CN104163499A - Application of Chelatococcus daeguensis in wastewater treatment - Google Patents
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- 241000439780 Chelatococcus daeguensis Species 0.000 title claims abstract description 16
- 238000004065 wastewater treatment Methods 0.000 title claims abstract description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 61
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 33
- 101150016368 TAD1 gene Proteins 0.000 claims abstract description 25
- 239000002351 wastewater Substances 0.000 claims abstract description 24
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000001360 synchronised effect Effects 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 41
- 230000001580 bacterial effect Effects 0.000 claims description 27
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 21
- 241000040710 Chela Species 0.000 claims description 19
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 6
- 235000013379 molasses Nutrition 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 229960004249 sodium acetate Drugs 0.000 claims description 4
- 239000001509 sodium citrate Substances 0.000 claims description 4
- 229940074404 sodium succinate Drugs 0.000 claims description 4
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 claims description 4
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 4
- 229940038773 trisodium citrate Drugs 0.000 claims description 4
- 238000009713 electroplating Methods 0.000 claims description 3
- 239000010813 municipal solid waste Substances 0.000 claims description 3
- 239000011651 chromium Substances 0.000 abstract description 68
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 23
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 231100000419 toxicity Toxicity 0.000 abstract description 3
- 230000001988 toxicity Effects 0.000 abstract description 3
- 238000005067 remediation Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 21
- 230000008569 process Effects 0.000 description 18
- 229910052804 chromium Inorganic materials 0.000 description 15
- 238000012258 culturing Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000009467 reduction Effects 0.000 description 7
- 229910002651 NO3 Inorganic materials 0.000 description 6
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 6
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical group [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 3
- 241000270666 Testudines Species 0.000 description 3
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 239000004912 1,5-cyclooctadiene Substances 0.000 description 1
- 241000738299 Chelatococcus sp. Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 125000002837 carbocyclic group Chemical group 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
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- 235000011187 glycerol Nutrition 0.000 description 1
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- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 238000011392 neighbor-joining method Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 239000001272 nitrous oxide Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
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Abstract
The invention discloses an application of Chelatococcus daeguensis in wastewater treatment. The strain is Chelatococcus daeguensis TAD1 with the collection number of CGMCC NO.5226. The Chelatococcus daeguensis CGMCC 5226 is used for synchronously removing nitrogen and hexavalent chromium in wastewater. The strain has broad spectrum activity in adaptability to temperature and has high removal efficiency at 30-50 DEGC. With the strain, Cr (VI) is reduced to Cr (III) with little toxicity, and part of nitrogen is reduced to N2 or N2O. Thus, the strain can be used to realize synchronous removal of nitrogen, Cr (VI) and carbon, and has a wide application prospect in polluted water body remediation.
Description
Technical field
The present invention relates to strain chela billiard ball bacterium (Chelatococcus daeguensis) TAD1 and remove the application in Cr (VI) at wastewater biological denitrificaion.This bacterial strain can be brought into play aerobic denitrification denitrification functions under different condition, and in denitrogenation, completes the removal to Cr (VI), thereby reaches waste water simultaneous denitrification except chromic object.
Background technology
Along with the increase of people to water demand, and the aggravation of water pollution problems, the water resources crisis difficult problem that becomes international.Water resources in china total reserves, the rank world the 4th, water resources ownership per capita is only 1/4 of world average level, 110 of ranks are one of 13 poor-water countries in the world.Under the sedimentation of industrial gaseous waste oxynitride, the migration of solid waste, ooze, the excessive of the unreasonable discharge of waste water, nitrogenous fertilizer use and the factors such as reuse irrigation of sewage, causes water body nitrate seriously polluted.Ministry of Land and Resources's statistics discovery, China's each department underground water has azotate pollution situation to occur, and nitrate has become the general factor that water pollutes.Nitrate easily changes into nitrite in human body, and nitrite can cause canceration, the distortion of human organ, extremely harmful to HUMAN HEALTH.Hexavalent chromium compound is a kind of common industrial raw material, is widely used in the industries such as plating, printing and dyeing, process hides.The random heap of the discharge of chromate waste water, chromium slag is abandoned, the sedimentation of dust, all causes the hexavalent chromium concentration in environment to exceed normal range.Sexavalent chrome is the carcinogenic metal of generally acknowledging, especially can cause that canceration occurs for nose, lung.The foremost chromium public hazard incident of China is that Qujing of Yunnan pollution of chromium event, due to arbitrarily dumping of chromium slag, makes water body hexavalent chromium concentration surpass 242 times of normal values, to local people, causes huge property damage and health hazards.
