
| CN109856133B (en) * | 2019-01-29 | 2021-06-22 | 深圳市象形字科技股份有限公司 | Test paper detection method utilizing multiple illumination intensities and multiple color illumination |
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| HUP9800290A3 (en) * | 1998-02-11 | 2000-03-28 | 77 Elektronika Mueszeripari Kf | Device for automatically analyzing a test stripe |
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| EP0286101A2 (en) * | 1987-04-07 | 1988-10-12 | Kabushiki Kaisha Kyoto Daiichi Kagaku | A method for analyzing special component in liquid |
| EP0594108A1 (en) * | 1992-10-19 | 1994-04-27 | Hitachi, Ltd. | liquid sample automatic analyzer |
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