CN104151592B - Method for improving mechanical properties of silk protein membrane by virtue of biological mineralization - Google Patents
Method for improving mechanical properties of silk protein membrane by virtue of biological mineralization Download PDFInfo
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- CN104151592B CN104151592B CN201410372692.2A CN201410372692A CN104151592B CN 104151592 B CN104151592 B CN 104151592B CN 201410372692 A CN201410372692 A CN 201410372692A CN 104151592 B CN104151592 B CN 104151592B
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims abstract description 8
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- 239000003960 organic solvent Substances 0.000 claims abstract description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000019253 formic acid Nutrition 0.000 claims abstract description 4
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- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 50
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 50
- 239000008367 deionised water Substances 0.000 claims description 16
- 229910021641 deionized water Inorganic materials 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000012224 working solution Substances 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 11
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 108010013296 Sericins Proteins 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 241000255794 Bombyx mandarina Species 0.000 claims description 4
- 241000255789 Bombyx mori Species 0.000 claims description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- 229920001872 Spider silk Polymers 0.000 claims description 3
- 238000013459 approach Methods 0.000 claims description 3
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- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 claims description 2
- 210000004907 gland Anatomy 0.000 claims description 2
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Abstract
The invention discloses a method for improving mechanical properties of a silk protein membrane by virtue of biological mineralization. The method is realized by the following steps of dissolving an aqueous solution of silk protein or silk protein powder in organic solvents such as hexafluoroacetone, hexafluoroisopropanol and formic acid to obtain a silk protein solution, then dropwises adding the aqueous solution of silk protein or the silk protein solution to a plastic plate and drying to obtain the silk protein membrane. By adopting the method disclosed by the invention. The mechanical properties of the silk protein membrane are significantly improved and the time is greatly saved and the method has the advantages of short production period, simple and efficient extraction process and no complicated and tedious mineralization process and is simple to operate.
Description
Technical field
The present invention relates to a kind of mineralization method of silk protein membrane, especially relate to one kind and significantly improve silk-fibroin membrane forces
Learn the mineralization method of performance, the processing method belonging to material.
Background technology
Hydroxyapatite has good biocompatibility, is the main inorganic composition constituting skeleton and tooth.Entirely
The Cranial defect patient up to tens of millions of people that the world causes because of various factors such as wound, disease, heredity every year.Simulation natural bone master
Want composition, by mineral matter and material combine preparation biological support so that Bone Defect Repari become a kind of may.By natural polymer
The method by mineralising for the sub- material, can prepare the timbering material close on physico-chemical property with natural bone component, and this is also
It is conventional method in biologic bracket material preparation.
The wire protein fibers such as Juvenile Hormone, wild silkworm silk-fibroin (tussah silk and wild silk yarn etc.) and spider silk are natural height
Molecule.Wire protein fiber has good biocompatibility and mechanical strength, is a kind of degradable biological macromolecular material, its
Catabolite has no toxic side effect to cell or body, also seldom produces immunological rejection or inflammatory reaction, using silk-fibroin
Silk-fibroin can be processed into porous support or membrane material by these advantages, then carries out biomineralization, for scientific research with face
Bed application.But common mineralization methods (physics or chemical mineralising) make the mechanical property of silk protein membrane significantly reduce, and
And generally existing mineralising is not exclusively, easily generates the debris beyond hydroxyapatite, therefore can not meet scientific research and life
The needs producing.If publication date is on 07 04th, 2012, in the Chinese patent of Publication No. cn102532573a, disclose one kind
The quick molding method of sericin/hydroxyapatite composite membrane, the method is that sericin/hydroxyapatite composite membrane is entered
Row rapid shaping, but easily generate the accessory substance of hydroxyapatite.
In sum, there is presently no a kind of operation simple, processing ease, mineralizer is pure, can improve silk protein membrane again
The biomimetic mineralization method of mechanical property.
Content of the invention
It is an object of the invention to overcoming above-mentioned deficiency present in prior art, using biomimetic mineralization method, by silk
Some functional groups (as carboxyl) in protein peptide chain, to induce hydroxyapatite in the nucleation on silk protein membrane surface, rapidly make
Hydroxyapatite deposition is on silk protein membrane surface.In this mineralising system, hydroxyapatite provides strong rigidity, and silk egg
Tunica albuginea provides larger ductility so that hydroxyapatite is had complementary advantages with silk protein membrane, invents one kind and significantly improves
The new mineralization methods of silk protein membrane mechanical property.
