CN104153185A - Chlorine peroxidase catalytic oxidation hair decolorizing agent and decolorizing method and application - Google Patents
Chlorine peroxidase catalytic oxidation hair decolorizing agent and decolorizing method and application Download PDFInfo
- Publication number
- CN104153185A CN104153185A CN201410377930.9A CN201410377930A CN104153185A CN 104153185 A CN104153185 A CN 104153185A CN 201410377930 A CN201410377930 A CN 201410377930A CN 104153185 A CN104153185 A CN 104153185A
- Authority
- CN
- China
- Prior art keywords
- chloroperoxidase
- catalytic oxidation
- hair
- decolouring
- mixed solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Cosmetics (AREA)
Abstract
The invention discloses a chlorine peroxidase catalytic oxidation hair decolorizing agent and a decolorizing method and application. According to the hair decolorizing agent, based on the biological enzyme catalytic oxidation system, a phosphate buffer solution containing chloride ions is added into to-be decolorized black hair samples; the pH value of the solution is adjusted to 2.75-5, the mixture is soaked for 1 h and chlorine peroxidase is added into the mixture; then H2O2 is adopted as an oxidant and a H2O2 solution with the concentration of 30% is added into the mixed liquid; the mixture is mixed uniformly and the black hair is subjected to catalytic oxidation at the indoor temperature. According to the invention, the composition of the system is simple, the operation is convenient and the environmental protection is achieved, the dosage of the chlorine peroxidase and H2O2 is less, the reaction conditions are mild, the melanin in hair can be damaged, the decolorizing effect is obvious and the black hair samples are changed into yellowish brown in 24 hours.
Description
Technical field
The invention belongs to decolouring processing technology field, be specifically related to method and the application of a kind of chloroperoxidase catalytic oxidation hair decolorizer and decolouring.
Background technology
Along with scientific and technical progress and growth in the living standard, the idea of impelling people also changes occurring, and more people starts to pay close attention to the image of self.People are also more and more higher to beautiful pursuit, are no longer satisfied with single Black Human Hair, wish that the color of change hair changes the image of oneself.
Hair is a kind of natural protein fibre, and the natural colour in hair self forms in growth course, and these colors are mainly determined by melanin in human body.In general, melanin content is higher, and hair color is darker.Some strong oxidizers, can destroy melanin and make its decolouring, and the bleaching agent conventionally using in bleaching is hydrogen peroxide.Traditional method for bleaching can not obtain gratifying effect, again fiber is had to very large damage.Along with progressively improving and the continuous progress of the science of improving looks of health of people consciousness, safe, nontoxic, having no side effect to have concurrently meets consumer properties of product are constantly pursued, " green " bleaching agent becomes the development trend in following bleaching agent market day by day.Therefore, ripe bleaching process should accomplish selectively to remove the pigment in human hair, also wants the least possible damage keratin, to keep the mechanical property of human hair simultaneously.In addition, it is economical that bleaching agent is wanted, and technique, bleaching equipment want simple, and effect is reliable and stable, has good reappearance.
A kind of ferroheme peroxidase (42kDa) of separating in chloroperoxidase (CPO).CPO has oxidation activity very widely, have the catalytic characteristics of the plurality of enzymes such as ferroheme peroxidase (single electron and two electronics peroxidating, organic halogenation), catalase (hydrogen peroxide disproportionation, monohydric alcohol oxidation) and cytochrome P-450 (dealkylation, ethylene linkage epoxidation, sulfoxidation etc.) concurrently, be considered at present most widely used enzyme in peroxiredoxins, but take peroxidase, have not been reported as catalyst oxidation black hair decolouring.
Summary of the invention
The object of the invention is to overcome prior art defect, provide that a kind of safety, treatment process are simple, the chloroperoxidase catalytic oxidation hair decolorizer of good decolorizing effect and method and the application of decolouring.
The technical scheme of hair decolorizer of the present invention is:
Comprise chloroperoxidase, H
2o
2with the mixed solution that chlorion is configured to, wherein the chloroperoxidase concentration in mixed solution is 0.8 * 10
-2～1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2, chlorion mol ratio be (2.4 * 10
-5～3.75 * 10
-5): (2.90～3.95): (2.50～5.20).
The technical scheme of the method for hair decolouring of the present invention is:
Employing chloroperoxidase is catalyst, H
2o
2for oxidant, and chlorion is configured to mixed solution, and hair to be decoloured is immersed in mixed solution and is decoloured, and wherein the chloroperoxidase concentration in mixed solution is 0.8 * 10
-2～1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2, chlorion mol ratio be (2.4 * 10
-5～3.75 * 10
-5): (2.90～3.95): (2.50～5.20).
