CN104138547A - Application of coix seed water extract in preparing estrogen drugs - Google Patents
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Abstract
The invention discloses application of coix seed water extract in preparing estrogen drugs. According to the coix seed water extract, coix seeds are soaked in water with the weight 10 times to 12 times that of the coix seeds for 0.5 h to 0.7 h, then, decoction is performed 3 times to 4 times, decoction lasts for 50 min to 60 min each time, decoction liquid is combined, and the coix seed water extract is obtained through vacuum concentration and drying; the coix seed water extract is used for preparing estrogen drugs. Compared with the prior art, the preparing method of the coix seed water extract is simple, stable, reliable, convenient to use and low in cost, the same or similar results are obtained through many experiments, the coix seed water extract is effectively used for preparing estrogen drugs, new purposes of coix seed drugs are developed, and the clinical significance is large.
Description
Technical field
The present invention relates to field of medicaments, the particularly application of a kind of Semen Coicis water extract in preparing estrogens medicine, can be effective to estrogen hyposecretion in preparation treatment body and the relevant disease that causes, as climacteric syndrome etc.
Background technology
Semen Coicis is grass Semen Coicis
coixlacroyma-jobi L. var. ma-yuen (Roman.) Stapfdry mature kernal.< < Compendium of Materia Medica > > classifies as top grade, and wild survivor is few, and main is cultivation.It is cool in nature, and sweet in the mouth, light is returned spleen, stomach, lung meridian.There is promoting diuresis to eliminate damp pathogen, invigorating the spleen to arrest diarrhea, eliminating impediment, evacuation of pus, the effect of detoxicating and resolving stagnation of pathogens.Cure mainly edema, beriberi, dysuria, diarrhea due to hypofunction of the spleen, arthralgia chiefly caused by damp pathogen contracture, lung abscess, acute appendicitis, the diseases such as cancerous protuberance.Modern pharmacological research shows that Semen Coicis has antitumor, enhancing human body immunity power, and antiinflammatory, blood sugar lowering, suppresses the effects such as Skeletal Muscle Contraction and calmness, analgesia.But not yet relevant for the research of Semen Coicis estrogenic activity aspect, report.
Summary of the invention
For problems of the prior art, object of the present invention is just to provide the application of a kind of Semen Coicis water extract in preparing estrogens medicine, can effectively solve the medication problem due to the relevant disease (as climacteric syndrome etc.) that in body, estrogen hyposecretion causes.And provide reference to clinician, while being intended to drug matching, consider that this factor improves prescription curative effect.
For achieving the above object, the technical solution used in the present invention is:
The application of Semen Coicis water extract in preparing estrogens medicine, described Semen Coicis water extract is for the preparation of estrogens medicine.
Described Semen Coicis water extract is that Semen Coicis is first soaked to 0.5-0.7h with the water of its 10-12 times weight at every turn, then decocts 3-4 time, decocts 50-60min at every turn, merges decoction liquor, the dry Semen Coicis water extract that obtains of concentrating under reduced pressure; Described Semen Coicis water extract is for the preparation of estrogens medicine, this Semen Coicis water extract can be effective to preparation treatment due to the medicine of the relevant disease that in body, estrogen hyposecretion causes, realize Semen Coicis water extract preparation treatment due to body in application in the relevant disease that causes of estrogen hyposecretion (as climacteric syndrome etc.) medicine.
Compared with prior art, water extract preparation method of the present invention is simple, reliable and stable, and through test of many times, has all obtained identical or akin result, and medicine is easy to prepare, and cost is low.Its water extract is effective to prepare estrogens medicine, has opened up the medicinal new purposes of Semen Coicis, and clinical meaning is huge.
Accompanying drawing explanation
Fig. 1 be in the embodiment of the present invention 3 Semen Coicis water extract on the impact of MCF-7 propagation (
± s, n=6) schematic diagram, note: with the comparison of blank group, # represents P<0.05, and ## represents P<0.01; With the comparison of positive group, * represents P<0.05, and * * represents P<0.01.
Fig. 2 be in the embodiment of the present invention 3 Semen Coicis water extract on the impact of sex immature Mouse Uterus coefficient (
± s, n=10) schematic diagram, note: with the comparison of blank group, # represents P<0.05, and ## represents P<0.01; With the comparison of positive group, * represents P<0.05, and * * represents P<0.01.
Fig. 3 is when in the embodiment of the present invention 3, ER is alpha mediated, the reporter gene Luc transient expression result that Semen Coicis water extract regulates and controls ERE (
± s, n=3) schematic diagram, note: with the comparison of blank group, # represents P<0.05, and ## represents P<0.01; With the comparison of positive group, * represents P<0.05, and * * represents P<0.01.
Fig. 4 is when in the embodiment of the present invention 3, ER is beta mediated, the reporter gene Luc transient expression result that Semen Coicis water extract regulates and controls ERE (
± s, n=3) schematic diagram, note: with the comparison of blank group, # represents P<0.05, and ## represents P<0.01; With the comparison of positive group, * represents P<0.05, and * * represents P<0.01.
The specific embodiment
Below in conjunction with embodiment and test related data, concrete condition of the present invention is elaborated.
The present invention in concrete enforcement, the application of described Semen Coicis water extract in preparing estrogens medicine, this Semen Coicis water extract is to be provided by following examples:
Embodiment 1
The material that the present embodiment is used and instrument:
1. experiment material
1.1 Experimental agents and main agents
Semen Coicis is grass Semen Coicis
coixlacroyma-jobi L. var. ma-yuen (Roman.) Stapfdry mature kernal.Tap plant during fruit maturation autumn, dries, and lays fruit, then dry, and except decapsidate, yellowish-brown kind skin and impurity, collects kernel, is Semen Coicis crude drug; Reagent is domestic analytical pure.
1.2 key instrument
Pure water instrument (Sartorius 611VF); 90-3 magnetic stirring apparatus (Shanghai Zhen Jie experimental facilities company limited); ZRD-7080 full-automatic novel air dry oven (Shanghai ZHICHENG Anaiytical Instrument Manufacturing Co., Ltd.); AB204-N electronical reading analytical balance (Mettler-Toledo Instrument's product); SK6200H type ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.).
The present embodiment Semen Coicis water extract is to use the water 1000g (being 1000ml) of its 10 times of weight first to soak 0.5h Semen Coicis 100g at every turn, then decoct 3 times, each 50min that decocts, merge three times decocting liquid, the dry Semen Coicis water extract that obtains of concentrating under reduced pressure, average yield is 10.38%, this water extract can be prepared estrogens medicine, be used for the treatment of due to the disease that in body, estrogen hyposecretion causes, as climacteric syndrome medicine.
Embodiment 2
The material that the present embodiment is used and instrument:
1. experiment material
1.1 Experimental agents and main agents
Semen Coicis is grass Semen Coicis
coixlacroyma-jobi L. var. ma-yuen (Roman.) Stapfdry mature kernal.Tap plant during fruit maturation autumn, dries, and lays fruit, then dry, and except decapsidate, yellowish-brown kind skin and impurity, collects kernel, is Semen Coicis crude drug; Reagent is domestic analytical pure.
1.2 key instrument
Pure water instrument (Sartorius 611VF); 90-3 magnetic stirring apparatus (Shanghai Zhen Jie experimental facilities company limited); ZRD-7080 full-automatic novel air dry oven (Shanghai ZHICHENG Anaiytical Instrument Manufacturing Co., Ltd.); AB204-N electronical reading analytical balance (Mettler-Toledo Instrument's product); SK6200H type ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.).
The present embodiment Semen Coicis water extract is to use the water 5500g (being 5500ml) of its 11 times of weight first to soak 0.6h Semen Coicis 500g at every turn, then decoct 3 times, each 1h, merge three times decocting liquid, the dry Semen Coicis water extract that obtains of concentrating under reduced pressure, average yield is 10.98%, this water extract can be prepared estrogens medicine, be used for the treatment of due to the disease that in body, estrogen hyposecretion causes, as climacteric syndrome medicine.
Embodiment 3
The present embodiment Semen Coicis water extract is to use the water 12000g (being 12000ml) of its 12 times of weight first to soak 0.7h Semen Coicis 1000g at every turn, then decoct 4 times, each 1h, merge four times decocting liquid, the dry Semen Coicis water extract that obtains of concentrating under reduced pressure, average yield is 11.62%, this water extract can be prepared estrogens medicine, be used for the treatment of due to the disease that in body, estrogen hyposecretion causes, as climacteric syndrome medicine.
According to the method in the present embodiment, in the ratio of Semen Coicis and water, can make by suitability for industrialized production the Semen Coicis water extract of any amount, for the preparation of estrogens medicine, effectively solve the medication problem due to disease that in body, estrogen hyposecretion causes (as climacteric syndrome etc.), and obtained fully proving through test.
The present embodiment testing data is as follows:
The estrogen-like effects that adopts cell proliferation experiment, zoopery and reporter gene technical research Semen Coicis water extract, its related tests data is as follows:
First, by MCF-7 cell proliferation experiment (E-SCREEN), find that Semen Coicis water extract can promote the propagation of MCF-7 cell, points out it to have in vitro estrogen-like effects.
Secondly, by the Mouse Uterus experiment of increasing weight, prove that Semen Coicis water extract can significantly increase the uterus coefficient of sex immature female mice, points out it to have in vivo estrogen-like effects.
Finally, the reporter gene transient expression regulating and controlling by ERE detects, and further illustrating Semen Coicis water extract is by bringing into play estrogen-like effects with the combination of estrogen receptor ER α and ER β.
The present embodiment has proved the new purposes of medicine of the present invention in preparing estrogens medicine by experiment, and its main scheme for implementing said method is as follows:
one, experiment material and method
1. Experimental agents
Semen Coicis is grass Semen Coicis
coixlacroyma-jobi L. var. ma-yuen (Roman.) Stapfdry mature kernal.Tap plant during fruit maturation autumn, dries, and lays fruit, then dry, and except decapsidate, yellowish-brown kind skin and impurity, collects kernel, is Semen Coicis crude drug.
2. laboratory animal and cell strain and plasmid
Kunming mouse, female, be born 21 days (just weaning), body weight 9～12g, is purchased from Henan Province's Experimental Animal Center.Human breast cancer cell (MCF-7) is purchased from cell resource center of Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.HEK293 cell strain is purchased from Chinese Typical Representative culture collection center.Beta galactosidase (β-galactosidase, β-gal) control plasmid p β gal-Control, restructuring reporter gene pERE-TAL-luc are so kind as to give by the dense doctor of leaf chess of BIO ENGINEERING INST MILITARY.Recombinant human ER α (humanER α, hER α) expression vector pCXN2-hER α and recombinant human ER β (humanER β, hER β) Satoshi doctor Inoue of expression vector pCXN2-hER βYou Tokyo University department of medial science are so kind as to give.
3. main agents
DMEM high glucose medium and cationic-liposome LipofectamineTM2000 Reagent are purchased from Gibco Invitrogen company; Hyclone is purchased from Guangzhou Ilex purpurea Hassk.[I.chinensis Sims company; Without phenol red DMEM high glucose medium, glucosan-active carbon, go hormone hyclone purchased from Hyclone company; 17 beta estradiols (17 β-estrogen, E2), ampicillin (Amp), Tris alkali are all purchased from Sigma company; Estradiol valerate tablet (Bayer medicine); O-Nitrobenzol-β-D-synthesis (O-Nitrophenyl-β-D-galactopyranoside, ONPG), EDTA, MTT and DMSO are Amresco company product; Steady-Glo stablizes luciferase detection system test kit (Steady-Glo Luciferase Assay Systerm), glittering lysis buffer (Glolysis Buffer) purchased from Promega company; Yeast powder (OXOID company); Tryptone (OXOID company); Agarose (Biowest company); Competence bacillus coli DH 5 alpha (TransGen company); The little extraction reagent kit of plasmid (Tian Gen company); All the other reagent are domestic analytical pure.
4. key instrument used
General surgery apparatus; CO2 gas incubator (STIK); Inverted microscope (NIKON ECLIPSE TS100); SORVALL ST16R Centrifuge high-speed low temperature refrigerated centrifuger (Thermo Fisher Scientific company); Microplate reader (BIO-RAD 680); Pure water instrument (Sartorius 611VF); 90-3 magnetic stirring apparatus (Shanghai Zhen Jie experimental facilities company limited); ZRD-7080 full-automatic novel air dry oven (Shanghai ZHICHENG Anaiytical Instrument Manufacturing Co., Ltd.); Micro sample adding appliance (Nichipet EX PLUS); AB204-N electronical reading analytical balance (Mettler-Toledo Instrument's product); 10cm culture dish, 96 well culture plates, cryopreservation tube are Corning company and produce; Superclean bench (Jiangsu Su Jing group); Bio Mate 3S ultraviolet spectrophotometer (Thermo Fisher Scientific company); VeritasTM microplate photometer (TurnerBioSystems company); SK6200H type ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.); LRH series biochemical cultivation case (Shanghai Yi Heng Science and Technology Ltd.).
5. experimental technique
5.1 MCF-7 cell proliferation experiment
MCF-7 cell (human breast cancer cell) is through containing 5% and go the DMEM culture medium culturing of estrogen serum after 1 week without phenol red, choose exponential phase cell, PBS washes twice, with after 0.05% trypsinization, add without phenol red DMEM culture medium piping and druming evenly, with 6 * 10
4the concentration of individual/ml is inoculated in 96 orifice plates, and it is 200 μ l that cumulative volume is cultivated in every hole.Cultivate 24h after cell attachment, be changed to pastille culture fluid and continue to cultivate.37 ℃, 5%CO2 are cultivated after 48h, and every hole adds MTT solution (5mg ml
-1) 20 μ l, 37 ℃ are continued to cultivate 4h, the culture fluid that carefully exhausts, and every hole adds 150 μ lDMSO, and concussion 5-10min, dissolves purple crystal completely.With DMSO zeroing, by microplate reader, under 490nm, measure each hole absorbance (A), calculate average A value and the rate of increase (Proliferation Rate, RP).Test in triplicate parallel.
RP%=(experimental group A value-blank group A value) * blank group A value
-1* 100%
5.2 Mouse Uterus weightening finish experiments
Mice is divided into groups by the balanced and random principle of body weight.Administration continues 7 days.Blank group distilled water every day 0.2ml 10g
-1gavage; Positive controls estradiol valerate every day (0.33mg kg
-1d
-1) gavage; Semen Coicis water extract is low dosage by 20 times of amount * extraction ratios of clinical application, and 40 times of amount * extraction ratios of clinical application are that high dose calculates, and are made into the suspension of respective concentration, press 0.2ml 10g every day
-1gavage.After last administration 24h, de-neck is put to death mice, wins immediately uterus and weighs, and calculates uterus coefficient (uterus weight in wet base * (body weight)
-1* 100%).Test in triplicate parallel.Result is compared with blank group, calculates
pvalue, investigates and has not statistically significant.
The reporter gene transient expression of 5.3 ERE regulation and control detects
5.3.1 cell inoculation
Before transfection, 24h is inoculated in 24 orifice plates by the HEK293 cell (HEKC) in exponential phase, and density is 16.0 * 10
4individual/0.5ml/ hole, and culture fluid for containing 10% go estrogen serum not containing antibiotic without phenol red DMEM culture fluid, blank hole, estradiol hole and medicine to be measured hole are set respectively.
5.3.2 the transfection of plasmid
(1), when cell cloth ware 80%-90%, the careful former culture medium of sucking-off, adds 420 μ l to go not containing of estrogen serum antibiotic without phenol red DMEM culture medium containing 10%.
(2) get 3 EP pipes, in ER alphatrons, add 490 μ l without phenol red DMEM culture medium, 9 μ lpCXN2-hER α plasmid (80ng μ l
-1), 9 μ lpERE-TAL-luc plasmid (400ng μ l
-1), 9 μ lp β gal-Control plasmid (200ng μ l
-1); In ER β pipe, add 490 μ l without phenol red DMEM culture medium, 9 μ lpCXN2-hER β plasmid (80ng μ l
-1), 9 μ lpERE-TAL-Iuc plasmid (400ng μ l
-1), 9 μ l β gal-Control plasmid (200ng μ l
-1); In liposome pipe, add 1000 μ l without phenol red DMEM culture medium and 36 μ l liposomees.
(3) reversing mixes liposome pipe, and room temperature is placed 5min, gets 510 μ l and adds respectively ER α and ER β pipe, mixes gently, and room temperature is placed 20min;
(4) get liposome/DNA complex in 80 μ lER alphatrons and add respectively 24 orifice plate ER α experimental ports, get liposome/DNA complex in 80 μ lER β pipes and add respectively 24 orifice plate ER β experimental ports.Front and back shake up, and put into incubator.
(5) after 4-6h, add control drug and detection of drugs.
5.3.3 the reporter gene transient expression of ERE regulation and control detects
The cell of 24h after dosing takes out cell from incubator, and equilibrium at room temperature 20min, sucks culture fluid.According to Steady-Glo, stablize luciferase detection system test kit (Steady-Glo Luciferase Assay Systerm) and the operation of glittering lysis buffer (Glolysis Buffer) evolutionary operation step, collecting cell cracking supernatant, add luciferase reaction substrate (fluorescein), be placed in dark place, by VeritasTM microplate photometer fluorescence intensity.
5.3.4 β-gal is active detects
In order to investigate transfection efficiency, internal reference β-gal activity is detected.
(1) 54 μ l beta-mercaptoethanol adds in 40ml Z-buffer, mixes, and gets EP pipe, and every pipe adds 1800 μ l;
(2) every pipe adds 20 μ l cracking supernatant;
(3) every pipe adds 400 μ l ONPG, and reversing mixes;
(4) place 37 ℃ of incubators, to displaing yellow;
(5) add Na
2cO
31ml cessation reaction;
(6) survey OD420 value;
(7) uciferase activity of normalized;
two, statistical disposition
Experimental data with
± s represents, SPSS18.0 carries out one factor analysis of variance statistical disposition.
three, experimental result
1. administration Semen Coicis water extract impact on MCF-7 cell proliferation after 48 hours
Result shows, Semen Coicis water extract 1,0.1,0.01,0.001,0.0001,0.00001mg ml
-1time with blank group ratio, MCF-7 cell is all had to extremely significant proliferation (P<0.01).Prompting Semen Coicis water extract has estrogenic activity in vitro.In Table 1, Fig. 1.
The impact that table 1 Semen Coicis water extract is bred MCF-7 (
± s, n=6)
Note: with the comparison of blank group, ☆ represents P<0.05, and ★ represents P<0.01; With the comparison of positive group, △ represents P<0.05, ▲ expression P<0.01.
2. the impact of Semen Coicis water extract on Mouse Uterus coefficient
Result demonstration, positive drug estradiol valerate and Semen Coicis water extract low dosage are compared all the extremely significantly increase (P<0.01) of inductivity immaturity Mouse Uterus coefficient with blank group with high dose.Prompting Semen Coicis water extract has estrogenic activity in vivo.In Table 2, Fig. 2.
Table 2 Semen Coicis water extract on the impact of sex immature Mouse Uterus coefficient (
± s, n=10)
Note: with the comparison of blank group, ☆ represents P<0.05, and ★ represents P<0.01; With the comparison of positive group, △ represents P<0.05, ▲ expression P<0.01.
3. the reporter gene transient expression of ERE regulation and control detects and whether evaluates Semen Coicis water extract by estrogen receptor approach performance estrogen-like effects
Utilize the interactional impact of reporter gene technology for detection Semen Coicis water extract and estrogen receptor (Estrogen Receptors, ERs).
Positive controls i.e. 17 beta estradiol (E
2) final concentration that adds is 10
-8mol L
-1, Semen Coicis water extract adding consistency is respectively 1mg ml
-1, 0.1mg ml
-1, 0.01mg ml
-1, 0.001mg ml
-1.Result demonstration, no matter be beta mediated by ER α or ER, E
2uciferase activity after standardization is all significantly higher than blank well (P<0.01).When alpha mediated by ER, Semen Coicis water extract 1mg ml
-1the uciferase activity utmost point after standardization is significantly higher than normal hole (P<0.01), 0.1mg ml
-1uciferase activity after standardization is significantly higher than normal hole (P<0.05), table 3, Fig. 3.When beta mediated by ER, Semen Coicis water extract 1mg ml
-1with 0.01mg ml
-1uciferase activity after two standard sets also all the utmost point be significantly higher than blank well (P<0.01), Semen Coicis water extract 0.01mg ml
-1, 0.001mg ml
-1uciferase activity after standardization is significantly higher than normal hole (P<0.05), table 4, Fig. 4.Illustrate that Semen Coicis water extract can pass through estrogen receptor ER α and the beta mediated activated gene of ER be transcribed performance estrogen-like effects, and can find out that by experimental result Semen Coicis water extract is mainly by the beta mediated performance estrogen-like effects of ER.
Table 3 Semen Coicis water extract by ER alpha mediated to the reporter gene transient expression testing result of ERE regulation and control (
± s, n=3)
Note: with the comparison of blank group, ☆ represents P<0.05, and ★ represents P<0.01; With the comparison of positive group, △ represents P<0.05, ▲ expression P<0.01.
Table 4 Semen Coicis water extract by ER beta mediated to the reporter gene transient expression testing result of ERE regulation and control (
± s, n=3)
Note: with the comparison of blank group, ☆ represents P<0.05, and ★ represents P<0.01; With the comparison of positive group, △ represents P<0.05, ▲ expression P<0.01.
The demonstration of MCF-7 cell proliferation experiment result, Semen Coicis water extract is 1mg ml in concentration
-1, 0.1mg ml
-1, 0.01mg ml
-1, 0.001mg ml
-1, 0.0001mg ml
-1with, 0.00001mg ml
-1time MCF-7 cell is all had to significant proliferation.
Mouse Uterus weightening finish experimental result shows, Semen Coicis water extract can significantly increase sex immature Mouse Uterus coefficient, illustrate Semen Coicis water extract in vivo, externally all there is estrogenic activity.
The reporter gene transient expression testing result demonstration of ERE regulation and control, the estrogen-like effects of Semen Coicis water extract mediates performance jointly by ER α and ER β, and mainly by the beta mediated performance estrogen-like effects of ER.
In sum, animal and the demonstration of cell experiment result, Semen Coicis water extract has certain estrogen-like effects, and is mainly by the beta mediated performance estrogen-like effects of ER.
four, conclusion
MCF-7 cell is the human breast cancer cell strain of estrogen receptor positive, can be subject to specifically estrogen or estrogenic activity material regulate and breed, and is the cell strain of the most frequently used detection estrogenic activity.MCF-7 cell estrogen-like proliferation test is very sensitive, can distinguish estrogen agonist and antagonist, thereby is widely used in external a large amount of, rapid screening and evaluation environmental estrogens and phytoestrogen.In experiment MCF-7 cell proliferation being affected at Semen Coicis water extract, Semen Coicis water extract is 1mg ml in concentration
-1, 0.1mg ml
-1, 0.01mg ml
-1, 0.001mg ml
-1, 0.0001mg ml
-1with, 0.00001mg ml
-1time MCF-7 cell is had to significant proliferation, illustrate that Semen Coicis water extract has estrogenic activity in vitro.
Uterus weight experiment is the classical way of the detection estrogen activity set up the earliest, when evaluating estrogen activity, it considered material absorption, metabolism, be combined with plasma protein and the many factors such as pharmacokinetics, directly reflect the total Biology effect of tested medicine after internal metabolism.Therefore, this experiment adopts sex immature female mice uterus weight in wet base and ratio (uterus coefficient) conduct of body weight to evaluate the index of estrogenic activity.By above-mentioned experiment, Semen Coicis water extract can significantly increase sex immature Mouse Uterus coefficient, and prompting Semen Coicis water extract has estrogen-like effects in vivo.
Above-mentioned test is carried out repeatedly repeatedly, has all obtained identical or close result, prompting, Semen Coicis water extract in vivo, externally all there is certain estrogen-like effects.
Reporter gene (reporter gene) is protein that a kind of coding can be detected or the gene of enzyme, its expression product of a class be very easy to identified, and with endogenous background albumen more easily distinguish gene.Reporter gene technology is about to response element and is connected with reporter gene, by examining report activity of gene expression, and known signal transduction pathway and on the regulation and control of gene expression in cell and impact.Reporter gene sensitivity is high, selectivity is strong and simple to operate.In Semen Coicis water extract is tested the inducing action of Reporter gene vector pERE-TAL-luc transient expression, by the expression of visual report gene, be whether the height of fluorescence intensity can detect tested material and can be combined with estrogen receptor, and act on the expression that estrogen response element (ERE) carrys out controlling gene, thereby the power of understanding Semen Coicis water extract estrogen-like effects with and with the size of ER α or ER β affinity.Experimental result demonstration, when alpha mediated by ER, Semen Coicis water extract 1mg ml
-1the uciferase activity utmost point after standardization is significantly higher than normal hole (P<0.01), 0.1mg ml
-1uciferase activity after standardization is significantly higher than normal hole (P<0.05).When beta mediated by ER, Semen Coicis water extract 1mg ml
-1with 0.01mg ml
-1uciferase activity after two standard sets also all the utmost point be significantly higher than blank well (P<0.01), Semen Coicis water extract 0.01mg ml
-1, 0.001mg ml
-1uciferase activity after standardization is significantly higher than normal hole (P<0.05).Prompting, Semen Coicis water extract can be transcribed through Mediated by Estrogen Receptor activated gene, and is mainly by the beta mediated performance estrogen-like effects of ER.
Organism is a complicated system, and vivo and vitro result exists certain deviation.In this experimental applications In vitro cell experiment, body, Integral animal experiment and reporter gene technology combine and jointly verify the estrogenic activity of tested material, have guaranteed the accuracy of result.
By above-mentioned experiment, proof Semen Coicis water extract can promote the propagation of MCF-7 cell and increase the weight in wet base of sex immature Mouse Uterus, simultaneously Semen Coicis water extract can also activate by ER α and the beta mediated genetic transcription of ER and bring into play estrogenic activity, and is mainly by the beta mediated performance estrogen-like effects of ER.From above experimental result, Semen Coicis water extract has estrogen-like effects, the medicine of the climacteric syndrome causing so it can be effective to estrogen hyposecretion in preparation treatment body.
Claims (2)
1. the application of Semen Coicis water extract in preparing estrogens medicine, is characterized in that: described Semen Coicis water extract is for the preparation of estrogens medicine.
2. the application of Semen Coicis water extract according to claim 1 in preparing estrogens medicine, it is characterized in that: described Semen Coicis water extract is that Semen Coicis is first soaked to 0.5-0.7h with the water of its 10-12 times weight at every turn, then decoct 3-4 time, each 50-60min that decocts, merge decoction liquor, the dry Semen Coicis water extract that obtains of concentrating under reduced pressure; Described Semen Coicis water extract is for the preparation of estrogens medicine.
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| 陆雅丽等: "薏苡仁综合开发利用", 《中国食物与营养》 * |
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Application publication date: 20141112 |