The present invention solve the above problems be employed technical scheme comprise that as follows:
A kind of method improving silk protein membrane mechanical property by biomineralization, specifically includes following steps:
(1) silk protein aqueous solution or silk-fibroin powder are dissolved in the organic solvents such as Hexafluoro acetone, hexafluoroisopropanol, formic acid
In, obtain silk protein solution, then silk protein aqueous solution or silk protein solution are added drop-wise on plastic plate, after being dried, obtain an egg
Tunica albuginea；
(2) silk protein membrane in step (1) is post-processed, obtained insoluble silk protein membrane；
(3) silk protein membrane in step (2) be impregnated in the cacl for 6.0-8.0 for the ph value2Soak half an hour in the aqueous solution,
Obtain the tussah silk protein membrane of pre- mineralising；
(4) a certain amount of nanometer hydroxyapatite powder is added in 40ml deionized water, adding 0.64ml concentration is
0.1 mole every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) by the hydroxyapatite working solution in step (4), add 3gnacl and 200ml deionized water, continuously stirred equal
Even, obtain hydroxylapatite mineralized liquid, the silk protein membrane of the pre- mineralising in step (3) is put in hydroxylapatite mineralized liquid and stirs
Mix and slowly ph is adjusted to 7.4；
(6) continuously stirred 0.1-48h, temperature control in 4-100 degree so that silk protein membrane surface gradually deposited hydroxyl phosphorus ash
Stone crystal, obtains the silk protein membrane of mineralising；
(7) Mechanics Performance Testing shows that the silk protein membrane mechanical property of mineralising significantly improves, and infrared spectrum and x- ray spread out
Penetrate result and show that the mineralising deposit obtaining is single hydroxyapatite crystal.Cell experiment shows people's msc cell and mg-63
Cell can adhere to and propagation on the silk protein membrane of mineralising, has good biocompatibility.
As further improving, step (1) intermediate filment species of the present invention is bombyx mori silk fibroin, sericin, silk
Any one in gland albumen, wild silkworm fibroin, sericin, spider silk fibroin, recombinant fibroin, tussah silk-fibroin.
As further improving, in step (1) of the present invention, the organic solvent of dissolving silk-fibroin be Hexafluoro acetone,
Any one in hexafluoroisopropanol, acetic acid, formic acid.
As further improving, the post-processing approach in step (2) of the present invention, is to be immersed in silk protein membrane to contain
0.1-48h is processed in solution in one or more of methyl alcohol, ethanol, methyl ether, ether, acetone and methyl propanediol.
As further improving, in step (4) of the present invention, using the hydroxyapatite powder arriving be self-control or
Commercially available.
As further improving, in step (4) of the present invention, hydroxyapatite working solution is configured, the hydroxyl of addition
Base apatite powder and deionized water quality are than for 1:10-1:1000
As further improving, in step (5) of the present invention, silk protein membrane and mineralized liquid mass ratio are 1:10-1:
200.
Method process is simple of the present invention, processing ease, short preparation period, good biocompatibility.By this ore deposit
Change method, mineralising hydroxyapatite is attached to the surface of silk protein membrane it can be ensured that the inorganic mineralizer obtaining is hydroxy-apatite
Stone crystal, hydroxyapatite purity is very high.This method cannot be only used for silkworm, wild silkworm (tussah silk and wild silk yarn etc.) and spider
Deng the mineralising of silk protein membrane, or even the mineralising that can be used for other macromolecular materials.The mineralized fibroin membrane material of present invention preparation
There is biocompatibility.Tussah silk peptide film Young's modulus after the method mineralising, up to 70mpa, is common tussah silk peptide film
2.5 times.This invention enriches the type of biomineralization, is biomaterial and field of tissue engineering technology offer new approaches, has wide
Wealthy application prospect.
Due to the utilization of technique scheme, the present invention compared with prior art has a feature following outstanding:
(1) significantly improve the mechanical property (including elastic modelling quantity and maximum stress) of silk protein membrane, by system of the present invention
Compared with the biomembrane that the biomembrane that standby finished product is made is made with bombyx mori silk fibroin or other natural polymer albumen, have
Very high elastic modelling quantity and stress, thus reach scientific research and clinical medical application.
(2) simple to operate, with short production cycle: extraction process is simple, efficient, there is no complicated loaded down with trivial details mineralization process, time
On greatly save.
(3) because using high-purity hydroxyapatite crystal, the crystal therefore obtaining in mineralization process is hydroxyl phosphorus all the time
Lime stone, does not have the pollution of other debris.
(4) good biocompatibility: silk protein membrane or degradation product are to cell or tissue non-toxic reaction；Cell experiment
Show higher cell adherence rate and the rate of increase, there is good biocompatibility, biological safety is high, biological doctor can be met
Application in.
Brief description
Fig. 1-for the different silk protein membrane mechanical property of several mineralising times in embodiment 1 comparison, wherein, asf is toothed oak
Silkworm fibroin membrane；Asf-8h is the tussah silk peptide film of mineralising 8h；Asf-24h is the tussah silk peptide film of mineralising 24h.
Specific embodiment
Below by embodiment, the present invention is described in further detail, following examples be explanation of the invention and
The invention is not limited in following examples.
Embodiment 1
In the present embodiment, the method for biomineralization raising silk protein membrane mechanical property in turn includes the following steps:
(1) Tussah Silk Fibroin Powder is dissolved in Hexafluoro acetone machine solvent, obtains tussah silk fibroin solution, then by tussah silk
Fibroin solution is added drop-wise on plastic plate, obtains silk protein membrane after being dried；
(2) process 12h in the middle of the alcohol silk protein membrane in step (1) being immersed in 75%, obtain insoluble tussah
Fibroin protein film；
(3) tussah silk fibroin film in step (2) be impregnated in the cacl for 6.0 for the ph value2Half is soaked little in the aqueous solution
When, obtain the tussah silk fibroin film of pre- mineralising；
(4) 1g nanometer hydroxyapatite powder is added in 40ml deionized water, adds 0.64ml concentration and rub for 0.1
You every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) by the hydroxyapatite working solution in step (4), add 3gnacl and 200ml deionized water, continuously stirred equal
Even, obtain hydroxylapatite mineralized liquid, the tussah silk fibroin film of the pre- mineralising in step (3) is put into hydroxylapatite mineralized
In liquid, ph is simultaneously slowly adjusted to 7.4 by stirring；
(6) continuously stirred 8h, temperature control is at 25 degree so that tussah silk fibroin film surface gradually deposited hydroxyl apatite
Crystal, obtains the tussah silk fibroin film of mineralising；
(7) infrared spectrum and X-ray diffraction result show that the deposit on the mineralising tussah silk fibroin film surface obtaining is
Single hydroxyapatite crystal；
(8) tussah silk fibroin film of this mineralising of Mechanics Performance Testing, Young's modulus is up to 60mpa.
Elastic modelling quantity produces the index of elastic deformation complexity as weighing material, and its value is bigger, makes material occur one
The stress determining elastic deformation is also bigger, and that is, material stiffness is bigger.Fig. 1 is different silk-fibroin of several mineralising times in embodiment 1
The comparison of film elastic modelling quantity, asf is tussah silk peptide film；Asf-8h is the tussah silk peptide film of mineralising 8h；Asf-24h is mineralising 24h
Tussah silk peptide film.As a control group, in non-mineralising, its Young's modulus is about 33mpa to tussah silk peptide film；After mineralising 8 hours,
Young's modulus is about 50mpa；After mineralising 24 hours, Young's modulus is about 61mpa, and compared with tussah silk peptide film, difference is extremely aobvious
Write, * * p < 0.01.Illustrate to significantly improve the mechanical property of silk protein membrane by this mineralization methods, meanwhile, during with mineralising
Between increase, the mechanical property of silk-fibroin mineralising film is also gradually increased.
Embodiment 2
The mineralization method promoting the tussah silk peptide of cell growth in the present embodiment in turn includes the following steps:
(1) silk fibroin powder is dissolved in hexafluoroisopropanol, obtains silk fibroin protein solution, then silk protein solution is dripped
To on plastic plate, after being dried, obtain fibroin protein film；
(2) fibroin protein film in step (1) is immersed in process 24h in 75% alcohol, obtains insoluble fibroin
Protein film；
(3) fibroin protein film in step (2) be impregnated in the cacl for 7.0 for the ph value2Soak half an hour in the aqueous solution, obtain
Fibroin protein film to pre- mineralising；
(4) 2g nanometer hydroxyapatite powder is added in 40ml deionized water, adds 0.64ml concentration and rub for 0.1
You every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) the hydroxyapatite working solution in step (4) is added 3gnacl and 200ml deionized water, continuously stirred equal
Even, obtain hydroxylapatite mineralized liquid, the fibroin protein film of the pre- mineralising in step (3) is put in hydroxylapatite mineralized liquid
Ph is simultaneously slowly adjusted to 7.4 by stirring；
(6) continuously stirred 24h, temperature control is at 25 degree so that gradually deposited hydroxyl apatite is brilliant on fibroin protein film surface
Body, obtains the fibroin protein film of mineralising；
(7) infrared spectrum and X-ray diffraction result show that the deposit on the mineralising fibroin protein film surface obtaining is single
Hydroxyapatite crystal；
(8) tussah silk fibroin film of this mineralising of Mechanics Performance Testing, Young's modulus is up to 70mpa.
Embodiment 3
In the present embodiment, the method for biomineralization raising silk protein membrane mechanical property in turn includes the following steps:
(1) Tussah Silk Fibroin Powder is dissolved in Hexafluoro acetone machine solvent, obtains tussah silk fibroin solution, then by tussah silk
Fibroin solution is added drop-wise on plastic plate, obtains silk protein membrane after being dried；
(2) process 12h in the middle of the alcohol silk protein membrane in step (1) being immersed in 75%, obtain insoluble tussah
Fibroin protein film；
(3) tussah silk fibroin film in step (2) be impregnated in the cacl for 6.0 for the ph value2Half is soaked little in the aqueous solution
When, obtain the tussah silk fibroin film of pre- mineralising；
(4) 1g nanometer hydroxyapatite powder is added in 40ml deionized water, adds 0.64ml concentration and rub for 0.1
You every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) by the hydroxyapatite working solution in step (4), add 3gnacl and 200ml deionized water, continuously stirred equal
Even, obtain hydroxylapatite mineralized liquid, the tussah silk fibroin film of the pre- mineralising in step (3) is put into hydroxylapatite mineralized
In liquid, ph is simultaneously slowly adjusted to 7.4 by stirring；
(6) continuously stirred 24h, temperature control at 25 degree so that tussah silk fibroin film surface gradually deposited hydroxyl phosphorus ash
Stone crystal, obtains the tussah silk fibroin film of mineralising.
(7) mg-63 cell is seeded on the tussah silk fibroin film of this mineralising, and cell can be in the tussah silk peptide egg of mineralising
On tunica albuginea, adhesion and propagation, have good biocompatibility.
Embodiment 4
In the present embodiment, the method for biomineralization raising silk protein membrane mechanical property in turn includes the following steps:
(1) Tussah Silk Fibroin Powder is dissolved in Hexafluoro acetone machine solvent, obtains tussah silk fibroin solution, then by tussah silk
Fibroin solution is added drop-wise on plastic plate, obtains silk protein membrane after being dried；
(2) process 12h in the middle of the alcohol silk protein membrane in step (1) being immersed in 75%, obtain insoluble tussah
Fibroin protein film；
(3) tussah silk fibroin film in step (2) be impregnated in the cacl for 6.0 for the ph value2Half is soaked little in the aqueous solution
When, obtain the tussah silk fibroin film of pre- mineralising；
(4) 1g nanometer hydroxyapatite powder is added in 40ml deionized water, adds 0.64ml concentration and rub for 0.1
You every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) by the hydroxyapatite working solution in step (4), add 3gnacl and 200ml deionized water, continuously stirred equal
Even, obtain hydroxylapatite mineralized liquid, the tussah silk fibroin film of the pre- mineralising in step (3) is put into hydroxylapatite mineralized
In liquid, ph is simultaneously slowly adjusted to 7.4 by stirring；
(6) continuously stirred 24h, temperature control at 25 degree so that tussah silk fibroin film surface gradually deposited hydroxyl phosphorus ash
Stone crystal, obtains the tussah silk fibroin film of mineralising.
(7) people msc cell is seeded on the tussah silk fibroin film of this mineralising, and cell can be in the tussah silk peptide egg of mineralising
On tunica albuginea, adhesion and propagation, have good biocompatibility.
Embodiment 5
In the present embodiment, the method for biomineralization raising silk protein membrane mechanical property in turn includes the following steps:
(1) Tussah Silk Fibroin Powder is dissolved in Hexafluoro acetone machine solvent, obtains tussah silk fibroin solution, then by tussah silk
Fibroin solution is added drop-wise on plastic plate, obtains silk protein membrane after being dried；
(2) process 12h in the middle of the alcohol silk protein membrane in step (1) being immersed in 75%, obtain insoluble tussah
Fibroin protein film；
(3) tussah silk fibroin film in step (2) be impregnated in the cacl for 6.0 for the ph value2Half is soaked little in the aqueous solution
When, obtain the tussah silk fibroin film of pre- mineralising；
(4) 1g nanometer hydroxyapatite powder is added in 40ml deionized water, adds 0.64ml concentration and rub for 0.1
You every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) by the hydroxyapatite working solution in step (4), add 3gnacl and 200ml deionized water, continuously stirred equal
Even, obtain hydroxylapatite mineralized liquid, the tussah silk fibroin film of the pre- mineralising in step (3) is put into hydroxylapatite mineralized
In liquid, ph is simultaneously slowly adjusted to 7.4 by stirring；
(6) continuously stirred 8h, temperature control is at 25 degree so that tussah silk fibroin film surface gradually deposited hydroxyl apatite
Crystal, obtains the tussah silk fibroin film of mineralising.
(7) people hek-293 cell is seeded on the tussah silk fibroin film of this mineralising, and cell can be in the tussah silk of mineralising
On fibroin film, adhesion and propagation, have good biocompatibility.
Last in addition it is also necessary to it is noted that listed above be only the present invention be embodied as example.Obviously, the present invention is not
It is limited to above example, can also have many deformation.Those of ordinary skill in the art can be straight from present disclosure
Connect all deformation derived or associate, be all considered as protection scope of the present invention.
Claims (7)
1. a kind of by biomineralization improve silk protein membrane mechanical property method it is characterised in that: specifically comprise the following steps that
(1) silk protein aqueous solution or silk protein solution are added drop-wise on plastic plate, after being dried, obtain silk protein membrane；Described silk
Protein solution is the solution that the dissolving of silk-fibroin powder obtains in organic solvent；
(2) silk protein membrane in step (1) is post-processed, obtained insoluble silk protein membrane；
(3) silk protein membrane in step (2) be impregnated in the cacl for 6.0-8.0 for the ph value2Soak half an hour in the aqueous solution, obtain
The silk protein membrane of pre- mineralising；
(4) a certain amount of nanometer hydroxyapatite powder is added in 40ml deionized water, adds 0.64ml concentration and rub for 0.1
You every liter of hcl, ultrasonic 10 minutes so as to form homogeneous milky suspension, be hydroxyapatite working solution；
(5) by the hydroxyapatite working solution in step (4), 3g nacl and 200ml deionized water are added, continuously stirred uniform,
Obtain hydroxylapatite mineralized liquid, the silk protein membrane of the pre- mineralising in step (3) is put into stirring in hydroxylapatite mineralized liquid
And slowly ph is adjusted to 7.4；
(6) continuously stirred 0.1-48h, temperature control is in 4-100 degree so that gradually deposited hydroxyl apatite is brilliant on silk protein membrane surface
Body, obtains the silk protein membrane of mineralising.
2. according to claim 1 by biomineralization improve silk protein membrane mechanical property method it is characterised in that: institute
Stating step (1) intermediate filment species is bombyx mori silk fibroin, silk gland protein, wild silkworm fibroin, sericin, spider silk fibroin, restructuring silk
Any one in albumen, tussah silk-fibroin.
3. according to claim 1 by biomineralization improve silk protein membrane mechanical property method it is characterised in that: institute
State in step (1), described organic solvent is Hexafluoro acetone, hexafluoroisopropanol, acetic acid, any one in formic acid.
4. according to claim 1 by biomineralization improve silk protein membrane mechanical property method it is characterised in that: institute
State the post-processing approach in step (2), be that silk protein membrane is immersed in containing methyl alcohol, ethanol, methyl ether, ether, acetone and methyl-prop
0.1-48h is processed in solution in one or more of glycol.
5. according to claim 1 by biomineralization improve silk protein membrane mechanical property method it is characterised in that: institute
State in step (4), described hydroxyapatite powder is to make by oneself or commercially available.
6. according to claim 1 by biomineralization improve silk protein membrane mechanical property method it is characterised in that: institute
State in step (4), hydroxyapatite working solution configured, the hydroxyapatite powder of addition and deionized water quality ratio is for 1:
10-1:1000.
7. according to claim 1 by biomineralization improve silk protein membrane mechanical property method it is characterised in that: institute
State in step (5), silk protein membrane is 1:10-1:200 with the mass ratio of mineralized liquid.
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| CN1689653A (en) * | 2004-04-29 | 2005-11-02 | 中南大学 | Preparation method of biological composite material of hydroxyapatite and high polymer |
| CN102532573A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Rapid molding method of sericin/hydroxyapatite composite membrane |
| CN102850565A (en) * | 2012-08-10 | 2013-01-02 | 浙江大学 | Biological mineralization method of tussah silk fibroin for promoting cell growth |
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| CN102532573A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Rapid molding method of sericin/hydroxyapatite composite membrane |
| CN102850565A (en) * | 2012-08-10 | 2013-01-02 | 浙江大学 | Biological mineralization method of tussah silk fibroin for promoting cell growth |
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