Described chlorion is the water-soluble generation of sodium chloride.
Comprise following concrete steps:
1) get sodium chloride and be dissolved in phosphate buffer formation mixed solution A, regulate pH value to 2.75～5 of mixed solution A, then hair to be faded is immersed in to 0.5～1h in mixed solution A;
2) count in molar ratio, then add chloroperoxidase and H
2o
2, mix and form mixed solution B, the decolouring of catalytic oxidation hair.
Described step 1) in, use hydrochloric acid to regulate pH value to 2.75～5 of mixed solution A.
Described phosphate buffer solution is for being used the KH of 0.1mol/L
2pO
4the K of solution and 0.1mol/L
2hPO
4solution allocation forms.
Described step 2) in, add 0.25mmolL
-1the H of chloroperoxidase solution and mass fraction 30%
2o
2the aqueous solution.
Described step 2) in, bleaching time is 12～24 hours.
Described step 1) and step 2) all carry out at ambient temperature.
The application of chloroperoxidase in the decolouring of catalytic oxidation hair.
The present invention relative to existing technologies, has following useful technique effect:
Hair decolorizer of the present invention is based on biological enzyme oxidation system, take chloroperoxidase as catalyst, H
2o
2for oxidant, can destroy melanin to reach the object of decolouring, but be different from general peroxidase, wherein substrate Cl
-after chloroperoxidase/hydrogen peroxide treatment, produce the little molecule of highly active chemistry as oxidation intermediates, can enter hair gap, make oxidation of melanin decolouring.Composing system of the present invention is simple, and the materials safety using is nontoxic, has no side effect, and operation is convenient, and environmental protection, can destroy the melanin in hair, and decolorizing effect is obvious.
In the method for hair decolouring of the present invention, hair is immersed to hair decolorizer can be completed, simple to operate, chloroperoxidase and H in the present invention
2o
2consumption is few, and cost is low, and reaction condition is gentle, and through evidence, in the time of 24 hours, black hair sample becomes yellowish-brown.System of the present invention has been used green biological oxidant, provides that a kind for the treatment of process is simple, cost is low, treatment effeciency is high, effective, eco-friendly chloroperoxidase catalysis H
2o
2the method of oxidised hair decolouring.
The present invention can be widely used in decolouring, especially the black hairs such as human hair, wig, animal hair.
Accompanying drawing explanation
Fig. 1 is the dominant mechanism figure of chloroperoxidase catalytic oxidation of the present invention.
The specific embodiment
Hair decolorizer of the present invention comprises: chloroperoxidase, H
2o
2with the mixed solution that chlorion is configured to, wherein the chloroperoxidase concentration in mixed solution is 0.8 * 10
-2～1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2, chlorion mol ratio be (2.4 * 10
-5～3.75 * 10
-5): (2.90～3.95): (2.50～5.20).
The concrete steps of hair discoloration method of the present invention are as follows:
At room temperature, get sodium chloride and be dissolved in phosphate buffer solution and form mixed solution A, use hydrochloric acid to regulate pH value to 2.75～5 that form mixed solution A, soak hair sample to be decoloured 0.5～1 hour, make in raw material immersion hair, after add 0.25 mmolL
-1chloroperoxidase, then to the H that adds mass fraction 30% in mixed liquor
2o
2solution, with H
2o
2for oxidant, mix and form mixed solution B, room temperature catalytic oxidation black hair decolouring 12～24 hours, the chloroperoxidase concentration in the preferred mixed solution of the present invention is 0.8 * 10
-2～1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be (2.4 * 10
-5～3.75 * 10
-5): (2.90～3.95): (2.50～5.20).
Wherein phosphate buffer solution is: use KH
2pO
4accurately prepare the KH of 0.1mol/L with distilled water
2pO
4solution; Use K
2hPO
43H
2o and distilled water are accurately prepared the K of 0.1mol/L
2hPO
4solution, then be mixedly configured into the solution of pH=5.8.
The dominant mechanism of chloroperoxidase catalytic oxidation of the present invention is as shown in Figure 1: be different from general peroxidase, wherein substrate Cl
-after chloroperoxidase/hydrogen peroxide treatment, produce highly active oxidation intermediates (chemical little molecule), can enter hair gap, make oxidation of melanin decolouring.Preferably at ambient temperature static 24 hours.Composing system of the present invention is simple, and operation is convenient, environmental protection, chloroperoxidase and H
2o
2consumption is few, and reaction condition is gentle, and cost is low, can effectively destroy the melanin in hair, and efficiency is high.
