CN104152513A - Method for controlling microbiological contamination in validamycin fermentation technique - Google Patents
Method for controlling microbiological contamination in validamycin fermentation technique Download PDFInfo
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- CN104152513A CN104152513A CN201410372186.3A CN201410372186A CN104152513A CN 104152513 A CN104152513 A CN 104152513A CN 201410372186 A CN201410372186 A CN 201410372186A CN 104152513 A CN104152513 A CN 104152513A
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- jingganmycin
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Abstract
The invention relates to a method for controlling microbiological contamination in a validamycin fermentation technique, belonging to the technical field of fermentation techniques. The method comprises the following step: adding sulfuric acid streptin in the validamycin fermentation process. The method is reasonable in design; and a proper amount of sulfuric acid streptin is added at appropriate time in the validamycin fermentation process, thereby effectively inhibiting the growth of the infectious microbes and having the effect of increasing titer on the premise of ensuring the final titer.
Description
Technical field
The invention belongs to zymotechnique technical field, be specifically related to control in jingganmycin zymotechnique the method for microbiological contamination.
Background technology
Vetstrep: belong to microbiotic agricultural chemicals, disturb the synthetic and messenger ribonucleic acid of bacterioprotein to be combined with 3OS ribosomal subunit, suppress the prolongation of peptide chain, Gram-negative bacteria and positive bacteria etc. are had to good restraining effect.Bacterium to crop and fungoid disease all have obvious prevention effect.
Jingganmycin: be the agricultural antibiotic that has systemic action, when the mycelia of Rhizoctonia solani Kuhn touches after jingganmycin, can absorbed by somatic cells very soon and conduct in thalline, disturb and suppress somatic cells normal growth and grow, thereby playing therapeutic action.The scope of application is mainly used in rice sheath blight disease, also can be used for the control of the crop pests such as rice green smut, corn northern and southern leaf blight and vegetables and cotton, beans.Large-scale field is used result fully to show the feature of the environmental protection type agricultural chemical of its " preventive effect high, without poisoning, pollution-free " for many years, welcome by users at home and abroad.
To the control of rice sheath blight disease, in production, mainly rely on for many years jingganmycin and prevent and treat.Jingganmycin is that the mid-1970s research in last century finds to have drug effect high, has no side effect, to features such as environmental influence are little.
Therefore, guarantee that the normal production of jingganmycin is most important.But in the production process of jingganmycin, can encounter unavoidably microbiological contamination.Microbiological contamination is hard defects for a jingganmycin manufacturing enterprise, and especially at plum rain season, the microbiological contamination rate of jingganmycin is up to 20%.The cycle of the production of jingganmycin needs 7 days, and from the sub-tank of inclined plane inoculating, seed tank culture moves into fermentor tank again.On fermentor tank, microbiological contamination is also frequent thing, if microbiological contamination on fermentor tank this means that this cycle of this batch of jingganmycin is by all that has been achieved is spoiled.This loses great concerning enterprise.So, how can better control microbiological contamination, this will be the cardinal task of producing smoothly jingganmycin.
Summary of the invention
The problem existing for prior art, the object of the invention is to design provides the technical scheme of controlling the method for microbiological contamination in jingganmycin zymotechnique.
In described jingganmycin zymotechnique, control the method for microbiological contamination, it is characterized in that adding in jingganmycin fermenting process sulfate chain Mycinomycin II.
In described jingganmycin zymotechnique, control the method for microbiological contamination, it is characterized in that sulfate chain Mycinomycin II adds be opportunity: before disappearing, disappear after or shaking table cultivation stage.
In described jingganmycin zymotechnique, control the method for microbiological contamination, it is characterized in that sulfate chain Mycinomycin II add-on is: in every 100 grams of production feed liquids, add 0.02～0.04g sulfate chain Mycinomycin II.
Described sulfate chain Mycinomycin II is controlled the application of microbiological contamination in jingganmycin zymotechnique.
In above-mentioned jingganmycin zymotechnique, control the method for microbiological contamination, reasonable in design, in jingganmycin fermenting process, under suitable opportunity, add the sulfate chain Mycinomycin II of appropriate amount, not only can effectively suppress the growth of miscellaneous bacteria, and in ensureing final tiring, have and increase the effect of tiring.
Embodiment
Further illustrate the present invention below in conjunction with embodiment.
Embodiment 1:
One, experimental technique
1) Vetstrep dosage: 72% agricultural Vetstrep (soluble powder) amount of formulation is 13.8～27.8 grams, is watered 50～75 kilograms;
2) Vetstrep add-on: in the shaking flask of 50g feed liquid, need add the agricultural Vetstrep of 0.0138g;
3) fermention medium is (%): starch 12%, yeast powder 4%, KH
2pO
40.05%, NaCl 0.15%, water surplus; The most adaptable method is: fermentation Initial pH 7.2, inoculum size 8%, 37 DEG C of leavening temperatures;
4) in test, agricultural Vetstrep adds by three kinds of modes: before A disappears, add shaking flask; After disappearing, B adds shaking flask; C shaking table is cultivated 50h and is added shaking flask.Three kinds of modes, screening best mode.
Two, experimental data
Upper shaking flask time 2013.10.8-10.15 shaking table time 150h
Three, experimental result
Before A disappears, add shaking flask, put cylinder total hundred million and improve 0.83%; After B disappears, add shaking flask, put cylinder total hundred million and improve 0.96%; C shaking table is cultivated 50h and is added shaking flask, puts cylinder total hundred million and improves 1.16%.As can be seen here: add the best moment of agricultural streptomycin to be, shaking table is cultivated 50h and added shaking flask.