Since the eighties in last century, the reports such as Robertson Lesley have been found aerobic denitrification enzyme, and increasing investigator has confirmed the existence of Aerobic Denitrification Phenomenon, and the separated many strains aerobic denitrifying bacteria that obtains of success.Compare other denitride technologies, aerobic denitrification has fast, environmental protection, low consumption, the advantage such as efficient, is the study hotspot of current denitride technology.The seventies in last century, strain Cr (VI) restoring function bacterium separation in anaerobic environment obtains, and biological reducing is considered to the potential Cr of tool (VI) recovery technique.In polluted-water, nitrate and the heavy metal phenomenon ubiquity that coexists, obtains a kind of method of simultaneously removing these two kinds of pollution substances and seems very important.Domestic scholar, is to inquire into heavy metal to impact the interference effect to aerobic denitrification denitrogenation mostly, or utilizes aerobic sludge granular Adsorption heavy metal, for the bioremediation Technology of removing two pollutants simultaneously, rarely has report.Modification Zero-valent Iron is repaired nitrate and heavy metal-polluted water, although the method is more ripe, input cost is higher and easily cause secondary pollution.The with serious pollution water body of Cr (VI), as leather-making waste water, garbage leachate, people have all found the existence of Aerobic Denitrification Phenomenon, make to utilize biological process synchronously to remove these two kinds of pollutents possibility is provided.Bacterial strain in the present invention can be brought into play aerobic denitrification denitrification functions under different dissolved oxygen conditions, and concrete summary of the invention has been applied for patent of invention (application publication number CN102373169A); By to this bacterium the further research in sewage treatment process find, this bacterium shows stronger Cr (VI) removal ability in denitrogenation, at biological denitrificaion, is significant on except chromic theory and practice application.
Summary of the invention
The object of the present invention is to provide the strain can simultaneous denitrification and except chela billiard ball bacterium (Chelatococcus daeguensis) and the application thereof of Cr (VI) function, solved well water body nitrogen and sexavalent chrome combined pollution problem.
Bacterial strain provided by the invention has following characteristics:
(1) colony characteristics: bacterium colony is that rule is circular, and smooth surface is moistening transparent, is faint yellow; (2) cell morphological characteristic: cell becomes rod-short, size is (0.67～0.89) μ m * (1.03～1.41) μ m, peritrichous, Gram-negative; (3) the 16S rDNA gene sequence characteristic of huge legendary turtle billiard ball bacterium (Chelatococcus daeguensis) TAD1: its 16S rDNA has the nucleotide sequence shown in sequence table, fragment length is 1385bp, accession number in GenBank is HM000004, compare and show with GenBank database, the homology of this bacterial strain and huge legendary turtle billiard ball bacterium (Chelatococcus daeguensis) is 99%, wherein reaches 99% to the most similar homology of type strain Chelatococcus daeguensis strain K106.Application MEGA software adopts Neighbor-Joining method to draw 16S rDNA phylogenetic tree, determine its evolutionary degree, in conjunction with its morphological specificity and physiological and biochemical property, this bacterial strain is most possibly chela billiard ball Pseudomonas (Chelatococcus sp.), and called after Chelatococcus daeguensis strain TAD1.
Huge legendary turtle billiard ball bacterium of the present invention (Chelatococcus daeguensis) TAD1, by the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, it is referred to as CGMCC, its deposit number is CGMCC No.5226, preservation date is on September 6th, 2011, in the patent that its preservation proof has been CN102373169 at notification number, submits to.
It is sole carbon source that the chela billiard ball bacterium CGMCC5226 providing as above-mentioned can utilize sodium succinate, sodium acetate, Trisodium Citrate, waste molasses etc., and nitric nitrogen or ammonia nitrogen are that only nitrogen source carries out growth metabolism and brings into play aerobic denitrification, thereby removes nitrogen.It is sole carbon source that this bacterial strain can utilize sodium succinate, sodium acetate, Trisodium Citrate, waste molasses etc., nitric nitrogen or ammonia nitrogen are the sexavalent chrome that only nitrogen source reduces in water body when carrying out growth metabolism and bringing into play aerobic denitrification, thereby reach the object of the unwanted component such as nitrogen and sexavalent chrome in synchronous removal polluted-water.