Below in conjunction with embodiment, the present invention is described in more detail, but protection scope of the present invention is not limited only to these embodiment.
Embodiment 1
20mg black hair sample to be decoloured is immersed in the phosphate buffer solution that contains sodium chloride completely, and the pH value to 2.75 of regulator solution, soaks one hour, after to add 150 μ L amount of substance concentration be 0.25mmolL
-1chloroperoxidase, and then be 30% H to adding mass fraction in mixed liquor
2o
2the aqueous solution, wherein the chloroperoxidase concentration in mixed solution is 1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be 3.75 * 10
-5: 3.95:5.2, mix rear room temperature catalytic oxidation black hair decolouring 24 hours, the color of observing black hair sample is yellowish-brown, decolorizing effect is obvious.
Embodiment 2
20mg black hair sample to be decoloured is immersed in the phosphate buffer solution that contains sodium chloride completely, and the pH value to 3 of regulator solution, soaks 0.5 hour, after to add amount of substance concentration be 0.25mmolL
-1chloroperoxidase, and then be 30% H to adding mass fraction in mixed liquor
2o
2the aqueous solution, wherein the chloroperoxidase concentration in mixed solution is 0.8 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be 2.4 * 10
-5: 3:2.5, mix rear room temperature catalytic oxidation black hair decolouring 15 hours, the color of observing black hair sample is yellowish-brown, decolorizing effect is obvious.
Embodiment 3
20mg black animal hair sample to be decoloured is immersed in the phosphate buffer solution that contains sodium chloride completely, and the pH value to 4 of regulator solution, soaks 0.7 hour, after to add amount of substance concentration be 0.25mmolL
-1chloroperoxidase, and then be 30% H to adding mass fraction in mixed liquor
2o
2the aqueous solution, wherein the chloroperoxidase concentration in mixed solution is 1.0 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be 3.0 * 10
-5: 2.9:4, mix rear room temperature catalytic oxidation black animal hair decolouring 16 hours, the color of observing black animal hair sample is yellowish-brown, decolorizing effect is obvious.
Embodiment 4
20mg black animal hair sample to be decoloured is immersed in the phosphate buffer solution that contains sodium chloride completely, and the pH value to 5 of regulator solution, soaks 0.6 hour, after to add amount of substance concentration be 0.25mmolL
-1chloroperoxidase, and then be 30% H to adding mass fraction in mixed liquor
2o
2the aqueous solution, wherein the chloroperoxidase concentration in mixed solution is 0.9 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be 2.7 * 10
-5: 3.2:4.5, mix rear room temperature catalytic oxidation black animal hair decolouring 12 hours, the color of observing black animal hair sample is yellowish-brown, decolorizing effect is obvious.
Embodiment 5
20mg black hair sample to be decoloured is immersed in the phosphate buffer solution that contains sodium chloride completely, and the pH value to 4.5 of regulator solution, soaks 0.8 hour, after to add amount of substance concentration be 0.25mmolL
-1chloroperoxidase, and then be 30% H to adding mass fraction in mixed liquor
2o
2the aqueous solution, wherein the chloroperoxidase concentration in mixed solution is 1.1 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be 3.3 * 10
-5: 3.5:3, mix rear room temperature catalytic oxidation black hair decolouring 18 hours, the color of observing black hair sample is yellowish-brown, decolorizing effect is obvious.
Embodiment 6
20mg black hair sample to be decoloured is immersed in the phosphate buffer solution that contains sodium chloride completely, and the pH value to 3.5 of regulator solution, soaks 0.9 hour, after to add amount of substance concentration be 0.25mmolL
-1chloroperoxidase, and then be 30% H to adding mass fraction in mixed liquor
2o
2the aqueous solution, wherein the chloroperoxidase concentration in mixed solution is 1.2 * 10
-2mmol/L, and chloroperoxidase, H
2o
2with the mol ratio of sodium chloride be 3.6 * 10
-5: 3.8:3.5, mix rear room temperature catalytic oxidation black hair decolouring 20 hours, the color of observing black hair sample is yellowish-brown, decolorizing effect is obvious.
Contrast test 1
In this contrast test, with the not phosphate buffer solution adjusting of chloride ion-containing, other steps are identical with embodiment 1, observe the color of black hair sample without obvious decolouring.