Embodiment 2:
One, experimental technique
1) Vetstrep dosage: 72% agricultural Vetstrep (soluble powder) amount of formulation is 13.8～27.8 grams, is watered 50～75 kilograms;
2) Vetstrep add-on: in the fermentor tank of 50 tons of feed liquids, need add the agricultural Vetstrep of 13.8kg;
3) fermention medium is (%): starch 12%, yeast powder 4%, KH
2pO
40.05%, NaCl 0.15%, water surplus; The most adaptable method is: fermentation Initial pH 7.2, inoculum size 8%, 37 DEG C of leavening temperatures;
4) in test, agricultural Vetstrep adds by three kinds of modes: before A disappears, add shaking flask; After disappearing, B adds shaking flask; C shaking table is cultivated 50h and is added shaking flask.Wherein A adds shaking flask before disappearing, and puts cylinder total hundred million and improves 0.83%; After B disappears, add shaking flask, put cylinder total hundred million and improve 0.96%; C shaking table is cultivated 50h and is added shaking flask, puts cylinder total hundred million and improves 1.16%.As can be seen here: add the best moment of agricultural streptomycin to be, shaking table is cultivated 50h and added shaking flask.
Two, experimental data
Three, experimental result
This list data explanation adds Vetstrep in fermentor tank, can improve tiring of jingganmycin.Therefore, can on fermentor tank, add Vetstrep tank to produce.
Embodiment tri-:
One, experimental technique
1) Vetstrep dosage: 72% agricultural Vetstrep (soluble powder) amount of formulation is 13.8～27.8 grams, is watered 50～75 kilograms;
2) Vetstrep add-on: in the fermentor tank of 50 tons of feed liquids, need add the agricultural Vetstrep of 13.8kg;
3) fermention medium is (%): starch 12%, yeast powder 4%, KH
2pO
40.05%, NaCl 0.15%, water surplus; The most adaptable method is: fermentation Initial pH 7.2, inoculum size 8%, 37 DEG C of leavening temperatures;
4) in test, agricultural Vetstrep adds by three kinds of modes: before A disappears, add shaking flask; After disappearing, B adds shaking flask; C shaking table is cultivated 50h and is added shaking flask.Wherein A adds shaking flask before disappearing, and puts cylinder total hundred million and improves 0.83%; After B disappears, add shaking flask, put cylinder total hundred million and improve 0.96%; C shaking table is cultivated 50h and is added shaking flask, puts cylinder total hundred million and improves 1.16%.As can be seen here: add the best moment of agricultural streptomycin to be, shaking table is cultivated 50h and added shaking flask.
Two, experimental data
Three, experimental result
12.1-12.12 for fermentor tank is with Vetstrep testing data, and 12.16-12.27 for fermentor tank is not with Vetstrep testing data.Wherein in form, red data is microbiological contamination tank data, the normal tank data of black positive.12.1-12.12 microbiological contamination rates are that 16.67%, 12.16-12.27 microbiological contamination rates are 28.33%.On average improve 2.67%.Testing data shows, agricultural Vetstrep can be suppressed other bacterial growth not to be affected jingganmycin and tire, can improve on the contrary jingganmycin and tire, and be company's create beneficial result.
In addition, in the fermentor tank of 50 tons of feed liquids, need add the agricultural Vetstrep of 10kg, 15kg and 20kg, finally also can reach the technique effect identical with embodiment tri-.
Claims (4)
1. in jingganmycin zymotechnique, control the method for microbiological contamination, it is characterized in that adding in jingganmycin fermenting process sulfate chain Mycinomycin II.
2. in jingganmycin zymotechnique as claimed in claim 1, control the method for microbiological contamination, it is characterized in that sulfate chain Mycinomycin II adds be opportunity: before disappearing, disappear after or shaking table cultivation stage.
3. in jingganmycin zymotechnique as claimed in claim 1, control the method for microbiological contamination, it is characterized in that sulfate chain Mycinomycin II add-on is: in every 100 grams of production feed liquids, add 0.02～0.04g sulfate chain Mycinomycin II.
4. sulfate chain Mycinomycin II is controlled the application of microbiological contamination in jingganmycin zymotechnique.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080292560A1 (en) * | 2007-01-12 | 2008-11-27 | Dov Tamarkin | Silicone in glycol pharmaceutical and cosmetic compositions with accommodating agent |
| CN101851653A (en) * | 2010-05-17 | 2010-10-06 | 上海交通大学 | Fermentation production method of validamycin A |
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2014
- 2014-07-31 CN CN201410372186.3A patent/CN104152513A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080292560A1 (en) * | 2007-01-12 | 2008-11-27 | Dov Tamarkin | Silicone in glycol pharmaceutical and cosmetic compositions with accommodating agent |
| CN101851653A (en) * | 2010-05-17 | 2010-10-06 | 上海交通大学 | Fermentation production method of validamycin A |
Non-Patent Citations (7)
| Title |
|---|
| 中国兽药典委员会: "《中华人民共和国兽药典 兽药使用指南 化学药品卷 2010年版》", 30 April 2011 * |
| 周启等: "《全国高等农业院校教材 农用抗生素和微生物杀虫剂 微生物专业用书》", 31 May 1995 * |
| 姜巨全等: "《微生物生理学》", 30 April 2014 * |
| 师海波等: "《最新临床药物手册 第3版》", 31 January 2013 * |
| 戴水莲等: "PDA培养基中加入青霉素、链霉素的抗菌作用试验简报", 《中国食用菌》 * |
| 熊智强等: "红谷霉素生料发酵工艺的研究", 《中国生物工程杂志》 * |
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Application publication date: 20141119 |
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