CN104131097A - Method for detecting beef cattle uncoupling protein 3 (UCP3) gene single nucleotide polymorphism (SNP) and use thereof - Google Patents
Method for detecting beef cattle uncoupling protein 3 (UCP3) gene single nucleotide polymorphism (SNP) and use thereof Download PDFInfo
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Abstract
The invention discloses a method for detecting beef cattle uncoupling protein 3 (UCP3) gene single nucleotide polymorphism (SNP). The method comprises that UCP3 gene-containing beef cattle whole genome DNAs to be detected are used as templates and are subjected to PCR amplification by a primer pair P as primers so the beef cattle UCP3 gene is amplified, the PCR product is digested by a restriction endonuclease BglI, the digested PCR product is subjected to agarose gel electrophoresis, and the genotype of the beef cattle UCP3 gene at the 4912th site is identified according to the electrophoresis result. The UCP3 gene functions are related to characters of backfat thickness, and an analysis of relationship between the genotype data and the character proves that different genotypes of G4912A mutants of the forth exon of the UCP3 gene are signally related to backfat thickness and a GG-type individual has backfat thickness obviously greater than that of a GA-type individual (P<0.05). Therefore, the CC genotype can be used as a candidate molecular genetic marker for improving cattle backfat thichness. The detection method can be used for marker-assistant selection (MAS) of meet characters in beef cattle growth and can accelerate fine breeding.
Description
Technical field
The invention belongs to molecular genetics field, relate to the detection of gene mononucleotide polymorphism (SNP), particularly a kind of method and application thereof that detects beef cattle UCP3 gene mononucleotide polymorphism.
Background technology
Along with expanding economy, people improve day by day for quantity and the quality requirements of beef.Although China has become the third-largest cowboying and Beef production state, high-grade major part depends on import.Therefore improve Meat Performance imperative, approach mainly contains and improves feed nutrition, strengthen feeding and management level and cultivate improved seeds etc., and breeding is prerequisite and the basic substance of beef raising development.People understand the genetic development of Meat Traits and grow accordingly, on the basis of the vital movement regulation rule such as fatty deposits, the research of binding molecule biology aspect, found with Meat Traits have the functional gene of close ties and major gene and and the molecular genetic marker of these gene linkages, select according to the cognation between objective trait and candidate gene genotype, the accuracy that has improved breeding direction, has shortened the breeding time limit.
Single nucleotide polymorphism (single nucleotide polymorphism, SNP), mainly refers in genomic level the caused DNA sequence polymorphism of variation by single core thuja acid.The polymorphism that SNP shows only relates to the variation of single base, and this variation can be caused by the conversion of single base (transition) or transversion (transversion), also can be by due to the insertion of base or disappearance.From the impact of the inherited character on biological, cSNP can be divided into again 2 kinds: one is synonym cSNP (synonymous cSNP), be the aminoacid sequence that the change of the encoding sequence due to SNP does not affect its protein of translating, mutating alkali yl is identical with the implication of mutating alkali yl not; Another kind is non-synonym cSNP (non-synonymous cSNP), refers to that the change of base sequence can make the protein sequence of the translation taking it as source change, thereby has affected the function of protein.This change is often the immediate cause that causes biological character to change.In cSNP, approximately having half is non-synonym cSNP.These SNPs are also significant in the research of population genetic and organic evolution as genetic marker.
The molecular biological molecular marker assisted selection that develops into provides theoretical basis and technical support, by the polymorphism in measuring ability gene site, carry out association analysis to different genotype is individual with its production performance, find out preponderant genotype and advantage allelotrope, for seed selection provides foundation, what in this process, detect polymorphism and gene type is accurately very crucial.Main method has PCR-RFLP technology or PCR-SSCP technology in conjunction with DNA sequencing at present.
PCR-RELP method is to detect the effective technology of SNP, finding to introduce restriction enzyme cutting behind SNP site, then carries out agarose, polyacrylate hydrogel electrophoretic analysis, just can differentiate exactly SNP site.PCR-RELP method not only has the accuracy of DNA sequencing method, has overcome again somewhat expensive, troublesome operation, false-positive shortcoming, and the sequence site of detecting is without singularity requirement.