Contrast test 2
In this contrast test, do not add chloroperoxidase, other steps are identical with embodiment 1, and the color of observing black hair sample is without obviously decolouring.
By above contrast test, illustrated, chlorion and chloroperoxidase synergy produce decolorizing effect to hair.
Claims (10)
1. a chloroperoxidase catalytic oxidation hair decolorizer, is characterized in that: comprise chloroperoxidase, H
2o
2with the mixed solution that chlorion is configured to, wherein the chloroperoxidase concentration in mixed solution is 0.8 * 10
-2～1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2, chlorion mol ratio be (2.4 * 10
-5～3.75 * 10
-5): (2.90～3.95): (2.50～5.20).
2. a method for chloroperoxidase catalytic oxidation hair decolouring, is characterized in that: employing chloroperoxidase is catalyst, H
2o
2for oxidant, and chlorion is configured to mixed solution, and hair to be decoloured is immersed in mixed solution and is decoloured, and wherein the chloroperoxidase concentration in mixed solution is 0.8 * 10
-2～1.25 * 10
-2mmol/L, and chloroperoxidase, H
2o
2, chlorion mol ratio be (2.4 * 10
-5～3.75 * 10
-5): (2.90～3.95): (2.50～5.20).
3. the method for chloroperoxidase catalytic oxidation hair according to claim 2 decolouring, is characterized in that: described chlorion is the water-soluble generation of sodium chloride.
4. the method that chloroperoxidase catalytic oxidation hair according to claim 3 decolours, is characterized in that: comprise following concrete steps:
1) get sodium chloride and be dissolved in phosphate buffer formation mixed solution A, regulate pH value to 2.75～5 of mixed solution A, then hair to be faded is immersed in to 0.5～1h in mixed solution A;
2) count in molar ratio, then add chloroperoxidase and H
2o
2, mix and form mixed solution B, the decolouring of catalytic oxidation hair.
5. the method for chloroperoxidase catalytic oxidation hair decolouring according to claim 4, is characterized in that: described step 1), use hydrochloric acid to regulate pH value to 2.75～5 of mixed solution A.
6. the method for chloroperoxidase catalytic oxidation hair decolouring according to claim 4, is characterized in that: described phosphate buffer solution is for being used the KH of 0.1mol/L
2pO
4the K of solution and 0.1mol/L
2hPO
4solution allocation forms.
7. the method for chloroperoxidase catalytic oxidation hair decolouring according to claim 4, is characterized in that: described step 2), add 0.25mmolL
-1the H of chloroperoxidase solution and mass fraction 30%
2o
2the aqueous solution.
8. the method for chloroperoxidase catalytic oxidation hair decolouring according to claim 4, is characterized in that: described step 2), bleaching time is 12～24 hours.
9. according to the method for the chloroperoxidase catalytic oxidation hair decolouring described in claim 4-8 any one, it is characterized in that: described step 1) and step 2) all carry out at ambient temperature.
10. the application of chloroperoxidase in the decolouring of catalytic oxidation hair.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410377930.9A CN104153185B (en) | 2014-08-01 | 2014-08-01 | Chloroperoxidase catalysis oxidation hair decolorising agent and the method decolourized and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410377930.9A CN104153185B (en) | 2014-08-01 | 2014-08-01 | Chloroperoxidase catalysis oxidation hair decolorising agent and the method decolourized and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104153185A true CN104153185A (en) | 2014-11-19 |
| CN104153185B CN104153185B (en) | 2017-08-25 |
Family
ID=51878801
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410377930.9A Expired - Fee Related CN104153185B (en) | 2014-08-01 | 2014-08-01 | Chloroperoxidase catalysis oxidation hair decolorising agent and the method decolourized and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104153185B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104402121A (en) * | 2014-11-20 | 2015-03-11 | 陕西师范大学 | Method for catalyzing and degrading triazophos through chloroperoxidase |
| CN115096682A (en) * | 2022-08-26 | 2022-09-23 | 北京肿瘤医院(北京大学肿瘤医院) | Method for depigmenting malignant melanoma |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1296812A (en) * | 1999-09-24 | 2001-05-30 | 莱雅公司 | Composition for oxidation dyeing keratoprotein fibre and method for dyeing using said composition |
| CN1301320A (en) * | 1998-05-20 | 2001-06-27 | 诺沃挪第克生物化学北美公司 | Method for enzymatic treatment of wool |