It is electron acceptor(EA) that the chela billiard ball bacterium CGMCC5226 providing as above-mentioned can not only be take nitric nitrogen etc., and the mode of aerobic denitrification of take transforms nitrogen as nitrogen or nitrous oxide; And can take sexavalent chrome as electron acceptor(EA), by hexavalent chrome reduction, it is the trivalent chromium that toxicity is less, reach the object of removal, so this bacterial strain is not only applicable to water body or soil that nitrate and heavy metal pollute separately, and is applicable to water body or the soil of the two combined pollution.This bacterial strain is stronger to the adaptability of environment, can be 2-21 at C/N, and initial hexavalent chromium concentration is 0-40mg/L, and temperature is 20-52 ℃, at anoxic, anaerobism, micro-different dissolved oxygen concentration environment simultaneous denitrification such as aerobic and aerobic except sexavalent chrome.
One application of strain chela billiard ball bacterium in wastewater treatment, this bacterial strain is chela billiard ball bacterium (Chelatococcus daeguensis) TAD1, and deposit number is CGMCC NO.5226, and chela billiard ball bacterium CGMCC5226 is for synchronous nitrogen and the sexavalent chrome of removing waste water.
In described waste water, C/N is 2～21.
In described waste water, C/N is 12～18.
Carbon source in described waste water is one or more in sodium succinate, sodium acetate, Trisodium Citrate and waste molasses, and nitrogenous source is nitric nitrogen and/or ammonia nitrogen.
Concentration≤40mg/L of initial sexavalence Cr in described waste water.
In described waste water, the concentration of initial sexavalence Cr is 5～15mg/L.
The temperature of wastewater treatment is 30～50 ℃.
The temperature of wastewater treatment is 45～50 ℃.
PH is 7.0～9.0.
Described waste water is garbage leachate or electroplating wastewater.
Chela billiard ball bacterium of the present invention (Chelatococcus daeguensis) bacterial strain TAD1 has following beneficial effect in removing nitrogen and chromic application:
(1) this bacterial strain is aerobic denitrifying bacteria, has solved the difficult problem that traditional denitrification process nitrification and denitrification must separately carry out, and brief technical process, has reduced input cost.
(2) this bacterial strain can be realized simultaneous denitrification reduction of hexavalent chromium, envrionment conditions disunity and the problem to electron donor competition in denitrogenation dechromisation process have been solved well, the intermediate product that denitrification process produces simultaneously and reductase enzyme system can promote chromic reduction, have good economy, environment friendly.
(3) denitrification process, for producing alkali process, can not only make up the acidity that nitrifying process produces, and the system pH of making maintains neutral left and right, and can make the trivalent chromium producing be easy to precipitate and separate, therefore substantially without additional alkaline matter, has saved process costs.
(4) subject range of this bacterial strain is wider, not only can make microbial inoculum and strengthen existing denitrogenation except chromium process, and can combine for actual water body or soil remediation with other bacterial classifications or material, saves to greatest extent process costs.
Accompanying drawing explanation
In Fig. 1, a, b are respectively chela billiard ball bacterium TAD1 removal curve to nitrogen and Cr (VI) under different C/N conditions.
In Fig. 2, a, b are respectively chela billiard ball bacterium TAD1 removal curve to nitrogen and Cr (VI) under different Cr (VI) concentration.
The removal curve of Fig. 3 chela billiard ball bacterium TAD1 to COD, total nitrogen, nitric nitrogen and Cr (VI).
The removal curve of Fig. 4 chela billiard ball bacterium TAD1 to total chromium, Cr (VI).
Embodiment
Below in conjunction with accompanying drawing and specific embodiment, the present invention is further explained, but embodiments of the present invention are not limited to this.
Embodiment 1
Chela billiard ball bacterium (Chelatococcus daeguensis) TAD1 processes for waste landfill site leachate
By this bacterium, for the treatment of certain refuse landfill percolate, oozing drop liquid water quality testing data is COD3104mg/L, NO
3-N 5.45mg/L, NH
4-N 1129mg/L, pH 7.5～9, Cr (VI) 6.32mg/L, 45～50 ℃ of water temperatures.This bacterium is added in aeration and biological pond by 0.2% throwing bacterium amount (dry cell weight is 8g/L), fixing hydraulic detention time HRT=24h, the flow velocity of aerating system gas meter is 3L/min, after processing, water quality is COD 135.21mg/L, NO
3-N 0mg/L, NH
4-N 12.09mg/L, the corresponding pollutants removal rate of Cr (VI) 0mg/L is 95.64%, 100.00%, 98.92%, 100.00%.With the control group comparison that does not add this bacterium, COD, NO
3-N, NH
4the clearance of-N, Cr (VI) is respectively 61%, 55%, 79%, 20%.Therefore, this bacterium adds the obvious denitrification ability that improves system.
Embodiment 2
Chela billiard ball bacterium (Chelatococcus daeguensis) TAD1 processes for Industrial Wastewater Treatment
By this bacterium for the treatment of certain company's industrial chromium slag leach liquor, in leach liquor, containing hexavalent chromium concentration, be about 105.6mg/L, adjusting initial pH value is 7-7.5, this electroplating wastewater is introduced in biochemical treatment tank, added the TAD1 bacterium liquid of 10% enlarged culturing simultaneously, add 3% waste molasses, mixed solution is 45-50 ℃ of stir process, regularly detect hexavalent chromium concentration, after 24h, 48h, 72h, chromic reduction ratio reaches respectively 63.57%, 84.21%, 100%.
Embodiment 3
Nitrogenous source is the experiment except Cr (VI) impact on bacterial strain denitrogenation
Get laboratory glycerine pipe preserve t bacteria AD1 be seeded to 50mL sterilizing seed culture medium (every L substratum is containing 1g peptone, 0.5g yeast extract powder, 0.5g NaCl, pH=7), in temperature, be that 50 ℃, rotating speed are isothermal vibration activation culture 12h under 180r/min condition, it is that (every L substratum is containing 9.4g C for 5mg/L denitrification substratum containing Cr (VI) concentration that the bacterium liquid that then pipettes 10mL enlarged culturing is inoculated in 100mL sterilizing
4h
4na
2o
4, 1.0g KNO
3, 7.9g Na
2hPO
4, 1.5g KH
2pO
4, 0.1gMgSO
47H
2o, micro-1mL, pH=7), wherein Cr (VI) is with K
2cr
2o
7form add, controlled trial is set simultaneously, keep when other components unchanged of culture medium C/N, change nitrogenous source is Sodium Nitrite, ammonium chloride, cultivates under the same conditions 24h.Pipette 10 times of a certain amount of diluted samples, measure thalli growth optical density value OD
600; With the sample thief 15min of the centrifugal institute of 5000r/min, obtain supernatant liquor, measure Cr (VI) and nitrogen.
Experimental result is as shown in table 1.Take saltpetre as nitrogenous source, and thalli growth amount is maximum, reaches 0.347, is secondly ammonium nitrogen, and take Sodium Nitrite as nitrogenous source, and strain growth is the slowest, only has 0.074.After 24 hours, take nitric nitrogen, ammonium nitrogen, nitrous carbon nitrogen is nitrogenous source, and bacterial strain TAD1 reaches respectively 100%, 100%, 38.49% to Cr (VI) clearance; After 24 hours, nitric nitrogen, ammonium nitrogen, nitrite nitrogen decreasing ratio are respectively 98.43%, 19.04%, 84.92%.Illustrate that bacterial strain TAD1 can take nitric nitrogen, ammonium nitrogen and realize well denitrogenation except Cr (VI) as nitrogenous source.
Table 1 nitrogenous source is the impact except Cr (VI) on bacterial strain denitrogenation
Embodiment 4
C/N is the experiment except Cr (VI) impact on bacterial strain denitrogenation
(every L substratum is containing 9.4gC to configure the denitrification substratum that initial Cr (VI) content is 15mg/L
4h
4na
2o
4, 1.0g KNO
3, 7.9g Na
2hPO
4, 1.5g KH
2pO
4, 0.1g MgSO
47H
2o, micro-1mL, pH=7), keep the amount of nitrogenous source constant, adjusting C/N is 2,4,6,9,12,15,18 7 gradients, thereby changes C
4h
4na
2o
4add-on, wherein Cr (VI) is with K
2cr
2o
7form add, after sterilizing, pack 250mL Erlenmeyer flask into, then substratum accesses 10% enlarged culturing bacterium liquid, under 50 ℃, 150rpm condition, isothermal vibration is cultivated 24h, sampling and measuring nitrate nitrogen, nitrite nitrogen, Cr (VI) concentration and OD
600.
As seen from Figure 1, when C/N is 2,4,6,9,12, the NO of 139mg/L
3 --N clearance is respectively 13.68%, 18.86%, 30.23%, 66.83%, 91.04%, and C/N is 15,18 o'clock NO
3 --N clearance is greater than 95%, and all without nitrite nitrogen, accumulates in process; C/N was 2,4,6,9,12,15,18 o'clock originally, in initial 12h, Cr (VI) average removal rate is respectively 0.51,0.63,0.69,0.80,0.88,1.00,1.06mg/ (Lh), and after C/N surpasses 12, Cr (VI) realized all removals in 24 hours.Illustrate that bacterial strain TAD1 all can be to NO at low-carbon (LC) and rich carbocyclic ring border
3 --N and Cr (VI) bring into play certain removal and make embodiment 5
The experiment except Cr (VI) impact on bacterial strain denitrogenation of initial Cr (VI) concentration
(every L substratum is containing 11.75g C for configuration denitrification substratum
4h
4na
2o
4, 1.0g KNO
3, 7.9gNa
2hPO
4, 1.5g KH
2pO
4, 0.1g MgSO
47H
2o, micro-1mL, pH=7), to sterilizing denitrification substratum, add the different K that measure
2cr
2o
7stock solution, adjusts the initial Cr of substratum (VI) content and is 0,5,10,15,20,30, seven gradients of 40mg/L.Substratum accesses 10% enlarged culturing bacterium liquid, and under 50 ℃, 150rpm condition, isothermal vibration is cultivated 24h, every 4h, and sampling and measuring nitrate nitrogen, nitrite nitrogen, Cr (VI) concentration and OD
600.
As seen from Figure 2, initial Cr (VI) concentration is 0,5,10,15,20,30, during 40mg/L, in initial 12 hours, and NO
3 --N degradation rate is respectively 10.35,10.44,7.64,6.76,5.16,4.34,3.48mg/ (Lh), and Cr (VI) rate of reduction is respectively 0.42,0.73,1.01,0.95,0.79,0.78mg/ (Lh).After 24h, NO
3 --N clearance is respectively 98.52%, 99.19%, 98.32%, 94.52%, 76.31%, 56.39%, 37.35%, and Cr (VI) reduction ratio is respectively 100%, 100%, 100%, 100%, 84.81%, 57.27%, 42.01%.Illustrate that bacterial strain TAD1 more preponderates for the treatment of low levels Cr (VI) polluted-water, to high-content Cr (VI) polluted-water, also can bring into play removal effect, but need the cost longer time.
Embodiment 6
Temperature is the experiment except Cr (VI) impact on bacterial strain denitrogenation
(every L substratum is containing 11.75gC for the sterilizing denitrification substratum that is 15mg/L by initial Cr (VI) concentration
4h
4na
2o
4, 1.0g KNO
3, 7.9g Na
2hPO
4, 1.5g KH
2pO
4, 0.1g MgSO
47H
2o, micro-1mL, pH=7), access 10% enlarged culturing bacterium liquid, adjusting culture temperature is 30,35,40,45,50 ℃ of five gradients, and under 150rpm condition, isothermal vibration is cultivated 24h, measures nitrate nitrogen, nitrite nitrogen, Cr (VI) concentration and OD
600.
The impact of table 2 differing temps on TAD1 denitrification effect
Table 3 differing temps is the impact except Cr (VI) effect on TAD1
By table 2, table 3, learnt, when temperature is 30,35,40,45,50 ℃, after 24 hours, denitrification percent is respectively 63.68%, 74.20%, 87.23%, 90.18,94.53, Cr (VI) can all be removed, and in initial 12h, Cr (VI) rate of reduction is respectively 0.71,0.87,0.95,1.03,1.00mg/ (Lh).Illustrate that bacterial strain TAD1 has broad spectrum to the adaptation of temperature, be applicable to very much industrial application, than chemical process, as absorption waits, have more application potential.
Embodiment 7
In culturing process, COD, nitrogen, chromium change
(every L substratum is containing 11.75gC to configure the denitrification substratum that initial Cr (VI) concentration is 15mg/L
4h
4na
2o
4, 1.0g KNO
3, 7.9g Na
2hPO
4, 1.5g KH
2pO
4, 0.1g MgSO
47H
2o, micro-1mL, pH=7), access 10% enlarged culturing bacterium liquid, is that under 50 ℃, 180rpm condition, isothermal vibration is cultivated 24h in temperature, every 4h, sampling and measuring COD, total nitrogen, total chromium.
By Fig. 3, Fig. 4, can be found out, TAD1 is in whole denitrogenation except in Cr (VI) process, and COD degradation rate is 86.99%, and total nitrogen decreasing ratio is 37.50%, and total chromium decreasing ratio is 14.28%.Illustrate that bacterial strain TAD1 can realize the synchronous removal of carbon element, nitrogen, Cr (VI), and part nitre nitrogen reducible be N
2or N
2o; Identical with most of Cr (VI) reducing bacteria, bacterial strain TAD1 is mainly reduced to Cr (III) by the larger Cr (VI) of toxicity, rather than simply Cr (VI) is carried out to thing phase transition, extremely has market application foreground.
Claims (10)
1. the application of strain chela billiard ball bacterium in wastewater treatment, it is characterized in that, this bacterial strain is chela billiard ball bacterium (Chelatococcus daeguensis) TAD1, and deposit number is CGMCC NO.5226, and chela billiard ball bacterium CGMCC5226 is for synchronous nitrogen and the sexavalent chrome of removing waste water.
2. application according to claim 1, is characterized in that, in described waste water, C/N is 2～21.
3. application according to claim 2, is characterized in that, in described waste water, C/N is 12～18.
4. according to the application described in claim 1 or 2 or 3, it is characterized in that, the carbon source in described waste water is one or more in sodium succinate, sodium acetate, Trisodium Citrate and waste molasses, and nitrogenous source is nitric nitrogen and/or ammonia nitrogen.
5. according to the application described in claim 1 or 2 or 3, it is characterized in that concentration≤40mg/L of initial sexavalence Cr in described waste water.
6. application according to claim 5, is characterized in that, in described waste water, the concentration of initial sexavalence Cr is 5～15mg/L.
7. according to the application described in claim 1 or 2 or 3, it is characterized in that, the temperature of wastewater treatment is 30～50 ℃.
8. application according to claim 7, is characterized in that, the temperature of wastewater treatment is 45～50 ℃.
9. application according to claim 4, is characterized in that, in described waste water, the concentration of initial sexavalence Cr is 5～15mg/L, and the temperature of wastewater treatment is 45～50 ℃, and pH is 7.0～9.0.
10. according to the application described in claim 1 or 2 or 3, it is characterized in that, described waste water is garbage leachate or electroplating wastewater.
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| CN104560801A (en) * | 2014-12-25 | 2015-04-29 | 厦门大学 | Mesonia sp. as well as screening method and application thereof |
| CN110685266A (en) * | 2019-09-12 | 2020-01-14 | 武汉科技大学 | Method for improving mechanical property of calcareous sand by solidifying fiber reinforced microorganisms |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373169A (en) * | 2011-10-27 | 2012-03-14 | 华南理工大学 | Chelatococcus daeguensis with good aerobic denitrification performance and application thereof |
| CN103865821A (en) * | 2012-12-10 | 2014-06-18 | 中国石油天然气股份有限公司 | Chelatococcus sp., and preparation method and application thereof |
| KR20140093347A (en) * | 2013-01-15 | 2014-07-28 | 삼성전자주식회사 | Genes inducing agonistic effects by anti-c-Met antibody treatment and drug method using thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102373169A (en) * | 2011-10-27 | 2012-03-14 | 华南理工大学 | Chelatococcus daeguensis with good aerobic denitrification performance and application thereof |
| CN103865821A (en) * | 2012-12-10 | 2014-06-18 | 中国石油天然气股份有限公司 | Chelatococcus sp., and preparation method and application thereof |
| KR20140093347A (en) * | 2013-01-15 | 2014-07-28 | 삼성전자주식회사 | Genes inducing agonistic effects by anti-c-Met antibody treatment and drug method using thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560801A (en) * | 2014-12-25 | 2015-04-29 | 厦门大学 | Mesonia sp. as well as screening method and application thereof |
| CN110685266A (en) * | 2019-09-12 | 2020-01-14 | 武汉科技大学 | Method for improving mechanical property of calcareous sand by solidifying fiber reinforced microorganisms |
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