UCP3 genes encoding uncoupling protein 3(uncoupling protein 3, UCP3), this albumen is one of uncoupling protein superfamily member, is that Boss in 1997 finds in human skeletal's flesh the earliest.Uncoupling protein is embedded on mitochondrial inner membrane, is the mitochondrial proton carrier of Mammals, in oxidative phosphorylation uncoupling, anti-oxidant and energy metabolism regulation and control, plays a significant role.In the molecular breeding of pig, have been found that pig UCP3 gene pairs day weight gain, fatty deposits, feed conversion rate and piggy birth weight have material impact.The ox UCP3 assignment of genes gene mapping is in No. 15 euchromosomes of ox.Sherman etc. (2008) find average daily gain and the local growth efficiency significant correlation of ox UCP3 gene intron 3 one A/G polymorphic and European ox and Great Britain catalo.It is higher than allelotrope B frequency that Li etc. (2010) find in Nanyang cattle, western Shandong ox, Yan Bianniu that UCP3 gene BglI enzyme is cut polymorphic allelotrope A.Association analysis shows that in western Shandong ox, AA type is more excellent than AB type betaglobulin content.Result also shows that UCP3 gene BglI enzyme cuts polymorphic and growth traits significant correlation Nanyang cattle, and AB genotype individuality is better than other individualities, shows that this polymorphic site is relevant to the growth traits of ox.The raising of the mRNA horizontal expression amount of Brennan etc. (2009) discovery UCP3 gene is relevant with the fatty acid by-products that lipid acid β-oxidation and ox body weight loss produce.Detection method provided by the invention is that the foundation of UCP3 gene SNP and Meat Quality relation is laid a good foundation, for use in the marker assisted selection (MAS) of beef cattle Meat Quality, the beef cattle population that Rapid Establishment genetic resources is good.
Summary of the invention
The problem that the present invention solves is to utilize PCR-RELP method to detect the polymorphism of beef cattle UCP3 gene, and itself and Meat Quality are carried out to association analysis, whether checking can be used as the molecule marker of assisted Selection in Molecular Breeding in Beef Cattle, thereby accelerates fine-variety breeding speed.
The present invention is achieved through the following technical solutions:
A method that detects beef cattle UCP3 gene mononucleotide polymorphism, is characterized in that, to comprise the beef cattle complete genome DNA masterplate to be measured of UCP3 gene, and taking primer pair P as primer, pcr amplification beef cattle UCP3 gene; With after restriction enzyme Bgl I digestion PCR product, then amplified fragments after enzyme is cut carries out agarose gel electrophoresis; According to the single nucleotide polymorphism of the 4912nd of agarose gel electrophoresis result qualification beef cattle UCP3 gene.
for detection ofthe primer of beef cattle UCP3 gene mononucleotide polymorphism, is characterized in that: described primer is primer pair p, and sequence is: upstream primer: 5'-TCACTATCCACGACCCTACC-3';
Downstream primer: 5'-GGTGTAGAACTGCTTGACGG-3'.
Described pcr amplification reaction program is: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30s; 60 DEG C of annealing 40s; 72 DEG C are extended 30s; 72 DEG C are extended 7min; 4 DEG C of preservations.
Described agarose gel electrophoresis is 2% agarose gel electrophoresis.
Describedly identify that according to agarose gel electrophoresis result the single nucleotide polymorphism of the 4912nd of beef cattle UCP3 gene is: GG genotype shows as 396/166bp band; GA genotype shows as 560bp/396bp/166bp band; AA genotype shows as 560bp band.
Compared with prior art, the present invention has following useful technique effect:
The present invention is according to the primers of UCP3 gene, taking the genomic dna of 8 beef breeds as masterplate, carries out pcr amplification, and PCR product is checked order respectively, obtains the partial sequence of beef cattle UCP3 gene after order-checking.After comparing with the sequence of NCBI announcement, find to have SNP polymorphism at the 4912nd.
For the SNP polymorphism of above-mentioned the 4912nd, the present invention has also announced its examination and detection method, by designing specific primer, pcr amplification object fragment, then with the qualification of specific digestion with restriction enzyme, can be simply, quick, cost is low, the polymorphism that detects accurately its mononucleotide.
The present invention has carried out detection and genotype frequency analysis to the SNP genotype of 8 kinds of beef breeds, above-mentioned SNP site and beef cattle some growth proterties (Slaughter weight, ketoboidies weight, dressing percentage, meat weight, marbling scoring, eye muscle area, the actual measurement thickness of backfat, tender degree, round is thick, waist flesh is thick, ketoboidies is long) are carried out to association analysis, and result shows that this site can serve as the molecule marker that improves the beef cattle thickness of backfat.
Brief description of the drawings
Fig. 1 is primer P different ox individualities (the 4912nd polymorphic site of ox UCP3 gene) the PCR product agarose gel electrophoresis figure that increases, and wherein M is 600bp Marker, and 1-5 is PCR product.
Fig. 2 is that the ox UCP3 gene order the 4912nd that in the present invention, PCR product mixing order-checking examination is arrived is G or A sudden change sequencing result figure.
Fig. 3 is the rflp analysis figure of ox UCP3 gene primer P amplified fragments PCR product of the present invention.Wherein 1,3,5,10,11,12 swimming lanes are GA type, and 2,4,6,7 swimming lanes are GG type, and 8,9 swimming lanes are AA type, and M is 600bp Marker.
Embodiment
The present invention utilizes PCR-RFLP method to detect the single nucleotide polymorphism of the 4912nd sudden change of beef cattle UCP3 gene, below the present invention is described in further detail, the explanation of the invention is not limited.
The design of A, beef cattle UCP3 gene intron 2 region PCR primer
Taking ox (NC_007313.5) sequence that NCBI was announced as reference, utilize the Primer premier 5.0 software designs PCR product that comprises beef cattle UCP3 gene intron 2 region that can increase, its primer sequence is as follows:
Upstream primer: 5'-TCACTATCCACGACCCTACC-3'
Downstream primer: 5'-GGTGTAGAACTGCTTGACGG-3'
B, carry out the UCP3 gene fragment of pcr amplification beef cattle to be measured with primer P
embodiment 1
1, the acquisition and processing of ox sample
The present invention adopts 8 cows bodies to amount to 318 individualities, be specially: 18 parts, (1) Angus (Angus) bovine blood sample, 23 parts of Jin Nanniu (Jinnan) blood samples, profit wood is praised 22 parts, (Limousin) bovine blood sample, 29 parts of Luxi Yellow cattle (Luxi) blood samples, 27 parts of Qinchuan Cattle (Qinchuan) blood samples, 30 parts, Simmental (Simmental) bovine blood sample, 29 parts, Xia Luolai (Charolais) bovine blood sample, picks up from great Chang Xian Huaan, Hebei cattle fattening farm; (2) 30 parts, Angus (Angus) bovine blood sample, 30 parts, Hareford (Hereford) bovine blood sample, 28 parts, Simmental (Simmental) bovine blood sample, picks up from Tongliao, Inner Mongolia ternary cattle fattening farm; (3) Simmental (Simmental) bovine blood sample, picks up from Tongliao, Inner Mongolia Baolong mountain cattle fattening farm.Take a blood sample 8 mL in 10 mL pipes from the jugular vein of every ox, add ACD antithrombotics (blood and ACD volume ratio are 6:1) and shake up, blood sample is taken back to laboratory with bubble chamber on the rocks, save backup in-80 DEG C of Ultralow Temperature Freezers.
2, the extraction of genomic dna
(1) under frozen blood specimen chamber temperature, melt, shake up, draw 1.5mL whole blood with liquid-transfering gun and add in 5mL centrifuge tube, add 1.5mL(or with blood sample equal-volume) PBS damping fluid, put upside down and mix 10min, the centrifugal 10min of 12000r/min, can see centrifuge tube bottom white corpuscle precipitation after finishing.
(2) abandon supernatant liquor, repeat step 1～2 time of (1), in lower floor's white corpuscle without red corpuscle.
(3) abandon supernatant liquor, add 1mL lysate SET, 40 μ L 10% SDS, 20 μ L Proteinase K solution, vibrator vibration 2～3min, makes it fully to mix, and as for 55 DEG C of water-baths in water bath with thermostatic control vibrator, digestion is spent the night to loseing thickness agglomerate.
(4) Xiang Guanzhong adds and the saturated phenol of the isopyknic Tris of liquid in pipe (about 1.5mL), covers tightly pipe lid, slowly puts upside down continuously to mix to be no less than 10min, the centrifugal 10min of 12000r/min.
(5) carefully draw supernatant liquor to 5 new mL centrifuge tubes, add equal-volume phenol/chloroform/primary isoamyl alcohol (25:24:1) to mix liquid, slowly put upside down centrifuge tube and be no less than 10min, solution two-phase is fully mixed, the centrifugal 10min of 12000r/min.
(6) shift supernatant liquor to another centrifuge tube, add equal-volume chloroform/primary isoamyl alcohol (24:1), mix the centrifugal 10min of 12000r/min.
(7) shift supernatant liquor to new centrifuge tube, add the dehydrated alcohol of 2 times of volume precoolings, cover tightly pipe lid, can see white cotton-shaped DNA precipitation along a horizontal shaken several times of direction, centrifugal 4min, makes DNA be attached to centrifuge tube tube wall bottom.
(8) by 75% washing with alcohol twice of precooling, carefully pour out ethanol, room temperature is placed.
(9) after ethanol volatilization, add 100 μ L ddH
2o dissolves, and 4 DEG C of placements, after 24h, are transferred to DNA solution in the 1.5mL centrifuge tube of sterilizing, and-20 DEG C save backup.
3, DNA concentration and purity detecting
(1) agarose gel electrophoresis detects
Get 5 μ L DNA solutions, mix with 1 μ L sample-loading buffer (6 × loading buffer), in 1% sepharose, with 100V voltage electrophoresis 30min left and right, after finishing, utilize gel imaging system to take pictures, the purity of preliminary observation DNA.
(2) UV spectrophotometer measuring
5 μ l DNA solution to be measured is fully dissolved in to ddH
2o, and be settled to 1ml.Utilize ultraviolet spectrophotometer to record the OD value at its 260nm and 280nm wavelength place.Calculate DNA purity and concentration according to following formula:
DNA concentration=OD
260× 50ng/ μ l × 200
DNA purity=OD
260/ OD
280
If DNA purity is between 1.8-2.0, DNA purity is higher; If be less than 1.8, in sample, may exist RNA to pollute; If be greater than 2.0, in sample, may there is protein contamination.Measuring OD value through ultraviolet spectrophotometer is 1.72, and purity is higher.
4, pcr amplification
Respectively taking the DNA of 8 cattle breeds as masterplate, carry out pcr amplification with above-mentioned primer, PCR total reaction system is 32 μ L, in table 1; PCR total reaction program, in table 2;
Table 1 PCR reaction system of the present invention
* 2 × Taq PCR MasterMix comprises Taq archaeal dna polymerase, dNTPs, MgCl
2, reaction buffer, PCR reaction toughener and optimize agent and stablizer, concentration is 2 ×, specific product composition refers to product description
Table 2 PCR response procedures of the present invention
The detection of C, object fragment
PCR product and nucleic acid sample-loading buffer are fully mixed with 6:1 volume ratio, taking the DNA molecular marker of 600bp as contrast, in the sepharose that is 2% in concentration with about 150v voltage electrophoresis 20min, whether take pictures with gel imaging instrument, be required object fragment according to the big or small preliminary judgement of PCR product.
D, PCR-RFLP detect
Taking sequencing result as foundation, utilize dCAPs database and Primer5.0 software to search suitable restriction enzyme, reaction system and reaction conditions with reference to the suggestion of restriction endonuclease product description digest, and reaction system is in Table 2-8, and reaction conditions is digestion 12h in 37 DEG C of thermostat containers.
Table 3 endonuclease reaction system
Enzyme is cut product taking 600bp DNA molecular marker as contrast, carries out 150V in sepharose, and the electrophoresis of about 20min finishes to take pictures with gel imaging instrument afterwards, judges genotype according to stripe size and segments.
E, order-checking
If the electrophoretogram banding pattern difference of same clip single stranded DNA on sepharose, be considered as genotype variant, every kind of banding pattern chosen 2 samples and carried out pcr amplification, by biotechnology (Shanghai) Co., Ltd., PCR product is carried out to purifying and order-checking, DNA sequence dna on sequencing result and GeneBank database is compared one by one, finally to determine position and the sudden change kind in SNP site.
Because ox is diploid animal, in the time undergoing mutation, can form different genotype, can differentiate by agarose gel electrophoresis figure, judge whether to have occurred point mutation according to the number of band, different genotype is distinguished, thereby detect its SNP polymorphism.Result shows, occurs three kinds of different genotype (GA, GG, AA), as shown in Figure 3 at the 4912nd.
The frequency statistics analysis of F, beef cattle UCP3 gene SNP site
1, gene and gene frequency
Gene frequency (gene frequency) is illustrated in certain allelotrope shared per-cent in all allelotrope of specific gene seat at this allelotrope place in a colony.Genotype frequency (genotype frequency) represents all ratios of individuality of individual Zhan Gai colony of certain specific gene type.Method of calculation are as follows:
(1) gene frequency: P
i=(2ii+ij)/2N P
j=1-P
i
(2) genotype frequency=genotype number of individuals/N
Wherein, P
iand P
jrepresent the allelic frequency of i, j, N represents colony's sum, and ii and ij represent that in colony, genotype is the number of ii, ij individuality.
Genotype and the gene frequency in UCP3 gene 4912 sites in eight kinds of cattle breeds of table 4
By the gene frequency of table 4, in all 8 kind oxen, G is advantage allelotrope, and frequency is all very high, is respectively: Angus 0.7396, Hareford 0.7000, south, Shanxi ox 0.6739, sharp wood praise 0.6136, western Shandong ox 0.5862, Qinchuan Cattle 0.6111, Simmental 0.6636, Xia Luolai 0.7414.From genotype frequency, Angus and Xia Luolai colony all present the trend of GG>GA>AA, and the colony of other 6 kinds is GA>GG>AA.
G,
uCP3gene
g4912Athe association analysis of polymorphism and Meat Traits
Adopt SAS9.1 statistical software, carried out test of significance (in table 7) to the different genotype of 8 cows bodies is individual with proterties data.
1) major traits of measuring comprises: Slaughter weight, ketoboidies weight, dressing percentage, meat weight, marbling scoring, eye muscle area, the actual measurement thickness of backfat, tender degree, round is thick, waist flesh is thick, ketoboidies is long.
2) colony measuring: totally 318 of eight cows bodies.
The model of association analysis general linear: call SAS9.1 software general linear model GLM(genneral linear models procedure) each genotype is carried out to significance detection to Meat Quality data.318 samples that carry out Meat Traits and polymorphism association analysis are respectively from the beef cattle of 8 kinds of 3 cattle farms, and monthly age difference, at Meat Quality Data processing, except considering genotypic effect, also need to consider kind, not, the fixed effect such as monthly age.The statistical model that the comprehensive above influence factor of this experiment is set up is as follows:
Y
abc=μ+G
a+CR
b+M
c+e
abc
In this formula, Y
abcrepresent individual phenotype record; μ is population average; G
afor genotype effect, CR
brepresent group's effect same period, comprise field-effect and variety effect; M
crepresent monthly age effect, e
abcfor random error.Utilize SAS 9.1 statistical softwares according to above-mentioned equation editing operation program, to data analysis, use least square fitting linear model, between each genotype, the index of Meat Traits adopts multiple comparisons (duncan's method), mean number ± standard error for result (X ± SE) represents
pvalue is less than 0.05 for significant difference,
pit is that difference is extremely remarkable that value is less than 0.01.
As shown in Table 5, there is significant correlation in polymorphism and the thickness of backfat in UCP3 gene G4912A mutational site, the thickness of backfat shows as GG type >AA type >GA type, and wherein the individual thickness of backfat of GG type is significantly greater than GA type individuality (P<0.05).
More than illustrate that GG genotype can be used as a candidate molecules genetic marker that improves the meat thickness of backfat.
Table 5
uCP3the association analysis of gene G4912A polymorphism and ox Meat Traits
Note: the different lowercase alphabets of mean number shoulder mark show significant difference (
p<0.05), different capitalizations represent difference extremely significantly (
p<0.01).
pvalue shoulder is designated as * and represents significant difference, and * * represents that difference is extremely remarkable.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any formal and substantial restriction, all those skilled in the art, do not departing within the scope of the technical program, when utilizing the above technology contents, and the equivalent variations of a little change of making, modification, differentiation is equivalent embodiment of the present invention; Meanwhile, change, modification and the differentiation of all foundations essence technology of the present invention to any equivalent variations made for any of the above embodiments, all belongs in the scope of technical scheme of the present invention.
SEQUENCE LISTING
<110> University Of Agriculture and Forestry In Fujian
Mono-kind of <120> detects method and the application thereof of beef cattle UCP3 gene mononucleotide polymorphism
<130> 2
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 20
<212> DNA
<213> artificial sequence
<400> 1
tcactatcca cgaccctacc 20
<210> 2
<211> 20
<212> DNA
<213> artificial sequence
<400> 2
ggtgtagaac tgcttgacgg 20
Claims (6)
1. for detection of a primer for beef cattle UCP3 gene mononucleotide polymorphism, it is characterized in that: described primer is primer pair p, and sequence is: upstream primer: 5'-TCACTATCCACGACCCTACC-3';
Downstream primer: 5'-GGTGTAGAACTGCTTGACGG-3'.
2. a method that detects beef cattle UCP3 gene mononucleotide polymorphism, is characterized in that, to comprise the beef cattle complete genome DNA masterplate to be measured of UCP3 gene, and taking primer pair P as primer, pcr amplification beef cattle UCP3 gene; Use restriction enzyme
bgl Iafter digestion PCR product, then amplified fragments after enzyme is cut carries out agarose gel electrophoresis; According to the single nucleotide polymorphism of the 4912nd of agarose gel electrophoresis result qualification beef cattle UCP3 gene.
3. the way of detection beef cattle UCP3 gene mononucleotide polymorphism according to claim 2, is characterized in that, described pcr amplification reaction program is: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30s; 60 DEG C of annealing 40s; 72 DEG C are extended 30s; 72 DEG C are extended 7min; 4 DEG C of preservations.
4. the way of detection beef cattle UCP3 gene mononucleotide polymorphism according to claim 2, is characterized in that, described agarose gel electrophoresis is 2% agarose gel electrophoresis.
5. according to the way of detection beef cattle UCP3 gene mononucleotide polymorphism as claimed in claim 2, it is characterized in that, identify that according to agarose gel electrophoresis result the single nucleotide polymorphism of the 4912nd of beef cattle UCP3 gene is: GG genotype shows as 396/166bp band; GA genotype shows as 560bp/396bp/166bp band; AA genotype shows as 560bp band.
6. an application for the way of detection beef cattle UCP3 gene mononucleotide polymorphism as claimed in claim 2, is characterized in that: for the marker assisted selection of Beef Cattle Growth Meat Quality and quickening breeding group's seed selection.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257532A (en) * | 2019-07-24 | 2019-09-20 | 吉林大学 | ANGPTL5 gene is as ox back fat thickness molecular labeling and detection method |
| CN111321232A (en) * | 2020-03-18 | 2020-06-23 | 西北农林科技大学 | Method for rapidly detecting copy number variation of EIF4A2 gene of beef cattle and application thereof |
| CN112176070A (en) * | 2020-08-03 | 2021-01-05 | 南京农业大学 | UCP3 gene related to pig intramuscular fat character, molecular marker and application thereof |
| CN113403405A (en) * | 2021-07-31 | 2021-09-17 | 广东海洋大学 | Method for evaluating backfat thickness and shearing force in quality characters of Sichuan yak meat |
-
2014
- 2014-07-28 CN CN201410362112.1A patent/CN104131097B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| E. L. SHERMAN,ET AL: "Polymorphisms and haplotypes in the bovine neuropeptide Y,growth hormone receptor,ghrelin,insulin-like growth factor 2,and uncoupling proteins 2and3 genes and their associations with measures of growth,performance,feed efficiency,and carcass merit in beef", 《JOURNAL OF ANIMAL SCIENCE》, 31 December 2008 (2008-12-31), pages 1 - 16 * |
| R T STONE,ET AL: "Bovine UCP2 and UCP3 map to BTA15", 《ANIMAL GENETICS》, 31 December 1999 (1999-12-31), pages 378 - 381 * |
| 陈翠等: "UCP3 基因遗传多态性与中国西门塔尔牛生长及育肥性状的遗传效应分析", 《中国农学通报》, vol. 28, no. 5, 31 December 2012 (2012-12-31), pages 13 - 18 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257532A (en) * | 2019-07-24 | 2019-09-20 | 吉林大学 | ANGPTL5 gene is as ox back fat thickness molecular labeling and detection method |
| CN111321232A (en) * | 2020-03-18 | 2020-06-23 | 西北农林科技大学 | Method for rapidly detecting copy number variation of EIF4A2 gene of beef cattle and application thereof |
| CN112176070A (en) * | 2020-08-03 | 2021-01-05 | 南京农业大学 | UCP3 gene related to pig intramuscular fat character, molecular marker and application thereof |
| CN113403405A (en) * | 2021-07-31 | 2021-09-17 | 广东海洋大学 | Method for evaluating backfat thickness and shearing force in quality characters of Sichuan yak meat |
| CN113403405B (en) * | 2021-07-31 | 2022-05-24 | 广东海洋大学 | Method for evaluating backfat thickness and shearing force in quality characters of Sichuan yak meat |
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