| US6277806B1 (en) * | 1998-12-11 | 2001-08-21 | Unilever Home & Personal Care Usa, Division Of Conopco, Inc. | Bleaching enzymes and detergent compositions comprising them |
| US20070231283A1 (en) * | 2005-09-02 | 2007-10-04 | Manuela Javet | Process for bleaching keratin fibers |
| CN101781012A (en) * | 2009-12-31 | 2010-07-21 | 陕西师范大学 | Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2 |
-
2014
- 2014-08-01 CN CN201410377930.9A patent/CN104153185B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1301320A (en) * | 1998-05-20 | 2001-06-27 | 诺沃挪第克生物化学北美公司 | Method for enzymatic treatment of wool |
| US6277806B1 (en) * | 1998-12-11 | 2001-08-21 | Unilever Home & Personal Care Usa, Division Of Conopco, Inc. | Bleaching enzymes and detergent compositions comprising them |
| CN1296812A (en) * | 1999-09-24 | 2001-05-30 | 莱雅公司 | Composition for oxidation dyeing keratoprotein fibre and method for dyeing using said composition |
| US20070231283A1 (en) * | 2005-09-02 | 2007-10-04 | Manuela Javet | Process for bleaching keratin fibers |
| CN101781012A (en) * | 2009-12-31 | 2010-07-21 | 陕西师范大学 | Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104402121A (en) * | 2014-11-20 | 2015-03-11 | 陕西师范大学 | Method for catalyzing and degrading triazophos through chloroperoxidase |
| CN104402121B (en) * | 2014-11-20 | 2016-06-15 | 陕西师范大学 | The method of chloroperoxidase catalyzed degradation triazophos |
| CN115096682A (en) * | 2022-08-26 | 2022-09-23 | 北京肿瘤医院(北京大学肿瘤医院) | Method for depigmenting malignant melanoma |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104153185B (en) | 2017-08-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2579752C (en) | Novel laccase enzymes and their uses | |
| DE59607836D1 (en) | METHOD FOR PRODUCING A CHLORDIOXIDE-CONTAINING DISINFECTANT SOLUTION FOR WATER TREATMENT | |
| CN103358374A (en) | Method for bleaching liriodendron tulipifera wood for furniture | |
| Vats et al. | Decolorization of complex dyes and textile effluent by extracellular enzymes of Cyathus bulleri cultivated on agro-residues/domestic wastes and proposed pathway of degradation of Kiton blue A and reactive orange 16 | |
| CN1267212A (en) | Use of ascorbic acid in permanent waving and hair coloring compositions | |
| CN104153185A (en) | Chlorine peroxidase catalytic oxidation hair decolorizing agent and decolorizing method and application | |
| CN105567668B (en) | A kind of fur immersion complex enzyme formulation and preparation method thereof | |
| JPH01319413A (en) | Method for dyeing hair by continuous treatment using metal ion-containing composition and 5, 6-dihydroxyindole-2-carboxylic acid-containing dye composition | |
| CN103998016B (en) | Employ the dyeing of the keratin fiber of indole analog | |
| CZ206899A3 (en) | Oxidation composition and its use for dyeing, permanent deformation (permanent wave) or decoloration of keratin fibers | |
| CN102695495A (en) | Coloring composition containing an aromatic compound and tyrosinase | |
| CN101461593A (en) | Combined enzymolysis and solid-liquid biphase bleaching method for bleaching fresh water pearl | |
| Linke et al. | Carotene-degrading activities from Bjerkandera adusta possess an application in detergent industries | |
| CN1321180C (en) | Method for raising stability for preserving catalase prepared by microbiological method | |
| DK2645987T3 (en) | New Catalases | |
| CN101273809A (en) | Pearl albefaction method mediated by keratinase and combined with redox | |
| JP2003002898A (en) | Method for producing undecomposed silk fibroin solution and skin care agent containing the same | |
| CN100360736C (en) | Fast decorizing and whitening method of color feather and down | |
| CN105728036A (en) | Bovine serum albumin-platinum/bismuth composite nano material mimic peroxidase | |
| JP4767271B2 (en) | Production method of transparent colloidal silver | |
| JP2008274489A (en) | Method for bleaching indigo-dyed fabric | |
| CN107574022A (en) | A kind of compound enzyme detergent of final finishing | |
| CN113841685B (en) | Skeleton anticorrosion bleaching agent and application thereof | |
| WO2002102845A1 (en) | Process for producing silk fibroin-origin functional polypeptide and utilization thereof | |
| CN107034526B (en) | Cotton stalk treatment process and cotton stalk bark fibre preparation process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170825 Termination date: 20190801 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |